AlfalfaGEDB | MS.gene78378

M. sativa cultivar XinJiangDaYe / MS.gene78378

KEGG: -
GO: GO:0003674 GO:0003676 GO:0003677 GO:0005488 GO:0008270 GO:0043167 GO:0043169 GO:0046872 GO:0046914 GO:0097159 GO:1901363
PFAM: PF01428.19 PF01754.19

Nr:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene78378.t1	XP_024632562.1	97.1	172	5	0	1	172	1	172	2.70E-90	341.3

Swissprot:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene78378.t1	A2YEZ6	70.1	174	47	3	1	172	1	171	1.6e-64	246.9

Trembl:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene78378.t1	B7FGP6	97.1	172	5	0	1	172	1	172	1.9e-90	341.3

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network:

Gene1	Gene2	correlation coefficient	p_value	FDR

PPI:

Gene1	Gene2	Type
MS.gene78378	MS.gene24724	PPI
MS.gene78378	MS.gene050335	PPI

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene78378.t1	MTR_2g098160	97.093	172	5	0	1	172	1	172	1.34e-123	345
MS.gene78378.t1	MTR_0249s0070	61.765	170	61	2	3	172	4	169	2.33e-76	226
MS.gene78378.t1	MTR_0249s0070	61.765	170	61	2	3	172	12	177	2.75e-76	226
MS.gene78378.t1	MTR_7g092400	62.353	170	63	1	3	172	92	260	2.99e-76	228
MS.gene78378.t1	MTR_4g065570	77.622	143	25	2	31	172	1	137	2.96e-75	221
MS.gene78378.t1	MTR_7g104320	46.061	165	79	3	12	172	10	168	2.13e-45	147
MS.gene78378.t1	MTR_4g053440	44.444	171	82	5	8	172	7	170	3.40e-42	139
MS.gene78378.t1	MTR_2g086190	44.304	158	71	4	15	172	10	150	1.56e-40	134
MS.gene78378.t1	MTR_1g060380	44.172	163	75	4	11	172	9	156	6.69e-37	125
MS.gene78378.t1	MTR_3g028010	37.821	156	77	2	17	172	28	163	8.19e-29	104
MS.gene78378.t1	MTR_8g036980	52.222	90	29	1	83	172	76	151	1.00e-27	101
MS.gene78378.t1	MTR_1g100773	33.333	156	84	4	15	170	3	138	1.97e-25	95.5
MS.gene78378.t1	MTR_7g114920	38.065	155	69	6	15	169	3	130	1.46e-24	93.2
MS.gene78378.t1	MTR_2g054650	33.333	156	70	5	15	170	4	125	6.12e-20	80.9
MS.gene78378.t1	MTR_3g025570	70.455	44	13	0	107	150	4	47	3.79e-19	78.6

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene78378.t1	AT1G51200	61.017	177	60	4	1	172	1	173	2.29e-63	193
MS.gene78378.t1	AT1G51200	61.017	177	60	4	1	172	1	173	2.29e-63	193
MS.gene78378.t1	AT1G51200	61.017	177	60	4	1	172	1	173	2.29e-63	193
MS.gene78378.t1	AT1G51200	61.017	177	60	4	1	172	1	173	2.29e-63	193
MS.gene78378.t1	AT2G36320	47.879	165	71	4	11	172	9	161	7.40e-46	148
MS.gene78378.t1	AT4G12040	42.683	164	90	2	12	172	13	175	2.34e-45	147
MS.gene78378.t1	AT4G12040	42.683	164	90	2	12	172	13	175	2.34e-45	147
MS.gene78378.t1	AT2G27580	40.123	162	85	2	15	172	10	163	4.57e-42	138
MS.gene78378.t1	AT2G27580	40.123	162	85	2	15	172	10	163	4.57e-42	138
MS.gene78378.t1	AT1G12440	40.741	162	91	2	11	172	12	168	5.00e-40	133
MS.gene78378.t1	AT1G12440	40.741	162	91	2	11	172	12	168	5.00e-40	133
MS.gene78378.t1	AT1G12440	40.741	162	91	2	11	172	12	168	5.00e-40	133
MS.gene78378.t1	AT3G52800	42.593	162	93	0	11	172	9	170	1.01e-37	127
MS.gene78378.t1	AT3G52800	42.593	162	93	0	11	172	9	170	1.01e-37	127
MS.gene78378.t1	AT4G22820	37.888	161	94	3	15	172	19	176	1.59e-36	125
MS.gene78378.t1	AT4G22820	37.888	161	94	3	15	172	19	176	1.59e-36	125
MS.gene78378.t1	AT3G12630	39.333	150	71	2	23	172	31	160	7.56e-32	112
MS.gene78378.t1	AT4G14225	37.342	158	62	5	15	172	5	125	3.10e-27	99.8
MS.gene78378.t1	AT4G25380	32.903	155	70	4	18	172	10	130	1.59e-24	92.8

