Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene76194.t1 | XP_013468026.1 | 95.6 | 298 | 13 | 0 | 1 | 298 | 671 | 968 | 5.30E-163 | 583.6 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene76194.t1 | A0A072VKV4 | 95.6 | 298 | 13 | 0 | 1 | 298 | 671 | 968 | 3.8e-163 | 583.6 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene052391 | MS.gene76194 | 0.816257 | 6.07E-52 | -1.69E-46 |
| MS.gene056853 | MS.gene76194 | 0.821689 | 3.53E-53 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 0 sgRNAs with CRISPR-Local
Find 119 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !!! | AGTCATAATTATTTTTATTC+TGG | + | 10762:29801-29820 | None:intergenic | 15.0% |
| !!! | GTCATAATTATTTTTATTCT+GGG | + | 10762:29800-29819 | None:intergenic | 15.0% |
| !! | CTCTAATATTGATAAGAAAA+TGG | - | 10762:30495-30514 | MS.gene76194:CDS | 20.0% |
| !!! | AGAGTTTTAAAAGAAAATGA+GGG | + | 10762:29644-29663 | None:intergenic | 20.0% |
| !!! | TAAGTTAAGTACTCTTTTAA+GGG | + | 10762:29594-29613 | None:intergenic | 20.0% |
| !!! | TAATTATTTTTATTCTGGGA+TGG | + | 10762:29796-29815 | None:intergenic | 20.0% |
| !!! | TGTTTCTTTTAGAAAAAACT+GGG | - | 10762:29161-29180 | MS.gene76194:intron | 20.0% |
| !!! | TTGTTTCTTTTAGAAAAAAC+TGG | - | 10762:29160-29179 | MS.gene76194:intron | 20.0% |
| ! | AAAATTCATGTACCTAATCA+AGG | + | 10762:29763-29782 | None:intergenic | 25.0% |
| ! | ATATGATCCAGTTAAAATCA+TGG | + | 10762:28864-28883 | None:intergenic | 25.0% |
| ! | GTTGCATACTATGAAAAATA+CGG | - | 10762:29201-29220 | MS.gene76194:intron | 25.0% |
| !! | GTAAGTTAAGTACTCTTTTA+AGG | + | 10762:29595-29614 | None:intergenic | 25.0% |
| !!! | CAGAGTTTTAAAAGAAAATG+AGG | + | 10762:29645-29664 | None:intergenic | 25.0% |
| AACAATGAAAACCATTAGCT+TGG | - | 10762:28966-28985 | MS.gene76194:CDS | 30.0% | |
| ATACTATGAAAAATACGGTG+TGG | - | 10762:29206-29225 | MS.gene76194:intron | 30.0% | |
| CTGACATGTTATTATAGAAC+AGG | + | 10762:29355-29374 | None:intergenic | 30.0% | |
| CTGTTACAACTACAAATTTG+AGG | + | 10762:29737-29756 | None:intergenic | 30.0% | |
| CTGTTCTATAATAACATGTC+AGG | - | 10762:29353-29372 | MS.gene76194:CDS | 30.0% | |
| CTTCTGTGATGTTATCAAAA+TGG | - | 10762:29134-29153 | MS.gene76194:intron | 30.0% | |
| GAATCACATCATAATTAGCA+AGG | + | 10762:30093-30112 | None:intergenic | 30.0% | |
| TAGAGTTCAACTTAAAACAG+TGG | + | 10762:29527-29546 | None:intergenic | 30.0% | |
| TATAGAACAGGTCATTTAGA+TGG | + | 10762:29343-29362 | None:intergenic | 30.0% | |
| TTGTAACAGAATCCTTGATT+AGG | - | 10762:29748-29767 | MS.gene76194:intron | 30.0% | |
| ! | ACTGATGATTGTCAGATTTT+AGG | - | 10762:30063-30082 | MS.gene76194:intron | 30.0% |
| ! | ATTAAACGCAATTCTGCTTT+TGG | + | 10762:30025-30044 | None:intergenic | 30.0% |
| ! | GTTAAAATCATGGATTACCT+TGG | + | 10762:28854-28873 | None:intergenic | 30.0% |
| ! | TCTTTGTGATGGTGATAATA+TGG | - | 10762:30174-30193 | MS.gene76194:intron | 30.0% |
