AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene78188

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene78188.t1 MTR_2g460810 97.546 489 12 0 1 489 1 489 0.0 947
MS.gene78188.t1 MTR_0004s0650 55.324 479 184 4 24 485 31 496 0.0 524
MS.gene78188.t1 MTR_4g099450 55.236 487 207 4 3 489 2 477 1.02e-174 501
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene78188.t1 AT2G13100 72.541 488 129 2 4 489 2 486 0.0 710
MS.gene78188.t1 AT2G13100 72.541 488 129 2 4 489 2 486 0.0 710
MS.gene78188.t1 AT3G47420 53.877 503 210 4 1 487 5 501 0.0 518
MS.gene78188.t1 AT3G47420 53.877 503 210 4 1 487 5 501 0.0 518
MS.gene78188.t1 AT3G47420 53.877 503 210 4 1 487 5 501 0.0 518
MS.gene78188.t1 AT1G30560 51.230 488 216 3 16 489 10 489 3.35e-177 507
MS.gene78188.t1 AT4G17550 51.044 527 216 4 1 486 1 526 1.50e-174 502
MS.gene78188.t1 AT4G25220 53.304 454 199 2 30 483 36 476 6.71e-172 494
MS.gene78188.t1 AT2G13100 73.092 249 64 1 241 489 89 334 6.37e-124 365
MS.gene78188.t1 AT2G13100 56.481 108 44 1 4 108 2 109 1.16e-31 124
MS.gene78188.t1 AT2G13100 77.982 218 48 0 272 489 83 300 3.17e-117 347
MS.gene78188.t1 AT2G13100 67.045 88 29 0 4 91 2 89 2.70e-32 125

Find 128 sgRNAs with CRISPR-Local

Find 167 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TTACTCCATTGGCATGTATT+TGG 0.217319 2.2:-33066842 MS.gene78188:CDS
ATCACCTTGATGTTTCTTTC+TGG 0.224719 2.2:-33065827 MS.gene78188:CDS
TTGAGGTTGTTTCTTGTGTT+TGG 0.258719 2.2:-33066793 MS.gene78188:CDS
CCTTGTGTTGTTGCAGTTGT+TGG 0.271567 2.2:-33066649 MS.gene78188:CDS
CAGTTGTTGGGAATTGGCTT+GGG 0.271735 2.2:-33066636 MS.gene78188:CDS
TAGTATTGTTAAAAGTGTTC+TGG 0.280648 2.2:-33066971 MS.gene78188:CDS
TGATAATAAGAACACATATT+TGG 0.281967 2.2:+33067037 None:intergenic
TTTGATATTGGAGGAGTTCT+AGG 0.290006 2.2:-33065980 MS.gene78188:CDS
CTCATTTCAATTTCCATTCC+AGG 0.291532 2.2:+33066412 None:intergenic
ATCAAAGAGGGGATTGATAA+TGG 0.292694 2.2:-33066611 MS.gene78188:CDS
AGTTATTGCTTCGGGTGTTT+TGG 0.295231 2.2:-33066545 MS.gene78188:CDS
TCAAATTCTTCGTCTGCTAT+TGG 0.299829 2.2:-33066310 MS.gene78188:CDS
TTGCTTACACTTTCTTGTAT+TGG 0.304564 2.2:-33066219 MS.gene78188:CDS
CTTTCAACAATATTTGATAT+TGG 0.314253 2.2:-33065992 MS.gene78188:CDS
TGTGGAGTGTTTCAGTCAAT+TGG 0.315612 2.2:-33066676 MS.gene78188:CDS
ACCTGTGTGCCTTATGTAGA+AGG 0.333717 2.2:+33066192 None:intergenic
TGAGGTTGTTTCTTGTGTTT+GGG 0.335721 2.2:-33066792 MS.gene78188:CDS
TGGTTTGATGTTCATGTGTT+GGG 0.336491 2.2:-33066724 MS.gene78188:CDS
GTGTTGGTTGCAGCGGTTGC+TGG 0.336822 2.2:-33066046 MS.gene78188:intron
CATTGGTTCAGTTATTGCTT+CGG 0.342494 2.2:-33066554 MS.gene78188:CDS
GCTTTAGTGGTGGGAATTCT+CGG 0.342732 2.2:-33065739 MS.gene78188:CDS
AACCAATTCAACCCTTGATT+CGG 0.343395 2.2:-33066935 MS.gene78188:CDS
GTTCCCCGAGTTCCATTAAA+AGG 0.343662 2.2:+33066904 None:intergenic
GGTTCTGTTGGAGCTGCTCT+TGG 0.349690 2.2:-33065680 MS.gene78188:CDS
CACATATTTGGTGGAATAGT+AGG 0.350389 2.2:+33067049 None:intergenic
GGGGATTGATAATGGGGATA+TGG 0.364452 2.2:-33066603 MS.gene78188:CDS
ACCAATTCAACCCTTGATTC+GGG 0.365317 2.2:-33066934 MS.gene78188:CDS
TCAAAGAGGGGATTGATAAT+GGG 0.369497 2.2:-33066610 MS.gene78188:CDS
GGTTGGTCGTTTGTGGTGCC+TGG 0.375096 2.2:-33066511 MS.gene78188:CDS
