Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene78345.t1 | XP_003626318.2 | 86.8 | 333 | 13 | 5 | 1 | 324 | 6 | 316 | 1.40E-145 | 525.8 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene78345.t1 | Q9SK91 | 44.3 | 325 | 145 | 8 | 9 | 318 | 1 | 304 | 1.7e-54 | 214.5 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene78345.t1 | G7KZP3 | 86.8 | 333 | 13 | 5 | 1 | 324 | 6 | 316 | 1.0e-145 | 525.8 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene049142 | MS.gene78345 | 0.803874 | 2.83E-49 | -1.69E-46 |
| MS.gene049350 | MS.gene78345 | 0.815985 | 6.98E-52 | -1.69E-46 |
| MS.gene049711 | MS.gene78345 | 0.820117 | 8.13E-53 | -1.69E-46 |
| MS.gene051720 | MS.gene78345 | 0.800241 | 1.58E-48 | -1.69E-46 |
| MS.gene052463 | MS.gene78345 | 0.806273 | 8.91E-50 | -1.69E-46 |
| MS.gene054991 | MS.gene78345 | 0.803099 | 4.10E-49 | -1.69E-46 |
| MS.gene057102 | MS.gene78345 | 0.800416 | 1.46E-48 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 43 sgRNAs with CRISPR-Local
Find 71 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| AGAATTATGCTTATGGAAAT+TGG | 0.223168 | 7.1:-2691395 | MS.gene78345:CDS |
| ACCATCTTCAAGAGCTGTTT+TGG | 0.261447 | 7.1:+2690670 | None:intergenic |
| GGTGATCAAGCATCAATTAT+TGG | 0.265730 | 7.1:-2690946 | MS.gene78345:CDS |
| GTTGAATTCTTTCCAACATT+TGG | 0.301786 | 7.1:+2690344 | None:intergenic |
| GAGCAGAAGCTACAATTTCT+TGG | 0.311027 | 7.1:-2690895 | MS.gene78345:CDS |
| GTGATCAAGCATCAATTATT+GGG | 0.314095 | 7.1:-2690945 | MS.gene78345:CDS |
| CATCAGCTGTAGTGACATTA+AGG | 0.320979 | 7.1:+2690611 | None:intergenic |
| ACCCTTTGGACGTAAGATTC+AGG | 0.337296 | 7.1:+2691132 | None:intergenic |
| TTGCTTCAGAATCTGTTAAC+AGG | 0.346873 | 7.1:+2691493 | None:intergenic |
| CAAACAGAGAATTATGCTTA+TGG | 0.362320 | 7.1:-2691402 | MS.gene78345:CDS |
| CTTCTATTCTATCAGCGTTA+AGG | 0.373348 | 7.1:-2690581 | MS.gene78345:intron |
| AAGCATAATTCTCTGTTTGT+TGG | 0.375728 | 7.1:+2691406 | None:intergenic |
| GTTGAAGATGATTGCAAGCT+TGG | 0.385709 | 7.1:-2690397 | MS.gene78345:CDS |
| ACAATCATTGTTGCCACAAA+TGG | 0.405629 | 7.1:-2691337 | MS.gene78345:CDS |
| CCACAAGACCCTTTCACCTA+TGG | 0.431613 | 7.1:-2691543 | MS.gene78345:CDS |
| TTAACAGGTTGTAATTGTAA+TGG | 0.451750 | 7.1:+2691508 | None:intergenic |
| ACCAAAACAGCTCTTGAAGA+TGG | 0.460812 | 7.1:-2690671 | MS.gene78345:CDS |
| ACATTGCTGTAGAACGCAAC+AGG | 0.469202 | 7.1:-2691200 | MS.gene78345:CDS |
| TTCTTACTTTGAGATTTGCA+TGG | 0.473698 | 7.1:+2690704 | None:intergenic |
| GAAATTGAACTATCTAAAGA+TGG | 0.481389 | 7.1:+2691300 | None:intergenic |
| TGTTGAATCCATAGGTGAAA+GGG | 0.493303 | 7.1:+2691535 | None:intergenic |
| AAGATGGTTTCTAGTTTGCA+TGG | 0.504712 | 7.1:-2690655 | MS.gene78345:CDS |
| GGCAACAATGATTGTGGTGG+TGG | 0.505665 | 7.1:+2691345 | None:intergenic |
| TTTGACACTTCATCCATTTG+TGG | 0.511454 | 7.1:+2691324 | None:intergenic |
