Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene055141.t1 | XP_003623204.1 | 98.2 | 221 | 4 | 0 | 1 | 221 | 1 | 221 | 7.10E-120 | 439.9 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene055141.t1 | P32110 | 67.3 | 223 | 68 | 2 | 1 | 218 | 1 | 223 | 2.6e-78 | 293.1 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene055141.t1 | G7L6H8 | 98.2 | 221 | 4 | 0 | 1 | 221 | 1 | 221 | 5.1e-120 | 439.9 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene054222 | MS.gene055141 | 0.800995 | 1.11E-48 | -1.69E-46 |
| MS.gene055141 | MS.gene056677 | 0.8292 | 5.88E-55 | -1.69E-46 |
| MS.gene055141 | MS.gene059774 | 0.83098 | 2.16E-55 | -1.69E-46 |
| MS.gene055141 | MS.gene060697 | 0.832338 | 1.00E-55 | -1.69E-46 |
| MS.gene055141 | MS.gene064243 | 0.804103 | 2.54E-49 | -1.69E-46 |
| MS.gene055141 | MS.gene065334 | 0.809054 | 2.29E-50 | -1.69E-46 |
| MS.gene055141 | MS.gene065819 | 0.806311 | 8.75E-50 | -1.69E-46 |
| MS.gene055141 | MS.gene066196 | 0.825476 | 4.59E-54 | -1.69E-46 |
| MS.gene055141 | MS.gene066513 | 0.825409 | 4.76E-54 | -1.69E-46 |
| MS.gene055141 | MS.gene069024 | 0.800897 | 1.16E-48 | -1.69E-46 |
| MS.gene055141 | MS.gene072061 | 0.821144 | 4.72E-53 | -1.69E-46 |
| MS.gene055141 | MS.gene20608 | 0.803249 | 3.81E-49 | -1.69E-46 |
| MS.gene055141 | MS.gene24903 | 0.823312 | 1.48E-53 | -1.69E-46 |
| MS.gene055141 | MS.gene25686 | 0.814653 | 1.38E-51 | -1.69E-46 |
| MS.gene055141 | MS.gene26832 | 0.81362 | 2.33E-51 | -1.69E-46 |
| MS.gene055141 | MS.gene29036 | 0.855739 | 5.15E-62 | -1.69E-46 |
| MS.gene055141 | MS.gene29096 | 0.854557 | 1.14E-61 | -1.69E-46 |
| MS.gene055141 | MS.gene30423 | 0.814575 | 1.44E-51 | -1.69E-46 |
| MS.gene055141 | MS.gene31551 | 0.812073 | 5.09E-51 | -1.69E-46 |
| MS.gene055141 | MS.gene31648 | 0.801661 | 8.10E-49 | -1.69E-46 |
| MS.gene055141 | MS.gene32688 | 0.815766 | 7.81E-52 | -1.69E-46 |
| MS.gene055141 | MS.gene32801 | 0.823649 | 1.24E-53 | -1.69E-46 |
| MS.gene055141 | MS.gene34444 | 0.804864 | 1.76E-49 | -1.69E-46 |
| MS.gene055141 | MS.gene35114 | 0.824696 | 7.02E-54 | -1.69E-46 |
| MS.gene055141 | MS.gene38925 | 0.807212 | 5.64E-50 | -1.69E-46 |
| MS.gene055141 | MS.gene42653 | 0.820895 | 5.39E-53 | -1.69E-46 |
| MS.gene055141 | MS.gene42783 | 0.816036 | 6.80E-52 | -1.69E-46 |
| MS.gene055141 | MS.gene44955 | 0.835566 | 1.56E-56 | -1.69E-46 |
| MS.gene055141 | MS.gene45281 | 0.813899 | 2.03E-51 | -1.69E-46 |
| MS.gene055141 | MS.gene46048 | 0.837002 | 6.72E-57 | -1.69E-46 |
| MS.gene055141 | MS.gene49467 | 0.833552 | 4.99E-56 | -1.69E-46 |
| MS.gene055141 | MS.gene54704 | 0.813879 | 2.05E-51 | -1.69E-46 |
| MS.gene055141 | MS.gene58358 | 0.82988 | 4.02E-55 | -1.69E-46 |
| MS.gene055141 | MS.gene59241 | 0.812533 | 4.04E-51 | -1.69E-46 |
| MS.gene055141 | MS.gene59242 | 0.81277 | 3.59E-51 | -1.69E-46 |
| MS.gene055141 | MS.gene59645 | 0.801807 | 7.56E-49 | -1.69E-46 |
| MS.gene055141 | MS.gene59860 | 0.803558 | 3.29E-49 | -1.69E-46 |
| MS.gene055141 | MS.gene60710 | 0.80497 | 1.67E-49 | -1.69E-46 |
| MS.gene055141 | MS.gene62677 | 0.800687 | 1.28E-48 | -1.69E-46 |
| MS.gene055141 | MS.gene69823 | 0.820158 | 7.95E-53 | -1.69E-46 |
| MS.gene055141 | MS.gene71828 | 0.802842 | 4.63E-49 | -1.69E-46 |
| MS.gene055141 | MS.gene71879 | 0.823562 | 1.30E-53 | -1.69E-46 |
| MS.gene055141 | MS.gene72126 | 0.803525 | 3.34E-49 | -1.69E-46 |
