AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene82336

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene82336.t1 MTR_4g082550 87.941 340 33 4 17 349 1 339 0.0 518
MS.gene82336.t1 MTR_4g082360 34.641 306 132 9 27 315 4 258 7.47e-38 137
MS.gene82336.t1 MTR_4g082365 33.564 289 130 9 27 310 4 235 1.32e-28 112
MS.gene82336.t1 MTR_4g082355 31.833 311 149 9 27 320 4 268 1.62e-27 109
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene82336.t1 AT3G11810 44.477 344 157 8 27 348 14 345 3.85e-80 248
MS.gene82336.t1 AT2G03330 52.817 142 62 2 212 348 241 382 5.84e-35 132
MS.gene82336.t1 AT5G44860 26.603 312 176 10 26 317 11 289 5.66e-11 63.2
MS.gene82336.t1 AT5G44860 26.603 312 176 10 26 317 11 289 5.66e-11 63.2

Find 127 sgRNAs with CRISPR-Local

Find 147 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
AGCGTCTTCCACGGCTTCTT+TGG 0.232115 4.2:-27133298 MS.gene82336:CDS
AGAAGGAGATCAGGCGGAGG+AGG 0.339536 4.2:+27133161 None:intergenic
GCTAGAGTTGTTTCTGATAG+TGG 0.354480 4.2:-27132899 MS.gene82336:CDS
AAGGAGATCAGGCGGAGGAG+GGG 0.362890 4.2:+27133163 None:intergenic
CGGGGAGGGAGATGGAGAAG+AGG 0.364863 4.2:+27133188 None:intergenic
TAGTGGTTCATGGAGGGATT+GGG 0.370675 4.2:-27132882 MS.gene82336:CDS
AGGAGATCAGGCGGAGGAGG+GGG 0.377880 4.2:+27133164 None:intergenic
GAGGATGAGGTGGGAGAGGA+TGG 0.378022 4.2:+27133207 None:intergenic
TTGTTCTTTGCTTCTTTGGA+AGG 0.386772 4.2:-27132950 MS.gene82336:CDS
TGTGGTGAGGAGAGGAAGAA+AGG 0.406581 4.2:+27133231 None:intergenic
GCGGAGACGGAGCGAATGAT+TGG 0.410295 4.2:+27133133 None:intergenic
TTGTTTCTGATAGTGGTTCA+TGG 0.410409 4.2:-27132892 MS.gene82336:CDS
ATAGTGGTTCATGGAGGGAT+TGG 0.411097 4.2:-27132883 MS.gene82336:CDS
GTGAGCGTTGATGATTCGTT+TGG 0.414125 4.2:+27133597 None:intergenic
AGGAGAGAGTCCATGATATA+CGG 0.414426 4.2:+27133071 None:intergenic
TGATGGTGGCGAGGGAGGAT+AGG 0.428750 4.2:+27133326 None:intergenic
CTGAGGAGGTGGAGGATGGT+AGG 0.434298 4.2:+27133508 None:intergenic
GATGGTGGCGAGGGAGGATA+GGG 0.439050 4.2:+27133327 None:intergenic
GAAGGAGATCAGGCGGAGGA+GGG 0.442662 4.2:+27133162 None:intergenic
GAACAAAAGGCAAGAAGCTC+CGG 0.446232 4.2:+27132967 None:intergenic
GAGAGAAAGAACAAGAAGAT+AGG 0.446867 4.2:+27133349 None:intergenic
CATGGTTGCAGCTGTCTTGT+TGG 0.454653 4.2:+27133672 None:intergenic
AAGAAGGATGTGGATGGTGG+GGG 0.454970 4.2:+27133471 None:intergenic
GAAGGAAGAGTGCAAGGAGG+AGG 0.455096 4.2:+27133107 None:intergenic
GGTGCTGAGGAGGTGGAGGA+TGG 0.458640 4.2:+27133504 None:intergenic
AGAAGAGGATGAGGTGGGAG+AGG 0.464084 4.2:+27133203 None:intergenic
CCAAAGAAGCAAAGAACAAA+AGG 0.464801 4.2:+27132954 None:intergenic
GGGAGATGGAGAAGAGGATG+AGG 0.470677 4.2:+27133194 None:intergenic
CGAAGAAGGATGTGGATGGT+GGG 0.472489 4.2:+27133469 None:intergenic
AGATGGAGAAGAGGATGAGG+TGG 0.473426 4.2:+27133197 None:intergenic
