AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene82359

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene82359.t1 MTR_4g082910 84.688 320 27 3 7 312 45 356 5.00e-166 466
MS.gene82359.t1 MTR_5g043690 45.397 315 129 9 1 312 38 312 1.02e-55 183
MS.gene82359.t1 MTR_5g043690 45.367 313 128 9 3 312 41 313 4.26e-55 181
MS.gene82359.t1 MTR_3g023650 39.815 108 64 1 205 312 126 232 1.38e-17 80.9
MS.gene82359.t1 MTR_5g043685 38.760 129 61 4 158 286 104 214 6.74e-11 62.4
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene82359.t1 AT1G13920 50.943 106 52 0 207 312 240 345 8.03e-29 113
MS.gene82359.t1 AT1G13920 49.541 109 55 0 204 312 270 378 8.72e-29 114
MS.gene82359.t1 AT5G61280 28.571 189 125 3 126 312 57 237 1.21e-13 69.7
MS.gene82359.t1 AT1G69325 42.342 111 63 1 199 309 10 119 1.76e-13 66.6
MS.gene82359.t1 AT5G61280 28.571 189 125 3 126 312 80 260 2.16e-13 69.3

Find 60 sgRNAs with CRISPR-Local

Find 212 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
ATATTTACCTGATAATCTTT+TGG 0.246925 4.2:-26771220 None:intergenic
ATAGGCTTTGCTTCTGGTCT+TGG 0.265119 4.2:-26771160 None:intergenic
GGTTGTGGTGGCGGCGGAGG+TGG 0.327009 4.2:-26771825 None:intergenic
TTTATCATAGGCTTTGCTTC+TGG 0.328813 4.2:-26771166 None:intergenic
TGTGGTGGCGGCGGAGGTGG+TGG 0.339507 4.2:-26771822 None:intergenic
GTTGCACCTGGTCTAGTTGA+TGG 0.341217 4.2:-26771858 None:intergenic
CGGATATCCTAACACCAAAA+AGG 0.345474 4.2:+26771697 MS.gene82359:CDS
GCTGCATCCAAAAGATTATC+AGG 0.351559 4.2:+26771213 MS.gene82359:CDS
GGCGGAGGTGGTGGCGGCTG+CGG 0.379635 4.2:-26771813 None:intergenic
TCTCTAATAGAAGATGTTGT+TGG 0.380294 4.2:-26771573 None:intergenic
TTTGGATGCAGCACCTATAT+GGG 0.393760 4.2:-26771202 None:intergenic
GGAGGTTGTGGTGGCGGCGG+AGG 0.394059 4.2:-26771828 None:intergenic
AATTTCTACAGAGAAGAAAA+AGG 0.405869 4.2:+26770969 MS.gene82359:intron
GCAAACATTATTAGAACAAC+AGG 0.416708 4.2:+26772567 MS.gene82359:CDS
CAGAAGCAAAAGCAGATACT+TGG 0.418811 4.2:+26771880 MS.gene82359:CDS
GTTCTTTCTTTCTTAGAAGC+AGG 0.419523 4.2:-26772610 None:intergenic
CTGACAGGAGGTTGTGGTGG+CGG 0.433854 4.2:-26771834 None:intergenic
TGTTAGAGACAATAGATTCA+TGG 0.438542 4.2:+26772056 MS.gene82359:CDS
AGAACAACAGGAAAGCTTCC+AGG 0.444025 4.2:+26772579 MS.gene82359:CDS
AAATCTTTACCACCAAAACT+TGG 0.448320 4.2:-26771723 None:intergenic
GCGGCCATGAGGACGTGGTT+GGG 0.450451 4.2:-26771794 None:intergenic
AAAGTGAAAATACACGGAAA+AGG 0.455987 4.2:+26772427 MS.gene82359:CDS
ACAGGAGGTTGTGGTGGCGG+CGG 0.464860 4.2:-26771831 None:intergenic
GGTGGCGGCGGAGGTGGTGG+CGG 0.466003 4.2:-26771819 None:intergenic
TTCATTCTTTCGTCTCTCAT+CGG 0.485034 4.2:-26772523 None:intergenic
AAGTGAAAATACACGGAAAA+GGG 0.489919 4.2:+26772428 MS.gene82359:CDS
TAGAAGCAGGAACAAGCCCC+TGG 0.500961 4.2:-26772597 None:intergenic
TGCGGCCATGAGGACGTGGT+TGG 0.506565 4.2:-26771795 None:intergenic
GTTAGAGACAATAGATTCAT+GGG 0.510246 4.2:+26772057 MS.gene82359:CDS
GATGGTCTGACAGGAGGTTG+TGG 0.513783 4.2:-26771840 None:intergenic
GCTTCTGGTCTTGGCCAATG+AGG 0.527010 4.2:-26771151 None:intergenic
GGTCTAGTTGATGGTCTGAC+AGG 0.535460 4.2:-26771849 None:intergenic
AAAAGCAGATACTTGGGAAA+GGG 0.535761 4.2:+26771887 MS.gene82359:CDS
GAACAACAGGAAAGCTTCCA+GGG 0.544421 4.2:+26772580 MS.gene82359:CDS
GGAGTTGCCTGAGACCTCAT+TGG 0.544976 4.2:+26771137 MS.gene82359:CDS
CGGCTGCGGCCATGAGGACG+TGG 0.549078 4.2:-26771799 None:intergenic
