AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene052391

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene052391.t1 MTR_7g098430 98.305 826 14 0 1 826 1 826 0.0 1675
MS.gene052391.t1 MTR_8g088050 39.753 566 322 6 158 711 18 576 1.90e-134 429
MS.gene052391.t1 MTR_8g088050 39.753 566 322 6 158 711 18 576 1.94e-134 429
MS.gene052391.t1 MTR_8g088050 39.753 566 322 6 158 711 18 576 1.96e-134 429
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene052391.t1 AT1G04080 65.057 704 224 9 109 795 37 735 0.0 914
MS.gene052391.t1 AT1G04080 65.057 704 224 9 109 795 37 735 0.0 914
MS.gene052391.t1 AT1G04080 59.527 761 227 12 109 795 37 790 0.0 866
MS.gene052391.t1 AT1G04080 65.027 549 173 7 261 795 6 549 0.0 702
MS.gene052391.t1 AT1G04080 63.916 521 169 7 289 795 1 516 0.0 652
MS.gene052391.t1 AT5G46400 41.392 546 296 9 154 687 30 563 2.18e-128 410
MS.gene052391.t1 AT5G46400 40.000 325 188 4 364 687 25 343 1.82e-66 237
MS.gene052391.t1 AT5G46400 40.000 325 188 4 364 687 25 343 1.82e-66 237
MS.gene052391.t1 AT4G24270 25.392 319 185 14 162 465 59 339 9.29e-11 66.2
MS.gene052391.t1 AT4G24270 25.392 319 185 14 162 465 59 339 9.87e-11 66.2

Find 208 sgRNAs with CRISPR-Local

Find 0 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TCATTCTGTCATTAGGCTTC+TGG 0.121133 7.4:-11958171 MS.gene052391:intron
GCTTCTCCTGCAGCCTCGGC+TGG 0.142915 7.4:+11963607 None:intergenic
GGCTATGCATTTGCTTATTC+TGG 0.172001 7.4:-11963562 MS.gene052391:CDS
TATCGATCCAATTGCTGATT+TGG 0.218895 7.4:+11960903 None:intergenic
CTTGCTACACCAGGGTTCTC+TGG 0.220827 7.4:+11957833 None:intergenic
ATTTGGATTCTCTAATATTC+TGG 0.228707 7.4:+11960920 None:intergenic
TGAGCTTGTAGTACAGAATT+AGG 0.232263 7.4:+11963511 None:intergenic
CAGAATTCCCTTTGTGTTAT+GGG 0.232733 7.4:-11961838 MS.gene052391:CDS
ATTATCTTTCGTTTCCTCTT+TGG 0.236191 7.4:-11961004 MS.gene052391:CDS
TGAAAACTGGCATAATTATC+TGG 0.271539 7.4:-11960396 MS.gene052391:CDS
CTTATACATCTCTTCTCTAA+TGG 0.271968 7.4:+11960511 None:intergenic
CACGTACGGCCTCTTAATAT+TGG 0.275797 7.4:-11960425 MS.gene052391:CDS
CCAAACTGTTCTTGCCAAAC+AGG 0.276139 7.4:-11957616 MS.gene052391:CDS
GATACAGTTGGACTTGAAAA+TGG 0.279480 7.4:-11963361 MS.gene052391:CDS
ACGTACGGCCTCTTAATATT+GGG 0.282452 7.4:-11960424 MS.gene052391:CDS
CTTCTCCTGCAGCCTCGGCT+GGG 0.286804 7.4:+11963608 None:intergenic
TTATCTTTCGTTTCCTCTTT+GGG 0.287270 7.4:-11961003 MS.gene052391:CDS
GCTGCTGCTCTGCCCAATTC+AGG 0.288615 7.4:-11963583 MS.gene052391:CDS
GCAGAATTCCCTTTGTGTTA+TGG 0.289100 7.4:-11961839 MS.gene052391:CDS
GCTGACCATTCATCTGTAAA+TGG 0.289348 7.4:-11963391 MS.gene052391:CDS
CGGCTGCAGGTTGCTGTGTC+GGG 0.291007 7.4:+11956264 None:intergenic
AATACAGCACTTGCAGGTTT+AGG 0.295664 7.4:+11960566 None:intergenic
CATTCTGTCATTAGGCTTCT+GGG 0.297685 7.4:-11958170 MS.gene052391:intron
CCTTGTGCTTGAGTCGTTGC+TGG 0.297776 7.4:+11957428 None:intergenic
GTATTCTCGATTTGTTTATT+TGG 0.319345 7.4:-11958614 MS.gene052391:intron
TGCTGTGTCGGGGCTGCAAC+AGG 0.320209 7.4:+11956275 None:intergenic
GTTCCACAAAGCACTGCTTA+TGG 0.321030 7.4:-11956797 MS.gene052391:CDS
TCCCTTTGTGTTATGGGTAT+TGG 0.321785 7.4:-11961832 MS.gene052391:CDS
AGCAAATGCATAGCCTGAAT+TGG 0.323452 7.4:+11963570 None:intergenic
TATGGGGCTTATGCTGGATA+TGG 0.331066 7.4:-11956854 MS.gene052391:CDS
