AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene78471

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene78471.t1 MTR_2g097210 96.501 343 11 1 1 343 1 342 0.0 677
MS.gene78471.t1 MTR_2g097210 96.346 301 10 1 1 301 1 300 0.0 586
MS.gene78471.t1 MTR_4g021755 64.706 306 105 2 37 342 34 336 1.09e-150 430
MS.gene78471.t1 MTR_7g498280 59.412 340 122 3 1 338 1 326 4.60e-128 370
MS.gene78471.t1 MTR_7g061780 61.311 305 112 3 23 326 4 303 2.86e-125 363
MS.gene78471.t1 MTR_1g101310 53.191 329 135 3 16 339 5 319 4.79e-114 333
MS.gene78471.t1 MTR_2g103600 55.636 275 114 3 44 312 30 302 2.18e-99 296
MS.gene78471.t1 MTR_3g073530 40.533 338 188 5 13 343 9 340 1.37e-79 248
MS.gene78471.t1 MTR_2g088290 36.462 277 116 7 65 340 1 218 7.73e-43 148
MS.gene78471.t1 MTR_2g088315 49.398 83 42 0 108 190 1 83 2.07e-20 84.7
MS.gene78471.t1 MTR_8g106030 37.931 145 73 3 177 320 99 227 9.23e-19 84.3
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene78471.t1 AT2G29050 63.905 338 119 2 6 343 15 349 3.31e-157 446
MS.gene78471.t1 AT3G53780 62.662 308 107 2 35 342 54 353 1.59e-137 396
MS.gene78471.t1 AT1G63120 63.946 294 98 2 44 335 30 317 2.93e-129 372
MS.gene78471.t1 AT2G29050 58.104 327 106 3 6 304 15 338 2.78e-126 366
MS.gene78471.t1 AT5G07250 60.061 328 116 3 21 337 22 345 2.00e-125 363
MS.gene78471.t1 AT1G12750 55.882 306 130 2 32 335 5 307 8.77e-123 355
MS.gene78471.t1 AT1G12750 55.882 306 130 2 32 335 5 307 8.77e-123 355
MS.gene78471.t1 AT1G12750 55.882 306 130 2 32 335 5 307 8.77e-123 355
MS.gene78471.t1 AT1G12750 55.882 306 130 2 32 335 5 307 8.77e-123 355
MS.gene78471.t1 AT1G12750 55.882 306 130 2 32 335 5 307 8.77e-123 355
MS.gene78471.t1 AT3G53780 62.547 267 92 2 35 301 54 312 1.29e-114 335
MS.gene78471.t1 AT3G53780 63.559 236 80 1 108 343 1 230 9.98e-108 316
MS.gene78471.t1 AT1G52580 54.545 297 126 6 20 311 4 296 6.52e-98 292
MS.gene78471.t1 AT4G23070 55.326 291 125 2 44 334 28 313 2.37e-94 283
MS.gene78471.t1 AT5G07250 49.695 328 103 4 21 337 22 298 4.31e-89 270
MS.gene78471.t1 AT1G77860 45.151 299 159 4 48 343 49 345 3.77e-85 261
MS.gene78471.t1 AT1G77860 45.151 299 159 4 48 343 49 345 8.09e-85 261
MS.gene78471.t1 AT1G12750 60.000 185 72 1 32 214 5 189 6.47e-77 235
MS.gene78471.t1 AT1G12750 59.574 188 74 1 32 217 5 192 9.39e-77 234

Find 83 sgRNAs with CRISPR-Local

Find 163 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
ATTCGGCTTGAACAAGAATT+TGG 0.175005 2.2:+5072290 MS.gene78471:CDS
TGATTCGTCCTCAGTTTAAA+TGG 0.238851 2.2:+5072935 MS.gene78471:CDS
CTCTATGTGATATCTGGTTT+TGG 0.246364 2.2:+5072538 MS.gene78471:CDS
GGATTTCTCTATGTGATATC+TGG 0.262897 2.2:+5072532 MS.gene78471:CDS
TCTCAGAGCTCTTCATTAAT+TGG 0.278075 2.2:+5072647 MS.gene78471:CDS
TCTTTCCTATTACTCATTGC+AGG 0.286895 2.2:+5073048 MS.gene78471:CDS
CTTACCCTGCAATGAGTAAT+AGG 0.292148 2.2:-5073053 None:intergenic
TTCGCTCATCTAGGAGGATT+TGG 0.297054 2.2:+5072883 MS.gene78471:CDS
GGCGCCTTGTCTCTTGTATA+TGG 0.304879 2.2:+5072210 MS.gene78471:CDS