| TGCCTGAATCTTACGTCCAA+AGG | 0.512531 | 7.1:-2691134 | MS.gene78345:CDS |
| GCCTGAATCTTACGTCCAAA+GGG | 0.523230 | 7.1:-2691133 | MS.gene78345:intron |
| TCTTGTGGATATAGTAGAGT+TGG | 0.525531 | 7.1:+2691558 | None:intergenic |
| AACAATGATTGTGGTGGTGG+TGG | 0.531255 | 7.1:+2691348 | None:intergenic |
| CTGTCACTTGTTGAGTATTG+TGG | 0.536473 | 7.1:+2690811 | None:intergenic |
| TTGTTGAATCCATAGGTGAA+AGG | 0.539793 | 7.1:+2691534 | None:intergenic |
| TAGAGTTGGTTCTAGTGCCA+TGG | 0.564316 | 7.1:+2691572 | None:intergenic |
| CCATAGGTGAAAGGGTCTTG+TGG | 0.567251 | 7.1:+2691543 | None:intergenic |
| ATGGTCTTTGTTGAATCCAT+AGG | 0.572517 | 7.1:+2691527 | None:intergenic |
| TGCAGCTGTATACCAAATGT+TGG | 0.583918 | 7.1:-2690356 | MS.gene78345:CDS |
| TGATTGCAAGCTTGGATCAG+TGG | 0.587540 | 7.1:-2690389 | MS.gene78345:CDS |
| CAGAAGCTACAATTTCTTGG+TGG | 0.593039 | 7.1:-2690892 | MS.gene78345:CDS |
| TGATCAAGCATCAATTATTG+GGG | 0.599410 | 7.1:-2690944 | MS.gene78345:CDS |
| TAGAGAAAGAGAGAGATCCA+TGG | 0.615999 | 7.1:-2691589 | MS.gene78345:CDS |
| TGCTGATATTGAAGTGACAA+TGG | 0.646131 | 7.1:-2690734 | MS.gene78345:CDS |
| ATTTGTGGCAACAATGATTG+TGG | 0.649128 | 7.1:+2691339 | None:intergenic |
| ACTTGTTGAGTATTGTGGAA+AGG | 0.653314 | 7.1:+2690816 | None:intergenic |
| TGTGGCAACAATGATTGTGG+TGG | 0.680500 | 7.1:+2691342 | None:intergenic |
| GATCAAGCATCAATTATTGG+GGG | 0.757063 | 7.1:-2690943 | MS.gene78345:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !!! | AATTTTATTTTGTAATTTGT+AGG | - | chr7.1:2691492-2691511 | MS.gene78345:CDS | 10.0% |
| !! | CAATCAATATATTGAATTAA+AGG | - | chr7.1:2691599-2691618 | MS.gene78345:CDS | 15.0% |
| !!! | TCATTTTTAAATTGAAATTG+CGG | + | chr7.1:2690917-2690936 | None:intergenic | 15.0% |
| !! | AAGAAAAAAAAAACATGACA+GGG | + | chr7.1:2691370-2691389 | None:intergenic | 20.0% |
| !! | TTAAGTGCTTACAATAATTT+AGG | + | chr7.1:2691445-2691464 | None:intergenic | 20.0% |
| !!! | CTTCTATTACTTTTAAATTG+TGG | - | chr7.1:2690803-2690822 | MS.gene78345:CDS | 20.0% |
| !!! | TTTGATTGATGAATTTTTCA+GGG | - | chr7.1:2690944-2690963 | MS.gene78345:CDS | 20.0% |
| !!! | TTTTGATTGATGAATTTTTC+AGG | - | chr7.1:2690943-2690962 | MS.gene78345:CDS | 20.0% |
| ! | AAAGGTGAAAAATTCAGAAA+AGG | + | chr7.1:2691080-2691099 | None:intergenic | 25.0% |
| ! | AAGGTGAAAAATTCAGAAAA+GGG | + | chr7.1:2691079-2691098 | None:intergenic | 25.0% |
| ! | AGAATTATGCTTATGGAAAT+TGG | - | chr7.1:2690516-2690535 | MS.gene78345:intron | 25.0% |
| ! | GAAATTGAACTATCTAAAGA+TGG | + | chr7.1:2690614-2690633 | None:intergenic | 25.0% |
| ! | GAAGAAAAAAAAAACATGAC+AGG | + | chr7.1:2691371-2691390 | None:intergenic | 25.0% |
| ! | TTAACAGGTTGTAATTGTAA+TGG | + | chr7.1:2690406-2690425 | None:intergenic | 25.0% |
| !! | AAAGTTCTTCTTGATTTTGA+AGG | + | chr7.1:2690466-2690485 | None:intergenic | 25.0% |
| AAGCATAATTCTCTGTTTGT+TGG | + | chr7.1:2690508-2690527 | None:intergenic | 30.0% | |