Find 47 sgRNAs with CRISPR-Local

Find 50 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
CGGTTCTGCAGAGATGGTTT+TGG	0.250126	2.3:+5147373	None:intergenic
GTTTCAGCATCATGTCTTTG+TGG	0.367335	2.3:+5147516	None:intergenic
CATCATGTCTTTGTGGCACT+TGG	0.399820	2.3:+5147523	None:intergenic
TTCCGGCTCAGAGGAGAGTT+TGG	0.400683	2.3:-5147342	MS.gene78378:CDS
CCAGCAGTGCGGTAATCAAA+TGG	0.411400	2.3:+5147191	None:intergenic
GCAGAACCGTTAGTTGCTTC+CGG	0.413723	2.3:-5147359	MS.gene78378:CDS
AATAAGCGGGTTGGTTTGAC+AGG	0.421004	2.3:-5147278	MS.gene78378:CDS
CAAAGACATGATGCTGAAAC+AGG	0.424095	2.3:-5147513	MS.gene78378:CDS
CACTTGCAGCATCTTCCATT+GGG	0.430249	2.3:-5147481	MS.gene78378:CDS
TTGCAGTTGCTGCACCGCTT+AGG	0.445309	2.3:+5147299	None:intergenic
TCCATTGGGAACATCATGAA+TGG	0.454077	2.3:-5147467	MS.gene78378:CDS
CCATTTGATTACCGCACTGC+TGG	0.477078	2.3:-5147191	MS.gene78378:CDS
ATGCACAACATAGGACCCTC+TGG	0.486214	2.3:+5147593	None:intergenic
ACACTTGCAGCATCTTCCAT+TGG	0.488087	2.3:-5147482	MS.gene78378:CDS
AGGTTCAATTACATTGCTGC+TGG	0.500525	2.3:+5147439	None:intergenic
CTTCACATCCAGTCTGATCA+TGG	0.503432	2.3:+5147621	None:intergenic
CAGCAGTGCGGTAATCAAAT+GGG	0.506125	2.3:+5147192	None:intergenic
CACCGCTTAGGACCATCCTT+CGG	0.507315	2.3:+5147311	None:intergenic
TCCACGCTGGCAGTGATAGC+AGG	0.514469	2.3:+5147419	None:intergenic
CTCCAAACTCTCCTCTGAGC+CGG	0.515481	2.3:+5147340	None:intergenic
AACTAACGGTTCTGCAGAGA+TGG	0.516940	2.3:+5147367	None:intergenic
GTGCAGCAACTGCAATAAGC+GGG	0.530850	2.3:-5147291	MS.gene78378:CDS
CCATTGGGAACATCATGAAT+GGG	0.543973	2.3:-5147466	MS.gene78378:CDS
AAAAGCAAACCCTGTTGTGA+AGG	0.563268	2.3:-5147153	MS.gene78378:CDS
GCACTTGGAACACATGTTCA+TGG	0.566316	2.3:+5147538	None:intergenic
TTGGAGAAGAAGCCGAAGGA+TGG	0.571839	2.3:-5147323	MS.gene78378:CDS
CCCATTCATGATGTTCCCAA+TGG	0.580215	2.3:+5147466	None:intergenic
TGCACAACATAGGACCCTCT+GGG	0.580560	2.3:+5147594	None:intergenic
AGCGATGTACAGCACAGTAA+AGG	0.582881	2.3:+5147234	None:intergenic
AGCAACTGCAATAAGCGGGT+TGG	0.585228	2.3:-5147287	MS.gene78378:CDS
ACAGGATTTAATTGTCGATG+TGG	0.589267	2.3:-5147260	MS.gene78378:CDS
CATGATGCTGAAACAGGAGC+AGG	0.593551	2.3:-5147507	MS.gene78378:CDS
CAGTTGTTGATGCACAACAT+AGG	0.601548	2.3:+5147584	None:intergenic
AAGATGGACCATGATCAGAC+TGG	0.612119	2.3:-5147629	MS.gene78378:CDS
GGATGTGAAGCTGCCCCAGA+GGG	0.615247	2.3:-5147608	MS.gene78378:CDS
TCTGAGCCGGAAGCAACTAA+CGG	0.631746	2.3:+5147353	None:intergenic
GAGTTTGGAGAAGAAGCCGA+AGG	0.631849	2.3:-5147327	MS.gene78378:CDS
GGTGCAGCAACTGCAATAAG+CGG	0.632084	2.3:-5147292	MS.gene78378:CDS
AATGGAAGATGCTGCAAGTG+TGG	0.642024	2.3:+5147484	None:intergenic
GTTAGTTGCTTCCGGCTCAG+AGG	0.650978	2.3:-5147351	MS.gene78378:CDS
ATCGACTGAGATCTCCACGC+TGG	0.665451	2.3:+5147406	None:intergenic
TGGATGTGAAGCTGCCCCAG+AGG	0.681846	2.3:-5147609	MS.gene78378:CDS
ACCTGCTATCACTGCCAGCG+TGG	0.686751	2.3:-5147420	MS.gene78378:CDS
AGCCGAAGGATGGTCCTAAG+CGG	0.700565	2.3:-5147313	MS.gene78378:CDS
ATGTTGTGCATCAACAACTG+TGG	0.726175	2.3:-5147581	MS.gene78378:CDS
GCACAACATAGGACCCTCTG+GGG	0.736272	2.3:+5147595	None:intergenic
TTGCATCACGTCCAGCAGTG+CGG	0.742537	2.3:+5147180	None:intergenic

