Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene80769.t1 | AAF04584.1 | 100 | 75 | 0 | 0 | 1 | 75 | 1 | 75 | 1.80E-27 | 131.3 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene80769.t1 | P43399 | 90.7 | 75 | 7 | 0 | 1 | 75 | 1 | 75 | 3.3e-25 | 115.2 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene80769.t1 | Q9SP23 | 100.0 | 75 | 0 | 0 | 1 | 75 | 1 | 75 | 1.3e-27 | 131.3 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene049424 | MS.gene80769 | 0.802351 | 5.85E-49 | -1.69E-46 |
| MS.gene053006 | MS.gene80769 | 0.804175 | 2.45E-49 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene80769.t1 | MTR_4g021725 | 94.667 | 75 | 4 | 0 | 1 | 75 | 1 | 75 | 3.47e-46 | 142 |
| MS.gene80769.t1 | MTR_4g021730 | 90.667 | 75 | 7 | 0 | 1 | 75 | 1 | 75 | 6.27e-43 | 134 |
| MS.gene80769.t1 | MTR_7g061018 | 50.725 | 69 | 31 | 2 | 8 | 75 | 12 | 78 | 6.97e-11 | 52.8 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 23 sgRNAs with CRISPR-Local
Find 94 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| AACCACAGAAACCGTGATTC+TGG | 0.293571 | 4.4:+80148170 | MS.gene80769:CDS |
| CCATTTCAGCACCCTCAAAA+TGG | 0.321140 | 4.4:-80148212 | None:intergenic |
| CGTGATTCTGGGCGTTGGTC+CGG | 0.396505 | 4.4:+80148182 | MS.gene80769:CDS |
| CCCTCAAAATGGATCTTTGC+CGG | 0.397774 | 4.4:-80148201 | None:intergenic |
| AACAGCAGGTCTAGTGGGTT+GGG | 0.444520 | 4.4:+80148132 | MS.gene80769:CDS |
| CACTTCACTTACAGTTGCAT+GGG | 0.476971 | 4.4:-80148287 | None:intergenic |
| CAACAGCAGGTCTAGTGGGT+TGG | 0.483236 | 4.4:+80148131 | MS.gene80769:CDS |
| ACCACAGAAACCGTGATTCT+GGG | 0.490783 | 4.4:+80148171 | MS.gene80769:CDS |
| AGTTGAGTTGTGAATATGTC+TGG | 0.501938 | 4.4:+80146762 | None:intergenic |
| GCACTTCACTTACAGTTGCA+TGG | 0.509517 | 4.4:-80148288 | None:intergenic |
| TGCCTTGCAGATGCAACAGC+AGG | 0.516088 | 4.4:+80148118 | MS.gene80769:intron |
| AATATGTCTGGCTGTAATTG+TGG | 0.558338 | 4.4:+80146774 | None:intergenic |
| GCCCAGAATCACGGTTTCTG+TGG | 0.562120 | 4.4:-80148172 | None:intergenic |
| GAGGATGGTGGCTGCAAGTG+TGG | 0.564753 | 4.4:+80148246 | MS.gene80769:CDS |
| GATGCAACAGCAGGTCTAGT+GGG | 0.579454 | 4.4:+80148127 | MS.gene80769:intron |
| TGAAATGGGTGTTGCAGCTG+AGG | 0.589074 | 4.4:+80148227 | MS.gene80769:CDS |
| GAAACCGTGATTCTGGGCGT+TGG | 0.589097 | 4.4:+80148177 | MS.gene80769:CDS |
| CGGACCAACGCCCAGAATCA+CGG | 0.590019 | 4.4:-80148181 | None:intergenic |