GTCTTGGCCAATGAGGTCTC+AGG 0.549623 4.2:-26771144 None:intergenic
CACGGAAAAGGGAGAAAGCA+TGG 0.551213 4.2:+26772439 MS.gene82359:CDS
TTGTCCCAACCACGTCCTCA+TGG 0.559642 4.2:+26771790 MS.gene82359:CDS
TTCCACATGAGCAGAAAATG+TGG 0.560420 4.2:+26771115 MS.gene82359:CDS
AGAAGCAAAAGCAGATACTT+GGG 0.563207 4.2:+26771881 MS.gene82359:CDS
TGATGATGCTGGTGCGAATG+AGG 0.565873 4.2:-26771619 None:intergenic
AACGCTGAAAGCAAAAGATA+AGG 0.570182 4.2:+26772545 MS.gene82359:CDS
TGCTGCAGCAGCTACCGCTG+TGG 0.578435 4.2:-26771064 None:intergenic
CAAAAGCAGATACTTGGGAA+AGG 0.582056 4.2:+26771886 MS.gene82359:CDS
ACTACAGCTGTAACTATGAA+AGG 0.582124 4.2:+26768652 None:intergenic
CAATAGATTCATGGGAAAAG+CGG 0.584674 4.2:+26772065 MS.gene82359:CDS
CACAGATGAACAAGGAAACA+AGG 0.591861 4.2:+26771548 MS.gene82359:CDS
TGGTGGCGGCTGCGGCCATG+AGG 0.604575 4.2:-26771805 None:intergenic
TATATAGACGAGATTGCAGA+AGG 0.610293 4.2:+26772486 MS.gene82359:CDS
TTTAAAATCACAGATGAACA+AGG 0.610365 4.2:+26771540 MS.gene82359:CDS
GGTCTGACAGGAGGTTGTGG+TGG 0.627760 4.2:-26771837 None:intergenic
CTAGTTGATGGTCTGACAGG+AGG 0.633454 4.2:-26771846 None:intergenic
GTCAGACCATCAACTAGACC+AGG 0.640427 4.2:+26771852 MS.gene82359:CDS
TGCAGCAAAGTGAAAATACA+CGG 0.640916 4.2:+26772421 MS.gene82359:intron
TCCACATGAGCAGAAAATGT+GGG 0.644028 4.2:+26771116 MS.gene82359:CDS
ACGCAAGCTAAACAAGCATG+AGG 0.679702 4.2:+26772105 MS.gene82359:CDS
TGATGCTGGTGCGAATGAGG+TGG 0.686427 4.2:-26771616 None:intergenic
AACAACAGGAAAGCTTCCAG+GGG 0.710912 4.2:+26772581 MS.gene82359:CDS
GGAAATGGAGCATGCCACAG+CGG 0.741947 4.2:+26771050 MS.gene82359:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AAATATAAAAAAATGAATGA+TGG + chr4.2:26770863-26770882 MS.gene82359:intron 10.0%
!! CAAAAAAAAAAAAAAATAGA+CGG + chr4.2:26769714-26769733 MS.gene82359:intron 10.0%
!! TTTACTATTTATAAATAGTT+GGG + chr4.2:26769331-26769350 MS.gene82359:intron 10.0%
!!! ATTTACATTTTAAATTCTAA+TGG + chr4.2:26769749-26769768 MS.gene82359:intron 10.0%
!!! TAATTTATAGTTTTTGTTTT+TGG + chr4.2:26770903-26770922 MS.gene82359:intron 10.0%
!! ATAAGTAATAACTTCAATAT+CGG + chr4.2:26769379-26769398 MS.gene82359:intron 15.0%
!! ATATTTAATGTTTACAAACA+TGG + chr4.2:26769032-26769051 MS.gene82359:intron 15.0%
!! GAAAACTTGATTATAAAATA+AGG + chr4.2:26770189-26770208 MS.gene82359:intron 15.0%
!! GTTTACTATTTATAAATAGT+TGG + chr4.2:26769330-26769349 MS.gene82359:intron 15.0%
!! TTAATTTATGTGTATAAAGA+AGG - chr4.2:26769996-26770015 None:intergenic 15.0%
!!! AAATGAAGATTTTTTATCAT+AGG - chr4.2:26771181-26771200 None:intergenic 15.0%
!!! AATACTTTTAATCTCTAAAA+GGG - chr4.2:26770025-26770044 None:intergenic 15.0%
!!! ATGTTTTGATTATTTGTATA+AGG + chr4.2:26768973-26768992 MS.gene82359:intron 15.0%
!!! TAATACTTTTAATCTCTAAA+AGG - chr4.2:26770026-26770045 None:intergenic 15.0%
!!! TTTAGTATTTCAATTTTCAA+TGG - chr4.2:26770846-26770865 None:intergenic 15.0%
!!! TTTTATATATCAAGAAGAAT+TGG - chr4.2:26770595-26770614 None:intergenic 15.0%
!! AATATTAGTTGATTGTTATG+TGG + chr4.2:26769811-26769830 MS.gene82359:intron 20.0%
!! AGTTTAATCAGCTTATAATT+AGG - chr4.2:26772254-26772273 None:intergenic 20.0%
!! ATACTATGTCATATATTTCA+TGG - chr4.2:26768930-26768949 None:intergenic 20.0%
!! ATATCAAATTAAATCAGCTA+AGG - chr4.2:26770150-26770169 None:intergenic 20.0%
!! CTACAATTACAATTGTTTAT+CGG + chr4.2:26769165-26769184 MS.gene82359:intron 20.0%
!! GAAAATATAATCAATTTGTC+AGG + chr4.2:26772023-26772042 MS.gene82359:intron 20.0%