TATCCAGTACTCGGGATAAT+TGG 0.333216 7.4:+11959810 None:intergenic
GCCGTTCCTCAGGCATATTA+TGG 0.342067 7.4:-11956245 MS.gene052391:CDS
TGCCTTCTCTTTGTATGAAC+AGG 0.345610 7.4:-11958695 MS.gene052391:CDS
ATCGACCTCTTTCAGAATTA+CGG 0.355782 7.4:-11960685 MS.gene052391:CDS
CCTAACCAATGGCCTAACTA+TGG 0.364535 7.4:-11957470 MS.gene052391:CDS
GGCCATTGGTTAGGTCCATT+TGG 0.367818 7.4:+11957479 None:intergenic
TTCCACAAAGCACTGCTTAT+GGG 0.376637 7.4:-11956796 MS.gene052391:CDS
CCTGTTTGGCAAGAACAGTT+TGG 0.377400 7.4:+11957616 None:intergenic
TACACCAGGGTTCTCTGGGT+TGG 0.381425 7.4:+11957838 None:intergenic
TATGGAAGCTAGGGAAAGTA+TGG 0.382672 7.4:-11959773 MS.gene052391:CDS
TGCTGCTGGGTGTAGCCAGC+AGG 0.383369 7.4:+11957395 None:intergenic
TCTGTTAATACAGCACTTGC+AGG 0.384276 7.4:+11960560 None:intergenic
ATGAGTACATGCAGCAAGAC+TGG 0.385484 7.4:-11960965 MS.gene052391:CDS
CAATGTCAACTCGCTTTGGC+TGG 0.386893 7.4:+11957897 None:intergenic
AATAATCAGGCATGCCAATA+TGG 0.389365 7.4:-11958826 MS.gene052391:CDS
CCTGCAGCCTCGGCTGGGGC+AGG 0.390779 7.4:+11963613 None:intergenic
GAGATGTTCAATCGATCAAA+AGG 0.399956 7.4:-11957655 MS.gene052391:CDS
AGCAGCTTCTCCTGCAGCCT+CGG 0.403142 7.4:+11963603 None:intergenic
GATGAAGCTGCTGCAGTGGC+AGG 0.405134 7.4:-11960656 MS.gene052391:CDS
ACACCAGGGTTCTCTGGGTT+GGG 0.411863 7.4:+11957839 None:intergenic
TGAGGCTGTACTCCATAGTT+AGG 0.412997 7.4:+11957458 None:intergenic
ATACAGGCTGCCGCTTCCTA+TGG 0.418623 7.4:-11956872 MS.gene052391:intron
AACTCCGACAAGCCCCTGTT+TGG 0.419402 7.4:+11957602 None:intergenic
TTCTCCTGCAGCCTCGGCTG+GGG 0.420645 7.4:+11963609 None:intergenic
TTGCGCTCGGTTCAAGGAGC+AGG 0.422648 7.4:-11958922 MS.gene052391:CDS
GGAGAATTATCTGCACCATC+AGG 0.426487 7.4:+11960587 None:intergenic
AATGTCAACTCGCTTTGGCT+GGG 0.427195 7.4:+11957898 None:intergenic
TATTACCTACGAACTGTGTC+TGG 0.428305 7.4:+11961668 None:intergenic
CATGATATTGCAGGGGAAGT+TGG 0.428308 7.4:-11958722 MS.gene052391:intron
ACGGCTGCAGGTTGCTGTGT+CGG 0.430705 7.4:+11956263 None:intergenic
TTCACCTGTGCAGGATATGC+AGG 0.431301 7.4:+11956764 None:intergenic
TAAATGGTGTTGCAGCTTCA+AGG 0.434451 7.4:-11960720 MS.gene052391:intron
TACAGGCTGCCGCTTCCTAT+GGG 0.435003 7.4:-11956871 MS.gene052391:intron
CCTGCCCCAGCCGAGGCTGC+AGG 0.435556 7.4:-11963613 MS.gene052391:CDS
TCAAGCTCCCCAATATTAAG+AGG 0.435934 7.4:+11960416 None:intergenic
TACCAATCGATGTTCCATAT+TGG 0.436137 7.4:+11958812 None:intergenic
GATGGGAATGCTTATACTGC+TGG 0.441620 7.4:-11963538 MS.gene052391:CDS
CCGTTCCTCAGGCATATTAT+GGG 0.443253 7.4:-11956244 MS.gene052391:CDS
AATTGGATCGATATTTCAAC+AGG 0.444059 7.4:-11960893 MS.gene052391:intron
AAAACCAATTTACCTGCTGC+TGG 0.444538 7.4:+11957381 None:intergenic
GTCTTCTTGAAGCAATAATC+AGG 0.448175 7.4:-11958839 MS.gene052391:CDS
AGAGAATCCAAATCAGCAAT+TGG 0.448924 7.4:-11960910 MS.gene052391:CDS
TTGCTACACCAGGGTTCTCT+GGG 0.452718 7.4:+11957834 None:intergenic
GTTCACACTGAAATTTCTCC+AGG 0.453659 7.4:-11958861 MS.gene052391:CDS
TCAAATCCTGTTCCTGAGGT+TGG 0.455191 7.4:-11963646 MS.gene052391:CDS
CATTGACTTTCTAGAGTCAC+TGG 0.456681 7.4:-11957879 MS.gene052391:CDS
GCCCCATAAGCAGTGCTTTG+TGG 0.457630 7.4:+11956794 None:intergenic
ATATTGGGGAGCTTGAAAAC+TGG 0.459736 7.4:-11960409 MS.gene052391:CDS
GATATGCAGCCACTACAGAT+TGG 0.459844 7.4:+11957501 None:intergenic