| MS.gene055141 | MS.gene73078 | 0.802631 | 5.12E-49 | -1.69E-46 |
| MS.gene055141 | MS.gene73245 | 0.824671 | 7.12E-54 | -1.69E-46 |
| MS.gene055141 | MS.gene74956 | 0.801172 | 1.02E-48 | -1.69E-46 |
| MS.gene055141 | MS.gene75413 | 0.804043 | 2.61E-49 | -1.69E-46 |
| MS.gene055141 | MS.gene75718 | 0.80593 | 1.05E-49 | -1.69E-46 |
| MS.gene055141 | MS.gene75719 | 0.829321 | 5.49E-55 | -1.69E-46 |
| MS.gene055141 | MS.gene80201 | 0.816439 | 5.53E-52 | -1.69E-46 |
| MS.gene055141 | MS.gene84162 | 0.805294 | 1.43E-49 | -1.69E-46 |
| MS.gene055141 | MS.gene88254 | 0.818361 | 2.04E-52 | -1.69E-46 |
| MS.gene055141 | MS.gene88255 | 0.82934 | 5.44E-55 | -1.69E-46 |
| MS.gene055141 | MS.gene90529 | 0.806866 | 6.68E-50 | -1.69E-46 |
| MS.gene055141 | MS.gene94111 | 0.802074 | 6.67E-49 | -1.69E-46 |
| MS.gene055141 | MS.gene95655 | 0.827246 | 1.74E-54 | -1.69E-46 |
| MS.gene055141 | MS.gene99894 | 0.805698 | 1.18E-49 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene055141.t1 | MTR_7g065680 | 98.190 | 221 | 4 | 0 | 1 | 221 | 1 | 221 | 1.24e-160 | 442 |
| MS.gene055141.t1 | MTR_7g065630 | 85.068 | 221 | 33 | 0 | 1 | 221 | 19 | 239 | 2.25e-137 | 385 |
| MS.gene055141.t1 | MTR_7g065710 | 82.381 | 210 | 34 | 1 | 1 | 210 | 1 | 207 | 3.33e-127 | 358 |
| MS.gene055141.t1 | MTR_7g065700 | 87.143 | 210 | 27 | 0 | 1 | 210 | 1 | 210 | 1.38e-125 | 354 |
| MS.gene055141.t1 | MTR_7g065590 | 65.766 | 222 | 72 | 1 | 1 | 218 | 1 | 222 | 1.51e-103 | 298 |
| MS.gene055141.t1 | MTR_7g065660 | 65.333 | 225 | 74 | 1 | 1 | 221 | 1 | 225 | 6.75e-100 | 289 |
| MS.gene055141.t1 | MTR_7g065600 | 65.315 | 222 | 73 | 1 | 1 | 218 | 1 | 222 | 7.73e-99 | 286 |
| MS.gene055141.t1 | MTR_7g065230 | 67.890 | 218 | 69 | 1 | 1 | 218 | 1 | 217 | 9.89e-99 | 286 |
| MS.gene055141.t1 | MTR_7g065720 | 65.158 | 221 | 72 | 2 | 1 | 216 | 1 | 221 | 9.97e-97 | 281 |
| MS.gene055141.t1 | MTR_7g065740 | 61.062 | 226 | 83 | 2 | 1 | 221 | 1 | 226 | 9.00e-94 | 274 |
| MS.gene055141.t1 | MTR_7g065290 | 62.557 | 219 | 78 | 1 | 1 | 215 | 1 | 219 | 1.33e-93 | 273 |
| MS.gene055141.t1 | MTR_7g065265 | 63.426 | 216 | 75 | 1 | 1 | 212 | 1 | 216 | 2.73e-92 | 270 |
| MS.gene055141.t1 | MTR_7g065750 | 61.504 | 226 | 82 | 2 | 1 | 221 | 1 | 226 | 4.44e-91 | 267 |
| MS.gene055141.t1 | MTR_7g065270 | 60.648 | 216 | 81 | 1 | 1 | 212 | 1 | 216 | 8.72e-89 | 261 |
| MS.gene055141.t1 | MTR_7g065640 | 60.633 | 221 | 73 | 1 | 1 | 221 | 1 | 207 | 6.76e-87 | 256 |
| MS.gene055141.t1 | MTR_4g019790 | 56.889 | 225 | 93 | 1 | 1 | 221 | 1 | 225 | 2.84e-85 | 252 |
| MS.gene055141.t1 | MTR_7g065260 | 61.435 | 223 | 81 | 2 | 1 | 218 | 8 | 230 | 6.50e-83 | 246 |
| MS.gene055141.t1 | MTR_4g019780 | 57.339 | 218 | 87 | 3 | 2 | 214 | 3 | 219 | 2.43e-82 | 244 |
| MS.gene055141.t1 | MTR_6g080440 | 46.154 | 208 | 106 | 3 | 7 | 209 | 3 | 209 | 1.73e-59 | 187 |
| MS.gene055141.t1 | MTR_3g099757 | 47.059 | 221 | 111 | 3 | 5 | 220 | 3 | 222 | 8.79e-58 | 182 |
| MS.gene055141.t1 | MTR_8g087410 | 40.541 | 222 | 126 | 3 | 5 | 221 | 4 | 224 | 3.09e-56 | 178 |
| MS.gene055141.t1 | MTR_8g087425 | 43.662 | 213 | 114 | 3 | 5 | 212 | 3 | 214 | 6.46e-53 | 170 |
| MS.gene055141.t1 | MTR_1g090070 | 41.892 | 222 | 120 | 4 | 7 | 220 | 9 | 229 | 7.79e-52 | 167 |
| MS.gene055141.t1 | MTR_5g040430 | 40.991 | 222 | 119 | 4 | 1 | 211 | 1 | 221 | 7.89e-52 | 167 |
