AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene81576


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene81576.t1 MTR_5g086230 70.149 201 54 2 5 199 1 201 5.31e-103 295
MS.gene81576.t1 MTR_5g086340 67.539 191 60 1 1 189 1 191 1.15e-92 278
MS.gene81576.t1 MTR_5g087570 74.775 111 27 1 5 115 1 110 6.97e-56 173
MS.gene81576.t1 MTR_5g086300 49.206 189 35 2 1 189 1 128 2.58e-50 162
MS.gene81576.t1 MTR_8g042830 47.283 184 50 2 4 187 13 149 2.09e-49 166
MS.gene81576.t1 MTR_5g016570 35.882 170 97 4 24 187 93 256 4.26e-29 113
MS.gene81576.t1 MTR_8g005235 32.353 170 104 6 25 190 20 182 4.64e-19 84.3
MS.gene81576.t1 MTR_3g466740 31.720 186 118 5 9 188 5 187 2.56e-18 82.4
MS.gene81576.t1 MTR_3g079390 30.052 193 115 6 1 184 4 185 3.49e-18 82.0
MS.gene81576.t1 MTR_2g041180 30.366 191 97 6 7 184 30 197 4.60e-18 81.6
MS.gene81576.t1 MTR_8g005285 30.601 183 99 5 25 184 29 206 3.48e-17 79.3
MS.gene81576.t1 MTR_2g036320 32.418 182 105 6 7 184 47 214 4.43e-17 79.0
MS.gene81576.t1 MTR_0209s0060 33.728 169 99 8 18 181 16 176 2.73e-16 76.6
MS.gene81576.t1 MTR_0209s0030 33.728 169 99 8 18 181 16 176 2.73e-16 76.6
MS.gene81576.t1 MTR_7g067940 32.000 175 110 5 13 184 12 180 3.05e-16 76.6
MS.gene81576.t1 MTR_0067s0010 30.052 193 110 7 5 187 22 199 3.90e-16 76.3
MS.gene81576.t1 MTR_7g068070 33.758 157 95 5 33 183 30 183 8.59e-16 75.1
MS.gene81576.t1 MTR_8g005225 33.523 176 98 7 11 183 25 184 2.64e-15 73.9
MS.gene81576.t1 MTR_8g005265 34.615 156 91 4 30 181 25 173 2.70e-15 73.6
MS.gene81576.t1 MTR_8g006525 30.909 165 105 5 29 190 25 183 3.44e-15 73.2
MS.gene81576.t1 MTR_8g005325 32.919 161 97 6 25 181 49 202 4.03e-15 73.2
MS.gene81576.t1 MTR_8g005295 30.233 172 92 5 33 181 37 203 5.01e-15 73.2
MS.gene81576.t1 MTR_0024s0080 28.859 149 94 3 30 178 23 159 6.36e-15 72.4
MS.gene81576.t1 MTR_6g011060 30.137 146 90 3 33 178 26 159 2.73e-14 70.9
MS.gene81576.t1 MTR_6g009170 33.645 107 60 2 33 135 26 125 8.94e-14 69.3
MS.gene81576.t1 MTR_7g068160 33.333 165 90 7 33 184 37 194 1.30e-13 68.9
MS.gene81576.t1 MTR_6g011050 28.188 149 97 3 30 178 22 160 3.43e-13 67.4
MS.gene81576.t1 MTR_4g009620 28.125 160 105 4 33 185 43 199 9.05e-13 66.6
MS.gene81576.t1 MTR_6g010890 28.378 148 96 4 33 178 26 165 9.98e-13 66.2
MS.gene81576.t1 MTR_4g009590 26.667 165 110 4 33 189 43 204 1.56e-12 65.9
MS.gene81576.t1 MTR_7g067660 30.435 161 98 7 33 181 17 175 2.36e-12 65.1
MS.gene81576.t1 MTR_7g067750 31.056 161 99 6 33 184 64 221 2.67e-12 65.1
MS.gene81576.t1 MTR_6g009590 23.784 185 129 2 15 199 8 180 3.22e-12 64.7