GATGGAGAAGAGGATGAGGT+GGG 0.473790 4.2:+27133198 None:intergenic
CAACAGCAAGCCTGAACCTA+TGG 0.478339 4.2:-27133636 MS.gene82336:CDS
CGCTGTGGGTGATGGTGGCG+AGG 0.480260 4.2:+27133317 None:intergenic
TTGGAGAGGAGAAGGAGATC+AGG 0.481727 4.2:+27133152 None:intergenic
GAGAGGGAGGAGGAAGATGA+CGG 0.483828 4.2:+27133552 None:intergenic
GGGAGAAAGAGAGAGGGAGG+AGG 0.490367 4.2:+27133542 None:intergenic
TGAGAGAGGTGACGGTGGAG+AGG 0.492052 4.2:+27133260 None:intergenic
GGAGGAGATGAGAGAGGTGA+CGG 0.492196 4.2:+27133252 None:intergenic
TCGTTTGGATTCAGAGAGGA+TGG 0.493498 4.2:+27133612 None:intergenic
AGATGAGAAGGAAGAGTGCA+AGG 0.494154 4.2:+27133101 None:intergenic
GGAGATGAGAGAGGTGACGG+TGG 0.494355 4.2:+27133255 None:intergenic
CAACAAGACAGCTGCAACCA+TGG 0.496998 4.2:-27133671 MS.gene82336:CDS
AGAGGATGGACCATAGGTTC+AGG 0.501006 4.2:+27133626 None:intergenic
AGGCAACAAGAGAAGTGGTA+AGG 0.502728 4.2:+27133369 None:intergenic
GTGGGTGATGGTGGCGAGGG+AGG 0.503105 4.2:+27133321 None:intergenic
GAGACGTTGCTTGACGAAGA+AGG 0.504538 4.2:+27133455 None:intergenic
TCTTCTACATCCGTATATCA+TGG 0.508916 4.2:-27133081 MS.gene82336:CDS
TGAGGGAGAAAGAGAGAGGG+AGG 0.509045 4.2:+27133539 None:intergenic
TTCTGATAGTGGTTCATGGA+GGG 0.509452 4.2:-27132888 MS.gene82336:CDS
ACGATGAGGGAGAAAGAGAG+AGG 0.515052 4.2:+27133535 None:intergenic
GGATGGTAGGAGAGACGATG+AGG 0.515619 4.2:+27133521 None:intergenic
AAGGAGGAGGATGGCGGAGA+CGG 0.516774 4.2:+27133120 None:intergenic
AGGATGGTTGTGGTGAGGAG+AGG 0.518553 4.2:+27133223 None:intergenic
GGTTGCAGCTGTCTTGTTGG+TGG 0.518704 4.2:+27133675 None:intergenic
GAAGGAGAGATATTTGATGA+TGG 0.518871 4.2:+27133697 None:intergenic
GATGGTAGGAGAGACGATGA+GGG 0.526665 4.2:+27133522 None:intergenic
ACGGTGGAGAGGAGTTTGAC+AGG 0.527995 4.2:+27133271 None:intergenic
CGATGAGGGAGAAAGAGAGA+GGG 0.530949 4.2:+27133536 None:intergenic
TACGGATGTAGAAGATGAGA+AGG 0.531439 4.2:+27133089 None:intergenic
GTGGAAGACGCTGTGGGTGA+TGG 0.532274 4.2:+27133309 None:intergenic
GCAGCTGTCTTGTTGGTGGA+AGG 0.532997 4.2:+27133679 None:intergenic
TAAGGCTGTACATGGTGAAC+TGG 0.533194 4.2:-27132768 MS.gene82336:CDS
GCTGTGGGTGATGGTGGCGA+GGG 0.533992 4.2:+27133318 None:intergenic
CGGAAAGAGCAAGGAAGTGA+CGG 0.534868 4.2:+27133572 None:intergenic
GGAACTTTGCCATCATCAAA+AGG 0.535133 4.2:+27132701 None:intergenic
TGAACTGGCGGAGGAGATTG+TGG 0.538035 4.2:-27132753 MS.gene82336:CDS
GCGAATGATTGGAGAGGAGA+AGG 0.541064 4.2:+27133144 None:intergenic
GAAGAAGGATGTGGATGGTG+GGG 0.541741 4.2:+27133470 None:intergenic
GAACACTCCGGCGAAGTGGT+TGG 0.543138 4.2:+27133014 None:intergenic
AGGAGAAGGAGATCAGGCGG+AGG 0.545092 4.2:+27133158 None:intergenic
CAACAAGAGAAGTGGTAAGG+GGG 0.545969 4.2:+27133372 None:intergenic
ATCAAGAGAAGTGGTAAACC+TGG 0.548604 4.2:+27133402 None:intergenic
