Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene76702.t1 | XP_003605289.1 | 97.8 | 901 | 19 | 1 | 1 | 901 | 1 | 900 | 0.00E+00 | 1728 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene76702.t1 | Q6Z3U3 | 49.4 | 932 | 404 | 20 | 3 | 885 | 14 | 926 | 1.4e-215 | 751.1 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene76702.t1 | G7JIB0 | 97.8 | 901 | 19 | 1 | 1 | 901 | 1 | 900 | 0.0e+00 | 1728.0 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|---|---|
| MS.gene76702.t1 | TF | B3 |
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene052498 | MS.gene76702 | 0.804193 | 2.43E-49 | -1.69E-46 |
| MS.gene057148 | MS.gene76702 | -0.804221 | 2.40E-49 | -1.69E-46 |
| MS.gene059778 | MS.gene76702 | 0.822216 | 2.67E-53 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene76702.t1 | MTR_4g028130 | 97.780 | 901 | 19 | 1 | 1 | 901 | 1 | 900 | 0.0 | 1824 |
| MS.gene76702.t1 | MTR_4g028130 | 93.785 | 901 | 19 | 1 | 1 | 901 | 1 | 864 | 0.0 | 1734 |
| MS.gene76702.t1 | MTR_4g028130 | 97.816 | 641 | 13 | 1 | 1 | 641 | 1 | 640 | 0.0 | 1308 |
| MS.gene76702.t1 | MTR_3g467380 | 48.115 | 902 | 416 | 13 | 19 | 887 | 16 | 898 | 0.0 | 789 |
| MS.gene76702.t1 | MTR_5g075410 | 43.667 | 829 | 401 | 12 | 1 | 815 | 1 | 777 | 0.0 | 618 |
| MS.gene76702.t1 | MTR_2g011940 | 51.443 | 589 | 214 | 14 | 270 | 839 | 299 | 834 | 3.13e-179 | 541 |
| MS.gene76702.t1 | MTR_2g011940 | 43.411 | 129 | 56 | 2 | 4 | 132 | 20 | 131 | 1.36e-26 | 117 |
| MS.gene76702.t1 | MTR_2g011940 | 55.978 | 368 | 124 | 4 | 270 | 637 | 308 | 637 | 6.11e-128 | 400 |
| MS.gene76702.t1 | MTR_2g011940 | 43.411 | 129 | 56 | 2 | 4 | 132 | 29 | 140 | 1.00e-26 | 117 |
| MS.gene76702.t1 | MTR_2g011940 | 55.978 | 368 | 124 | 4 | 270 | 637 | 308 | 637 | 2.01e-127 | 399 |
| MS.gene76702.t1 | MTR_2g011940 | 43.411 | 129 | 56 | 2 | 4 | 132 | 29 | 140 | 1.79e-26 | 116 |
| MS.gene76702.t1 | MTR_7g073240 | 55.357 | 224 | 97 | 2 | 199 | 419 | 3 | 226 | 3.74e-70 | 237 |
| MS.gene76702.t1 | MTR_7g073240 | 53.147 | 143 | 52 | 4 | 485 | 625 | 230 | 359 | 2.59e-39 | 150 |
| MS.gene76702.t1 | MTR_7g083700 | 39.806 | 103 | 61 | 1 | 314 | 415 | 126 | 228 | 2.14e-18 | 87.8 |
| MS.gene76702.t1 | MTR_7g059330 | 35.714 | 98 | 62 | 1 | 314 | 410 | 623 | 720 | 1.20e-14 | 79.0 |
| MS.gene76702.t1 | MTR_1g058350 | 36.090 | 133 | 65 | 6 | 295 | 414 | 54 | 179 | 1.57e-14 | 76.3 |
| MS.gene76702.t1 | MTR_8g023990 | 37.273 | 110 | 61 | 4 | 314 | 417 | 171 | 278 | 3.27e-14 | 77.0 |
| MS.gene76702.t1 | MTR_7g117455 | 40.659 | 91 | 49 | 3 | 314 | 401 | 117 | 205 | 9.38e-14 | 75.1 |
| MS.gene76702.t1 | MTR_7g105370 | 39.252 | 107 | 58 | 5 | 319 | 421 | 65 | 168 | 9.95e-13 | 70.5 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene76702.t1 | AT4G32010 | 50.350 | 858 | 309 | 15 | 1 | 852 | 1 | 747 | 0.0 | 801 |
| MS.gene76702.t1 | AT4G32010 | 50.116 | 862 | 309 | 16 | 1 | 852 | 1 | 751 | 0.0 | 795 |
| MS.gene76702.t1 | AT2G30470 | 51.476 | 779 | 305 | 20 | 1 | 765 | 6 | 725 | 0.0 | 709 |
| MS.gene76702.t1 | AT4G21550 | 34.291 | 592 | 288 | 15 | 3 | 570 | 14 | 528 | 6.48e-92 | 301 |
| MS.gene76702.t1 | AT4G21550 | 34.797 | 592 | 286 | 12 | 3 | 571 | 14 | 528 | 2.03e-88 | 297 |
| MS.gene76702.t1 | AT4G21550 | 37.795 | 127 | 57 | 3 | 687 | 812 | 550 | 655 | 4.81e-12 | 70.5 |