!! GAATTATATTCATGTATAGA+TGG + chr4.2:26768882-26768901 MS.gene82359:intron 20.0%
!! GACAATCTTAAATTTCTATA+AGG - chr4.2:26770517-26770536 None:intergenic 20.0%
!! TACAAATGAAAAATAGTTGT+TGG + chr4.2:26769094-26769113 MS.gene82359:intron 20.0%
!! TACTATGTCATATATTTCAT+GGG - chr4.2:26768929-26768948 None:intergenic 20.0%
!! TAGACAAAACTAATTCTAAT+CGG + chr4.2:26769578-26769597 MS.gene82359:intron 20.0%
!! TTGAAAAAATCTGATGAAAA+TGG - chr4.2:26769948-26769967 None:intergenic 20.0%
!! TTTGTTAGAGAGAAATTAAT+TGG + chr4.2:26770644-26770663 MS.gene82359:intron 20.0%
!!! ATATTTACCTGATAATCTTT+TGG - chr4.2:26771223-26771242 None:intergenic 20.0%
!!! ATTTCTAAAATTTTCCCAAT+TGG + chr4.2:26769287-26769306 MS.gene82359:intron 20.0%
!!! TGATTATGATTTTATGGAAA+TGG + chr4.2:26771035-26771054 MS.gene82359:CDS 20.0%
! AAAATTCTTCCTGTTTAGAA+TGG + chr4.2:26770448-26770467 MS.gene82359:intron 25.0%
! AATTTCTACAGAGAAGAAAA+AGG + chr4.2:26770969-26770988 MS.gene82359:intron 25.0%
! ACTTATGAGTACATGTTATA+GGG - chr4.2:26769365-26769384 None:intergenic 25.0%
! AGACAAAACTAATTCTAATC+GGG + chr4.2:26769579-26769598 MS.gene82359:intron 25.0%
! CTATTTCATGTTTGGATAAT+TGG - chr4.2:26769673-26769692 None:intergenic 25.0%
! CTTCTATTAGAGAAGAAAAA+AGG + chr4.2:26771583-26771602 MS.gene82359:CDS 25.0%
! GAGAAATGATGATAAAAAAC+CGG + chr4.2:26771677-26771696 MS.gene82359:CDS 25.0%
! GTAAATGAACATATCCAATT+GGG - chr4.2:26769304-26769323 None:intergenic 25.0%
! TAAATCAGCTAAGGAAAAAA+GGG - chr4.2:26770141-26770160 None:intergenic 25.0%
! TACTTATGAGTACATGTTAT+AGG - chr4.2:26769366-26769385 None:intergenic 25.0%
! TATGACTATACTCTACAAAA+AGG + chr4.2:26770362-26770381 MS.gene82359:intron 25.0%
! TCAAGCATGTATTTATATGA+AGG + chr4.2:26769898-26769917 MS.gene82359:intron 25.0%
! TGTAAATGAACATATCCAAT+TGG - chr4.2:26769305-26769324 None:intergenic 25.0%
! TGTATTCTTAGGGATAAATT+CGG + chr4.2:26771371-26771390 MS.gene82359:intron 25.0%
! TGTGGAATAAATTAAAGAAC+TGG - chr4.2:26770061-26770080 None:intergenic 25.0%
! TTAAATCAGCTAAGGAAAAA+AGG - chr4.2:26770142-26770161 None:intergenic 25.0%
! TTAACGTTCTTGTATTCTTA+GGG + chr4.2:26771361-26771380 MS.gene82359:intron 25.0%
! TTCAATATCGGATGAAATTT+AGG + chr4.2:26769391-26769410 MS.gene82359:intron 25.0%
! TTTAACGTTCTTGTATTCTT+AGG + chr4.2:26771360-26771379 MS.gene82359:intron 25.0%
!! AAAGATGAACTTTTCTGTAT+TGG - chr4.2:26770722-26770741 None:intergenic 25.0%
!! AAATCTTCATTTTCCCATAT+AGG + chr4.2:26771189-26771208 MS.gene82359:CDS 25.0%
!! CTTTGAACTTTTATGTACAT+TGG - chr4.2:26770086-26770105 None:intergenic 25.0%
!! GAGTCATGATTATGATTTTA+TGG + chr4.2:26771029-26771048 MS.gene82359:CDS 25.0%
!! TCAATTTTCAATGGCTTTTT+TGG - chr4.2:26770837-26770856 None:intergenic 25.0%
!! TTTAAAATCACAGATGAACA+AGG + chr4.2:26771540-26771559 MS.gene82359:CDS 25.0%
!! TTTTTGTAGAGTATAGTCAT+AGG - chr4.2:26770363-26770382 None:intergenic 25.0%
!!! CAATTTTCAATGGCTTTTTT+GGG - chr4.2:26770836-26770855 None:intergenic 25.0%
!!! TTTGATTTTTCACCAAAAGT+AGG - chr4.2:26771657-26771676 None:intergenic 25.0%
!!! TTTTCTGTATTGGTATTTTC+TGG - chr4.2:26770712-26770731 None:intergenic 25.0%
AAAGTGAAAATACACGGAAA+AGG + chr4.2:26772427-26772446 MS.gene82359:CDS 30.0%
AAATAAGATCCAATGCTTGA+TGG + chr4.2:26770224-26770243 MS.gene82359:intron 30.0%
AAATCTTTACCACCAAAACT+TGG - chr4.2:26771726-26771745 None:intergenic 30.0%
AAGAACGTTAAAAACTCTTG+AGG - chr4.2:26771352-26771371 None:intergenic 30.0%