GCTGACCATGAGGCGCGCCT+TGG 0.460933 7.4:-11961800 MS.gene052391:CDS
GCTTCCTATGGGGCTTATGC+TGG 0.462340 7.4:-11956860 MS.gene052391:CDS
GCATTTGCTTATTCTGGTGA+TGG 0.462692 7.4:-11963556 MS.gene052391:CDS
TCTGGTTGGAAAGGAAGCTC+TGG 0.463748 7.4:-11963431 MS.gene052391:CDS
CTTGCCAAACAGGGGCTTGT+CGG 0.464209 7.4:-11957606 MS.gene052391:CDS
GAGTTCGATTCTAAGATCAT+TGG 0.466817 7.4:-11960479 MS.gene052391:CDS
CACTTCCATTTACAGATGAA+TGG 0.468952 7.4:+11963386 None:intergenic
CCATAGTTAGGCCATTGGTT+AGG 0.469295 7.4:+11957470 None:intergenic
TGCTGGCTACACCCAGCAGC+AGG 0.471209 7.4:-11957393 MS.gene052391:intron
GTTTATGAACGAGCTGTTCA+AGG 0.479117 7.4:-11961755 MS.gene052391:CDS
GAACGAGGATTAGCTTATGT+TGG 0.479715 7.4:-11961032 MS.gene052391:CDS
AGCTTCCTTTCCAACCAGAC+TGG 0.482155 7.4:+11963435 None:intergenic
TGCAAATAATGTTCTCGCTC+GGG 0.483808 7.4:-11959746 MS.gene052391:CDS
GCAAATGCATAGCCTGAATT+GGG 0.492299 7.4:+11963571 None:intergenic
ATGTCAACTCGCTTTGGCTG+GGG 0.492568 7.4:+11957899 None:intergenic
AATGAGTCAAAGCAAGCTGC+TGG 0.493532 7.4:-11963475 MS.gene052391:CDS
TTAGCACCAACCTCAGGAAC+AGG 0.493830 7.4:+11963640 None:intergenic
GTGCCAATTATCCCGAGTAC+TGG 0.494096 7.4:-11959813 MS.gene052391:CDS
AAGTCAATGTCAACTCGCTT+TGG 0.496185 7.4:+11957893 None:intergenic
CAAGGACAACAATGGCCTGC+TGG 0.496647 7.4:-11957410 MS.gene052391:CDS
GCCTAATGAGGATTCCAGTC+TGG 0.497327 7.4:-11963449 MS.gene052391:CDS
ATTAACAGAAGCAGAAGAGT+TGG 0.497386 7.4:-11960546 MS.gene052391:CDS
GGAACTGATGCAGGCAATTG+TGG 0.498199 7.4:+11956815 None:intergenic
TCCAATACCCATAACACAAA+GGG 0.499353 7.4:+11961831 None:intergenic
CAGGCCATTGCCATTGAGAA+AGG 0.499761 7.4:-11958676 MS.gene052391:CDS
TATGCCTGAGGAACGGCTGC+AGG 0.506298 7.4:+11956251 None:intergenic
GCAACCTGCAGCCGTTCCTC+AGG 0.509331 7.4:-11956255 MS.gene052391:CDS
GGCTTCTGGGAATTCCGAAA+AGG 0.517187 7.4:-11958157 MS.gene052391:CDS
GGTACTGACCAAGGTGTTGA+AGG 0.517289 7.4:-11960620 MS.gene052391:CDS
GAGCAGGCTGGAGATATTGT+TGG 0.520258 7.4:-11958906 MS.gene052391:CDS
AAGAGGCCGTACGTGAAAGT+AGG 0.521077 7.4:+11960433 None:intergenic
CAGAGCCAAGGCGCGCCTCA+TGG 0.522186 7.4:+11961795 None:intergenic
ATATGCAGCCACTACAGATT+GGG 0.522584 7.4:+11957502 None:intergenic
CGAGACAGAGAAGGTGGCAG+AGG 0.524685 7.4:-11962329 MS.gene052391:intron
CATTTGCTTATTCTGGTGAT+GGG 0.527079 7.4:-11963555 MS.gene052391:CDS
TGCCAATATGGAACATCGAT+TGG 0.531852 7.4:-11958814 MS.gene052391:intron
TTGCAAATAATGTTCTCGCT+CGG 0.532036 7.4:-11959747 MS.gene052391:CDS
TCCTCCTGCATATCCTGCAC+AGG 0.532285 7.4:-11956768 MS.gene052391:intron
GTGTTGCAGCTTCAAGGAGT+TGG 0.532516 7.4:-11960714 MS.gene052391:intron
GTCGTTGCTGGCCAAGTTTG+AGG 0.533522 7.4:+11957440 None:intergenic
ATGGAGTACAGCCTCAAACT+TGG 0.534256 7.4:-11957451 MS.gene052391:CDS
ATGGGAATGCTTATACTGCT+GGG 0.536210 7.4:-11963537 MS.gene052391:CDS
ACCAGACTGGAATCCTCATT+AGG 0.536266 7.4:+11963448 None:intergenic
CGCTTCAAATCCTGTTCCTG+AGG 0.536806 7.4:-11963650 MS.gene052391:CDS
AACATAGCGTATCCAGTACT+CGG 0.538429 7.4:+11959801 None:intergenic
TCTGTTGCACTTGCTACACC+AGG 0.538737 7.4:+11957824 None:intergenic
TCAATGTATTTATCCCAAAG+AGG 0.538798 7.4:+11960990 None:intergenic
ATATTCGCTATAAGCACCTA+TGG 0.541316 7.4:-11961695 MS.gene052391:CDS
GCTCGGTTCAAGGAGCAGGC+TGG 0.541555 7.4:-11958918 MS.gene052391:CDS
CAAACTGTTCTTGCCAAACA+GGG 0.544913 7.4:-11957615 MS.gene052391:CDS
