Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene81015.t1 | XP_013459409.1 | 95.1 | 1154 | 56 | 0 | 1 | 1154 | 1 | 1154 | 0.00E+00 | 2147.1 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene81015.t1 | Q9SX33 | 72.9 | 1156 | 296 | 6 | 4 | 1148 | 11 | 1160 | 0.0e+00 | 1677.5 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene81015.t1 | A0A072V5J0 | 95.1 | 1154 | 56 | 0 | 1 | 1154 | 1 | 1154 | 0.0e+00 | 2147.1 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene052681 | MS.gene81015 | 0.823186 | 1.59E-53 | -1.69E-46 |
| MS.gene052682 | MS.gene81015 | 0.817259 | 3.62E-52 | -1.69E-46 |
| MS.gene054576 | MS.gene81015 | 0.831501 | 1.61E-55 | -1.69E-46 |
| MS.gene056403 | MS.gene81015 | 0.80443 | 2.17E-49 | -1.69E-46 |
| MS.gene058289 | MS.gene81015 | 0.80889 | 2.48E-50 | -1.69E-46 |
| MS.gene059774 | MS.gene81015 | 0.827249 | 1.74E-54 | -1.69E-46 |
| MS.gene060697 | MS.gene81015 | 0.823298 | 1.49E-53 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene81015.t1 | MTR_3g037330 | 97.487 | 1154 | 29 | 0 | 1 | 1154 | 1 | 1154 | 0.0 | 2337 |
| MS.gene81015.t1 | MTR_3g037330 | 97.644 | 1061 | 25 | 0 | 94 | 1154 | 24 | 1084 | 0.0 | 2151 |
| MS.gene81015.t1 | MTR_8g069920 | 77.048 | 1172 | 248 | 5 | 2 | 1154 | 5 | 1174 | 0.0 | 1878 |
| MS.gene81015.t1 | MTR_0021s0360 | 76.883 | 1155 | 258 | 7 | 5 | 1154 | 4 | 1154 | 0.0 | 1822 |
| MS.gene81015.t1 | MTR_4g112430 | 67.563 | 1153 | 363 | 5 | 4 | 1148 | 7 | 1156 | 0.0 | 1637 |
| MS.gene81015.t1 | MTR_6g006910 | 62.511 | 1163 | 416 | 7 | 4 | 1149 | 7 | 1166 | 0.0 | 1523 |
| MS.gene81015.t1 | MTR_2g099690 | 55.161 | 1182 | 482 | 7 | 5 | 1152 | 10 | 1177 | 0.0 | 1345 |
| MS.gene81015.t1 | MTR_2g099690 | 56.641 | 911 | 345 | 8 | 5 | 880 | 10 | 905 | 0.0 | 1028 |
| MS.gene81015.t1 | MTR_5g037210 | 45.357 | 1120 | 578 | 14 | 35 | 1141 | 23 | 1121 | 0.0 | 978 |
| MS.gene81015.t1 | MTR_2g099690 | 54.680 | 876 | 347 | 8 | 5 | 845 | 10 | 870 | 0.0 | 952 |
| MS.gene81015.t1 | MTR_8g018290 | 53.874 | 839 | 342 | 5 | 349 | 1152 | 3 | 831 | 0.0 | 937 |
| MS.gene81015.t1 | MTR_8g018290 | 53.810 | 840 | 348 | 6 | 349 | 1152 | 3 | 838 | 0.0 | 934 |
| MS.gene81015.t1 | MTR_7g073480 | 37.457 | 1156 | 637 | 25 | 28 | 1134 | 135 | 1253 | 0.0 | 698 |
| MS.gene81015.t1 | MTR_3g007240 | 36.568 | 1113 | 647 | 17 | 31 | 1134 | 116 | 1178 | 0.0 | 663 |
| MS.gene81015.t1 | MTR_8g044210 | 36.757 | 1110 | 642 | 21 | 41 | 1134 | 78 | 1143 | 0.0 | 654 |
| MS.gene81015.t1 | MTR_2g090610 | 31.747 | 1093 | 639 | 28 | 45 | 1124 | 8 | 1006 | 5.69e-166 | 521 |
| MS.gene81015.t1 | MTR_8g062150 | 46.486 | 555 | 278 | 8 | 36 | 584 | 44 | 585 | 2.37e-158 | 484 |
| MS.gene81015.t1 | MTR_2g090610 | 32.533 | 999 | 571 | 25 | 45 | 1028 | 8 | 918 | 3.40e-155 | 488 |
| MS.gene81015.t1 | MTR_3g007240 | 36.385 | 841 | 485 | 15 | 31 | 861 | 116 | 916 | 9.41e-153 | 481 |
| MS.gene81015.t1 | MTR_8g027160 | 25.253 | 594 | 323 | 20 | 403 | 972 | 347 | 843 | 3.32e-22 | 103 |
