Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene76319.t1 | XP_013468026.1 | 98.9 | 93 | 1 | 0 | 1 | 93 | 109 | 201 | 5.50E-50 | 206.5 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene76319.t1 | A0A072VKV4 | 98.9 | 93 | 1 | 0 | 1 | 93 | 109 | 201 | 4.0e-50 | 206.5 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene050156 | MS.gene76319 | 0.803546 | 3.31E-49 | -1.69E-46 |
| MS.gene055818 | MS.gene76319 | 0.803983 | 2.69E-49 | -1.69E-46 |
| MS.gene058010 | MS.gene76319 | 0.820402 | 6.99E-53 | -1.69E-46 |
| MS.gene058515 | MS.gene76319 | 0.802071 | 6.68E-49 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 26 sgRNAs with CRISPR-Local
Find 38 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| ATTAGGTTTACTTACGGAAT+TGG | 0.296469 | 3.4:-29881031 | MS.gene76319:CDS |
| AATATCCATCTAAAAGCATT+AGG | 0.316447 | 3.4:-29881048 | MS.gene76319:CDS |
| ATACTTGGATTCTTGAGGTT+TGG | 0.380840 | 3.4:+29880745 | None:intergenic |
| TTAGGTTTACTTACGGAATT+GGG | 0.426191 | 3.4:-29881030 | MS.gene76319:CDS |
| GTTCTGTATGGGCATTAGAT+TGG | 0.440759 | 3.4:-29880530 | MS.gene76319:CDS |
| TCACATTTAATTGAACAATC+AGG | 0.451414 | 3.4:+29880486 | None:intergenic |
| TGCAAAAGGAGGAGCAACTT+GGG | 0.454385 | 3.4:-29880772 | MS.gene76319:CDS |
| TCAGGCTCCCCATGCATCCT+AGG | 0.455090 | 3.4:+29880504 | None:intergenic |
| TTCATCTGCCACTGTTCCTA+AGG | 0.455578 | 3.4:-29880951 | MS.gene76319:intron |
| AAAAGCATTAGGTTTACTTA+CGG | 0.463447 | 3.4:-29881037 | MS.gene76319:CDS |
| ATGCAAAAGGAGGAGCAACT+TGG | 0.467583 | 3.4:-29880773 | MS.gene76319:CDS |
| GGGCATTAGATTGGTGTCCT+AGG | 0.483270 | 3.4:-29880521 | MS.gene76319:CDS |
| TGAATGTTGGAGGTTCTGTA+TGG | 0.503920 | 3.4:-29880542 | MS.gene76319:CDS |
| ATTGGTGTCCTAGGATGCAT+GGG | 0.532952 | 3.4:-29880512 | MS.gene76319:CDS |
| GATTGGTGTCCTAGGATGCA+TGG | 0.541625 | 3.4:-29880513 | MS.gene76319:CDS |
| AAACCATACTTGGATTCTTG+AGG | 0.544459 | 3.4:+29880740 | None:intergenic |
| AGAGACTTTGTGATGAATGT+TGG | 0.551861 | 3.4:-29880555 | MS.gene76319:CDS |
| TTAAATGTGCAGAGAATGGA+AGG | 0.560081 | 3.4:-29881077 | None:intergenic |
| GACTTTGTGATGAATGTTGG+AGG | 0.568156 | 3.4:-29880552 | MS.gene76319:CDS |
| TCAGCATAATATGCAAAAGG+AGG | 0.605517 | 3.4:-29880783 | MS.gene76319:intron |
| TGATTGTTCAATTAAATGTG+AGG | 0.630368 | 3.4:-29880484 | None:intergenic |
| GAATGTTGGAGGTTCTGTAT+GGG | 0.641333 | 3.4:-29880541 | MS.gene76319:CDS |
| GCAAAAGGAGGAGCAACTTG+GGG | 0.648508 | 3.4:-29880771 | MS.gene76319:CDS |
| GACTTGTACCTTAGGAACAG+TGG | 0.679559 | 3.4:+29880943 | None:intergenic |
| TTGGTGTCCTAGGATGCATG+GGG | 0.719461 | 3.4:-29880511 | MS.gene76319:CDS |
| ACAGTGGCAGATGAAAACTG+AGG | 0.800494 | 3.4:+29880959 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | ATTAAATTAGAGTAAAATAT+AGG | + | chr3.4:29880635-29880654 | None:intergenic | 10.0% |
| !!! | TAAGTATATATTTTGCTTTT+AGG | - | chr3.4:29880684-29880703 | MS.gene76319:intron | 15.0% |
| !! | AAATTAGAGTAAAATATAGG+AGG | + | chr3.4:29880632-29880651 | None:intergenic | 20.0% |
| !! | TTTATTCAATGACATTTCTA+TGG | - | chr3.4:29880856-29880875 | MS.gene76319:intron | 20.0% |
| ! | AGTTAATATCAAACCATACT+TGG | + | chr3.4:29880823-29880842 | None:intergenic | 25.0% |
| ! | TCACATTTAATTGAACAATC+AGG | + | chr3.4:29881067-29881086 | None:intergenic | 25.0% |
| !! | TTTTCAGCATAATATGCAAA+AGG | - | chr3.4:29880764-29880783 | MS.gene76319:CDS | 25.0% |
| !!! | AAAAGCATTAGGTTTACTTA+CGG | - | chr3.4:29880513-29880532 | MS.gene76319:CDS | 25.0% |
| !!! | AATATCCATCTAAAAGCATT+AGG | - | chr3.4:29880502-29880521 | MS.gene76319:CDS | 25.0% |