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!	ATCAACAACTGTGGATTTTT+CGG	-	chr2.3:5147184-5147203	MS.gene78378:CDS	30.0%
	ACAGGATTTAATTGTCGATG+TGG	-	chr2.3:5147496-5147515	MS.gene78378:CDS	35.0%
	AAAAGCAAACCCTGTTGTGA+AGG	-	chr2.3:5147603-5147622	MS.gene78378:CDS	40.0%
	AGGTTCAATTACATTGCTGC+TGG	+	chr2.3:5147320-5147339	None:intergenic	40.0%
	ATGTTGTGCATCAACAACTG+TGG	-	chr2.3:5147175-5147194	MS.gene78378:CDS	40.0%
	CAAAGACATGATGCTGAAAC+AGG	-	chr2.3:5147243-5147262	MS.gene78378:CDS	40.0%
	CAGTTGTTGATGCACAACAT+AGG	+	chr2.3:5147175-5147194	None:intergenic	40.0%
	CCATTGGGAACATCATGAAT+GGG	-	chr2.3:5147290-5147309	MS.gene78378:CDS	40.0%
	GTTTCAGCATCATGTCTTTG+TGG	+	chr2.3:5147243-5147262	None:intergenic	40.0%
	TCCATTGGGAACATCATGAA+TGG	-	chr2.3:5147289-5147308	MS.gene78378:CDS	40.0%
	AACTAACGGTTCTGCAGAGA+TGG	+	chr2.3:5147392-5147411	None:intergenic	45.0%
	AATGGAAGATGCTGCAAGTG+TGG	+	chr2.3:5147275-5147294	None:intergenic	45.0%
	ACACTTGCAGCATCTTCCAT+TGG	-	chr2.3:5147274-5147293	MS.gene78378:CDS	45.0%
	AGCGATGTACAGCACAGTAA+AGG	+	chr2.3:5147525-5147544	None:intergenic	45.0%
	CACTTGCAGCATCTTCCATT+GGG	-	chr2.3:5147275-5147294	MS.gene78378:CDS	45.0%
	CAGCAGTGCGGTAATCAAAT+GGG	+	chr2.3:5147567-5147586	None:intergenic	45.0%
	CATCATGTCTTTGTGGCACT+TGG	+	chr2.3:5147236-5147255	None:intergenic	45.0%
	CCCATTCATGATGTTCCCAA+TGG	+	chr2.3:5147293-5147312	None:intergenic	45.0%
	CTTCACATCCAGTCTGATCA+TGG	+	chr2.3:5147138-5147157	None:intergenic	45.0%
	GCACTTGGAACACATGTTCA+TGG	+	chr2.3:5147221-5147240	None:intergenic	45.0%
!	AGCTTTTCAGCCTTCACAAC+AGG	+	chr2.3:5147616-5147635	None:intergenic	45.0%
!	GCTTTTCAGCCTTCACAACA+GGG	+	chr2.3:5147615-5147634	None:intergenic	45.0%
!	GGTTCTGCAGAGATGGTTTT+GGG	+	chr2.3:5147385-5147404	None:intergenic	45.0%
!!	AATAAGCGGGTTGGTTTGAC+AGG	-	chr2.3:5147478-5147497	MS.gene78378:CDS	45.0%
	AGCAACTGCAATAAGCGGGT+TGG	-	chr2.3:5147469-5147488	MS.gene78378:CDS	50.0%
