AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene79380


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene79380.t1 MTR_3g026230 77.593 241 15 6 1 204 14 252 1.04e-88 261
MS.gene79380.t1 MTR_3g026160 90.244 164 14 1 1 162 14 177 1.53e-78 233
MS.gene79380.t1 MTR_3g026160 61.364 88 20 2 110 197 35 108 3.82e-17 75.9
MS.gene79380.t1 MTR_3g026330 65.888 214 47 3 1 198 14 217 6.80e-67 209
MS.gene79380.t1 MTR_3g026330 65.829 199 45 2 27 204 148 344 4.35e-55 178
MS.gene79380.t1 MTR_3g026330 66.667 195 40 3 26 197 101 293 7.86e-53 172
MS.gene79380.t1 MTR_3g026330 65.289 121 34 3 20 140 225 337 2.49e-26 103
MS.gene79380.t1 MTR_3g026290 64.840 219 46 3 1 198 14 222 2.83e-65 205
MS.gene79380.t1 MTR_3g026290 62.981 208 47 2 27 204 153 360 6.54e-53 173
MS.gene79380.t1 MTR_3g026290 65.517 145 41 3 25 163 213 354 9.09e-32 118
MS.gene79380.t1 MTR_3g026290 65.455 110 32 3 23 129 247 353 6.33e-21 89.4
MS.gene79380.t1 MTR_3g026290 60.169 118 25 3 23 140 258 353 1.70e-20 88.2
MS.gene79380.t1 MTR_3g026240 53.191 94 39 2 1 94 14 102 2.26e-20 82.8
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene79380.t1 AT5G09530 59.545 220 51 6 13 197 30 246 1.34e-49 165
MS.gene79380.t1 AT5G09530 64.021 189 52 5 22 197 100 285 1.05e-45 155
MS.gene79380.t1 AT5G09530 62.827 191 55 5 19 197 179 365 1.06e-42 147
MS.gene79380.t1 AT5G09530 56.886 167 44 5 24 163 201 366 1.85e-29 112
MS.gene79380.t1 AT5G09530 60.440 91 28 2 22 106 282 370 1.29e-15 74.7
MS.gene79380.t1 AT5G09530 66.176 68 21 1 137 204 39 104 9.05e-11 60.5
MS.gene79380.t1 AT5G09520 45.517 145 47 3 1 145 16 128 1.45e-22 89.0
MS.gene79380.t1 AT5G09520 58.000 100 32 2 97 196 41 130 1.38e-20 83.6
MS.gene79380.t1 AT5G09520 54.651 86 35 1 123 204 34 119 1.75e-14 67.8