| MS.gene055141.t1 | MTR_1g090150 | 43.458 | 214 | 112 | 4 | 6 | 212 | 2 | 213 | 1.08e-51 | 167 |
| MS.gene055141.t1 | MTR_1g090060 | 41.981 | 212 | 111 | 4 | 7 | 209 | 3 | 211 | 5.90e-50 | 162 |
| MS.gene055141.t1 | MTR_1g115195 | 44.608 | 204 | 102 | 4 | 7 | 201 | 3 | 204 | 2.61e-49 | 160 |
| MS.gene055141.t1 | MTR_4g059730 | 39.252 | 214 | 120 | 4 | 6 | 211 | 3 | 214 | 6.32e-47 | 154 |
| MS.gene055141.t1 | MTR_1g090100 | 41.981 | 212 | 113 | 4 | 8 | 211 | 3 | 212 | 6.89e-47 | 154 |
| MS.gene055141.t1 | MTR_5g037380 | 40.090 | 222 | 121 | 3 | 7 | 216 | 5 | 226 | 5.01e-46 | 152 |
| MS.gene055141.t1 | MTR_2g070130 | 42.439 | 205 | 109 | 6 | 6 | 204 | 3 | 204 | 2.41e-44 | 148 |
| MS.gene055141.t1 | MTR_1g090090 | 39.524 | 210 | 117 | 4 | 7 | 208 | 3 | 210 | 4.13e-44 | 147 |
| MS.gene055141.t1 | MTR_2g070150 | 47.531 | 162 | 80 | 4 | 6 | 165 | 3 | 161 | 7.79e-44 | 146 |
| MS.gene055141.t1 | MTR_2g070140 | 39.286 | 196 | 110 | 5 | 20 | 209 | 1 | 193 | 9.70e-42 | 140 |
| MS.gene055141.t1 | MTR_3g467420 | 40.609 | 197 | 112 | 5 | 16 | 208 | 18 | 213 | 7.25e-41 | 139 |
| MS.gene055141.t1 | MTR_2g070210 | 37.264 | 212 | 122 | 6 | 6 | 209 | 3 | 211 | 1.65e-40 | 138 |
| MS.gene055141.t1 | MTR_4g124130 | 44.737 | 190 | 95 | 6 | 4 | 186 | 1 | 187 | 2.78e-40 | 137 |
| MS.gene055141.t1 | MTR_8g056940 | 47.239 | 163 | 81 | 4 | 6 | 166 | 3 | 162 | 6.21e-40 | 136 |
| MS.gene055141.t1 | MTR_2g070200 | 44.172 | 163 | 87 | 3 | 6 | 166 | 3 | 163 | 8.86e-40 | 136 |
| MS.gene055141.t1 | MTR_8g061950 | 38.249 | 217 | 122 | 4 | 7 | 211 | 5 | 221 | 1.19e-39 | 136 |
| MS.gene055141.t1 | MTR_3g467430 | 37.799 | 209 | 121 | 5 | 6 | 208 | 8 | 213 | 2.43e-38 | 132 |
| MS.gene055141.t1 | MTR_2g070180 | 37.073 | 205 | 120 | 5 | 6 | 204 | 3 | 204 | 3.26e-38 | 132 |
| MS.gene055141.t1 | MTR_2g070120 | 36.453 | 203 | 124 | 4 | 6 | 204 | 3 | 204 | 8.14e-38 | 131 |
| MS.gene055141.t1 | MTR_2g070070 | 35.945 | 217 | 130 | 5 | 7 | 217 | 4 | 217 | 1.27e-37 | 130 |
| MS.gene055141.t1 | MTR_2g070060 | 35.577 | 208 | 125 | 5 | 7 | 208 | 4 | 208 | 1.39e-36 | 128 |
| MS.gene055141.t1 | MTR_5g076900 | 38.164 | 207 | 123 | 4 | 6 | 208 | 5 | 210 | 1.59e-35 | 125 |
| MS.gene055141.t1 | MTR_0186s0030 | 46.429 | 140 | 73 | 2 | 6 | 144 | 3 | 141 | 5.63e-34 | 119 |
| MS.gene055141.t1 | MTR_2g070110 | 36.406 | 217 | 128 | 6 | 7 | 217 | 4 | 216 | 8.74e-34 | 120 |
| MS.gene055141.t1 | MTR_2g072120 | 39.130 | 69 | 40 | 2 | 51 | 118 | 2 | 69 | 6.27e-13 | 62.4 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene055141.t1 | AT3G09270 | 51.584 | 221 | 99 | 5 | 5 | 218 | 4 | 223 | 1.82e-69 | 212 |
| MS.gene055141.t1 | AT2G29420 | 45.455 | 220 | 114 | 3 | 6 | 220 | 8 | 226 | 4.43e-65 | 201 |
| MS.gene055141.t1 | AT2G29450 | 44.749 | 219 | 112 | 4 | 6 | 215 | 5 | 223 | 6.85e-54 | 172 |
| MS.gene055141.t1 | AT2G29480 | 46.119 | 219 | 105 | 5 | 1 | 210 | 1 | 215 | 1.03e-53 | 172 |
| MS.gene055141.t1 | AT2G29490 | 43.750 | 224 | 119 | 4 | 1 | 218 | 1 | 223 | 1.39e-53 | 172 |
| MS.gene055141.t1 | AT2G29460 | 45.370 | 216 | 111 | 4 | 1 | 210 | 1 | 215 | 1.80e-53 | 171 |
| MS.gene055141.t1 | AT2G29470 | 41.333 | 225 | 124 | 5 | 1 | 218 | 1 | 224 | 6.72e-52 | 167 |
| MS.gene055141.t1 | AT2G29440 | 40.909 | 220 | 123 | 4 | 5 | 218 | 4 | 222 | 2.27e-48 | 158 |
| MS.gene055141.t1 | AT1G10370 | 41.232 | 211 | 111 | 4 | 1 | 200 | 1 | 209 | 5.39e-46 | 152 |
