AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene78400

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene78400.t1 MTR_2g097800 87.385 325 23 5 1 311 1 321 0.0 551
MS.gene78400.t1 MTR_4g064947 39.695 262 133 6 73 311 118 377 1.25e-46 161
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene78400.t1 AT5G59050 34.483 319 119 15 25 311 52 312 2.44e-21 92.4
MS.gene78400.t1 AT5G59050 38.119 202 67 13 145 311 13 191 6.60e-12 63.9

Find 70 sgRNAs with CRISPR-Local

Find 213 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
ACCTACGCTAACGTGGTTAA+AGG 0.245389 2.3:-5366117 MS.gene78400:CDS
AAATTAAAGCCATTGAATTA+AGG 0.289984 2.3:-5365966 MS.gene78400:CDS
GCACAACTCTTGCTGGTATA+AGG 0.306651 2.3:+5364404 None:intergenic
CCTACGCTAACGTGGTTAAA+GGG 0.390281 2.3:-5366116 MS.gene78400:CDS
TGATGAAAATCTATCAATAT+TGG 0.390503 2.3:+5366011 None:intergenic
AAAGCCTGCACAACTCTTGC+TGG 0.391708 2.3:+5364397 None:intergenic
ACCTGTCCCACTTCTTGAAC+CGG 0.402912 2.3:+5365792 None:intergenic
CTCCGCCAGCTACACCTTGT+TGG 0.404982 2.3:-5366149 MS.gene78400:CDS
GGAGGGACGCCAACAACTTT+GGG 0.411943 2.3:+5364349 None:intergenic
GAGGCAGAATCATATCATTC+TGG 0.418235 2.3:+5362665 None:intergenic
GTCTTCCAACAAGGTGTAGC+TGG 0.435910 2.3:+5366144 None:intergenic
TGGAGGGACGCCAACAACTT+TGG 0.438518 2.3:+5364348 None:intergenic
GCAACTACACTTTGACAAAA+CGG 0.470788 2.3:-5364374 MS.gene78400:CDS
CTGTCTCAGAAGCAAGGTAA+TGG 0.472108 2.3:-5365910 MS.gene78400:CDS
CCACTGGTCTGCAATGGAGC+AGG 0.476520 2.3:+5365850 None:intergenic
CCAGTTCGGAATTGTGACTC+TGG 0.478083 2.3:+5366377 None:intergenic
ATATGATTCTGCCTCAAGAA+TGG 0.490516 2.3:-5362657 MS.gene78400:CDS
ATGACCCTTCTGAGCTCCCT+TGG 0.499706 2.3:+5366184 None:intergenic
TATACCAGCAAGAGTTGTGC+AGG 0.500359 2.3:-5364401 MS.gene78400:CDS
GCTCCATTGCAGACCAGTGG+TGG 0.500459 2.3:-5365847 MS.gene78400:CDS
CTGCATCATTCCTCCACCAC+TGG 0.505752 2.3:+5365834 None:intergenic
GTGGGATTTAATTAGTTCAC+CGG 0.508002 2.3:-5366238 MS.gene78400:CDS
TGTAACCAAGGGAGCTCAGA+AGG 0.515050 2.3:-5366189 MS.gene78400:CDS
CCAGAGTCACAATTCCGAAC+TGG 0.519755 2.3:-5366377 MS.gene78400:intron
TCCGGTTCAAGAAGTGGGAC+AGG 0.522917 2.3:-5365793 MS.gene78400:CDS
CCTCCACCACTGGTCTGCAA+TGG 0.524420 2.3:+5365844 None:intergenic
ATCACACAAAGCATTGATTG+AGG 0.524445 2.3:-5365992 MS.gene78400:CDS
TGAAAATCTATCAATATTGG+AGG 0.528441 2.3:+5366014 None:intergenic
CCATTGCAGACCAGTGGTGG+AGG 0.531905 2.3:-5365844 MS.gene78400:CDS
AAAACGGCTCCCAAAGTTGT+TGG 0.534159 2.3:-5364358 MS.gene78400:CDS
GGTGGGTCCGGTTCAAGAAG+TGG 0.535246 2.3:-5365799 MS.gene78400:CDS
GTGAAAACATACCTTCCCTA+TGG 0.540528 2.3:+5362725 None:intergenic
CACCATGGTTCCAACGGACG+AGG 0.546751 2.3:-5366488 MS.gene78400:CDS
GGAAACATATGGTACCAGTT+CGG 0.547360 2.3:+5366363 None:intergenic
ATCCTCGTCCGTTGGAACCA+TGG 0.552840 2.3:+5366486 None:intergenic
CTCCCTTGGTTACAACTCAC+CGG 0.562393 2.3:+5366198 None:intergenic
CAGTTCGGAATTGTGACTCT+GGG 0.562437 2.3:+5366378 None:intergenic
CAGTGGTGGAGGAATGATGC+AGG 0.565231 2.3:-5365833 MS.gene78400:CDS
GCGACTTCTAAACAGAATCA+TGG 0.566085 2.3:-5366060 MS.gene78400:CDS
GAATGGTACCACGGAGTGGA+GGG 0.567764 2.3:+5364332 None:intergenic
CTTCAATATGTCCATTCTTG+AGG 0.572811 2.3:+5362646 None:intergenic
CAATCACACCATGGTTCCAA+CGG 0.579340 2.3:-5366494 MS.gene78400:CDS
GGATTCAACTCTGTGGTCAC+CGG 0.580045 2.3:-5366217 MS.gene78400:CDS