	ATGCACAACATAGGACCCTC+TGG	+	chr2.3:5147166-5147185	None:intergenic	50.0%
	CATGATGCTGAAACAGGAGC+AGG	-	chr2.3:5147249-5147268	MS.gene78378:CDS	50.0%
	CCAGCAGTGCGGTAATCAAA+TGG	+	chr2.3:5147568-5147587	None:intergenic	50.0%
	CCATTTGATTACCGCACTGC+TGG	-	chr2.3:5147565-5147584	MS.gene78378:CDS	50.0%
	GAGTTTGGAGAAGAAGCCGA+AGG	-	chr2.3:5147429-5147448	MS.gene78378:CDS	50.0%
	GCAGAACCGTTAGTTGCTTC+CGG	-	chr2.3:5147397-5147416	MS.gene78378:CDS	50.0%
	GGTGCAGCAACTGCAATAAG+CGG	-	chr2.3:5147464-5147483	MS.gene78378:CDS	50.0%
	GTGCAGCAACTGCAATAAGC+GGG	-	chr2.3:5147465-5147484	MS.gene78378:CDS	50.0%
	TCTGAGCCGGAAGCAACTAA+CGG	+	chr2.3:5147406-5147425	None:intergenic	50.0%
	TGCACAACATAGGACCCTCT+GGG	+	chr2.3:5147165-5147184	None:intergenic	50.0%
	TTGGAGAAGAAGCCGAAGGA+TGG	-	chr2.3:5147433-5147452	MS.gene78378:CDS	50.0%
!	CGGTTCTGCAGAGATGGTTT+TGG	+	chr2.3:5147386-5147405	None:intergenic	50.0%
	AGCCGAAGGATGGTCCTAAG+CGG	-	chr2.3:5147443-5147462	MS.gene78378:CDS	55.0%
	ATCGACTGAGATCTCCACGC+TGG	+	chr2.3:5147353-5147372	None:intergenic	55.0%
	CACCGCTTAGGACCATCCTT+CGG	+	chr2.3:5147448-5147467	None:intergenic	55.0%
	CTCCAAACTCTCCTCTGAGC+CGG	+	chr2.3:5147419-5147438	None:intergenic	55.0%
	GCACAACATAGGACCCTCTG+GGG	+	chr2.3:5147164-5147183	None:intergenic	55.0%
	GTTAGTTGCTTCCGGCTCAG+AGG	-	chr2.3:5147405-5147424	MS.gene78378:CDS	55.0%
	TTCCGGCTCAGAGGAGAGTT+TGG	-	chr2.3:5147414-5147433	MS.gene78378:CDS	55.0%
	TTGCAGTTGCTGCACCGCTT+AGG	+	chr2.3:5147460-5147479	None:intergenic	55.0%
	TTGCATCACGTCCAGCAGTG+CGG	+	chr2.3:5147579-5147598	None:intergenic	55.0%
	ACCTGCTATCACTGCCAGCG+TGG	-	chr2.3:5147336-5147355	MS.gene78378:CDS	60.0%
	GGATGTGAAGCTGCCCCAGA+GGG	-	chr2.3:5147148-5147167	MS.gene78378:CDS	60.0%
	TCCACGCTGGCAGTGATAGC+AGG	+	chr2.3:5147340-5147359	None:intergenic	60.0%
	TGGATGTGAAGCTGCCCCAG+AGG	-	chr2.3:5147147-5147166	MS.gene78378:CDS	60.0%