| ! | TGATGGTGATAATATGGATT+TGG | - | 10762:30180-30199 | MS.gene76194:intron | 30.0% |
| ! | TGCTTTTGGATAATAAGTCT+CGG | + | 10762:30011-30030 | None:intergenic | 30.0% |
| !! | AGGTAATCCATGATTTTAAC+TGG | - | 10762:28854-28873 | MS.gene76194:CDS | 30.0% |
| !!! | ATCATTATGTGTTTTGAGGA+TGG | - | 10762:28943-28962 | MS.gene76194:CDS | 30.0% |
| !!! | ATTTTTATTCTGGGATGGAT+TGG | + | 10762:29791-29810 | None:intergenic | 30.0% |
| ACTGATCCACATAGAAAGAA+TGG | + | 10762:29892-29911 | None:intergenic | 35.0% | |
| AGCTGCAACAAATTGAAGAT+TGG | + | 10762:30155-30174 | None:intergenic | 35.0% | |
| AGTGAGATGGAACATTAACA+AGG | - | 10762:30408-30427 | MS.gene76194:intron | 35.0% | |
| ATAATGAAAAGCCGGATTCT+GGG | - | 10762:30341-30360 | MS.gene76194:CDS | 35.0% | |
| ATGCTACTCTTACCTGAAAA+CGG | + | 10762:29415-29434 | None:intergenic | 35.0% | |
| CAGACTGTAAATGATTCTCT+GGG | + | 10762:28816-28835 | None:intergenic | 35.0% | |
| CGTATTCATCTTATGCCATT+TGG | - | 10762:29064-29083 | MS.gene76194:CDS | 35.0% | |
| CTATAATAACATGTCAGGCA+TGG | - | 10762:29358-29377 | MS.gene76194:CDS | 35.0% | |
| GAATCATTTACAGTCTGGAT+TGG | - | 10762:28819-28838 | MS.gene76194:CDS | 35.0% | |
| GTGAGATGGAACATTAACAA+GGG | - | 10762:30409-30428 | MS.gene76194:intron | 35.0% | |
| TCAGCTAACAACTAAATCAG+TGG | - | 10762:29840-29859 | MS.gene76194:intron | 35.0% | |
| TGAAGCAACTAAACGAAAAC+TGG | + | 10762:29708-29727 | None:intergenic | 35.0% | |
| ! | AGGTAAGAGTAGCATTTTGT+TGG | - | 10762:29420-29439 | MS.gene76194:CDS | 35.0% |
| ! | CTGCATCATTATGTGTTTTG+AGG | - | 10762:28939-28958 | MS.gene76194:CDS | 35.0% |
| !! | ACAGTAATGTGCTGTTTAAG+TGG | + | 10762:29313-29332 | None:intergenic | 35.0% |
| !!! | TCTTTTAAAACTCTGAGACG+TGG | - | 10762:29650-29669 | MS.gene76194:intron | 35.0% |
| AAGAAACAACCTTGGTTGCA+TGG | - | 10762:29036-29055 | MS.gene76194:CDS | 40.0% | |
| ACACTGGAAAGAAACAACCT+TGG | - | 10762:29028-29047 | MS.gene76194:CDS | 40.0% | |
| ACTTAAAACAGTGGCATGCA+AGG | + | 10762:29518-29537 | None:intergenic | 40.0% | |
| ATCTTAGGAGGAAAGACTTC+GGG | + | 10762:30373-30392 | None:intergenic | 40.0% | |
| CAGCATTGCATAAAGTGAGA+TGG | - | 10762:30395-30414 | MS.gene76194:intron | 40.0% | |
| CATCTTAGGAGGAAAGACTT+CGG | + | 10762:30374-30393 | None:intergenic | 40.0% | |
| CCAGACTGTAAATGATTCTC+TGG | + | 10762:28817-28836 | None:intergenic | 40.0% | |
| CCAGAGAATCATTTACAGTC+TGG | - | 10762:28814-28833 | MS.gene76194:CDS | 40.0% | |
| CCGAAAACAGTACCTGTTAT+CGG | + | 10762:29105-29124 | None:intergenic | 40.0% | |
| CGACTTCCATTCTTTCTATG+TGG | - | 10762:29883-29902 | MS.gene76194:intron | 40.0% | |
| CTCTTTCTTGCAGCTTCAAT+GGG | + | 10762:29969-29988 | None:intergenic | 40.0% | |
| CTTAAAACAGTGGCATGCAA+GGG | + | 10762:29517-29536 | None:intergenic | 40.0% | |
| CTTTCCAGTGTATATCGTTC+CGG | + | 10762:29019-29038 | None:intergenic | 40.0% | |
| GATAATGAAAAGCCGGATTC+TGG | - | 10762:30340-30359 | MS.gene76194:CDS | 40.0% | |
| GTCCTCTCAAATTCATACCA+AGG | + | 10762:29684-29703 | None:intergenic | 40.0% | |