Find 70 sgRNAs with CRISPR-Local

Find 79 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TCAGGTACCTTTGGTATTTC+TGG 0.077856 3.1:-17092613 None:intergenic
GGAACATCTTTAGGAGTTTC+AGG 0.202854 3.1:-17092736 None:intergenic
TCAGGTACCTTAGGCAATTC+TGG 0.231204 3.1:-17092307 None:intergenic
TCAGGTACCTTAGGTAATTC+TGG 0.242157 3.1:-17092340 None:intergenic
TTAGGAGTTTCAGGGAATTT+AGG 0.242346 3.1:-17092727 None:intergenic
AACTCTAGCTTTGGTAATTC+AGG 0.247874 3.1:-17092631 None:intergenic
AATTCTGGCTTTGGCAATTC+AGG 0.265103 3.1:-17092325 None:intergenic
ACTTTAGGTTGCTCGGGTTT+TGG 0.275487 3.1:-17092475 None:intergenic
AGTTCAGGCTTTGTCAATTC+AGG 0.281138 3.1:-17092664 None:intergenic
TTTGGCAATTCAGGGACTTT+AGG 0.288403 3.1:-17092490 None:intergenic
TCAGGTACCTTAGGGATATC+TGG 0.309626 3.1:-17092547 None:intergenic
TTTGTCAATTCAGGTATCTT+AGG 0.317967 3.1:-17092655 None:intergenic
TTAGGCAATTCAGGAATCTT+AGG 0.318033 3.1:-17092283 None:intergenic
TACCTTTGGTATTTCTGGCT+TGG 0.319635 3.1:-17092608 None:intergenic
AGCTCAGGCTTTGGAAGTTC+AGG 0.319717 3.1:-17092358 None:intergenic
AATTTAGGTAACTCGGGTTT+TGG 0.322053 3.1:-17092712 None:intergenic
ACCTTTGGTATTTCTGGCTT+GGG 0.325986 3.1:-17092607 None:intergenic
GTATTGGTTGGAACATCTTT+AGG 0.328990 3.1:-17092745 None:intergenic
TTAGGCAACTCGGGAACTTT+AGG 0.329308 3.1:-17092439 None:intergenic
TACCTTAGGGATATCTGGTT+TGG 0.332667 3.1:-17092542 None:intergenic
ATAGGTGGAAAATCAAGTTT+TGG 0.353325 3.1:-17092244 None:intergenic
ACTTTAGGTAGTTCATGTTT+TGG 0.353892 3.1:-17092508 None:intergenic
ACTTTGGGAAACTCGGGTTT+TGG 0.357337 3.1:-17092406 None:intergenic
TTAGGCAACTCAGGAACTTT+GGG 0.358876 3.1:-17092421 None:intergenic
TTGGGCAACTCAGAGACTTT+AGG 0.363342 3.1:-17092523 None:intergenic
ACCTTAGGTAATTCTGGCTT+TGG 0.366886 3.1:-17092334 None:intergenic
TTGGGCAACTCAGAGACTTT+TGG 0.369313 3.1:-17092589 None:intergenic
ACATTAACTTTAGGTAGTTC+AGG 0.370017 3.1:-17092679 None:intergenic
GGAAGCTCAGGCTTTGGTAT+AGG 0.376080 3.1:-17092262 None:intergenic
TTCAGGGACTTTAGGTTGCT+CGG 0.381939 3.1:-17092482 None:intergenic
AATTCTGGCTTAGGCAATTC+AGG 0.383466 3.1:-17092292 None:intergenic
AGTTCAGGGACATTAACTTT+AGG 0.387475 3.1:-17092688 None:intergenic
CTCAGGAACTTTGGGAAACT+CGG 0.389158 3.1:-17092413 None:intergenic
TTTAGGCAACTCAGGAACTT+TGG 0.393082 3.1:-17092422 None:intergenic
ATCTTAGGAAGCTCAGGCTT+TGG 0.394512 3.1:-17092268 None:intergenic
TTTGGTAATTCAGGTACCTT+TGG 0.399783 3.1:-17092622 None:intergenic
AACTTAGGTAGCTCAGGCTT+TGG 0.406351 3.1:-17092367 None:intergenic
TTCGGTAACTCAGGTACCTT+AGG 0.407298 3.1:-17092556 None:intergenic
TTTGGAAGTTCAGGTACCTT+AGG 0.422522 3.1:-17092349 None:intergenic
ACCTTAGGGATATCTGGTTT+GGG 0.423518 3.1:-17092541 None:intergenic
ATCTTAGGTAACTCTAGCTT+TGG 0.432161 3.1:-17092640 None:intergenic
AGTTCCAACTTAAGGAGTAT+TGG 0.438613 3.1:-17092761 None:intergenic
AGAGTATTACAATGGTTGTC+GGG 0.440611 3.1:+17092185 MS.gene79380:CDS
TTTGGCAATTCAGGTACCTT+AGG 0.442441 3.1:-17092316 None:intergenic
TCAGGAATCTTAGGAAGCTC+AGG 0.462216 3.1:-17092274 None:intergenic
ACATTAAACTTAGGTAGCTC+AGG 0.466667 3.1:-17092373 None:intergenic
TTTGGCAATTCAGGGAACTT+AGG 0.478230 3.1:-17092457 None:intergenic
TTCAGGGAATTTAGGTAACT+CGG 0.478958 3.1:-17092719 None:intergenic
GACAACCATTGTAATACTCT+TGG 0.484545 3.1:-17092182 None:intergenic
ACCTTAGGCAATTCTGGCTT+AGG 0.484577 3.1:-17092301 None:intergenic
AATTCAGGAACATTAAACTT+AGG 0.490699 3.1:-17092382 None:intergenic
GAACATCTTTAGGAGTTTCA+GGG 0.495266 3.1:-17092735 None:intergenic
TTCAGGGAACTTAGGCAACT+CGG 0.499436 3.1:-17092449 None:intergenic
TCAGGGACTTTAGGTTGCTC+GGG 0.512283 3.1:-17092481 None:intergenic