AAGTGAAAATACACGGAAAA+GGG + chr4.2:26772428-26772447 MS.gene82359:CDS 30.0%
AGAACGTTAAAAACTCTTGA+GGG - chr4.2:26771351-26771370 None:intergenic 30.0%
AGCTCAAAAAGATCAAAGAA+AGG + chr4.2:26771913-26771932 MS.gene82359:CDS 30.0%
ATTAAAGTGGTTATCCAAAC+AGG - chr4.2:26772151-26772170 None:intergenic 30.0%
CAAAAAGGTTCTCATTTCTT+TGG + chr4.2:26770377-26770396 MS.gene82359:intron 30.0%
CAGAAAAGTTCATCTTTCAA+AGG + chr4.2:26770725-26770744 MS.gene82359:intron 30.0%
CTAACTAAAATGTACGTAAG+AGG - chr4.2:26769129-26769148 None:intergenic 30.0%
CTTTAATTATGATGAGTGGT+AGG - chr4.2:26770425-26770444 None:intergenic 30.0%
GATGAGATCTATTTCATGTT+TGG - chr4.2:26769681-26769700 None:intergenic 30.0%
GCAAACATTATTAGAACAAC+AGG + chr4.2:26772567-26772586 MS.gene82359:CDS 30.0%
GGATATGTTCATTTACATGA+TGG + chr4.2:26769308-26769327 MS.gene82359:intron 30.0%
GTTAATTCTCTGATAAAGTG+TGG + chr4.2:26772382-26772401 MS.gene82359:intron 30.0%
GTTAGAGACAATAGATTCAT+GGG + chr4.2:26772057-26772076 MS.gene82359:CDS 30.0%
TATTCATGTATAGATGGAGT+TGG + chr4.2:26768888-26768907 MS.gene82359:intron 30.0%
TCCACATTAATGAAACTCAT+GGG - chr4.2:26768780-26768799 None:intergenic 30.0%
TCGAGTGTCAAAATCATAAT+TGG + chr4.2:26769527-26769546 MS.gene82359:intron 30.0%
TCTCTAATAGAAGATGTTGT+TGG - chr4.2:26771576-26771595 None:intergenic 30.0%
TCTGAAAACAAACATGGAAA+TGG + chr4.2:26768704-26768723 MS.gene82359:intron 30.0%
TGTGCTTTAATTATGATGAG+TGG - chr4.2:26770429-26770448 None:intergenic 30.0%
TGTTAGAGACAATAGATTCA+TGG + chr4.2:26772056-26772075 MS.gene82359:CDS 30.0%
TTAAAGTGGTTATCCAAACA+GGG - chr4.2:26772150-26772169 None:intergenic 30.0%
TTCATAAGATCAAGAGTCTT+TGG - chr4.2:26769846-26769865 None:intergenic 30.0%
! AGAAGAAAAAGGCACATAAT+TGG + chr4.2:26770980-26770999 MS.gene82359:CDS 30.0%
! AGTTTTTGTATGTTTGACCT+TGG - chr4.2:26769610-26769629 None:intergenic 30.0%
! ATAGTCATAGGTTGTTATTG+AGG - chr4.2:26770351-26770370 None:intergenic 30.0%
! ATGAAATTTAGGTTCTACGA+CGG + chr4.2:26769402-26769421 MS.gene82359:intron 30.0%
! ATGACAAAAACTCCTACTTT+TGG + chr4.2:26771642-26771661 MS.gene82359:CDS 30.0%
! GAGGTTAGATTTTCATATTC+AGG + chr4.2:26772124-26772143 MS.gene82359:intron 30.0%
! TAGTCATAGGTTGTTATTGA+GGG - chr4.2:26770350-26770369 None:intergenic 30.0%
!! CATTTTAACCATTTAGTTGG+GGG + chr4.2:26769055-26769074 MS.gene82359:intron 30.0%
!! GAAGAGAATACAACTGATTT+TGG + chr4.2:26770674-26770693 MS.gene82359:intron 30.0%
!! GACTTTGTTATCAGCATTTT+AGG + chr4.2:26770771-26770790 MS.gene82359:intron 30.0%
!! GCATTTTAACCATTTAGTTG+GGG + chr4.2:26769054-26769073 MS.gene82359:intron 30.0%
!! GGCATTTTAACCATTTAGTT+GGG + chr4.2:26769053-26769072 MS.gene82359:intron 30.0%
!! TGGCATTTTAACCATTTAGT+TGG + chr4.2:26769052-26769071 MS.gene82359:intron 30.0%
!! TTTTCAAGAACAGAGCAAAA+AGG + chr4.2:26769961-26769980 MS.gene82359:intron 30.0%
!!! GAGAATTAACAAGAGCATTT+TGG - chr4.2:26772372-26772391 None:intergenic 30.0%
!!! GATTTTTTACCATCAAGCAT+TGG - chr4.2:26770236-26770255 None:intergenic 30.0%
!!! TTGGGACAATTTTTGGTTTT+GGG - chr4.2:26771779-26771798 None:intergenic 30.0%
AAAAGCAGATACTTGGGAAA+GGG + chr4.2:26771887-26771906 MS.gene82359:CDS 35.0%
AAATCAAACCCCCAACTAAA+TGG - chr4.2:26769066-26769085 None:intergenic 35.0%
AACGCTGAAAGCAAAAGATA+AGG + chr4.2:26772545-26772564 MS.gene82359:CDS 35.0%
AAGCATAATTGAGGATGAAG+AGG + chr4.2:26768752-26768771 MS.gene82359:intron 35.0%
