Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene82772.t1 | XP_013450699.1 | 89.1 | 137 | 11 | 2 | 1 | 137 | 3 | 135 | 1.10E-57 | 232.6 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene82772.t1 | P80680 | 50.5 | 95 | 43 | 3 | 43 | 137 | 6 | 96 | 1.7e-19 | 97.1 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene82772.t1 | I3SXR8 | 89.1 | 137 | 11 | 2 | 1 | 137 | 3 | 135 | 7.6e-58 | 232.6 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene052322 | MS.gene82772 | 0.829955 | 3.85E-55 | -1.69E-46 |
| MS.gene053009 | MS.gene82772 | 0.807898 | 4.04E-50 | -1.69E-46 |
| MS.gene057207 | MS.gene82772 | 0.814033 | 1.89E-51 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene82772.t1 | MTR_6g004100 | 89.781 | 137 | 10 | 3 | 1 | 137 | 3 | 135 | 2.45e-84 | 243 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene82772.t1 | AT5G23440 | 47.260 | 146 | 54 | 6 | 11 | 137 | 40 | 181 | 6.38e-33 | 114 |
| MS.gene82772.t1 | AT5G08410 | 52.941 | 119 | 34 | 5 | 38 | 137 | 88 | 203 | 3.11e-31 | 110 |
| MS.gene82772.t1 | AT5G08410 | 52.941 | 119 | 34 | 5 | 38 | 137 | 68 | 183 | 3.12e-31 | 110 |
Find 26 sgRNAs with CRISPR-Local
Find 26 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TCAAACAGAACGTGACTTTC+TGG | 0.206313 | 6.1:+138993 | MS.gene82772:CDS |
| CTCTGCTAATCTTCCTTATA+AGG | 0.228848 | 6.1:+139033 | MS.gene82772:CDS |
| GCAACAAATTCAACCTTATA+AGG | 0.312698 | 6.1:-139046 | None:intergenic |
| CCAAGATTGGAGCGCGTGTT+AGG | 0.324986 | 6.1:+138897 | MS.gene82772:CDS |
| AAGTCACGTTCTGTTTGATT+TGG | 0.360970 | 6.1:-138988 | None:intergenic |
| CCTAACACGCGCTCCAATCT+TGG | 0.382971 | 6.1:-138897 | None:intergenic |
| TGGAGCGCGTGTTAGGGTTA+AGG | 0.393600 | 6.1:+138904 | MS.gene82772:CDS |
| GAGATCAACTTCAGGAACTT+TGG | 0.396637 | 6.1:-138951 | None:intergenic |
| CACTTGTTCAATTGCAACAT+TGG | 0.398273 | 6.1:+138751 | MS.gene82772:CDS |
| AGATCAACTTCAGGAACTTT+GGG | 0.402665 | 6.1:-138950 | None:intergenic |
| TTTGATTTGGCCTTCTCTAC+CGG | 0.427599 | 6.1:-138975 | None:intergenic |
| TCATCATCAACAGCCAAGAT+TGG | 0.450662 | 6.1:+138884 | MS.gene82772:CDS |
| CTTCAGGAACTTTGGGAACA+TGG | 0.459074 | 6.1:-138943 | None:intergenic |
| CAAGATTGGAGCGCGTGTTA+GGG | 0.489458 | 6.1:+138898 | MS.gene82772:CDS |
| CTACCGGTGAGATCAACTTC+AGG | 0.490133 | 6.1:-138959 | None:intergenic |
| TTTGTTGCTGATGATATTCA+AGG | 0.490454 | 6.1:+139061 | MS.gene82772:CDS |
| GGAACATGGTAGACTTTGAG+AGG | 0.538879 | 6.1:-138929 | None:intergenic |
| ACTCAGATAAGATGTGATGT+TGG | 0.560643 | 6.1:+138845 | MS.gene82772:CDS |
| GTTGATCTCACCGGTAGAGA+AGG | 0.561001 | 6.1:+138965 | MS.gene82772:CDS |
| AGATGAGCAACAAACTTGAG+AGG | 0.590824 | 6.1:-139094 | None:intergenic |
| GTTCCTGAAGTTGATCTCAC+CGG | 0.609098 | 6.1:+138956 | MS.gene82772:CDS |
| CAATGTTGCAATTGAACAAG+TGG | 0.609403 | 6.1:-138750 | None:intergenic |
| TTCTTCGTATTCATAAACAA+CGG | 0.618230 | 6.1:-138785 | None:intergenic |