| TGACACTTGAACACTCCAAA+TGG | + | 10762:29082-29101 | None:intergenic | 40.0% | |
| TGCCTTGGTATGAATTTGAG+AGG | - | 10762:29679-29698 | MS.gene76194:intron | 40.0% | |
| TTAACAAGGGTAGTGAGAGA+TGG | - | 10762:30422-30441 | MS.gene76194:intron | 40.0% | |
| TTATGCAATGCTGCCATCTT+AGG | + | 10762:30388-30407 | None:intergenic | 40.0% | |
| TTTGTTGCAGCTCTTTGTGA+TGG | - | 10762:30163-30182 | MS.gene76194:intron | 40.0% | |
| ! | AAATGATTCTCTGGGAAGGT+TGG | + | 10762:28808-28827 | None:intergenic | 40.0% |
| ! | CGGCTTTTCATTATCAACTC+CGG | + | 10762:30335-30354 | None:intergenic | 40.0% |
| ! | CTGTAAATGATTCTCTGGGA+AGG | + | 10762:28812-28831 | None:intergenic | 40.0% |
| ! | GAAAACCATTAGCTTGGCAA+AGG | - | 10762:28972-28991 | MS.gene76194:CDS | 40.0% |
| ! | GGATTTGGAATCTGTATCTG+AGG | - | 10762:30195-30214 | MS.gene76194:intron | 40.0% |
| ! | GGCTTTTCATTATCAACTCC+GGG | + | 10762:30334-30353 | None:intergenic | 40.0% |
| ! | TTTCTGCTACAGCTCAGTTT+TGG | + | 10762:30253-30272 | None:intergenic | 40.0% |
| !! | ACAGCTCAGTTTTGGTCTTT+TGG | + | 10762:30245-30264 | None:intergenic | 40.0% |
| !! | CCGATAACAGGTACTGTTTT+CGG | - | 10762:29102-29121 | MS.gene76194:CDS | 40.0% |
| !! | CGGAGTTGATAATGAAAAGC+CGG | - | 10762:30333-30352 | MS.gene76194:CDS | 40.0% |
| !! | TAATGGACTTGTACGTTGTC+AGG | - | 10762:30462-30481 | MS.gene76194:CDS | 40.0% |
| !! | TTTATTCTGGGATGGATTGG+TGG | + | 10762:29788-29807 | None:intergenic | 40.0% |
| ACAGCTCTTGCTTGTAGAGA+TGG | - | 10762:30292-30311 | MS.gene76194:CDS | 45.0% | |
| AGTGAGAGATGGTTGTGCTT+TGG | - | 10762:30433-30452 | MS.gene76194:intron | 45.0% | |
| ATGTGGATCAGTGACTGAAG+AGG | - | 10762:29900-29919 | MS.gene76194:CDS | 45.0% | |
| CCATTGAAGCTGCAAGAAAG+AGG | - | 10762:29967-29986 | MS.gene76194:intron | 45.0% | |
| CCTCTTTCTTGCAGCTTCAA+TGG | + | 10762:29970-29989 | None:intergenic | 45.0% | |
| CGAAGTCTTTCCTCCTAAGA+TGG | - | 10762:30372-30391 | MS.gene76194:intron | 45.0% | |
| GGGAAAGGAGAGTTTGATAC+TGG | + | 10762:29949-29968 | None:intergenic | 45.0% | |
| GTGAGAGATGGTTGTGCTTT+GGG | - | 10762:30434-30453 | MS.gene76194:intron | 45.0% | |
| GTTCAAGTGTCACCGATAAC+AGG | - | 10762:29090-29109 | MS.gene76194:CDS | 45.0% | |
| GTTGCATATTGTGGTGCAGA+TGG | - | 10762:29380-29399 | MS.gene76194:CDS | 45.0% | |
| TACGTAGTACCATGCAACCA+AGG | + | 10762:29048-29067 | None:intergenic | 45.0% | |
| TCAGGCATGGTTGCATATTG+TGG | - | 10762:29371-29390 | MS.gene76194:CDS | 45.0% | |
| TCTTGCAGCTTCAATGGGAA+AGG | + | 10762:29964-29983 | None:intergenic | 45.0% | |
| TGAGAGATGGTTGTGCTTTG+GGG | - | 10762:30435-30454 | MS.gene76194:intron | 45.0% | |
| TGTATATCGTTCCGGTGACA+GGG | + | 10762:29011-29030 | None:intergenic | 45.0% | |
| TTCTAATACCACGCCAGAAG+AGG | + | 10762:29469-29488 | None:intergenic | 45.0% | |
| !! | CTGAGGAAGCATTGTCTTCT+CGG | - | 10762:30212-30231 | MS.gene76194:CDS | 45.0% |
| !! | TCAACTCCGGGAGTATTTGT+CGG | + | 10762:30322-30341 | None:intergenic | 45.0% |