ACTCAATATGCATCACTTGT+AGG + chr4.2:26771513-26771532 MS.gene82359:intron 35.0%
AGAAGCAAAAGCAGATACTT+GGG + chr4.2:26771881-26771900 MS.gene82359:CDS 35.0%
ATAGATACGTCCTAGACTTT+CGG - chr4.2:26768807-26768826 None:intergenic 35.0%
CAATAGATTCATGGGAAAAG+CGG + chr4.2:26772065-26772084 MS.gene82359:CDS 35.0%
CCACATTAATGAAACTCATG+GGG - chr4.2:26768779-26768798 None:intergenic 35.0%
CGAGAATCTGAAAACAAACA+TGG + chr4.2:26768698-26768717 MS.gene82359:intron 35.0%
CTCCACATTAATGAAACTCA+TGG - chr4.2:26768781-26768800 None:intergenic 35.0%
GACTCTTGATCTTATGAACT+AGG + chr4.2:26769848-26769867 MS.gene82359:intron 35.0%
TATACTTTGCATGTGTTGTC+TGG - chr4.2:26770312-26770331 None:intergenic 35.0%
TATATAGACGAGATTGCAGA+AGG + chr4.2:26772486-26772505 MS.gene82359:CDS 35.0%
TGCAGCAAAGTGAAAATACA+CGG + chr4.2:26772421-26772440 MS.gene82359:intron 35.0%
TGTTGCTCACAAGAAGAATA+TGG + chr4.2:26768834-26768853 MS.gene82359:intron 35.0%
TTCATTCTTTCGTCTCTCAT+CGG - chr4.2:26772526-26772545 None:intergenic 35.0%
TTTATCATAGGCTTTGCTTC+TGG - chr4.2:26771169-26771188 None:intergenic 35.0%
! AGTCATAGGTTGTTATTGAG+GGG - chr4.2:26770349-26770368 None:intergenic 35.0%
! CTTTTATGTACATTGGCATG+TGG - chr4.2:26770079-26770098 None:intergenic 35.0%
! GATGGTTGAAAGCATAATTG+AGG + chr4.2:26768743-26768762 MS.gene82359:intron 35.0%
! GGTTTCAGAGACAATTTTCA+AGG + chr4.2:26771001-26771020 MS.gene82359:CDS 35.0%
! GTCTAGGACGTATCTATTTA+TGG + chr4.2:26768810-26768829 MS.gene82359:intron 35.0%
! TCCTGTTTAGAATGGAGAAT+TGG + chr4.2:26770456-26770475 MS.gene82359:intron 35.0%
! TTTTGTATGTTTGACCTTGG+TGG - chr4.2:26769607-26769626 None:intergenic 35.0%
!! AACTGATTTTGGTGGTAGTA+GGG + chr4.2:26770685-26770704 MS.gene82359:intron 35.0%
!! GAGAATACAACTGATTTTGG+TGG + chr4.2:26770677-26770696 MS.gene82359:intron 35.0%
!! TTTTGGCTGAAAACTGAATG+AGG + chr4.2:26770920-26770939 MS.gene82359:intron 35.0%
!!! CTTTTTGGTGTTAGGATATC+CGG - chr4.2:26771699-26771718 None:intergenic 35.0%
!!! GTTGGGACAATTTTTGGTTT+TGG - chr4.2:26771780-26771799 None:intergenic 35.0%
!!! GTTTTTGTCATTGATGATGC+TGG - chr4.2:26771633-26771652 None:intergenic 35.0%
!!! TGGGACAATTTTTGGTTTTG+GGG - chr4.2:26771778-26771797 None:intergenic 35.0%
AATACTTGTCTTCACCCACA+AGG + chr4.2:26770271-26770290 MS.gene82359:intron 40.0%
AGAATTGGCTGAAGCAGATA+AGG + chr4.2:26770471-26770490 MS.gene82359:intron 40.0%
CAAAAGCAGATACTTGGGAA+AGG + chr4.2:26771886-26771905 MS.gene82359:CDS 40.0%
CACAGATGAACAAGGAAACA+AGG + chr4.2:26771548-26771567 MS.gene82359:CDS 40.0%
CAGAAGCAAAAGCAGATACT+TGG + chr4.2:26771880-26771899 MS.gene82359:CDS 40.0%
CCCCATGAGTTTCATTAATG+TGG + chr4.2:26768776-26768795 MS.gene82359:intron 40.0%
CGGATATCCTAACACCAAAA+AGG + chr4.2:26771697-26771716 MS.gene82359:CDS 40.0%
GATTCAAAATCCACACGAGT+TGG - chr4.2:26769880-26769899 None:intergenic 40.0%
GCCAATTCTCCATTCTAAAC+AGG - chr4.2:26770460-26770479 None:intergenic 40.0%
GCTGATAACAAAGTCTGCAA+GGG - chr4.2:26770766-26770785 None:intergenic 40.0%
GCTGCATCCAAAAGATTATC+AGG + chr4.2:26771213-26771232 MS.gene82359:CDS 40.0%
GGTTAGAGAGAGAAAGAAGA+TGG + chr4.2:26768725-26768744 MS.gene82359:intron 40.0%
GTGTAAGCGCTCAATTAAAG+TGG - chr4.2:26772164-26772183 None:intergenic 40.0%
TCCACATGAGCAGAAAATGT+GGG + chr4.2:26771116-26771135 MS.gene82359:CDS 40.0%
TGCTGATAACAAAGTCTGCA+AGG - chr4.2:26770767-26770786 None:intergenic 40.0%