CCAACTTAAGGAGTATTGGT+TGG 0.525013 3.1:-17092757 None:intergenic
AAGAGTATTACAATGGTTGT+CGG 0.526376 3.1:+17092184 MS.gene79380:CDS
TCAGGAACTTTGGGAAACTC+GGG 0.528389 3.1:-17092412 None:intergenic
AGCTCAGGCTTTGGTATAGG+TGG 0.530445 3.1:-17092259 None:intergenic
TCGGTAACTCAGGTACCTTA+GGG 0.543706 3.1:-17092555 None:intergenic
TCAGGGAATTTAGGTAACTC+GGG 0.549724 3.1:-17092718 None:intergenic
TCGGGAACTTTAGGCAACTC+AGG 0.569614 3.1:-17092430 None:intergenic
AGCTCGGGTTTCGGTAACTC+AGG 0.578248 3.1:-17092565 None:intergenic
TCAGGGAACTTAGGCAACTC+GGG 0.582113 3.1:-17092448 None:intergenic
GCCAAAGCCAGAATTACCTA+AGG 0.599842 3.1:+17092333 MS.gene79380:CDS
GCCTAAGCCAGAATTGCCTA+AGG 0.613699 3.1:+17092300 MS.gene79380:CDS
GAGTATTACAATGGTTGTCG+GGG 0.633099 3.1:+17092186 MS.gene79380:CDS
GCCCAAACCAGATATCCCTA+AGG 0.649847 3.1:+17092540 MS.gene79380:CDS
GTCAGCCAAGAGTATTACAA+TGG 0.663719 3.1:+17092177 MS.gene79380:CDS
GCCCAAGCCAGAAATACCAA+AGG 0.675655 3.1:+17092606 MS.gene79380:CDS
TCTAGAGTCGAACATACCTG+AGG 0.701502 3.1:+17092219 MS.gene79380:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
! AATTCAGGAACATTAAACTT+AGG - chr3.1:17092385-17092404 None:intergenic 25.0%
ACATTAACTTTAGGTAGTTC+AGG - chr3.1:17092682-17092701 None:intergenic 30.0%
TTTGTCAATTCAGGTATCTT+AGG - chr3.1:17092658-17092677 None:intergenic 30.0%
! ACTTTAGGTAGTTCATGTTT+TGG - chr3.1:17092511-17092530 None:intergenic 30.0%
! ATAGGTGGAAAATCAAGTTT+TGG - chr3.1:17092247-17092266 None:intergenic 30.0%
!! AAGAGTATTACAATGGTTGT+CGG + chr3.1:17092184-17092203 MS.gene79380:CDS 30.0%
ACATTAAACTTAGGTAGCTC+AGG - chr3.1:17092376-17092395 None:intergenic 35.0%
AGTTCAGGGACATTAACTTT+AGG - chr3.1:17092691-17092710 None:intergenic 35.0%
GACAACCATTGTAATACTCT+TGG - chr3.1:17092185-17092204 None:intergenic 35.0%
TTAGGAGTTTCAGGGAATTT+AGG - chr3.1:17092730-17092749 None:intergenic 35.0%
TTAGGCAATTCAGGAATCTT+AGG - chr3.1:17092286-17092305 None:intergenic 35.0%
TTCAGGGAATTTAGGTAACT+CGG - chr3.1:17092722-17092741 None:intergenic 35.0%
! AATTTAGGTAACTCGGGTTT+TGG - chr3.1:17092715-17092734 None:intergenic 35.0%
! AGAGTATTACAATGGTTGTC+GGG + chr3.1:17092185-17092204 MS.gene79380:CDS 35.0%
! ATCTTAGGTAACTCTAGCTT+TGG - chr3.1:17092643-17092662 None:intergenic 35.0%
! GAACATCTTTAGGAGTTTCA+GGG - chr3.1:17092738-17092757 None:intergenic 35.0%
! TTTGGTAATTCAGGTACCTT+TGG - chr3.1:17092625-17092644 None:intergenic 35.0%
!! AACTCTAGCTTTGGTAATTC+AGG - chr3.1:17092634-17092653 None:intergenic 35.0%
!! GTATTGGTTGGAACATCTTT+AGG - chr3.1:17092748-17092767 None:intergenic 35.0%
!!! AAATCAAGTTTTGGAACCTC+AGG - chr3.1:17092238-17092257 None:intergenic 35.0%
!!! AGTTCATGTTTTGGCAATTC+AGG - chr3.1:17092502-17092521 None:intergenic 35.0%
!!! GTTCATGTTTTGGCAATTCA+GGG - chr3.1:17092501-17092520 None:intergenic 35.0%
GAGTATTACAATGGTTGTCG+GGG + chr3.1:17092186-17092205 MS.gene79380:CDS 40.0%
TACCTTAGGGATATCTGGTT+TGG - chr3.1:17092545-17092564 None:intergenic 40.0%
TCAGGGAATTTAGGTAACTC+GGG - chr3.1:17092721-17092740 None:intergenic 40.0%
TCAGGTACCTTAGGTAATTC+TGG - chr3.1:17092343-17092362 None:intergenic 40.0%
TCAGGTACCTTTGGTATTTC+TGG - chr3.1:17092616-17092635 None:intergenic 40.0%
TTAGGCAACTCAGGAACTTT+GGG - chr3.1:17092424-17092443 None:intergenic 40.0%
TTTAGGCAACTCAGGAACTT+TGG - chr3.1:17092425-17092444 None:intergenic 40.0%
TTTGGAAGTTCAGGTACCTT+AGG - chr3.1:17092352-17092371 None:intergenic 40.0%
TTTGGCAATTCAGGGAACTT+AGG - chr3.1:17092460-17092479 None:intergenic 40.0%
TTTGGCAATTCAGGGACTTT+AGG - chr3.1:17092493-17092512 None:intergenic 40.0%