AGCATAAGCCCCATAGGAAG+CGG 0.546523 7.4:+11956862 None:intergenic
GCTGTCCGTAATTCTGAAAG+AGG 0.548224 7.4:+11960680 None:intergenic
GGCTGCAGGTTGCTGTGTCG+GGG 0.548629 7.4:+11956265 None:intergenic
GTGGCAGGTGTTGTTTCAGA+GGG 0.550122 7.4:-11960641 MS.gene052391:CDS
GTACTCCATAGTTAGGCCAT+TGG 0.550599 7.4:+11957465 None:intergenic
AATGCATCAGAGAATGTTGA+TGG 0.550820 7.4:-11963337 MS.gene052391:CDS
TCCACAAAGCACTGCTTATG+GGG 0.553188 7.4:-11956795 MS.gene052391:CDS
AGTGGCAGGTGTTGTTTCAG+AGG 0.553746 7.4:-11960642 MS.gene052391:CDS
GTAGTGGCTGCATATCCAAA+TGG 0.555000 7.4:-11957494 MS.gene052391:CDS
GTGCTTTGTGGAACTGATGC+AGG 0.555027 7.4:+11956806 None:intergenic
AAACAACTCACAGATGCTTA+CGG 0.557128 7.4:-11963301 MS.gene052391:intron
AGCTGATGAAGCTGCTGCAG+TGG 0.558284 7.4:-11960660 MS.gene052391:CDS
GGTGTAAATCATTGCAAGGC+GGG 0.558474 7.4:+11960941 None:intergenic
GGGGCTTATGCTGGATATGG+AGG 0.560147 7.4:-11956851 MS.gene052391:CDS
AACTGTGTCTGGATCTCCAT+AGG 0.560352 7.4:+11961679 None:intergenic
GGAACATTAGCACCAACCTC+AGG 0.561128 7.4:+11963634 None:intergenic
TTTATGAACGAGCTGTTCAA+GGG 0.561629 7.4:-11961754 MS.gene052391:CDS
ATTATTGCTTCAAGAAGACC+TGG 0.562102 7.4:+11958843 None:intergenic
AATGAGGATTCCAGTCTGGT+TGG 0.562814 7.4:-11963445 MS.gene052391:CDS
AGAGGCCGTACGTGAAAGTA+GGG 0.564513 7.4:+11960434 None:intergenic
GGAAGCTCTGGACTCTGCTA+TGG 0.564721 7.4:-11963419 MS.gene052391:CDS
AGATGTTCAATCGATCAAAA+GGG 0.566435 7.4:-11957654 MS.gene052391:CDS
GGATTCCAGTCTGGTTGGAA+AGG 0.569228 7.4:-11963440 MS.gene052391:CDS
TTTGATTGACGAGACAGAGA+AGG 0.570064 7.4:-11962338 MS.gene052391:CDS
AAACCAATTTACCTGCTGCT+GGG 0.571184 7.4:+11957382 None:intergenic
TCAATCGATCAAAAGGGTTG+AGG 0.572118 7.4:-11957648 MS.gene052391:CDS
GATATTGCAGGGGAAGTTGG+AGG 0.576699 7.4:-11958719 MS.gene052391:intron
TAATGTTCCTGCCCCAGCCG+AGG 0.579939 7.4:-11963620 MS.gene052391:CDS
AAGAGAAGGCGACTTGAGCA+AGG 0.580408 7.4:-11960363 MS.gene052391:intron
TGCAGGATATGCAGGAGGAT+AGG 0.581110 7.4:+11956772 None:intergenic
TTCTGGTGTAAATCATTGCA+AGG 0.581222 7.4:+11960937 None:intergenic
GAATACTGAGCAAACAACAT+TGG 0.582577 7.4:+11958631 None:intergenic
GAACTGATGCAGGCAATTGT+GGG 0.582647 7.4:+11956816 None:intergenic
AGCTGCTGGAGTGCCTAATG+AGG 0.586064 7.4:-11963461 MS.gene052391:CDS
TGCTGCTGAGACGTATCCAT+CGG 0.586175 7.4:-11963689 MS.gene052391:CDS
AATGGAAGTGTTGATACAGT+TGG 0.591174 7.4:-11963373 MS.gene052391:CDS
GCAGCCACTACAGATTGGGC+AGG 0.591823 7.4:+11957506 None:intergenic
CGTACGGCCTCTTAATATTG+GGG 0.592807 7.4:-11960423 MS.gene052391:CDS
CCCATAATATGCCTGAGGAA+CGG 0.592953 7.4:+11956244 None:intergenic
TGAGTACATGCAGCAAGACT+GGG 0.596076 7.4:-11960964 MS.gene052391:CDS
CAACTTTATCAGCAGAGCCA+AGG 0.596207 7.4:+11961783 None:intergenic
TTACATATTCAGTGGACATG+TGG 0.600677 7.4:-11961730 MS.gene052391:CDS
ATATCCAGCATAAGCCCCAT+AGG 0.601936 7.4:+11956856 None:intergenic
ACTGTCAAAGCCACTACTTG+AGG 0.604117 7.4:-11958097 MS.gene052391:intron
AGCAAGTGCAACAGAGCGCG+AGG 0.605392 7.4:-11957816 MS.gene052391:CDS
TCAAGGGGTTACATATTCAG+TGG 0.607137 7.4:-11961738 MS.gene052391:CDS
ATCACCTGCCCAATCTGTAG+TGG 0.610772 7.4:-11957510 MS.gene052391:CDS
TTCCAATACCCATAACACAA+AGG 0.612695 7.4:+11961830 None:intergenic
AACACAAAGGGAATTCTGCT+AGG 0.613157 7.4:+11961843 None:intergenic