CTTGAACAAGAATTTGGATT+TGG 0.310501 2.2:+5072296 MS.gene78471:CDS
CATCTAGGAGGATTTGGTTC+TGG 0.319719 2.2:+5072889 MS.gene78471:CDS
TCACTCGTGATCGTGATTTC+AGG 0.355861 2.2:+5071070 MS.gene78471:CDS
CTTAAAGAAAACCCTCTCTT+TGG 0.366436 2.2:+5071246 MS.gene78471:CDS
TGGCACCACCGTTGAGGTTA+AGG 0.367443 2.2:+5070999 MS.gene78471:CDS
GGCATCTCAGTTGGTGCATC+TGG 0.372637 2.2:+5072595 MS.gene78471:CDS
TTTGGTTCTGGCTTTCTGCT+TGG 0.373387 2.2:+5072901 MS.gene78471:CDS
CTTGTATATGGTTGCATGGT+GGG 0.379600 2.2:+5072222 MS.gene78471:CDS
TGTCCTTTCGCAGTTAGAAT+TGG 0.379685 2.2:+5072511 MS.gene78471:intron
TTGGACGATATATGCCAATA+AGG 0.382911 2.2:+5072666 MS.gene78471:CDS
GGTGCATCTGGTGCATTGTT+TGG 0.382955 2.2:+5072607 MS.gene78471:CDS
AGCCAACAACAGTCTAAATC+TGG 0.385356 2.2:+5072961 MS.gene78471:CDS
ACCTTATCAATATGTGTTGT+GGG 0.394616 2.2:+5073020 MS.gene78471:CDS
ATCCAGATTTAGACTGTTGT+TGG 0.400472 2.2:-5072963 None:intergenic
GTTGTGTTCCTACCTCAAAA+TGG 0.409054 2.2:+5073300 MS.gene78471:CDS
GTTGTAATAAATTTAGCTGT+TGG 0.441288 2.2:+5072838 MS.gene78471:CDS
GTCTGCGTTATTCATACAAA+CGG 0.445629 2.2:+5072573 MS.gene78471:CDS
TGTCCTGAGAATGCATTTCA+TGG 0.455723 2.2:+5071180 MS.gene78471:CDS
TACCTTATCAATATGTGTTG+TGG 0.455869 2.2:+5073019 MS.gene78471:CDS
GGTGCATTGTTTGGCTTACT+CGG 0.462230 2.2:+5072616 MS.gene78471:CDS
TTTCAGGCTCTTTAAGCAAT+GGG 0.470163 2.2:+5071086 MS.gene78471:CDS
GTTGACAATTTCGCTCATCT+AGG 0.472592 2.2:+5072874 MS.gene78471:CDS
GTCTCTTGTATATGGTTGCA+TGG 0.482616 2.2:+5072218 MS.gene78471:CDS
AAGGAGAATCTTCCAAGAAA+AGG 0.495328 2.2:-5071216 None:intergenic
TCTTTAAGCAATGGGTTCCA+TGG 0.504864 2.2:+5071094 MS.gene78471:CDS
ACCCACAACACATATTGATA+AGG 0.507027 2.2:-5073021 None:intergenic
TACCTCAAAATGGAGTTGTC+AGG 0.507679 2.2:+5073310 MS.gene78471:CDS
GGGATGGTTCTACTCTTTCG+AGG 0.511587 2.2:+5073236 MS.gene78471:CDS
ATTTCAGGCTCTTTAAGCAA+TGG 0.516293 2.2:+5071085 MS.gene78471:CDS
CATTGTTTGGCTTACTCGGA+GGG 0.524954 2.2:+5072620 MS.gene78471:CDS
ACGTTGGCAAACACAAAAGT+TGG 0.526318 2.2:-5071123 None:intergenic
CTTTCCTATTACTCATTGCA+GGG 0.535119 2.2:+5073049 MS.gene78471:CDS
GTACCATGAAATGCATTCTC+AGG 0.536653 2.2:-5071183 None:intergenic
TCAACATGTTGGCAAGTACA+TGG 0.537008 2.2:-5072250 None:intergenic
AAATCACGATCACGAGTGAC+GGG 0.541550 2.2:-5071066 None:intergenic
TGACAGATTCATTACTGGGA+TGG 0.543513 2.2:+5073220 MS.gene78471:intron
TAAACTGAGGACGAATCAAA+AGG 0.544513 2.2:-5072930 None:intergenic
TGCTCTAGAAGTAGATAAAG+TGG 0.545530 2.2:+5072169 MS.gene78471:CDS
AAAACTCACGTAAAGGAAGA+AGG 0.547703 2.2:-5071270 None:intergenic
GACAATTTCGCTCATCTAGG+AGG 0.549429 2.2:+5072877 MS.gene78471:CDS
TCTGCGTTATTCATACAAAC+GGG 0.553724 2.2:+5072574 MS.gene78471:CDS
ACAAAAGTTGGAACAAGCCA+TGG 0.556908 2.2:-5071111 None:intergenic
ATACAAACGGGCATCTCAGT+TGG 0.569101 2.2:+5072586 MS.gene78471:CDS
GAAATCACGATCACGAGTGA+CGG 0.572889 2.2:-5071067 None:intergenic
ACGACGAAGACGGAGACCCA+CGG 0.573842 2.2:-5071032 None:intergenic
TCAACGGTGGTGCCACCATG+TGG 0.575383 2.2:-5070991 None:intergenic
