AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene77612

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene77612.t1 MTR_2g066130 98.005 401 8 0 1 401 1 401 0.0 785
MS.gene77612.t1 MTR_2g066120 97.756 401 9 0 1 401 1 401 0.0 783
MS.gene77612.t1 MTR_2g066110 86.181 398 55 0 4 401 77 474 0.0 690
MS.gene77612.t1 MTR_1242s0010 97.788 226 5 0 176 401 1 226 7.97e-155 436
MS.gene77612.t1 MTR_1680s0010 48.792 207 99 3 15 219 148 349 4.75e-54 184
MS.gene77612.t1 MTR_6g018680 31.514 403 257 9 7 401 82 473 4.21e-45 166
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene77612.t1 AT1G79550 88.279 401 47 0 1 401 1 401 0.0 717
MS.gene77612.t1 AT1G79550 88.279 401 47 0 1 401 1 401 0.0 717
MS.gene77612.t1 AT1G56190 84.422 398 62 0 4 401 76 473 0.0 684
MS.gene77612.t1 AT3G12780 85.427 398 58 0 4 401 79 476 0.0 683
MS.gene77612.t1 AT1G56190 84.422 398 62 0 4 401 3 400 0.0 682

Find 116 sgRNAs with CRISPR-Local

Find 196 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GCCAACTTCTTTGCAAATTC+AGG 0.212968 2.1:-30543461 None:intergenic
TGATCTTGCAACTTCACTTA+TGG 0.256353 2.1:+30544074 MS.gene77612:CDS
TACTTGAAGCCCTCTGTTGC+AGG 0.303421 2.1:+30543563 MS.gene77612:CDS
AATAGCAGCAAATGGTTTCT+TGG 0.312833 2.1:-30543899 None:intergenic
CCAAGGTTGTCTGAACTTCT+TGG 0.315943 2.1:+30543199 MS.gene77612:CDS
CTGTAGAAGCATGAGCTCTA+TGG 0.325002 2.1:-30543526 None:intergenic
CTTGTCCAATGCTTCATTGA+AGG 0.339632 2.1:-30544517 None:intergenic
GGATGGATGGGATTGGATAT+TGG 0.342634 2.1:+30544477 MS.gene77612:CDS
GAAGTTGGTTGCACAAATTC+CGG 0.354219 2.1:+30543379 MS.gene77612:CDS
TACTTAGGTGTTACACCCTT+TGG 0.355902 2.1:-30543157 None:intergenic
ATAGCAGCAAATGGTTTCTT+GGG 0.363393 2.1:-30543898 None:intergenic
GGTTGATCTAAATGTTCCTT+TGG 0.363594 2.1:+30542528 MS.gene77612:CDS
ATAACCCTGAGCCTTGTAAA+AGG 0.373147 2.1:-30544016 None:intergenic
CTCTGCCAATTTCTTAGCTA+TGG 0.377521 2.1:-30544913 None:intergenic
GCAGGCCATAGCTAAGAAAT+TGG 0.378175 2.1:+30544908 MS.gene77612:intron
CATAAGGAACCCTGCAACAG+AGG 0.384154 2.1:-30543573 None:intergenic
ATCAAGTACTTGACTGGTTA+TGG 0.390517 2.1:+30542604 MS.gene77612:CDS
CTTTACGTGAATGATGCTTT+TGG 0.395928 2.1:+30543497 MS.gene77612:CDS
CCATTTGCTGCTATTGTTGG+TGG 0.404872 2.1:+30543907 MS.gene77612:CDS
CGGAGTTATTGAATCCTTGT+TGG 0.406022 2.1:+30543951 MS.gene77612:CDS
CCACATCAGTTGGAAGCAAA+AGG 0.407116 2.1:-30544122 None:intergenic
CATTCTTCTCTTCTTCCTTG+TGG 0.413146 2.1:-30543436 None:intergenic
ATTGAAAGAAGCTGAATTGA+AGG 0.413514 2.1:+30542486 MS.gene77612:CDS
TGCCATTGGGTCCTCTCTTG+TGG 0.416339 2.1:+30544038 MS.gene77612:CDS
GACATTCTCTTGCTTGGTGG+AGG 0.425395 2.1:+30543982 MS.gene77612:CDS
TCCAATCCCATCCATCCGTC+AGG 0.431077 2.1:-30544471 None:intergenic
TGGTGAGGAAGTTGAGAAGT+TGG 0.433027 2.1:+30543364 MS.gene77612:CDS
AAAGTTGACATTCTCTTGCT+TGG 0.435274 2.1:+30543976 MS.gene77612:CDS
AGTCATCCTTTCTAGTCATC+TGG 0.440297 2.1:+30542633 MS.gene77612:CDS
CAGGAGCTTGACTATCTAGT+TGG 0.444679 2.1:+30543862 MS.gene77612:intron
CCCTACATACCTTCTGCATA+AGG 0.445083 2.1:-30543589 None:intergenic
GGATTCAATAACTCCGATCT+TGG 0.447530 2.1:-30543944 None:intergenic
GTTGACATTCTCTTGCTTGG+TGG 0.459365 2.1:+30543979 MS.gene77612:CDS
AAGGCTCAGGGTTATGCCAT+TGG 0.462386 2.1:+30544024 MS.gene77612:CDS
TCATCAAGAGCAAGGACTCC+GGG 0.473099 2.1:-30545077 None:intergenic
AAACCATTTGCTGCTATTGT+TGG 0.476296 2.1:+30543904 MS.gene77612:CDS
GCCTGAATTTGCAAAGAAGT+TGG 0.479280 2.1:+30543460 MS.gene77612:CDS
AGAAGCAAAACACCACCTTC+CGG 0.481625 2.1:-30543398 None:intergenic
