Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene053568.t1 | XP_003608424.1 | 97.1 | 35 | 1 | 0 | 1 | 35 | 1 | 35 | 3.00E-06 | 61.6 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene053568.t1 | G7JV73 | 97.1 | 35 | 1 | 0 | 1 | 35 | 1 | 35 | 2.2e-06 | 61.6 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene050082 | MS.gene053568 | 0.882755 | 7.98E-71 | -1.69E-46 |
| MS.gene053568 | MS.gene053569 | 0.936446 | 1.71E-97 | -1.69E-46 |
| MS.gene053568 | MS.gene057717 | 0.810586 | 1.07E-50 | -1.69E-46 |
| MS.gene053568 | MS.gene058068 | 0.834481 | 2.92E-56 | -1.69E-46 |
| MS.gene053568 | MS.gene061044 | 0.81161 | 6.42E-51 | -1.69E-46 |
| MS.gene053568 | MS.gene061777 | 0.824465 | 7.95E-54 | -1.69E-46 |
| MS.gene053568 | MS.gene068034 | 0.935147 | 1.34E-96 | -1.69E-46 |
| MS.gene053568 | MS.gene068035 | 0.98617 | 6.67E-166 | -1.69E-46 |
| MS.gene053568 | MS.gene068037 | 0.860523 | 1.95E-63 | -1.69E-46 |
| MS.gene053568 | MS.gene09819 | 0.835812 | 1.35E-56 | -1.69E-46 |
| MS.gene053568 | MS.gene27942 | 0.800871 | 1.18E-48 | -1.69E-46 |
| MS.gene053568 | MS.gene27964 | 0.800201 | 1.61E-48 | -1.69E-46 |
| MS.gene053568 | MS.gene28242 | 0.82277 | 1.98E-53 | -1.69E-46 |
| MS.gene053568 | MS.gene28244 | 0.836401 | 9.56E-57 | -1.69E-46 |
| MS.gene053568 | MS.gene28537 | 0.811566 | 6.57E-51 | -1.69E-46 |
| MS.gene053568 | MS.gene29505 | 0.800472 | 1.42E-48 | -1.69E-46 |
| MS.gene053568 | MS.gene32534 | 0.802732 | 4.88E-49 | -1.69E-46 |
| MS.gene053568 | MS.gene39959 | 0.832172 | 1.10E-55 | -1.69E-46 |
| MS.gene053568 | MS.gene44966 | 0.84478 | 6.11E-59 | -1.69E-46 |
| MS.gene053568 | MS.gene50534 | 0.83206 | 1.17E-55 | -1.69E-46 |
| MS.gene053568 | MS.gene53494 | 0.802327 | 5.91E-49 | -1.69E-46 |
| MS.gene053568 | MS.gene57291 | 0.825601 | 4.29E-54 | -1.69E-46 |
| MS.gene053568 | MS.gene57869 | 0.80051 | 1.39E-48 | -1.69E-46 |
| MS.gene053568 | MS.gene58425 | 0.803165 | 3.97E-49 | -1.69E-46 |
| MS.gene053568 | MS.gene64069 | 0.883838 | 3.20E-71 | -1.69E-46 |
| MS.gene053568 | MS.gene67222 | 0.800516 | 1.39E-48 | -1.69E-46 |
| MS.gene053568 | MS.gene75261 | 0.816115 | 6.53E-52 | -1.69E-46 |
| MS.gene053568 | MS.gene76883 | 0.803055 | 4.18E-49 | -1.69E-46 |
| MS.gene053568 | MS.gene78768 | 0.822208 | 2.68E-53 | -1.69E-46 |
| MS.gene053568 | MS.gene79368 | 0.820577 | 6.37E-53 | -1.69E-46 |
| MS.gene053568 | MS.gene79380 | 0.845141 | 4.89E-59 | -1.69E-46 |
| MS.gene053568 | MS.gene79392 | 0.801996 | 6.92E-49 | -1.69E-46 |
