Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene77577.t1 | XP_003601753.1 | 98.6 | 69 | 1 | 0 | 1 | 69 | 1 | 69 | 5.80E-28 | 132.9 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene77577.t1 | G7JBE9 | 98.6 | 69 | 1 | 0 | 1 | 69 | 1 | 69 | 4.2e-28 | 132.9 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI:
| Gene1 | Gene2 | Type |
|---|---|---|
| MS.gene90025 | MS.gene77577 | PPI |
| MS.gene98091 | MS.gene77577 | PPI |
| MS.gene000435 | MS.gene77577 | PPI |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene77577.t1 | MTR_3g085020 | 98.551 | 69 | 1 | 0 | 1 | 69 | 1 | 69 | 8.60e-43 | 132 |
| MS.gene77577.t1 | MTR_8g096840 | 84.058 | 69 | 11 | 0 | 1 | 69 | 1 | 69 | 2.00e-37 | 119 |
| MS.gene77577.t1 | MTR_7g028975 | 65.217 | 69 | 24 | 0 | 1 | 69 | 1 | 69 | 2.57e-31 | 103 |
| MS.gene77577.t1 | MTR_8g097020 | 62.319 | 69 | 26 | 0 | 1 | 69 | 1 | 69 | 2.46e-30 | 101 |
| MS.gene77577.t1 | MTR_7g028870 | 65.152 | 66 | 23 | 0 | 2 | 67 | 3 | 68 | 9.16e-29 | 97.8 |
| MS.gene77577.t1 | MTR_8g027820 | 62.319 | 69 | 26 | 0 | 1 | 69 | 1 | 69 | 1.84e-28 | 98.2 |
| MS.gene77577.t1 | MTR_3g085030 | 59.420 | 69 | 28 | 0 | 1 | 69 | 1 | 69 | 5.59e-27 | 93.2 |
| MS.gene77577.t1 | MTR_8g097030 | 77.941 | 68 | 15 | 0 | 2 | 69 | 3 | 70 | 5.63e-26 | 90.5 |
| MS.gene77577.t1 | MTR_7g028940 | 57.971 | 69 | 29 | 0 | 1 | 69 | 1 | 69 | 1.82e-24 | 86.7 |
| MS.gene77577.t1 | MTR_7g028815 | 67.273 | 55 | 18 | 0 | 1 | 55 | 1 | 55 | 7.05e-23 | 84.3 |
| MS.gene77577.t1 | MTR_7g028945 | 56.863 | 51 | 22 | 0 | 1 | 51 | 1 | 51 | 1.58e-16 | 66.6 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene77577.t1 | AT3G48660 | 81.159 | 69 | 13 | 0 | 1 | 69 | 1 | 69 | 1.70e-35 | 115 |
| MS.gene77577.t1 | AT5G63500 | 78.261 | 69 | 15 | 0 | 1 | 69 | 1 | 69 | 3.05e-33 | 108 |
| MS.gene77577.t1 | AT5G08391 | 62.319 | 69 | 26 | 0 | 1 | 69 | 1 | 69 | 2.61e-30 | 101 |
| MS.gene77577.t1 | AT3G27027 | 66.154 | 65 | 22 | 0 | 3 | 67 | 4 | 68 | 2.40e-28 | 96.7 |
| MS.gene77577.t1 | AT3G01950 | 61.194 | 67 | 26 | 0 | 3 | 69 | 4 | 70 | 5.17e-26 | 90.9 |
| MS.gene77577.t1 | AT5G40980 | 70.909 | 55 | 16 | 0 | 2 | 56 | 3 | 57 | 1.04e-25 | 90.1 |
| MS.gene77577.t1 | AT3G27030 | 62.121 | 66 | 25 | 0 | 1 | 66 | 1 | 66 | 2.44e-25 | 91.3 |
| MS.gene77577.t1 | AT3G27030 | 57.971 | 69 | 29 | 0 | 1 | 69 | 74 | 142 | 1.51e-24 | 89.4 |
| MS.gene77577.t1 | AT5G14110 | 57.812 | 64 | 27 | 0 | 3 | 66 | 4 | 67 | 4.59e-24 | 85.9 |
| MS.gene77577.t1 | AT3G01940 | 72.727 | 66 | 18 | 0 | 2 | 67 | 4 | 69 | 1.44e-23 | 84.7 |
| MS.gene77577.t1 | AT5G50560 | 62.903 | 62 | 23 | 0 | 1 | 62 | 1 | 62 | 4.92e-18 | 70.5 |
| MS.gene77577.t1 | AT5G50660 | 62.903 | 62 | 23 | 0 | 1 | 62 | 1 | 62 | 4.92e-18 | 70.5 |
| MS.gene77577.t1 | AT5G40960 | 45.455 | 66 | 36 | 0 | 1 | 66 | 1 | 66 | 1.04e-12 | 57.0 |
Find 19 sgRNAs with CRISPR-Local
Find 22 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| AACCAGTGGTGTGTCTATTT+TGG | 0.273169 | 3.2:-73098577 | MS.gene77577:CDS |
| AGAAACAAAGTGGTGGAGTT+TGG | 0.286192 | 3.2:-73098609 | MS.gene77577:CDS |
| TCCACCACTTTGTTTCTTCC+AGG | 0.330714 | 3.2:+73098615 | None:intergenic |
| ACCATCTTTCTTATTGCCAT+TGG | 0.390535 | 3.2:-73098525 | MS.gene77577:CDS |
| GAAACAAAGTGGTGGAGTTT+GGG | 0.392874 | 3.2:-73098608 | MS.gene77577:CDS |
| CTGTTTGTGTTGCTAAGTCC+AGG | 0.405516 | 3.2:-73098654 | MS.gene77577:CDS |
| GGGTTGCTGTTTCAACTGCC+TGG | 0.426401 | 3.2:-73098633 | MS.gene77577:CDS |