| MS.gene81015.t1 | MTR_8g027160 | 25.253 | 594 | 323 | 20 | 403 | 972 | 347 | 843 | 4.01e-22 | 103 |
| MS.gene81015.t1 | MTR_2g056040 | 65.625 | 64 | 22 | 0 | 676 | 739 | 1 | 64 | 6.92e-22 | 91.3 |
| MS.gene81015.t1 | MTR_2g105830 | 25.379 | 528 | 238 | 20 | 405 | 912 | 482 | 873 | 2.13e-18 | 91.7 |
| MS.gene81015.t1 | MTR_4g008650 | 27.727 | 220 | 118 | 7 | 554 | 760 | 521 | 712 | 7.97e-13 | 73.6 |
| MS.gene81015.t1 | MTR_4g008650 | 27.727 | 220 | 118 | 7 | 554 | 760 | 521 | 712 | 1.16e-12 | 72.8 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene81015.t1 | AT1G68710 | 73.050 | 1154 | 294 | 6 | 6 | 1148 | 13 | 1160 | 0.0 | 1771 |
| MS.gene81015.t1 | AT1G68710 | 73.050 | 1154 | 294 | 6 | 6 | 1148 | 13 | 1160 | 0.0 | 1771 |
| MS.gene81015.t1 | AT1G68710 | 71.441 | 1180 | 294 | 7 | 6 | 1148 | 13 | 1186 | 0.0 | 1759 |
| MS.gene81015.t1 | AT1G26130 | 70.982 | 1151 | 324 | 5 | 4 | 1148 | 11 | 1157 | 0.0 | 1718 |
| MS.gene81015.t1 | AT1G26130 | 70.982 | 1151 | 324 | 5 | 4 | 1148 | 11 | 1157 | 0.0 | 1718 |
| MS.gene81015.t1 | AT1G26130 | 70.982 | 1151 | 324 | 5 | 4 | 1148 | 11 | 1157 | 0.0 | 1718 |
| MS.gene81015.t1 | AT1G26130 | 70.982 | 1151 | 323 | 6 | 4 | 1148 | 11 | 1156 | 0.0 | 1714 |
| MS.gene81015.t1 | AT3G25610 | 70.557 | 1148 | 328 | 8 | 6 | 1147 | 11 | 1154 | 0.0 | 1714 |
| MS.gene81015.t1 | AT1G13210 | 69.792 | 1152 | 337 | 7 | 4 | 1147 | 7 | 1155 | 0.0 | 1675 |
| MS.gene81015.t1 | AT3G27870 | 63.534 | 1149 | 395 | 8 | 4 | 1138 | 7 | 1145 | 0.0 | 1555 |
| MS.gene81015.t1 | AT3G27870 | 64.990 | 994 | 324 | 8 | 4 | 983 | 7 | 990 | 0.0 | 1360 |
| MS.gene81015.t1 | AT3G13900 | 54.291 | 1177 | 482 | 8 | 9 | 1145 | 13 | 1173 | 0.0 | 1327 |
| MS.gene81015.t1 | AT1G17500 | 54.880 | 1168 | 492 | 7 | 5 | 1152 | 9 | 1161 | 0.0 | 1325 |
| MS.gene81015.t1 | AT1G17500 | 54.880 | 1168 | 492 | 7 | 5 | 1152 | 9 | 1161 | 0.0 | 1325 |
| MS.gene81015.t1 | AT3G27870 | 63.929 | 962 | 325 | 6 | 191 | 1138 | 1 | 954 | 0.0 | 1322 |
| MS.gene81015.t1 | AT1G72700 | 53.859 | 1179 | 499 | 8 | 5 | 1152 | 9 | 1173 | 0.0 | 1314 |
| MS.gene81015.t1 | AT1G72700 | 53.859 | 1179 | 499 | 8 | 5 | 1152 | 9 | 1173 | 0.0 | 1314 |
| MS.gene81015.t1 | AT1G72700 | 53.859 | 1179 | 499 | 8 | 5 | 1152 | 9 | 1173 | 0.0 | 1314 |
| MS.gene81015.t1 | AT1G54280 | 53.571 | 1176 | 494 | 6 | 9 | 1145 | 13 | 1175 | 0.0 | 1309 |
| MS.gene81015.t1 | AT1G54280 | 53.571 | 1176 | 494 | 6 | 9 | 1145 | 13 | 1175 | 0.0 | 1309 |
| MS.gene81015.t1 | AT1G54280 | 53.571 | 1176 | 494 | 6 | 9 | 1145 | 13 | 1175 | 0.0 | 1309 |
| MS.gene81015.t1 | AT3G13900 | 56.184 | 954 | 360 | 9 | 9 | 920 | 13 | 950 | 0.0 | 1083 |
| MS.gene81015.t1 | AT3G13900 | 55.568 | 916 | 351 | 8 | 9 | 884 | 13 | 912 | 0.0 | 1023 |
| MS.gene81015.t1 | AT1G59820 | 45.013 | 1113 | 585 | 14 | 36 | 1141 | 36 | 1128 | 0.0 | 962 |
| MS.gene81015.t1 | AT5G04930 | 36.413 | 1115 | 617 | 24 | 35 | 1124 | 67 | 1114 | 0.0 | 648 |
| MS.gene81015.t1 | AT5G44240 | 32.472 | 1084 | 623 | 30 | 55 | 1124 | 18 | 1006 | 4.43e-165 | 519 |