| MS.gene053568 | MS.gene88084 | 0.802609 | 5.17E-49 | -1.69E-46 |
| MS.gene053568 | MS.gene90207 | 0.815632 | 8.37E-52 | -1.69E-46 |
| MS.gene053568 | MS.gene90209 | 0.989021 | 2.29E-176 | -1.69E-46 |
| MS.gene053568 | MS.gene90210 | 0.944796 | 1.01E-103 | -1.69E-46 |
| MS.gene053568 | MS.gene90969 | 0.844674 | 6.53E-59 | -1.69E-46 |
| MS.gene053568 | MS.gene91317 | 0.836145 | 1.11E-56 | -1.69E-46 |
| MS.gene053568 | MS.gene93357 | 0.816652 | 4.95E-52 | -1.69E-46 |
| MS.gene053568 | MS.gene93545 | 0.807389 | 5.18E-50 | -1.69E-46 |
| MS.gene053568 | MS.gene93574 | 0.816608 | 5.07E-52 | -1.69E-46 |
| MS.gene053568 | MS.gene93589 | 0.828638 | 8.04E-55 | -1.69E-46 |
| MS.gene053568 | MS.gene96701 | 0.836848 | 7.36E-57 | -1.69E-46 |
| MS.gene053568 | MS.gene98021 | 0.826426 | 2.73E-54 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene053568.t1 | MTR_4g093940 | 93.750 | 128 | 3 | 1 | 1 | 128 | 1 | 123 | 1.89e-67 | 199 |
| MS.gene053568.t1 | MTR_4g093950 | 93.750 | 112 | 7 | 0 | 1 | 112 | 1 | 112 | 1.12e-49 | 155 |
| MS.gene053568.t1 | MTR_4g093910 | 92.857 | 112 | 8 | 0 | 1 | 112 | 1 | 112 | 1.10e-48 | 152 |
| MS.gene053568.t1 | MTR_4g093920 | 91.743 | 109 | 8 | 1 | 1 | 109 | 1 | 108 | 2.19e-47 | 149 |
| MS.gene053568.t1 | MTR_5g064530 | 65.132 | 152 | 27 | 4 | 1 | 128 | 1 | 150 | 8.34e-42 | 135 |
| MS.gene053568.t1 | MTR_5g064510 | 66.667 | 123 | 21 | 4 | 1 | 105 | 1 | 121 | 4.06e-36 | 121 |
| MS.gene053568.t1 | MTR_4g094678 | 69.620 | 79 | 6 | 3 | 17 | 92 | 1 | 64 | 1.75e-22 | 84.3 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 44 sgRNAs with CRISPR-Local
Find 52 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| AGATTTCCTTGAGGCATATT+TGG | 0.193132 | 4.4:+21276888 | None:intergenic |
| GTGGGAATGGTAGGTAAATT+TGG | 0.271860 | 4.4:+21276951 | None:intergenic |
| GCAATAGATGGCGAGCTTCT+AGG | 0.294792 | 4.4:+21276988 | None:intergenic |
| GGTGGTAATGTAGTTGGTTT+TGG | 0.298989 | 4.4:+21276924 | None:intergenic |
| TAAAGCTGCAATGAAGGATT+TGG | 0.355352 | 4.4:+21277040 | None:intergenic |
| AGATGGAATTGTTGGAATAT+TGG | 0.374933 | 4.4:+21276800 | None:intergenic |
| GTGTTGTTGTTTGCAATAGA+TGG | 0.376065 | 4.4:+21276976 | None:intergenic |
| GGGAAAGTGGTTGGGATTGA+AGG | 0.382492 | 4.4:+21276732 | None:intergenic |
| GGCTAGAAGTTGAAGGTGGT+GGG | 0.405223 | 4.4:+21276691 | None:intergenic |
| TGAGGCATATTTGGAATTGA+AGG | 0.405314 | 4.4:+21276897 | None:intergenic |
| ATAGTGAGTTTAGGCATAGA+AGG | 0.436994 | 4.4:+21276846 | None:intergenic |
| GGGAAGGAGGGGAAAGTGGT+TGG | 0.439412 | 4.4:+21276723 | None:intergenic |