| MS.gene055141.t1 | AT1G59700 | 43.913 | 230 | 114 | 7 | 6 | 220 | 5 | 234 | 1.06e-45 | 152 |
| MS.gene055141.t1 | AT1G59670 | 42.512 | 207 | 107 | 5 | 6 | 200 | 5 | 211 | 4.23e-45 | 150 |
| MS.gene055141.t1 | AT1G27140 | 38.667 | 225 | 121 | 5 | 3 | 212 | 2 | 224 | 3.27e-44 | 148 |
| MS.gene055141.t1 | AT1G69930 | 38.532 | 218 | 122 | 5 | 5 | 210 | 10 | 227 | 7.43e-44 | 147 |
| MS.gene055141.t1 | AT1G78340 | 48.214 | 168 | 84 | 3 | 6 | 171 | 3 | 169 | 3.11e-43 | 145 |
| MS.gene055141.t1 | AT1G10360 | 38.350 | 206 | 116 | 4 | 6 | 200 | 4 | 209 | 1.06e-42 | 144 |
| MS.gene055141.t1 | AT1G27130 | 37.838 | 222 | 126 | 5 | 3 | 212 | 2 | 223 | 2.70e-42 | 143 |
| MS.gene055141.t1 | AT1G78320 | 39.130 | 207 | 117 | 5 | 4 | 204 | 1 | 204 | 5.45e-42 | 142 |
| MS.gene055141.t1 | AT1G78370 | 40.394 | 203 | 116 | 5 | 10 | 208 | 7 | 208 | 5.52e-42 | 142 |
| MS.gene055141.t1 | AT1G17170 | 38.889 | 216 | 126 | 5 | 6 | 217 | 3 | 216 | 5.72e-41 | 139 |
| MS.gene055141.t1 | AT1G78380 | 40.196 | 204 | 113 | 5 | 7 | 204 | 4 | 204 | 1.69e-40 | 138 |
| MS.gene055141.t1 | AT1G17180 | 39.901 | 203 | 117 | 5 | 6 | 204 | 3 | 204 | 5.66e-40 | 137 |
| MS.gene055141.t1 | AT1G53680 | 36.493 | 211 | 129 | 5 | 1 | 206 | 1 | 211 | 2.05e-38 | 133 |
| MS.gene055141.t1 | AT5G62480 | 38.627 | 233 | 123 | 8 | 4 | 217 | 1 | 232 | 5.40e-38 | 132 |
| MS.gene055141.t1 | AT1G69920 | 37.799 | 209 | 118 | 5 | 5 | 201 | 32 | 240 | 2.85e-37 | 130 |
| MS.gene055141.t1 | AT1G78360 | 39.070 | 215 | 118 | 7 | 1 | 208 | 1 | 209 | 3.22e-37 | 129 |
| MS.gene055141.t1 | AT1G17190 | 38.172 | 186 | 110 | 5 | 5 | 186 | 3 | 187 | 1.07e-35 | 125 |
| MS.gene055141.t1 | AT3G43800 | 38.049 | 205 | 118 | 7 | 5 | 201 | 3 | 206 | 5.68e-35 | 124 |
| MS.gene055141.t1 | AT1G74590 | 35.961 | 203 | 117 | 5 | 7 | 198 | 7 | 207 | 4.88e-31 | 114 |
| MS.gene055141.t1 | AT1G78320 | 32.039 | 206 | 106 | 5 | 4 | 204 | 1 | 177 | 8.56e-26 | 99.4 |
| MS.gene055141.t1 | AT5G62480 | 31.466 | 232 | 115 | 8 | 4 | 217 | 1 | 206 | 5.30e-21 | 87.4 |
| MS.gene055141.t1 | AT5G62480 | 31.466 | 232 | 114 | 8 | 4 | 217 | 1 | 205 | 5.71e-21 | 87.4 |
Find 39 sgRNAs with CRISPR-Local
Find 118 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TCAAATACAATCCAGTTTAT+AGG | 0.216244 | 7.4:-39292176 | MS.gene055141:CDS |
| ACTGTTTCTCTGCTACTTCT+TGG | 0.238294 | 7.4:+39290417 | None:intergenic |
| TCTGCTACTTCTTGGAATAA+AGG | 0.258191 | 7.4:+39290425 | None:intergenic |
| GTTCCTGTTCTTGTTCATAA+TGG | 0.264740 | 7.4:-39292150 | MS.gene055141:CDS |
| GAGCTAAAGGACAAGTTCTT+TGG | 0.282556 | 7.4:-39290491 | MS.gene055141:CDS |
| AAAGAGCCTTGGATCGGTTT+TGG | 0.296896 | 7.4:-39292041 | MS.gene055141:CDS |
| TGATGAAGAAGTTCAGCTTT+TGG | 0.304952 | 7.4:-39292307 | MS.gene055141:CDS |
| CCTTCAAAGGAGCAACAATT+TGG | 0.305433 | 7.4:-39290314 | MS.gene055141:CDS |
| CTGCTACTTCTTGGAATAAA+GGG | 0.326236 | 7.4:+39290426 | None:intergenic |
| TTCCTGTTCTTGTTCATAAT+GGG | 0.343118 | 7.4:-39292149 | MS.gene055141:CDS |
| CCAAATTGTTGCTCCTTTGA+AGG | 0.353336 | 7.4:+39290314 | None:intergenic |
| TAAGGATCATTAGGCAAGAT+TGG | 0.355374 | 7.4:+39292066 | None:intergenic |
| ATCACACGAGACTCAGAAAT+AGG | 0.380094 | 7.4:+39292123 | None:intergenic |
| ACAGGAACCATCCTATAAAC+TGG | 0.382808 | 7.4:+39292165 | None:intergenic |
| GGCATAACTTCTTTGACAAC+TGG | 0.383415 | 7.4:+39290335 | None:intergenic |