TTTGGCAATTCAGGTACCTT+AGG - chr3.1:17092319-17092338 None:intergenic 40.0%
! ACCTTAGGGATATCTGGTTT+GGG - chr3.1:17092544-17092563 None:intergenic 40.0%
! AGTTCAGGCTTTGTCAATTC+AGG - chr3.1:17092667-17092686 None:intergenic 40.0%
! GGAACATCTTTAGGAGTTTC+AGG - chr3.1:17092739-17092758 None:intergenic 40.0%
!! AATTCTGGCTTAGGCAATTC+AGG - chr3.1:17092295-17092314 None:intergenic 40.0%
!! AATTCTGGCTTTGGCAATTC+AGG - chr3.1:17092328-17092347 None:intergenic 40.0%
!! ACCTTAGGTAATTCTGGCTT+TGG - chr3.1:17092337-17092356 None:intergenic 40.0%
!! ACCTTTGGTATTTCTGGCTT+GGG - chr3.1:17092610-17092629 None:intergenic 40.0%
!! GTCAGCCAAGAGTATTACAA+TGG + chr3.1:17092177-17092196 MS.gene79380:CDS 40.0%
!! TACCTTTGGTATTTCTGGCT+TGG - chr3.1:17092611-17092630 None:intergenic 40.0%
AACTTAGGTAGCTCAGGCTT+TGG - chr3.1:17092370-17092389 None:intergenic 45.0%
ATCTTAGGAAGCTCAGGCTT+TGG - chr3.1:17092271-17092290 None:intergenic 45.0%
GCCAAAGCCAGAATTACCTA+AGG + chr3.1:17092333-17092352 MS.gene79380:CDS 45.0%
TCAGGAATCTTAGGAAGCTC+AGG - chr3.1:17092277-17092296 None:intergenic 45.0%
TCAGGTACCTTAGGCAATTC+TGG - chr3.1:17092310-17092329 None:intergenic 45.0%
TCAGGTACCTTAGGGATATC+TGG - chr3.1:17092550-17092569 None:intergenic 45.0%
TCGGTAACTCAGGTACCTTA+GGG - chr3.1:17092558-17092577 None:intergenic 45.0%
TTAGGCAACTCGGGAACTTT+AGG - chr3.1:17092442-17092461 None:intergenic 45.0%
TTCAGGGAACTTAGGCAACT+CGG - chr3.1:17092452-17092471 None:intergenic 45.0%
TTCGGTAACTCAGGTACCTT+AGG - chr3.1:17092559-17092578 None:intergenic 45.0%
TTGGGCAACTCAGAGACTTT+AGG - chr3.1:17092526-17092545 None:intergenic 45.0%
! CTCAGGAACTTTGGGAAACT+CGG - chr3.1:17092416-17092435 None:intergenic 45.0%
! TCAGGAACTTTGGGAAACTC+GGG - chr3.1:17092415-17092434 None:intergenic 45.0%
! TCTAGAGTCGAACATACCTG+AGG + chr3.1:17092219-17092238 MS.gene79380:CDS 45.0%
! TTGGGCAACTCAGAGACTTT+TGG - chr3.1:17092526-17092545 None:intergenic 45.0%
!! AACTCGGGTTTTGGCAATTC+AGG - chr3.1:17092400-17092419 None:intergenic 45.0%
!! ACCTTAGGCAATTCTGGCTT+AGG - chr3.1:17092304-17092323 None:intergenic 45.0%
!! ACTTTGGGAAACTCGGGTTT+TGG - chr3.1:17092409-17092428 None:intergenic 45.0%
!! ACTTTTGGTAGCTCGGGTTT+CGG - chr3.1:17092577-17092596 None:intergenic 45.0%
!! CTCAGAGACTTTTGGTAGCT+CGG - chr3.1:17092584-17092603 None:intergenic 45.0%
!! TCAGAGACTTTTGGTAGCTC+GGG - chr3.1:17092583-17092602 None:intergenic 45.0%
!! TTCAGGGACTTTAGGTTGCT+CGG - chr3.1:17092485-17092504 None:intergenic 45.0%
!!! ACTTTAGGTTGCTCGGGTTT+TGG - chr3.1:17092478-17092497 None:intergenic 45.0%
GCCCAAACCAGATATCCCTA+AGG + chr3.1:17092540-17092559 MS.gene79380:CDS 50.0%
GCCCAAGCCAGAAATACCAA+AGG + chr3.1:17092606-17092625 MS.gene79380:CDS 50.0%
GCCTAAGCCAGAATTGCCTA+AGG + chr3.1:17092300-17092319 MS.gene79380:CDS 50.0%
TCAGGGAACTTAGGCAACTC+GGG - chr3.1:17092451-17092470 None:intergenic 50.0%
TCGGGAACTTTAGGCAACTC+AGG - chr3.1:17092433-17092452 None:intergenic 50.0%
! AGCTCAGGCTTTGGTATAGG+TGG - chr3.1:17092262-17092281 None:intergenic 50.0%
! GGAAGCTCAGGCTTTGGTAT+AGG - chr3.1:17092265-17092284 None:intergenic 50.0%
!! AACTCGGGTTTTGGCAGTTC+AGG - chr3.1:17092706-17092725 None:intergenic 50.0%
!! ACTCGGGTTTTGGCAGTTCA+GGG - chr3.1:17092705-17092724 None:intergenic 50.0%
!! AGCTCAGGCTTTGGAAGTTC+AGG - chr3.1:17092361-17092380 None:intergenic 50.0%
!! GCTCGGGTTTTGGCAATTCA+GGG - chr3.1:17092468-17092487 None:intergenic 50.0%
!! TCAGGGACTTTAGGTTGCTC+GGG - chr3.1:17092484-17092503 None:intergenic 50.0%
!! TGCTCGGGTTTTGGCAATTC+AGG - chr3.1:17092469-17092488 None:intergenic 50.0%
AGCTCGGGTTTCGGTAACTC+AGG - chr3.1:17092568-17092587 None:intergenic 55.0%