MS.gene81576.t1 MTR_6g010920 25.455 165 111 3 30 194 23 175 3.60e-12 64.7
MS.gene81576.t1 MTR_7g067880 29.268 164 98 7 30 181 27 184 5.30e-12 64.3
MS.gene81576.t1 MTR_4g009340 28.302 159 103 4 33 183 43 198 6.57e-12 63.9
MS.gene81576.t1 MTR_6g009610 25.325 154 103 3 25 178 18 159 2.04e-11 62.4
MS.gene81576.t1 MTR_7g017620 26.415 159 106 3 33 183 42 197 5.78e-11 61.2
MS.gene81576.t1 MTR_8g042080 30.263 152 87 6 34 174 26 169 8.62e-11 58.5
MS.gene81576.t1 MTR_6g010810 28.221 163 103 5 18 178 8 158 8.92e-11 60.5
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene81576.t1 AT3G50170 40.588 170 91 3 24 189 105 268 4.52e-39 139
MS.gene81576.t1 AT3G50170 40.588 170 91 3 24 189 105 268 1.10e-38 139
MS.gene81576.t1 AT3G50140 34.759 187 107 4 7 189 93 268 7.25e-37 132
MS.gene81576.t1 AT3G50150 39.645 169 93 3 24 188 82 245 1.35e-36 132
MS.gene81576.t1 AT3G50150 39.645 169 93 3 24 188 82 245 2.27e-36 132
MS.gene81576.t1 AT3G50140 38.182 165 92 3 29 189 110 268 2.79e-36 133
MS.gene81576.t1 AT3G50120 38.235 170 95 3 24 189 95 258 5.78e-36 132
MS.gene81576.t1 AT3G50130 38.095 168 94 3 24 187 131 292 4.72e-35 130
MS.gene81576.t1 AT3G50180 40.588 170 91 4 24 189 170 333 5.89e-35 129
MS.gene81576.t1 AT3G50190 34.682 173 98 4 7 175 76 237 1.30e-32 120
MS.gene81576.t1 AT3G50190 34.682 173 98 4 7 175 76 237 1.54e-32 120
MS.gene81576.t1 AT3G50190 34.682 173 98 4 7 175 76 237 1.54e-32 120
MS.gene81576.t1 AT3G50160 35.484 186 101 5 7 185 82 255 2.00e-32 122
MS.gene81576.t1 AT3G50190 34.682 173 98 4 7 175 76 237 5.36e-32 120
MS.gene81576.t1 AT3G47250 37.255 153 85 5 34 181 63 209 1.25e-26 105
MS.gene81576.t1 AT3G47250 37.255 153 85 5 34 181 63 209 1.25e-26 105
MS.gene81576.t1 AT3G47250 37.255 153 85 5 34 181 63 209 1.25e-26 105
MS.gene81576.t1 AT3G47210 33.714 175 108 4 9 181 70 238 3.71e-25 102
MS.gene81576.t1 AT2G36430 36.250 160 88 4 33 187 46 196 2.32e-24 99.4
MS.gene81576.t1 AT5G22550 36.250 160 89 6 33 185 45 198 6.69e-24 98.6
MS.gene81576.t1 AT3G44710 38.028 142 76 6 46 181 62 197 9.59e-24 98.2
MS.gene81576.t1 AT5G22540 32.461 191 103 6 10 185 4 183 2.76e-23 96.3
MS.gene81576.t1 AT4G31980 32.353 170 103 5 31 194 292 455 6.47e-23 95.9
MS.gene81576.t1 AT3G47200 36.709 158 94 4 25 181 39 191 3.16e-22 93.6
MS.gene81576.t1 AT3G47200 36.709 158 94 4 25 181 39 191 3.16e-22 93.6
MS.gene81576.t1 AT3G44700 35.878 131 76 5 30 152 46 176 2.38e-19 82.4
MS.gene81576.t1 AT5G11290 30.409 171 109 4 9 178 13 174 7.39e-18 80.9
MS.gene81576.t1 AT5G22560 29.825 171 95 6 33 184 48 212 7.22e-15 72.8