| GTCAAAATTCATAGATGAAA+AGG | 0.387976 | 7.4:-39292019 | MS.gene055141:intron |
| TTCTCGCGCTCTTTCTCATC+AGG | 0.391480 | 7.4:+39290557 | None:intergenic |
| GGGAAGTCCATTTGTGATAA+GGG | 0.397256 | 7.4:-39292277 | MS.gene055141:CDS |
| ATTTGAACCCTTATCACAAA+TGG | 0.404722 | 7.4:+39292270 | None:intergenic |
| TGGGAAGTCCATTTGTGATA+AGG | 0.415245 | 7.4:-39292278 | MS.gene055141:CDS |
| TTCTTTGGAGGACAAGAGAT+TGG | 0.418946 | 7.4:-39290476 | MS.gene055141:CDS |
| TTGAGTATATTGATGAGGCT+TGG | 0.451367 | 7.4:-39292098 | MS.gene055141:CDS |
| AGCTTGGGAAATTTGTCAGC+AGG | 0.453112 | 7.4:+39290389 | None:intergenic |
| TTCCCATTATGAACAAGAAC+AGG | 0.453502 | 7.4:+39292147 | None:intergenic |
| AGGACAAGAGATTGGCTTTG+TGG | 0.456715 | 7.4:-39290468 | MS.gene055141:CDS |
| CAAAGAAGTTATGCCTTCAA+AGG | 0.497169 | 7.4:-39290327 | MS.gene055141:CDS |
| CAAATTGCTCTGAAATTGAA+AGG | 0.499164 | 7.4:-39292252 | MS.gene055141:CDS |
| GTTTCTTGAGAATGAGCTAA+AGG | 0.504881 | 7.4:-39290504 | MS.gene055141:CDS |
| CTAAAGGACAAGTTCTTTGG+AGG | 0.516999 | 7.4:-39290488 | MS.gene055141:CDS |
| TGATCCTTACAAAAGAGCCT+TGG | 0.527046 | 7.4:-39292052 | MS.gene055141:CDS |
| GGCTCGTGAAGAATTATTCG+AGG | 0.551878 | 7.4:-39290534 | MS.gene055141:CDS |
| AGGAATTGAACACAAATATG+TGG | 0.552045 | 7.4:-39292232 | MS.gene055141:CDS |
| TAACTTCTTTGACAACTGGA+TGG | 0.565089 | 7.4:+39290339 | None:intergenic |
| ATACAATCCAGTTTATAGGA+TGG | 0.576358 | 7.4:-39292172 | MS.gene055141:CDS |
| CTTACAAAAGAGCCTTGGAT+CGG | 0.586658 | 7.4:-39292047 | MS.gene055141:CDS |
| GCTTGGGAAATTTGTCAGCA+GGG | 0.597981 | 7.4:+39290390 | None:intergenic |
| TGATGAGAAAGAGCGCGAGA+AGG | 0.601244 | 7.4:-39290555 | MS.gene055141:CDS |
| ATCAGTGTACGATTGCTGCA+TGG | 0.617894 | 7.4:-39290595 | MS.gene055141:intron |
| GATTCTTGAGTATATTGATG+AGG | 0.639329 | 7.4:-39292103 | MS.gene055141:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | AGTTCTATTTATTTATGTTA+TGG | + | chr7.4:39291026-39291045 | None:intergenic | 15.0% |
| !! | ATTTCATTCATGAATTATAT+CGG | - | chr7.4:39290790-39290809 | MS.gene055141:intron | 15.0% |
| !! | GTTCTATTTATTTATGTTAT+GGG | + | chr7.4:39291025-39291044 | None:intergenic | 15.0% |
| !!! | AATTTCACCAAATTTTTTAA+TGG | + | chr7.4:39290773-39290792 | None:intergenic | 15.0% |
| !! | AAAAATGAAATACAAAACAC+AGG | + | chr7.4:39291494-39291513 | None:intergenic | 20.0% |
| !! | CATAACTAAATTTGATCAAT+TGG | + | chr7.4:39291190-39291209 | None:intergenic | 20.0% |
| !! | TTATTTATGTTATGGGTTTA+GGG | + | chr7.4:39291018-39291037 | None:intergenic | 20.0% |
| !! | TTTATTTATGTTATGGGTTT+AGG | + | chr7.4:39291019-39291038 | None:intergenic | 20.0% |
| !!! | CAATTTTCTTTTAACTATAC+TGG | - | chr7.4:39291124-39291143 | MS.gene055141:intron | 20.0% |
| ! | AAATATGTGGAAGAAAAACT+GGG | - | chr7.4:39290367-39290386 | MS.gene055141:CDS | 25.0% |
| ! | ATCAGTAAGAAACAAATATG+TGG | + | chr7.4:39291652-39291671 | None:intergenic | 25.0% |
| ! | ATCATAACAAACGTTTAAAG+TGG | + | chr7.4:39291215-39291234 | None:intergenic | 25.0% |
| ! | CAAATTCATAGATAATCACA+TGG | + | chr7.4:39291833-39291852 | None:intergenic | 25.0% |
| ! | CTGTCAACCATTAAAAAATT+TGG | - | chr7.4:39290763-39290782 | MS.gene055141:intron | 25.0% |
| ! | GATAGTATACATCTCATAAA+AGG | + | chr7.4:39290623-39290642 | None:intergenic | 25.0% |
| ! | GTCAAAATTCATAGATGAAA+AGG | - | chr7.4:39290567-39290586 | MS.gene055141:CDS | 25.0% |