TATGGGAGATAGTGAAACAA+TGG 0.615550 7.4:-11963725 MS.gene052391:CDS
TGACCAAGGTGTTGAAGGAG+AGG 0.619456 7.4:-11960615 MS.gene052391:CDS
GTGTTGTTGTAAGCATCCGA+TGG 0.622239 7.4:+11963673 None:intergenic
ACATAGCGTATCCAGTACTC+GGG 0.622860 7.4:+11959802 None:intergenic
ATCCAAATGGACCTAACCAA+TGG 0.623577 7.4:-11957481 MS.gene052391:CDS
ATCAGAGAATGTTGATGGGT+CGG 0.627186 7.4:-11963332 MS.gene052391:CDS
TTGAGACAAACCTCAAGTAG+TGG 0.627545 7.4:+11958087 None:intergenic
CTGCTGAAGAAGATCGATTG+TGG 0.627715 7.4:-11962405 MS.gene052391:CDS
GGCCTGTTCATACAAAGAGA+AGG 0.629259 7.4:+11958693 None:intergenic
GATTGACGAGACAGAGAAGG+TGG 0.630394 7.4:-11962335 MS.gene052391:CDS
ACAGGCTGCCGCTTCCTATG+GGG 0.637522 7.4:-11956870 MS.gene052391:intron
GTACTCGGGATAATTGGCAC+AGG 0.638207 7.4:+11959816 None:intergenic
CTCAAGCACAAGGACAACAA+TGG 0.638781 7.4:-11957418 MS.gene052391:CDS
GTTTCAGAGGGTACTGACCA+AGG 0.640522 7.4:-11960629 MS.gene052391:CDS
GAAGGAGAGGTTCATCCTGA+TGG 0.646457 7.4:-11960602 MS.gene052391:CDS
GAACCTCTCCTTCAACACCT+TGG 0.647061 7.4:+11960612 None:intergenic
ATGCATCAGAGAATGTTGAT+GGG 0.647426 7.4:-11963336 MS.gene052391:CDS
CTGTTGCACTTGCTACACCA+GGG 0.651331 7.4:+11957825 None:intergenic
ACCTGTGCAGGATATGCAGG+AGG 0.651957 7.4:+11956767 None:intergenic
AAACTGTTCTTGCCAAACAG+GGG 0.652207 7.4:-11957614 MS.gene052391:CDS
GGAGGCCCTACTTTCACGTA+CGG 0.652392 7.4:-11960439 MS.gene052391:CDS
CTCAGGAACAGGATTTGAAG+CGG 0.653630 7.4:+11963651 None:intergenic
ACACCCAACCCAGAGAACCC+TGG 0.662263 7.4:-11957842 MS.gene052391:CDS
TTATGAACGAGCTGTTCAAG+GGG 0.665698 7.4:-11961753 MS.gene052391:CDS
CCAGCAACGACTCAAGCACA+AGG 0.666384 7.4:-11957428 MS.gene052391:CDS
GAGATCCAGACACAGTTCGT+AGG 0.679080 7.4:-11961673 MS.gene052391:intron
TGGGAATGCTTATACTGCTG+GGG 0.686552 7.4:-11963536 MS.gene052391:CDS
GAAGAAATATGCTGACCATG+AGG 0.691106 7.4:-11961810 MS.gene052391:CDS
TAATTCCCATAATATGCCTG+AGG 0.692350 7.4:+11956239 None:intergenic
TGGTGTAAATCATTGCAAGG+CGG 0.722048 7.4:+11960940 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content


Chromosome Type Strat End Strand Name
chr7.4 gene 11956232 11963746 11956232 ID=MS.gene052391
chr7.4 mRNA 11956232 11963746 11956232 ID=MS.gene052391.t1;Parent=MS.gene052391
chr7.4 exon 11963302 11963746 11963302 ID=MS.gene052391.t1.exon1;Parent=MS.gene052391.t1
chr7.4 CDS 11963302 11963746 11963302 ID=cds.MS.gene052391.t1;Parent=MS.gene052391.t1
chr7.4 exon 11962330 11962436 11962330 ID=MS.gene052391.t1.exon2;Parent=MS.gene052391.t1
chr7.4 CDS 11962330 11962436 11962330 ID=cds.MS.gene052391.t1;Parent=MS.gene052391.t1
chr7.4 exon 11961674 11961903 11961674 ID=MS.gene052391.t1.exon3;Parent=MS.gene052391.t1
chr7.4 CDS 11961674 11961903 11961674 ID=cds.MS.gene052391.t1;Parent=MS.gene052391.t1
chr7.4 exon 11960894 11961061 11960894 ID=MS.gene052391.t1.exon4;Parent=MS.gene052391.t1
chr7.4 CDS 11960894 11961061 11960894 ID=cds.MS.gene052391.t1;Parent=MS.gene052391.t1
chr7.4 exon 11960364 11960727 11960364 ID=MS.gene052391.t1.exon5;Parent=MS.gene052391.t1
chr7.4 CDS 11960364 11960727 11960364 ID=cds.MS.gene052391.t1;Parent=MS.gene052391.t1
chr7.4 exon 11959726 11959869 11959726 ID=MS.gene052391.t1.exon6;Parent=MS.gene052391.t1
chr7.4 CDS 11959726 11959869 11959726 ID=cds.MS.gene052391.t1;Parent=MS.gene052391.t1