| MS.gene81015.t1 | AT5G44240 | 32.472 | 1084 | 623 | 30 | 55 | 1124 | 50 | 1038 | 1.26e-164 | 518 |
| MS.gene81015.t1 | AT4G00900 | 25.000 | 592 | 324 | 18 | 403 | 972 | 346 | 839 | 8.12e-23 | 106 |
| MS.gene81015.t1 | AT4G00900 | 25.000 | 592 | 324 | 18 | 403 | 972 | 346 | 839 | 8.12e-23 | 106 |
| MS.gene81015.t1 | AT1G07670 | 24.831 | 592 | 302 | 22 | 403 | 966 | 361 | 837 | 5.04e-19 | 94.0 |
| MS.gene81015.t1 | AT1G07670 | 24.831 | 592 | 302 | 22 | 403 | 966 | 361 | 837 | 5.04e-19 | 94.0 |
| MS.gene81015.t1 | AT3G18700 | 54.667 | 75 | 29 | 3 | 936 | 1007 | 5 | 77 | 2.19e-13 | 70.1 |
| MS.gene81015.t1 | AT1G07810 | 25.905 | 359 | 187 | 13 | 403 | 736 | 361 | 665 | 5.49e-11 | 67.8 |
Find 0 sgRNAs with CRISPR-Local
Find 0 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| 23421 | gene | 168019 | 175102 | 168019 | ID=MS.gene81015 |
| 23421 | mRNA | 168019 | 175102 | 168019 | ID=MS.gene81015.t1;Parent=MS.gene81015 |
| 23421 | exon | 168019 | 168408 | 168019 | ID=MS.gene81015.t1.exon1;Parent=MS.gene81015.t1 |
| 23421 | CDS | 168019 | 168408 | 168019 | ID=cds.MS.gene81015.t1;Parent=MS.gene81015.t1 |
| 23421 | exon | 169258 | 170585 | 169258 | ID=MS.gene81015.t1.exon2;Parent=MS.gene81015.t1 |
| 23421 | CDS | 169258 | 170585 | 169258 | ID=cds.MS.gene81015.t1;Parent=MS.gene81015.t1 |
| 23421 | exon | 170763 | 170873 | 170763 | ID=MS.gene81015.t1.exon3;Parent=MS.gene81015.t1 |
| 23421 | CDS | 170763 | 170873 | 170763 | ID=cds.MS.gene81015.t1;Parent=MS.gene81015.t1 |
| 23421 | exon | 170968 | 171244 | 170968 | ID=MS.gene81015.t1.exon4;Parent=MS.gene81015.t1 |
| 23421 | CDS | 170968 | 171244 | 170968 | ID=cds.MS.gene81015.t1;Parent=MS.gene81015.t1 |
| 23421 | exon | 171338 | 171426 | 171338 | ID=MS.gene81015.t1.exon5;Parent=MS.gene81015.t1 |
| 23421 | CDS | 171338 | 171426 | 171338 | ID=cds.MS.gene81015.t1;Parent=MS.gene81015.t1 |
| 23421 | exon | 171523 | 171634 | 171523 | ID=MS.gene81015.t1.exon6;Parent=MS.gene81015.t1 |
| 23421 | CDS | 171523 | 171634 | 171523 | ID=cds.MS.gene81015.t1;Parent=MS.gene81015.t1 |
| 23421 | exon | 172385 | 172597 | 172385 | ID=MS.gene81015.t1.exon7;Parent=MS.gene81015.t1 |
| 23421 | CDS | 172385 | 172597 | 172385 | ID=cds.MS.gene81015.t1;Parent=MS.gene81015.t1 |
| 23421 | exon | 172906 | 173031 | 172906 | ID=MS.gene81015.t1.exon8;Parent=MS.gene81015.t1 |
| 23421 | CDS | 172906 | 173031 | 172906 | ID=cds.MS.gene81015.t1;Parent=MS.gene81015.t1 |
| 23421 | exon | 173144 | 173242 | 173144 | ID=MS.gene81015.t1.exon9;Parent=MS.gene81015.t1 |
| 23421 | CDS | 173144 | 173242 | 173144 | ID=cds.MS.gene81015.t1;Parent=MS.gene81015.t1 |
| 23421 | exon | 173595 | 173786 | 173595 | ID=MS.gene81015.t1.exon10;Parent=MS.gene81015.t1 |
| 23421 | CDS | 173595 | 173786 | 173595 | ID=cds.MS.gene81015.t1;Parent=MS.gene81015.t1 |
| 23421 | exon | 174575 | 175102 | 174575 | ID=MS.gene81015.t1.exon11;Parent=MS.gene81015.t1 |
| 23421 | CDS | 174575 | 175102 | 174575 | ID=cds.MS.gene81015.t1;Parent=MS.gene81015.t1 |
| Gene Sequence |
| Protein sequence |