Chromosome	Type	Strat	End	Strand	Name
chr2.3	gene	5147130	5147648	5147130	ID=MS.gene78378
chr2.3	mRNA	5147130	5147648	5147130	ID=MS.gene78378.t1;Parent=MS.gene78378
chr2.3	exon	5147130	5147648	5147130	ID=MS.gene78378.t1.exon1;Parent=MS.gene78378.t1
chr2.3	CDS	5147130	5147648	5147130	ID=cds.MS.gene78378.t1;Parent=MS.gene78378.t1

Gene Sequence

>MS.gene78378

ATGGACCATGATCAGACTGGATGTGAAGCTGCCCCAGAGGGTCCTATGTTGTGCATCAACAACTGTGGATTTTTCGGAAGCGCAGCTACCATGAACATGTGTTCCAAGTGCCACAAAGACATGATGCTGAAACAGGAGCAGGCCACACTTGCAGCATCTTCCATTGGGAACATCATGAATGGGTCATCCAGCAGCAATGTAATTGAACCTGCTATCACTGCCAGCGTGGAGATCTCAGTCGATCAAGTAGAGCCCAAAACCATCTCTGCAGAACCGTTAGTTGCTTCCGGCTCAGAGGAGAGTTTGGAGAAGAAGCCGAAGGATGGTCCTAAGCGGTGCAGCAACTGCAATAAGCGGGTTGGTTTGACAGGATTTAATTGTCGATGTGGTAACCTTTACTGTGCTGTACATCGCTACTCAGACAAGCATGACTGCCCATTTGATTACCGCACTGCTGGACGTGATGCAATAGCAAAAGCAAACCCTGTTGTGAAGGCTGAAAAGCTTGACAAGATCTAG

Protein sequence

>MS.gene78378.t1

MDHDQTGCEAAPEGPMLCINNCGFFGSAATMNMCSKCHKDMMLKQEQATLAASSIGNIMNGSSSSNVIEPAITASVEISVDQVEPKTISAEPLVASGSEESLEKKPKDGPKRCSNCNKRVGLTGFNCRCGNLYCAVHRYSDKHDCPFDYRTAGRDAIAKANPVVKAEKLDKI