| MS.gene76702.t1 | AT4G21550 | 33.681 | 576 | 282 | 13 | 3 | 555 | 14 | 512 | 1.42e-87 | 289 |
| MS.gene76702.t1 | AT3G26790 | 39.450 | 109 | 65 | 1 | 314 | 421 | 91 | 199 | 8.21e-20 | 92.0 |
| MS.gene76702.t1 | AT5G06250 | 32.948 | 173 | 95 | 5 | 314 | 467 | 45 | 215 | 1.13e-16 | 82.0 |
| MS.gene76702.t1 | AT5G06250 | 32.948 | 173 | 95 | 5 | 314 | 467 | 45 | 215 | 1.13e-16 | 82.0 |
| MS.gene76702.t1 | AT5G06250 | 32.948 | 173 | 95 | 5 | 314 | 467 | 45 | 215 | 1.16e-16 | 81.6 |
| MS.gene76702.t1 | AT1G13260 | 40.000 | 110 | 59 | 5 | 314 | 418 | 187 | 294 | 1.97e-15 | 79.3 |
| MS.gene76702.t1 | AT3G24650 | 37.931 | 116 | 70 | 2 | 314 | 427 | 571 | 686 | 2.24e-15 | 81.3 |
| MS.gene76702.t1 | AT1G28300 | 40.741 | 108 | 60 | 3 | 294 | 398 | 149 | 255 | 3.49e-15 | 79.0 |
| MS.gene76702.t1 | AT2G36080 | 39.815 | 108 | 56 | 4 | 314 | 414 | 37 | 142 | 4.15e-15 | 74.7 |
| MS.gene76702.t1 | AT2G36080 | 39.815 | 108 | 56 | 4 | 314 | 414 | 37 | 142 | 4.15e-15 | 74.7 |
| MS.gene76702.t1 | AT2G36080 | 39.815 | 108 | 56 | 4 | 314 | 414 | 37 | 142 | 4.75e-15 | 76.3 |
| MS.gene76702.t1 | AT2G36080 | 39.815 | 108 | 56 | 4 | 314 | 414 | 37 | 142 | 4.88e-15 | 76.3 |
| MS.gene76702.t1 | AT5G06250 | 36.923 | 130 | 56 | 5 | 314 | 419 | 45 | 172 | 5.22e-15 | 76.6 |
| MS.gene76702.t1 | AT5G06250 | 36.923 | 130 | 56 | 5 | 314 | 419 | 45 | 172 | 6.27e-15 | 76.6 |
| MS.gene76702.t1 | AT4G01500 | 38.261 | 115 | 59 | 4 | 297 | 401 | 9 | 121 | 2.69e-14 | 75.5 |
| MS.gene76702.t1 | AT1G68840 | 43.011 | 93 | 46 | 4 | 314 | 401 | 187 | 277 | 3.79e-14 | 75.5 |
| MS.gene76702.t1 | AT1G68840 | 43.011 | 93 | 46 | 4 | 314 | 401 | 187 | 277 | 3.79e-14 | 75.5 |
| MS.gene76702.t1 | AT2G46870 | 39.560 | 91 | 50 | 3 | 314 | 401 | 34 | 122 | 8.11e-14 | 73.9 |
| MS.gene76702.t1 | AT1G01030 | 40.659 | 91 | 49 | 3 | 314 | 401 | 55 | 143 | 7.48e-13 | 71.2 |
| MS.gene76702.t1 | AT3G11580 | 36.207 | 116 | 57 | 4 | 314 | 414 | 28 | 141 | 8.09e-13 | 69.3 |
| MS.gene76702.t1 | AT3G11580 | 36.207 | 116 | 57 | 4 | 314 | 414 | 28 | 141 | 8.09e-13 | 69.3 |
| MS.gene76702.t1 | AT3G11580 | 36.207 | 116 | 57 | 4 | 314 | 414 | 28 | 141 | 8.09e-13 | 69.3 |
| MS.gene76702.t1 | AT1G01030 | 40.659 | 91 | 49 | 3 | 314 | 401 | 55 | 143 | 9.25e-13 | 71.2 |
| MS.gene76702.t1 | AT3G11580 | 36.207 | 116 | 57 | 4 | 314 | 414 | 28 | 141 | 4.67e-12 | 69.3 |
| MS.gene76702.t1 | AT3G11580 | 36.207 | 116 | 57 | 4 | 314 | 414 | 28 | 141 | 5.08e-12 | 67.8 |
Find 239 sgRNAs with CRISPR-Local
Find 0 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| GGATGTTTCAATTTAGATTT+TGG | 0.133418 | 4.1:-71338918 | MS.gene76702:CDS |
| TACATCCTGCTATTATTATT+TGG | 0.167699 | 4.1:+71338895 | None:intergenic |
| CAACTCTTGATCTGTAATTC+TGG | 0.172835 | 4.1:+71339380 | None:intergenic |
| TTACCCTCTGCTGGAGAATT+TGG | 0.185934 | 4.1:-71339756 | MS.gene76702:CDS |
| GCATACCCCTCACATTTAAT+TGG | 0.234093 | 4.1:+71339877 | None:intergenic |
| AAGAAAAGAACTCGAAATAT+TGG | 0.272591 | 4.1:-71337870 | MS.gene76702:CDS |
| GGACGTGTATGGAGAACTTT+TGG | 0.278484 | 4.1:-71336973 | MS.gene76702:CDS |
| TCAAGTGAAACTTCCTATTC+TGG | 0.278901 | 4.1:-71338089 | MS.gene76702:CDS |
| GAGGCCTTGCTATACGTATC+TGG | 0.288912 | 4.1:+71339448 | None:intergenic |
| ATTACTTCAGCACATGCTTT+TGG | 0.292122 | 4.1:+71339120 | None:intergenic |
| CCAGCAGAGGGTAAGACTTC+TGG | 0.293216 | 4.1:+71339765 | None:intergenic |