| ATGGGTGTTGCAGCTGAGGA+TGG | 0.598628 | 4.4:+80148231 | MS.gene80769:CDS |
| TGTGGAAGTAGTTGCAATTG+TGG | 0.604631 | 4.4:+80146792 | MS.gene80769:CDS |
| AGTGGGTTGGGCTATCTCGA+AGG | 0.606024 | 4.4:+80148144 | MS.gene80769:CDS |
| AGATGCAACAGCAGGTCTAG+TGG | 0.608709 | 4.4:+80148126 | MS.gene80769:intron |
| GGTGTTGCAGCTGAGGATGG+TGG | 0.628303 | 4.4:+80148234 | MS.gene80769:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | AAAGAAATTATGAAAAATTA+AGG | - | chr4.4:80147139-80147158 | None:intergenic | 10.0% |
| !! | AACAAAATATATATTATTTC+TGG | - | chr4.4:80147776-80147795 | None:intergenic | 10.0% |
| !! | AAGAAATTATGAAAAATTAA+GGG | - | chr4.4:80147138-80147157 | None:intergenic | 10.0% |
| !! | ATATATATATATATATGCAA+GGG | + | chr4.4:80147255-80147274 | MS.gene80769:intron | 10.0% |
| !! | TATATATATATATATATGCA+AGG | + | chr4.4:80147254-80147273 | MS.gene80769:intron | 10.0% |
| !! | TTAAATAAGAAAATTAGATA+AGG | + | chr4.4:80147735-80147754 | MS.gene80769:intron | 10.0% |
| !!! | TAATTTTTCATAATTTCTTT+AGG | + | chr4.4:80147139-80147158 | MS.gene80769:intron | 10.0% |
| !!! | TTCTTTAGAAAAATATATAA+GGG | - | chr4.4:80148056-80148075 | None:intergenic | 10.0% |
| !!! | TTTCTTTAGAAAAATATATA+AGG | - | chr4.4:80148057-80148076 | None:intergenic | 10.0% |
| !! | AATAGTTCTTAAATAAGAAA+CGG | - | chr4.4:80147023-80147042 | None:intergenic | 15.0% |
| !! | CTAATTAAAATATTGATATG+AGG | - | chr4.4:80147711-80147730 | None:intergenic | 15.0% |
| !! | GAAACAAAAACAAAAAAAAA+TGG | - | chr4.4:80146962-80146981 | None:intergenic | 15.0% |
| !!! | AAAGAATCCTATTTAAAATA+TGG | - | chr4.4:80147540-80147559 | None:intergenic | 15.0% |
| !!! | AAATATATATTATTTCTGGA+CGG | - | chr4.4:80147772-80147791 | None:intergenic | 15.0% |
| !!! | AGCTTTTTTTTTTTTTTTTG+TGG | - | chr4.4:80147300-80147319 | None:intergenic | 15.0% |
| !!! | GATTGAATTTCTTTTAAAAA+AGG | + | chr4.4:80147373-80147392 | MS.gene80769:intron | 15.0% |
| !!! | TCTTTAGAAAAATATATAAG+GGG | - | chr4.4:80148055-80148074 | None:intergenic | 15.0% |
| !! | AAGTTGTCAATAGAAAAAAT+AGG | + | chr4.4:80147470-80147489 | MS.gene80769:intron | 20.0% |
| !! | AATATGCAAAACATAATCTA+AGG | - | chr4.4:80147630-80147649 | None:intergenic | 20.0% |
| !! | AGTTGTCAATAGAAAAAATA+GGG | + | chr4.4:80147471-80147490 | MS.gene80769:intron | 20.0% |
| !! | TAGAACAAATAAGTAACTTT+AGG | - | chr4.4:80147606-80147625 | None:intergenic | 20.0% |
| !!! | AACTCATCCATATTTTAAAT+AGG | + | chr4.4:80147530-80147549 | MS.gene80769:intron | 20.0% |