| !! | TGAGGAAGCATTGTCTTCTC+GGG | - | 10762:30213-30232 | MS.gene76194:CDS | 45.0% |
| GAGAGATGGTTGTGCTTTGG+GGG | - | 10762:30436-30455 | MS.gene76194:intron | 50.0% | |
| GCAATTACCAGCACCTCTTC+TGG | - | 10762:29453-29472 | MS.gene76194:intron | 50.0% | |
| GTCACCGGAACGATATACAC+TGG | - | 10762:29012-29031 | MS.gene76194:CDS | 50.0% | |
| GTGTATATCGTTCCGGTGAC+AGG | + | 10762:29012-29031 | None:intergenic | 50.0% | |
| TGCAATGCTGCCATCTTAGG+AGG | + | 10762:30385-30404 | None:intergenic | 50.0% | |
| TGCAGCCTTTGCCAAGCTAA+TGG | + | 10762:28980-28999 | None:intergenic | 50.0% | |
| ! | GAGACGTGGATCTTTTGCCT+TGG | - | 10762:29664-29683 | MS.gene76194:intron | 50.0% |
| ! | TGATTCTCTGGGAAGGTTGG+AGG | + | 10762:28805-28824 | None:intergenic | 50.0% |
| !! | TGGATTGGCAGTCAAAGCCA+AGG | - | 10762:28834-28853 | MS.gene76194:CDS | 50.0% |
| !! | TTGTGCTTTGGGGGAGCTAA+TGG | - | 10762:30445-30464 | MS.gene76194:intron | 50.0% |
| AGGAGCCTCAGAGGACGAAA+TGG | + | 10762:28785-28804 | None:intergenic | 55.0% | |
| ATATCGTTCCGGTGACAGGG+AGG | + | 10762:29008-29027 | None:intergenic | 55.0% | |
| GCAAATGACCTCCCTGTCAC+CGG | - | 10762:28997-29016 | MS.gene76194:CDS | 55.0% | |
| GCAAGAGCTGTGCTTTCAGC+TGG | + | 10762:30283-30302 | None:intergenic | 55.0% | |
| TCGGGAGTAGTCCCAGAATC+CGG | + | 10762:30355-30374 | None:intergenic | 55.0% | |
| !!! | TGGTGCTGCTGTCCGTTTTC+AGG | - | 10762:29400-29419 | MS.gene76194:CDS | 55.0% |
| GAAGGTTGGAGGAGCCTCAG+AGG | + | 10762:28794-28813 | None:intergenic | 60.0% | |
| TACCAGCACCTCTTCTGGCG+TGG | - | 10762:29458-29477 | MS.gene76194:intron | 60.0% | |
| ! | GGTGCGCCGACAAATACTCC+CGG | - | 10762:30313-30332 | MS.gene76194:CDS | 60.0% |
| ! | TACCACGCCAGAAGAGGTGC+TGG | + | 10762:29463-29482 | None:intergenic | 60.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| 10762 | gene | 28778 | 30531 | 28778 | ID=MS.gene76194 |
| 10762 | mRNA | 28778 | 30531 | 28778 | ID=MS.gene76194.t1;Parent=MS.gene76194 |
| 10762 | exon | 30454 | 30531 | 30454 | ID=MS.gene76194.t1.exon1;Parent=MS.gene76194.t1 |
| 10762 | CDS | 30454 | 30531 | 30454 | ID=cds.MS.gene76194.t1;Parent=MS.gene76194.t1 |
| 10762 | exon | 30198 | 30370 | 30198 | ID=MS.gene76194.t1.exon2;Parent=MS.gene76194.t1 |
| 10762 | CDS | 30198 | 30370 | 30198 | ID=cds.MS.gene76194.t1;Parent=MS.gene76194.t1 |
| 10762 | exon | 29888 | 29934 | 29888 | ID=MS.gene76194.t1.exon3;Parent=MS.gene76194.t1 |
| 10762 | CDS | 29888 | 29934 | 29888 | ID=cds.MS.gene76194.t1;Parent=MS.gene76194.t1 |
| 10762 | exon | 29225 | 29465 | 29225 | ID=MS.gene76194.t1.exon4;Parent=MS.gene76194.t1 |
| 10762 | CDS | 29225 | 29465 | 29225 | ID=cds.MS.gene76194.t1;Parent=MS.gene76194.t1 |
| 10762 | exon | 28778 | 29136 | 28778 | ID=MS.gene76194.t1.exon5;Parent=MS.gene76194.t1 |
| 10762 | CDS | 28778 | 29136 | 28778 | ID=cds.MS.gene76194.t1;Parent=MS.gene76194.t1 |
| Gene Sequence |
| Protein sequence |