GGCAACAAGAGAAGTGGTAA+GGG 0.557394 4.2:+27133370 None:intergenic
AAGAAAGGAGGAGATGAGAG+AGG 0.557883 4.2:+27133246 None:intergenic
CTCCTCAGTCATCGCCGTAG+TGG 0.558626 4.2:-27133053 MS.gene82336:CDS
TTGCTTGACGAAGAAGGATG+TGG 0.564705 4.2:+27133461 None:intergenic
ACGAAGAAGGATGTGGATGG+TGG 0.564723 4.2:+27133468 None:intergenic
GGTGAGGAGAGGAAGAAAGG+AGG 0.567087 4.2:+27133234 None:intergenic
CTTTGGAAGGAATTATGTTG+TGG 0.567519 4.2:-27132937 MS.gene82336:CDS
ATTCAGAGAGGATGGACCAT+AGG 0.573991 4.2:+27133620 None:intergenic
GGGAGAGGATGGTTGTGGTG+AGG 0.574534 4.2:+27133218 None:intergenic
GAAGAGTGCAAGGAGGAGGA+TGG 0.575363 4.2:+27133111 None:intergenic
GGAAGATGACGGAAAGAGCA+AGG 0.576746 4.2:+27133563 None:intergenic
GAGGTGGGAGAGGATGGTTG+TGG 0.577743 4.2:+27133213 None:intergenic
GGAAAGAGCAAGGAAGTGAC+GGG 0.578063 4.2:+27133573 None:intergenic
AAGATAGGCAACAAGAGAAG+TGG 0.582711 4.2:+27133364 None:intergenic
TGTTGATGTAGTTGTCGCCA+TGG 0.587791 4.2:+27133654 None:intergenic
ACGGAATCCAACCACTTCGC+CGG 0.587915 4.2:-27133021 MS.gene82336:CDS
CGTGCTGTATATGTACTGTA+AGG 0.602621 4.2:-27132786 MS.gene82336:CDS
AGAAGCCGTGGAAGACGCTG+TGG 0.603610 4.2:+27133302 None:intergenic
TGTACATGGTGAACTGGCGG+AGG 0.604228 4.2:-27132762 MS.gene82336:CDS
ATCACCCACAGCGTCTTCCA+CGG 0.606523 4.2:-27133307 MS.gene82336:CDS
GAAGACGCTGTGGGTGATGG+TGG 0.611015 4.2:+27133312 None:intergenic
GCAACAAGAGAAGTGGTAAG+GGG 0.611263 4.2:+27133371 None:intergenic
TGAGAAGGAAGAGTGCAAGG+AGG 0.611861 4.2:+27133104 None:intergenic
AACAAGAGAAGTGGTAAGGG+GGG 0.613965 4.2:+27133373 None:intergenic
GAGAGGAGAAGGAGATCAGG+CGG 0.614950 4.2:+27133155 None:intergenic
AAGCATCAACACTGTCATCA+CGG 0.615136 4.2:+27132829 None:intergenic
AGTTGCACACAGAAGATACC+AGG 0.615489 4.2:-27133420 MS.gene82336:CDS
TTTCTGATAGTGGTTCATGG+AGG 0.616797 4.2:-27132889 MS.gene82336:CDS
GGGAGGAAAATCAAGAGAAG+TGG 0.617081 4.2:+27133393 None:intergenic
CAGGACGGCCAAAGAAGCCG+TGG 0.617422 4.2:+27133290 None:intergenic
ATGTACTGTAAGGCTGTACA+TGG 0.618850 4.2:-27132776 MS.gene82336:CDS
GAGTGCAAGGAGGAGGATGG+CGG 0.619025 4.2:+27133114 None:intergenic
GGCTGTACATGGTGAACTGG+CGG 0.620056 4.2:-27132765 MS.gene82336:CDS
AGATTGTGGAGGAGTTTGCA+TGG 0.621100 4.2:-27132739 MS.gene82336:CDS
GAAGGAATTATGTTGTGGAG+TGG 0.623006 4.2:-27132932 MS.gene82336:CDS
AGATGAACACTCCGGCGAAG+TGG 0.626387 4.2:+27133010 None:intergenic
TGGAGAGGAGTTTGACAGGA+CGG 0.627576 4.2:+27133275 None:intergenic
CGGAGTGTTCATCTGATGAA+CGG 0.631386 4.2:-27133001 MS.gene82336:CDS
GCCGTAGTGGAAAACACCTA+CGG 0.636102 4.2:-27133040 MS.gene82336:CDS
GCGAAGTGGTTGGATTCCGT+AGG 0.638817 4.2:+27133024 None:intergenic
GACGGAGCGAATGATTGGAG+AGG 0.656655 4.2:+27133138 None:intergenic
TTCCACTACGGCGATGACTG+AGG 0.662058 4.2:+27133051 None:intergenic
CATATACAGCACGGTGTCCG+CGG 0.662637 4.2:+27132796 None:intergenic