GTGATGAAATCCTCGTCCGT+TGG 0.582382 2.3:+5366478 None:intergenic
TCTCAGAAGCAAGGTAATGG+AGG 0.589606 2.3:-5365907 MS.gene78400:CDS
GGTGACCACAGAGTTGAATC+CGG 0.590456 2.3:+5366219 None:intergenic
CTACGCTAACGTGGTTAAAG+GGG 0.595945 2.3:-5366115 MS.gene78400:CDS
GTGGGTCCGGTTCAAGAAGT+GGG 0.596347 2.3:-5365798 MS.gene78400:CDS
AGAATGGTACCACGGAGTGG+AGG 0.600926 2.3:+5364331 None:intergenic
CCCTTTAACCACGTTAGCGT+AGG 0.602141 2.3:+5366116 None:intergenic
CTCAGAAGCAAGGTAATGGA+GGG 0.607872 2.3:-5365906 MS.gene78400:CDS
AGACATTTGATGAGTTAACT+CGG 0.619910 2.3:+5366456 None:intergenic
TGATTCTGATGGAGCAACAA+CGG 0.623497 2.3:+5365747 None:intergenic
GGCCTTGTTAGATGAGGACG+AGG 0.624372 2.3:+5365871 None:intergenic
CCTGCTCCATTGCAGACCAG+TGG 0.625104 2.3:-5365850 MS.gene78400:CDS
CACCGGTGAGTTGTAACCAA+GGG 0.632229 2.3:-5366200 MS.gene78400:CDS
GCAACAACGGTTCCAGCACG+AGG 0.632333 2.3:+5365760 None:intergenic
TTCGGAATTGTGACTCTGGG+AGG 0.633765 2.3:+5366381 None:intergenic
TTCCAACAAGGTGTAGCTGG+CGG 0.645177 2.3:+5366147 None:intergenic
GGAGCAGGCCTTGTTAGATG+AGG 0.651849 2.3:+5365865 None:intergenic
CATGCTCTGTCTCAGAAGCA+AGG 0.652288 2.3:-5365916 MS.gene78400:CDS
GTCCTCGTCCTCATCTAACA+AGG 0.653193 2.3:-5365873 MS.gene78400:CDS
TTAAGAATGGTACCACGGAG+TGG 0.655116 2.3:+5364328 None:intergenic
GTAACCAAGGGAGCTCAGAA+GGG 0.662619 2.3:-5366188 MS.gene78400:CDS
TCTTGCTGGTATAAGGACAG+TGG 0.678497 2.3:+5364411 None:intergenic
GTTGGCGTCCCTCCACTCCG+TGG 0.686225 2.3:-5364340 MS.gene78400:intron
TGCTAACTGAGAGAACATCA+TGG 0.694654 2.3:+5366521 None:intergenic
TCACCGGTGAGTTGTAACCA+AGG 0.715334 2.3:-5366201 MS.gene78400:CDS
GTTCACCGGATTCAACTCTG+TGG 0.734801 2.3:-5366224 MS.gene78400:CDS
TAGCAACAACAATCACACCA+TGG 0.741419 2.3:-5366503 MS.gene78400:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TTTTTTTTTTTAATTTAAAT+CGG + chr2.3:5365175-5365194 None:intergenic 0.0%
!! CAGTTAAATATAATTAATTA+AGG + chr2.3:5364674-5364693 None:intergenic 10.0%
!! TAACAATAATAAATTAAGAA+TGG + chr2.3:5364880-5364899 None:intergenic 10.0%
!!! AATTTTAAATGCATATAAAA+TGG + chr2.3:5365279-5365298 None:intergenic 10.0%
!!! ATATGCATTTAAAATTTATT+TGG - chr2.3:5365283-5365302 MS.gene78400:intron 10.0%
!!! ATATTTGATGTTTTTAAAAT+TGG + chr2.3:5363959-5363978 None:intergenic 10.0%
!!! ATTTTAAATGCATATAAAAT+GGG + chr2.3:5365278-5365297 None:intergenic 10.0%
!!! CAATTTTAATAGATTTAAAA+TGG - chr2.3:5363900-5363919 MS.gene78400:intron 10.0%
!!! CTTTCTTTTTAAAATTATTT+GGG - chr2.3:5365374-5365393 MS.gene78400:intron 10.0%
!!! TAATTTTAAAGATATTTACA+TGG - chr2.3:5363671-5363690 MS.gene78400:intron 10.0%
!!! TATGCATTTAAAATTTATTT+GGG - chr2.3:5365284-5365303 MS.gene78400:intron 10.0%
!! AAATTAAACAAATGTAGAAA+GGG + chr2.3:5365972-5365991 None:intergenic 15.0%
!! ATTTATAAACAATTTCTACA+TGG - chr2.3:5365525-5365544 MS.gene78400:intron 15.0%
!! GGTAAGATTATTTATTTATT+AGG + chr2.3:5364977-5364996 None:intergenic 15.0%
!! TAAATTAAACAAATGTAGAA+AGG + chr2.3:5365973-5365992 None:intergenic 15.0%
!!! AACAATGTTAAATCTTTTTA+AGG - chr2.3:5365079-5365098 MS.gene78400:intron 15.0%
!!! ACTTAAGAGTATTTTATTTT+TGG + chr2.3:5365435-5365454 None:intergenic 15.0%
!!! ATACTTTTCTTTAGAAAAAT+TGG - chr2.3:5366107-5366126 MS.gene78400:CDS 15.0%
!!! CCAAATAATTTTAAAAAGAA+AGG + chr2.3:5365376-5365395 None:intergenic 15.0%