| GGAAGGAGGGGAAAGTGGTT+GGG | 0.444157 | 4.4:+21276724 | None:intergenic |
| GGCAAAGGTGGTAATGTAGT+TGG | 0.444412 | 4.4:+21276918 | None:intergenic |
| GGTTGGGATTGAAGGAAGGT+TGG | 0.454159 | 4.4:+21276740 | None:intergenic |
| AGTGGTGGCATAGTGAGTTT+AGG | 0.457061 | 4.4:+21276837 | None:intergenic |
| AAATGGGAAGGAGGGGAAAG+TGG | 0.467197 | 4.4:+21276719 | None:intergenic |
| GTTGGGATTGAAGGAAGGTT+GGG | 0.469467 | 4.4:+21276741 | None:intergenic |
| TTAGGCATAGAAGGGATAGT+AGG | 0.471087 | 4.4:+21276855 | None:intergenic |
| GGGCTAGAAGTTGAAGGTGG+TGG | 0.472490 | 4.4:+21276690 | None:intergenic |
| AAGAAGTAAAGCTGCAATGA+AGG | 0.485067 | 4.4:+21277034 | None:intergenic |
| GTTTAAGGGCTAGAAGTTGA+AGG | 0.490262 | 4.4:+21276684 | None:intergenic |
| GGAATATTGGTAGGAAGACT+TGG | 0.503031 | 4.4:+21276813 | None:intergenic |
| AAGTGGTTGGGATTGAAGGA+AGG | 0.508086 | 4.4:+21276736 | None:intergenic |
| TGCAATGAAGGATTTGGTTG+AGG | 0.510590 | 4.4:+21277046 | None:intergenic |
| TTTGGTAAAGTGGGAATGGT+AGG | 0.519398 | 4.4:+21276942 | None:intergenic |
| AGTGAAGTGGCTGCTGGCAA+TGG | 0.520284 | 4.4:+21276765 | None:intergenic |
| GGAATTGTTGGAATATTGGT+AGG | 0.523994 | 4.4:+21276804 | None:intergenic |
| ATGTTGAGAGATGGAATTGT+TGG | 0.529497 | 4.4:+21276792 | None:intergenic |
| TTGGTAGGAAGACTTGGAAG+TGG | 0.530626 | 4.4:+21276819 | None:intergenic |
| GAAGGGATAGTAGGCAATGG+AGG | 0.560608 | 4.4:+21276864 | None:intergenic |
| GAAGTGGCTGCTGGCAATGG+TGG | 0.580562 | 4.4:+21276768 | None:intergenic |
| TTTGGAATTGAAGGCAAAGG+TGG | 0.580888 | 4.4:+21276906 | None:intergenic |
| TTGGGAAGTGAAGTGGCTGC+TGG | 0.584437 | 4.4:+21276759 | None:intergenic |
| TCAATTCCAAATATGCCTCA+AGG | 0.589431 | 4.4:-21276894 | MS.gene053568:CDS |
| GCTAGAAGTTGAAGGTGGTG+GGG | 0.609650 | 4.4:+21276692 | None:intergenic |
| GTAGGAAGACTTGGAAGTGG+TGG | 0.611131 | 4.4:+21276822 | None:intergenic |
| TAAGGGCTAGAAGTTGAAGG+TGG | 0.613687 | 4.4:+21276687 | None:intergenic |
| ATATTTGGAATTGAAGGCAA+AGG | 0.615183 | 4.4:+21276903 | None:intergenic |
| TAGTGAGTTTAGGCATAGAA+GGG | 0.616158 | 4.4:+21276847 | None:intergenic |
| ATAGAAGGGATAGTAGGCAA+TGG | 0.624968 | 4.4:+21276861 | None:intergenic |
| AGGAAGGTTGGGAAGTGAAG+TGG | 0.630070 | 4.4:+21276752 | None:intergenic |
| AATGGTGGAATGTTGAGAGA+TGG | 0.653205 | 4.4:+21276783 | None:intergenic |
| AATGGAGGAAGATTTCCTTG+AGG | 0.717776 | 4.4:+21276879 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| AGATGGAATTGTTGGAATAT+TGG | + | chr4.4:21276939-21276958 | None:intergenic | 30.0% | |