| !!! | TCTTTTTTTAAGTAACGATT+AGG | + | chr4.4:80147865-80147884 | MS.gene80769:intron | 20.0% |
| !!! | TGTTTTTTTCATTACTCTTT+TGG | + | chr4.4:80146911-80146930 | MS.gene80769:intron | 20.0% |
| !!! | TTTTTTTTTTTTTTTGTGGC+CGG | - | chr4.4:80147296-80147315 | None:intergenic | 20.0% |
| ! | ACAATTGCAACAAAGAATTA+AGG | - | chr4.4:80146844-80146863 | None:intergenic | 25.0% |
| ! | TGGAGATTATATGATTCAAA+AGG | + | chr4.4:80147504-80147523 | MS.gene80769:intron | 25.0% |
| ! | TGTATCTTATGAATGAATCT+TGG | + | chr4.4:80146867-80146886 | MS.gene80769:intron | 25.0% |
| !!! | TTTTTTTTTTTTTTGTGGCC+GGG | - | chr4.4:80147295-80147314 | None:intergenic | 25.0% |
| ATATTCCCAAATAAACGCAA+AGG | - | chr4.4:80147941-80147960 | None:intergenic | 30.0% | |
| TATATATATATGCAAGGGTC+CGG | + | chr4.4:80147260-80147279 | MS.gene80769:intron | 30.0% | |
| TATTCCCAAATAAACGCAAA+GGG | - | chr4.4:80147940-80147959 | None:intergenic | 30.0% | |
| TCTGGATTTATCAATCTGAA+CGG | + | chr4.4:80147069-80147088 | MS.gene80769:intron | 30.0% | |
| TTCACAATTCATGTGTATGA+TGG | + | chr4.4:80148088-80148107 | MS.gene80769:intron | 30.0% | |
| ! | CATACATGAATGAGCTTTTT+GGG | + | chr4.4:80147172-80147191 | MS.gene80769:intron | 30.0% |
| ! | TCATACATGAATGAGCTTTT+TGG | + | chr4.4:80147171-80147190 | MS.gene80769:intron | 30.0% |
| !! | AGGATTCTTTTAACTGAGAA+TGG | + | chr4.4:80147550-80147569 | MS.gene80769:intron | 30.0% |
| !! | TTTTGACTAAACGAGTGTTT+TGG | + | chr4.4:80147322-80147341 | MS.gene80769:intron | 30.0% |
| !!! | TTTTGTTTTTGTTTCCGCAT+GGG | + | chr4.4:80146967-80146986 | MS.gene80769:intron | 30.0% |
| !!! | TTTTTGTTTTTGTTTCCGCA+TGG | + | chr4.4:80146966-80146985 | MS.gene80769:intron | 30.0% |
| !!! | TTTTTTTTTTTTTGTGGCCG+GGG | - | chr4.4:80147294-80147313 | None:intergenic | 30.0% |
| ATATATATATGCAAGGGTCC+GGG | + | chr4.4:80147261-80147280 | MS.gene80769:intron | 35.0% | |
| TCTCGTGAGTTTAGATTAGT+TGG | + | chr4.4:80147217-80147236 | MS.gene80769:intron | 35.0% | |
| TGCGTTTATTTGGGAATATG+TGG | + | chr4.4:80147942-80147961 | MS.gene80769:intron | 35.0% | |
| TTGATAAATCCAGAGACTTG+TGG | - | chr4.4:80147063-80147082 | None:intergenic | 35.0% | |
| TTGGGAATATGTGGATCATT+AGG | + | chr4.4:80147951-80147970 | MS.gene80769:intron | 35.0% | |
| ! | TATTGATAACCACAAGTCTC+TGG | + | chr4.4:80147051-80147070 | MS.gene80769:intron | 35.0% |
| !! | GCTATTTCTTGTAGAGTCTA+GGG | + | chr4.4:80147887-80147906 | MS.gene80769:intron | 35.0% |
