Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene77478.t1 | AES71835.1 | 91.2 | 68 | 3 | 2 | 1 | 68 | 1 | 65 | 6.10E-22 | 112.8 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene77478.t1 | G7J9J6 | 91.2 | 68 | 3 | 2 | 1 | 68 | 1 | 65 | 4.4e-22 | 112.8 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene051064 | MS.gene77478 | 0.802057 | 6.72E-49 | -1.69E-46 |
| MS.gene052309 | MS.gene77478 | -0.813211 | 2.87E-51 | -1.69E-46 |
| MS.gene058498 | MS.gene77478 | 0.811277 | 7.59E-51 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene77478.t1 | MTR_3g083270 | 91.176 | 68 | 3 | 2 | 1 | 68 | 1 | 65 | 7.04e-36 | 115 |
| MS.gene77478.t1 | MTR_3g083285 | 91.176 | 68 | 3 | 2 | 1 | 68 | 1 | 65 | 7.04e-36 | 115 |
| MS.gene77478.t1 | MTR_3g083295 | 91.176 | 68 | 3 | 2 | 1 | 68 | 1 | 65 | 7.04e-36 | 115 |
| MS.gene77478.t1 | MTR_2g012480 | 70.968 | 62 | 12 | 3 | 1 | 62 | 1 | 56 | 5.91e-20 | 75.1 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 22 sgRNAs with CRISPR-Local
Find 20 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| AAAATCAACGGTGGAGTATT+AGG | 0.287872 | 3.2:+72096545 | MS.gene77478:CDS |
| ACCTGACCCCGCTTTGGAAT+CGG | 0.376203 | 3.2:-72096587 | None:intergenic |
| AGAAGGAGCAGCACATGATA+TGG | 0.377907 | 3.2:-72096666 | None:intergenic |
| TCCGATTCCAAAGCGGGGTC+AGG | 0.419316 | 3.2:+72096586 | MS.gene77478:CDS |
| ATCACCACCTGACCCCGCTT+TGG | 0.431061 | 3.2:-72096593 | None:intergenic |
| AAGCGGGGTCAGGTGGTGAT+TGG | 0.451258 | 3.2:+72096596 | MS.gene77478:CDS |
| TGACCGTCCGATTCCAAAGC+GGG | 0.457454 | 3.2:+72096580 | MS.gene77478:CDS |
| GTGGTGATTGGAATTGTGTT+GGG | 0.460629 | 3.2:+72096608 | MS.gene77478:CDS |
| TTACAAATTAAGCTAAAAGC+TGG | 0.468928 | 3.2:-72096697 | None:intergenic |
| GATTGGAATTGTGTTGGGAT+TGG | 0.472815 | 3.2:+72096613 | MS.gene77478:CDS |
| GGTGGTGATTGGAATTGTGT+TGG | 0.485203 | 3.2:+72096607 | MS.gene77478:CDS |
| AAAAGCTGGACTCTAGAAGA+AGG | 0.550483 | 3.2:-72096683 | None:intergenic |
| TGTGTTGGGATTGGCCAACT+CGG | 0.572810 | 3.2:+72096622 | MS.gene77478:CDS |
| GAAGATGCAAACAACCGAGT+TGG | 0.598645 | 3.2:-72096636 | None:intergenic |
| TCAACGGTGGAGTATTAGGA+AGG | 0.608387 | 3.2:+72096549 | MS.gene77478:CDS |
| GATTCCAAAGCGGGGTCAGG+TGG | 0.610196 | 3.2:+72096589 | MS.gene77478:CDS |
| CAAAGCAAAGAGAAGATGGA+GGG | 0.621656 | 3.2:+72096491 | None:intergenic |
| ACTGCAATCATGAAAATCAA+CGG | 0.647400 | 3.2:+72096533 | MS.gene77478:CDS |
| GACCCCGCTTTGGAATCGGA+CGG | 0.688444 | 3.2:-72096583 | None:intergenic |