| CCTATATTAAGTGGTTATTC+TGG | 0.299020 | 4.1:-71338050 | MS.gene76702:CDS |
| CAAACCTCACAATGTTGATT+TGG | 0.320944 | 4.1:-71339811 | MS.gene76702:CDS |
| ATCAGTTGCTTCCGCGATAT+TGG | 0.327434 | 4.1:-71339404 | MS.gene76702:CDS |
| ATTCAGGAGCCTCCTGTGTT+TGG | 0.329853 | 4.1:-71337255 | MS.gene76702:intron |
| CAACTGCAAGCTGGCGATAC+TGG | 0.336996 | 4.1:-71338835 | MS.gene76702:intron |
| TCTTGCCCACTATTCTCTTC+AGG | 0.340208 | 4.1:+71335056 | None:intergenic |
| TGCCCTTCCCACTTGGTGGC+TGG | 0.342375 | 4.1:+71335600 | None:intergenic |
| ACAACACTTGGCTTAACTTC+TGG | 0.343319 | 4.1:+71337756 | None:intergenic |
| CCCTGAGGGGAAACTTATAA+TGG | 0.343393 | 4.1:-71338253 | MS.gene76702:CDS |
| AGAGTTGGCCTAGGAGGTTT+AGG | 0.345670 | 4.1:+71339507 | None:intergenic |
| TTCCCTTGGATTCAATTCAT+TGG | 0.348370 | 4.1:+71336840 | None:intergenic |
| CACTGGAGCTGAAACTTACT+TGG | 0.350730 | 4.1:-71337809 | MS.gene76702:CDS |
| ACTTTCTAGAGAGCGCATTT+AGG | 0.352085 | 4.1:+71337991 | None:intergenic |
| TCAAGAGTTGCAGCAAATAT+CGG | 0.354064 | 4.1:-71339367 | MS.gene76702:CDS |
| AATGAAAAGCATAATGAATC+TGG | 0.354130 | 4.1:-71339951 | MS.gene76702:CDS |
| CGACATTTAACTCCAGTTGA+TGG | 0.354939 | 4.1:-71335410 | MS.gene76702:CDS |
| TTACTTGAATCTAATTCATT+TGG | 0.362360 | 4.1:+71335365 | None:intergenic |
| TTGTCTTCCCTCACTCTCAT+TGG | 0.362735 | 4.1:+71335127 | None:intergenic |
| ATGTGAGGGGTATGCAAGTT+GGG | 0.375012 | 4.1:-71339869 | MS.gene76702:CDS |
| CAGCATCAGCCACTTGGTCT+TGG | 0.376062 | 4.1:+71335342 | None:intergenic |
| TCCCTTGGATTCAATTCATT+GGG | 0.378007 | 4.1:+71336841 | None:intergenic |
| AGGACATTATGAAAGGGATT+TGG | 0.378577 | 4.1:+71339600 | None:intergenic |
| TTAGCAAATGCTGCGGTACT+TGG | 0.380392 | 4.1:-71335713 | MS.gene76702:CDS |
| GTCTACTTCTGATTCATCCT+TGG | 0.381182 | 4.1:+71335442 | None:intergenic |
| AGGCCCTCAGGTTGAGAGAT+AGG | 0.381204 | 4.1:+71338976 | None:intergenic |
| ATTACCTGTGCAAGTACTTC+AGG | 0.390989 | 4.1:-71340466 | MS.gene76702:CDS |
| ATATTTGTGGTACGATCTAC+TGG | 0.393389 | 4.1:-71337222 | MS.gene76702:CDS |
| TGGCCTTCTTCAGTCTCAAA+AGG | 0.396098 | 4.1:-71338030 | MS.gene76702:CDS |
| ACTTACTTGGGAAGAGGCTC+AGG | 0.399619 | 4.1:-71337796 | MS.gene76702:CDS |
| GTCATCATGCTCAGAGTGTT+TGG | 0.401168 | 4.1:+71335263 | None:intergenic |
| GGAAACTTATAATGGGGTTC+AGG | 0.403096 | 4.1:-71338245 | MS.gene76702:CDS |
| CAATTTCATCGTGATTCAAA+TGG | 0.411668 | 4.1:-71339717 | MS.gene76702:CDS |
| TTCCTTCCTCGTCCTTCGGC+AGG | 0.411704 | 4.1:+71339426 | None:intergenic |
| CCAGAATAACCACTTAATAT+AGG | 0.414414 | 4.1:+71338050 | None:intergenic |
| ACTCTATCTCCAGGCTTAGA+AGG | 0.416607 | 4.1:-71339489 | MS.gene76702:CDS |
| TGAATTGAATCCAAGGGAAC+TGG | 0.417947 | 4.1:-71336836 | MS.gene76702:CDS |
| AGAAAAGAACTCGAAATATT+GGG | 0.421447 | 4.1:-71337869 | MS.gene76702:CDS |
| TGCTTCTGCAGTTCATGAAC+AGG | 0.426089 | 4.1:-71335087 | MS.gene76702:CDS |
| CTTGAATTGCCAACCGGATC+GGG | 0.426831 | 4.1:-71335303 | MS.gene76702:CDS |
| TCTCCATACACGTCCACTTA+GGG | 0.429073 | 4.1:+71336981 | None:intergenic |
| TCTGGTTTGATTTCTCTAGA+AGG | 0.431001 | 4.1:+71339783 | None:intergenic |
| GCTCTGGATCGTGAATCTTC+TGG | 0.431530 | 4.1:-71335746 | MS.gene76702:CDS |
| AACTCTTGATCTGTAATTCT+GGG | 0.432750 | 4.1:+71339381 | None:intergenic |