ACCCGAATCAAGGGTTGAAT+TGG 0.376330 2.2:+33066933 None:intergenic
AGCAGGTTATGTTTCAACTA+AGG 0.376999 2.2:-33065648 MS.gene78188:CDS
TTGTGAATCCCGTGGATATA+GGG 0.382740 2.2:-33066444 MS.gene78188:CDS
CTCGGTACGCAGGGCTTTAG+TGG 0.382958 2.2:-33065752 MS.gene78188:CDS
GCAGTTGTTGGGAATTGGCT+TGG 0.384000 2.2:-33066637 MS.gene78188:CDS
GTTTCTTGTGTTTGGGATGG+TGG 0.396297 2.2:-33066785 MS.gene78188:CDS
TTGTTGCAGTTGTTGGGAAT+TGG 0.403901 2.2:-33066642 MS.gene78188:CDS
TCCATTGGCATGTATTTGGC+TGG 0.414022 2.2:-33066838 MS.gene78188:CDS
TGGCTGGTCATGTTGGTGAT+AGG 0.414837 2.2:-33066822 MS.gene78188:CDS
GTTGTGAATCCCGTGGATAT+AGG 0.415165 2.2:-33066445 MS.gene78188:CDS
AGTATTGTTAAAAGTGTTCT+GGG 0.416808 2.2:-33066970 MS.gene78188:CDS
CTTGTGTTGTTGCAGTTGTT+GGG 0.419466 2.2:-33066648 MS.gene78188:CDS
GAATTTGAAATCGAACTATC+AGG 0.420709 2.2:+33066325 None:intergenic
ACGAAAATGATCAAAATTCC+AGG 0.423719 2.2:+33066493 None:intergenic
ACCTCGGTTGGGAATATCAT+TGG 0.423859 2.2:-33066571 MS.gene78188:CDS
ATTGGTTACCCTTCTACATA+AGG 0.433887 2.2:-33066201 MS.gene78188:CDS
ATTGGTTCAGTTATTGCTTC+GGG 0.436836 2.2:-33066553 MS.gene78188:CDS
CTGGTTTGATGTTCATGTGT+TGG 0.438300 2.2:-33066725 MS.gene78188:CDS
TGGATGGGGTTGGTCGTTTG+TGG 0.439831 2.2:-33066518 MS.gene78188:CDS
TTTCTTACTTCTTACTCCAT+TGG 0.444854 2.2:-33066853 MS.gene78188:CDS
ACTGTTACTGCAATCATTGA+TGG 0.445070 2.2:-33065707 MS.gene78188:CDS
AATCTATCTCACAAAACTGC+TGG 0.446010 2.2:-33066019 MS.gene78188:CDS
GTGGAGTGTTTCAGTCAATT+GGG 0.451035 2.2:-33066675 MS.gene78188:CDS
GTCGGTGTTCAGATTGTTTG+TGG 0.462172 2.2:-33066694 MS.gene78188:CDS
GATATAGGGTTTGCGAGTCC+TGG 0.467048 2.2:-33066430 MS.gene78188:CDS
TGGAATTCGCATACCTCGGT+TGG 0.474948 2.2:-33066583 MS.gene78188:CDS
ATGCTTCCTTTCATGCATCT+AGG 0.475318 2.2:-33067002 MS.gene78188:CDS
GGCTGCCGATCTCGGTACGC+AGG 0.479417 2.2:-33065762 MS.gene78188:CDS
TGGAGCTAGGCTCAAACTCT+TGG 0.480620 2.2:+33067113 None:intergenic
GCAGGTTATGTTTCAACTAA+GGG 0.481100 2.2:-33065647 MS.gene78188:CDS
CTTTAGTGGTGGGAATTCTC+GGG 0.483451 2.2:-33065738 MS.gene78188:CDS
ATTGGCTTGGGGAATCAAAG+AGG 0.486300 2.2:-33066624 MS.gene78188:CDS
ACTGCTGTGGCTGCCGATCT+CGG 0.489455 2.2:-33065770 MS.gene78188:CDS
TGGTGGGAATTCTCGGGCAC+TGG 0.495438 2.2:-33065732 MS.gene78188:CDS
AAGGCGGCCAACCCGAATCA+AGG 0.499130 2.2:+33066923 None:intergenic
TTTCTTGTGTTTGGGATGGT+GGG 0.499647 2.2:-33066784 MS.gene78188:CDS
AATCATATCAGAAATGAAAC+CGG 0.508019 2.2:+33065946 None:intergenic
TCAACAATATTTGATATTGG+AGG 0.508821 2.2:-33065989 MS.gene78188:CDS
GGTACGCAGGGCTTTAGTGG+TGG 0.512487 2.2:-33065749 MS.gene78188:CDS
ACTGCAATCATTGATGGTAC+TGG 0.512877 2.2:-33065701 MS.gene78188:CDS
AAAGCCCTGCGTACCGAGAT+CGG 0.516139 2.2:+33065757 None:intergenic
GTTGTTTCTTGTGTTTGGGA+TGG 0.516674 2.2:-33066788 MS.gene78188:CDS
GCACGATACAAAAGCAGAGC+TGG 0.518122 2.2:+33065878 None:intergenic
TATTCTTGATTCGAGTTGCA+AGG 0.520085 2.2:-33065574 MS.gene78188:CDS
GAATCAAGGGTTGAATTGGT+TGG 0.521050 2.2:+33066937 None:intergenic
ATTGAAATGAGTGTTGAAAC+GGG 0.522480 2.2:-33066400 MS.gene78188:CDS
GTGTTTGGGATGGTGGGCAG+CGG 0.524244 2.2:-33066778 MS.gene78188:CDS
ACATATTTGGTGGAATAGTA+GGG 0.524391 2.2:+33067050 None:intergenic