AAGATGAGTCACATCTCAAC+TGG 0.482513 2.1:+30545014 MS.gene77612:CDS
AGCTGAATTGAAGGGAAAGA+GGG 0.483862 2.1:+30542495 MS.gene77612:CDS
AAGGGAAACTCCTTTCGCCT+TGG 0.487168 2.1:-30544103 None:intergenic
AGGCTCAGGGTTATGCCATT+GGG 0.487497 2.1:+30544025 MS.gene77612:CDS
GCCAGCTTAGAGCTTCTTGA+GGG 0.493948 2.1:+30545044 MS.gene77612:CDS
TGCTATTGTTGGTGGCTCAA+AGG 0.497576 2.1:+30543915 MS.gene77612:CDS
TGTAGAAGCATGAGCTCTAT+GGG 0.498596 2.1:-30543525 None:intergenic
CCAGTCAAGTACTTGATAGT+AGG 0.499553 2.1:-30542598 None:intergenic
ACAAGAGTCAGACCATCATA+TGG 0.501196 2.1:+30544535 MS.gene77612:CDS
ATCATCAAGAGCAAGGACTC+CGG 0.502112 2.1:-30545078 None:intergenic
CCCTGACGGATGGATGGGAT+TGG 0.504660 2.1:+30544470 MS.gene77612:CDS
AAGAAATTGGCAGAGGTAAG+TGG 0.507070 2.1:+30544921 MS.gene77612:CDS
CCTACTATCAAGTACTTGAC+TGG 0.509296 2.1:+30542598 MS.gene77612:CDS
AAGCTGAATTGAAGGGAAAG+AGG 0.513423 2.1:+30542494 MS.gene77612:CDS
AAGTATCCCTGACGGATGGA+TGG 0.514673 2.1:+30544464 MS.gene77612:CDS
CCCCTCAAGAAGCTCTAAGC+TGG 0.515261 2.1:-30545045 None:intergenic
AAGGGAGTAACAACCATCAT+TGG 0.515661 2.1:+30544945 MS.gene77612:CDS
GTCTGAACTTCTTGGAACTC+AGG 0.517487 2.1:+30543207 MS.gene77612:CDS
AGAGCAAGGACTCCGGGGAG+TGG 0.520690 2.1:-30545071 None:intergenic
CAAAACCTTCAATGAAGCAT+TGG 0.522167 2.1:+30544512 MS.gene77612:CDS
ATCATATGGAATGGACCAAT+GGG 0.524600 2.1:+30544549 MS.gene77612:CDS
CACATCAGTTGGAAGCAAAA+GGG 0.527551 2.1:-30544121 None:intergenic
GAGTTCGACAAGTTTGCAGC+GGG 0.528899 2.1:+30544579 MS.gene77612:CDS
TAATACCTTGTTGTTAGCAT+CGG 0.532576 2.1:-30544166 None:intergenic
CATATACCAGATGACTAGAA+AGG 0.534558 2.1:-30542639 None:intergenic
CATTGGTCCATTCCATATGA+TGG 0.537194 2.1:-30544547 None:intergenic
AGAGGCTTCAAACTGTACTT+AGG 0.544386 2.1:-30543172 None:intergenic
TCAAAGGTGTCTTCCAAGAT+CGG 0.545377 2.1:+30543931 MS.gene77612:CDS
AAGATTGCTGATGACTGTAT+TGG 0.545548 2.1:+30543344 MS.gene77612:CDS
CTTGAGGGGAAGCCACTCCC+CGG 0.547185 2.1:+30545059 MS.gene77612:CDS
CCACCAACAATAGCAGCAAA+TGG 0.547345 2.1:-30543907 None:intergenic
GTAGCAGCAGTCGAGAAGGT+TGG 0.552309 2.1:+30544981 MS.gene77612:CDS
CATCATATGGAATGGACCAA+TGG 0.557291 2.1:+30544548 MS.gene77612:CDS
ATTGGCAGAGGTAAGTGGCA+AGG 0.559148 2.1:+30544926 MS.gene77612:CDS
AGAGTCGCCACCACCAATGA+TGG 0.560271 2.1:-30544958 None:intergenic
CTCTGTAGCAGCAGTCGAGA+AGG 0.564703 2.1:+30544977 MS.gene77612:CDS
TGGTTTCTTGGGGTTCGACA+CGG 0.564821 2.1:-30543887 None:intergenic
TGCCAGCTTAGAGCTTCTTG+AGG 0.565118 2.1:+30545043 MS.gene77612:CDS
GTTTGAAGCCTCTTGTACCA+AGG 0.565504 2.1:+30543182 MS.gene77612:CDS
TGCAGGGTTCCTTATGCAGA+AGG 0.565829 2.1:+30543580 MS.gene77612:CDS
AGTATCCCTGACGGATGGAT+GGG 0.566535 2.1:+30544465 MS.gene77612:CDS
ACTTGAAGCCCTCTGTTGCA+GGG 0.572918 2.1:+30543564 MS.gene77612:CDS
AGAGCTCATGCTTCTACAGA+AGG 0.574431 2.1:+30543530 MS.gene77612:CDS
AGTCAGACCATCATATGGAA+TGG 0.580523 2.1:+30544540 MS.gene77612:CDS
TCAGACAACCTTGGTACAAG+AGG 0.582460 2.1:-30543190 None:intergenic
CATTGGGTCCTCTCTTGTGG+AGG 0.584637 2.1:+30544041 MS.gene77612:CDS
GTTGCACAAATTCCGGAAGG+TGG 0.588779 2.1:+30543386 MS.gene77612:CDS
TTGGCAGAGGTAAGTGGCAA+GGG 0.589578 2.1:+30544927 MS.gene77612:CDS
TTGAAAGAAGCTGAATTGAA+GGG 0.590124 2.1:+30542487 MS.gene77612:CDS
TCAGCGACGACCACATCAGT+TGG 0.590993 2.1:-30544132 None:intergenic
CCGTCAGGGATACTTGATGA+TGG 0.594879 2.1:-30544456 None:intergenic
GTAACAACCATCATTGGTGG+TGG 0.602820 2.1:+30544951 MS.gene77612:CDS
GGAGTAACAACCATCATTGG+TGG 0.602916 2.1:+30544948 MS.gene77612:CDS
ATAAGGAACCCTGCAACAGA+GGG 0.610172 2.1:-30543572 None:intergenic