!!! CCTTTCTTTTTAAAATTATT+TGG - chr2.3:5365373-5365392 MS.gene78400:intron 15.0%
!!! GATAGGTTTTTTTTTTTTTA+AGG + chr2.3:5364413-5364432 None:intergenic 15.0%
!!! TTTTATATTATAGAACTAGT+TGG + chr2.3:5366343-5366362 None:intergenic 15.0%
!! AAACTTAGAGTATAAAACTT+AGG + chr2.3:5365405-5365424 None:intergenic 20.0%
!! AAATTAAAGCCATTGAATTA+AGG - chr2.3:5363226-5363245 MS.gene78400:intron 20.0%
!! CAAAGAAGAGTTAATAATAT+AGG + chr2.3:5362908-5362927 None:intergenic 20.0%
!! GATTGATAAGAAAATGAAAT+AGG + chr2.3:5365499-5365518 None:intergenic 20.0%
!! TGATGAAAATCTATCAATAT+TGG + chr2.3:5363184-5363203 None:intergenic 20.0%
!! TTATATATATTGTTAGGGAA+AGG - chr2.3:5364753-5364772 MS.gene78400:intron 20.0%
!!! AAACGAATTTGATATTGTTT+TGG + chr2.3:5364697-5364716 None:intergenic 20.0%
!!! AAATGAAAGCTTTTTTTTTG+TGG - chr2.3:5364151-5364170 MS.gene78400:intron 20.0%
!!! ATGTTTTTTCCTTAATTCAA+TGG + chr2.3:5363238-5363257 None:intergenic 20.0%
!!! TGATTTGCAATTCTTAATAA+TGG - chr2.3:5366229-5366248 MS.gene78400:CDS 20.0%
!!! TTTGATGTTTTTAAAATTGG+AGG + chr2.3:5363956-5363975 None:intergenic 20.0%
! AAAAATAAAACTGAACCGAA+AGG + chr2.3:5364569-5364588 None:intergenic 25.0%
! AACTAGATTGATGCTATTAA+GGG - chr2.3:5364077-5364096 MS.gene78400:intron 25.0%
! AATTAAGCAGTTAGAAAGTT+TGG - chr2.3:5364611-5364630 MS.gene78400:intron 25.0%
! ACAGATTCGAATATTAAAAG+TGG + chr2.3:5363573-5363592 None:intergenic 25.0%
! ACCAAAAATATTCTTTATGG+TGG - chr2.3:5364480-5364499 MS.gene78400:intron 25.0%
! ATAAATTAAGAATGGTACCA+CGG + chr2.3:5364872-5364891 None:intergenic 25.0%
! CAGATTCGAATATTAAAAGT+GGG + chr2.3:5363572-5363591 None:intergenic 25.0%
! GCACGTTATATATATTGTTA+GGG - chr2.3:5364748-5364767 MS.gene78400:intron 25.0%
! GTGACCAAAAATATTCTTTA+TGG - chr2.3:5364477-5364496 MS.gene78400:intron 25.0%
! GTTATTCAATAAACCTGAAA+AGG + chr2.3:5362873-5362892 None:intergenic 25.0%
! TAACTAGATTGATGCTATTA+AGG - chr2.3:5364076-5364095 MS.gene78400:intron 25.0%
! TGAAAATCTATCAATATTGG+AGG + chr2.3:5363181-5363200 None:intergenic 25.0%
! TGCACGTTATATATATTGTT+AGG - chr2.3:5364747-5364766 MS.gene78400:intron 25.0%
! TTTCTACATGGAAAATTCTA+CGG - chr2.3:5365537-5365556 MS.gene78400:intron 25.0%
!! AAACTTAGAGCATTAAACTT+AGG + chr2.3:5365315-5365334 None:intergenic 25.0%
!! ACCACCATAAAGAATATTTT+TGG + chr2.3:5364484-5364503 None:intergenic 25.0%
!! TAAGTTTAATGCTCTAAGTT+TGG - chr2.3:5365314-5365333 MS.gene78400:intron 25.0%
!! TACAGGTACTATTTTATTGT+CGG + chr2.3:5365809-5365828 None:intergenic 25.0%
AAGCTTGTAGAGTTACAAAT+GGG + chr2.3:5363930-5363949 None:intergenic 30.0%
ACTAACATCTCGAATAGATT+AGG + chr2.3:5365127-5365146 None:intergenic 30.0%
ACTAATAAAGAAGACACTTG+AGG + chr2.3:5363766-5363785 None:intergenic 30.0%
AGACATTTGATGAGTTAACT+CGG + chr2.3:5362739-5362758 None:intergenic 30.0%
AGTACAAAGACAAGATAGAT+AGG + chr2.3:5364430-5364449 None:intergenic 30.0%
ATATTGCAAGCATTACTAGA+TGG - chr2.3:5365837-5365856 MS.gene78400:CDS 30.0%
ATCTTACCCAACAAAATATG+TGG - chr2.3:5364989-5365008 MS.gene78400:intron 30.0%
ATGTTTATGTGACAACTTAG+TGG + chr2.3:5364299-5364318 None:intergenic 30.0%
CAAACAGATGAGAAATTGAT+AGG + chr2.3:5366011-5366030 None:intergenic 30.0%
CAGCATGTTACTATAGATTA+GGG + chr2.3:5364521-5364540 None:intergenic 30.0%
CCAAGTTAACTAAAAGTCTT+AGG - chr2.3:5365033-5365052 MS.gene78400:intron 30.0%
CGAATAGATTAGGATTACAA+TGG + chr2.3:5365117-5365136 None:intergenic 30.0%
