AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene76222


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene76222.t1 MTR_1g112200 97.143 210 6 0 1 210 1 210 1.95e-149 414
MS.gene76222.t1 MTR_1g060120 56.931 202 76 1 17 207 20 221 9.96e-82 243
MS.gene76222.t1 MTR_1g085500 42.512 207 112 3 7 206 7 213 3.88e-52 167
MS.gene76222.t1 MTR_7g111240 45.550 191 98 4 22 207 19 208 1.41e-51 166
MS.gene76222.t1 MTR_7g111240 44.845 194 98 4 22 207 19 211 4.89e-51 164
MS.gene76222.t1 MTR_1g085140 43.367 196 104 3 19 207 20 215 3.83e-50 162
MS.gene76222.t1 MTR_6g005330 43.721 215 105 6 7 207 3 215 9.10e-48 156
MS.gene76222.t1 MTR_6g005310 43.981 216 106 6 5 207 2 215 1.86e-47 155
MS.gene76222.t1 MTR_6g005360 43.721 215 108 5 5 207 2 215 3.64e-47 154
MS.gene76222.t1 MTR_6g005340 43.981 216 106 6 5 207 2 215 6.06e-47 154
MS.gene76222.t1 MTR_1g079490 44.059 202 98 6 19 207 18 217 6.49e-47 154
MS.gene76222.t1 MTR_5g046410 42.105 209 89 7 19 206 18 215 3.70e-44 147
MS.gene76222.t1 MTR_5g046430 42.105 209 89 7 19 206 18 215 3.70e-44 147
MS.gene76222.t1 MTR_4g017030 42.105 209 89 7 19 206 18 215 3.70e-44 147
MS.gene76222.t1 MTR_4g017050 42.029 207 92 7 19 206 18 215 4.12e-44 147
MS.gene76222.t1 MTR_4g017040 40.625 224 96 8 9 206 3 215 5.34e-44 147
MS.gene76222.t1 MTR_2g019250 42.105 209 89 7 19 206 18 215 9.90e-44 146
MS.gene76222.t1 MTR_5g036570 41.753 194 105 4 22 207 26 219 9.48e-43 143
MS.gene76222.t1 MTR_1g023770 39.109 202 116 4 11 207 10 209 1.71e-42 142
MS.gene76222.t1 MTR_2g030855 40.513 195 108 5 20 207 19 212 8.94e-42 140
MS.gene76222.t1 MTR_2g031270 40.957 188 103 5 27 207 27 213 1.72e-41 140
MS.gene76222.t1 MTR_6g005350 40.686 204 99 6 19 207 18 214 1.98e-38 132
MS.gene76222.t1 MTR_2g041570 36.364 209 124 3 9 210 3 209 1.59e-37 129
MS.gene76222.t1 MTR_2g041580 36.946 203 119 3 9 204 3 203 2.45e-37 129
MS.gene76222.t1 MTR_2g030895 41.573 178 86 5 42 207 7 178 6.59e-37 127
MS.gene76222.t1 MTR_2g041550 36.816 201 122 2 9 204 3 203 1.29e-36 127
MS.gene76222.t1 MTR_8g020590 36.058 208 115 5 10 205 1 202 1.72e-35 124
MS.gene76222.t1 MTR_2g030865 41.279 172 95 4 42 207 7 178 2.45e-35 123
MS.gene76222.t1 MTR_3g019710 34.286 210 127 5 8 210 3 208 1.03e-34 122
MS.gene76222.t1 MTR_2g086640 34.597 211 112 5 12 209 8 205 2.39e-34 121
MS.gene76222.t1 MTR_8g020630 35.437 206 124 6 8 207 3 205 5.40e-34 120
MS.gene76222.t1 MTR_2g036120 33.493 209 130 4 8 210 3 208 2.76e-33 119
MS.gene76222.t1 MTR_8g020610 32.381 210 131 5 8 210 1 206 4.07e-33 118
MS.gene76222.t1 MTR_6g005380 39.109 202 108 5 7 197 9 206 4.79e-33 118
MS.gene76222.t1 MTR_3g019680 35.238 210 121 6 8 210 6 207 5.89e-33 118
MS.gene76222.t1 MTR_2g045100 36.464 181 108 4 33 210 32 208 7.49e-33 117
MS.gene76222.t1 MTR_8g020650 37.433 187 108 6 27 207 22 205 2.10e-32 116
MS.gene76222.t1 MTR_3g019650 35.714 210 120 7 8 210 6 207 2.88e-32 116
MS.gene76222.t1 MTR_8g007300 35.294 187 113 2 22 200 25 211 8.90e-32 115
MS.gene76222.t1 MTR_2g044040 32.857 210 130 5 8 210 3 208 1.16e-31 114
MS.gene76222.t1 MTR_4g010340 33.163 196 122 4 16 207 11 201 3.24e-31 113
MS.gene76222.t1 MTR_3g096330 41.176 170 88 2 15 172 563 732 4.40e-31 119
MS.gene76222.t1 MTR_8g020670 33.990 203 125 5 11 207 6 205 4.74e-31 113
MS.gene76222.t1 MTR_4g052770 33.333 213 122 5 5 209 7 207 6.41e-31 112
MS.gene76222.t1 MTR_8g020763 34.706 170 107 3 40 207 19 186 1.48e-30 111
MS.gene76222.t1 MTR_2g086620 32.057 209 120 5 12 209 9 206 1.62e-30 111
MS.gene76222.t1 MTR_2g086630 32.057 209 120 5 12 209 9 206 1.66e-30 111
MS.gene76222.t1 MTR_8g020750 33.175 211 130 4 8 210 4 211 3.36e-30 111
MS.gene76222.t1 MTR_0284s0010 32.857 210 130 5 8 210 3 208 4.48e-30 110