TGATGGCAAAGTTCCTCATG+TGG 0.667427 4.2:-27132693 MS.gene82336:CDS
ATGAACGGAATGAAATACAC+CGG 0.680070 4.2:-27132986 MS.gene82336:CDS
CGTTCATCAGATGAACACTC+CGG 0.693640 4.2:+27133002 None:intergenic
GAAGCCGTGGAAGACGCTGT+GGG 0.695466 4.2:+27133303 None:intergenic
TGATTCGTTTGGATTCAGAG+AGG 0.703589 4.2:+27133608 None:intergenic
GATGCTTTACAACGCCGCCG+CGG 0.710091 4.2:-27132813 MS.gene82336:CDS
ACTGGCGGAGGAGATTGTGG+AGG 0.713390 4.2:-27132750 MS.gene82336:CDS
TTGACGAAGAAGGATGTGGA+TGG 0.715448 4.2:+27133465 None:intergenic
ATACAGCACGGTGTCCGCGG+CGG 0.729974 4.2:+27132799 None:intergenic
CTTACAGTACATATACAGCA+CGG 0.737948 4.2:+27132787 None:intergenic
TGTACAACAGAAACCACATG+AGG 0.740386 4.2:+27132680 None:intergenic
CATCAACACTGTCATCACGG+CGG 0.807246 4.2:+27132832 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
ATGAACGGAATGAAATACAC+CGG - chr4.2:27133387-27133406 MS.gene82336:CDS 35.0%
CCAAAGAAGCAAAGAACAAA+AGG + chr4.2:27133422-27133441 None:intergenic 35.0%
CTTACAGTACATATACAGCA+CGG + chr4.2:27133589-27133608 None:intergenic 35.0%
GAAGGAGAGATATTTGATGA+TGG + chr4.2:27132679-27132698 None:intergenic 35.0%
GAGAGAAAGAACAAGAAGAT+AGG + chr4.2:27133027-27133046 None:intergenic 35.0%
TCTTCTACATCCGTATATCA+TGG - chr4.2:27133292-27133311 MS.gene82336:CDS 35.0%
TTGTTCTTTGCTTCTTTGGA+AGG - chr4.2:27133423-27133442 MS.gene82336:CDS 35.0%
! CCTTTTGTTCTTTGCTTCTT+TGG - chr4.2:27133419-27133438 MS.gene82336:CDS 35.0%
! CTTTGGAAGGAATTATGTTG+TGG - chr4.2:27133436-27133455 MS.gene82336:CDS 35.0%
!! TTGTTTCTGATAGTGGTTCA+TGG - chr4.2:27133481-27133500 MS.gene82336:CDS 35.0%
AAGATAGGCAACAAGAGAAG+TGG + chr4.2:27133012-27133031 None:intergenic 40.0%
AAGCATCAACACTGTCATCA+CGG + chr4.2:27133547-27133566 None:intergenic 40.0%
AGGAGAGAGTCCATGATATA+CGG + chr4.2:27133305-27133324 None:intergenic 40.0%
ATCAAGAGAAGTGGTAAACC+TGG + chr4.2:27132974-27132993 None:intergenic 40.0%
ATGTACTGTAAGGCTGTACA+TGG - chr4.2:27133597-27133616 MS.gene82336:CDS 40.0%
CGTGCTGTATATGTACTGTA+AGG - chr4.2:27133587-27133606 MS.gene82336:CDS 40.0%
GAAGGAATTATGTTGTGGAG+TGG - chr4.2:27133441-27133460 MS.gene82336:CDS 40.0%
GGAACTTTGCCATCATCAAA+AGG + chr4.2:27133675-27133694 None:intergenic 40.0%
TACGGATGTAGAAGATGAGA+AGG + chr4.2:27133287-27133306 None:intergenic 40.0%
TGATTCGTTTGGATTCAGAG+AGG + chr4.2:27132768-27132787 None:intergenic 40.0%
TGTACAACAGAAACCACATG+AGG + chr4.2:27133696-27133715 None:intergenic 40.0%
! GTGAGTTTGCCTTTTGATGA+TGG - chr4.2:27133663-27133682 MS.gene82336:CDS 40.0%
!! GCTAGAGTTGTTTCTGATAG+TGG - chr4.2:27133474-27133493 MS.gene82336:CDS 40.0%
!! TTCTGATAGTGGTTCATGGA+GGG - chr4.2:27133485-27133504 MS.gene82336:CDS 40.0%
!! TTTCTGATAGTGGTTCATGG+AGG - chr4.2:27133484-27133503 MS.gene82336:CDS 40.0%