GATGACATGACATATTTAGA+TGG + chr2.3:5364277-5364296 None:intergenic 30.0%
GCAGTCTCGAAAAATAAAAT+TGG + chr2.3:5366269-5366288 None:intergenic 30.0%
GTTTGTGCAATTATTCGTAT+AGG - chr2.3:5365145-5365164 MS.gene78400:intron 30.0%
TAAGCTTGTAGAGTTACAAA+TGG + chr2.3:5363931-5363950 None:intergenic 30.0%
TAGATAGTAAGGGAAACATA+TGG + chr2.3:5362843-5362862 None:intergenic 30.0%
TAGTAATGCTTGCAATATCT+TGG + chr2.3:5365835-5365854 None:intergenic 30.0%
TATTGAATGTCATGTTGCTA+AGG + chr2.3:5365232-5365251 None:intergenic 30.0%
TCAGAATCAACCAAAAAGAA+AGG - chr2.3:5363459-5363478 MS.gene78400:intron 30.0%
TCTTAAGTGTTACACTTTGT+GGG + chr2.3:5365744-5365763 None:intergenic 30.0%
TTAGGAGAGTAGTAACTAAA+CGG + chr2.3:5364959-5364978 None:intergenic 30.0%
! AAATCAAAGTTGTTGCTTGA+TGG + chr2.3:5364909-5364928 None:intergenic 30.0%
! AGTCATGAGAATGACTATTT+TGG - chr2.3:5365563-5365582 MS.gene78400:intron 30.0%
! ATCTCATCCACATATTTTGT+TGG + chr2.3:5364999-5365018 None:intergenic 30.0%
! GATGAATTGATGATACTATG+AGG + chr2.3:5365664-5365683 None:intergenic 30.0%
! TCTCATCCACATATTTTGTT+GGG + chr2.3:5364998-5365017 None:intergenic 30.0%
! TGTTCATGTTTTTCACTAGT+CGG - chr2.3:5363508-5363527 MS.gene78400:intron 30.0%
!! AGTTGATGATTTGTTCCTTT+CGG - chr2.3:5364551-5364570 MS.gene78400:intron 30.0%
!! CCTAAGACTTTTAGTTAACT+TGG + chr2.3:5365036-5365055 None:intergenic 30.0%
!!! AGATCTTGTTTTGAACCATA+GGG - chr2.3:5366452-5366471 MS.gene78400:CDS 30.0%
!!! TGAAAGCTTTTTTTTTGTGG+TGG - chr2.3:5364154-5364173 MS.gene78400:intron 30.0%
!!! TTCTTTTTGGTTGATTCTGA+TGG + chr2.3:5363459-5363478 None:intergenic 30.0%
AACCAAAAAGAAAGGTAACG+TGG - chr2.3:5363467-5363486 MS.gene78400:intron 35.0%
AGGATGAAGCAACAAATTCA+TGG + chr2.3:5365212-5365231 None:intergenic 35.0%
ATATGATTCTGCCTCAAGAA+TGG - chr2.3:5366535-5366554 MS.gene78400:CDS 35.0%
ATCCACCTATATGCTATTGA+GGG - chr2.3:5363982-5364001 MS.gene78400:intron 35.0%
ATTCCAACAGTCCAAATACT+CGG - chr2.3:5366297-5366316 MS.gene78400:intron 35.0%
CTTAAGTGTTACACTTTGTG+GGG + chr2.3:5365743-5365762 None:intergenic 35.0%
CTTAGAAAGTAGAAACAGAG+TGG + chr2.3:5363620-5363639 None:intergenic 35.0%
GCAACTACACTTTGACAAAA+CGG - chr2.3:5364818-5364837 MS.gene78400:intron 35.0%
GCAGCATGTTACTATAGATT+AGG + chr2.3:5364522-5364541 None:intergenic 35.0%
GTCTTAAGTGTTACACTTTG+TGG + chr2.3:5365745-5365764 None:intergenic 35.0%
GTGCATAAGATATGCAAGAT+CGG + chr2.3:5364194-5364213 None:intergenic 35.0%
GTGGGATTTAATTAGTTCAC+CGG - chr2.3:5362954-5362973 MS.gene78400:intron 35.0%
TATCCACCTATATGCTATTG+AGG - chr2.3:5363981-5364000 MS.gene78400:intron 35.0%
TGATAGGTGAAATCCTAAAG+TGG - chr2.3:5364021-5364040 MS.gene78400:intron 35.0%
TGCATAAGATATGCAAGATC+GGG + chr2.3:5364193-5364212 None:intergenic 35.0%
TGTTACGCTTATAACAAGGT+AGG + chr2.3:5365720-5365739 None:intergenic 35.0%
TTAGGATTTCACCTATCACA+TGG + chr2.3:5364019-5364038 None:intergenic 35.0%
TTGCAATATCTTGGACTTAC+AGG + chr2.3:5365826-5365845 None:intergenic 35.0%
! ATCCACGTTACCTTTCTTTT+TGG + chr2.3:5363472-5363491 None:intergenic 35.0%
! TAGGTTTTTGTCTTCCAACA+AGG + chr2.3:5363060-5363079 None:intergenic 35.0%
! TGTACTAGTTTGCATGTGAT+CGG - chr2.3:5364444-5364463 MS.gene78400:intron 35.0%
! TGTTTTTCACTAGTCGGTAA+TGG - chr2.3:5363514-5363533 MS.gene78400:intron 35.0%
!! ATCAATCTAGTTGCCACTTT+AGG + chr2.3:5364037-5364056 None:intergenic 35.0%
