AlfalfaGEDB | MS.gene75718

M. sativa cultivar XinJiangDaYe / MS.gene75718

KEGG: -
GO: -
PFAM: -

Nr:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene75718.t1	XP_003601603.1	87.3	63	8	0	3	65	5	67	2.20E-18	101.7

Swissprot:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score

Trembl:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene75718.t1	G7J9L5	87.3	63	8	0	3	65	5	67	1.6e-18	101.7

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network:

Gene1	Gene2	correlation coefficient	p_value	FDR
MS.gene055141	MS.gene75718	0.80593	1.05E-49	-1.69E-46
MS.gene061100	MS.gene75718	0.804207	2.41E-49	-1.69E-46

PPI:

Gene1	Gene2	Type

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene75718.t1	MTR_3g083470	80.412	97	17	1	3	97	5	101	3.45e-40	133

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score

Find 19 sgRNAs with CRISPR-Local

Find 27 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
CAATGTCAACACATTGGAAT+TGG	0.350732	3.3:-70115914	MS.gene75718:CDS
AAAGATGACAACAACGATAA+TGG	0.356762	3.3:+70115972	None:intergenic
CAGCTACGGAGAAACAAAAT+TGG	0.368263	3.3:-70115943	MS.gene75718:CDS
CGGATTATCTGAGTTGGAAT+TGG	0.386893	3.3:+70116277	None:intergenic
ATAGATTGTGTATTGTTGTT+TGG	0.388629	3.3:+70116005	None:intergenic
CAATTCCAACTCAGATAATC+CGG	0.409288	3.3:-70116276	MS.gene75718:CDS
TGCTAACAAAACAGCTAAAA+CGG	0.465298	3.3:+70116059	None:intergenic
ACTCAGATAATCCGGCTTCA+AGG	0.465470	3.3:-70116268	MS.gene75718:intron
AAAAGGATCAAAAGCAATGA+AGG	0.473441	3.3:-70116123	MS.gene75718:intron
GCATTGAATTACACAGAAGC+TGG	0.479418	3.3:-70116039	MS.gene75718:CDS
CGAGGTTCTGACCTTGAAGC+CGG	0.497573	3.3:+70116257	None:intergenic
AAGCAATGAAGGAAAGTTGA+AGG	0.508377	3.3:-70116112	MS.gene75718:CDS
TGAAGCCGGATTATCTGAGT+TGG	0.528924	3.3:+70116271	None:intergenic
CAACACATTGGAATTGGAGA+TGG	0.533055	3.3:-70115908	MS.gene75718:CDS
TCATCTTTATTAATCAGCTA+CGG	0.546792	3.3:-70115957	MS.gene75718:CDS
ACTCTTCAAGAAACTGGTGA+TGG	0.557609	3.3:-70115881	MS.gene75718:CDS
CAATGTCAATGTCAACACAT+TGG	0.601927	3.3:-70115920	MS.gene75718:CDS
TGAAGGAAAGTTGAAGGACA+TGG	0.612935	3.3:-70116106	MS.gene75718:CDS
GATGACAACAACGATAATGG+AGG	0.662741	3.3:+70115975	None:intergenic