| TCATTCTCTGAACCATCAAC+TGG | 0.433510 | 4.1:+71335398 | None:intergenic |
| AATGTCACCTCCACTTCTAT+TGG | 0.433907 | 4.1:-71339553 | MS.gene76702:CDS |
| GCTTGCATGTCTTCCCGATC+CGG | 0.434187 | 4.1:+71335290 | None:intergenic |
| ATGTTCCATGCAAAAGAATC+TGG | 0.435693 | 4.1:-71340677 | MS.gene76702:CDS |
| CCTGTGTTTCCTTCTAAGCC+TGG | 0.440594 | 4.1:+71339480 | None:intergenic |
| CACGATCCAGAGCTTGCCTC+TGG | 0.441243 | 4.1:+71335756 | None:intergenic |
| CCTTGGCAGCATCAGCCACT+TGG | 0.441564 | 4.1:+71335336 | None:intergenic |
| ATTCTCATAAACACCAGAAT+AGG | 0.444115 | 4.1:+71338076 | None:intergenic |
| TTCACTTGAATGAGAACCAT+TGG | 0.445941 | 4.1:+71338103 | None:intergenic |
| TAATGTCCTTGTTGATGAAA+GGG | 0.448219 | 4.1:-71339586 | MS.gene76702:CDS |
| TTCTTCTCAGGAACCAGCAC+AGG | 0.450915 | 4.1:+71337888 | None:intergenic |
| GGCAAGCCAGAGGCAAGCTC+TGG | 0.450969 | 4.1:-71335762 | MS.gene76702:CDS |
| CCCATTATAAGTTTCCCCTC+AGG | 0.451841 | 4.1:+71338252 | None:intergenic |
| TGCTGGTCGAATTGGTCGCT+TGG | 0.454425 | 4.1:-71339148 | MS.gene76702:CDS |
| AATGTGAGGGGTATGCAAGT+TGG | 0.455328 | 4.1:-71339870 | MS.gene76702:CDS |
| CTGATTCCTTCCTCGTCCTT+CGG | 0.456088 | 4.1:+71339422 | None:intergenic |
| AAAAGATTATCCAGTTCCCT+TGG | 0.457180 | 4.1:+71336826 | None:intergenic |
| CAACAAGACACCCTCGTCAT+AGG | 0.457379 | 4.1:-71335649 | MS.gene76702:CDS |
| CCAGAAGTCTTACCCTCTGC+TGG | 0.459866 | 4.1:-71339765 | MS.gene76702:CDS |
| AAAATGACGGGATGAGGTAC+TGG | 0.461362 | 4.1:-71339836 | MS.gene76702:CDS |
| ATAATGTCCTTGTTGATGAA+AGG | 0.463710 | 4.1:-71339587 | MS.gene76702:CDS |
| GGCCTTCTTCAGTCTCAAAA+GGG | 0.464157 | 4.1:-71338029 | MS.gene76702:CDS |
| TGGAAAGAAGAGTATATTTG+TGG | 0.465676 | 4.1:-71337235 | MS.gene76702:CDS |
| TGTTGCATGAACGTTGTTTG+TGG | 0.466630 | 4.1:-71342459 | MS.gene76702:CDS |
| CAGTTGCATAGACTGAATAC+AGG | 0.468777 | 4.1:+71338852 | None:intergenic |
| CCTGAGGGGAAACTTATAAT+GGG | 0.471341 | 4.1:-71338252 | MS.gene76702:CDS |
| GAGAATATACCTATATTAAG+TGG | 0.472374 | 4.1:-71338059 | MS.gene76702:CDS |
| GTGATTCAAATGGTTCATCT+AGG | 0.472455 | 4.1:-71339707 | MS.gene76702:CDS |
| AACAAATTCTGCTTCAGTAC+AGG | 0.472669 | 4.1:-71338217 | MS.gene76702:intron |
| AGGCCTCCTGCCGAAGGACG+AGG | 0.477953 | 4.1:-71339432 | MS.gene76702:CDS |
| TGTTTGCCCTTCCCACTTGG+TGG | 0.481303 | 4.1:+71335596 | None:intergenic |
| GTGGAACCTCGACGTCGATT+CGG | 0.481461 | 4.1:-71342440 | MS.gene76702:CDS |
| GCAAGTGATGCTGGTCGAAT+TGG | 0.484028 | 4.1:-71339156 | MS.gene76702:CDS |
| CAAGAGTTGCAGCAAATATC+GGG | 0.484696 | 4.1:-71339366 | MS.gene76702:CDS |
| TTCCCAGCAGTGCATATCTC+TGG | 0.485291 | 4.1:-71339904 | MS.gene76702:CDS |
| GGAGATGATGCAAGTGACTC+TGG | 0.485895 | 4.1:-71335692 | MS.gene76702:CDS |
| CCTCACAATGTTGATTTGGA+TGG | 0.486454 | 4.1:-71339807 | MS.gene76702:CDS |
| AGACACCCTCGTCATAGGCC+TGG | 0.487047 | 4.1:-71335644 | MS.gene76702:CDS |
| GACAGTCCTGAAGAGAATAG+TGG | 0.489705 | 4.1:-71335062 | MS.gene76702:CDS |
| TAGCCAGAGATATGCACTGC+TGG | 0.491104 | 4.1:+71339901 | None:intergenic |
| CGATTCGGTGGCGGAAAGGT+TGG | 0.493342 | 4.1:-71342425 | MS.gene76702:CDS |
| TATTGATAGCGAGGATGCAC+TGG | 0.493835 | 4.1:-71337826 | MS.gene76702:CDS |
| CCAAGTGGCTGATGCTGCCA+AGG | 0.498515 | 4.1:-71335336 | MS.gene76702:CDS |
| TTTGGCCAAATAATAATAGC+AGG | 0.500753 | 4.1:-71338900 | MS.gene76702:CDS |