CTTGTCTTCCTCCACAAGAG+AGG 0.611431 2.1:-30544049 None:intergenic
AGTCACATCTCAACTGGTGG+AGG 0.611878 2.1:+30545020 MS.gene77612:CDS
ATGAGTCACATCTCAACTGG+TGG 0.612720 2.1:+30545017 MS.gene77612:CDS
TAGCAGCAAATGGTTTCTTG+GGG 0.613973 2.1:-30543897 None:intergenic
TTTAAGCATCATCAAGAGCA+AGG 0.617415 2.1:-30545085 None:intergenic
CATAGCTAAGAAATTGGCAG+AGG 0.620163 2.1:+30544914 MS.gene77612:CDS
CATCAAGTATCCCTGACGGA+TGG 0.622098 2.1:+30544460 MS.gene77612:CDS
AATGTCAACTTTCTCCAACA+AGG 0.622415 2.1:-30543965 None:intergenic
CCAAGAAGTTCAGACAACCT+TGG 0.623502 2.1:-30543199 None:intergenic
CTCCACAAGAGAGGACCCAA+TGG 0.625121 2.1:-30544040 None:intergenic
CCAATCCCATCCATCCGTCA+GGG 0.633454 2.1:-30544470 None:intergenic
CAAGTTTGCAGCGGGAACAG+AGG 0.638802 2.1:+30544587 MS.gene77612:CDS
CCATCATCAAGTATCCCTGA+CGG 0.643206 2.1:+30544456 MS.gene77612:CDS
ATATTCAAATTATCATCCAA+AGG 0.645495 2.1:-30542544 None:intergenic
TTGGTTGCACAAATTCCGGA+AGG 0.648276 2.1:+30543383 MS.gene77612:CDS
CCAGCTTAGAGCTTCTTGAG+GGG 0.656655 2.1:+30545045 MS.gene77612:CDS
ATTCACGTAAAGATCAGCAA+GGG 0.665058 2.1:-30543486 None:intergenic
TGAGTTCGACAAGTTTGCAG+CGG 0.665740 2.1:+30544578 MS.gene77612:CDS
TGCTGCCGATGCTAACAACA+AGG 0.671642 2.1:+30544161 MS.gene77612:CDS
CATTCACGTAAAGATCAGCA+AGG 0.675920 2.1:-30543487 None:intergenic
TGCTGATGACTGTATTGGTG+AGG 0.677716 2.1:+30543349 MS.gene77612:CDS
TGATAGTAGGAACAGCAGCA+CGG 0.682054 2.1:-30542585 None:intergenic
CATCAAGAGCAAGGACTCCG+GGG 0.688602 2.1:-30545076 None:intergenic
AGAGAATGTGAGGTTCCACA+AGG 0.692461 2.1:+30543421 MS.gene77612:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! ATTATTAACTTATTACACAA+AGG - chr2.1:30543297-30543316 None:intergenic 15.0%
!! TAATGAGATAATGATATTTA+TGG + chr2.1:30542765-30542784 MS.gene77612:intron 15.0%
!! TACAAAATAATGTCTAATAA+CGG + chr2.1:30543727-30543746 MS.gene77612:intron 15.0%
!!! TTATTAGTTTAATTGTTATG+TGG + chr2.1:30542854-30542873 MS.gene77612:intron 15.0%
!! AATGGTGAATGATTTATTAA+TGG - chr2.1:30544661-30544680 None:intergenic 20.0%
!! ATAAATATTGCTAATTGTTC+TGG + chr2.1:30543690-30543709 MS.gene77612:intron 20.0%
!! ATATTCAAATTATCATCCAA+AGG - chr2.1:30542547-30542566 None:intergenic 20.0%
!! TAAATATTGCTAATTGTTCT+GGG + chr2.1:30543691-30543710 MS.gene77612:intron 20.0%
!! TTGTGATTTCGTTATATAAT+TGG + chr2.1:30543617-30543636 MS.gene77612:intron 20.0%
!!! TATGATTTATACCTTTTACA+AGG + chr2.1:30544005-30544024 MS.gene77612:CDS 20.0%
!!! TTACTTATTTGATTTTTCGA+AGG + chr2.1:30542904-30542923 MS.gene77612:intron 20.0%
!!! TTTTGCATAACATTCAATAA+TGG - chr2.1:30544679-30544698 None:intergenic 20.0%
! AAAAATAAGTCAATCCAAAC+GGG - chr2.1:30542967-30542986 None:intergenic 25.0%
! ATGAAGAGATAAAGAAACTA+AGG + chr2.1:30542940-30542959 MS.gene77612:intron 25.0%
! CAAAAATAAGTCAATCCAAA+CGG - chr2.1:30542968-30542987 None:intergenic 25.0%
! TATAACCAAGAATAATCAAC+AGG - chr2.1:30543320-30543339 None:intergenic 25.0%
! TGAAGAGATAAAGAAACTAA+GGG + chr2.1:30542941-30542960 MS.gene77612:intron 25.0%
!! ATAATCCTGTTGATTATTCT+TGG + chr2.1:30543312-30543331 MS.gene77612:intron 25.0%
!! TGTTGATTATTCTTGGTTAT+AGG + chr2.1:30543319-30543338 MS.gene77612:intron 25.0%
!! TTTTCTTGAATCTGTTTTCA+GGG + chr2.1:30543130-30543149 MS.gene77612:intron 25.0%
!! TTTTTCTTGAATCTGTTTTC+AGG + chr2.1:30543129-30543148 MS.gene77612:intron 25.0%
!!! TTTCAATTTATTTTCTGTGC+AGG + chr2.1:30544890-30544909 MS.gene77612:intron 25.0%
!!! TTTTAGCTTTACACTTGATT+TGG - chr2.1:30544811-30544830 None:intergenic 25.0%
AATCTAAAAACCAGTCTACA+TGG - chr2.1:30544837-30544856 None:intergenic 30.0%