TGTTTATCGGAGAGTCACAT+TGG + chr4.2:26769178-26769197 MS.gene82359:intron 40.0%
TTCCACATGAGCAGAAAATG+TGG + chr4.2:26771115-26771134 MS.gene82359:CDS 40.0%
TTTCATATTCAGGCCCTGTT+TGG + chr4.2:26772134-26772153 MS.gene82359:intron 40.0%
TTTGCATGTGTTGTCTGGTT+TGG - chr4.2:26770307-26770326 None:intergenic 40.0%
TTTGGATGCAGCACCTATAT+GGG - chr4.2:26771205-26771224 None:intergenic 40.0%
! CCAAAAAGGACTCCAAGTTT+TGG + chr4.2:26771711-26771730 MS.gene82359:CDS 40.0%
! CCAAAACTTGGAGTCCTTTT+TGG - chr4.2:26771714-26771733 None:intergenic 40.0%
! TTGGATTTCAAGTGCCTTGT+GGG - chr4.2:26770288-26770307 None:intergenic 40.0%
! TTGTTATTGAGGGGAAAGCA+AGG - chr4.2:26770340-26770359 None:intergenic 40.0%
! TTTGGATTTCAAGTGCCTTG+TGG - chr4.2:26770289-26770308 None:intergenic 40.0%
! TTTTGGATGCAGCACCTATA+TGG - chr4.2:26771206-26771225 None:intergenic 40.0%
!! AAAAGGACTCCAAGTTTTGG+TGG + chr4.2:26771714-26771733 MS.gene82359:CDS 40.0%
!! CAACTGATTTTGGTGGTAGT+AGG + chr4.2:26770684-26770703 MS.gene82359:intron 40.0%
!!! ATTTTTGGTTTTGGGGTCTC+AGG - chr4.2:26771771-26771790 None:intergenic 40.0%
!!! TTGGAGTCCTTTTTGGTGTT+AGG - chr4.2:26771707-26771726 None:intergenic 40.0%
AACAACAGGAAAGCTTCCAG+GGG + chr4.2:26772581-26772600 MS.gene82359:CDS 45.0%
AATTCGGACTCACTACCTCT+TGG + chr4.2:26771387-26771406 MS.gene82359:intron 45.0%
ACGCAAGCTAAACAAGCATG+AGG + chr4.2:26772105-26772124 MS.gene82359:CDS 45.0%
AGAACAACAGGAAAGCTTCC+AGG + chr4.2:26772579-26772598 MS.gene82359:CDS 45.0%
ATAGGCTTTGCTTCTGGTCT+TGG - chr4.2:26771163-26771182 None:intergenic 45.0%
ATTCTAATCGGGAACCACCA+AGG + chr4.2:26769590-26769609 MS.gene82359:intron 45.0%
CAAGTCTCTTTCAGTCCAAG+AGG - chr4.2:26771405-26771424 None:intergenic 45.0%
CTGAGTAGAACCACAATGTG+CGG + chr4.2:26771316-26771335 MS.gene82359:intron 45.0%
GAACAACAGGAAAGCTTCCA+GGG + chr4.2:26772580-26772599 MS.gene82359:CDS 45.0%
! GACGTGGTTGGGACAATTTT+TGG - chr4.2:26771786-26771805 None:intergenic 45.0%
! TCCCACATTTTCTGCTCATG+TGG - chr4.2:26771120-26771139 None:intergenic 45.0%
CACGGAAAAGGGAGAAAGCA+TGG + chr4.2:26772439-26772458 MS.gene82359:CDS 50.0%
GATACGTCCTAGACTTTCGG+TGG - chr4.2:26768804-26768823 None:intergenic 50.0%
GTCAGACCATCAACTAGACC+AGG + chr4.2:26771852-26771871 MS.gene82359:CDS 50.0%
GTTGCACCTGGTCTAGTTGA+TGG - chr4.2:26771861-26771880 None:intergenic 50.0%
TAGAACCACAATGTGCGGCA+AGG + chr4.2:26771321-26771340 MS.gene82359:intron 50.0%
TAGGAATCAGCCAACTCGTG+TGG + chr4.2:26769867-26769886 MS.gene82359:intron 50.0%
! GCGAATGAGGTGGTCTTTTC+AGG - chr4.2:26771609-26771628 None:intergenic 50.0%
! GCTTTTGCTTCTGTTGCACC+TGG - chr4.2:26771873-26771892 None:intergenic 50.0%
! TGATGATGCTGGTGCGAATG+AGG - chr4.2:26771622-26771641 None:intergenic 50.0%
! TGTGGTTCTACTCAGCTCGA+TGG - chr4.2:26771311-26771330 None:intergenic 50.0%
!! CTAGTTGATGGTCTGACAGG+AGG - chr4.2:26771849-26771868 None:intergenic 50.0%
!! GGTCTAGTTGATGGTCTGAC+AGG - chr4.2:26771852-26771871 None:intergenic 50.0%
GATGGTCTGACAGGAGGTTG+TGG - chr4.2:26771843-26771862 None:intergenic 55.0%
GGAAATGGAGCATGCCACAG+CGG + chr4.2:26771050-26771069 MS.gene82359:CDS 55.0%
GGAGTTGCCTGAGACCTCAT+TGG + chr4.2:26771137-26771156 MS.gene82359:CDS 55.0%
GTCTTGGCCAATGAGGTCTC+AGG - chr4.2:26771147-26771166 None:intergenic 55.0%
TAGAAGCAGGAACAAGCCCC+TGG - chr4.2:26772600-26772619 None:intergenic 55.0%
TGATGCTGGTGCGAATGAGG+TGG - chr4.2:26771619-26771638 None:intergenic 55.0%