GAAACATAACCTGCTAAAAG+TGG 0.525372 2.2:+33065656 None:intergenic
GTTGAATTGGTTGGAACTGT+GGG 0.528850 2.2:+33066946 None:intergenic
GGAATTCGCATACCTCGGTT+GGG 0.531280 2.2:-33066582 MS.gene78188:CDS
GAATCGATAGAAACAAGAAC+AGG 0.531762 2.2:+33065900 None:intergenic
CAAAGAGGGGATTGATAATG+GGG 0.532878 2.2:-33066609 MS.gene78188:CDS
GTACGCAGGGCTTTAGTGGT+GGG 0.535609 2.2:-33065748 MS.gene78188:CDS
ACTCGGGGAACTCACCGGCT+TGG 0.537297 2.2:-33066892 MS.gene78188:CDS
TTAGAGGCGTGGAAAATACC+TGG 0.539044 2.2:-33066283 MS.gene78188:CDS
ATCTATCTCACAAAACTGCT+GGG 0.540237 2.2:-33066018 MS.gene78188:CDS
ATTCAACCCTTGATTCGGGT+TGG 0.543048 2.2:-33066930 MS.gene78188:CDS
AGTTGTTGGGAATTGGCTTG+GGG 0.544754 2.2:-33066635 MS.gene78188:CDS
AGAGATTCAGAGCTGGAGCT+AGG 0.548429 2.2:+33067100 None:intergenic
TAATAAGAACACATATTTGG+TGG 0.548927 2.2:+33067040 None:intergenic
GCTGCCGATCTCGGTACGCA+GGG 0.553404 2.2:-33065761 MS.gene78188:CDS
CGAGATCAACCTCGCCAAGC+CGG 0.554092 2.2:+33066878 None:intergenic
AAAGCAAACGGAGCGACTCC+AGG 0.556148 2.2:+33066265 None:intergenic
GGTTGAATTGGTTGGAACTG+TGG 0.556170 2.2:+33066945 None:intergenic
AGGGTTTGCGAGTCCTGGAA+TGG 0.556603 2.2:-33066425 MS.gene78188:CDS
AATTGAAATGAGTGTTGAAA+CGG 0.556957 2.2:-33066401 MS.gene78188:CDS
GGGAACTCACCGGCTTGGCG+AGG 0.557718 2.2:-33066887 MS.gene78188:CDS
ATGGAACTCGGGGAACTCAC+CGG 0.562728 2.2:-33066897 MS.gene78188:CDS
GCAGTTGTAATTAGCGAGTA+CGG 0.564151 2.2:+33065788 None:intergenic
ATTGATGGTACTGGTTCTGT+TGG 0.568619 2.2:-33065692 MS.gene78188:CDS
GTTATGTTTCAACTAAGGGA+TGG 0.570029 2.2:-33065643 MS.gene78188:CDS
AGGCTTCCTAGATGCATGAA+AGG 0.573970 2.2:+33066996 None:intergenic
GGTTTGATGTTCATGTGTTG+GGG 0.575219 2.2:-33066723 MS.gene78188:CDS
TTGGCTTGGGGAATCAAAGA+GGG 0.575740 2.2:-33066623 MS.gene78188:CDS
ATGTATTTGGCTGGTCATGT+TGG 0.577593 2.2:-33066829 MS.gene78188:CDS
TTTGGAAAGAGATTCAGAGC+TGG 0.577955 2.2:+33067093 None:intergenic
CCAACAACTGCAACAACACA+AGG 0.578535 2.2:+33066649 None:intergenic
AAATCCAGAAAGAAACATCA+AGG 0.579928 2.2:+33065823 None:intergenic
GTTCTAGGCGGAATCACCGC+CGG 0.584479 2.2:-33065965 MS.gene78188:CDS
TGTTGAAACGGGCGGTGCAG+AGG 0.585857 2.2:-33066389 MS.gene78188:CDS
CTCGCAAACCCTATATCCAC+GGG 0.599278 2.2:+33066436 None:intergenic
TCTGCAGAAAGTCGAATCAG+AGG 0.600067 2.2:-33066365 MS.gene78188:CDS
AGGCGGCCAACCCGAATCAA+GGG 0.601656 2.2:+33066924 None:intergenic
ACTCGCAAACCCTATATCCA+CGG 0.607916 2.2:+33066435 None:intergenic
ACCAGCCAAATACATGCCAA+TGG 0.609265 2.2:+33066837 None:intergenic
CTCGCTAATTACAACTGCTG+TGG 0.611850 2.2:-33065783 MS.gene78188:CDS
TTGGTGATAGGATTGATTTG+AGG 0.616292 2.2:-33066810 MS.gene78188:CDS
CCTGTGTGCCTTATGTAGAA+GGG 0.616762 2.2:+33066193 None:intergenic
CCCTTCTACATAAGGCACAC+AGG 0.627165 2.2:-33066193 MS.gene78188:intron
GATATGGAATTCGCATACCT+CGG 0.635544 2.2:-33066587 MS.gene78188:CDS
CCCCGAGTTCCATTAAAAGG+CGG 0.642431 2.2:+33066907 None:intergenic
GAGTGTTTCAGTCAATTGGG+TGG 0.653771 2.2:-33066672 MS.gene78188:CDS
GTTTCTTGTTGTGAATCCCG+TGG 0.657187 2.2:-33066452 MS.gene78188:CDS
TGGCTTGGGGAATCAAAGAG+GGG 0.677167 2.2:-33066622 MS.gene78188:CDS
ACCAATGATATTCCCAACCG+AGG 0.679777 2.2:+33066570 None:intergenic
CATATCAGAAATGAAACCGG+CGG 0.690344 2.2:+33065949 None:intergenic