AAATCATAATCATGAGTGGA+GGG 0.582372 2.2:+5070962 None:intergenic
TTGTATATGGTTGCATGGTG+GGG 0.585338 2.2:+5072223 MS.gene78471:CDS
AGGAAGAAGGACCAAAGAGA+GGG 0.589526 2.2:-5071257 None:intergenic
TGGTTCATGAACACGAAGGT+TGG 0.597343 2.2:+5072189 MS.gene78471:CDS
AACAATGAAAATAATAACGT+TGG 0.597447 2.2:-5071139 None:intergenic
GGAACAAAGCATTTATTGTG+AGG 0.597670 2.2:+5073331 MS.gene78471:CDS
TTAAGGTTCATCCTCGCCGT+GGG 0.597978 2.2:+5071016 MS.gene78471:CDS
TCTTGTATATGGTTGCATGG+TGG 0.604832 2.2:+5072221 MS.gene78471:CDS
GCATTGTTTGGCTTACTCGG+AGG 0.606501 2.2:+5072619 MS.gene78471:CDS
GTTAAGGTTCATCCTCGCCG+TGG 0.606777 2.2:+5071015 MS.gene78471:CDS
CGAAATTGTCAACATGTGGA+AGG 0.611940 2.2:-5072864 None:intergenic
TGAGCGAAATTGTCAACATG+TGG 0.615330 2.2:-5072868 None:intergenic
GGATTCGACGACGACGAAGA+CGG 0.615599 2.2:-5071042 None:intergenic
CAACGGTGGTGCCACCATGT+GGG 0.618108 2.2:-5070990 None:intergenic
ACATGGTGGCACCACCGTTG+AGG 0.621441 2.2:+5070993 MS.gene78471:CDS
AAGGAAGAAGGACCAAAGAG+AGG 0.633246 2.2:-5071258 None:intergenic
TGGCTTATCCATTTAAACTG+AGG 0.633333 2.2:-5072943 None:intergenic
AAAATCATAATCATGAGTGG+AGG 0.633390 2.2:+5070961 None:intergenic
TCACGATCACGAGTGACGGG+AGG 0.634822 2.2:-5071063 None:intergenic
ACTTGAATGAACGTTGCTCG+TGG 0.663668 2.2:+5073264 MS.gene78471:CDS
AGGATGAACCTTAACCTCAA+CGG 0.671763 2.2:-5071007 None:intergenic
TCATAATCATGAGTGGAGGG+AGG 0.674267 2.2:+5070965 None:intergenic
GAAGACGGAGACCCACGGCG+AGG 0.680052 2.2:-5071027 None:intergenic
AGTGGAGGGAGGAACCCACA+TGG 0.690830 2.2:+5070976 MS.gene78471:CDS
GCAACCATATACAAGAGACA+AGG 0.709780 2.2:-5072214 None:intergenic
ATGAACCTTAACCTCAACGG+TGG 0.726735 2.2:-5071004 None:intergenic
GGAGGGAGGAACCCACATGG+TGG 0.736064 2.2:+5070979 MS.gene78471:CDS
AAAGTGGTTCATGAACACGA+AGG 0.793505 2.2:+5072185 MS.gene78471:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AAGACAATAAATTATTTATC+AGG - chr2.2:5072730-5072749 None:intergenic 15.0%
!! AATGATATAAATACAGATAA+TGG - chr2.2:5072389-5072408 None:intergenic 15.0%
!! ATATTACCTTTAATATTCTT+AGG + chr2.2:5073104-5073123 MS.gene78471:intron 15.0%
!! TAAATAATTTATTGTCTTTG+CGG + chr2.2:5072732-5072751 MS.gene78471:intron 15.0%
!! AACAATGAAAATAATAACGT+TGG - chr2.2:5071142-5071161 None:intergenic 20.0%
!! ATGAACATGTGTTTATAAAT+GGG + chr2.2:5071718-5071737 MS.gene78471:intron 20.0%
!! TACATTAATCAAGTTATGAT+TGG + chr2.2:5071375-5071394 MS.gene78471:intron 20.0%
!!! AGTAGTATTTAATCTAAAGT+TGG + chr2.2:5073190-5073209 MS.gene78471:intron 20.0%
!!! TTGTTTTTACATAAATGTTC+TGG + chr2.2:5072407-5072426 MS.gene78471:intron 20.0%
! CAAAATGGAAAGAAATAGAA+TGG - chr2.2:5072770-5072789 None:intergenic 25.0%
! CATGAACATGTGTTTATAAA+TGG + chr2.2:5071717-5071736 MS.gene78471:intron 25.0%
! GCTATTGCAAATACATAATT+TGG - chr2.2:5072040-5072059 None:intergenic 25.0%
! GTTGTAATAAATTTAGCTGT+TGG + chr2.2:5072838-5072857 MS.gene78471:CDS 25.0%
! TATCTGCCTAAAATTAAGAT+TGG - chr2.2:5072075-5072094 None:intergenic 25.0%
! TATGTATTTGCAATAGCTAA+TGG + chr2.2:5072043-5072062 MS.gene78471:intron 25.0%