| ! | ATATTTGGAATTGAAGGCAA+AGG | + | chr4.4:21276836-21276855 | None:intergenic | 30.0% |
| AAGAAGTAAAGCTGCAATGA+AGG | + | chr4.4:21276705-21276724 | None:intergenic | 35.0% | |
| AGATTTCCTTGAGGCATATT+TGG | + | chr4.4:21276851-21276870 | None:intergenic | 35.0% | |
| ATAGTGAGTTTAGGCATAGA+AGG | + | chr4.4:21276893-21276912 | None:intergenic | 35.0% | |
| ATGTTGAGAGATGGAATTGT+TGG | + | chr4.4:21276947-21276966 | None:intergenic | 35.0% | |
| GGAATTGTTGGAATATTGGT+AGG | + | chr4.4:21276935-21276954 | None:intergenic | 35.0% | |
| TAAAGCTGCAATGAAGGATT+TGG | + | chr4.4:21276699-21276718 | None:intergenic | 35.0% | |
| TAGTGAGTTTAGGCATAGAA+GGG | + | chr4.4:21276892-21276911 | None:intergenic | 35.0% | |
| TCAATTCCAAATATGCCTCA+AGG | - | chr4.4:21276842-21276861 | MS.gene053568:CDS | 35.0% | |
| ! | TGAGGCATATTTGGAATTGA+AGG | + | chr4.4:21276842-21276861 | None:intergenic | 35.0% |
| !! | GTGTTGTTGTTTGCAATAGA+TGG | + | chr4.4:21276763-21276782 | None:intergenic | 35.0% |
| !!! | GTAGTTGGTTTTGGTAAAGT+GGG | + | chr4.4:21276806-21276825 | None:intergenic | 35.0% |
| !!! | TGTAGTTGGTTTTGGTAAAG+TGG | + | chr4.4:21276807-21276826 | None:intergenic | 35.0% |
| AATGGAGGAAGATTTCCTTG+AGG | + | chr4.4:21276860-21276879 | None:intergenic | 40.0% | |
| ATAGAAGGGATAGTAGGCAA+TGG | + | chr4.4:21276878-21276897 | None:intergenic | 40.0% | |
| GGAATATTGGTAGGAAGACT+TGG | + | chr4.4:21276926-21276945 | None:intergenic | 40.0% | |
| GTGGGAATGGTAGGTAAATT+TGG | + | chr4.4:21276788-21276807 | None:intergenic | 40.0% | |
| TTAGGCATAGAAGGGATAGT+AGG | + | chr4.4:21276884-21276903 | None:intergenic | 40.0% | |
| TTTGGTAAAGTGGGAATGGT+AGG | + | chr4.4:21276797-21276816 | None:intergenic | 40.0% | |
| ! | AATGGTGGAATGTTGAGAGA+TGG | + | chr4.4:21276956-21276975 | None:intergenic | 40.0% |
| ! | TGCAATGAAGGATTTGGTTG+AGG | + | chr4.4:21276693-21276712 | None:intergenic | 40.0% |
| ! | TTTGGAATTGAAGGCAAAGG+TGG | + | chr4.4:21276833-21276852 | None:intergenic | 40.0% |
| !! | AAGGTGGTGGGGAAAAAAAT+GGG | + | chr4.4:21277036-21277055 | None:intergenic | 40.0% |
| !! | GGTGGTAATGTAGTTGGTTT+TGG | + | chr4.4:21276815-21276834 | None:intergenic | 40.0% |
| !!! | TGGTTTTGGTAAAGTGGGAA+TGG | + | chr4.4:21276801-21276820 | None:intergenic | 40.0% |
| AAGTGGTTGGGATTGAAGGA+AGG | + | chr4.4:21277003-21277022 | None:intergenic | 45.0% | |
| AGTGGTGGCATAGTGAGTTT+AGG | + | chr4.4:21276902-21276921 | None:intergenic | 45.0% | |
| GGGAAAAAAATGGGAAGGAG+GGG | + | chr4.4:21277027-21277046 | None:intergenic | 45.0% | |