| ATTGGTGTGCATATCAATAC+TGG | 0.488306 | 3.2:-73098507 | MS.gene77577:CDS |
| ACCAATGGCAATAAGAAAGA+TGG | 0.521238 | 3.2:+73098524 | None:intergenic |
| GGCAGTTGAAACAGCAACCC+TGG | 0.523057 | 3.2:+73098636 | None:intergenic |
| AGCACCACTGCTATTACCAC+TGG | 0.541172 | 3.2:+73098675 | None:intergenic |
| TGTTTGTGTTGCTAAGTCCA+GGG | 0.571919 | 3.2:-73098653 | MS.gene77577:CDS |
| GACCAAAATAGACACACCAC+TGG | 0.627960 | 3.2:+73098575 | None:intergenic |
| GGGTCCAGTGGTAATAGCAG+TGG | 0.638939 | 3.2:-73098679 | MS.gene77577:CDS |
| AGTATTGATATGCACACCAA+TGG | 0.648336 | 3.2:+73098509 | None:intergenic |
| GCCTGGAAGAAACAAAGTGG+TGG | 0.658875 | 3.2:-73098616 | MS.gene77577:CDS |
| AATGGCGGATTGGGGTCCAG+TGG | 0.665904 | 3.2:-73098691 | MS.gene77577:CDS |
| TTTGGGAACATGCAAACCAG+TGG | 0.682075 | 3.2:-73098591 | MS.gene77577:CDS |
| ACTGCCTGGAAGAAACAAAG+TGG | 0.704719 | 3.2:-73098619 | MS.gene77577:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| AATGAGTCCAAAAAAGAGGA+TGG | + | chr3.2:73098648-73098667 | None:intergenic | 35.0% | |
| ACCAATGGCAATAAGAAAGA+TGG | + | chr3.2:73098672-73098691 | None:intergenic | 35.0% | |
| ACCATCTTTCTTATTGCCAT+TGG | - | chr3.2:73098668-73098687 | MS.gene77577:CDS | 35.0% | |
| ATTGGTGTGCATATCAATAC+TGG | - | chr3.2:73098686-73098705 | MS.gene77577:CDS | 35.0% | |
| ! | TGGTAATGAGTCCAAAAAAG+AGG | + | chr3.2:73098652-73098671 | None:intergenic | 35.0% |
| !! | AGTATTGATATGCACACCAA+TGG | + | chr3.2:73098687-73098706 | None:intergenic | 35.0% |
| GTCCAAAAAAGAGGATGGTA+TGG | + | chr3.2:73098643-73098662 | None:intergenic | 40.0% | |
| ! | AACCAGTGGTGTGTCTATTT+TGG | - | chr3.2:73098616-73098635 | MS.gene77577:CDS | 40.0% |
| ! | AGAAACAAAGTGGTGGAGTT+TGG | - | chr3.2:73098584-73098603 | MS.gene77577:CDS | 40.0% |
| ! | GAAACAAAGTGGTGGAGTTT+GGG | - | chr3.2:73098585-73098604 | MS.gene77577:CDS | 40.0% |
| ! | TGTTTGTGTTGCTAAGTCCA+GGG | - | chr3.2:73098540-73098559 | MS.gene77577:CDS | 40.0% |
| !!! | GTCCATACCATCCTCTTTTT+TGG | - | chr3.2:73098638-73098657 | MS.gene77577:CDS | 40.0% |
| ACTGCCTGGAAGAAACAAAG+TGG | - | chr3.2:73098574-73098593 | MS.gene77577:CDS | 45.0% | |
| GACCAAAATAGACACACCAC+TGG | + | chr3.2:73098621-73098640 | None:intergenic | 45.0% | |
| TTTGGGAACATGCAAACCAG+TGG | - | chr3.2:73098602-73098621 | MS.gene77577:CDS | 45.0% | |
| ! | CTGTTTGTGTTGCTAAGTCC+AGG | - | chr3.2:73098539-73098558 | MS.gene77577:CDS | 45.0% |
| ! | TCCACCACTTTGTTTCTTCC+AGG | + | chr3.2:73098581-73098600 | None:intergenic | 45.0% |
| AGCACCACTGCTATTACCAC+TGG | + | chr3.2:73098521-73098540 | None:intergenic | 50.0% | |
| GCCTGGAAGAAACAAAGTGG+TGG | - | chr3.2:73098577-73098596 | MS.gene77577:CDS | 50.0% | |
| GGCAGTTGAAACAGCAACCC+TGG | + | chr3.2:73098560-73098579 | None:intergenic | 55.0% | |
| GGGTCCAGTGGTAATAGCAG+TGG | - | chr3.2:73098514-73098533 | MS.gene77577:CDS | 55.0% | |
| !! | GGGTTGCTGTTTCAACTGCC+TGG | - | chr3.2:73098560-73098579 | MS.gene77577:CDS | 55.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr3.2 | gene | 73098503 | 73098712 | 73098503 | ID=MS.gene77577 |
| chr3.2 | mRNA | 73098503 | 73098712 | 73098503 | ID=MS.gene77577.t1;Parent=MS.gene77577 |
| chr3.2 | exon | 73098503 | 73098712 | 73098503 | ID=MS.gene77577.t1.exon1;Parent=MS.gene77577.t1 |
| chr3.2 | CDS | 73098503 | 73098712 | 73098503 | ID=cds.MS.gene77577.t1;Parent=MS.gene77577.t1 |
| Gene Sequence |
| Protein sequence |