MS.gene76222.t1 MTR_4g052780 37.888 161 96 2 53 209 47 207 5.69e-30 110
MS.gene76222.t1 MTR_3g073170 34.574 188 112 5 29 210 27 209 2.41e-28 106
MS.gene76222.t1 MTR_2g031300 40.625 160 70 4 42 196 7 146 1.15e-27 102
MS.gene76222.t1 MTR_7g011340 31.383 188 124 2 27 210 22 208 1.40e-27 104
MS.gene76222.t1 MTR_5g031460 30.144 209 137 4 8 210 3 208 3.38e-27 103
MS.gene76222.t1 MTR_8g020770 30.144 209 139 3 8 210 4 211 4.49e-27 102
MS.gene76222.t1 MTR_8g020760 32.394 213 128 8 8 210 4 210 1.73e-26 101
MS.gene76222.t1 MTR_2g019780 32.258 186 113 7 29 207 29 208 1.21e-24 96.7
MS.gene76222.t1 MTR_3g019810 28.846 208 133 5 3 204 104 302 1.75e-21 90.1
MS.gene76222.t1 MTR_4g027415 35.200 125 70 5 27 144 22 142 9.78e-14 66.2
MS.gene76222.t1 MTR_2g072560 32.759 116 68 4 8 116 3 115 3.99e-11 59.3
MS.gene76222.t1 MTR_3g019610 34.906 106 59 4 8 106 3 105 5.05e-11 58.2
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene76222.t1 AT3G10080 60.784 204 68 3 13 207 19 219 2.02e-86 255
MS.gene76222.t1 AT3G10080 60.784 204 68 3 13 207 90 290 5.31e-86 256
MS.gene76222.t1 AT1G02335 41.935 217 118 4 1 209 1 217 3.96e-56 177
MS.gene76222.t1 AT3G04200 46.040 202 96 5 8 198 14 213 5.00e-53 170
MS.gene76222.t1 AT1G18980 45.192 208 105 4 7 207 13 218 6.68e-53 169
MS.gene76222.t1 AT1G18970 42.308 208 111 4 7 207 13 218 1.34e-51 166
MS.gene76222.t1 AT5G39160 41.429 210 113 5 7 209 11 217 2.45e-51 165
MS.gene76222.t1 AT5G39160 40.930 215 111 6 7 209 11 221 3.07e-51 165
MS.gene76222.t1 AT5G39130 40.465 215 112 6 7 209 11 221 4.44e-51 165
MS.gene76222.t1 AT5G39190 40.930 215 111 6 7 209 11 221 5.00e-51 164
MS.gene76222.t1 AT3G05950 43.981 216 106 6 7 210 12 224 7.88e-51 164
MS.gene76222.t1 AT5G26700 40.777 206 115 4 7 207 6 209 2.37e-50 162
MS.gene76222.t1 AT5G38960 44.390 205 94 6 19 207 22 222 2.62e-50 163
MS.gene76222.t1 AT3G62020 43.137 204 108 4 12 207 12 215 4.55e-50 162
MS.gene76222.t1 AT1G09560 44.041 193 101 3 22 207 23 215 3.40e-49 160
MS.gene76222.t1 AT5G39110 41.014 217 113 6 5 207 5 220 2.17e-48 158
MS.gene76222.t1 AT5G39180 40.183 219 105 6 8 207 8 219 5.71e-48 157
MS.gene76222.t1 AT3G05930 38.605 215 124 3 1 207 1 215 8.59e-48 156
MS.gene76222.t1 AT5G38910 42.326 215 109 6 6 207 7 219 8.97e-48 156
MS.gene76222.t1 AT5G38940 42.424 198 101 5 22 207 25 221 1.07e-47 156
MS.gene76222.t1 AT5G39150 40.183 219 105 6 8 207 8 219 1.40e-47 155
MS.gene76222.t1 AT5G38940 42.424 198 101 5 22 207 49 245 1.50e-47 156
MS.gene76222.t1 AT5G39120 40.000 225 106 7 5 207 2 219 3.38e-47 155
MS.gene76222.t1 AT3G62020 45.652 184 94 4 28 207 5 186 2.46e-46 152
MS.gene76222.t1 AT5G39160 39.234 209 101 6 7 209 11 199 8.69e-46 150
MS.gene76222.t1 AT5G39190 39.234 209 101 6 7 209 11 199 1.40e-45 150
MS.gene76222.t1 AT3G04180 39.500 200 110 4 20 208 23 222 5.94e-45 149
MS.gene76222.t1 AT3G04150 39.906 213 115 5 8 209 13 223 1.14e-44 149
MS.gene76222.t1 AT4G14630 39.500 200 107 5 21 207 24 222 1.89e-44 148
MS.gene76222.t1 AT3G04170 41.232 211 110 5 8 208 13 219 1.23e-43 146
MS.gene76222.t1 AT5G38930 40.201 199 106 5 21 207 24 221 1.58e-43 145
MS.gene76222.t1 AT3G04190 40.299 201 107 5 20 208 23 222 1.66e-43 145
MS.gene76222.t1 AT3G04150 38.496 226 113 6 8 209 13 236 4.07e-43 145
MS.gene76222.t1 AT5G20630 38.798 183 107 2 27 204 22 204 2.29e-39 134
MS.gene76222.t1 AT1G10460 36.181 199 113 5 22 207 18 215 4.03e-39 134
MS.gene76222.t1 AT5G61750 39.344 183 92 4 9 178 5 181 1.51e-34 122
MS.gene76222.t1 AT1G74820 31.250 224 135 6 2 209 7 227 3.28e-33 119
MS.gene76222.t1 AT1G72610 37.297 185 107 3 27 204 19 201 5.97e-33 118
MS.gene76222.t1 AT5G38910 35.681 213 89 6 6 207 7 182 5.30e-31 112
MS.gene76222.t1 AT5G38950 49.206 63 30 1 138 198 2 64 2.64e-12 61.2