ACCATGTAAAATACACGATT+TGG + chr2.1:30544303-30544322 MS.gene77612:intron 30.0%
AGTCACTAATAACACACTTA+GGG - chr2.1:30543083-30543102 None:intergenic 30.0%
ATAGCAGTGTTATAGTTTAG+CGG + chr2.1:30543661-30543680 MS.gene77612:intron 30.0%
ATCTAAAAACCAGTCTACAT+GGG - chr2.1:30544836-30544855 None:intergenic 30.0%
ATTCCACTATAGCGATTTAA+GGG - chr2.1:30544364-30544383 None:intergenic 30.0%
ATTGAAAGAAGCTGAATTGA+AGG + chr2.1:30542486-30542505 MS.gene77612:CDS 30.0%
CCATGTAAAATACACGATTT+GGG + chr2.1:30544304-30544323 MS.gene77612:intron 30.0%
TAGTCACTAATAACACACTT+AGG - chr2.1:30543084-30543103 None:intergenic 30.0%
TATTAGTGACTATTGAGTGA+TGG + chr2.1:30543092-30543111 MS.gene77612:intron 30.0%
TTGAAAGAAGCTGAATTGAA+GGG + chr2.1:30542487-30542506 MS.gene77612:CDS 30.0%
TTGTAATGTTCAATTGTTCC+AGG + chr2.1:30543843-30543862 MS.gene77612:intron 30.0%
TTTCTGCAGGTTTATTTAAC+TGG - chr2.1:30544779-30544798 None:intergenic 30.0%
!! TAATACCTTGTTGTTAGCAT+CGG - chr2.1:30544169-30544188 None:intergenic 30.0%
AAAGTTGACATTCTCTTGCT+TGG + chr2.1:30543976-30543995 MS.gene77612:CDS 35.0%
AAGATTGCTGATGACTGTAT+TGG + chr2.1:30543344-30543363 MS.gene77612:CDS 35.0%
AATAGCAGCAAATGGTTTCT+TGG - chr2.1:30543902-30543921 None:intergenic 35.0%
AATGTCAACTTTCTCCAACA+AGG - chr2.1:30543968-30543987 None:intergenic 35.0%
ATAGCAGCAAATGGTTTCTT+GGG - chr2.1:30543901-30543920 None:intergenic 35.0%
ATCATATGGAATGGACCAAT+GGG + chr2.1:30544549-30544568 MS.gene77612:CDS 35.0%
ATTCACGTAAAGATCAGCAA+GGG - chr2.1:30543489-30543508 None:intergenic 35.0%
CAAAACCTTCAATGAAGCAT+TGG + chr2.1:30544512-30544531 MS.gene77612:CDS 35.0%
CATATACCAGATGACTAGAA+AGG - chr2.1:30542642-30542661 None:intergenic 35.0%
CATTCCACTATAGCGATTTA+AGG - chr2.1:30544365-30544384 None:intergenic 35.0%
GGTTGATCTAAATGTTCCTT+TGG + chr2.1:30542528-30542547 MS.gene77612:CDS 35.0%
TATGCAAAATTGCTTCATCG+TGG + chr2.1:30544690-30544709 MS.gene77612:intron 35.0%
TCTCAAAAAGCCAATCCAAA+CGG + chr2.1:30543056-30543075 MS.gene77612:intron 35.0%
TGATCTTGCAACTTCACTTA+TGG + chr2.1:30544074-30544093 MS.gene77612:CDS 35.0%
TTCACTTATGGAAAAAGCCA+AGG + chr2.1:30544086-30544105 MS.gene77612:CDS 35.0%
! AAACCATTTGCTGCTATTGT+TGG + chr2.1:30543904-30543923 MS.gene77612:CDS 35.0%
! ATCAAGTACTTGACTGGTTA+TGG + chr2.1:30542604-30542623 MS.gene77612:CDS 35.0%
! ATTATGCTTTTCGTGTGAGT+TGG + chr2.1:30544185-30544204 MS.gene77612:intron 35.0%
! CCCAAATCGTGTATTTTACA+TGG - chr2.1:30544307-30544326 None:intergenic 35.0%
! CTTTACGTGAATGATGCTTT+TGG + chr2.1:30543497-30543516 MS.gene77612:CDS 35.0%
! TATACCTTTTACAAGGCTCA+GGG + chr2.1:30544012-30544031 MS.gene77612:CDS 35.0%
! TGCATCTGTGAATGTGTTTT+CGG + chr2.1:30542673-30542692 MS.gene77612:intron 35.0%
! TTATACCTTTTACAAGGCTC+AGG + chr2.1:30544011-30544030 MS.gene77612:CDS 35.0%
! TTATGCTTTTCGTGTGAGTT+GGG + chr2.1:30544186-30544205 MS.gene77612:intron 35.0%
!! ACATTGCATGTCTTTAGAGT+TGG + chr2.1:30544717-30544736 MS.gene77612:intron 35.0%
!! TGCTTCATTGAAGGTTTTGA+TGG - chr2.1:30544511-30544530 None:intergenic 35.0%
!! TTGAAGGTTTTGATGGAATC+AGG - chr2.1:30544504-30544523 None:intergenic 35.0%
!! TTTTGCTTCTAGAGAATGTG+AGG + chr2.1:30543411-30543430 MS.gene77612:CDS 35.0%
AAGAAATTGGCAGAGGTAAG+TGG + chr2.1:30544921-30544940 MS.gene77612:CDS 40.0%
AAGATGAGTCACATCTCAAC+TGG + chr2.1:30545014-30545033 MS.gene77612:CDS 40.0%
AAGCTGAATTGAAGGGAAAG+AGG + chr2.1:30542494-30542513 MS.gene77612:CDS 40.0%
AAGGGAGTAACAACCATCAT+TGG + chr2.1:30544945-30544964 MS.gene77612:CDS 40.0%