GATATTGGAGGAGTTCTAGG+CGG 0.700532 2.2:-33065977 MS.gene78188:CDS
GAAATGAGTGTTGAAACGGG+CGG 0.861509 2.2:-33066397 MS.gene78188:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TATGTAAACTAAATAAGATA+TGG + chr2.2:33066558-33066577 None:intergenic 15.0%
!! CTTTCAACAATATTTGATAT+TGG - chr2.2:33066675-33066694 MS.gene78188:CDS 20.0%
!! TGATAATAAGAACACATATT+TGG + chr2.2:33065633-33065652 None:intergenic 20.0%
!!! GTTTTTTTACGATACTTTTT+GGG - chr2.2:33065911-33065930 MS.gene78188:CDS 20.0%
!!! TTGAATTTGTTATTTTGTGT+TGG - chr2.2:33066605-33066624 MS.gene78188:CDS 20.0%
! AAAAAGAGACAAAAAGCAAA+CGG + chr2.2:33066417-33066436 None:intergenic 25.0%
! AATCATATCAGAAATGAAAC+CGG + chr2.2:33066724-33066743 None:intergenic 25.0%
! TAATAAGAACACATATTTGG+TGG + chr2.2:33065630-33065649 None:intergenic 25.0%
! TCAACAATATTTGATATTGG+AGG - chr2.2:33066678-33066697 MS.gene78188:CDS 25.0%
!! AGAACACTTTTAACAATACT+AGG + chr2.2:33065697-33065716 None:intergenic 25.0%
!!! AATTGAAATGAGTGTTGAAA+CGG - chr2.2:33066266-33066285 MS.gene78188:CDS 25.0%
!!! AATTTTGATCATTTTCGTTG+GGG - chr2.2:33066179-33066198 MS.gene78188:intron 25.0%
!!! AGTATTGTTAAAAGTGTTCT+GGG - chr2.2:33065697-33065716 MS.gene78188:CDS 25.0%
!!! GAATTTTGATCATTTTCGTT+GGG - chr2.2:33066178-33066197 MS.gene78188:intron 25.0%
!!! GGTTTTTTTACGATACTTTT+TGG - chr2.2:33065910-33065929 MS.gene78188:CDS 25.0%
!!! TAGTATTGTTAAAAGTGTTC+TGG - chr2.2:33065696-33065715 MS.gene78188:CDS 25.0%
AAATCCAGAAAGAAACATCA+AGG + chr2.2:33066847-33066866 None:intergenic 30.0%
ACGAAAATGATCAAAATTCC+AGG + chr2.2:33066177-33066196 None:intergenic 30.0%
TTGCTTACACTTTCTTGTAT+TGG - chr2.2:33066448-33066467 MS.gene78188:CDS 30.0%
TTTCTTACTTCTTACTCCAT+TGG - chr2.2:33065814-33065833 MS.gene78188:CDS 30.0%
! ACACTTTTAACAATACTAGG+AGG + chr2.2:33065694-33065713 None:intergenic 30.0%
! ACATATTTGGTGGAATAGTA+GGG + chr2.2:33065620-33065639 None:intergenic 30.0%
! GAATTTGAAATCGAACTATC+AGG + chr2.2:33066345-33066364 None:intergenic 30.0%
! GATGTTTCTTTCTGGATTTT+TGG - chr2.2:33066848-33066867 MS.gene78188:CDS 30.0%
!! ATTGAAATGAGTGTTGAAAC+GGG - chr2.2:33066267-33066286 MS.gene78188:CDS 30.0%
!! GGAATTTTGATCATTTTCGT+TGG - chr2.2:33066177-33066196 MS.gene78188:intron 30.0%
!!! TTTACGATACTTTTTGGGTT+AGG - chr2.2:33065916-33065935 MS.gene78188:CDS 30.0%
AACCAATTCAACCCTTGATT+CGG - chr2.2:33065732-33065751 MS.gene78188:CDS 35.0%
AATCTATCTCACAAAACTGC+TGG - chr2.2:33066648-33066667 MS.gene78188:CDS 35.0%
ACTGTTACTGCAATCATTGA+TGG - chr2.2:33066960-33066979 MS.gene78188:CDS 35.0%
AGCAGGTTATGTTTCAACTA+AGG - chr2.2:33067019-33067038 MS.gene78188:CDS 35.0%
ATCAAAGAGGGGATTGATAA+TGG - chr2.2:33066056-33066075 MS.gene78188:intron 35.0%
ATCACCTTGATGTTTCTTTC+TGG - chr2.2:33066840-33066859 MS.gene78188:CDS 35.0%
ATCTATCTCACAAAACTGCT+GGG - chr2.2:33066649-33066668 MS.gene78188:CDS 35.0%
ATTGGTTACCCTTCTACATA+AGG - chr2.2:33066466-33066485 MS.gene78188:CDS 35.0%
CTCATTTCAATTTCCATTCC+AGG + chr2.2:33066258-33066277 None:intergenic 35.0%
GAAACATAACCTGCTAAAAG+TGG + chr2.2:33067014-33067033 None:intergenic 35.0%
GAATCGATAGAAACAAGAAC+AGG + chr2.2:33066770-33066789 None:intergenic 35.0%