! TGAACATGTGTTTATAAATG+GGG + chr2.2:5071719-5071738 MS.gene78471:intron 25.0%
! TTAATCAAGTTATGATTGGT+TGG + chr2.2:5071379-5071398 MS.gene78471:intron 25.0%
! TTCATAGATTACATTCTCTA+TGG + chr2.2:5071960-5071979 MS.gene78471:intron 25.0%
!! ACAGTACCAATCTTAATTTT+AGG + chr2.2:5072066-5072085 MS.gene78471:intron 25.0%
!! ATGTTGAGTCTTCTTTTTAT+TGG + chr2.2:5072266-5072285 MS.gene78471:CDS 25.0%
!! GTCTTCTTTTTATTGGAATT+CGG + chr2.2:5072273-5072292 MS.gene78471:CDS 25.0%
!!! AATTTTGACAGATTCATTAC+TGG + chr2.2:5073215-5073234 MS.gene78471:intron 25.0%
!!! ATTTTGACAGATTCATTACT+GGG + chr2.2:5073216-5073235 MS.gene78471:intron 25.0%
!!! TTTTAGTTCATTCACCTTAT+TGG - chr2.2:5072683-5072702 None:intergenic 25.0%
AAAAACCTAATAGATGTGTG+AGG + chr2.2:5071901-5071920 MS.gene78471:intron 30.0%
AAAAAGAAGACTCAACATGT+TGG - chr2.2:5072264-5072283 None:intergenic 30.0%
AAAACCTAATAGATGTGTGA+GGG + chr2.2:5071902-5071921 MS.gene78471:intron 30.0%
AACAAAAACGTGTGTTTGTA+AGG + chr2.2:5071860-5071879 MS.gene78471:intron 30.0%
AACATGTGTTTATAAATGGG+GGG + chr2.2:5071721-5071740 MS.gene78471:intron 30.0%
ACCTTATCAATATGTGTTGT+GGG + chr2.2:5073020-5073039 MS.gene78471:CDS 30.0%
CAAAACATCATGAGACAAAA+TGG - chr2.2:5072785-5072804 None:intergenic 30.0%
CCAAGTCCTAAGAATATTAA+AGG - chr2.2:5073113-5073132 None:intergenic 30.0%
CCTTTAATATTCTTAGGACT+TGG + chr2.2:5073110-5073129 MS.gene78471:intron 30.0%
CTTGAACAAGAATTTGGATT+TGG + chr2.2:5072296-5072315 MS.gene78471:CDS 30.0%
GAAAAAGAAAACTCACGTAA+AGG - chr2.2:5071280-5071299 None:intergenic 30.0%
GAACATGTGTTTATAAATGG+GGG + chr2.2:5071720-5071739 MS.gene78471:intron 30.0%
GAATGTAATCTATGAATCAC+CGG - chr2.2:5071956-5071975 None:intergenic 30.0%
GGAAAGAAATAGAATGGTTT+AGG - chr2.2:5072764-5072783 None:intergenic 30.0%
TACCTTATCAATATGTGTTG+TGG + chr2.2:5073019-5073038 MS.gene78471:CDS 30.0%
TGAGAATGAGTTTATTAGTG+TGG + chr2.2:5071500-5071519 MS.gene78471:intron 30.0%
TTAATATTTACTCCTTCGTC+CGG - chr2.2:5071806-5071825 None:intergenic 30.0%
! ACAAGAATTTGGATTTGGTA+TGG + chr2.2:5072301-5072320 MS.gene78471:intron 30.0%
! ACACTTTAGATGTCTAAGAT+TGG + chr2.2:5071653-5071672 MS.gene78471:intron 30.0%
! ACTTTAGATGTCTAAGATTG+GGG + chr2.2:5071655-5071674 MS.gene78471:intron 30.0%
! ATTTTCAGATTACAGGAGAT+GGG + chr2.2:5072146-5072165 MS.gene78471:intron 30.0%
! CACTTTAGATGTCTAAGATT+GGG + chr2.2:5071654-5071673 MS.gene78471:intron 30.0%
! GTTTTCTTTAAGTGGTTGAA+AGG - chr2.2:5071238-5071257 None:intergenic 30.0%
! TTTTAGGCAGATATATCTCT+TGG + chr2.2:5072082-5072101 MS.gene78471:intron 30.0%
!! AGTTAAATTGATTCGAGCTA+AGG + chr2.2:5071750-5071769 MS.gene78471:intron 30.0%
!! GTTAAATTGATTCGAGCTAA+GGG + chr2.2:5071751-5071770 MS.gene78471:intron 30.0%
!! TACAGATAATGGAATACTGT+TGG - chr2.2:5072378-5072397 None:intergenic 30.0%
AAAACTCACGTAAAGGAAGA+AGG - chr2.2:5071273-5071292 None:intergenic 35.0%
AAGGAGAATCTTCCAAGAAA+AGG - chr2.2:5071219-5071238 None:intergenic 35.0%
AATCCAATTCTAACTGCGAA+AGG - chr2.2:5072517-5072536 None:intergenic 35.0%