| CAAACAGAGAATTATGCTTA+TGG | - | chr7.1:2690509-2690528 | MS.gene78345:intron | 30.0% | |
| GCTAAAAAGTGGTTAGAATT+TGG | + | chr7.1:2691400-2691419 | None:intergenic | 30.0% | |
| GTGATCAAGCATCAATTATT+GGG | - | chr7.1:2690966-2690985 | MS.gene78345:intron | 30.0% | |
| GTTGAATTCTTTCCAACATT+TGG | + | chr7.1:2691570-2691589 | None:intergenic | 30.0% | |
| TGATCAAGCATCAATTATTG+GGG | - | chr7.1:2690967-2690986 | MS.gene78345:intron | 30.0% | |
| TTCAAGAGAAAGCTAAAAAG+TGG | + | chr7.1:2691411-2691430 | None:intergenic | 30.0% | |
| ! | TGCATTTTGCCTCAATATAA+AGG | + | chr7.1:2690851-2690870 | None:intergenic | 30.0% |
| !! | AAAGTAATAGAAGTACCCTT+TGG | + | chr7.1:2690796-2690815 | None:intergenic | 30.0% |
| !! | TTCTTACTTTGAGATTTGCA+TGG | + | chr7.1:2691210-2691229 | None:intergenic | 30.0% |
| !! | TTGATTTTGAAGGTTGAAGT+TGG | + | chr7.1:2690456-2690475 | None:intergenic | 30.0% |
| ACAATCATTGTTGCCACAAA+TGG | - | chr7.1:2690574-2690593 | MS.gene78345:intron | 35.0% | |
| AGTGGTTAGAATTTGGAAAG+TGG | + | chr7.1:2691393-2691412 | None:intergenic | 35.0% | |
| ATGGTCTTTGTTGAATCCAT+AGG | + | chr7.1:2690387-2690406 | None:intergenic | 35.0% | |
| ATTTGTGGCAACAATGATTG+TGG | + | chr7.1:2690575-2690594 | None:intergenic | 35.0% | |
| CTTCTATTCTATCAGCGTTA+AGG | - | chr7.1:2691330-2691349 | MS.gene78345:CDS | 35.0% | |
| GATCAAGCATCAATTATTGG+GGG | - | chr7.1:2690968-2690987 | MS.gene78345:intron | 35.0% | |
| GGTGATCAAGCATCAATTAT+TGG | - | chr7.1:2690965-2690984 | MS.gene78345:intron | 35.0% | |
| TCTTGTGGATATAGTAGAGT+TGG | + | chr7.1:2690356-2690375 | None:intergenic | 35.0% | |
| TGCTGATATTGAAGTGACAA+TGG | - | chr7.1:2691177-2691196 | MS.gene78345:CDS | 35.0% | |
| TGCTGCAAACCTTTATATTG+AGG | - | chr7.1:2690839-2690858 | MS.gene78345:CDS | 35.0% | |
| TGGTGTTCAGAAACAAAAAG+AGG | - | chr7.1:2691039-2691058 | MS.gene78345:intron | 35.0% | |
| TGTTGAATCCATAGGTGAAA+GGG | + | chr7.1:2690379-2690398 | None:intergenic | 35.0% | |
| TTGCTTCAGAATCTGTTAAC+AGG | + | chr7.1:2690421-2690440 | None:intergenic | 35.0% | |
| TTGTTGAATCCATAGGTGAA+AGG | + | chr7.1:2690380-2690399 | None:intergenic | 35.0% | |
| TTTGACACTTCATCCATTTG+TGG | + | chr7.1:2690590-2690609 | None:intergenic | 35.0% | |
| ! | ACTTGTTGAGTATTGTGGAA+AGG | + | chr7.1:2691098-2691117 | None:intergenic | 35.0% |
| !! | AAGATGGTTTCTAGTTTGCA+TGG | - | chr7.1:2691256-2691275 | MS.gene78345:CDS | 35.0% |
| !! | GAGAGTTCTGAGACAAAAAT+GGG | - | chr7.1:2691130-2691149 | MS.gene78345:intron | 35.0% |
| !! | TGAGAGTTCTGAGACAAAAA+TGG | - | chr7.1:2691129-2691148 | MS.gene78345:intron | 35.0% |
| ACCAAAACAGCTCTTGAAGA+TGG | - | chr7.1:2691240-2691259 | MS.gene78345:CDS | 40.0% | |
| AGCGTTAAGGTAATTGTCTG+TGG | - | chr7.1:2691343-2691362 | MS.gene78345:CDS | 40.0% | |
| CAGAAGCTACAATTTCTTGG+TGG | - | chr7.1:2691019-2691038 | MS.gene78345:intron | 40.0% | |