GCAGGTTATGTTTCAACTAA+GGG - chr2.2:33067020-33067039 MS.gene78188:CDS 35.0%
GTTATGTTTCAACTAAGGGA+TGG - chr2.2:33067024-33067043 MS.gene78188:CDS 35.0%
TCAAATTCTTCGTCTGCTAT+TGG - chr2.2:33066357-33066376 MS.gene78188:CDS 35.0%
TGGTTTGATGTTCATGTGTT+GGG - chr2.2:33065943-33065962 MS.gene78188:CDS 35.0%
TTACTCCATTGGCATGTATT+TGG - chr2.2:33065825-33065844 MS.gene78188:CDS 35.0%
TTTGATATTGGAGGAGTTCT+AGG - chr2.2:33066687-33066706 MS.gene78188:CDS 35.0%
! ATTGGTTCAGTTATTGCTTC+GGG - chr2.2:33066114-33066133 MS.gene78188:intron 35.0%
! CACATATTTGGTGGAATAGT+AGG + chr2.2:33065621-33065640 None:intergenic 35.0%
! GAGATAAGAGAGAAGTTTTC+AGG - chr2.2:33067119-33067138 MS.gene78188:CDS 35.0%
! GTTTCGTCTATTTTCTTTGC+TGG - chr2.2:33067068-33067087 MS.gene78188:CDS 35.0%
! TCAAAGAGGGGATTGATAAT+GGG - chr2.2:33066057-33066076 MS.gene78188:intron 35.0%
!! CATTGGTTCAGTTATTGCTT+CGG - chr2.2:33066113-33066132 MS.gene78188:intron 35.0%
!! GTCTGCTATTGGATTTTTAG+AGG - chr2.2:33066368-33066387 MS.gene78188:CDS 35.0%
!! TATTCTTGATTCGAGTTGCA+AGG - chr2.2:33067093-33067112 MS.gene78188:CDS 35.0%
!! TGAGGTTGTTTCTTGTGTTT+GGG - chr2.2:33065875-33065894 MS.gene78188:CDS 35.0%
!! TTGAGGTTGTTTCTTGTGTT+TGG - chr2.2:33065874-33065893 MS.gene78188:CDS 35.0%
!! TTGGTGATAGGATTGATTTG+AGG - chr2.2:33065857-33065876 MS.gene78188:CDS 35.0%
!!! CTTTCTGGATTTTTGGTGAA+TGG - chr2.2:33066855-33066874 MS.gene78188:CDS 35.0%
!!! TACTTTTTGGGTTAGGATAC+TGG - chr2.2:33065923-33065942 MS.gene78188:CDS 35.0%
!!! TTATTTTGTGTTGGTTGCAG+CGG - chr2.2:33066614-33066633 MS.gene78188:CDS 35.0%
ACCAATTCAACCCTTGATTC+GGG - chr2.2:33065733-33065752 MS.gene78188:CDS 40.0%
ATGCTTCCTTTCATGCATCT+AGG - chr2.2:33065665-33065684 MS.gene78188:CDS 40.0%
CATATCAGAAATGAAACCGG+CGG + chr2.2:33066721-33066740 None:intergenic 40.0%
GAATCAAGGGTTGAATTGGT+TGG + chr2.2:33065733-33065752 None:intergenic 40.0%
GATATGGAATTCGCATACCT+CGG - chr2.2:33066080-33066099 MS.gene78188:intron 40.0%
GCAGTTGTAATTAGCGAGTA+CGG + chr2.2:33066882-33066901 None:intergenic 40.0%
GGTTTGATGTTCATGTGTTG+GGG - chr2.2:33065944-33065963 MS.gene78188:CDS 40.0%
GTTGAATTGGTTGGAACTGT+GGG + chr2.2:33065724-33065743 None:intergenic 40.0%
TTGTGAATCCCGTGGATATA+GGG - chr2.2:33066223-33066242 MS.gene78188:CDS 40.0%
TTGTTGCAGTTGTTGGGAAT+TGG - chr2.2:33066025-33066044 MS.gene78188:CDS 40.0%
TTTGGAAAGAGATTCAGAGC+TGG + chr2.2:33065577-33065596 None:intergenic 40.0%
! ACTGCAATCATTGATGGTAC+TGG - chr2.2:33066966-33066985 MS.gene78188:CDS 40.0%
! AGTTATTGCTTCGGGTGTTT+TGG - chr2.2:33066122-33066141 MS.gene78188:intron 40.0%
! ATGTATTTGGCTGGTCATGT+TGG - chr2.2:33065838-33065857 MS.gene78188:CDS 40.0%
! CTGGTTTGATGTTCATGTGT+TGG - chr2.2:33065942-33065961 MS.gene78188:CDS 40.0%
! CTTGTGTTGTTGCAGTTGTT+GGG - chr2.2:33066019-33066038 MS.gene78188:CDS 40.0%
! GATCATTTTCGTTGGGGTTT+TGG - chr2.2:33066185-33066204 MS.gene78188:intron 40.0%
! GTGGAGTGTTTCAGTCAATT+GGG - chr2.2:33065992-33066011 MS.gene78188:CDS 40.0%
! TGCTTTTGTATCGTGCTTTC+GGG - chr2.2:33066796-33066815 MS.gene78188:CDS 40.0%
! TGTGGAGTGTTTCAGTCAAT+TGG - chr2.2:33065991-33066010 MS.gene78188:CDS 40.0%
! TTTCTTGTGTTTGGGATGGT+GGG - chr2.2:33065883-33065902 MS.gene78188:CDS 40.0%