Find 34 sgRNAs with CRISPR-Local

Find 42 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CCCTTCTTCATTTCCTAAAA+AGG 0.187688 5.1:+68261776 MS.gene81576:CDS
GATCCTGTCTTTAGTTTATA+TGG 0.197350 5.1:+68261988 MS.gene81576:CDS
ATAAGTCATTTGATTCGATT+TGG 0.291445 5.1:+68261866 MS.gene81576:CDS
TGGCACTGAGGTAATATTAA+TGG 0.314484 5.1:+68262107 MS.gene81576:CDS
GGTTCTTCATACGTTGATTC+AGG 0.318291 5.1:+68262077 MS.gene81576:CDS
AGGTCCAAATGAAATTGTTC+GGG 0.346218 5.1:-68261701 None:intergenic
AAACCCCGAACAATTTCATT+TGG 0.363920 5.1:+68261697 MS.gene81576:CDS
ACATGGAGTTAGAAATGAAT+TGG 0.393412 5.1:+68261548 None:intergenic
ATTCAAATGATGATTATTGA+TGG 0.405805 5.1:+68261910 MS.gene81576:CDS
TGAAATTGTTCGGGGTTTGT+AGG 0.409252 5.1:-68261692 None:intergenic
AAGGTCCAAATGAAATTGTT+CGG 0.418389 5.1:-68261702 None:intergenic
TTGGTTTGATCAAATCAATA+AGG 0.423164 5.1:+68261567 MS.gene81576:CDS
CGATGCTTGTGCTCTTCCAT+AGG 0.436817 5.1:-68261751 None:intergenic
TCATTTGGACCTTACCATCA+TGG 0.460295 5.1:+68261712 MS.gene81576:CDS
ACGTTGATTCAGGTTCAAAC+TGG 0.460312 5.1:+68262087 MS.gene81576:CDS
GGAGGACAATTTAAAGCCTA+TGG 0.467185 5.1:+68261735 MS.gene81576:CDS
CATTTGGACCTTACCATCAT+GGG 0.492283 5.1:+68261713 MS.gene81576:CDS
TTTCCATATAAACTAAAGAC+AGG 0.505866 5.1:-68261991 None:intergenic
TTAAATTGTCCTCCCCATGA+TGG 0.525645 5.1:-68261726 None:intergenic
AACGTATGAAGAACCGTCAT+AGG 0.532012 5.1:-68262069 None:intergenic
AGCTGATGAGAAGAAGCAAT+GGG 0.537660 5.1:+68261615 MS.gene81576:CDS
TTGTCCTCCCCATGATGGTA+AGG 0.541423 5.1:-68261721 None:intergenic
TTCAACTCCGTGACTTGTGA+GGG 0.542571 5.1:-68261661 None:intergenic
GTTCAACTCCGTGACTTGTG+AGG 0.545369 5.1:-68261662 None:intergenic
TCCTCTTGACTATGATCAGA+AGG 0.545995 5.1:+68261966 MS.gene81576:CDS
CAAAGTACCCTCACAAGTCA+CGG 0.580405 5.1:+68261654 MS.gene81576:CDS
TAGCTGATGAGAAGAAGCAA+TGG 0.608374 5.1:+68261614 MS.gene81576:CDS
TCCTTCTGATCATAGTCAAG+AGG 0.620363 5.1:-68261967 None:intergenic
ATTTGGACCTTACCATCATG+GGG 0.624331 5.1:+68261714 MS.gene81576:CDS
TGAGAATCAGATACCTATGA+CGG 0.632242 5.1:+68262056 MS.gene81576:CDS
GGTCCAAATGAAATTGTTCG+GGG 0.644074 5.1:-68261700 None:intergenic
TTTGATTCGATTTGGACACA+TGG 0.660066 5.1:+68261874 MS.gene81576:CDS
TGGACCTTACCATCATGGGG+AGG 0.685510 5.1:+68261717 MS.gene81576:CDS
TCAGGTTCAAACTGGCACTG+AGG 0.722569 5.1:+68262095 MS.gene81576:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! ATTCAAATGATGATTATTGA+TGG + chr5.1:68261910-68261929 MS.gene81576:CDS 20.0%
!! TGAATTGATATTCCAAAAAT+TGG + chr5.1:68261813-68261832 MS.gene81576:CDS 20.0%
!!! TTTTGGAATATCAATTCAAT+GGG - chr5.1:68261811-68261830 None:intergenic 20.0%
!!! TTTTTGGAATATCAATTCAA+TGG - chr5.1:68261812-68261831 None:intergenic 20.0%
! TTTCCATATAAACTAAAGAC+AGG - chr5.1:68261994-68262013 None:intergenic 25.0%