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!!!	AATCATTTTGTATAAGATTT+TGG	+	chr3.3:70116062-70116081	None:intergenic	15.0%
!	TCATCTTTATTAATCAGCTA+CGG	-	chr3.3:70116185-70116204	MS.gene75718:intron	25.0%
!	TTTGAACTTGAAAGGTAAAA+TGG	+	chr3.3:70115941-70115960	None:intergenic	25.0%
!!	ATAGATTGTGTATTGTTGTT+TGG	+	chr3.3:70116140-70116159	None:intergenic	25.0%
	AAAAGGATCAAAAGCAATGA+AGG	-	chr3.3:70116019-70116038	MS.gene75718:CDS	30.0%
	AAAGATGACAACAACGATAA+TGG	+	chr3.3:70116173-70116192	None:intergenic	30.0%
	TGCTAACAAAACAGCTAAAA+CGG	+	chr3.3:70116086-70116105	None:intergenic	30.0%
!!	ATTGCTTTTGATCCTTTTTG+AGG	+	chr3.3:70116017-70116036	None:intergenic	30.0%
!!	CTAACTTGTTTGAACTTGAA+AGG	+	chr3.3:70115949-70115968	None:intergenic	30.0%
!!	TTGCTTTTGATCCTTTTTGA+GGG	+	chr3.3:70116016-70116035	None:intergenic	30.0%
!!!	TGTTTTGTTTTCCCTCAAAA+AGG	-	chr3.3:70116002-70116021	MS.gene75718:CDS	30.0%
	AAGCAATGAAGGAAAGTTGA+AGG	-	chr3.3:70116030-70116049	MS.gene75718:CDS	35.0%
	CAATGTCAACACATTGGAAT+TGG	-	chr3.3:70116228-70116247	MS.gene75718:intron	35.0%
	CAATGTCAATGTCAACACAT+TGG	-	chr3.3:70116222-70116241	MS.gene75718:intron	35.0%
	CAATTCCAACTCAGATAATC+CGG	-	chr3.3:70115866-70115885	MS.gene75718:CDS	35.0%
	GGAAAAACTCTTCAAGAAAC+TGG	-	chr3.3:70116255-70116274	MS.gene75718:intron	35.0%
	ACTCTTCAAGAAACTGGTGA+TGG	-	chr3.3:70116261-70116280	MS.gene75718:intron	40.0%
	CAACACATTGGAATTGGAGA+TGG	-	chr3.3:70116234-70116253	MS.gene75718:intron	40.0%
	CAATTCATAGTAGTGAAGCG+AGG	+	chr3.3:70115906-70115925	None:intergenic	40.0%
	CAGCTACGGAGAAACAAAAT+TGG	-	chr3.3:70116199-70116218	MS.gene75718:intron	40.0%
	GCATTGAATTACACAGAAGC+TGG	-	chr3.3:70116103-70116122	MS.gene75718:CDS	40.0%
	TGAAGGAAAGTTGAAGGACA+TGG	-	chr3.3:70116036-70116055	MS.gene75718:CDS	40.0%
!	CGGATTATCTGAGTTGGAAT+TGG	+	chr3.3:70115868-70115887	None:intergenic	40.0%
!	GATGACAACAACGATAATGG+AGG	+	chr3.3:70116170-70116189	None:intergenic	40.0%
	ACTCAGATAATCCGGCTTCA+AGG	-	chr3.3:70115874-70115893	MS.gene75718:CDS	45.0%
	TGAAGCCGGATTATCTGAGT+TGG	+	chr3.3:70115874-70115893	None:intergenic	45.0%
!	CGAGGTTCTGACCTTGAAGC+CGG	+	chr3.3:70115888-70115907	None:intergenic	55.0%

Chromosome	Type	Strat	End	Strand	Name
chr3.3	gene	70115858	70116306	70115858	ID=MS.gene75718
chr3.3	mRNA	70115858	70116306	70115858	ID=MS.gene75718.t1;Parent=MS.gene75718
chr3.3	exon	70116269	70116306	70116269	ID=MS.gene75718.t1.exon1;Parent=MS.gene75718.t1
chr3.3	CDS	70116269	70116306	70116269	ID=cds.MS.gene75718.t1;Parent=MS.gene75718.t1
chr3.3	exon	70115858	70116140	70115858	ID=MS.gene75718.t1.exon2;Parent=MS.gene75718.t1
chr3.3	CDS	70115858	70116140	70115858	ID=cds.MS.gene75718.t1;Parent=MS.gene75718.t1

Gene Sequence

>MS.gene75718

ATGGTTTCCAATTCCAACTCAGATAATCCGGCTTCAAGGTCAGAACCTCGCTTCACTACTATGAATTGTTTTTATTTTTACCATTTTACCTTTCAAGTTCAAACAAGTTAGCAATAATAATTAGTTTTTGTACTTTTTTTTGTTTGTTTTGTTTTCCCTCAAAAAGGATCAAAAGCAATGAAGGAAAGTTGAAGGACATGGCCAAAATCTTATACAAAATGATTGCCGTTTTAGCTGTTTTGTTAGCATTGAATTACACAGAAGCTGGAAACATGTTTTCCAAACAACAATACACAATCTATTTCATCTCCTCCATTATCGTTGTTGTCATCTTTATTAATCAGCTACGGAGAAACAAAATTGGCAATGTCAATGTCAACACATTGGAATTGGAGATGGAAAAACTCTTCAAGAAACTGGTGATGGAAAGATATCGATCTCAGATCTGA

Protein sequence

>MS.gene75718.t1

MVSNSNSDNPASRIKSNEGKLKDMAKILYKMIAVLAVLLALNYTEAGNMFSKQQYTIYFISSIIVVVIFINQLRRNKIGNVNVNTLELEMEKLFKKLVMERYRSQI