| ! | TCAAATACAATCCAGTTTAT+AGG | - | chr7.4:39290410-39290429 | MS.gene055141:CDS | 25.0% |
| !! | GTTATGATGTTCAGATTTTA+AGG | - | chr7.4:39291227-39291246 | MS.gene055141:intron | 25.0% |
| !! | TTTGTTTCTTACTGATGTAA+TGG | - | chr7.4:39291656-39291675 | MS.gene055141:intron | 25.0% |
| !!! | AATTTTTTAATGGTTGACAG+TGG | + | chr7.4:39290763-39290782 | None:intergenic | 25.0% |
| !!! | GTATTTCGAGTTATAGAAAT+GGG | + | chr7.4:39291573-39291592 | None:intergenic | 25.0% |
| !!! | TCTACTTTAGAATGAATGTT+TGG | - | chr7.4:39291419-39291438 | MS.gene055141:intron | 25.0% |
| !!! | TGTATTTCGAGTTATAGAAA+TGG | + | chr7.4:39291574-39291593 | None:intergenic | 25.0% |
| AAAGTTCACATTTAGTGACA+TGG | + | chr7.4:39291929-39291948 | None:intergenic | 30.0% | |
| AACCAAACATACAGTAGTAT+AGG | - | chr7.4:39291457-39291476 | MS.gene055141:intron | 30.0% | |
| AATTTCCCAAGCTACAAAAA+TGG | - | chr7.4:39292207-39292226 | MS.gene055141:CDS | 30.0% | |
| AGGAATTGAACACAAATATG+TGG | - | chr7.4:39290354-39290373 | MS.gene055141:CDS | 30.0% | |
| ATACAATCCAGTTTATAGGA+TGG | - | chr7.4:39290414-39290433 | MS.gene055141:CDS | 30.0% | |
| ATTTGAACCCTTATCACAAA+TGG | + | chr7.4:39290319-39290338 | None:intergenic | 30.0% | |
| CAAATATGTGGAAGAAAAAC+TGG | - | chr7.4:39290366-39290385 | MS.gene055141:CDS | 30.0% | |
| CTCATATATAGACACAAAAG+AGG | - | chr7.4:39290835-39290854 | MS.gene055141:intron | 30.0% | |
| GATTCTTGAGTATATTGATG+AGG | - | chr7.4:39290483-39290502 | MS.gene055141:CDS | 30.0% | |
| TAACTGCTCTATAATCGTAA+AGG | - | chr7.4:39291047-39291066 | MS.gene055141:intron | 30.0% | |
| TTCCTGTTCTTGTTCATAAT+GGG | - | chr7.4:39290437-39290456 | MS.gene055141:CDS | 30.0% | |
| TTCTTACTGATGTAATGGTA+AGG | - | chr7.4:39291661-39291680 | MS.gene055141:intron | 30.0% | |
| !! | CAAATTGCTCTGAAATTGAA+AGG | - | chr7.4:39290334-39290353 | MS.gene055141:CDS | 30.0% |
| !! | CAGTGGACTATATGTTAAAT+TGG | + | chr7.4:39290746-39290765 | None:intergenic | 30.0% |
| !! | TAATTCGTGTCGTTTGTTAT+GGG | + | chr7.4:39291716-39291735 | None:intergenic | 30.0% |
| !! | TTGGTTTTTAAAAGATGCAG+TGG | - | chr7.4:39291534-39291553 | MS.gene055141:intron | 30.0% |
| AGAAAACAAGAGAAGATCCT+TGG | - | chr7.4:39291601-39291620 | MS.gene055141:intron | 35.0% | |
| ATGGTAGATGAATTCTAGCA+TGG | + | chr7.4:39291910-39291929 | None:intergenic | 35.0% | |
| CAAAGAAGTTATGCCTTCAA+AGG | - | chr7.4:39292259-39292278 | MS.gene055141:CDS | 35.0% | |
| CTCCTATACTACTGTATGTT+TGG | + | chr7.4:39291462-39291481 | None:intergenic | 35.0% | |
| GATGTAATGGTAAGGTTACA+AGG | - | chr7.4:39291669-39291688 | MS.gene055141:intron | 35.0% | |
| GTTCCTGTTCTTGTTCATAA+TGG | - | chr7.4:39290436-39290455 | MS.gene055141:CDS | 35.0% | |
| GTTTCTTGAGAATGAGCTAA+AGG | - | chr7.4:39292082-39292101 | MS.gene055141:CDS | 35.0% | |
| TAACTTCTTTGACAACTGGA+TGG | + | chr7.4:39292250-39292269 | None:intergenic | 35.0% | |
| TAAGGATCATTAGGCAAGAT+TGG | + | chr7.4:39290523-39290542 | None:intergenic | 35.0% | |
| TTCCCATTATGAACAAGAAC+AGG | + | chr7.4:39290442-39290461 | None:intergenic | 35.0% | |
| TTGAGTATATTGATGAGGCT+TGG | - | chr7.4:39290488-39290507 | MS.gene055141:CDS | 35.0% | |
| ! | CTGCTACTTCTTGGAATAAA+GGG | + | chr7.4:39292163-39292182 | None:intergenic | 35.0% |
| ! | GATGAAGAAGTTCAGCTTTT+GGG | - | chr7.4:39290280-39290299 | MS.gene055141:CDS | 35.0% |