chr7.4 exon 11958815 11958967 11958815 ID=MS.gene052391.t1.exon7;Parent=MS.gene052391.t1
chr7.4 CDS 11958815 11958967 11958815 ID=cds.MS.gene052391.t1;Parent=MS.gene052391.t1
chr7.4 exon 11958615 11958731 11958615 ID=MS.gene052391.t1.exon8;Parent=MS.gene052391.t1
chr7.4 CDS 11958615 11958731 11958615 ID=cds.MS.gene052391.t1;Parent=MS.gene052391.t1
chr7.4 exon 11958098 11958178 11958098 ID=MS.gene052391.t1.exon9;Parent=MS.gene052391.t1
chr7.4 CDS 11958098 11958178 11958098 ID=cds.MS.gene052391.t1;Parent=MS.gene052391.t1
chr7.4 exon 11957793 11957948 11957793 ID=MS.gene052391.t1.exon10;Parent=MS.gene052391.t1
chr7.4 CDS 11957793 11957948 11957793 ID=cds.MS.gene052391.t1;Parent=MS.gene052391.t1
chr7.4 exon 11957394 11957693 11957394 ID=MS.gene052391.t1.exon11;Parent=MS.gene052391.t1
chr7.4 CDS 11957394 11957693 11957394 ID=cds.MS.gene052391.t1;Parent=MS.gene052391.t1
chr7.4 exon 11956769 11956888 11956769 ID=MS.gene052391.t1.exon12;Parent=MS.gene052391.t1
chr7.4 CDS 11956769 11956888 11956769 ID=cds.MS.gene052391.t1;Parent=MS.gene052391.t1
chr7.4 exon 11956232 11956327 11956232 ID=MS.gene052391.t1.exon13;Parent=MS.gene052391.t1
chr7.4 CDS 11956232 11956327 11956232 ID=cds.MS.gene052391.t1;Parent=MS.gene052391.t1
Gene Sequence

>MS.gene052391

ATGGGAGATAGTGAAACAATGGTTGCAGAAGCATCTGCTGCTGAGACGTATCCATCGGATGCTTACAACAACACCGCTTCAAATCCTGTTCCTGAGGTTGGTGCTAATGTTCCTGCCCCAGCCGAGGCTGCAGGAGAAGCTGCTGCTGCTCTGCCCAATTCAGGCTATGCATTTGCTTATTCTGGTGATGGGAATGCTTATACTGCTGGGGATCCTAATTCTGTACTACAAGCTCAATTTAGTGCAACAAATGAGTCAAAGCAAGCTGCTGGAGTGCCTAATGAGGATTCCAGTCTGGTTGGAAAGGAAGCTCTGGACTCTGCTATGGTCTCTGCTGACCATTCATCTGTAAATGGAAGTGTTGATACAGTTGGACTTGAAAATGGAAATGCATCAGAGAATGTTGATGGGTCGGCTGATGAGAAACAACTCACAGATGCTTACGGTATGTATATGCCTTCTCTGTCTGTTATGTTAGCATACTCTTCGGGAAAAGTGAAGTTATTTCGTTTTTATTGTTATTGGAGTTTTGTTAATAAAAAAATCGGAGTTCAATGTTAAGGGTATGTATAGAGCTTGCAAGTAATATAATTAACCAATGATGGAGTCAAGGACTTAAGATTCAAGTGATTTATTATTAACCAGCAAATAGTTCATTTAGGCCTTGTAGTTCATGTAAGCTTGTAAGTAATACAATTGCATATCAGTGTTGAGAACTAATGAATTTTCACCAATATTCATGAATGGAAATAAGAAAATTTTAATCGCTCATAGTAACAATAGTGTTGTCAATTGTGGATAACGGATAAAACAGTAGCGGTTTGTTAAAATTCCGCTACGCTATAGCGGTATAGTGGTTGCTGTTTAGCAACACTGAGTAAGAAGCTTTATTTTTTCATGCATTGGAGGTGGCGTTTAAATCTACTTCCATTTGTTTTTGTCTCAAGTGACTTTGAAATTTTTCCAGGCCTAGTTTGAATTGGATCTTGACCATATTGATATAATAACTAGGTTTATTAGGTGTATAAATACGTTCATACATTTACATTTTTTTTCCATTTTATTGGTTTACCAGTGCAACAAGATACCATGACTTTGGTTTGTAATTTGATGATCACATATTCTAAGCCACTTCTGCGATTTTTGGATGTCTTCTATTTTACTGAGTTGGCTGATTCAGGCCTAAACATACACGTCTTATCTCCATACGTCTTGTTTTTAATATTATCTTGTTATTAATTATTCACAAGCATATTGCTGTTCAACCAAATTATGGTTTTGCTGCTAATCATTTATCTCACGTTTAACAGCTGCGTTGTCTGCTGAAGAAGATCGATTGTGGAATATTGTAACAGCTAATTCTTCTGATTTTACTGCTTGGACTTCTTTGATTGACGAGACAGAGAAGGTGGCAGAGGTATGATATGTTGACCCAATTTTTAAGACGTGAATTACATGTCGAGGAATCAAAATAAATGACTGATCTCGATGTTTTCATATTAGTTAGATATATGACATGCATGCATTATGATTCAGTATACTACGTGTTTAATGAGATGGATAATTTATATTGTTAAATACTTAAACCTGCATCTGTTTAACGGGGTGGTCTTGATTTAGAATGTCTCTAATGTACACACAAGTTATGTGGGCGATTTCTCTGATTCTGTCTGTCGGTATATTATTTTTTAACCTGCTAGTATATTATTTTTTCCTTCTTTTGTCCCTTATTACGAGATACTTGTAGTTATAGGTTTAGTGGCAAACTAATGTGATGTATTTGATTTTATTATTGATTTATTTTATGACTCATGGTTCTCATATAATGATTTTTTTATTGAAGAACAATATTTTGAAGATGCGTAGAGTCTATGATGCTTTCCTAGCAGAATTCCCTTTGTGTTATGGGTATTGGAAGAAATATGCTGACCATGAGGCGCGCCTTGGCTCTGCTGATAAAGTTGTTGAAGTTTATGAACGAGCTGTTCAAGGGGTTACATATTCAGTGGACATGTGGTTGCATTATTGCATATTCGCTATAAGCACCTATGGAGATCCAGACACAGTTCGTAGGTAATAGAGGCTTATTTACATGTTACCTTATTATTTTCTATGATCGGATTTTCGATTTACTAGGCTCAGCTGTTTCTTGCTTGCTTTCTAACCTGATTTCAGAGACCGTGCAACAGTACCATCTCCTTTTTATGGCGGAAAAGCTTTGATGGGATTGCATGTCAGAATGTTTTCCATTCTGATGTTTAAATCCTTGGTTTTGATTCCACTATGTATAGTAGTTGAAATTTTGGGTCCATTTTCCCCTATAAACTTTTCAATTTGATTTCAAACTTCATAGTTGTGTGGAAGGGTTCTATTTTCCCCCCCTTTCCTGTTGAAATGGTTAGCTGTTTTTATCTTTGACACTATGAGGTGTATCAATTTGCAAATTGCTGCTGGGAAGATCTCGTCTGGTTAAGGGCTAGATGGCCTCTATGACTGCTCCATTTCTCGGACATTGTATGATTTTTTGCAGGAAGGTAAATGCAGCGTTTGGATGTCTTATATGATGTTACGAGTGATTTGTTTGTTACCTTAGAACACTAGTGCTGCTGTTTCCCATACATGGATGTGCATCAGTAAATGGATGCTTTAAGTTACAGATTAATCAAGACTGCTTGCTTATTTCAGGCTTTTTGAACGAGGATTAGCTTATGTTGGAACAGATTATCTTTCGTTTCCTCTTTGGGATAAATACATTGAATATGAGTACATGCAGCAAGACTGGGCCCGCCTTGCAATGATTTACACCAGAATATTAGAGAATCCAAATCAGCAATTGGATCGATATTTCAACAGGTAATTACTTGTTTTAATTTAATATTATCATTCCACTTCATTTGTTTCCAAAAAAGAAACACAATTACTATTTTTTGTTTTATATTTCTACATAATATCCCCTTCTACTATCTCCTCTCTATTTTCCCCGAACTAAATTACTTCAACGCTGTAAATGGTGTTGCAGCTTCAAGGAGTTGGCTAGTAATCGACCTCTTTCAGAATTACGGACAGCTGATGAAGCTGCTGCAGTGGCAGGTGTTGTTTCAGAGGGTACTGACCAAGGTGTTGAAGGAGAGGTTCATCCTGATGGTGCAGATAATTCTCCTAAACCTGCAAGTGCTGTATTAACAGAAGCAGAAGAGTTGGAGAAGTATATCGCCATTAGAGAAGAGATGTATAAGAAAGCTAAAGAGTTCGATTCTAAGATCATTGGTTTTGAAACAACTATCAGGAGGCCCTACTTTCACGTACGGCCTCTTAATATTGGGGAGCTTGAAAACTGGCATAATTATCTGGATTTTATAGAAAGAGAAGGCGACTTGAGCAAGGTAACCAAACTGCTTTCTTATAAGTGCACATTCTGTTCTAGTTTTTCCTGTTATAGATTGACTTACAGTAAGTAATATCTGCTGTATCATATGTCTTATAAGTGCACATCCTATTCTGTTGGATGAAGAAACTTTTTAATCGGGAATTGAACTGAGACTAAAGCATGGGCCTACAAATTGGTTTCTTGAGGCTAAGTACTTCAAACCCTAATTTGTCAGTGGACATTCTAGGTTTGTCATGTGTTTGCAAAAGTTTATTTTTAAAAGTGTGAATGTTATGAATGCAGGTATCTTCTAATCTTCATAATCACCTGTCTCAGGCAAGGAGGGTCCCTCTTTTCATATACGAGGGGCCTCTCTGTCCCGCCACGGAGTATTTCTAATGCTTTGAATTTTCTCTTTATGCTTCTCTTTCTTGATTTTTTTGCTTGCATTTACATTGTTGGACAATTTTTATGATGTGTTCTTTTCTTGTTTTGTTGTTTGTGGATCAGATTGTCAAGTTGTATGAGAGATGTGTCATTGCCTGTGCCAATTATCCCGAGTACTGGATACGCTATGTTTTGTGTATGGAAGCTAGGGAAAGTATGGATCTTGCAAATAATGTTCTCGCTCGGGCGAGCCAAGTTTTTGTCAAGGTAATTTCTTCCAAATGTCAAAGCTGTAGTTTTGGCAGCCATTTGCTGCTTTTTAGTGTTGTATTCATCTGTCCTGTTTTTAAGCTAAGTGAATTGGCAAGAAACACACATATATAATGGACTACTAGCCATAGGATAATAGTTCATGAAACTTGATATAGTGTGTCAATACACCATAGGTTGGTTTTTTGTTTACCTGATTGTAAATGGTGAAGGGGCATCTTATATTTTTGAGCTTTATGTTTATATGATTTCTTTAGTTTGGAGGTAAATGAAAGTTCAATTGTCTTTTATTTCCCCATCGGGGCCTTCGTTTAGCCGAGAATAGAAATCTTATGTACACCGCGTTGCCAATGACACATTTCGTATAAGTGAAGGCGTATAAATGATTTTTTTTTTTTATATCGCCTGTCTTACACAAGACATATGATCCCTTTGAGGTTGTGACTAGCTAGTGATTCAATTACTCATTTCCTTTTCATGGAGGTATTGGTATTATGTCGTAGATCAGTTATCCTGAAAGCCTAAACTGCCAGGTCACATATACGAAGAGTATTTTCATATCACTGGCAAGCTTTTTGTTGTGGCCCGTCAGTGAATGGTGTTTATTATATGTGCAATTTAGTCTTGATTTATAAAAAGCAATGTTCATGTATACTTTGTTTATAAGCATAATTAGCTCAATTGCATTTCTTGTGTGAGAGGCGTTTCCCTTTGTTTTGTTGCTGCTTTATCATTTTTTGCTTGTCATAATGCAGAGACAACCCGAGATACATCTTTTTTGCGCTCGGTTCAAGGAGCAGGCTGGAGATATTGTTGGCGCTAGAGCTGCGTATCAACTTGTTCACACTGAAATTTCTCCAGGTCTTCTTGAAGCAATAATCAGGCATGCCAATATGGAACATCGATTGGTATGCTGACTTACAAAAGCGTTAATAAACCGTGACTCAATTTTTGCTTAAATTTACAGCTTTTCCATTCCATGATATTGCAGGGGAAGTTGGAGGATGCCTTCTCTTTGTATGAACAGGCCATTGCCATTGAGAAAGGAAAAGAACATTCACAAACACTGCCAATGTTGTTTGCTCAGTATTCTCGATTTGTTTATTTGGTAGGTTTGGCAACATATTTGTATTCTTCGCTTGTTATTCAGTCTTGTTTTGTTTCAGACATTTCCGCATTTGGCTATAGAACATGAAATAGGGACAGTTCTCTTAATTAACATTAGCTACTGTTTTTGTTGACCTGTCTGGATCTGGCTAAATGATGATTATTTCTGCGAGCCTCTCATTTATCCCAATAAATAGAGATGATTCTGGCTTTATATTATTCAACAATTTTTATATTATTTAACAATTTTCTAGCTTTATGCTTCAGTTGCTTTACTCGTTTGTGTGAAAATTGTCTAGTCTTGTTTTTGAAATATTCTTTCTCATTCTGTCATTAGGCTTCTGGGAATTATAGTTTTATGCTTCAGTTGCTATGCTTGTTTGTGTGAAAGTTGTCTTGTCTTGTTTTTGAAATATTCTTTCTCATTCTGTCATTAGGCTTCTGGGAATTCCGAAAAGGCTAGAGAGATTTTAGTTGGGGGACTTGAGAATGCTTCACTGTCAAAGCCACTACTTGAGGTTTGTCTCAACTTTCACTTTCTATTGATACTTAACTAATATTATGCACTTGGCAATTTTTCTCTTCGTCTTTCCAAATGTTTTTCATATCTAACGAGATTATTCAACTTCATCTCTAACGAGAGTATTTAACTTCATCTCTTACACAGGCTTTATTGCATTTTGAGGCTATTCAACCCCAGCCAAAGCGAGTTGACATTGACTTTCTAGAGTCACTGGTTGTAAAATTCATAACACCCAACCCAGAGAACCCTGGTGTAGCAAGTGCAACAGAGCGCGAGGAGCTTTCTAACATTTTTCTGGAGGTGATTTCTTATGAGGAGGATGTTCAATATTATATTTTATTAGTTTCCTTTAGTTAGCAGTATTATCAAGTTCTTACAAATTTTCTGTTGTTTTTTCAGTTTTTAAATCTCTTTGGAGATGTTCAATCGATCAAAAGGGTTGAGGATAGACATGCCAAACTGTTCTTGCCAAACAGGGGCTTGTCGGAGTTGAAAAAACGTCATGCAGAGGATTTCTTAGCCTCAGATAAAACAAAAGTGTCAAGAGCTTATTCTGCTCAATCACCTGCCCAATCTGTAGTGGCTGCATATCCAAATGGACCTAACCAATGGCCTAACTATGGAGTACAGCCTCAAACTTGGCCAGCAACGACTCAAGCACAAGGACAACAATGGCCTGCTGGCTACACCCAGCAGCAGGTAAATTGGTTTTTGCCCTCAACTAGTTTTCTTGTGTGTTGCTTCCCTAGTTCAAATCCCAATGTTGCATGGTGTTTGTCTATGACGTGGGCGAAATTGTTTTATAAACTAGTGAGTCTTTCTTGCCGGGTTGTGCAATATAAACTTTTTTGTTTTTGGAAATAGTGGTGTACACAGGTGTGTCATAACCTTAATCCATGAAGCATTATAATTTGAAAACATCATATTAGCCTTGTTGAAATGGAATTTGCGCCATATCTCATTGTAATGAGTTGATTACCCTTTTAGTTTATATTTGCCTTGAACCCATAAAGACATCTTCATGCATTTTTATCTACAGAAAATCATGTCATAAATGCTCTCCATTCATGCTATGCCCGACAGTTTCATGTTTTCTAGAGTTAAACATTTGTCGCACTTGTAGTGTATGTTTGACACTGTGTTTGTGCATTCTAGAATGGTGAGGTATTCCTGTTATGAAATTATCCATTCTGATTGAATACAGGCTGCCGCTTCCTATGGGGCTTATGCTGGATATGGAGGCAACTATGCTAACCCACAATTGCCTGCATCAGTTCCACAAAGCACTGCTTATGGGGCCTATCCTCCTGCATATCCTGCACAGGTGAAAAGCTGTTTCAGCCCTTATGATTTTTGCAGATTATTGTTTGCCTGATATGTTTCAGCTCTATAGTTCTTTCTTGCGAGTGTTAATTACAATTTTGCTCTGTAGAACAAGAGAAAGCAAAAAAAGTAAGGGGAATCATGTGATGGTGTTTGTGTGTGTATAGCAAGCAAAAAGAATGTAAAATATTATCGAACATACCATTGTTTTATAAGGCCTTGGCTTTTTTTTTTTTGAGGGAACACGTGCCCAGCGTATCAGGATTTTCATTGAGTTGAGTTTCACCAGTTTAATAATTGCACTAATAGTACATAAGTTTGGTTTCATTTCTCAGTTGTATTTGACTTCTCTGACCTTTTGTTACTCTTTCCATTTCTATATTTCTGCTAAACCTGAGTACTACGAATTAGATTAGTGTTGATTATTTTTTCTGAATTTCAGCCGGCACTGCCTCAACAAAATTATGCACAGCCTGTTGCAGCCCCGACACAGCAACCTGCAGCCGTTCCTCAGGCATATTATGGGAATTACTACTGA
Protein sequence

>MS.gene052391.t1

MGDSETMVAEASAAETYPSDAYNNTASNPVPEVGANVPAPAEAAGEAAAALPNSGYAFAYSGDGNAYTAGDPNSVLQAQFSATNESKQAAGVPNEDSSLVGKEALDSAMVSADHSSVNGSVDTVGLENGNASENVDGSADEKQLTDAYAALSAEEDRLWNIVTANSSDFTAWTSLIDETEKVAENNILKMRRVYDAFLAEFPLCYGYWKKYADHEARLGSADKVVEVYERAVQGVTYSVDMWLHYCIFAISTYGDPDTVRRLFERGLAYVGTDYLSFPLWDKYIEYEYMQQDWARLAMIYTRILENPNQQLDRYFNSFKELASNRPLSELRTADEAAAVAGVVSEGTDQGVEGEVHPDGADNSPKPASAVLTEAEELEKYIAIREEMYKKAKEFDSKIIGFETTIRRPYFHVRPLNIGELENWHNYLDFIEREGDLSKIVKLYERCVIACANYPEYWIRYVLCMEARESMDLANNVLARASQVFVKRQPEIHLFCARFKEQAGDIVGARAAYQLVHTEISPGLLEAIIRHANMEHRLGKLEDAFSLYEQAIAIEKGKEHSQTLPMLFAQYSRFVYLASGNSEKAREILVGGLENASLSKPLLEALLHFEAIQPQPKRVDIDFLESLVVKFITPNPENPGVASATEREELSNIFLEFLNLFGDVQSIKRVEDRHAKLFLPNRGLSELKKRHAEDFLASDKTKVSRAYSAQSPAQSVVAAYPNGPNQWPNYGVQPQTWPATTQAQGQQWPAGYTQQQAAASYGAYAGYGGNYANPQLPASVPQSTAYGAYPPAYPAQPALPQQNYAQPVAAPTQQPAAVPQAYYGNYY