!! ATAAGTCATTTGATTCGATT+TGG + chr5.1:68261866-68261885 MS.gene81576:CDS 25.0%
!! TTGGTTTGATCAAATCAATA+AGG + chr5.1:68261567-68261586 MS.gene81576:CDS 25.0%
AAGGTCCAAATGAAATTGTT+CGG - chr5.1:68261705-68261724 None:intergenic 30.0%
ATTCCAAAAATTGGATCAAG+TGG + chr5.1:68261822-68261841 MS.gene81576:CDS 30.0%
! GATCCTGTCTTTAGTTTATA+TGG + chr5.1:68261988-68262007 MS.gene81576:CDS 30.0%
!! ACCTTTTTAGGAAATGAAGA+AGG - chr5.1:68261780-68261799 None:intergenic 30.0%
!! CCTTTTTAGGAAATGAAGAA+GGG - chr5.1:68261779-68261798 None:intergenic 30.0%
AAACCCCGAACAATTTCATT+TGG + chr5.1:68261697-68261716 MS.gene81576:CDS 35.0%
AGGTCCAAATGAAATTGTTC+GGG - chr5.1:68261704-68261723 None:intergenic 35.0%
CCCTTCTTCATTTCCTAAAA+AGG + chr5.1:68261776-68261795 MS.gene81576:CDS 35.0%
CTTCATTTCCTAAAAAGGTG+TGG + chr5.1:68261781-68261800 MS.gene81576:CDS 35.0%
TGAGAATCAGATACCTATGA+CGG + chr5.1:68262056-68262075 MS.gene81576:CDS 35.0%
! TGGCACTGAGGTAATATTAA+TGG + chr5.1:68262107-68262126 MS.gene81576:CDS 35.0%
!! TTTGATTCGATTTGGACACA+TGG + chr5.1:68261874-68261893 MS.gene81576:CDS 35.0%
AACGTATGAAGAACCGTCAT+AGG - chr5.1:68262072-68262091 None:intergenic 40.0%
AGCTGATGAGAAGAAGCAAT+GGG + chr5.1:68261615-68261634 MS.gene81576:CDS 40.0%
ATTTGGACCTTACCATCATG+GGG + chr5.1:68261714-68261733 MS.gene81576:CDS 40.0%
CATTTGGACCTTACCATCAT+GGG + chr5.1:68261713-68261732 MS.gene81576:CDS 40.0%
GGTCCAAATGAAATTGTTCG+GGG - chr5.1:68261703-68261722 None:intergenic 40.0%
TAGCTGATGAGAAGAAGCAA+TGG + chr5.1:68261614-68261633 MS.gene81576:CDS 40.0%
TCATTTGGACCTTACCATCA+TGG + chr5.1:68261712-68261731 MS.gene81576:CDS 40.0%
TCCTCTTGACTATGATCAGA+AGG + chr5.1:68261966-68261985 MS.gene81576:CDS 40.0%
TCCTTCTGATCATAGTCAAG+AGG - chr5.1:68261970-68261989 None:intergenic 40.0%
TGAAATTGTTCGGGGTTTGT+AGG - chr5.1:68261695-68261714 None:intergenic 40.0%
TTAAATTGTCCTCCCCATGA+TGG - chr5.1:68261729-68261748 None:intergenic 40.0%
! GAGCCACTTGATCCAATTTT+TGG - chr5.1:68261828-68261847 None:intergenic 40.0%
! GGAGGACAATTTAAAGCCTA+TGG + chr5.1:68261735-68261754 MS.gene81576:CDS 40.0%
! TGGGTTTTCCACACCTTTTT+AGG - chr5.1:68261792-68261811 None:intergenic 40.0%
!! ACGTTGATTCAGGTTCAAAC+TGG + chr5.1:68262087-68262106 MS.gene81576:CDS 40.0%
!! GGTTCTTCATACGTTGATTC+AGG + chr5.1:68262077-68262096 MS.gene81576:CDS 40.0%
! CAAAGTACCCTCACAAGTCA+CGG + chr5.1:68261654-68261673 MS.gene81576:CDS 45.0%
! TTCAACTCCGTGACTTGTGA+GGG - chr5.1:68261664-68261683 None:intergenic 45.0%
CGATGCTTGTGCTCTTCCAT+AGG - chr5.1:68261754-68261773 None:intergenic 50.0%
TCAGGTTCAAACTGGCACTG+AGG + chr5.1:68262095-68262114 MS.gene81576:CDS 50.0%
TTGTCCTCCCCATGATGGTA+AGG - chr5.1:68261724-68261743 None:intergenic 50.0%
! GTTCAACTCCGTGACTTGTG+AGG - chr5.1:68261665-68261684 None:intergenic 50.0%
TGGACCTTACCATCATGGGG+AGG + chr5.1:68261717-68261736 MS.gene81576:CDS 55.0%