| GGGGAAAAAAATGGGAAGGA+GGG | + | chr4.4:21277028-21277047 | None:intergenic | 45.0% | |
| GTTGGGATTGAAGGAAGGTT+GGG | + | chr4.4:21276998-21277017 | None:intergenic | 45.0% | |
| TAAGGGCTAGAAGTTGAAGG+TGG | + | chr4.4:21277052-21277071 | None:intergenic | 45.0% | |
| TGGGGAAAAAAATGGGAAGG+AGG | + | chr4.4:21277029-21277048 | None:intergenic | 45.0% | |
| TGGTGGGGAAAAAAATGGGA+AGG | + | chr4.4:21277032-21277051 | None:intergenic | 45.0% | |
| TTGGTAGGAAGACTTGGAAG+TGG | + | chr4.4:21276920-21276939 | None:intergenic | 45.0% | |
| ! | GGCAAAGGTGGTAATGTAGT+TGG | + | chr4.4:21276821-21276840 | None:intergenic | 45.0% |
| !! | GAAGGTGGTGGGGAAAAAAA+TGG | + | chr4.4:21277037-21277056 | None:intergenic | 45.0% |
| AAATGGGAAGGAGGGGAAAG+TGG | + | chr4.4:21277020-21277039 | None:intergenic | 50.0% | |
| AGGAAGGTTGGGAAGTGAAG+TGG | + | chr4.4:21276987-21277006 | None:intergenic | 50.0% | |
| GAAGGGATAGTAGGCAATGG+AGG | + | chr4.4:21276875-21276894 | None:intergenic | 50.0% | |
| GCAATAGATGGCGAGCTTCT+AGG | + | chr4.4:21276751-21276770 | None:intergenic | 50.0% | |
| GCTAGAAGTTGAAGGTGGTG+GGG | + | chr4.4:21277047-21277066 | None:intergenic | 50.0% | |
| GGCTAGAAGTTGAAGGTGGT+GGG | + | chr4.4:21277048-21277067 | None:intergenic | 50.0% | |
| GGGAAAGTGGTTGGGATTGA+AGG | + | chr4.4:21277007-21277026 | None:intergenic | 50.0% | |
| GGTTGGGATTGAAGGAAGGT+TGG | + | chr4.4:21276999-21277018 | None:intergenic | 50.0% | |
| GTAGGAAGACTTGGAAGTGG+TGG | + | chr4.4:21276917-21276936 | None:intergenic | 50.0% | |
| AGTGAAGTGGCTGCTGGCAA+TGG | + | chr4.4:21276974-21276993 | None:intergenic | 55.0% | |
| GGAAGGAGGGGAAAGTGGTT+GGG | + | chr4.4:21277015-21277034 | None:intergenic | 55.0% | |
| GGGCTAGAAGTTGAAGGTGG+TGG | + | chr4.4:21277049-21277068 | None:intergenic | 55.0% | |
| TTGGGAAGTGAAGTGGCTGC+TGG | + | chr4.4:21276980-21276999 | None:intergenic | 55.0% | |
| GAAGTGGCTGCTGGCAATGG+TGG | + | chr4.4:21276971-21276990 | None:intergenic | 60.0% | |
| GGGAAGGAGGGGAAAGTGGT+TGG | + | chr4.4:21277016-21277035 | None:intergenic | 60.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr4.4 | gene | 21276686 | 21277072 | 21276686 | ID=MS.gene053568 |
| chr4.4 | mRNA | 21276686 | 21277072 | 21276686 | ID=MS.gene053568.t1;Parent=MS.gene053568 |
| chr4.4 | exon | 21276686 | 21277072 | 21276686 | ID=MS.gene053568.t1.exon1;Parent=MS.gene053568.t1 |
| chr4.4 | CDS | 21276686 | 21277072 | 21276686 | ID=cds.MS.gene053568.t1;Parent=MS.gene053568.t1 |
| Gene Sequence |
| Protein sequence |