ACCCACAACACATATTGATA+AGG - chr2.2:5073024-5073043 None:intergenic 35.0%
ATCCAGATTTAGACTGTTGT+TGG - chr2.2:5072966-5072985 None:intergenic 35.0%
ATTTCAGGCTCTTTAAGCAA+TGG + chr2.2:5071085-5071104 MS.gene78471:CDS 35.0%
CGATATACTTGAACAATGCA+AGG - chr2.2:5072340-5072359 None:intergenic 35.0%
CTTAAAGAAAACCCTCTCTT+TGG + chr2.2:5071246-5071265 MS.gene78471:CDS 35.0%
CTTTCCTATTACTCATTGCA+GGG + chr2.2:5073049-5073068 MS.gene78471:CDS 35.0%
GGATTTCTCTATGTGATATC+TGG + chr2.2:5072532-5072551 MS.gene78471:CDS 35.0%
GTCTGCGTTATTCATACAAA+CGG + chr2.2:5072573-5072592 MS.gene78471:CDS 35.0%
TAAACTGAGGACGAATCAAA+AGG - chr2.2:5072933-5072952 None:intergenic 35.0%
TAATATTTACTCCTTCGTCC+GGG - chr2.2:5071805-5071824 None:intergenic 35.0%
TCTCAGAGCTCTTCATTAAT+TGG + chr2.2:5072647-5072666 MS.gene78471:CDS 35.0%
TCTGCGTTATTCATACAAAC+GGG + chr2.2:5072574-5072593 MS.gene78471:CDS 35.0%
TCTTTCCTATTACTCATTGC+AGG + chr2.2:5073048-5073067 MS.gene78471:CDS 35.0%
TGATTCGTCCTCAGTTTAAA+TGG + chr2.2:5072935-5072954 MS.gene78471:CDS 35.0%
TGGCTTATCCATTTAAACTG+AGG - chr2.2:5072946-5072965 None:intergenic 35.0%
TTGGACGATATATGCCAATA+AGG + chr2.2:5072666-5072685 MS.gene78471:CDS 35.0%
TTTCAGGCTCTTTAAGCAAT+GGG + chr2.2:5071086-5071105 MS.gene78471:CDS 35.0%
! AAGAGAGGGTTTTCTTTAAG+TGG - chr2.2:5071246-5071265 None:intergenic 35.0%
! ATTCGGCTTGAACAAGAATT+TGG + chr2.2:5072290-5072309 MS.gene78471:CDS 35.0%
! ATTTGGATTTGGTATGGTTC+TGG + chr2.2:5072307-5072326 MS.gene78471:intron 35.0%
! CATTTTCAGATTACAGGAGA+TGG + chr2.2:5072145-5072164 MS.gene78471:intron 35.0%
! CTTTAGATGTCTAAGATTGG+GGG + chr2.2:5071656-5071675 MS.gene78471:intron 35.0%
! TACTCGCATTTTCAGATTAC+AGG + chr2.2:5072139-5072158 MS.gene78471:intron 35.0%
!! CTCTATGTGATATCTGGTTT+TGG + chr2.2:5072538-5072557 MS.gene78471:CDS 35.0%
!! GATAATGGAATACTGTTGGT+CGG - chr2.2:5072374-5072393 None:intergenic 35.0%
!! TGCTCTAGAAGTAGATAAAG+TGG + chr2.2:5072169-5072188 MS.gene78471:CDS 35.0%
!!! ATGTGATATCTGGTTTTGGA+GGG + chr2.2:5072542-5072561 MS.gene78471:CDS 35.0%
!!! TATGTGATATCTGGTTTTGG+AGG + chr2.2:5072541-5072560 MS.gene78471:CDS 35.0%
AAAGTGGTTCATGAACACGA+AGG + chr2.2:5072185-5072204 MS.gene78471:CDS 40.0%
ACAAAAGTTGGAACAAGCCA+TGG - chr2.2:5071114-5071133 None:intergenic 40.0%
ACAACCCTCACACATCTATT+AGG - chr2.2:5071909-5071928 None:intergenic 40.0%
ACGTTGGCAAACACAAAAGT+TGG - chr2.2:5071126-5071145 None:intergenic 40.0%
AGCCAACAACAGTCTAAATC+TGG + chr2.2:5072961-5072980 MS.gene78471:CDS 40.0%
AGGATGAACCTTAACCTCAA+CGG - chr2.2:5071010-5071029 None:intergenic 40.0%
CGAAATTGTCAACATGTGGA+AGG - chr2.2:5072867-5072886 None:intergenic 40.0%
CTTACCCTGCAATGAGTAAT+AGG - chr2.2:5073056-5073075 None:intergenic 40.0%
CTTGTATATGGTTGCATGGT+GGG + chr2.2:5072222-5072241 MS.gene78471:CDS 40.0%
GCAACCATATACAAGAGACA+AGG - chr2.2:5072217-5072236 None:intergenic 40.0%
GCCTAACTCATTCACAAAAC+TGG - chr2.2:5071545-5071564 None:intergenic 40.0%
GTACCATGAAATGCATTCTC+AGG - chr2.2:5071186-5071205 None:intergenic 40.0%