ACAAGAGTCAGACCATCATA+TGG + chr2.1:30544535-30544554 MS.gene77612:CDS 40.0%
AGAGAGTCCTCAAAAAGGAA+AGG - chr2.1:30544231-30544250 None:intergenic 40.0%
AGAGGCTTCAAACTGTACTT+AGG - chr2.1:30543175-30543194 None:intergenic 40.0%
AGCTGAATTGAAGGGAAAGA+GGG + chr2.1:30542495-30542514 MS.gene77612:CDS 40.0%
AGTCAGACCATCATATGGAA+TGG + chr2.1:30544540-30544559 MS.gene77612:CDS 40.0%
CACATCAGTTGGAAGCAAAA+GGG - chr2.1:30544124-30544143 None:intergenic 40.0%
CATAGCTAAGAAATTGGCAG+AGG + chr2.1:30544914-30544933 MS.gene77612:CDS 40.0%
CATCATATGGAATGGACCAA+TGG + chr2.1:30544548-30544567 MS.gene77612:CDS 40.0%
CATTCACGTAAAGATCAGCA+AGG - chr2.1:30543490-30543509 None:intergenic 40.0%
CATTCTTCTCTTCTTCCTTG+TGG - chr2.1:30543439-30543458 None:intergenic 40.0%
CATTGGTCCATTCCATATGA+TGG - chr2.1:30544550-30544569 None:intergenic 40.0%
CCCATCCTCATTTGAACTTT+CGG - chr2.1:30544258-30544277 None:intergenic 40.0%
CCGAAAGTTCAAATGAGGAT+GGG + chr2.1:30544255-30544274 MS.gene77612:intron 40.0%
CCTACTATCAAGTACTTGAC+TGG + chr2.1:30542598-30542617 MS.gene77612:CDS 40.0%
CGAAAGTTCAAATGAGGATG+GGG + chr2.1:30544256-30544275 MS.gene77612:intron 40.0%
CGGAGTTATTGAATCCTTGT+TGG + chr2.1:30543951-30543970 MS.gene77612:CDS 40.0%
CTCAAAAAGCCAATCCAAAC+GGG + chr2.1:30543057-30543076 MS.gene77612:intron 40.0%
CTCTGCCAATTTCTTAGCTA+TGG - chr2.1:30544916-30544935 None:intergenic 40.0%
CTTGTCCAATGCTTCATTGA+AGG - chr2.1:30544520-30544539 None:intergenic 40.0%
GAAAATGCTAACGAACGAAG+AGG - chr2.1:30544639-30544658 None:intergenic 40.0%
GAGAGTCCTCAAAAAGGAAA+GGG - chr2.1:30544230-30544249 None:intergenic 40.0%
GCCAACTTCTTTGCAAATTC+AGG - chr2.1:30543464-30543483 None:intergenic 40.0%
GCCTGAATTTGCAAAGAAGT+TGG + chr2.1:30543460-30543479 MS.gene77612:CDS 40.0%
GCTAAAAAACCCATGTAGAC+TGG + chr2.1:30544824-30544843 MS.gene77612:intron 40.0%
GGATTCAATAACTCCGATCT+TGG - chr2.1:30543947-30543966 None:intergenic 40.0%
TAACGGCTATAGCAATGTTG+TGG + chr2.1:30543744-30543763 MS.gene77612:intron 40.0%
TAGCAGCAAATGGTTTCTTG+GGG - chr2.1:30543900-30543919 None:intergenic 40.0%
TCCGAAAGTTCAAATGAGGA+TGG + chr2.1:30544254-30544273 MS.gene77612:intron 40.0%
TTCGTCCGAAAGTTCAAATG+AGG + chr2.1:30544250-30544269 MS.gene77612:intron 40.0%
TTGCCCTTAAATCGCTATAG+TGG + chr2.1:30544358-30544377 MS.gene77612:intron 40.0%
TTGGAAAACACAGTTTCTGC+AGG - chr2.1:30544792-30544811 None:intergenic 40.0%
! ACAGTTCCCTTTCCTTTTTG+AGG + chr2.1:30544221-30544240 MS.gene77612:intron 40.0%
! AGTCATCCTTTCTAGTCATC+TGG + chr2.1:30542633-30542652 MS.gene77612:CDS 40.0%
! ATAACCCTGAGCCTTGTAAA+AGG - chr2.1:30544019-30544038 None:intergenic 40.0%
! CCAGTCAAGTACTTGATAGT+AGG - chr2.1:30542601-30542620 None:intergenic 40.0%
! CCTTATGCAGAAGGTATGTA+GGG + chr2.1:30543589-30543608 MS.gene77612:intron 40.0%
! GAAGTTGGTTGCACAAATTC+CGG + chr2.1:30543379-30543398 MS.gene77612:CDS 40.0%
! TACTTAGGTGTTACACCCTT+TGG - chr2.1:30543160-30543179 None:intergenic 40.0%
! TCCTTATGCAGAAGGTATGT+AGG + chr2.1:30543588-30543607 MS.gene77612:intron 40.0%
! TGTAGAAGCATGAGCTCTAT+GGG - chr2.1:30543528-30543547 None:intergenic 40.0%
!! GATGTAGTTTTGCTGAACTG+GGG + chr2.1:30544739-30544758 MS.gene77612:intron 40.0%
!! GGATGTAGTTTTGCTGAACT+GGG + chr2.1:30544738-30544757 MS.gene77612:intron 40.0%
!! TCAAAGGTGTCTTCCAAGAT+CGG + chr2.1:30543931-30543950 MS.gene77612:CDS 40.0%
!! TGGATGTAGTTTTGCTGAAC+TGG + chr2.1:30544737-30544756 MS.gene77612:intron 40.0%
AAAGAAACTAAGGGCCCGTT+TGG + chr2.1:30542950-30542969 MS.gene77612:intron 45.0%
AGAAGCAAAACACCACCTTC+CGG - chr2.1:30543401-30543420 None:intergenic 45.0%