Find 21 sgRNAs with CRISPR-Local

Find 42 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TCAGATTAAAGATGAGCTTT+TGG 0.263245 1.1:+79281528 MS.gene76222:CDS
GAATTTGTTATCTTCTGATT+TGG 0.267937 1.1:-79281080 None:intergenic
CCAGTTTCTTTGAAGTTTCC+TGG 0.322734 1.1:-79281163 None:intergenic
TTCTGATCCGGATCCTGTTA+TGG 0.323739 1.1:+79281045 MS.gene76222:CDS
CCAGGAAACTTCAAAGAAAC+TGG 0.339479 1.1:+79281163 MS.gene76222:CDS
GTGAATTCAAATGTGTTTCC+AGG 0.358885 1.1:+79281202 MS.gene76222:CDS
TTTGGAGAAAGGTGAGGTTA+TGG 0.393881 1.1:+79281384 MS.gene76222:CDS
TGAAAATGCACAAGTCCTCT+TGG 0.414763 1.1:-79281412 None:intergenic
AACAGAAGTTGCTATTGTAT+TGG 0.415183 1.1:+79281306 MS.gene76222:CDS
GCTATGCAGTAATCCATAAC+AGG 0.434646 1.1:-79281058 None:intergenic
ATAGCAACTTCTGTTGCTCT+TGG 0.436530 1.1:-79281298 None:intergenic
CAGTAATCCATAACAGGATC+CGG 0.469062 1.1:-79281052 None:intergenic
TTTCCAGGTTTAAACACTCT+TGG 0.469660 1.1:+79281217 MS.gene76222:CDS
CAGCAATGATGAAACACATA+AGG 0.504374 1.1:-79281015 None:intergenic
ACACATAAGGACTAAAAGAA+TGG 0.521199 1.1:-79281002 None:intergenic
AGAAGTTGCTATTGTATTGG+AGG 0.525508 1.1:+79281309 MS.gene76222:CDS
GAAGTTGCTATTGTATTGGA+GGG 0.550409 1.1:+79281310 MS.gene76222:CDS
ACTCCAAGAGTGTTTAAACC+TGG 0.567991 1.1:-79281220 None:intergenic
GAAATCTTCAACTGTTGCTG+TGG 0.571728 1.1:-79281119 None:intergenic
AGCTTTCTAAGTTAAAGAAG+AGG 0.576087 1.1:+79281578 MS.gene76222:CDS
GTTGCTCTTGGATGAAAGTG+AGG 0.622903 1.1:-79281286 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! AAAATTCCAAATGTTGTTTT+TGG + chr1.1:79281502-79281521 MS.gene76222:CDS 20.0%
!! CAAGATTTTTGCTAAAGTTT+TGG + chr1.1:79281366-79281385 MS.gene76222:CDS 25.0%
!!! GAATTTGTTATCTTCTGATT+TGG - chr1.1:79281083-79281102 None:intergenic 25.0%
AAACTACCAAAAATAGTAGC+AGG - chr1.1:79281457-79281476 None:intergenic 30.0%
ACACATAAGGACTAAAAGAA+TGG - chr1.1:79281005-79281024 None:intergenic 30.0%
AGCTTTCTAAGTTAAAGAAG+AGG + chr1.1:79281578-79281597 MS.gene76222:CDS 30.0%
TGAGATCCAAAAACAACATT+TGG - chr1.1:79281511-79281530 None:intergenic 30.0%
TTGGAGGGAAAAATTTATTC+AGG + chr1.1:79281325-79281344 MS.gene76222:CDS 30.0%
! AACAGAAGTTGCTATTGTAT+TGG + chr1.1:79281306-79281325 MS.gene76222:CDS 30.0%
! AGAAACTGGATTTTCTTCAA+TGG + chr1.1:79281177-79281196 MS.gene76222:CDS 30.0%
! AGCTTTTGGACTTAATTCTA+AGG + chr1.1:79281555-79281574 MS.gene76222:CDS 30.0%