Chromosome Type Strat End Strand Name
chr3.1 gene 17092160 17092774 17092160 ID=MS.gene79380
chr3.1 mRNA 17092160 17092774 17092160 ID=MS.gene79380.t1;Parent=MS.gene79380
chr3.1 exon 17092160 17092774 17092160 ID=MS.gene79380.t1.exon1;Parent=MS.gene79380.t1
chr3.1 CDS 17092160 17092774 17092160 ID=cds.MS.gene79380.t1;Parent=MS.gene79380.t1
Gene Sequence

>MS.gene79380

ATGATTCTAGCTATACTGTCAGCCAAGAGTATTACAATGGTTGTCGGGGCACGCAATCTTCTAGAGTCGAACATACCTGAGGTTCCAAAACTTGATTTTCCACCTATACCAAAGCCTGAGCTTCCTAAGATTCCTGAATTGCCTAAGCCAGAATTGCCTAAGGTACCTGAATTGCCAAAGCCAGAATTACCTAAGGTACCTGAACTTCCAAAGCCTGAGCTACCTAAGTTTAATGTTCCTGAATTGCCAAAACCCGAGTTTCCCAAAGTTCCTGAGTTGCCTAAAGTTCCCGAGTTGCCTAAGTTCCCTGAATTGCCAAAACCCGAGCAACCTAAAGTCCCTGAATTGCCAAAACATGAACTACCTAAAGTCTCTGAGTTGCCCAAACCAGATATCCCTAAGGTACCTGAGTTACCGAAACCCGAGCTACCAAAAGTCTCTGAGTTGCCCAAGCCAGAAATACCAAAGGTACCTGAATTACCAAAGCTAGAGTTACCTAAGATACCTGAATTGACAAAGCCTGAACTACCTAAAGTTAATGTCCCTGAACTGCCAAAACCCGAGTTACCTAAATTCCCTGAAACTCCTAAAGATGTTCCAACCAATACTCCTTAA

Protein sequence

>MS.gene79380.t1

MILAILSAKSITMVVGARNLLESNIPEVPKLDFPPIPKPELPKIPELPKPELPKVPELPKPELPKVPELPKPELPKFNVPELPKPEFPKVPELPKVPELPKFPELPKPEQPKVPELPKHELPKVSELPKPDIPKVPELPKPELPKVSELPKPEIPKVPELPKLELPKIPELTKPELPKVNVPELPKPELPKFPETPKDVPTNTP