!! ATCACACAAAGCATTGATTG+AGG - chr2.3:5363200-5363219 MS.gene78400:intron 35.0%
!!! AGCTTTTTTTTTGTGGTGGT+CGG - chr2.3:5364158-5364177 MS.gene78400:intron 35.0%
!!! CAGATCTTGTTTTGAACCAT+AGG - chr2.3:5366451-5366470 MS.gene78400:CDS 35.0%
!!! TGCTTTTTCTACCATGTGAT+AGG - chr2.3:5364005-5364024 MS.gene78400:intron 35.0%
AAGCACCCTCAATAGCATAT+AGG + chr2.3:5363990-5364009 None:intergenic 40.0%
AATCCCACGACTACATGAAA+AGG + chr2.3:5362942-5362961 None:intergenic 40.0%
ACACCTCACTGTTTGTACAA+TGG - chr2.3:5365623-5365642 MS.gene78400:intron 40.0%
ATACCGAGTATTTGGACTGT+TGG + chr2.3:5366303-5366322 None:intergenic 40.0%
ATGCCATTGTACAAACAGTG+AGG + chr2.3:5365629-5365648 None:intergenic 40.0%
CTAGCTAGATAGGCTAAATG+TGG + chr2.3:5364384-5364403 None:intergenic 40.0%
GAGGCAGAATCATATCATTC+TGG + chr2.3:5366530-5366549 None:intergenic 40.0%
GCATAAGATATGCAAGATCG+GGG + chr2.3:5364192-5364211 None:intergenic 40.0%
GCGACTTCTAAACAGAATCA+TGG - chr2.3:5363132-5363151 MS.gene78400:intron 40.0%
GGAAACATATGGTACCAGTT+CGG + chr2.3:5362832-5362851 None:intergenic 40.0%
GGCTAAATGTGGATGATTAG+TGG + chr2.3:5364373-5364392 None:intergenic 40.0%
GTGAAAACATACCTTCCCTA+TGG + chr2.3:5366470-5366489 None:intergenic 40.0%
TAGCAACAACAATCACACCA+TGG - chr2.3:5362689-5362708 MS.gene78400:CDS 40.0%
TGATTCTGATGGAGCAACAA+CGG + chr2.3:5363448-5363467 None:intergenic 40.0%
TGCTAACTGAGAGAACATCA+TGG + chr2.3:5362674-5362693 None:intergenic 40.0%
! CTTCCTTTTCATGTAGTCGT+GGG - chr2.3:5362936-5362955 MS.gene78400:intron 40.0%
! GCTAGTTGATACCGAGTATT+TGG + chr2.3:5366311-5366330 None:intergenic 40.0%
! TCTTCCTTTTCATGTAGTCG+TGG - chr2.3:5362935-5362954 MS.gene78400:intron 40.0%
! TTTCACTAGTCGGTAATGGT+TGG - chr2.3:5363518-5363537 MS.gene78400:intron 40.0%
!!! CTTGTTTTGAACCATAGGGA+AGG - chr2.3:5366456-5366475 MS.gene78400:CDS 40.0%
!!! CTTTTTTTTTGTGGTGGTCG+GGG - chr2.3:5364160-5364179 MS.gene78400:intron 40.0%
!!! GCTTTTTTTTTGTGGTGGTC+GGG - chr2.3:5364159-5364178 MS.gene78400:intron 40.0%
AAAACGGCTCCCAAAGTTGT+TGG - chr2.3:5364834-5364853 MS.gene78400:intron 45.0%
AAGGAACGAGCTAGCTAGAT+AGG + chr2.3:5364394-5364413 None:intergenic 45.0%
ACCTACGCTAACGTGGTTAA+AGG - chr2.3:5363075-5363094 MS.gene78400:intron 45.0%
CAATCACACCATGGTTCCAA+CGG - chr2.3:5362698-5362717 MS.gene78400:CDS 45.0%
CACCCTCAATAGCATATAGG+TGG + chr2.3:5363987-5364006 None:intergenic 45.0%
CAGTTCGGAATTGTGACTCT+GGG + chr2.3:5362817-5362836 None:intergenic 45.0%
CCTACGCTAACGTGGTTAAA+GGG - chr2.3:5363076-5363095 MS.gene78400:intron 45.0%
CTACGCTAACGTGGTTAAAG+GGG - chr2.3:5363077-5363096 MS.gene78400:intron 45.0%
CTGTCTCAGAAGCAAGGTAA+TGG - chr2.3:5363282-5363301 MS.gene78400:intron 45.0%
GACAAAAACCTACGCTAACG+TGG - chr2.3:5363068-5363087 MS.gene78400:intron 45.0%
GCACAACTCTTGCTGGTATA+AGG + chr2.3:5364791-5364810 None:intergenic 45.0%
TATACCAGCAAGAGTTGTGC+AGG - chr2.3:5364791-5364810 MS.gene78400:intron 45.0%
TCTTGCTGGTATAAGGACAG+TGG + chr2.3:5364784-5364803 None:intergenic 45.0%
TTAAGAATGGTACCACGGAG+TGG + chr2.3:5364867-5364886 None:intergenic 45.0%
! AAGGAAGCGCGTAGATAGTA+AGG + chr2.3:5362854-5362873 None:intergenic 45.0%
! AGGAAGCGCGTAGATAGTAA+GGG + chr2.3:5362853-5362872 None:intergenic 45.0%
! CTAGTTTGCATGTGATCGGA+TGG - chr2.3:5364448-5364467 MS.gene78400:intron 45.0%