AACAAGAGAAGTGGTAAGGG+GGG + chr4.2:27133003-27133022 None:intergenic 45.0%
AAGAAAGGAGGAGATGAGAG+AGG + chr4.2:27133130-27133149 None:intergenic 45.0%
AGATTGTGGAGGAGTTTGCA+TGG - chr4.2:27133634-27133653 MS.gene82336:CDS 45.0%
AGGCAACAAGAGAAGTGGTA+AGG + chr4.2:27133007-27133026 None:intergenic 45.0%
AGTTGCACACAGAAGATACC+AGG - chr4.2:27132953-27132972 MS.gene82336:CDS 45.0%
ATAGTGGTTCATGGAGGGAT+TGG - chr4.2:27133490-27133509 MS.gene82336:CDS 45.0%
ATTCAGAGAGGATGGACCAT+AGG + chr4.2:27132756-27132775 None:intergenic 45.0%
CAACAAGAGAAGTGGTAAGG+GGG + chr4.2:27133004-27133023 None:intergenic 45.0%
CGTTCATCAGATGAACACTC+CGG + chr4.2:27133374-27133393 None:intergenic 45.0%
GAACAAAAGGCAAGAAGCTC+CGG + chr4.2:27133409-27133428 None:intergenic 45.0%
GCAACAAGAGAAGTGGTAAG+GGG + chr4.2:27133005-27133024 None:intergenic 45.0%
GGCAACAAGAGAAGTGGTAA+GGG + chr4.2:27133006-27133025 None:intergenic 45.0%
GGGAGGAAAATCAAGAGAAG+TGG + chr4.2:27132983-27133002 None:intergenic 45.0%
TAAGGCTGTACATGGTGAAC+TGG - chr4.2:27133605-27133624 MS.gene82336:CDS 45.0%
TAGTGGTTCATGGAGGGATT+GGG - chr4.2:27133491-27133510 MS.gene82336:CDS 45.0%
TCGTTTGGATTCAGAGAGGA+TGG + chr4.2:27132764-27132783 None:intergenic 45.0%
TGATGGCAAAGTTCCTCATG+TGG - chr4.2:27133680-27133699 MS.gene82336:CDS 45.0%
TGTTGATGTAGTTGTCGCCA+TGG + chr4.2:27132722-27132741 None:intergenic 45.0%
TTGACGAAGAAGGATGTGGA+TGG + chr4.2:27132911-27132930 None:intergenic 45.0%
TTGCTTGACGAAGAAGGATG+TGG + chr4.2:27132915-27132934 None:intergenic 45.0%
! AGATGAGAAGGAAGAGTGCA+AGG + chr4.2:27133275-27133294 None:intergenic 45.0%
! ATGGAGGGATTGGGCTTTTA+TGG - chr4.2:27133500-27133519 MS.gene82336:CDS 45.0%
! CGGAGTGTTCATCTGATGAA+CGG - chr4.2:27133372-27133391 MS.gene82336:CDS 45.0%
! TCCGTAGGTGTTTTCCACTA+CGG + chr4.2:27133337-27133356 None:intergenic 45.0%
!! GTGAGCGTTGATGATTCGTT+TGG + chr4.2:27132779-27132798 None:intergenic 45.0%
ACGAAGAAGGATGTGGATGG+TGG + chr4.2:27132908-27132927 None:intergenic 50.0%
ACGATGAGGGAGAAAGAGAG+AGG + chr4.2:27132841-27132860 None:intergenic 50.0%
AGAGGATGGACCATAGGTTC+AGG + chr4.2:27132750-27132769 None:intergenic 50.0%
AGATGGAGAAGAGGATGAGG+TGG + chr4.2:27133179-27133198 None:intergenic 50.0%
CAACAAGACAGCTGCAACCA+TGG - chr4.2:27132702-27132721 MS.gene82336:CDS 50.0%
CAACAGCAAGCCTGAACCTA+TGG - chr4.2:27132737-27132756 MS.gene82336:CDS 50.0%
CATCAACACTGTCATCACGG+CGG + chr4.2:27133544-27133563 None:intergenic 50.0%
CATGGTTGCAGCTGTCTTGT+TGG + chr4.2:27132704-27132723 None:intergenic 50.0%
CGAAGAAGGATGTGGATGGT+GGG + chr4.2:27132907-27132926 None:intergenic 50.0%
CGATGAGGGAGAAAGAGAGA+GGG + chr4.2:27132840-27132859 None:intergenic 50.0%
CGGAAAGAGCAAGGAAGTGA+CGG + chr4.2:27132804-27132823 None:intergenic 50.0%
GAAGAAGGATGTGGATGGTG+GGG + chr4.2:27132906-27132925 None:intergenic 50.0%