| ! | GCTCTTTTGTAAGGATCATT+AGG | + | chr7.4:39290532-39290551 | None:intergenic | 35.0% |
| ! | TCTGCTACTTCTTGGAATAA+AGG | + | chr7.4:39292164-39292183 | None:intergenic | 35.0% |
| ! | TGATGAAGAAGTTCAGCTTT+TGG | - | chr7.4:39290279-39290298 | MS.gene055141:CDS | 35.0% |
| !! | AGACTCCATTTTTGTAGCTT+GGG | + | chr7.4:39292215-39292234 | None:intergenic | 35.0% |
| !! | CTAATTCGTGTCGTTTGTTA+TGG | + | chr7.4:39291717-39291736 | None:intergenic | 35.0% |
| !! | TAGACTCCATTTTTGTAGCT+TGG | + | chr7.4:39292216-39292235 | None:intergenic | 35.0% |
| !! | TCAAGGGTATTGCTGTAATT+TGG | - | chr7.4:39290991-39291010 | MS.gene055141:intron | 35.0% |
| AATTGAGCCTAACACACGTT+CGG | + | chr7.4:39290932-39290951 | None:intergenic | 40.0% | |
| ACAGGAACCATCCTATAAAC+TGG | + | chr7.4:39290424-39290443 | None:intergenic | 40.0% | |
| AGCATGGATCACACATTATG+TGG | + | chr7.4:39291894-39291913 | None:intergenic | 40.0% | |
| ATCACACGAGACTCAGAAAT+AGG | + | chr7.4:39290466-39290485 | None:intergenic | 40.0% | |
| ATCGTAAAGGTAAGCACCAT+TGG | - | chr7.4:39291060-39291079 | MS.gene055141:intron | 40.0% | |
| CCAAATTGTTGCTCCTTTGA+AGG | + | chr7.4:39292275-39292294 | None:intergenic | 40.0% | |
| CCTTCAAAGGAGCAACAATT+TGG | - | chr7.4:39292272-39292291 | MS.gene055141:CDS | 40.0% | |
| CTAAAGGACAAGTTCTTTGG+AGG | - | chr7.4:39292098-39292117 | MS.gene055141:CDS | 40.0% | |
| CTTACAAAAGAGCCTTGGAT+CGG | - | chr7.4:39290539-39290558 | MS.gene055141:CDS | 40.0% | |
| CTTGACACACTTACACAACT+TGG | - | chr7.4:39291515-39291534 | MS.gene055141:intron | 40.0% | |
| GAGCTAAAGGACAAGTTCTT+TGG | - | chr7.4:39292095-39292114 | MS.gene055141:CDS | 40.0% | |
| GGCATAACTTCTTTGACAAC+TGG | + | chr7.4:39292254-39292273 | None:intergenic | 40.0% | |
| GGGAAGTCCATTTGTGATAA+GGG | - | chr7.4:39290309-39290328 | MS.gene055141:CDS | 40.0% | |
| GTGTTAGGCTCAATTCATGT+TGG | - | chr7.4:39290937-39290956 | MS.gene055141:intron | 40.0% | |
| TAGGCTCAATTCATGTTGGA+GGG | - | chr7.4:39290941-39290960 | MS.gene055141:intron | 40.0% | |
| TGATCCTTACAAAAGAGCCT+TGG | - | chr7.4:39290534-39290553 | MS.gene055141:CDS | 40.0% | |
| TGGGAAGTCCATTTGTGATA+AGG | - | chr7.4:39290308-39290327 | MS.gene055141:CDS | 40.0% | |
| TTAGGCTCAATTCATGTTGG+AGG | - | chr7.4:39290940-39290959 | MS.gene055141:intron | 40.0% | |
| ! | ACTGTTTCTCTGCTACTTCT+TGG | + | chr7.4:39292172-39292191 | None:intergenic | 40.0% |
| ! | TTCTTTGGAGGACAAGAGAT+TGG | - | chr7.4:39292110-39292129 | MS.gene055141:CDS | 40.0% |
| ! | TTTTGACCAAAACCGATCCA+AGG | + | chr7.4:39290554-39290573 | None:intergenic | 40.0% |
| AGCTTGGGAAATTTGTCAGC+AGG | + | chr7.4:39292200-39292219 | None:intergenic | 45.0% | |
| ATCAGTGTACGATTGCTGCA+TGG | - | chr7.4:39291991-39292010 | MS.gene055141:intron | 45.0% | |
| GCTTGGGAAATTTGTCAGCA+GGG | + | chr7.4:39292199-39292218 | None:intergenic | 45.0% | |
| GGCTCGTGAAGAATTATTCG+AGG | - | chr7.4:39292052-39292071 | MS.gene055141:CDS | 45.0% | |
| ! | ATAGACACAAAAGAGGCGCA+AGG | - | chr7.4:39290842-39290861 | MS.gene055141:intron | 45.0% |
| ! | CGATCCAAGGCTCTTTTGTA+AGG | + | chr7.4:39290541-39290560 | None:intergenic | 45.0% |
| ! | TAGACACAAAAGAGGCGCAA+GGG | - | chr7.4:39290843-39290862 | MS.gene055141:intron | 45.0% |
| !! | AAAGAGCCTTGGATCGGTTT+TGG | - | chr7.4:39290545-39290564 | MS.gene055141:CDS | 45.0% |
| !! | AGGACAAGAGATTGGCTTTG+TGG | - | chr7.4:39292118-39292137 | MS.gene055141:CDS | 45.0% |