GTCTCTTGTATATGGTTGCA+TGG + chr2.2:5072218-5072237 MS.gene78471:CDS 40.0%
GTGTGTTTGTAAGGATGCAT+AGG + chr2.2:5071869-5071888 MS.gene78471:intron 40.0%
GTGTTTATAAATGGGGGGTT+GGG + chr2.2:5071726-5071745 MS.gene78471:intron 40.0%
GTTGACAATTTCGCTCATCT+AGG + chr2.2:5072874-5072893 MS.gene78471:CDS 40.0%
GTTGTGTTCCTACCTCAAAA+TGG + chr2.2:5073300-5073319 MS.gene78471:CDS 40.0%
TAACTGCGAAAGGACAAGAA+AGG - chr2.2:5072507-5072526 None:intergenic 40.0%
TACCTCAAAATGGAGTTGTC+AGG + chr2.2:5073310-5073329 MS.gene78471:CDS 40.0%
TCAACATGTTGGCAAGTACA+TGG - chr2.2:5072253-5072272 None:intergenic 40.0%
TCTTGTATATGGTTGCATGG+TGG + chr2.2:5072221-5072240 MS.gene78471:CDS 40.0%
TGACAGATTCATTACTGGGA+TGG + chr2.2:5073220-5073239 MS.gene78471:intron 40.0%
TGAGCGAAATTGTCAACATG+TGG - chr2.2:5072871-5072890 None:intergenic 40.0%
TGTCCTGAGAATGCATTTCA+TGG + chr2.2:5071180-5071199 MS.gene78471:CDS 40.0%
TGTCCTTTCGCAGTTAGAAT+TGG + chr2.2:5072511-5072530 MS.gene78471:intron 40.0%
TGTGTTTATAAATGGGGGGT+TGG + chr2.2:5071725-5071744 MS.gene78471:intron 40.0%
TTGTATATGGTTGCATGGTG+GGG + chr2.2:5072223-5072242 MS.gene78471:CDS 40.0%
! ATGTCTAAGATTGGGGGTAA+GGG + chr2.2:5071662-5071681 MS.gene78471:intron 40.0%
! GTCTCATGATGTTTTGCAGA+TGG + chr2.2:5072789-5072808 MS.gene78471:intron 40.0%
! TCTTTAAGCAATGGGTTCCA+TGG + chr2.2:5071094-5071113 MS.gene78471:CDS 40.0%
! TTCCTGACAACTCCATTTTG+AGG - chr2.2:5073315-5073334 None:intergenic 40.0%
!! ACTTGTGTTGCTCCTTTTCT+TGG + chr2.2:5071204-5071223 MS.gene78471:CDS 40.0%
!! GCCAGTTTTGTGAATGAGTT+AGG + chr2.2:5071541-5071560 MS.gene78471:intron 40.0%
!!! TGACAACTCCATTTTGAGGT+AGG - chr2.2:5073311-5073330 None:intergenic 40.0%
AAATCACGATCACGAGTGAC+GGG - chr2.2:5071069-5071088 None:intergenic 45.0%
AAGGAAGAAGGACCAAAGAG+AGG - chr2.2:5071261-5071280 None:intergenic 45.0%
ACTTGAATGAACGTTGCTCG+TGG + chr2.2:5073264-5073283 MS.gene78471:CDS 45.0%
AGGAAGAAGGACCAAAGAGA+GGG - chr2.2:5071260-5071279 None:intergenic 45.0%
ATACAAACGGGCATCTCAGT+TGG + chr2.2:5072586-5072605 MS.gene78471:CDS 45.0%
ATGAACCTTAACCTCAACGG+TGG - chr2.2:5071007-5071026 None:intergenic 45.0%
CGAGCTAAGGGATAAGAAGT+TGG + chr2.2:5071763-5071782 MS.gene78471:intron 45.0%
GAAATCACGATCACGAGTGA+CGG - chr2.2:5071070-5071089 None:intergenic 45.0%
GACAATTTCGCTCATCTAGG+AGG + chr2.2:5072877-5072896 MS.gene78471:CDS 45.0%
GCTAAGGGATAAGAAGTTGG+AGG + chr2.2:5071766-5071785 MS.gene78471:intron 45.0%
TCACTCGTGATCGTGATTTC+AGG + chr2.2:5071070-5071089 MS.gene78471:CDS 45.0%
TCTTGTGTCTGTGTCTCTAC+CGG + chr2.2:5071934-5071953 MS.gene78471:intron 45.0%
TGGTTCATGAACACGAAGGT+TGG + chr2.2:5072189-5072208 MS.gene78471:CDS 45.0%
TTCGCTCATCTAGGAGGATT+TGG + chr2.2:5072883-5072902 MS.gene78471:CDS 45.0%
! CATCTAAAGTGTGACTCGTG+TGG - chr2.2:5071645-5071664 None:intergenic 45.0%
! CATCTAGGAGGATTTGGTTC+TGG + chr2.2:5072889-5072908 MS.gene78471:CDS 45.0%
! CATTGTTTGGCTTACTCGGA+GGG + chr2.2:5072620-5072639 MS.gene78471:CDS 45.0%
! GATGTCTAAGATTGGGGGTA+AGG + chr2.2:5071661-5071680 MS.gene78471:intron 45.0%