GAGACGTTGCTTGACGAAGA+AGG + chr4.2:27132921-27132940 None:intergenic 50.0%
GATGGAGAAGAGGATGAGGT+GGG + chr4.2:27133178-27133197 None:intergenic 50.0%
GATGGTAGGAGAGACGATGA+GGG + chr4.2:27132854-27132873 None:intergenic 50.0%
GCGAATGATTGGAGAGGAGA+AGG + chr4.2:27133232-27133251 None:intergenic 50.0%
GGAAAGAGCAAGGAAGTGAC+GGG + chr4.2:27132803-27132822 None:intergenic 50.0%
TGGAGAGGAGTTTGACAGGA+CGG + chr4.2:27133101-27133120 None:intergenic 50.0%
TGTGGTGAGGAGAGGAAGAA+AGG + chr4.2:27133145-27133164 None:intergenic 50.0%
TTGGAGAGGAGAAGGAGATC+AGG + chr4.2:27133224-27133243 None:intergenic 50.0%
! AAGAAGGATGTGGATGGTGG+GGG + chr4.2:27132905-27132924 None:intergenic 50.0%
! GCCGTAGTGGAAAACACCTA+CGG - chr4.2:27133333-27133352 MS.gene82336:CDS 50.0%
! TGAGAAGGAAGAGTGCAAGG+AGG + chr4.2:27133272-27133291 None:intergenic 50.0%
!! GGAAGATGACGGAAAGAGCA+AGG + chr4.2:27132813-27132832 None:intergenic 50.0%
AAGAGAAGTGGTAAGGGGGG+AGG + chr4.2:27133000-27133019 None:intergenic 55.0%
ACGGAATCCAACCACTTCGC+CGG - chr4.2:27133352-27133371 MS.gene82336:CDS 55.0%
ACGGTGGAGAGGAGTTTGAC+AGG + chr4.2:27133105-27133124 None:intergenic 55.0%
AGAAGAGGATGAGGTGGGAG+AGG + chr4.2:27133173-27133192 None:intergenic 55.0%
AGATGAACACTCCGGCGAAG+TGG + chr4.2:27133366-27133385 None:intergenic 55.0%
AGCGTCTTCCACGGCTTCTT+TGG - chr4.2:27133075-27133094 MS.gene82336:CDS 55.0%
AGGATGGTTGTGGTGAGGAG+AGG + chr4.2:27133153-27133172 None:intergenic 55.0%
ATCACCCACAGCGTCTTCCA+CGG - chr4.2:27133066-27133085 MS.gene82336:CDS 55.0%
CATATACAGCACGGTGTCCG+CGG + chr4.2:27133580-27133599 None:intergenic 55.0%
GACGGAGCGAATGATTGGAG+AGG + chr4.2:27133238-27133257 None:intergenic 55.0%
GAGAGGAGAAGGAGATCAGG+CGG + chr4.2:27133221-27133240 None:intergenic 55.0%
GAGAGGGAGGAGGAAGATGA+CGG + chr4.2:27132824-27132843 None:intergenic 55.0%
GCGAAGTGGTTGGATTCCGT+AGG + chr4.2:27133352-27133371 None:intergenic 55.0%
GGAGGAGATGAGAGAGGTGA+CGG + chr4.2:27133124-27133143 None:intergenic 55.0%
GGATGGTAGGAGAGACGATG+AGG + chr4.2:27132855-27132874 None:intergenic 55.0%
GGCTGTACATGGTGAACTGG+CGG - chr4.2:27133608-27133627 MS.gene82336:CDS 55.0%
GGGAGATGGAGAAGAGGATG+AGG + chr4.2:27133182-27133201 None:intergenic 55.0%
GGTGAGGAGAGGAAGAAAGG+AGG + chr4.2:27133142-27133161 None:intergenic 55.0%
TGAACTGGCGGAGGAGATTG+TGG - chr4.2:27133620-27133639 MS.gene82336:CDS 55.0%
TGAGGGAGAAAGAGAGAGGG+AGG + chr4.2:27132837-27132856 None:intergenic 55.0%
TGTACATGGTGAACTGGCGG+AGG - chr4.2:27133611-27133630 MS.gene82336:CDS 55.0%
TTCCACTACGGCGATGACTG+AGG + chr4.2:27133325-27133344 None:intergenic 55.0%
! GAAGAGTGCAAGGAGGAGGA+TGG + chr4.2:27133265-27133284 None:intergenic 55.0%
! GAAGGAAGAGTGCAAGGAGG+AGG + chr4.2:27133269-27133288 None:intergenic 55.0%