| ATCAACAAGGACATTATGAA+AGG | 0.502025 | 4.1:+71339593 | None:intergenic |
| GAGACATACCTGAAGCAAAA+TGG | 0.503082 | 4.1:-71335212 | MS.gene76702:CDS |
| TTCAAGAAAGTGAATTGTGT+TGG | 0.503800 | 4.1:+71335027 | None:intergenic |
| GGCTAACCAATTAAATGTGA+GGG | 0.506229 | 4.1:-71339883 | MS.gene76702:CDS |
| TCACTTGAATGAGAACCATT+GGG | 0.509159 | 4.1:+71338104 | None:intergenic |
| GTTGTCTTTACCACCTGTGC+TGG | 0.513012 | 4.1:-71337901 | MS.gene76702:CDS |
| TGCTTCAGGTATGTCTCCAA+CGG | 0.513673 | 4.1:+71335218 | None:intergenic |
| GCTTCAGGTATGTCTCCAAC+GGG | 0.514701 | 4.1:+71335219 | None:intergenic |
| CTTCCTCGTCCTTCGGCAGG+AGG | 0.514830 | 4.1:+71339429 | None:intergenic |
| CAGTCTATGCAACTGCAAGC+TGG | 0.514895 | 4.1:-71338844 | MS.gene76702:CDS |
| CCTGGAGATAGAGTTGGCCT+AGG | 0.516685 | 4.1:+71339498 | None:intergenic |
| TGCAGTAACATTTAGCCGTA+TGG | 0.517779 | 4.1:-71338277 | MS.gene76702:intron |
| ATGCAGAAAATGACGGGATG+AGG | 0.517992 | 4.1:-71339842 | MS.gene76702:CDS |
| ACTTGAATTGCCAACCGGAT+CGG | 0.518323 | 4.1:-71335304 | MS.gene76702:CDS |
| AAATTCAGCTTCAAATGTGC+AGG | 0.519112 | 4.1:-71335159 | MS.gene76702:CDS |
| TTCTAGAGAGCGCATTTAGG+TGG | 0.520190 | 4.1:+71337994 | None:intergenic |
| CCCTCCTATCTCTCAACCTG+AGG | 0.521917 | 4.1:-71338980 | MS.gene76702:CDS |
| TAAATGCGCTCTCTAGAAAG+TGG | 0.523105 | 4.1:-71337989 | MS.gene76702:CDS |
| TTGTGTTTGCCCTTCCCACT+TGG | 0.523559 | 4.1:+71335593 | None:intergenic |
| ATTAGACTTGAATTGCCAAC+CGG | 0.524003 | 4.1:-71335309 | MS.gene76702:CDS |
| GCAAGATATATATGAATCAC+TGG | 0.525449 | 4.1:-71339661 | MS.gene76702:CDS |
| CCGATCTATGTGATAAGTGC+GGG | 0.529734 | 4.1:-71342380 | MS.gene76702:intron |
| ATGTAGAAATGCCTGAAAGC+AGG | 0.533071 | 4.1:-71337935 | MS.gene76702:CDS |
| AGGCCTTGCTATACGTATCT+GGG | 0.533919 | 4.1:+71339449 | None:intergenic |
| TCCTCGATCATGACAACACT+TGG | 0.535986 | 4.1:+71337744 | None:intergenic |
| GAGTGAGGGAAGACAAAATG+AGG | 0.539623 | 4.1:-71335120 | MS.gene76702:CDS |
| CTCCCAATGAATTGAATCCA+AGG | 0.539749 | 4.1:-71336843 | MS.gene76702:CDS |
| ACATAGATCGGCAAATTCAC+CGG | 0.541892 | 4.1:+71342392 | None:intergenic |
| TCAACAAGGACATTATGAAA+GGG | 0.542098 | 4.1:+71339594 | None:intergenic |
| ATGCTTAGTGCAAGTGATGC+TGG | 0.543355 | 4.1:-71339165 | MS.gene76702:CDS |
| CCTAGGCCAACTCTATCTCC+AGG | 0.543621 | 4.1:-71339498 | MS.gene76702:CDS |
| TTCTCCATACACGTCCACTT+AGG | 0.543699 | 4.1:+71336980 | None:intergenic |
| CAGTTGGTGCTGACATGGAT+TGG | 0.544785 | 4.1:-71337962 | MS.gene76702:CDS |
| ATTCAAGTCTAATTGTCCCT+TGG | 0.544992 | 4.1:+71335319 | None:intergenic |
| GGGAATTATGCAGAAAATGA+CGG | 0.547304 | 4.1:-71339849 | MS.gene76702:CDS |
| ATACGCAAAGGAAGGCCCTC+AGG | 0.550975 | 4.1:+71338964 | None:intergenic |
| GGAATTATGCAGAAAATGAC+GGG | 0.551880 | 4.1:-71339848 | MS.gene76702:CDS |
| CCTCGACGTCGATTCGGTGG+CGG | 0.551914 | 4.1:-71342434 | MS.gene76702:CDS |
| ATTTGGCAAAGGAGCGGTAA+GGG | 0.556170 | 4.1:+71339617 | None:intergenic |
| AGCACCCAGGCCTATGACGA+GGG | 0.556180 | 4.1:+71335639 | None:intergenic |
| GACACCCTCGTCATAGGCCT+GGG | 0.556520 | 4.1:-71335643 | MS.gene76702:CDS |
| CCATTATAAGTTTCCCCTCA+GGG | 0.557442 | 4.1:+71338253 | None:intergenic |
| AAGTCCTGAAGTACTTGCAC+AGG | 0.558364 | 4.1:+71340462 | None:intergenic |
| ATGATGCAAGTGACTCTGGC+AGG | 0.558412 | 4.1:-71335688 | MS.gene76702:CDS |