! GGTGCATTGTTTGGCTTACT+CGG + chr2.2:5072616-5072635 MS.gene78471:CDS 45.0%
!! GGAGGTTCTAAGTTCAAACC+CGG + chr2.2:5071784-5071803 MS.gene78471:intron 45.0%
!! TTTGGTTCTGGCTTTCTGCT+TGG + chr2.2:5072901-5072920 MS.gene78471:CDS 45.0%
CTAAGTTCAAACCCGGACGA+AGG + chr2.2:5071791-5071810 MS.gene78471:intron 50.0%
GCTATCTGGCCACCATTATC+AGG + chr2.2:5071619-5071638 MS.gene78471:intron 50.0%
GGCGCCTTGTCTCTTGTATA+TGG + chr2.2:5072210-5072229 MS.gene78471:CDS 50.0%
GGTGCATCTGGTGCATTGTT+TGG + chr2.2:5072607-5072626 MS.gene78471:CDS 50.0%
TGACTCGTGTGGCCTGATAA+TGG - chr2.2:5071634-5071653 None:intergenic 50.0%
TTAAGGTTCATCCTCGCCGT+GGG + chr2.2:5071016-5071035 MS.gene78471:CDS 50.0%
! GATAATGGTGGCCAGATAGC+AGG - chr2.2:5071619-5071638 None:intergenic 50.0%
! GCATTGTTTGGCTTACTCGG+AGG + chr2.2:5072619-5072638 MS.gene78471:CDS 50.0%
! GGGATGGTTCTACTCTTTCG+AGG + chr2.2:5073236-5073255 MS.gene78471:CDS 50.0%
AATGGTGGCCAGATAGCAGG+CGG - chr2.2:5071616-5071635 None:intergenic 55.0%
CCAGATAGCAGGCGGTTCAA+TGG - chr2.2:5071608-5071627 None:intergenic 55.0%
CCATTGAACCGCCTGCTATC+TGG + chr2.2:5071605-5071624 MS.gene78471:intron 55.0%
GCAGGCGGTTCAATGGATCT+TGG - chr2.2:5071601-5071620 None:intergenic 55.0%
GGATTCGACGACGACGAAGA+CGG - chr2.2:5071045-5071064 None:intergenic 55.0%
GTTAAGGTTCATCCTCGCCG+TGG + chr2.2:5071015-5071034 MS.gene78471:CDS 55.0%
TGGCACCACCGTTGAGGTTA+AGG + chr2.2:5070999-5071018 MS.gene78471:CDS 55.0%
! GGCATCTCAGTTGGTGCATC+TGG + chr2.2:5072595-5072614 MS.gene78471:CDS 55.0%
!! CTCGTGTGGCCTGATAATGG+TGG - chr2.2:5071631-5071650 None:intergenic 55.0%
ACATGGTGGCACCACCGTTG+AGG + chr2.2:5070993-5071012 MS.gene78471:CDS 60.0%
ACGACGAAGACGGAGACCCA+CGG - chr2.2:5071035-5071054 None:intergenic 60.0%
AGTGGAGGGAGGAACCCACA+TGG + chr2.2:5070976-5070995 MS.gene78471:CDS 60.0%
! TCACGATCACGAGTGACGGG+AGG - chr2.2:5071066-5071085 None:intergenic 60.0%
!! CAACGGTGGTGCCACCATGT+GGG - chr2.2:5070993-5071012 None:intergenic 60.0%
!! TCAACGGTGGTGCCACCATG+TGG - chr2.2:5070994-5071013 None:intergenic 60.0%
GGAGGGAGGAACCCACATGG+TGG + chr2.2:5070979-5070998 MS.gene78471:CDS 65.0%
GAAGACGGAGACCCACGGCG+AGG - chr2.2:5071030-5071049 None:intergenic 70.0%


Chromosome Type Strat End Strand Name
chr2.2 gene 5070973 5073352 5070973 ID=MS.gene78471
chr2.2 mRNA 5070973 5073352 5070973 ID=MS.gene78471.t1;Parent=MS.gene78471
chr2.2 exon 5070973 5071283 5070973 ID=MS.gene78471.t1.exon1;Parent=MS.gene78471.t1
chr2.2 CDS 5070973 5071283 5070973 ID=cds.MS.gene78471.t1;Parent=MS.gene78471.t1
chr2.2 exon 5072154 5072317 5072154 ID=MS.gene78471.t1.exon2;Parent=MS.gene78471.t1
chr2.2 CDS 5072154 5072317 5072154 ID=cds.MS.gene78471.t1;Parent=MS.gene78471.t1
chr2.2 exon 5072524 5072687 5072524 ID=MS.gene78471.t1.exon3;Parent=MS.gene78471.t1
chr2.2 CDS 5072524 5072687 5072524 ID=cds.MS.gene78471.t1;Parent=MS.gene78471.t1
chr2.2 exon 5072808 5073070 5072808 ID=MS.gene78471.t1.exon4;Parent=MS.gene78471.t1
chr2.2 CDS 5072808 5073070 5072808 ID=cds.MS.gene78471.t1;Parent=MS.gene78471.t1