! GCAGCTGTCTTGTTGGTGGA+AGG + chr4.2:27132697-27132716 None:intergenic 55.0%
! GGTTGCAGCTGTCTTGTTGG+TGG + chr4.2:27132701-27132720 None:intergenic 55.0%
AAGGAGATCAGGCGGAGGAG+GGG + chr4.2:27133213-27133232 None:intergenic 60.0%
ACTGGCGGAGGAGATTGTGG+AGG - chr4.2:27133623-27133642 MS.gene82336:CDS 60.0%
AGAAGCCGTGGAAGACGCTG+TGG + chr4.2:27133074-27133093 None:intergenic 60.0%
AGAAGGAGATCAGGCGGAGG+AGG + chr4.2:27133215-27133234 None:intergenic 60.0%
AGGAGAAGGAGATCAGGCGG+AGG + chr4.2:27133218-27133237 None:intergenic 60.0%
CTCCTCAGTCATCGCCGTAG+TGG - chr4.2:27133320-27133339 MS.gene82336:CDS 60.0%
CTGAGGAGGTGGAGGATGGT+AGG + chr4.2:27132868-27132887 None:intergenic 60.0%
GAACACTCCGGCGAAGTGGT+TGG + chr4.2:27133362-27133381 None:intergenic 60.0%
GAAGCCGTGGAAGACGCTGT+GGG + chr4.2:27133073-27133092 None:intergenic 60.0%
GAAGGAGATCAGGCGGAGGA+GGG + chr4.2:27133214-27133233 None:intergenic 60.0%
GAGGATGAGGTGGGAGAGGA+TGG + chr4.2:27133169-27133188 None:intergenic 60.0%
GAGGTGGGAGAGGATGGTTG+TGG + chr4.2:27133163-27133182 None:intergenic 60.0%
GAGTGCAAGGAGGAGGATGG+CGG + chr4.2:27133262-27133281 None:intergenic 60.0%
GATGCTTTACAACGCCGCCG+CGG - chr4.2:27133560-27133579 MS.gene82336:CDS 60.0%
GCGGAGACGGAGCGAATGAT+TGG + chr4.2:27133243-27133262 None:intergenic 60.0%
GGGAGAAAGAGAGAGGGAGG+AGG + chr4.2:27132834-27132853 None:intergenic 60.0%
GGGAGAGGATGGTTGTGGTG+AGG + chr4.2:27133158-27133177 None:intergenic 60.0%
GTGGAAGACGCTGTGGGTGA+TGG + chr4.2:27133067-27133086 None:intergenic 60.0%
! GAAGACGCTGTGGGTGATGG+TGG + chr4.2:27133064-27133083 None:intergenic 60.0%
! GATGGTGGCGAGGGAGGATA+GGG + chr4.2:27133049-27133068 None:intergenic 60.0%
! GGAGATGAGAGAGGTGACGG+TGG + chr4.2:27133121-27133140 None:intergenic 60.0%
! TGAGAGAGGTGACGGTGGAG+AGG + chr4.2:27133116-27133135 None:intergenic 60.0%
!! AAGGAGGAGGATGGCGGAGA+CGG + chr4.2:27133256-27133275 None:intergenic 60.0%
!! GATGTGGATGGTGGGGGAGT+TGG + chr4.2:27132899-27132918 None:intergenic 60.0%
!! TGATGGTGGCGAGGGAGGAT+AGG + chr4.2:27133050-27133069 None:intergenic 60.0%
!! TGGATGGTGGGGGAGTTGGA+GGG + chr4.2:27132895-27132914 None:intergenic 60.0%
AGGAGATCAGGCGGAGGAGG+GGG + chr4.2:27133212-27133231 None:intergenic 65.0%
ATACAGCACGGTGTCCGCGG+CGG + chr4.2:27133577-27133596 None:intergenic 65.0%
CAGGACGGCCAAAGAAGCCG+TGG + chr4.2:27133086-27133105 None:intergenic 65.0%
CGGGGAGGGAGATGGAGAAG+AGG + chr4.2:27133188-27133207 None:intergenic 65.0%
! GGTGCTGAGGAGGTGGAGGA+TGG + chr4.2:27132872-27132891 None:intergenic 65.0%
!! AGTTGGAGGGGGTGCTGAGG+AGG + chr4.2:27132882-27132901 None:intergenic 65.0%
!! GATGGTGGGGGAGTTGGAGG+GGG + chr4.2:27132893-27132912 None:intergenic 65.0%
!! GCTGTGGGTGATGGTGGCGA+GGG + chr4.2:27133058-27133077 None:intergenic 65.0%
!! GGATGGTGGGGGAGTTGGAG+GGG + chr4.2:27132894-27132913 None:intergenic 65.0%