| GACAGGCAGCGGCAAGCCAG+AGG | 0.559302 | 4.1:-71335772 | MS.gene76702:CDS |
| GAACTCAGTTGGTGCTGACA+TGG | 0.559385 | 4.1:-71337967 | MS.gene76702:CDS |
| AAGATGTGAAAGGAAAAGAA+TGG | 0.562266 | 4.1:-71338939 | MS.gene76702:CDS |
| TTTCCGTCTAATATGCCCAA+TGG | 0.563491 | 4.1:-71338119 | MS.gene76702:CDS |
| GATCGGGAAGACATGCAAGC+TGG | 0.563741 | 4.1:-71335287 | MS.gene76702:CDS |
| GTTTCCCCTCAGGGTCCATA+CGG | 0.563865 | 4.1:+71338262 | None:intergenic |
| CTTGGCTTAACTTCTGGAGG+TGG | 0.565044 | 4.1:+71337762 | None:intergenic |
| AGAATTCAAGAAACAAAGAC+AGG | 0.565309 | 4.1:-71335789 | MS.gene76702:CDS |
| CCCGCACTTATCACATAGAT+CGG | 0.565675 | 4.1:+71342380 | None:intergenic |
| GCTGAAACTTACTTGGGAAG+AGG | 0.565759 | 4.1:-71337802 | MS.gene76702:CDS |
| TATCCAGCCACCAAGTGGGA+AGG | 0.566881 | 4.1:-71335603 | MS.gene76702:CDS |
| AAAGGTTGGATTCTTCGATC+CGG | 0.567319 | 4.1:-71342411 | MS.gene76702:CDS |
| ATCCAGCCACCAAGTGGGAA+GGG | 0.569945 | 4.1:-71335602 | MS.gene76702:CDS |
| TTCTCAGGAACCAGCACAGG+TGG | 0.570575 | 4.1:+71337891 | None:intergenic |
| CCTCCTATCTCTCAACCTGA+GGG | 0.572708 | 4.1:-71338979 | MS.gene76702:CDS |
| ACGTCGATTCGGTGGCGGAA+AGG | 0.575316 | 4.1:-71342429 | MS.gene76702:CDS |
| TCAAATGGTTCATCTAGGAC+TGG | 0.575345 | 4.1:-71339702 | MS.gene76702:CDS |
| ACTTATAATGGGGTTCAGGA+AGG | 0.575522 | 4.1:-71338241 | MS.gene76702:CDS |
| AGCCAGAGATATGCACTGCT+GGG | 0.576349 | 4.1:+71339902 | None:intergenic |
| GATTTGGCAAAGGAGCGGTA+AGG | 0.576714 | 4.1:+71339616 | None:intergenic |
| TGTCTTGTTGTGGTCAACAC+TGG | 0.577354 | 4.1:+71335662 | None:intergenic |
| TCTAGAAAGTGGAACTCAGT+TGG | 0.578317 | 4.1:-71337978 | MS.gene76702:CDS |
| GGAGATAGAGTTGGCCTAGG+AGG | 0.578335 | 4.1:+71339501 | None:intergenic |
| TCTAGAGAGCGCATTTAGGT+GGG | 0.579194 | 4.1:+71337995 | None:intergenic |
| TCCATGCAAAAGAATCTGGT+TGG | 0.579415 | 4.1:-71340673 | MS.gene76702:CDS |
| CAAGAAACAAAGACAGGCAG+CGG | 0.580250 | 4.1:-71335783 | MS.gene76702:CDS |
| TTCAAAGAGTGGCACAATGG+TGG | 0.580676 | 4.1:+71339191 | None:intergenic |
| GCATGTCTTCCCGATCCGGT+TGG | 0.581547 | 4.1:+71335294 | None:intergenic |
| CCTCAGGTTGAGAGATAGGA+GGG | 0.582109 | 4.1:+71338980 | None:intergenic |
| TCTAAGCCTGGAGATAGAGT+TGG | 0.583408 | 4.1:+71339492 | None:intergenic |
| GATCTAGACGCCAATAGAAG+TGG | 0.585121 | 4.1:+71339543 | None:intergenic |
| TATCTGCAGCGTCTCCATTG+TGG | 0.587990 | 4.1:-71340544 | MS.gene76702:intron |
| ATAGCAAGGCCTCCTGCCGA+AGG | 0.591462 | 4.1:-71339438 | MS.gene76702:CDS |
| ATACTCTTCTTTCCAAACAC+AGG | 0.593392 | 4.1:+71337243 | None:intergenic |
| GTCTGTATCCAGCCACCAAG+TGG | 0.593579 | 4.1:-71335608 | MS.gene76702:CDS |
| ATGACGAGGGTGTCTTGTTG+TGG | 0.595378 | 4.1:+71335652 | None:intergenic |
| CAGACATTACACGTGCATGT+AGG | 0.595381 | 4.1:+71335569 | None:intergenic |
| ACACTTGGCTTAACTTCTGG+AGG | 0.596735 | 4.1:+71337759 | None:intergenic |
| TTATGAAAGGGATTTGGCAA+AGG | 0.597001 | 4.1:+71339606 | None:intergenic |
| CCCTCAGGTTGAGAGATAGG+AGG | 0.598922 | 4.1:+71338979 | None:intergenic |
| ACAGTCCTGAAGAGAATAGT+GGG | 0.601508 | 4.1:-71335061 | MS.gene76702:CDS |
| TTCACATCTTGGATACGCAA+AGG | 0.602051 | 4.1:+71338952 | None:intergenic |
| TCCCAATGAATTGAATCCAA+GGG | 0.602257 | 4.1:-71336842 | MS.gene76702:CDS |
| TGGCTGGATACAGACAATAC+AGG | 0.604446 | 4.1:+71335616 | None:intergenic |