| !!! | ACACTATTTTTTCTCGCATC+CGG | + | chr4.4:80147097-80147116 | MS.gene80769:intron | 35.0% |
| !!! | TTTGTTTTTGTTTCCGCATG+GGG | + | chr4.4:80146968-80146987 | MS.gene80769:intron | 35.0% |
| GATGTAACAACGAGGACAAA+AGG | - | chr4.4:80147817-80147836 | None:intergenic | 40.0% | |
| TAAGAAACGGTGTAAGAGAC+TGG | - | chr4.4:80147010-80147029 | None:intergenic | 40.0% | |
| TATATATATGCAAGGGTCCG+GGG | + | chr4.4:80147262-80147281 | MS.gene80769:intron | 40.0% | |
| TCCTCCCTTTGCGTTTATTT+GGG | + | chr4.4:80147933-80147952 | MS.gene80769:intron | 40.0% | |
| TCCTCGTTGTTACATCCAAA+AGG | + | chr4.4:80147821-80147840 | MS.gene80769:intron | 40.0% | |
| TGTGGAAGTAGTTGCAATTG+TGG | + | chr4.4:80146792-80146811 | MS.gene80769:CDS | 40.0% | |
| ! | ACCTTTTGGATGTAACAACG+AGG | - | chr4.4:80147825-80147844 | None:intergenic | 40.0% |
| ! | TAGCTTCATCGTCAACCTTT+TGG | - | chr4.4:80147839-80147858 | None:intergenic | 40.0% |
| !! | GGCTATTTCTTGTAGAGTCT+AGG | + | chr4.4:80147886-80147905 | MS.gene80769:intron | 40.0% |
| !!! | CACTATTTTTTCTCGCATCC+GGG | + | chr4.4:80147098-80147117 | MS.gene80769:intron | 40.0% |
| !!! | CATTTTGAGGGTGCTGAAAT+GGG | + | chr4.4:80148213-80148232 | MS.gene80769:CDS | 40.0% |
| AAAAATAGGGTCGTTGCGTG+TGG | + | chr4.4:80147484-80147503 | MS.gene80769:intron | 45.0% | |
| AACCACAGAAACCGTGATTC+TGG | + | chr4.4:80148170-80148189 | MS.gene80769:CDS | 45.0% | |
| ACCACAGAAACCGTGATTCT+GGG | + | chr4.4:80148171-80148190 | MS.gene80769:CDS | 45.0% | |
| CAAATAAACGCAAAGGGAGG+AGG | - | chr4.4:80147934-80147953 | None:intergenic | 45.0% | |
| CCATTTCAGCACCCTCAAAA+TGG | - | chr4.4:80148215-80148234 | None:intergenic | 45.0% | |
| CCCTCAAAATGGATCTTTGC+CGG | - | chr4.4:80148204-80148223 | None:intergenic | 45.0% | |
| CTCCTCCCTTTGCGTTTATT+TGG | + | chr4.4:80147932-80147951 | MS.gene80769:intron | 45.0% | |
| TCCCAAATAAACGCAAAGGG+AGG | - | chr4.4:80147937-80147956 | None:intergenic | 45.0% | |
| TCTACGCTTAAAACCCCATG+CGG | - | chr4.4:80146984-80147003 | None:intergenic | 45.0% | |
| TGTGGCGATAACTGCAAGTA+AGG | + | chr4.4:80146810-80146829 | MS.gene80769:intron | 45.0% | |
| ! | CCGGCAAAGATCCATTTTGA+GGG | + | chr4.4:80148201-80148220 | MS.gene80769:CDS | 45.0% |
| ! | TCCGGCAAAGATCCATTTTG+AGG | + | chr4.4:80148200-80148219 | MS.gene80769:CDS | 45.0% |
| !!! | CCATTTTGAGGGTGCTGAAA+TGG | + | chr4.4:80148212-80148231 | MS.gene80769:CDS | 45.0% |