!! GTGGATGGTGGGGGAGTTGG+AGG + chr4.2:27132896-27132915 None:intergenic 65.0%
ATCAGGCGGAGGAGGGGGAC+GGG + chr4.2:27133207-27133226 None:intergenic 70.0%
GATCAGGCGGAGGAGGGGGA+CGG + chr4.2:27133208-27133227 None:intergenic 70.0%
!! AGGGGGTGCTGAGGAGGTGG+AGG + chr4.2:27132876-27132895 None:intergenic 70.0%
!! CGCTGTGGGTGATGGTGGCG+AGG + chr4.2:27133059-27133078 None:intergenic 70.0%
!! GGGAGTTGGAGGGGGTGCTG+AGG + chr4.2:27132885-27132904 None:intergenic 70.0%
!! GTGGGTGATGGTGGCGAGGG+AGG + chr4.2:27133055-27133074 None:intergenic 70.0%
!! TGGAGGGGGTGCTGAGGAGG+TGG + chr4.2:27132879-27132898 None:intergenic 70.0%
GAGGGGGACGGGGAGGGAGA+TGG + chr4.2:27133196-27133215 None:intergenic 75.0%
TCAGGCGGAGGAGGGGGACG+GGG + chr4.2:27133206-27133225 None:intergenic 75.0%
! GCGGAGGAGGGGGACGGGGA+GGG + chr4.2:27133202-27133221 None:intergenic 80.0%
! GGCGGAGGAGGGGGACGGGG+AGG + chr4.2:27133203-27133222 None:intergenic 85.0%


Chromosome Type Strat End Strand Name
chr4.2 gene 27132673 27133722 27132673 ID=MS.gene82336
chr4.2 mRNA 27132673 27133722 27132673 ID=MS.gene82336.t1;Parent=MS.gene82336
chr4.2 exon 27132673 27133722 27132673 ID=MS.gene82336.t1.exon1;Parent=MS.gene82336.t1
chr4.2 CDS 27132673 27133722 27132673 ID=cds.MS.gene82336.t1;Parent=MS.gene82336.t1
Gene Sequence

>MS.gene82336

ATGCCATCATCAAATATCTCTCCTTCCACCAACAAGACAGCTGCAACCATGGCGACAACTACATCAACAGCAAGCCTGAACCTATGGTCCATCCTCTCTGAATCCAAACGAATCATCAACGCTCACTCCCGTCACTTCCTTGCTCTTTCCGTCATCTTCCTCCTCCCTCTCTCTTTCTCCCTCATCGTCTCTCCTACCATCCTCCACCTCCTCAGCACCCCCTCCAACTCCCCCACCATCCACATCCTTCTTCGTCAAGCAACGTCTCTGCAGTCTCTGCAGTTGCACACAGAAGATACCAGGTTTACCACTTCTCTTGATTTTCCTCCCCCCTTACCACTTCTCTTGTTGCCTATCTTCTTGTTCTTTCTCTCCCTATCCTCCCTCGCCACCATCACCCACAGCGTCTTCCACGGCTTCTTTGGCCGTCCTGTCAAACTCCTCTCCACCGTCACCTCTCTCATCTCCTCCTTTCTTCCTCTCCTCACCACAACCATCCTCTCCCACCTCATCCTCTTCTCCATCTCCCTCCCCGTCCCCCTCCTCCGCCTGATCTCCTTCTCCTCTCCAATCATTCGCTCCGTCTCCGCCATCCTCCTCCTTGCACTCTTCCTTCTCATCTTCTACATCCGTATATCATGGACTCTCTCCTCAGTCATCGCCGTAGTGGAAAACACCTACGGAATCCAACCACTTCGCCGGAGTGTTCATCTGATGAACGGAATGAAATACACCGGAGCTTCTTGCCTTTTGTTCTTTGCTTCTTTGGAAGGAATTATGTTGTGGAGTGGTTTTCTTCTAGCTAGAGTTGTTTCTGATAGTGGTTCATGGAGGGATTGGGCTTTTATGGTTCAGATTGTGTTGACTTCCGCCGTGATGACAGTGTTGATGCTTTACAACGCCGCCGCGGACACCGTGCTGTATATGTACTGTAAGGCTGTACATGGTGAACTGGCGGAGGAGATTGTGGAGGAGTTTGCATGGCAGTATGTGAGTTTGCCTTTTGATGATGGCAAAGTTCCTCATGTGGTTTCTGTTGTACATGCTTGA
Protein sequence

>MS.gene82336.t1

MPSSNISPSTNKTAATMATTTSTASLNLWSILSESKRIINAHSRHFLALSVIFLLPLSFSLIVSPTILHLLSTPSNSPTIHILLRQATSLQSLQLHTEDTRFTTSLDFPPPLPLLLLPIFLFFLSLSSLATITHSVFHGFFGRPVKLLSTVTSLISSFLPLLTTTILSHLILFSISLPVPLLRLISFSSPIIRSVSAILLLALFLLIFYIRISWTLSSVIAVVENTYGIQPLRRSVHLMNGMKYTGASCLLFFASLEGIMLWSGFLLARVVSDSGSWRDWAFMVQIVLTSAVMTVLMLYNAAADTVLYMYCKAVHGELAEEIVEEFAWQYVSLPFDDGKVPHVVSVVHA