| GCAATCATGAAAATCAACGG+TGG | 0.699924 | 3.2:+72096536 | MS.gene77478:CDS |
| GACCGTCCGATTCCAAAGCG+GGG | 0.710779 | 3.2:+72096581 | MS.gene77478:CDS |
| GTGACCGTCCGATTCCAAAG+CGG | 0.721311 | 3.2:+72096579 | MS.gene77478:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| ACTGCAATCATGAAAATCAA+CGG | + | chr3.2:72096533-72096552 | MS.gene77478:CDS | 30.0% | |
| ! | AAAATCAACGGTGGAGTATT+AGG | + | chr3.2:72096545-72096564 | MS.gene77478:CDS | 35.0% |
| GATTGGAATTGTGTTGGGAT+TGG | + | chr3.2:72096613-72096632 | MS.gene77478:CDS | 40.0% | |
| GCAATCATGAAAATCAACGG+TGG | + | chr3.2:72096536-72096555 | MS.gene77478:CDS | 40.0% | |
| GTGGTGATTGGAATTGTGTT+GGG | + | chr3.2:72096608-72096627 | MS.gene77478:CDS | 40.0% | |
| ! | AAAAGCTGGACTCTAGAAGA+AGG | - | chr3.2:72096686-72096705 | None:intergenic | 40.0% |
| AGAAGGAGCAGCACATGATA+TGG | - | chr3.2:72096669-72096688 | None:intergenic | 45.0% | |
| GAAGATGCAAACAACCGAGT+TGG | - | chr3.2:72096639-72096658 | None:intergenic | 45.0% | |
| GGTGGTGATTGGAATTGTGT+TGG | + | chr3.2:72096607-72096626 | MS.gene77478:CDS | 45.0% | |
| ! | TCAACGGTGGAGTATTAGGA+AGG | + | chr3.2:72096549-72096568 | MS.gene77478:CDS | 45.0% |
| !! | TGTGTTGGGATTGGCCAACT+CGG | + | chr3.2:72096622-72096641 | MS.gene77478:CDS | 50.0% |
| GTGACCGTCCGATTCCAAAG+CGG | + | chr3.2:72096579-72096598 | MS.gene77478:CDS | 55.0% | |
| TGACCGTCCGATTCCAAAGC+GGG | + | chr3.2:72096580-72096599 | MS.gene77478:CDS | 55.0% | |
| !! | ACCTGACCCCGCTTTGGAAT+CGG | - | chr3.2:72096590-72096609 | None:intergenic | 55.0% |
| AAGCGGGGTCAGGTGGTGAT+TGG | + | chr3.2:72096596-72096615 | MS.gene77478:CDS | 60.0% | |
| ATCACCACCTGACCCCGCTT+TGG | - | chr3.2:72096596-72096615 | None:intergenic | 60.0% | |
| GACCGTCCGATTCCAAAGCG+GGG | + | chr3.2:72096581-72096600 | MS.gene77478:CDS | 60.0% | |
| GATTCCAAAGCGGGGTCAGG+TGG | + | chr3.2:72096589-72096608 | MS.gene77478:CDS | 60.0% | |
| TCCGATTCCAAAGCGGGGTC+AGG | + | chr3.2:72096586-72096605 | MS.gene77478:CDS | 60.0% | |
| !! | GACCCCGCTTTGGAATCGGA+CGG | - | chr3.2:72096586-72096605 | None:intergenic | 60.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr3.2 | gene | 72096506 | 72096712 | 72096506 | ID=MS.gene77478 |
| chr3.2 | mRNA | 72096506 | 72096712 | 72096506 | ID=MS.gene77478.t1;Parent=MS.gene77478 |
| chr3.2 | exon | 72096506 | 72096712 | 72096506 | ID=MS.gene77478.t1.exon1;Parent=MS.gene77478.t1 |
| chr3.2 | CDS | 72096506 | 72096712 | 72096506 | ID=cds.MS.gene77478.t1;Parent=MS.gene77478.t1 |
| Gene Sequence |
| Protein sequence |