!! AATAGTAGGGTTTTGTGAGG+GGG + chr2.2:33065607-33065626 None:intergenic 40.0%
!! ATTGATGGTACTGGTTCTGT+TGG - chr2.2:33066975-33066994 MS.gene78188:CDS 40.0%
!! CAAAGAGGGGATTGATAATG+GGG - chr2.2:33066058-33066077 MS.gene78188:intron 40.0%
!! CTATTGGATTTTTAGAGGCG+TGG - chr2.2:33066373-33066392 MS.gene78188:CDS 40.0%
!! CTGCTTTTGTATCGTGCTTT+CGG - chr2.2:33066795-33066814 MS.gene78188:CDS 40.0%
!! GAATAGTAGGGTTTTGTGAG+GGG + chr2.2:33065608-33065627 None:intergenic 40.0%
!! GGAATAGTAGGGTTTTGTGA+GGG + chr2.2:33065609-33065628 None:intergenic 40.0%
!! GTTGTTTCTTGTGTTTGGGA+TGG - chr2.2:33065879-33065898 MS.gene78188:CDS 40.0%
!! TGGAATAGTAGGGTTTTGTG+AGG + chr2.2:33065610-33065629 None:intergenic 40.0%
!!! CATGTGTTGGGGTTTTTTGT+CGG - chr2.2:33065955-33065974 MS.gene78188:CDS 40.0%
!!! GGGTGTTTTGGAATTTGGAT+GGG - chr2.2:33066134-33066153 MS.gene78188:intron 40.0%
!!! GGTGTTTTGGAATTTGGATG+GGG - chr2.2:33066135-33066154 MS.gene78188:intron 40.0%
!!! TTTTGGAATTTGGATGGGGT+TGG - chr2.2:33066139-33066158 MS.gene78188:intron 40.0%
ACCAATGATATTCCCAACCG+AGG + chr2.2:33066100-33066119 None:intergenic 45.0%
ACCAGCCAAATACATGCCAA+TGG + chr2.2:33065833-33065852 None:intergenic 45.0%
ACCCGAATCAAGGGTTGAAT+TGG + chr2.2:33065737-33065756 None:intergenic 45.0%
ACCTCGGTTGGGAATATCAT+TGG - chr2.2:33066096-33066115 MS.gene78188:intron 45.0%
ACCTGTGTGCCTTATGTAGA+AGG + chr2.2:33066478-33066497 None:intergenic 45.0%
ACTCGCAAACCCTATATCCA+CGG + chr2.2:33066235-33066254 None:intergenic 45.0%
AGGCTTCCTAGATGCATGAA+AGG + chr2.2:33065674-33065693 None:intergenic 45.0%
CCAACAACTGCAACAACACA+AGG + chr2.2:33066021-33066040 None:intergenic 45.0%
CCTGTGTGCCTTATGTAGAA+GGG + chr2.2:33066477-33066496 None:intergenic 45.0%
CTCGCTAATTACAACTGCTG+TGG - chr2.2:33066884-33066903 MS.gene78188:CDS 45.0%
GAGTGTTTCAGTCAATTGGG+TGG - chr2.2:33065995-33066014 MS.gene78188:CDS 45.0%
GGTTGAATTGGTTGGAACTG+TGG + chr2.2:33065725-33065744 None:intergenic 45.0%
GTTCCCCGAGTTCCATTAAA+AGG + chr2.2:33065766-33065785 None:intergenic 45.0%
GTTGTGAATCCCGTGGATAT+AGG - chr2.2:33066222-33066241 MS.gene78188:CDS 45.0%
TCCATTGGCATGTATTTGGC+TGG - chr2.2:33065829-33065848 MS.gene78188:CDS 45.0%
TCTGCAGAAAGTCGAATCAG+AGG - chr2.2:33066302-33066321 MS.gene78188:CDS 45.0%
! CAGTTGTTGGGAATTGGCTT+GGG - chr2.2:33066031-33066050 MS.gene78188:CDS 45.0%
! CCTTGTGTTGTTGCAGTTGT+TGG - chr2.2:33066018-33066037 MS.gene78188:CDS 45.0%
! CTTTAGTGGTGGGAATTCTC+GGG - chr2.2:33066929-33066948 MS.gene78188:CDS 45.0%
! GCTTTAGTGGTGGGAATTCT+CGG - chr2.2:33066928-33066947 MS.gene78188:CDS 45.0%
! GTCGGTGTTCAGATTGTTTG+TGG - chr2.2:33065973-33065992 MS.gene78188:CDS 45.0%
! GTTTCTTGTGTTTGGGATGG+TGG - chr2.2:33065882-33065901 MS.gene78188:CDS 45.0%
! GTTTCTTGTTGTGAATCCCG+TGG - chr2.2:33066215-33066234 MS.gene78188:CDS 45.0%
! TTAGAGGCGTGGAAAATACC+TGG - chr2.2:33066384-33066403 MS.gene78188:CDS 45.0%
!! AGTTGTTGGGAATTGGCTTG+GGG - chr2.2:33066032-33066051 MS.gene78188:CDS 45.0%
!! ATTCAACCCTTGATTCGGGT+TGG - chr2.2:33065737-33065756 MS.gene78188:CDS 45.0%
!! ATTGGCTTGGGGAATCAAAG+AGG - chr2.2:33066043-33066062 MS.gene78188:intron 45.0%
!! GAAATGAGTGTTGAAACGGG+CGG - chr2.2:33066270-33066289 MS.gene78188:CDS 45.0%
!! GATATTGGAGGAGTTCTAGG+CGG - chr2.2:33066690-33066709 MS.gene78188:CDS 45.0%
!! GGGGATTGATAATGGGGATA+TGG - chr2.2:33066064-33066083 MS.gene78188:intron 45.0%
!! TTGGCTTGGGGAATCAAAGA+GGG - chr2.2:33066044-33066063 MS.gene78188:intron 45.0%
!!! CGGGTGTTTTGGAATTTGGA+TGG - chr2.2:33066133-33066152 MS.gene78188:intron 45.0%
!!! GCTTCGGGTGTTTTGGAATT+TGG - chr2.2:33066129-33066148 MS.gene78188:intron 45.0%
AGAGATTCAGAGCTGGAGCT+AGG + chr2.2:33065570-33065589 None:intergenic 50.0%
CCCCGAGTTCCATTAAAAGG+CGG + chr2.2:33065763-33065782 None:intergenic 50.0%
CCCTTCTACATAAGGCACAC+AGG - chr2.2:33066474-33066493 MS.gene78188:CDS 50.0%
CTCGCAAACCCTATATCCAC+GGG + chr2.2:33066234-33066253 None:intergenic 50.0%
GATATAGGGTTTGCGAGTCC+TGG - chr2.2:33066237-33066256 MS.gene78188:CDS 50.0%
GCACGATACAAAAGCAGAGC+TGG + chr2.2:33066792-33066811 None:intergenic 50.0%
GCAGTTGTTGGGAATTGGCT+TGG - chr2.2:33066030-33066049 MS.gene78188:CDS 50.0%
GGAATTCGCATACCTCGGTT+GGG - chr2.2:33066085-33066104 MS.gene78188:intron 50.0%
TGGAATTCGCATACCTCGGT+TGG - chr2.2:33066084-33066103 MS.gene78188:intron 50.0%
TGGAGCTAGGCTCAAACTCT+TGG + chr2.2:33065557-33065576 None:intergenic 50.0%
! CCGCCTTTTAATGGAACTCG+GGG - chr2.2:33065760-33065779 MS.gene78188:CDS 50.0%
! GCCGCCTTTTAATGGAACTC+GGG - chr2.2:33065759-33065778 MS.gene78188:CDS 50.0%
! GGCCGCCTTTTAATGGAACT+CGG - chr2.2:33065758-33065777 MS.gene78188:CDS 50.0%
! TGGCTTGGGGAATCAAAGAG+GGG - chr2.2:33066045-33066064 MS.gene78188:intron 50.0%
!! GCTCTTGGACCACTTTTAGC+AGG - chr2.2:33067002-33067021 MS.gene78188:CDS 50.0%
!! TGGCTGGTCATGTTGGTGAT+AGG - chr2.2:33065845-33065864 MS.gene78188:CDS 50.0%
!! TTGTGAGGGGGTTTCAGGTT+TGG + chr2.2:33065595-33065614 None:intergenic 50.0%
AAAGCAAACGGAGCGACTCC+AGG + chr2.2:33066405-33066424 None:intergenic 55.0%
AAAGCCCTGCGTACCGAGAT+CGG + chr2.2:33066913-33066932 None:intergenic 55.0%
AGGGTTTGCGAGTCCTGGAA+TGG - chr2.2:33066242-33066261 MS.gene78188:CDS 55.0%
ATGGAACTCGGGGAACTCAC+CGG - chr2.2:33065770-33065789 MS.gene78188:CDS 55.0%
GGTTCTGTTGGAGCTGCTCT+TGG - chr2.2:33066987-33067006 MS.gene78188:CDS 55.0%
! GTACGCAGGGCTTTAGTGGT+GGG - chr2.2:33066919-33066938 MS.gene78188:CDS 55.0%
!! GGGTTTTGTGAGGGGGTTTC+AGG + chr2.2:33065600-33065619 None:intergenic 55.0%
!! TGGATGGGGTTGGTCGTTTG+TGG - chr2.2:33066149-33066168 MS.gene78188:intron 55.0%
!!! TCGGGTTGGCCGCCTTTTAA+TGG - chr2.2:33065751-33065770 MS.gene78188:CDS 55.0%
AAGGCGGCCAACCCGAATCA+AGG + chr2.2:33065747-33065766 None:intergenic 60.0%
ACTGCTGTGGCTGCCGATCT+CGG - chr2.2:33066897-33066916 MS.gene78188:CDS 60.0%
AGGCGGCCAACCCGAATCAA+GGG + chr2.2:33065746-33065765 None:intergenic 60.0%
CGAGATCAACCTCGCCAAGC+CGG + chr2.2:33065792-33065811 None:intergenic 60.0%
TGGTGGGAATTCTCGGGCAC+TGG - chr2.2:33066935-33066954 MS.gene78188:CDS 60.0%
TGTTGAAACGGGCGGTGCAG+AGG - chr2.2:33066278-33066297 MS.gene78188:CDS 60.0%
! CTCGGTACGCAGGGCTTTAG+TGG - chr2.2:33066915-33066934 MS.gene78188:CDS 60.0%
! GGTACGCAGGGCTTTAGTGG+TGG - chr2.2:33066918-33066937 MS.gene78188:CDS 60.0%
! GGTTGGTCGTTTGTGGTGCC+TGG - chr2.2:33066156-33066175 MS.gene78188:intron 60.0%
! GTTCTAGGCGGAATCACCGC+CGG - chr2.2:33066702-33066721 MS.gene78188:CDS 60.0%