TGTGGAGCCACCGAAAGTCT+AGG + chr4.2:26768794-26768813 MS.gene82359:intron 55.0%
TTGTCCCAACCACGTCCTCA+TGG + chr4.2:26771790-26771809 MS.gene82359:CDS 55.0%
!! GCTTCTGGTCTTGGCCAATG+AGG - chr4.2:26771154-26771173 None:intergenic 55.0%
CTGACAGGAGGTTGTGGTGG+CGG - chr4.2:26771837-26771856 None:intergenic 60.0%
GAGAGCCTTGCCGCACATTG+TGG - chr4.2:26771329-26771348 None:intergenic 60.0%
GGTCTGACAGGAGGTTGTGG+TGG - chr4.2:26771840-26771859 None:intergenic 60.0%
GCGGCCATGAGGACGTGGTT+GGG - chr4.2:26771797-26771816 None:intergenic 65.0%
TGCGGCCATGAGGACGTGGT+TGG - chr4.2:26771798-26771817 None:intergenic 65.0%
TGCTGCAGCAGCTACCGCTG+TGG - chr4.2:26771067-26771086 None:intergenic 65.0%
!! ACAGGAGGTTGTGGTGGCGG+CGG - chr4.2:26771834-26771853 None:intergenic 65.0%
CGGCTGCGGCCATGAGGACG+TGG - chr4.2:26771802-26771821 None:intergenic 75.0%
!! GGAGGTTGTGGTGGCGGCGG+AGG - chr4.2:26771831-26771850 None:intergenic 75.0%
!! GGTTGTGGTGGCGGCGGAGG+TGG - chr4.2:26771828-26771847 None:intergenic 75.0%
!! TGGTGGCGGCTGCGGCCATG+AGG - chr4.2:26771808-26771827 None:intergenic 75.0%
!! TGTGGTGGCGGCGGAGGTGG+TGG - chr4.2:26771825-26771844 None:intergenic 75.0%
!! GGTGGCGGCGGAGGTGGTGG+CGG - chr4.2:26771822-26771841 None:intergenic 80.0%
!!! GGCGGAGGTGGTGGCGGCTG+CGG - chr4.2:26771816-26771835 None:intergenic 80.0%


Chromosome Type Strat End Strand Name
chr4.2 gene 26768667 26772620 26768667 ID=MS.gene82359
chr4.2 mRNA 26768667 26772620 26768667 ID=MS.gene82359.t1;Parent=MS.gene82359
chr4.2 exon 26768667 26768673 26768667 ID=MS.gene82359.t1.exon1;Parent=MS.gene82359.t1
chr4.2 CDS 26768667 26768673 26768667 ID=cds.MS.gene82359.t1;Parent=MS.gene82359.t1
chr4.2 exon 26770980 26771234 26770980 ID=MS.gene82359.t1.exon2;Parent=MS.gene82359.t1
chr4.2 CDS 26770980 26771234 26770980 ID=cds.MS.gene82359.t1;Parent=MS.gene82359.t1
chr4.2 exon 26771535 26771934 26771535 ID=MS.gene82359.t1.exon3;Parent=MS.gene82359.t1
chr4.2 CDS 26771535 26771934 26771535 ID=cds.MS.gene82359.t1;Parent=MS.gene82359.t1
chr4.2 exon 26772045 26772126 26772045 ID=MS.gene82359.t1.exon4;Parent=MS.gene82359.t1
chr4.2 CDS 26772045 26772126 26772045 ID=cds.MS.gene82359.t1;Parent=MS.gene82359.t1
chr4.2 exon 26772426 26772620 26772426 ID=MS.gene82359.t1.exon5;Parent=MS.gene82359.t1
chr4.2 CDS 26772426 26772620 26772426 ID=cds.MS.gene82359.t1;Parent=MS.gene82359.t1
Gene Sequence

>MS.gene82359

ATGAAAGGTACAAAATTTTGTAGAAAAGAGACGAGAATCTGAAAACAAACATGGAAATGGTTAGAGAGAGAAAGAAGATGGTTGAAAGCATAATTGAGGATGAAGAGGACCCCATGAGTTTCATTAATGTGGAGCCACCGAAAGTCTAGGACGTATCTATTTATGGTTGTTGCTCACAAGAAGAATATGGAGTCAACTTCAATTTTTTTGTTTTTGAATTATATTCATGTATAGATGGAGTTGGTGTATTTGAATTGATCCCATGAAATATATGACATAGTATGTTGCTTTAATTATGCTTATATCATGTTTTGATTATTTGTATAAGGTGTTGTTAGTGCTAAACAAATTACTTTTTAAGTACAATATTTAATGTTTACAAACATGGCATTTTAACCATTTAGTTGGGGGTTTGATTTTCTAATTGTACAAATGAAAAATAGTTGTTGGAAGATGCAACCTCTTACGTACATTTTAGTTAGATATAATGATCGATCTCTACAATTACAATTGTTTATCGGAGAGTCACATTGGTTTTAAGCAAAATATTAAGAGTCATAAGATTGTGCTTTTGATAGATACTTTAATTTTAACTTGTACTATATTTGTTAGCATTTTAAATTTCTAAAATTTTCCCAATTGGATATGTTCATTTACATGATGGTTTACTATTTATAAATAGTTGGGAAACTTTGCCCTATAACATGTACTCATAAGTAATAACTTCAATATCGGATGAAATTTAGGTTCTACGACGGAAAAAATTGATTTTAATTGAAATGATTTTATTTGAAAATAAGTTTAAGATAAAATGATTTAAGTTTATTTGAAGTTCGCATGAAGTGTGTTGAACAATAAACTCGAGTGTCAAAATCATAATTGGTGACGAAAGCTATAAATCTTAGTTTTTATAGACAAAACTAATTCTAATCGGGAACCACCAAGGTCAAACATACAAAAACTAAAATATTCAGAACTATTTTTTACTCTTTCAAAAGCGAATCCAATTATCCAAACATGAAATAGATCTCATCTCATTCTTTACTACAAAAAAAAAAAAAAATAGACGGTTCATATTTCAAATTTACATTTTAAATTCTAATGGACAAAATTATAATATGAAGCGTATCTTCATTCAATGATTAATATTAGTTGATTGTTATGTGGATATTGAGTCCAAAGACTCTTGATCTTATGAACTAGGAATCAGCCAACTCGTGTGGATTTTGAATCAAGCATGTATTTATATGAAGGCATCAAATTATATTAATTTAATAACCATTTTCATCAGATTTTTTCAAGAACAGAGCAAAAAGGATCTGCAATCCTTCTTTATACACATAAATTAATATTTACCCTTTTAGAGATTAAAAGTATTAAATATTCAAAAACCAGTTCTTTAATTTATTCCACATGCCAATGTACATAAAAGTTCAAAGCAATACAATAATTTAAATAAAAAAATCTGACACCCTTTTTTCCTTAGCTGATTTAATTTGATATTTTTTAAATTTTCATCAGAGAAAACTTGATTATAAAATAAGGTCAACAAAGTCAAAATAAGATCCAATGCTTGATGGTAAAAAATCTCTCATGAAGAAAAGAATACTTGTCTTCACCCACAAGGCACTTGAAATCCAAACCAGACAACACATGCAAAGTATATCTAACCTTGCTTTCCCCTCAATAACAACCTATGACTATACTCTACAAAAAGGTTCTCATTTCTTTGGTTTCTTCTCTTTCAAATAAGCACCTACCACTCATCATAATTAAAGCACAAAATTCTTCCTGTTTAGAATGGAGAATTGGCTGAAGCAGATAAGGTTAGTAATTTTCACTTTTCACCTTATAGAAATTTAAGATTGTCACATGTATCATTTATTGTAAATAAATAACACTCATATTTTGCATGAAAGCACATGCCAATTCTTCTTGATATATAAAAATCATTTTGTTTTATATGCTAAATAATTTTTTGTTAGAGAGAAATTAATTGGTGCAGAAGAAGAGAATACAACTGATTTTGGTGGTAGTAGGGTCCAGAAAATACCAATACAGAAAAGTTCATCTTTCAAAGGTGATGTACTGTCTTTCCCTTGCAGACTTTGTTATCAGCATTTTAGGAAAGAAATAAAGTCACAAGATAGCACATATTCTCTTTGACCCAAAAAAGCCATTGAAAATTGAAATACTAAATATAAAAAAATGAATGATGGTTGAAATTAACATGCAATAATTTATAGTTTTTGTTTTTGGCTGAAAACTGAATGAGGTAATTAGTTAATTGACAAATGTCTGCAATTTCTACAGAGAAGAAAAAGGCACATAATTGGTTTCAGAGACAATTTTCAAGGAATATGAGTCATGATTATGATTTTATGGAAATGGAGCATGCCACAGCGGTAGCTGCTGCAGCATTTGCAATTAGTTCACATGTCTCAGAAATTCCACATGAGCAGAAAATGTGGGAGTTGCCTGAGACCTCATTGGCCAAGACCAGAAGCAAAGCCTATGATAAAAAATCTTCATTTTCCCATATAGGTGCTGCATCCAAAAGATTATCAGGTAAATATTTATCTTTTTTTTTTTTTTTTCTTTTAATCTAGTGTTCTCTTCGTGCAACTGCATATACGCTAATCCATCGAGCTGAGTAGAACCACAATGTGCGGCAAGGCTCTCCCTCAAGAGTTTTTAACGTTCTTGTATTCTTAGGGATAAATTCGGACTCACTACCTCTTGGACTGAAAGAGACTTGAATCATCTCATCAAAGCACAATTTGTTACATTATTTATCATTTATTCGCAGCATAATGAGAAAATTAACAAAACTACAATTATGATGAACTCAATATGCATCACTTGTAGGCTCATTTAAAATCACAGATGAACAAGGAAACAAGGTGCCAACAACATCTTCTATTAGAGAAGAAAAAAGGCCTGAAAAGACCACCTCATTCGCACCAGCATCATCAATGACAAAAACTCCTACTTTTGGTGAAAAATCAAAGAGAAATGATGATAAAAAACCGGATATCCTAACACCAAAAAGGACTCCAAGTTTTGGTGGTAAAGATTTTATTAACACTGATGATATACAACCTGAGACCCCAAAACCAAAAATTGTCCCAACCACGTCCTCATGGCCGCAGCCGCCACCACCTCCGCCGCCACCACAACCTCCTGTCAGACCATCAACTAGACCAGGTGCAACAGAAGCAAAAGCAGATACTTGGGAAAGGGAAGAGCTCAAAAAGATCAAAGAAAGGTAGATGCACAAGCATAACAATTGTATATTTCATTAACACTATGAATATTATGCATCTATTATCTATGACTTCATGCATTGATGCAATGAAAATATAATCAATTTGTCAGGTATGAGAAGTTGTTAGAGACAATAGATTCATGGGAAAAGCGGAAGAAGATGAAAGCAAGACGCAAGCTAAACAAGCATGAGGTTAGATTTTCATATTCAGGCCCTGTTTGGATAACCACTTTAATTGAGCGCTTACACACACGTGTTTATCGTATAACTGCTTATGTCTAAGCTATTTCTATATTAAGCGTCAATCATATAATTGCCTAATTATAAGCTGATTAAACTGTTTTCATATAACAGTTTATGAATATGTCATAATCTGTTGTCATAAATTTGCTAAAAAATTGTTTACGCAAATAGATAAGTTCAAATAAACTAATCCAAAATGCTCTTGTTAATTCTCTGATAAAGTGTGGTGACTTTGTGAAAAAATGCAGCAAAGTGAAAATACACGGAAAAGGGAGAAAGCATGGAAAAAATATCAAGATAAGATTAAATATATAGACGAGATTGCAGAAGGAGCAAGAGCACAGTCCGATGAGAGACGAAAGAATGAAACGCTGAAAGCAAAAGATAAGGCAAACATTATTAGAACAACAGGAAAGCTTCCAGGGGCTTGTTCCTGCTTCTAA
Protein sequence

>MS.gene82359.t1

MKEKKKAHNWFQRQFSRNMSHDYDFMEMEHATAVAAAAFAISSHVSEIPHEQKMWELPETSLAKTRSKAYDKKSSFSHIGAASKRLSGSFKITDEQGNKVPTTSSIREEKRPEKTTSFAPASSMTKTPTFGEKSKRNDDKKPDILTPKRTPSFGGKDFINTDDIQPETPKPKIVPTTSSWPQPPPPPPPPQPPVRPSTRPGATEAKADTWEREELKKIKERYEKLLETIDSWEKRKKMKARRKLNKHEQSENTRKREKAWKKYQDKIKYIDEIAEGARAQSDERRKNETLKAKDKANIIRTTGKLPGACSCF