! GGGGTGTTACGCTTATAACA+AGG + chr2.3:5365724-5365743 None:intergenic 45.0%
! TAGTCGGTAATGGTTGGTTG+CGG - chr2.3:5363524-5363543 MS.gene78400:intron 45.0%
! TATCTACGCGCTTCCTTTTC+AGG - chr2.3:5362857-5362876 MS.gene78400:intron 45.0%
! TCTCAGAAGCAAGGTAATGG+AGG - chr2.3:5363285-5363304 MS.gene78400:intron 45.0%
! TGGTTGGTTGCGGAATCAAA+TGG - chr2.3:5363534-5363553 MS.gene78400:intron 45.0%
!! CTCAGAAGCAAGGTAATGGA+GGG - chr2.3:5363286-5363305 MS.gene78400:intron 45.0%
!!! ATGATGCAGGCCGTTTTTCT+TGG - chr2.3:5363372-5363391 MS.gene78400:intron 45.0%
!!! CTGTTGCTGCTTTTGTTGAC+TGG + chr2.3:5366503-5366522 None:intergenic 45.0%
!! AAAAAAAAAAAATACATTTA+AGG + chr2.3:5363842-5363861 None:intergenic 5.0%
!!! ATATCTTTAAAATTATTTAT+TGG + chr2.3:5363666-5363685 None:intergenic 5.0%
!!! TATCTTTAAAATTATTTATT+GGG + chr2.3:5363665-5363684 None:intergenic 5.0%
!!! TTTTTTTTTTAATTTAAATC+GGG + chr2.3:5365174-5365193 None:intergenic 5.0%
AAAGCCTGCACAACTCTTGC+TGG + chr2.3:5364798-5364817 None:intergenic 50.0%
ACCGGACCCACCAAGAAAAA+CGG + chr2.3:5363385-5363404 None:intergenic 50.0%
ACCTGTCCCACTTCTTGAAC+CGG + chr2.3:5363403-5363422 None:intergenic 50.0%
CACCGGTGAGTTGTAACCAA+GGG - chr2.3:5362992-5363011 MS.gene78400:intron 50.0%
CATGCTCTGTCTCAGAAGCA+AGG - chr2.3:5363276-5363295 MS.gene78400:intron 50.0%
CCAGAGTCACAATTCCGAAC+TGG - chr2.3:5362815-5362834 MS.gene78400:intron 50.0%
CCAGTTCGGAATTGTGACTC+TGG + chr2.3:5362818-5362837 None:intergenic 50.0%
CCCTTTAACCACGTTAGCGT+AGG + chr2.3:5363079-5363098 None:intergenic 50.0%
CTCCCTTGGTTACAACTCAC+CGG + chr2.3:5362997-5363016 None:intergenic 50.0%
GGATTCAACTCTGTGGTCAC+CGG - chr2.3:5362975-5362994 MS.gene78400:intron 50.0%
GGTGACCACAGAGTTGAATC+CGG + chr2.3:5362976-5362995 None:intergenic 50.0%
GTAACCAAGGGAGCTCAGAA+GGG - chr2.3:5363004-5363023 MS.gene78400:intron 50.0%
GTCCTCGTCCTCATCTAACA+AGG - chr2.3:5363319-5363338 MS.gene78400:intron 50.0%
GTCTTCCAACAAGGTGTAGC+TGG + chr2.3:5363051-5363070 None:intergenic 50.0%
GTGATGAAATCCTCGTCCGT+TGG + chr2.3:5362717-5362736 None:intergenic 50.0%
GTTCACCGGATTCAACTCTG+TGG - chr2.3:5362968-5362987 MS.gene78400:intron 50.0%
TCACCGGTGAGTTGTAACCA+AGG - chr2.3:5362991-5363010 MS.gene78400:intron 50.0%
TGAGCTTAGCTCAGCTGGTT+GGG + chr2.3:5364220-5364239 None:intergenic 50.0%
TGTAACCAAGGGAGCTCAGA+AGG - chr2.3:5363003-5363022 MS.gene78400:intron 50.0%
TTCCAACAAGGTGTAGCTGG+CGG + chr2.3:5363048-5363067 None:intergenic 50.0%
TTCGGAATTGTGACTCTGGG+AGG + chr2.3:5362814-5362833 None:intergenic 50.0%
!!! ATGCAGGCCGTTTTTCTTGG+TGG - chr2.3:5363375-5363394 MS.gene78400:intron 50.0%
!!! TGCAGGCCGTTTTTCTTGGT+GGG - chr2.3:5363376-5363395 MS.gene78400:intron 50.0%
AGAATGGTACCACGGAGTGG+AGG + chr2.3:5364864-5364883 None:intergenic 55.0%
ATCCTCGTCCGTTGGAACCA+TGG + chr2.3:5362709-5362728 None:intergenic 55.0%
ATGACCCTTCTGAGCTCCCT+TGG + chr2.3:5363011-5363030 None:intergenic 55.0%
CTGCATCATTCCTCCACCAC+TGG + chr2.3:5363361-5363380 None:intergenic 55.0%
GAATGGTACCACGGAGTGGA+GGG + chr2.3:5364863-5364882 None:intergenic 55.0%
GCATGTGATCGGATGGTGAG+TGG - chr2.3:5364455-5364474 MS.gene78400:intron 55.0%
GGAGGGACGCCAACAACTTT+GGG + chr2.3:5364846-5364865 None:intergenic 55.0%
GTGAGCTTAGCTCAGCTGGT+TGG + chr2.3:5364221-5364240 None:intergenic 55.0%
GTGGGTCCGGTTCAAGAAGT+GGG - chr2.3:5363394-5363413 MS.gene78400:intron 55.0%