! GAAACTGGATTTTCTTCAAT+GGG + chr1.1:79281178-79281197 MS.gene76222:CDS 30.0%
! TCAGATTAAAGATGAGCTTT+TGG + chr1.1:79281528-79281547 MS.gene76222:CDS 30.0%
!! AGTTTTGATAGTCAAAATCC+TGG + chr1.1:79281472-79281491 MS.gene76222:CDS 30.0%
!! TTTGGAATTTTCATCAAACC+AGG - chr1.1:79281493-79281512 None:intergenic 30.0%
!! TTTTCTGGTATAAAACTACC+AGG + chr1.1:79281145-79281164 MS.gene76222:CDS 30.0%
!!! GTTGAAGATTTCACTTTTTC+TGG + chr1.1:79281130-79281149 MS.gene76222:CDS 30.0%
!!! TTTGCTAAAGTTTTGGAGAA+AGG + chr1.1:79281373-79281392 MS.gene76222:CDS 30.0%
CAGCAATGATGAAACACATA+AGG - chr1.1:79281018-79281037 None:intergenic 35.0%
GTGAATTCAAATGTGTTTCC+AGG + chr1.1:79281202-79281221 MS.gene76222:CDS 35.0%
TTTCCAGGTTTAAACACTCT+TGG + chr1.1:79281217-79281236 MS.gene76222:CDS 35.0%
! AGAAGTTGCTATTGTATTGG+AGG + chr1.1:79281309-79281328 MS.gene76222:CDS 35.0%
! GAAGTTGCTATTGTATTGGA+GGG + chr1.1:79281310-79281329 MS.gene76222:CDS 35.0%
! GATGTTCCTGCTACTATTTT+TGG + chr1.1:79281448-79281467 MS.gene76222:CDS 35.0%
! GTGCATTTTCAGATGAATGT+TGG + chr1.1:79281424-79281443 MS.gene76222:CDS 35.0%
!! GAGCTTTTGGAAAAAGCTTT+TGG + chr1.1:79281541-79281560 MS.gene76222:CDS 35.0%
!! GTTAGAGCAGATTTTGATGT+TGG + chr1.1:79281250-79281269 MS.gene76222:CDS 35.0%
!!! TAAAGTTTTGGAGAAAGGTG+AGG + chr1.1:79281378-79281397 MS.gene76222:CDS 35.0%
ATAGCAACTTCTGTTGCTCT+TGG - chr1.1:79281301-79281320 None:intergenic 40.0%
CAGTAATCCATAACAGGATC+CGG - chr1.1:79281055-79281074 None:intergenic 40.0%
CCAGGAAACTTCAAAGAAAC+TGG + chr1.1:79281163-79281182 MS.gene76222:CDS 40.0%
GAAATCTTCAACTGTTGCTG+TGG - chr1.1:79281122-79281141 None:intergenic 40.0%
GCTATGCAGTAATCCATAAC+AGG - chr1.1:79281061-79281080 None:intergenic 40.0%
TGAAAATGCACAAGTCCTCT+TGG - chr1.1:79281415-79281434 None:intergenic 40.0%
TTTGGAGAAAGGTGAGGTTA+TGG + chr1.1:79281384-79281403 MS.gene76222:CDS 40.0%
! GAGGTTATGGTTTTTCCAAG+AGG + chr1.1:79281397-79281416 MS.gene76222:CDS 40.0%
!! ACTCCAAGAGTGTTTAAACC+TGG - chr1.1:79281223-79281242 None:intergenic 40.0%
!! AGAGCAGATTTTGATGTTGG+TGG + chr1.1:79281253-79281272 MS.gene76222:CDS 40.0%
!! CCAGTTTCTTTGAAGTTTCC+TGG - chr1.1:79281166-79281185 None:intergenic 40.0%
!!! TGCTGCTTTTTGTTCTGATC+CGG + chr1.1:79281033-79281052 MS.gene76222:CDS 40.0%
GTTGCTCTTGGATGAAAGTG+AGG - chr1.1:79281289-79281308 None:intergenic 45.0%
TTCTGATCCGGATCCTGTTA+TGG + chr1.1:79281045-79281064 MS.gene76222:CDS 45.0%