| GTATTCATAAACAACGGCAA+CGG | 0.669143 | 6.1:-138779 | None:intergenic |
| GATGATATTCAAGGTCCTCG+TGG | 0.683280 | 6.1:+139070 | MS.gene82772:CDS |
| ACAAACTTGAGAGGACCACG+AGG | 0.762532 | 6.1:-139085 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| ! | TTCTTCGTATTCATAAACAA+CGG | - | chr6.1:138788-138807 | None:intergenic | 25.0% |
| GCAACAAATTCAACCTTATA+AGG | - | chr6.1:139049-139068 | None:intergenic | 30.0% | |
| TTTGTTGCTGATGATATTCA+AGG | + | chr6.1:139061-139080 | MS.gene82772:CDS | 30.0% | |
| AAGTCACGTTCTGTTTGATT+TGG | - | chr6.1:138991-139010 | None:intergenic | 35.0% | |
| ACTCAGATAAGATGTGATGT+TGG | + | chr6.1:138845-138864 | MS.gene82772:CDS | 35.0% | |
| AGATCAACTTCAGGAACTTT+GGG | - | chr6.1:138953-138972 | None:intergenic | 35.0% | |
| CAATGTTGCAATTGAACAAG+TGG | - | chr6.1:138753-138772 | None:intergenic | 35.0% | |
| CACTTGTTCAATTGCAACAT+TGG | + | chr6.1:138751-138770 | MS.gene82772:CDS | 35.0% | |
| CTCTGCTAATCTTCCTTATA+AGG | + | chr6.1:139033-139052 | MS.gene82772:CDS | 35.0% | |
| GTATTCATAAACAACGGCAA+CGG | - | chr6.1:138782-138801 | None:intergenic | 35.0% | |
| AGATGAGCAACAAACTTGAG+AGG | - | chr6.1:139097-139116 | None:intergenic | 40.0% | |
| GAGATCAACTTCAGGAACTT+TGG | - | chr6.1:138954-138973 | None:intergenic | 40.0% | |
| TCAAACAGAACGTGACTTTC+TGG | + | chr6.1:138993-139012 | MS.gene82772:CDS | 40.0% | |
| TCATCATCAACAGCCAAGAT+TGG | + | chr6.1:138884-138903 | MS.gene82772:CDS | 40.0% | |
| !! | TTTGATTTGGCCTTCTCTAC+CGG | - | chr6.1:138978-138997 | None:intergenic | 40.0% |
| GATGATATTCAAGGTCCTCG+TGG | + | chr6.1:139070-139089 | MS.gene82772:CDS | 45.0% | |
| GGAACATGGTAGACTTTGAG+AGG | - | chr6.1:138932-138951 | None:intergenic | 45.0% | |
| ! | CTTCAGGAACTTTGGGAACA+TGG | - | chr6.1:138946-138965 | None:intergenic | 45.0% |
| ! | GTTCCTGAAGTTGATCTCAC+CGG | + | chr6.1:138956-138975 | MS.gene82772:CDS | 45.0% |
| ACAAACTTGAGAGGACCACG+AGG | - | chr6.1:139088-139107 | None:intergenic | 50.0% | |
| CAAGATTGGAGCGCGTGTTA+GGG | + | chr6.1:138898-138917 | MS.gene82772:CDS | 50.0% | |
| CTACCGGTGAGATCAACTTC+AGG | - | chr6.1:138962-138981 | None:intergenic | 50.0% | |
| GTTGATCTCACCGGTAGAGA+AGG | + | chr6.1:138965-138984 | MS.gene82772:CDS | 50.0% | |
| CCAAGATTGGAGCGCGTGTT+AGG | + | chr6.1:138897-138916 | MS.gene82772:CDS | 55.0% | |
| CCTAACACGCGCTCCAATCT+TGG | - | chr6.1:138900-138919 | None:intergenic | 55.0% | |
| TGGAGCGCGTGTTAGGGTTA+AGG | + | chr6.1:138904-138923 | MS.gene82772:CDS | 55.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr6.1 | gene | 138734 | 139147 | 138734 | ID=MS.gene82772 |
| chr6.1 | mRNA | 138734 | 139147 | 138734 | ID=MS.gene82772.t1;Parent=MS.gene82772 |
| chr6.1 | exon | 138734 | 139147 | 138734 | ID=MS.gene82772.t1.exon1;Parent=MS.gene82772.t1 |
| chr6.1 | CDS | 138734 | 139147 | 138734 | ID=cds.MS.gene82772.t1;Parent=MS.gene82772.t1 |
| Gene Sequence |
| Protein sequence |