| CATCAGCTGTAGTGACATTA+AGG | + | chr7.1:2691303-2691322 | None:intergenic | 40.0% | |
| CTGCAAGAGAAACGATTCTA+AGG | + | chr7.1:2690894-2690913 | None:intergenic | 40.0% | |
| GAGCAGAAGCTACAATTTCT+TGG | - | chr7.1:2691016-2691035 | MS.gene78345:intron | 40.0% | |
| GAGGCAAAATGCAGAAATTG+CGG | - | chr7.1:2690858-2690877 | MS.gene78345:CDS | 40.0% | |
| GTTGAAGATGATTGCAAGCT+TGG | - | chr7.1:2691514-2691533 | MS.gene78345:CDS | 40.0% | |
| TAGAGAAAGAGAGAGATCCA+TGG | - | chr7.1:2690322-2690341 | MS.gene78345:CDS | 40.0% | |
| TGCAGCTGTATACCAAATGT+TGG | - | chr7.1:2691555-2691574 | MS.gene78345:CDS | 40.0% | |
| ! | ACCATCTTCAAGAGCTGTTT+TGG | + | chr7.1:2691244-2691263 | None:intergenic | 40.0% |
| ! | CTGTCACTTGTTGAGTATTG+TGG | + | chr7.1:2691103-2691122 | None:intergenic | 40.0% |
| !! | AGTTCTGAGACAAAAATGGG+TGG | - | chr7.1:2691133-2691152 | MS.gene78345:intron | 40.0% |
| !!! | TGTTTTGACTCTGCGTTTCT+TGG | + | chr7.1:2690651-2690670 | None:intergenic | 40.0% |
| ACATTGCTGTAGAACGCAAC+AGG | - | chr7.1:2690711-2690730 | MS.gene78345:CDS | 45.0% | |
| ACCCTTTGGACGTAAGATTC+AGG | + | chr7.1:2690782-2690801 | None:intergenic | 45.0% | |
| GCCTGAATCTTACGTCCAAA+GGG | - | chr7.1:2690778-2690797 | MS.gene78345:CDS | 45.0% | |
| TGAGACAAAAATGGGTGGTG+AGG | - | chr7.1:2691138-2691157 | MS.gene78345:CDS | 45.0% | |
| TGATTGCAAGCTTGGATCAG+TGG | - | chr7.1:2691522-2691541 | MS.gene78345:CDS | 45.0% | |
| TGCCTGAATCTTACGTCCAA+AGG | - | chr7.1:2690777-2690796 | MS.gene78345:CDS | 45.0% | |
| TGTGGCAACAATGATTGTGG+TGG | + | chr7.1:2690572-2690591 | None:intergenic | 45.0% | |
| !! | AACAATGATTGTGGTGGTGG+TGG | + | chr7.1:2690566-2690585 | None:intergenic | 45.0% |
| !! | TAGAGTTGGTTCTAGTGCCA+TGG | + | chr7.1:2690342-2690361 | None:intergenic | 45.0% |
| CCACAAGACCCTTTCACCTA+TGG | - | chr7.1:2690368-2690387 | MS.gene78345:CDS | 50.0% | |
| CCATAGGTGAAAGGGTCTTG+TGG | + | chr7.1:2690371-2690390 | None:intergenic | 50.0% | |
| GGCAACAATGATTGTGGTGG+TGG | + | chr7.1:2690569-2690588 | None:intergenic | 50.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr7.1 | gene | 2690315 | 2691618 | 2690315 | ID=MS.gene78345 |
| chr7.1 | mRNA | 2690315 | 2691618 | 2690315 | ID=MS.gene78345.t1;Parent=MS.gene78345 |
| chr7.1 | exon | 2691134 | 2691618 | 2691134 | ID=MS.gene78345.t1.exon1;Parent=MS.gene78345.t1 |
| chr7.1 | CDS | 2691134 | 2691618 | 2691134 | ID=cds.MS.gene78345.t1;Parent=MS.gene78345.t1 |
| chr7.1 | exon | 2690582 | 2690968 | 2690582 | ID=MS.gene78345.t1.exon2;Parent=MS.gene78345.t1 |
| chr7.1 | CDS | 2690582 | 2690968 | 2690582 | ID=cds.MS.gene78345.t1;Parent=MS.gene78345.t1 |
| chr7.1 | exon | 2690315 | 2690419 | 2690315 | ID=MS.gene78345.t1.exon3;Parent=MS.gene78345.t1 |
| chr7.1 | CDS | 2690315 | 2690419 | 2690315 | ID=cds.MS.gene78345.t1;Parent=MS.gene78345.t1 |
| Gene Sequence |
| Protein sequence |