chr2.2 exon 5073226 5073352 5073226 ID=MS.gene78471.t1.exon5;Parent=MS.gene78471.t1
chr2.2 CDS 5073226 5073352 5073226 ID=cds.MS.gene78471.t1;Parent=MS.gene78471.t1
Gene Sequence

>MS.gene78471

ATGAGTGGAGGGAGGAACCCACATGGTGGCACCACCGTTGAGGTTAAGGTTCATCCTCGCCGTGGGTCTCCGTCTTCGTCGTCGTCGAATCCTCCCGTCACTCGTGATCGTGATTTCAGGCTCTTTAAGCAATGGGTTCCATGGCTTGTTCCAACTTTTGTGTTTGCCAACGTTATTATTTTCATTGTTACAATGTATGTTAATGATTGTCCTGAGAATGCATTTCATGGTACTTGTGTTGCTCCTTTTCTTGGAAGATTCTCCTTTCAACCACTTAAAGAAAACCCTCTCTTTGGTCCTTCTTCCTTTACGTGAGTTTTCTTTTTCTCTTTATCTTTTCATCTTATCATACAATACAATTACAATGTTTATTTTTTTTTTATTTTATTTTAATTACAATTTTACATTAATCAAGTTATGATTGGTTGGTTCTTATGATGCATGTAGTTACATTCAATTATTCTATTTTCTCAAATTATTACATGTGTTGATGTTGTTAAGAGTCTTGCATTAGATGAGATGTAGCTTGAGAATGAGTTTATTAGTGTGGATAATCGTCATTTTGCAAGCCAGTTTTGTGAATGAGTTAGGCATAATTGAAATTTTAAGATGTATCAGAGTTTTTCCAAGATCCATTGAACCGCCTGCTATCTGGCCACCATTATCAGGCCACACGAGTCACACTTTAGATGTCTAAGATTGGGGGTAAGGGTGTGTTAAGAGTCTCACATTGAATGAGATAGACATGAACATGTGTTTATAAATGGGGGGTTGGGTAGTTAAATTGATTCGAGCTAAGGGATAAGAAGTTGGAGGTTCTAAGTTCAAACCCGGACGAAGGAGTAAATATTAATATAACAACTAATTGCTAACACTTGTCATTCAGAAACAAAAACGTGTGTTTGTAAGGATGCATAGGTTCAATATAAAAAACCTAATAGATGTGTGAGGGTTGTGTTTATCTTGTGTCTGTGTCTCTACCGGTGATTCATAGATTACATTCTCTATGGCTATGAAATTTCAGAAATGACTAAAATTTTGAGAGTTTTCAACTCAAATTCAGTCCAAATTATGTATTTGCAATAGCTAATGGACAGTACCAATCTTAATTTTAGGCAGATATATCTCTTGGAGATGTTTTTTATGTGCTGACGAAAACTATTTATTACTCGCATTTTCAGATTACAGGAGATGGGTGCTCTAGAAGTAGATAAAGTGGTTCATGAACACGAAGGTTGGCGCCTTGTCTCTTGTATATGGTTGCATGGTGGGGCTATCCATGTACTTGCCAACATGTTGAGTCTTCTTTTTATTGGAATTCGGCTTGAACAAGAATTTGGATTTGGTATGGTTCTGGTAATTATCCTTGCATTGTTCAAGTATATCGTTATATTTCTTCCGACCAACAGTATTCCATTATCTGTATTTATATCATTGTTTTTACATAAATGTTCTGGAAGAAAAGAAACTCAGCTGAGTTTATTTAGCAATGAAATCAATAAAAAAGCTTTGTTGTCGATTGTAATTTCTACCTTTCTTGTCCTTTCGCAGTTAGAATTGGATTTCTCTATGTGATATCTGGTTTTGGAGGGAGTTTGCTGTCTGCGTTATTCATACAAACGGGCATCTCAGTTGGTGCATCTGGTGCATTGTTTGGCTTACTCGGAGGGATGCTCTCAGAGCTCTTCATTAATTGGACGATATATGCCAATAAGGTGAATGAACTAAAAAAATTGTTCAATTCAACAGTGCTTCCTGATAAATAATTTATTGTCTTTGCGGTCTGCGCCTAAACCATTCTATTTCTTTCCATTTTGTCTCATGATGTTTTGCAGATGGCTGCATTGTTGACTCTTATAGTCATTGTTGTAATAAATTTAGCTGTTGGAGTCCTTCCACATGTTGACAATTTCGCTCATCTAGGAGGATTTGGTTCTGGCTTTCTGCTTGGATTTGTCCTTTTGATTCGTCCTCAGTTTAAATGGATAAGCCAACAACAGTCTAAATCTGGATCTCATGCATTTTCTGTTAAACACAATAAGTACTTACCTTATCAATATGTGTTGTGGGTTTTGTCTTTCCTATTACTCATTGCAGGGTAAGTTTGTTTTAGTTTTCTTTTCTCTTTTGTATATTACCTTTAATATTCTTAGGACTTGGATACTCTTTTCACAAATATTTTGTTTGTAGAGATTGAATTTATGTTATTGCTTGTAAAGTAGTATTTAATCTAAAGTTGGATAATTTTGACAGATTCATTACTGGGATGGTTCTACTCTTTCGAGGTGTTAACTTGAATGAACGTTGCTCGTGGTGTCATTATTTAAGTTGTGTTCCTACCTCAAAATGGAGTTGTCAGGAACAAAGCATTTATTGTGAG
Protein sequence

>MS.gene78471.t1

MSGGRNPHGGTTVEVKVHPRRGSPSSSSSNPPVTRDRDFRLFKQWVPWLVPTFVFANVIIFIVTMYVNDCPENAFHGTCVAPFLGRFSFQPLKENPLFGPSSFTLQEMGALEVDKVVHEHEGWRLVSCIWLHGGAIHVLANMLSLLFIGIRLEQEFGFVRIGFLYVISGFGGSLLSALFIQTGISVGASGALFGLLGGMLSELFINWTIYANKMAALLTLIVIVVINLAVGVLPHVDNFAHLGGFGSGFLLGFVLLIRPQFKWISQQQSKSGSHAFSVKHNKYLPYQYVLWVLSFLLLIAGFITGMVLLFRGVNLNERCSWCHYLSCVPTSKWSCQEQSIYCE