TCCGGTTCAAGAAGTGGGAC+AGG - chr2.3:5363399-5363418 MS.gene78400:intron 55.0%
TGGAGGGACGCCAACAACTT+TGG + chr2.3:5364847-5364866 None:intergenic 55.0%
! CAGTGGTGGAGGAATGATGC+AGG - chr2.3:5363359-5363378 MS.gene78400:intron 55.0%
! GGTGTTTTCTTGCCTCGTGC+TGG - chr2.3:5363420-5363439 MS.gene78400:intron 55.0%
!! GGAGCAGGCCTTGTTAGATG+AGG + chr2.3:5363330-5363349 None:intergenic 55.0%
!! GGCCTTGTTAGATGAGGACG+AGG + chr2.3:5363324-5363343 None:intergenic 55.0%
!!! GCCGTTTTTCTTGGTGGGTC+CGG - chr2.3:5363381-5363400 MS.gene78400:intron 55.0%
CACCATGGTTCCAACGGACG+AGG - chr2.3:5362704-5362723 MS.gene78400:CDS 60.0%
CCAGCTGAGCTAAGCTCACG+AGG - chr2.3:5364222-5364241 MS.gene78400:intron 60.0%
CCATTGCAGACCAGTGGTGG+AGG - chr2.3:5363348-5363367 MS.gene78400:intron 60.0%
CCTCGTGAGCTTAGCTCAGC+TGG + chr2.3:5364225-5364244 None:intergenic 60.0%
CCTGCTCCATTGCAGACCAG+TGG - chr2.3:5363342-5363361 MS.gene78400:intron 60.0%
CTCCGCCAGCTACACCTTGT+TGG - chr2.3:5363043-5363062 MS.gene78400:intron 60.0%
GCAACAACGGTTCCAGCACG+AGG + chr2.3:5363435-5363454 None:intergenic 60.0%
GCTCCATTGCAGACCAGTGG+TGG - chr2.3:5363345-5363364 MS.gene78400:intron 60.0%
GGTGGGTCCGGTTCAAGAAG+TGG - chr2.3:5363393-5363412 MS.gene78400:intron 60.0%
! CCACTGGTCTGCAATGGAGC+AGG + chr2.3:5363345-5363364 None:intergenic 60.0%
! CCTCCACCACTGGTCTGCAA+TGG + chr2.3:5363351-5363370 None:intergenic 60.0%
GTTGGCGTCCCTCCACTCCG+TGG - chr2.3:5364852-5364871 MS.gene78400:intron 70.0%


Chromosome Type Strat End Strand Name
chr2.3 gene 5362648 5366566 5362648 ID=MS.gene78400
chr2.3 mRNA 5362648 5366566 5362648 ID=MS.gene78400.t1;Parent=MS.gene78400
chr2.3 exon 5366378 5366566 5366378 ID=MS.gene78400.t1.exon1;Parent=MS.gene78400.t1
chr2.3 CDS 5366378 5366566 5366378 ID=cds.MS.gene78400.t1;Parent=MS.gene78400.t1
chr2.3 exon 5365734 5366262 5365734 ID=MS.gene78400.t1.exon2;Parent=MS.gene78400.t1
chr2.3 CDS 5365734 5366262 5365734 ID=cds.MS.gene78400.t1;Parent=MS.gene78400.t1
chr2.3 exon 5364341 5364445 5364341 ID=MS.gene78400.t1.exon3;Parent=MS.gene78400.t1
chr2.3 CDS 5364341 5364445 5364341 ID=cds.MS.gene78400.t1;Parent=MS.gene78400.t1
chr2.3 exon 5362648 5362760 5362648 ID=MS.gene78400.t1.exon4;Parent=MS.gene78400.t1
chr2.3 CDS 5362648 5362760 5362648 ID=cds.MS.gene78400.t1;Parent=MS.gene78400.t1
Gene Sequence

>MS.gene78400

ATGGCTGATGACAATAATATGTACCATGATGTTCTCTCAGTTAGCAACAACAATCACACCATGGTTCCAACGGACGAGGATTTCATCACCGAGTTAACTCATCAAATGTCTCAATTCATGCTTCAAGAAGATATTGACGACTCGTTCGATTTCTCATCTATTGCCTCCCAGAGTCACAATTCCGAACTGGTACCATATGTTTCCCTTACTATCTACGCGCTTCCTTTTCAGGTTTATTGAATAACTGATGTATCTGACCTATATTATTAACTCTTCTTTGTTTTCTTTCTTCCTTTTCATGTAGTCGTGGGATTTAATTAGTTCACCGGATTCAACTCTGTGGTCACCGGTGAGTTGTAACCAAGGGAGCTCAGAAGGGTCATCACAGAACTCATCTCCGCCAGCTACACCTTGTTGGAAGACAAAAACCTACGCTAACGTGGTTAAAGGGGTTGAGAAAAATACTAAGAAGCTTAAAGAAACAGCGACTTCTAAACAGAATCATGGTTCTGATATTCAAAGTTTCAAGTCCTCCAATATTGATAGATTTTCATCACACAAAGCATTGATTGAGGAACAAATTAAAGCCATTGAATTAAGGAAAAAACATTACATCAAGCAGCAACAACATGCTCTGTCTCAGAAGCAAGGTAATGGAGGGAACTGTACTCGTCCTCGTCCTCATCTAACAAGGCCTGCTCCATTGCAGACCAGTGGTGGAGGAATGATGCAGGCCGTTTTTCTTGGTGGGTCCGGTTCAAGAAGTGGGACAGGTGTTTTCTTGCCTCGTGCTGGAACCGTTGTTGCTCCATCAGAATCAACCAAAAAGAAAGGTAACGTGGATTATGTTATATCTATTCTGTTCATGTTTTTCACTAGTCGGTAATGGTTGGTTGCGGAATCAAATGGAATGTCTAGTTTCCCACTTTTAATATTCGAATCTGTTTATGATATTTTGTCTCTAAAATTCCACTCTGTTTCTACTTTCTAAGATTTCTTCTTTGTTTGTTTCAACCCAATAAATAATTTTAAAGATATTTACATGGTTTTTAGTTAAGATTTATGAGCAATCAATCATTTCATTTTAAATGTGTGTGATGTGCTGTTATTATTATCCTCAAGTGTCTTCTTTATTAGTTATTGTTAGTTATTGTGATCACTAAATATGCTTCTTGTGTAATTTAACTTAATCCTTAAATGTATTTTTTTTTTTTATCAAGTTAGTTCAAAAGAAACACTAGAAATATGTTTGCAATTTTAATAGATTTAAAATGGATCTCCCATTTGTAACTCTACAAGCTTAGTCCTCCAATTTTAAAAACATCAAATATTTTATCCACCTATATGCTATTGAGGGTGCTTTTTCTACCATGTGATAGGTGAAATCCTAAAGTGGCAACTAGATTGATGAGATGAAAATGAGTTATCTAACTAGATTGATGCTATTAAGGGTGCATTAAATGTAGTAACTCTTACGATATAAGTTAAATGTATATTTTGAGAAAATGAAAGCTTTTTTTTTGTGGTGGTCGGGGTTTGAACCCCGATCTTGCATATCTTATGCACTGTCCCAACCAGCTGAGCTAAGCTCACGAGGACATAGAAAATGAAAATTCAAGTGATAATCCATCTAAATATGTCATGTCATCCACTAAGTTGTCACATAAACATGTAGATTTTTTTTTTAAAAAAAATATTCTTAATATTACGCTTCATTAATATCCACTAATCATCCACATTTAGCCTATCTAGCTAGCTCGTTCCTTAAAAAAAAAAAAACCTATCTATCTTGTCTTTGTACTAGTTTGCATGTGATCGGATGGTGAGTGGTGACCAAAAATATTCTTTATGGTGGTTGAACTTGATTTTTCCCTAATCTATAGTAACATGCTGCTGACATTTGAGTTGATGATTTGTTCCTTTCGGTTCAGTTTTATTTTTCATTTAGCAAGAAACATCAAAAAATTAAGCAGTTAGAAAGTTTGGTGAGCAAAATTGAAGATTCGTTAATGTATGAGTGAATCCTTAATTAATTATATTTAACTGCCAAAACAATATCAAATTCGTTTCACATCTTACATCAAAATTGATTTGTTTTATGCACGTTATATATATTGTTAGGGAAAGGTTGCTCCACTGTCCTTATACCAGCAAGAGTTGTGCAGGCTTTGCAACTACACTTTGACAAAACGGCTCCCAAAGTTGTTGGCGTCCCTCCACTCCGTGGTACCATTCTTAATTTATTATTGTTAATTATACCATCAAGCAACAACTTTGATTTGTTGTTTATTTATTGCTATTCTTCATGCCGTTTAGTTACTACTCTCCTAATAAATAAATAATCTTACCCAACAAAATATGTGGATGAGATGATAAGAATCTCTTCCAAGTTAACTAAAAGTCTTAGGTTCGAATCAAACTCTAAAAATACAACAATGTTAAATCTTTTTAAGGAGAATTTTTTTACCATTGTAATCCTAATCTATTCGAGATGTTAGTTTGTGCAATTATTCGTATAGGACACCCGATTTAAATTAAAAAAAAAAAAACAAAACTCATGACCATGAATTTGTTGCTTCATCCTTAGCAACATGACATTCAATAAACTTTTAGTTGTTATATCAATTCCCATTTTATATGCATTTAAAATTTATTTGGGTAATTCCTAAGTTTAATGCTCTAAGTTTGGTTGTATTCAATATAGTCTATCAATTTAAATGATTATCCTTTCTTTTTAAAATTATTTGGGCAATTCCTAAGTTTTATACTCTAAGTTTTGTAGCACCAAAAATAAAATACTCTTAAGTTTTGTAGAAAACAAAAAAACTCTAAGTTTGCTACTGACTTTCCTATTTCATTTTCTTATCAATCATATAAATTTATAAACAATTTCTACATGGAAAATTCTACGGTGAAGTCATGAGAATGACTATTTTGGTGAAGTTAACACTATAACATCAAAAATGTAATGAGTAACACCTCACTGTTTGTACAATGGCATCAAAAGTTCAGTCCTCATAGTATCATCAATTCATCAGATTAACAAGATTACACTTAATCTAATTTTATCCTACCTTGTTATAAGCGTAACACCCCACAAAGTGTAACACTTAAGACTATCACATAATATCATCAACTCTGCTTGCATGTTAAAGATTCCGACAATAAAATAGTACCTGTAAGTCCAAGATATTGCAAGCATTACTAGATGGATTACTATTGATCATTAATCAGTAATCAACAATTTAAAAATGAATCAAACATTAAGAACAAAATTAACTTTTTCAAAAAAGTCAGTTGTCTGACTGCACTATAGAAGCTCCCTTTCTACATTTGTTTAATTTATTCACTCTTTGATTTCCTATCAATTTCTCATCTGTTTGCTTACACTTAAGTCTTGCGCAATGTTAGATTGTATTAAATGTCACACTCACACACACATCATCTAGTTGTTTTTTTATACTTTTCTTTAGAAAAATTGGCATACGTCATGTTTAAAAAAAACAAAAATTATGAATAAATTAAAATTAGTATATTCTATGAAAGACTTCTAGTTAGTACTAATCTCTCTATGATTACTTTGATTTGCAATTCTTAATAATGGAATGTATTAGCTGTCCAATTTTATTTTTCGAGACTGCTGCAATTTATTCCAACAGTCCAAATACTCGGTATCAACTAGCAATTTGTCGCCAACTAGTTCTATAATATAAAAAAAACACTGTCTGATTTGATTTTCATCTATTTTTTATTTATTTGTTGTTAATCAATTTGCTCTATAATATTGTTATTATTATGAATTGCAGATCTTGTTTTGAACCATAGGGAAGGTATGTTTTCACTTGAGAATCACCAGTCAACAAAAGCAGCAACAGATGTCCAGAATGATATGATTCTGCCTCAAGAATGGACATATTGA
Protein sequence

>MS.gene78400.t1

MADDNNMYHDVLSVSNNNHTMVPTDEDFITELTHQMSQFMLQEDIDDSFDFSSIASQSHNSELSWDLISSPDSTLWSPVSCNQGSSEGSSQNSSPPATPCWKTKTYANVVKGVEKNTKKLKETATSKQNHGSDIQSFKSSNIDRFSSHKALIEEQIKAIELRKKHYIKQQQHALSQKQGNGGNCTRPRPHLTRPAPLQTSGGGMMQAVFLGGSGSRSGTGVFLPRAGTVVAPSESTKKKGKGCSTVLIPARVVQALQLHFDKTAPKVVGVPPLRDLVLNHREGMFSLENHQSTKAATDVQNDMILPQEWTY