Chromosome Type Strat End Strand Name
chr5.1 gene 68261550 68262149 68261550 ID=MS.gene81576
chr5.1 mRNA 68261550 68262149 68261550 ID=MS.gene81576.t1;Parent=MS.gene81576
chr5.1 exon 68261550 68262149 68261550 ID=MS.gene81576.t1.exon1;Parent=MS.gene81576.t1
chr5.1 CDS 68261550 68262149 68261550 ID=cds.MS.gene81576.t1;Parent=MS.gene81576.t1
Gene Sequence

>MS.gene81576

ATGGAGTTAGAAATGAATTGGTTTGATCAAATCAATAAGGAGATCAAGAGCAATGCTTCTTCAATAGCTGATGAGAAGAAGCAATGGGAGAAGCATTCAATTTACAAAGTACCCTCACAAGTCACGGAGTTGAACAAAAAAGCCTACAAACCCCGAACAATTTCATTTGGACCTTACCATCATGGGGAGGACAATTTAAAGCCTATGGAAGAGCACAAGCATCGAGCCCTTCTTCATTTCCTAAAAAGGTGTGGAAAACCCATTGAATTGATATTCCAAAAATTGGATCAAGTGGCTCAAGAGTTGAAAGACTCATATAAGTCATTTGATTCGATTTGGACACATGGCAGATCAAAGTTCATTCAAATGATGATTATTGATGGTTGTTTTATTCTAGAATTTTTGAAAGTCAGTACTCCTCTTGACTATGATCAGAAGGATCCTGTCTTTAGTTTATATGGAAAATTTTATATGATGCAATATATCGAACGTGACATGCTTATGCTTGAGAATCAGATACCTATGACGGTTCTTCATACGTTGATTCAGGTTCAAACTGGCACTGAGGTAATATTAATGGTTACTTTTAGCTTAATCTAG

Protein sequence

>MS.gene81576.t1

MELEMNWFDQINKEIKSNASSIADEKKQWEKHSIYKVPSQVTELNKKAYKPRTISFGPYHHGEDNLKPMEEHKHRALLHFLKRCGKPIELIFQKLDQVAQELKDSYKSFDSIWTHGRSKFIQMMIIDGCFILEFLKVSTPLDYDQKDPVFSLYGKFYMMQYIERDMLMLENQIPMTVLHTLIQVQTGTEVILMVTFSLI