AGAGAATGTGAGGTTCCACA+AGG + chr2.1:30543421-30543440 MS.gene77612:CDS 45.0%
AGAGCTCATGCTTCTACAGA+AGG + chr2.1:30543530-30543549 MS.gene77612:CDS 45.0%
ATAAGGAACCCTGCAACAGA+GGG - chr2.1:30543575-30543594 None:intergenic 45.0%
ATCATCAAGAGCAAGGACTC+CGG - chr2.1:30545081-30545100 None:intergenic 45.0%
ATGAGTCACATCTCAACTGG+TGG + chr2.1:30545017-30545036 MS.gene77612:CDS 45.0%
ATGGAAAAAGCCAAGGCGAA+AGG + chr2.1:30544093-30544112 MS.gene77612:CDS 45.0%
ATGTCGTCATTCGCAAATCG+CGG - chr2.1:30543787-30543806 None:intergenic 45.0%
CAACTAGATAGTCAAGCTCC+TGG - chr2.1:30543864-30543883 None:intergenic 45.0%
CAGGAGCTTGACTATCTAGT+TGG + chr2.1:30543862-30543881 MS.gene77612:intron 45.0%
CCAAGAAGTTCAGACAACCT+TGG - chr2.1:30543202-30543221 None:intergenic 45.0%
CCAAGGTTGTCTGAACTTCT+TGG + chr2.1:30543199-30543218 MS.gene77612:CDS 45.0%
CCACATCAGTTGGAAGCAAA+AGG - chr2.1:30544125-30544144 None:intergenic 45.0%
CCACCAACAATAGCAGCAAA+TGG - chr2.1:30543910-30543929 None:intergenic 45.0%
CCATCATCAAGTATCCCTGA+CGG + chr2.1:30544456-30544475 MS.gene77612:CDS 45.0%
CCCTACATACCTTCTGCATA+AGG - chr2.1:30543592-30543611 None:intergenic 45.0%
GACGAAGAGAGTCCTCAAAA+AGG - chr2.1:30544236-30544255 None:intergenic 45.0%
GCAGGCCATAGCTAAGAAAT+TGG + chr2.1:30544908-30544927 MS.gene77612:intron 45.0%
GGAGTAACAACCATCATTGG+TGG + chr2.1:30544948-30544967 MS.gene77612:CDS 45.0%
GGATGGATGGGATTGGATAT+TGG + chr2.1:30544477-30544496 MS.gene77612:CDS 45.0%
GTAACAACCATCATTGGTGG+TGG + chr2.1:30544951-30544970 MS.gene77612:CDS 45.0%
GTCTGAACTTCTTGGAACTC+AGG + chr2.1:30543207-30543226 MS.gene77612:CDS 45.0%
GTTGACATTCTCTTGCTTGG+TGG + chr2.1:30543979-30543998 MS.gene77612:CDS 45.0%
TCAGACAACCTTGGTACAAG+AGG - chr2.1:30543193-30543212 None:intergenic 45.0%
TCGAACTCAAAAACGCCCAT+TGG - chr2.1:30544567-30544586 None:intergenic 45.0%
TGAGTTCGACAAGTTTGCAG+CGG + chr2.1:30544578-30544597 MS.gene77612:CDS 45.0%
TGATAGTAGGAACAGCAGCA+CGG - chr2.1:30542588-30542607 None:intergenic 45.0%
TGGTGAGGAAGTTGAGAAGT+TGG + chr2.1:30543364-30543383 MS.gene77612:CDS 45.0%
TTGGTTGCACAAATTCCGGA+AGG + chr2.1:30543383-30543402 MS.gene77612:CDS 45.0%
! AGGGAAAGAGGGTTTTCGTT+AGG + chr2.1:30542506-30542525 MS.gene77612:CDS 45.0%
! ATGGCGACAAAAAGAAGCGT+GGG + chr2.1:30542460-30542479 MS.gene77612:CDS 45.0%
! CCATTTGCTGCTATTGTTGG+TGG + chr2.1:30543907-30543926 MS.gene77612:CDS 45.0%
! CCTTTTGCTTCCAACTGATG+TGG + chr2.1:30544122-30544141 MS.gene77612:CDS 45.0%
! CTGTAGAAGCATGAGCTCTA+TGG - chr2.1:30543529-30543548 None:intergenic 45.0%
! GGGAAAGAGGGTTTTCGTTA+GGG + chr2.1:30542507-30542526 MS.gene77612:CDS 45.0%
! GTTTGAAGCCTCTTGTACCA+AGG + chr2.1:30543182-30543201 MS.gene77612:CDS 45.0%
! TGCTGATGACTGTATTGGTG+AGG + chr2.1:30543349-30543368 MS.gene77612:CDS 45.0%
!! TGCTATTGTTGGTGGCTCAA+AGG + chr2.1:30543915-30543934 MS.gene77612:CDS 45.0%
!! TGTTTTCAGGGACGTCCAAA+GGG + chr2.1:30543142-30543161 MS.gene77612:intron 45.0%
!! TATAAAACATATAAATTTAA+AGG + chr2.1:30542830-30542849 MS.gene77612:intron 5.0%
AAGGCTCAGGGTTATGCCAT+TGG + chr2.1:30544024-30544043 MS.gene77612:CDS 50.0%
AAGGGAAACTCCTTTCGCCT+TGG - chr2.1:30544106-30544125 None:intergenic 50.0%
ACTTGAAGCCCTCTGTTGCA+GGG + chr2.1:30543564-30543583 MS.gene77612:CDS 50.0%
AGGCTCAGGGTTATGCCATT+GGG + chr2.1:30544025-30544044 MS.gene77612:CDS 50.0%
AGTCACATCTCAACTGGTGG+AGG + chr2.1:30545020-30545039 MS.gene77612:CDS 50.0%
ATTGGCAGAGGTAAGTGGCA+AGG + chr2.1:30544926-30544945 MS.gene77612:CDS 50.0%
CATAAGGAACCCTGCAACAG+AGG - chr2.1:30543576-30543595 None:intergenic 50.0%