| !!! | GATATTAACTTATTTTGTCG+TGG | - | chr3.4:29880833-29880852 | MS.gene76319:intron | 25.0% |
| ATATACTATAACTTCATGCC+TGG | + | chr3.4:29880664-29880683 | None:intergenic | 30.0% | |
| ATTAGGTTTACTTACGGAAT+TGG | - | chr3.4:29880519-29880538 | MS.gene76319:CDS | 30.0% | |
| CTCTAATTTAATTGTTTGCC+AGG | - | chr3.4:29880643-29880662 | MS.gene76319:intron | 30.0% | |
| TTAGGTTTACTTACGGAATT+GGG | - | chr3.4:29880520-29880539 | MS.gene76319:CDS | 30.0% | |
| ! | GTAAACCTAATGCTTTTAGA+TGG | + | chr3.4:29880510-29880529 | None:intergenic | 30.0% |
| ! | TCGCTGATTATTGTTTAACT+TGG | - | chr3.4:29880953-29880972 | MS.gene76319:CDS | 30.0% |
| AAACCATACTTGGATTCTTG+AGG | + | chr3.4:29880813-29880832 | None:intergenic | 35.0% | |
| AAACCTCAAGAATCCAAGTA+TGG | - | chr3.4:29880807-29880826 | MS.gene76319:intron | 35.0% | |
| AACTTGGCTAAACACTTTGT+TGG | - | chr3.4:29880969-29880988 | MS.gene76319:CDS | 35.0% | |
| AGAGACTTTGTGATGAATGT+TGG | - | chr3.4:29880995-29881014 | MS.gene76319:CDS | 35.0% | |
| ATACTTGGATTCTTGAGGTT+TGG | + | chr3.4:29880808-29880827 | None:intergenic | 35.0% | |
| TCAGCATAATATGCAAAAGG+AGG | - | chr3.4:29880767-29880786 | MS.gene76319:CDS | 35.0% | |
| GAATGTTGGAGGTTCTGTAT+GGG | - | chr3.4:29881009-29881028 | MS.gene76319:CDS | 40.0% | |
| GACTTTGTGATGAATGTTGG+AGG | - | chr3.4:29880998-29881017 | MS.gene76319:CDS | 40.0% | |
| GTTCTGTATGGGCATTAGAT+TGG | - | chr3.4:29881020-29881039 | MS.gene76319:CDS | 40.0% | |
| TATAGGAGGACTTGTACCTT+AGG | + | chr3.4:29880618-29880637 | None:intergenic | 40.0% | |
| TGAATGTTGGAGGTTCTGTA+TGG | - | chr3.4:29881008-29881027 | MS.gene76319:CDS | 40.0% | |
| ACAGTGGCAGATGAAAACTG+AGG | + | chr3.4:29880594-29880613 | None:intergenic | 45.0% | |
| ATGCAAAAGGAGGAGCAACT+TGG | - | chr3.4:29880777-29880796 | MS.gene76319:CDS | 45.0% | |
| GACTTGTACCTTAGGAACAG+TGG | + | chr3.4:29880610-29880629 | None:intergenic | 45.0% | |
| TGCAAAAGGAGGAGCAACTT+GGG | - | chr3.4:29880778-29880797 | MS.gene76319:CDS | 45.0% | |
| TTCATCTGCCACTGTTCCTA+AGG | - | chr3.4:29880599-29880618 | MS.gene76319:intron | 45.0% | |
| ! | ATTGGTGTCCTAGGATGCAT+GGG | - | chr3.4:29881038-29881057 | MS.gene76319:CDS | 45.0% |
| GCAAAAGGAGGAGCAACTTG+GGG | - | chr3.4:29880779-29880798 | MS.gene76319:CDS | 50.0% | |
| ! | GATTGGTGTCCTAGGATGCA+TGG | - | chr3.4:29881037-29881056 | MS.gene76319:CDS | 50.0% |
| ! | GGGCATTAGATTGGTGTCCT+AGG | - | chr3.4:29881029-29881048 | MS.gene76319:CDS | 50.0% |
| ! | TTGGTGTCCTAGGATGCATG+GGG | - | chr3.4:29881039-29881058 | MS.gene76319:CDS | 50.0% |
| TCAGGCTCCCCATGCATCCT+AGG | + | chr3.4:29881049-29881068 | None:intergenic | 60.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr3.4 | gene | 29880485 | 29881087 | 29880485 | ID=MS.gene76319 |
| chr3.4 | mRNA | 29880485 | 29881087 | 29880485 | ID=MS.gene76319.t1;Parent=MS.gene76319 |
| chr3.4 | exon | 29880952 | 29881087 | 29880952 | ID=MS.gene76319.t1.exon1;Parent=MS.gene76319.t1 |
| chr3.4 | CDS | 29880952 | 29881087 | 29880952 | ID=cds.MS.gene76319.t1;Parent=MS.gene76319.t1 |
| chr3.4 | exon | 29880749 | 29880801 | 29880749 | ID=MS.gene76319.t1.exon2;Parent=MS.gene76319.t1 |
| chr3.4 | CDS | 29880749 | 29880801 | 29880749 | ID=cds.MS.gene76319.t1;Parent=MS.gene76319.t1 |
| chr3.4 | exon | 29880485 | 29880575 | 29880485 | ID=MS.gene76319.t1.exon3;Parent=MS.gene76319.t1 |
| chr3.4 | CDS | 29880485 | 29880575 | 29880485 | ID=cds.MS.gene76319.t1;Parent=MS.gene76319.t1 |
| Gene Sequence |
| Protein sequence |