| AGATGCAACAGCAGGTCTAG+TGG | + | chr4.4:80148126-80148145 | MS.gene80769:intron | 50.0% | |
| AGGGAGCTTATTCTCTCACC+CGG | - | chr4.4:80147119-80147138 | None:intergenic | 50.0% | |
| GATGCAACAGCAGGTCTAGT+GGG | + | chr4.4:80148127-80148146 | MS.gene80769:intron | 50.0% | |
| ! | TGAAATGGGTGTTGCAGCTG+AGG | + | chr4.4:80148227-80148246 | MS.gene80769:CDS | 50.0% |
| !! | AACAGCAGGTCTAGTGGGTT+GGG | + | chr4.4:80148132-80148151 | MS.gene80769:CDS | 50.0% |
| AACGCAAAGGGAGGAGGATG+TGG | - | chr4.4:80147928-80147947 | None:intergenic | 55.0% | |
| GAAACCGTGATTCTGGGCGT+TGG | + | chr4.4:80148177-80148196 | MS.gene80769:CDS | 55.0% | |
| GACCTGCTGTTGCATCTGCA+AGG | - | chr4.4:80148123-80148142 | None:intergenic | 55.0% | |
| GCCCAGAATCACGGTTTCTG+TGG | - | chr4.4:80148175-80148194 | None:intergenic | 55.0% | |
| TGCCTTGCAGATGCAACAGC+AGG | + | chr4.4:80148118-80148137 | MS.gene80769:intron | 55.0% | |
| ! | ATGGGTGTTGCAGCTGAGGA+TGG | + | chr4.4:80148231-80148250 | MS.gene80769:CDS | 55.0% |
| ! | CAACAGCAGGTCTAGTGGGT+TGG | + | chr4.4:80148131-80148150 | MS.gene80769:CDS | 55.0% |
| !! | AGTGGGTTGGGCTATCTCGA+AGG | + | chr4.4:80148144-80148163 | MS.gene80769:CDS | 55.0% |
| AGGGTCCGGGGTTTAAACCC+CGG | + | chr4.4:80147274-80147293 | MS.gene80769:intron | 60.0% | |
| CGGACCAACGCCCAGAATCA+CGG | - | chr4.4:80148184-80148203 | None:intergenic | 60.0% | |
| GGAGGAGGATGTGGTGACTC+TGG | - | chr4.4:80147919-80147938 | None:intergenic | 60.0% | |
| TGTGGCCGGGGTTTAAACCC+CGG | - | chr4.4:80147282-80147301 | None:intergenic | 60.0% | |
| ! | GAGGATGGTGGCTGCAAGTG+TGG | + | chr4.4:80148246-80148265 | MS.gene80769:CDS | 60.0% |
| ! | GGTGTTGCAGCTGAGGATGG+TGG | + | chr4.4:80148234-80148253 | MS.gene80769:CDS | 60.0% |
| !! | CGTGATTCTGGGCGTTGGTC+CGG | + | chr4.4:80148182-80148201 | MS.gene80769:CDS | 60.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr4.4 | gene | 80146777 | 80148305 | 80146777 | ID=MS.gene80769 |
| chr4.4 | mRNA | 80146777 | 80148305 | 80146777 | ID=MS.gene80769.t1;Parent=MS.gene80769 |
| chr4.4 | exon | 80146777 | 80146826 | 80146777 | ID=MS.gene80769.t1.exon1;Parent=MS.gene80769.t1 |
| chr4.4 | CDS | 80146777 | 80146826 | 80146777 | ID=cds.MS.gene80769.t1;Parent=MS.gene80769.t1 |
| chr4.4 | exon | 80148128 | 80148305 | 80148128 | ID=MS.gene80769.t1.exon2;Parent=MS.gene80769.t1 |
| chr4.4 | CDS | 80148128 | 80148305 | 80148128 | ID=cds.MS.gene80769.t1;Parent=MS.gene80769.t1 |
| Gene Sequence |
| Protein sequence |