CATCAAGTATCCCTGACGGA+TGG + chr2.1:30544460-30544479 MS.gene77612:CDS 50.0%
CCAGCTTAGAGCTTCTTGAG+GGG + chr2.1:30545045-30545064 MS.gene77612:CDS 50.0%
CCGTCAGGGATACTTGATGA+TGG - chr2.1:30544459-30544478 None:intergenic 50.0%
CTTGTCTTCCTCCACAAGAG+AGG - chr2.1:30544052-30544071 None:intergenic 50.0%
GAGTTCGACAAGTTTGCAGC+GGG + chr2.1:30544579-30544598 MS.gene77612:CDS 50.0%
GCCAGCTTAGAGCTTCTTGA+GGG + chr2.1:30545044-30545063 MS.gene77612:CDS 50.0%
GTTGCACAAATTCCGGAAGG+TGG + chr2.1:30543386-30543405 MS.gene77612:CDS 50.0%
TAACACACTTAGGGCCCGTT+TGG - chr2.1:30543074-30543093 None:intergenic 50.0%
TACTTGAAGCCCTCTGTTGC+AGG + chr2.1:30543563-30543582 MS.gene77612:CDS 50.0%
TCATCAAGAGCAAGGACTCC+GGG - chr2.1:30545080-30545099 None:intergenic 50.0%
TGCAGGGTTCCTTATGCAGA+AGG + chr2.1:30543580-30543599 MS.gene77612:CDS 50.0%
TGCCAGCTTAGAGCTTCTTG+AGG + chr2.1:30545043-30545062 MS.gene77612:CDS 50.0%
TGCTGCCGATGCTAACAACA+AGG + chr2.1:30544161-30544180 MS.gene77612:CDS 50.0%
TGGTTTCTTGGGGTTCGACA+CGG - chr2.1:30543890-30543909 None:intergenic 50.0%
TTGGCAGAGGTAAGTGGCAA+GGG + chr2.1:30544927-30544946 MS.gene77612:CDS 50.0%
! AAGTATCCCTGACGGATGGA+TGG + chr2.1:30544464-30544483 MS.gene77612:CDS 50.0%
! AGTATCCCTGACGGATGGAT+GGG + chr2.1:30544465-30544484 MS.gene77612:CDS 50.0%
! GACATTCTCTTGCTTGGTGG+AGG + chr2.1:30543982-30544001 MS.gene77612:CDS 50.0%
!! CTGTTTTCAGGGACGTCCAA+AGG + chr2.1:30543141-30543160 MS.gene77612:intron 50.0%
AGAGTCGCCACCACCAATGA+TGG - chr2.1:30544961-30544980 None:intergenic 55.0%
CAAGTTTGCAGCGGGAACAG+AGG + chr2.1:30544587-30544606 MS.gene77612:CDS 55.0%
CACTTAGGGCCCGTTTGGAT+TGG - chr2.1:30543069-30543088 None:intergenic 55.0%
CATCAAGAGCAAGGACTCCG+GGG - chr2.1:30545079-30545098 None:intergenic 55.0%
CATTGGGTCCTCTCTTGTGG+AGG + chr2.1:30544041-30544060 MS.gene77612:CDS 55.0%
CCAATCCCATCCATCCGTCA+GGG - chr2.1:30544473-30544492 None:intergenic 55.0%
CTCCACAAGAGAGGACCCAA+TGG - chr2.1:30544043-30544062 None:intergenic 55.0%
CTCTGTAGCAGCAGTCGAGA+AGG + chr2.1:30544977-30544996 MS.gene77612:CDS 55.0%
GTAGCAGCAGTCGAGAAGGT+TGG + chr2.1:30544981-30545000 MS.gene77612:CDS 55.0%
TCAGCGACGACCACATCAGT+TGG - chr2.1:30544135-30544154 None:intergenic 55.0%
TCCAATCCCATCCATCCGTC+AGG - chr2.1:30544474-30544493 None:intergenic 55.0%
TGCCATTGGGTCCTCTCTTG+TGG + chr2.1:30544038-30544057 MS.gene77612:CDS 55.0%
!! CCCCTCAAGAAGCTCTAAGC+TGG - chr2.1:30545048-30545067 None:intergenic 55.0%
! CCCTGACGGATGGATGGGAT+TGG + chr2.1:30544470-30544489 MS.gene77612:CDS 60.0%
AGAGCAAGGACTCCGGGGAG+TGG - chr2.1:30545074-30545093 None:intergenic 65.0%
CTTGAGGGGAAGCCACTCCC+CGG + chr2.1:30545059-30545078 MS.gene77612:CDS 65.0%


Chromosome Type Strat End Strand Name
chr2.1 gene 30542460 30545106 30542460 ID=MS.gene77612
chr2.1 mRNA 30542460 30545106 30542460 ID=MS.gene77612.t1;Parent=MS.gene77612
chr2.1 exon 30542460 30542654 30542460 ID=MS.gene77612.t1.exon1;Parent=MS.gene77612.t1
chr2.1 CDS 30542460 30542654 30542460 ID=cds.MS.gene77612.t1;Parent=MS.gene77612.t1
chr2.1 exon 30543151 30543228 30543151 ID=MS.gene77612.t1.exon2;Parent=MS.gene77612.t1
chr2.1 CDS 30543151 30543228 30543151 ID=cds.MS.gene77612.t1;Parent=MS.gene77612.t1
chr2.1 exon 30543341 30543601 30543341 ID=MS.gene77612.t1.exon3;Parent=MS.gene77612.t1
chr2.1 CDS 30543341 30543601 30543341 ID=cds.MS.gene77612.t1;Parent=MS.gene77612.t1
chr2.1 exon 30543865 30544182 30543865 ID=MS.gene77612.t1.exon4;Parent=MS.gene77612.t1
chr2.1 CDS 30543865 30544182 30543865 ID=cds.MS.gene77612.t1;Parent=MS.gene77612.t1