Chromosome Type Strat End Strand Name
chr1.1 gene 79280983 79281615 79280983 ID=MS.gene76222
chr1.1 mRNA 79280983 79281615 79280983 ID=MS.gene76222.t1;Parent=MS.gene76222
chr1.1 exon 79280983 79281615 79280983 ID=MS.gene76222.t1.exon1;Parent=MS.gene76222.t1
chr1.1 CDS 79280983 79281615 79280983 ID=cds.MS.gene76222.t1;Parent=MS.gene76222.t1
Gene Sequence

>MS.gene76222

ATGATCAACAACAAAGTAACCATTCTTTTAGTCCTTATGTGTTTCATCATTGCTGCTTTTTGTTCTGATCCGGATCCTGTTATGGATTACTGCATAGCCAAATCAGAAGATAACAAATTCAGTTGCAAAAACTCATCCACAGCAACAGTTGAAGATTTCACTTTTTCTGGTATAAAACTACCAGGAAACTTCAAAGAAACTGGATTTTCTTCAATGGGAGTGAATTCAAATGTGTTTCCAGGTTTAAACACTCTTGGAGTTTCATTTGTTAGAGCAGATTTTGATGTTGGTGGTGTTAATGTACCTCACTTTCATCCAAGAGCAACAGAAGTTGCTATTGTATTGGAGGGAAAAATTTATTCAGGTTTTGTTGATACTAAGAACAAGATTTTTGCTAAAGTTTTGGAGAAAGGTGAGGTTATGGTTTTTCCAAGAGGACTTGTGCATTTTCAGATGAATGTTGGTGATGTTCCTGCTACTATTTTTGGTAGTTTTGATAGTCAAAATCCTGGTTTGATGAAAATTCCAAATGTTGTTTTTGGATCTCAGATTAAAGATGAGCTTTTGGAAAAAGCTTTTGGACTTAATTCTAAGGAGCTTTCTAAGTTAAAGAAGAGGTTTACTTCTAGCTAA

Protein sequence

>MS.gene76222.t1

MINNKVTILLVLMCFIIAAFCSDPDPVMDYCIAKSEDNKFSCKNSSTATVEDFTFSGIKLPGNFKETGFSSMGVNSNVFPGLNTLGVSFVRADFDVGGVNVPHFHPRATEVAIVLEGKIYSGFVDTKNKIFAKVLEKGEVMVFPRGLVHFQMNVGDVPATIFGSFDSQNPGLMKIPNVVFGSQIKDELLEKAFGLNSKELSKLKKRFTSS