!! GTGTTGGTTGCAGCGGTTGC+TGG - chr2.2:33066621-33066640 MS.gene78188:CDS 60.0%
!! GTGTTTGGGATGGTGGGCAG+CGG - chr2.2:33065889-33065908 MS.gene78188:CDS 60.0%
GCTGCCGATCTCGGTACGCA+GGG - chr2.2:33066906-33066925 MS.gene78188:CDS 65.0%
! ACTCGGGGAACTCACCGGCT+TGG - chr2.2:33065775-33065794 MS.gene78188:CDS 65.0%
GGCTGCCGATCTCGGTACGC+AGG - chr2.2:33066905-33066924 MS.gene78188:CDS 70.0%
!! GGGAACTCACCGGCTTGGCG+AGG - chr2.2:33065780-33065799 MS.gene78188:CDS 70.0%


Chromosome Type Strat End Strand Name
chr2.2 gene 33065541 33067148 33065541 ID=MS.gene78188
chr2.2 mRNA 33065541 33067148 33065541 ID=MS.gene78188.t1;Parent=MS.gene78188
chr2.2 exon 33066194 33067148 33066194 ID=MS.gene78188.t1.exon1;Parent=MS.gene78188.t1
chr2.2 CDS 33066194 33067148 33066194 ID=cds.MS.gene78188.t1;Parent=MS.gene78188.t1
chr2.2 exon 33065541 33066055 33065541 ID=MS.gene78188.t1.exon2;Parent=MS.gene78188.t1
chr2.2 CDS 33065541 33066055 33065541 ID=cds.MS.gene78188.t1;Parent=MS.gene78188.t1
Gene Sequence

>MS.gene78188

ATGGAATCAAATTCCAAGAGTTTGAGCCTAGCTCCAGCTCTGAATCTCTTTCCAAACCTGAAACCCCCTCACAAAACCCTACTATTCCACCAAATATGTGTTCTTATTATCACATTTCTTGCATATGCTTCCTTTCATGCATCTAGGAAGCCTCCTAGTATTGTTAAAAGTGTTCTGGGACCCACAGTTCCAACCAATTCAACCCTTGATTCGGGTTGGCCGCCTTTTAATGGAACTCGGGGAACTCACCGGCTTGGCGAGGTTGATCTCGCATTTCTTACTTCTTACTCCATTGGCATGTATTTGGCTGGTCATGTTGGTGATAGGATTGATTTGAGGTTGTTTCTTGTGTTTGGGATGGTGGGCAGCGGTTTTTTTACGATACTTTTTGGGTTAGGATACTGGTTTGATGTTCATGTGTTGGGGTTTTTTGTCGGTGTTCAGATTGTTTGTGGAGTGTTTCAGTCAATTGGGTGGCCTTGTGTTGTTGCAGTTGTTGGGAATTGGCTTGGGGAATCAAAGAGGGGATTGATAATGGGGATATGGAATTCGCATACCTCGGTTGGGAATATCATTGGTTCAGTTATTGCTTCGGGTGTTTTGGAATTTGGATGGGGTTGGTCGTTTGTGGTGCCTGGAATTTTGATCATTTTCGTTGGGGTTTTGGTGTTTCTGTTTCTTGTTGTGAATCCCGTGGATATAGGGTTTGCGAGTCCTGGAATGGAAATTGAAATGAGTGTTGAAACGGGCGGTGCAGAGGATCTGCAGAAAGTCGAATCAGAGGAAGCAAAGCTAATTGATCCTGATAGTTCGATTTCAAATTCTTCGTCTGCTATTGGATTTTTAGAGGCGTGGAAAATACCTGGAGTCGCTCCGTTTGCTTTTTGTCTCTTTTTCTCAAAATTTGTTGCTTACACTTTCTTGTATTGGTTACCCTTCTACATAAGGCACACAGGTAATTGTTCATTGATGCAAAAATATATCATATCTGATAGATTTATTGCATTGATTAAGCCATATCTTATTTAGTTTACATAATTTTCATGAATTATGATTTTTTTTTATTGAATTTGTTATTTTGTGTTGGTTGCAGCGGTTGCTGGTGTGAATCTATCTCACAAAACTGCTGGGTTACTTTCAACAATATTTGATATTGGAGGAGTTCTAGGCGGAATCACCGCCGGTTTCATTTCTGATATGATTGAAGCTCGCGCTGTGACTTCAATCCTGTTCTTGTTTCTATCGATTCCAGCTCTGCTTTTGTATCGTGCTTTCGGGAGCATCTCTATGTTTACTAACATCACCTTGATGTTTCTTTCTGGATTTTTGGTGAATGGTCCGTACTCGCTAATTACAACTGCTGTGGCTGCCGATCTCGGTACGCAGGGCTTTAGTGGTGGGAATTCTCGGGCACTGGCTACTGTTACTGCAATCATTGATGGTACTGGTTCTGTTGGAGCTGCTCTTGGACCACTTTTAGCAGGTTATGTTTCAACTAAGGGATGGAACAGTGTATTTTTTATGCTCGTTTCGTCTATTTTCTTTGCTGGTTTATTCTTGATTCGAGTTGCAAGGACTGAGATAAGAGAGAAGTTTTCAGGAAAGTGA
Protein sequence

>MS.gene78188.t1

MESNSKSLSLAPALNLFPNLKPPHKTLLFHQICVLIITFLAYASFHASRKPPSIVKSVLGPTVPTNSTLDSGWPPFNGTRGTHRLGEVDLAFLTSYSIGMYLAGHVGDRIDLRLFLVFGMVGSGFFTILFGLGYWFDVHVLGFFVGVQIVCGVFQSIGWPCVVAVVGNWLGESKRGLIMGIWNSHTSVGNIIGSVIASGVLEFGWGWSFVVPGILIIFVGVLVFLFLVVNPVDIGFASPGMEIEMSVETGGAEDLQKVESEEAKLIDPDSSISNSSSAIGFLEAWKIPGVAPFAFCLFFSKFVAYTFLYWLPFYIRHTAVAGVNLSHKTAGLLSTIFDIGGVLGGITAGFISDMIEARAVTSILFLFLSIPALLLYRAFGSISMFTNITLMFLSGFLVNGPYSLITTAVAADLGTQGFSGGNSRALATVTAIIDGTGSVGAALGPLLAGYVSTKGWNSVFFMLVSSIFFAGLFLIRVARTEIREKFSGK