chr2.1 exon 30544450 30544608 30544450 ID=MS.gene77612.t1.exon5;Parent=MS.gene77612.t1
chr2.1 CDS 30544450 30544608 30544450 ID=cds.MS.gene77612.t1;Parent=MS.gene77612.t1
chr2.1 exon 30544912 30545106 30544912 ID=MS.gene77612.t1.exon6;Parent=MS.gene77612.t1
chr2.1 CDS 30544912 30545106 30544912 ID=cds.MS.gene77612.t1;Parent=MS.gene77612.t1
Gene Sequence

>MS.gene77612

ATGGCGACAAAAAGAAGCGTGGGAACATTGAAAGAAGCTGAATTGAAGGGAAAGAGGGTTTTCGTTAGGGTTGATCTAAATGTTCCTTTGGATGATAATTTGAATATCACTGATGATACTAGAATCCGTGCTGCTGTTCCTACTATCAAGTACTTGACTGGTTATGGTGCTAAAGTCATCCTTTCTAGTCATCTGGTATATGATCTATCTCTATGCATCTGTGAATGTGTTTTCGGTGTTTGAGTGTTTTTGATTTTTGATTATGATTCGAGTTAAATTGCTGCAACAATCTACTGAGATTTTATTAATGAGATAATGATATTTATGGTTTTTGTTTTGATTTAATTTAATTCTGTATTTGAGTGAGATCTATAAAACATATAAATTTAAAGGCTTATTAGTTTAATTGTTATGTGGTTGCTATTTTAGTTTAGTTTTTTTTTTTTACTTATTTGATTTTTCGAAGGTTTAGATGTACTAATGAAGAGATAAAGAAACTAAGGGCCCGTTTGGATTGACTTATTTTTGACATTATGAAAATTAGCTTATGAATAATATATAAAAGCTTATTTATTTGCATAAGCTGTCTTGCATAATCTCAAAAAGCCAATCCAAACGGGCCCTAAGTGTGTTATTAGTGACTATTGAGTGATGGTAGTAACTTGAAGTTTTTTCTTGAATCTGTTTTCAGGGACGTCCAAAGGGTGTAACACCTAAGTACAGTTTGAAGCCTCTTGTACCAAGGTTGTCTGAACTTCTTGGAACTCAGGCGTGTCTACTAAACTTTTCTGTTATAGTTTGACATTACGTTCAATCATTCTTATTTGCTTGTTTCCTTTGTGTAATAAGTTAATAATCCTGTTGATTATTCTTGGTTATAGGTTAAGATTGCTGATGACTGTATTGGTGAGGAAGTTGAGAAGTTGGTTGCACAAATTCCGGAAGGTGGTGTTTTGCTTCTAGAGAATGTGAGGTTCCACAAGGAAGAAGAGAAGAATGAGCCTGAATTTGCAAAGAAGTTGGCTTCCCTTGCTGATCTTTACGTGAATGATGCTTTTGGCACTGCCCATAGAGCTCATGCTTCTACAGAAGGTGTCGCTAAATACTTGAAGCCCTCTGTTGCAGGGTTCCTTATGCAGAAGGTATGTAGGGAGTAATTGTGATTTCGTTATATAATTGGCAGCATTGTTAAATAGCGACTATAGCAGTGTTATAGTTTAGCGGAATTAAATAAATATTGCTAATTGTTCTGGGATATGATATTTCGTACAAAATAATGTCTAATAACGGCTATAGCAATGTTGTGGTACGTAATTTGAACAGACCGCGATTTGCGAATGACGACATTGATATATTTGTTGATTAATGTAGTTGATTGATCTGTTGTAATGTTCAATTGTTCCAGGAGCTTGACTATCTAGTTGGAGCCGTGTCGAACCCCAAGAAACCATTTGCTGCTATTGTTGGTGGCTCAAAGGTGTCTTCCAAGATCGGAGTTATTGAATCCTTGTTGGAGAAAGTTGACATTCTCTTGCTTGGTGGAGGTATGATTTATACCTTTTACAAGGCTCAGGGTTATGCCATTGGGTCCTCTCTTGTGGAGGAAGACAAGCTTGATCTTGCAACTTCACTTATGGAAAAAGCCAAGGCGAAAGGAGTTTCCCTTTTGCTTCCAACTGATGTGGTCGTCGCTGATAAGTTTGCTGCCGATGCTAACAACAAGGTATTATGCTTTTCGTGTGAGTTGGGTTTAGCATTACAACAGTTCCCTTTCCTTTTTGAGGACTCTCTTCGTCCGAAAGTTCAAATGAGGATGGGGATACAATACGATCATGTGTTTGCAACCATGTAAAATACACGATTTGGGACTTTCATGTATGCTCTGCAATGTTGTCATATTGCCCTTAAATCGCTATAGTGGAATGTGAAGTAATCACTATTGTTTAGAATGTTGATATATATACTTAAAGCTTTGTTTATTTGTGCTCAGATTGTGCCATCATCAAGTATCCCTGACGGATGGATGGGATTGGATATTGGACCTGATTCCATCAAAACCTTCAATGAAGCATTGGACAAGAGTCAGACCATCATATGGAATGGACCAATGGGCGTTTTTGAGTTCGACAAGTTTGCAGCGGGAACAGAGGTATGTATGATTGATTTTGAAAGTGTTCCTCTTCGTTCGTTAGCATTTTCCATTAATAAATCATTCACCATTATTGAATGTTATGCAAAATTGCTTCATCGTGGCAGTACATTGCATGTCTTTAGAGTTGGATGTAGTTTTGCTGAACTGGGGAATATATGAATTGACCAGTTAAATAAACCTGCAGAAACTGTGTTTTCCAAATCAAGTGTAAAGCTAAAAAACCCATGTAGACTGGTTTTTAGATTCTTTTTTGAAGTTGAACAATCACACTCTTCTGTTTTCAATTTATTTTCTGTGCAGGCCATAGCTAAGAAATTGGCAGAGGTAAGTGGCAAGGGAGTAACAACCATCATTGGTGGTGGCGACTCTGTAGCAGCAGTCGAGAAGGTTGGACTAGCAGACAAGATGAGTCACATCTCAACTGGTGGAGGTGCCAGCTTAGAGCTTCTTGAGGGGAAGCCACTCCCCGGAGTCCTTGCTCTTGATGATGCTTAA
Protein sequence

>MS.gene77612.t1

MATKRSVGTLKEAELKGKRVFVRVDLNVPLDDNLNITDDTRIRAAVPTIKYLTGYGAKVILSSHLGRPKGVTPKYSLKPLVPRLSELLGTQVKIADDCIGEEVEKLVAQIPEGGVLLLENVRFHKEEEKNEPEFAKKLASLADLYVNDAFGTAHRAHASTEGVAKYLKPSVAGFLMQKELDYLVGAVSNPKKPFAAIVGGSKVSSKIGVIESLLEKVDILLLGGGMIYTFYKAQGYAIGSSLVEEDKLDLATSLMEKAKAKGVSLLLPTDVVVADKFAADANNKIVPSSSIPDGWMGLDIGPDSIKTFNEALDKSQTIIWNGPMGVFEFDKFAAGTEAIAKKLAEVSGKGVTTIIGGGDSVAAVEKVGLADKMSHISTGGGASLELLEGKPLPGVLALDDA