| GAGGTTGCTTATTGATAGCG+AGG | 0.605174 | 4.1:-71337835 | MS.gene76702:CDS |
| GAACCATTGGGCATATTAGA+CGG | 0.606117 | 4.1:+71338116 | None:intergenic |
| CATCTTGGATACGCAAAGGA+AGG | 0.606572 | 4.1:+71338956 | None:intergenic |
| CTCTTCTTTCCAAACACAGG+AGG | 0.606920 | 4.1:+71337246 | None:intergenic |
| CCATCCAAATCAACATTGTG+AGG | 0.607409 | 4.1:+71339807 | None:intergenic |
| TGGCTAACCAATTAAATGTG+AGG | 0.608102 | 4.1:-71339884 | MS.gene76702:CDS |
| TGAAGAAGCAAATCTCCCGT+TGG | 0.612037 | 4.1:-71335234 | MS.gene76702:CDS |
| CTCCATACACGTCCACTTAG+GGG | 0.615994 | 4.1:+71336982 | None:intergenic |
| TTGCGTATCCAAGATGTGAA+AGG | 0.616182 | 4.1:-71338949 | MS.gene76702:CDS |
| TCCATACACGTCCACTTAGG+GGG | 0.618210 | 4.1:+71336983 | None:intergenic |
| GAACCTCGACGTCGATTCGG+TGG | 0.622609 | 4.1:-71342437 | MS.gene76702:CDS |
| AGAGTGTACTTCATGTGGCA+AGG | 0.623087 | 4.1:-71340648 | MS.gene76702:intron |
| TTTGTTCCCTTTCATCAACA+AGG | 0.624287 | 4.1:+71339580 | None:intergenic |
| GAGCACCCAGGCCTATGACG+AGG | 0.624956 | 4.1:+71335638 | None:intergenic |
| GCTTCCAAATTCTCCAGCAG+AGG | 0.626276 | 4.1:+71339752 | None:intergenic |
| CTTCCAAATTCTCCAGCAGA+GGG | 0.626325 | 4.1:+71339753 | None:intergenic |
| ACAGACAACCAATGAGAGTG+AGG | 0.631032 | 4.1:-71335135 | MS.gene76702:CDS |
| TTCAAGTAACCAAGACCAAG+TGG | 0.631930 | 4.1:-71335351 | MS.gene76702:CDS |
| GCCGATCTATGTGATAAGTG+CGG | 0.642160 | 4.1:-71342381 | MS.gene76702:CDS |
| CTCCTGCCGAAGGACGAGGA+AGG | 0.643593 | 4.1:-71339428 | MS.gene76702:CDS |
| CTAGACGCCAATAGAAGTGG+AGG | 0.644014 | 4.1:+71339546 | None:intergenic |
| CAGCTTGAAATACTTGACAC+TGG | 0.646808 | 4.1:-71340502 | MS.gene76702:CDS |
| GGTTCAGAGAATGAAGCAAG+AGG | 0.647949 | 4.1:-71335389 | MS.gene76702:CDS |
| TTGAAGCAACACATCCACAA+TGG | 0.648024 | 4.1:+71340530 | None:intergenic |
| GTAATTCTGGGCCAATATCG+CGG | 0.648517 | 4.1:+71339393 | None:intergenic |
| TATTTGTGGTACGATCTACT+GGG | 0.648538 | 4.1:-71337221 | MS.gene76702:intron |
| TCTGTATCCAGCCACCAAGT+GGG | 0.649632 | 4.1:-71335607 | MS.gene76702:CDS |
| GCCAAGTGTTGTCATGATCG+AGG | 0.651574 | 4.1:-71337745 | MS.gene76702:CDS |
| AGTTGCATAGACTGAATACA+GGG | 0.651610 | 4.1:+71338853 | None:intergenic |
| ACTGGAGCTGAAACTTACTT+GGG | 0.656896 | 4.1:-71337808 | MS.gene76702:CDS |
| TTTCCCAGATACGTATAGCA+AGG | 0.657040 | 4.1:-71339452 | MS.gene76702:CDS |
| AACTGGAGTTAAATGTCGAG+AGG | 0.658373 | 4.1:+71335415 | None:intergenic |
| CTTAGAAGGAAACACAGGCA+TGG | 0.658844 | 4.1:-71339475 | MS.gene76702:CDS |
| CCAGGCTTAGAAGGAAACAC+AGG | 0.660663 | 4.1:-71339480 | MS.gene76702:CDS |
| CCGCCACCGAATCGACGTCG+AGG | 0.662296 | 4.1:+71342434 | None:intergenic |
| GTTCAGAGAATGAAGCAAGA+GGG | 0.662317 | 4.1:-71335388 | MS.gene76702:CDS |
| GCTAACCAATTAAATGTGAG+GGG | 0.663001 | 4.1:-71339882 | MS.gene76702:CDS |
| CAAGTGGCTGATGCTGCCAA+GGG | 0.663194 | 4.1:-71335335 | MS.gene76702:CDS |
| ACCACAGGATAAGTGAGCAG+TGG | 0.664291 | 4.1:-71337060 | MS.gene76702:intron |
| CTGAGGGGAAACTTATAATG+GGG | 0.667153 | 4.1:-71338251 | MS.gene76702:CDS |
| TCAGCAATCATGGAGGACCA+AGG | 0.667770 | 4.1:-71335459 | MS.gene76702:CDS |
| TCTGCATGACAGTTAAACGC+CGG | 0.670607 | 4.1:-71335550 | MS.gene76702:CDS |
| TTTAGCCGTATGGACCCTGA+GGG | 0.672269 | 4.1:-71338267 | MS.gene76702:CDS |
| AAAGGGATTTGGCAAAGGAG+CGG | 0.674141 | 4.1:+71339611 | None:intergenic |