| !!! | AGTTCAGCTTTTGGGTTCAG+TGG | - | chr7.4:39290288-39290307 | MS.gene055141:CDS | 45.0% |
| !!! | GTTCAGCTTTTGGGTTCAGT+GGG | - | chr7.4:39290289-39290308 | MS.gene055141:CDS | 45.0% |
| AAACACTGTCTCCACTGCCA+AGG | + | chr7.4:39291621-39291640 | None:intergenic | 50.0% | |
| CAAGAGAAGATCCTTGGCAG+TGG | - | chr7.4:39291607-39291626 | MS.gene055141:intron | 50.0% | |
| CATGTTGGAGGGAGACTAGT+GGG | - | chr7.4:39290952-39290971 | MS.gene055141:intron | 50.0% | |
| GCACAATCACAGCAGTCACA+TGG | + | chr7.4:39290645-39290664 | None:intergenic | 50.0% | |
| TCATGTTGGAGGGAGACTAG+TGG | - | chr7.4:39290951-39290970 | MS.gene055141:intron | 50.0% | |
| TGTTCACGCAGTTCAGCCAA+TGG | + | chr7.4:39291079-39291098 | None:intergenic | 50.0% | |
| TTCTCGCGCTCTTTCTCATC+AGG | + | chr7.4:39292032-39292051 | None:intergenic | 50.0% | |
| ! | AGACACAAAAGAGGCGCAAG+GGG | - | chr7.4:39290844-39290863 | MS.gene055141:intron | 50.0% |
| ! | TGATGAGAAAGAGCGCGAGA+AGG | - | chr7.4:39292031-39292050 | MS.gene055141:CDS | 50.0% |
| GTTTGGGCCGAACGTGTGTT+AGG | - | chr7.4:39290922-39290941 | MS.gene055141:intron | 55.0% | |
| !! | TTCAGGTGGCGTCGGTTCAA+GGG | - | chr7.4:39290975-39290994 | MS.gene055141:intron | 55.0% |
| ACACGTTCGGCCCAAACGCA+AGG | + | chr7.4:39290919-39290938 | None:intergenic | 60.0% | |
| ACAGGGGGAGGGAACTTTGC+AGG | + | chr7.4:39290896-39290915 | None:intergenic | 60.0% | |
| CAAACGCAAGGACAGGGGGA+GGG | + | chr7.4:39290907-39290926 | None:intergenic | 60.0% | |
| CAGGGGGAGGGAACTTTGCA+GGG | + | chr7.4:39290895-39290914 | None:intergenic | 60.0% | |
| CTCCCCCTGTCCTTGCGTTT+GGG | - | chr7.4:39290906-39290925 | MS.gene055141:intron | 60.0% | |
| TCGGCCCAAACGCAAGGACA+GGG | + | chr7.4:39290913-39290932 | None:intergenic | 60.0% | |
| TTCGGCCCAAACGCAAGGAC+AGG | + | chr7.4:39290914-39290933 | None:intergenic | 60.0% | |
| ! | CACAAAAGAGGCGCAAGGGG+TGG | - | chr7.4:39290847-39290866 | MS.gene055141:intron | 60.0% |
| !! | CTAGTGGGTTCAGGTGGCGT+CGG | - | chr7.4:39290967-39290986 | MS.gene055141:intron | 60.0% |
| !! | GGAGGGAGACTAGTGGGTTC+AGG | - | chr7.4:39290958-39290977 | MS.gene055141:intron | 60.0% |
| !! | GGGAGACTAGTGGGTTCAGG+TGG | - | chr7.4:39290961-39290980 | MS.gene055141:intron | 60.0% |
| !! | GTTCAGGTGGCGTCGGTTCA+AGG | - | chr7.4:39290974-39290993 | MS.gene055141:intron | 60.0% |
| CCAAACGCAAGGACAGGGGG+AGG | + | chr7.4:39290908-39290927 | None:intergenic | 65.0% | |
| CCTCCCCCTGTCCTTGCGTT+TGG | - | chr7.4:39290905-39290924 | MS.gene055141:intron | 65.0% | |
| CGGCCCAAACGCAAGGACAG+GGG | + | chr7.4:39290912-39290931 | None:intergenic | 65.0% | |
| GGCCCAAACGCAAGGACAGG+GGG | + | chr7.4:39290911-39290930 | None:intergenic | 65.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr7.4 | gene | 39290268 | 39292340 | 39290268 | ID=MS.gene055141 |
| chr7.4 | mRNA | 39290268 | 39292340 | 39290268 | ID=MS.gene055141.t1;Parent=MS.gene055141 |
| chr7.4 | exon | 39292020 | 39292340 | 39292020 | ID=MS.gene055141.t1.exon1;Parent=MS.gene055141.t1 |
| chr7.4 | CDS | 39292020 | 39292340 | 39292020 | ID=cds.MS.gene055141.t1;Parent=MS.gene055141.t1 |
| chr7.4 | exon | 39290268 | 39290612 | 39290268 | ID=MS.gene055141.t1.exon2;Parent=MS.gene055141.t1 |
| chr7.4 | CDS | 39290268 | 39290612 | 39290268 | ID=cds.MS.gene055141.t1;Parent=MS.gene055141.t1 |
| Gene Sequence |
| Protein sequence |