| CAGACAACCAATGAGAGTGA+GGG | 0.686644 | 4.1:-71335134 | MS.gene76702:CDS |
| ATTTAGCCGTATGGACCCTG+AGG | 0.687200 | 4.1:-71338268 | MS.gene76702:CDS |
| TGATGCTTTAGCAAATGCTG+CGG | 0.695273 | 4.1:-71335720 | MS.gene76702:CDS |
| TTGCATAGACTGAATACAGG+GGG | 0.707100 | 4.1:+71338855 | None:intergenic |
| TGGAGAGAGTGTACTTCATG+TGG | 0.711681 | 4.1:-71340653 | MS.gene76702:CDS |
| CAGACAATACAGGAGCACCC+AGG | 0.725333 | 4.1:+71335626 | None:intergenic |
| CTTGAAATACTTGACACTGG+TGG | 0.733966 | 4.1:-71340499 | MS.gene76702:CDS |
| TTAGCCGTATGGACCCTGAG+GGG | 0.752430 | 4.1:-71338266 | MS.gene76702:CDS |
| GTTGCATAGACTGAATACAG+GGG | 0.770443 | 4.1:+71338854 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr4.1 | gene | 71335028 | 71342493 | 71335028 | ID=MS.gene76702 |
| chr4.1 | mRNA | 71335028 | 71342493 | 71335028 | ID=MS.gene76702.t1;Parent=MS.gene76702 |
| chr4.1 | exon | 71342381 | 71342493 | 71342381 | ID=MS.gene76702.t1.exon1;Parent=MS.gene76702.t1 |
| chr4.1 | CDS | 71342381 | 71342493 | 71342381 | ID=cds.MS.gene76702.t1;Parent=MS.gene76702.t1 |
| chr4.1 | exon | 71340649 | 71340730 | 71340649 | ID=MS.gene76702.t1.exon2;Parent=MS.gene76702.t1 |
| chr4.1 | CDS | 71340649 | 71340730 | 71340649 | ID=cds.MS.gene76702.t1;Parent=MS.gene76702.t1 |
| chr4.1 | exon | 71340456 | 71340557 | 71340456 | ID=MS.gene76702.t1.exon3;Parent=MS.gene76702.t1 |
| chr4.1 | CDS | 71340456 | 71340557 | 71340456 | ID=cds.MS.gene76702.t1;Parent=MS.gene76702.t1 |
| chr4.1 | exon | 71339363 | 71339982 | 71339363 | ID=MS.gene76702.t1.exon4;Parent=MS.gene76702.t1 |
| chr4.1 | CDS | 71339363 | 71339982 | 71339363 | ID=cds.MS.gene76702.t1;Parent=MS.gene76702.t1 |
| chr4.1 | exon | 71339125 | 71339221 | 71339125 | ID=MS.gene76702.t1.exon5;Parent=MS.gene76702.t1 |
| chr4.1 | CDS | 71339125 | 71339221 | 71339125 | ID=cds.MS.gene76702.t1;Parent=MS.gene76702.t1 |
| chr4.1 | exon | 71338836 | 71339010 | 71338836 | ID=MS.gene76702.t1.exon6;Parent=MS.gene76702.t1 |
| chr4.1 | CDS | 71338836 | 71339010 | 71338836 | ID=cds.MS.gene76702.t1;Parent=MS.gene76702.t1 |
| chr4.1 | exon | 71338218 | 71338294 | 71338218 | ID=MS.gene76702.t1.exon7;Parent=MS.gene76702.t1 |
| chr4.1 | CDS | 71338218 | 71338294 | 71338218 | ID=cds.MS.gene76702.t1;Parent=MS.gene76702.t1 |
| chr4.1 | exon | 71337722 | 71338147 | 71337722 | ID=MS.gene76702.t1.exon8;Parent=MS.gene76702.t1 |
| chr4.1 | CDS | 71337722 | 71338147 | 71337722 | ID=cds.MS.gene76702.t1;Parent=MS.gene76702.t1 |
| chr4.1 | exon | 71337222 | 71337271 | 71337222 | ID=MS.gene76702.t1.exon9;Parent=MS.gene76702.t1 |
| chr4.1 | CDS | 71337222 | 71337271 | 71337222 | ID=cds.MS.gene76702.t1;Parent=MS.gene76702.t1 |
| chr4.1 | exon | 71336962 | 71337075 | 71336962 | ID=MS.gene76702.t1.exon10;Parent=MS.gene76702.t1 |
| chr4.1 | CDS | 71336962 | 71337075 | 71336962 | ID=cds.MS.gene76702.t1;Parent=MS.gene76702.t1 |
| chr4.1 | exon | 71336812 | 71336879 | 71336812 | ID=MS.gene76702.t1.exon11;Parent=MS.gene76702.t1 |
| chr4.1 | CDS | 71336812 | 71336879 | 71336812 | ID=cds.MS.gene76702.t1;Parent=MS.gene76702.t1 |
| chr4.1 | exon | 71335028 | 71335809 | 71335028 | ID=MS.gene76702.t1.exon12;Parent=MS.gene76702.t1 |
| chr4.1 | CDS | 71335028 | 71335809 | 71335028 | ID=cds.MS.gene76702.t1;Parent=MS.gene76702.t1 |
| Gene Sequence |
| Protein sequence |
