Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene83109.t1 | XP_024634768.1 | 92.2 | 103 | 8 | 0 | 1 | 103 | 288 | 390 | 4.60E-45 | 190.3 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene83109.t1 | Q5SVZ6 | 28.7 | 94 | 67 | 0 | 10 | 103 | 671 | 764 | 6.1e-06 | 51.6 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene83109.t1 | A0A2K3M771 | 81.6 | 103 | 19 | 0 | 1 | 103 | 344 | 446 | 5.5e-40 | 172.9 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene054174 | MS.gene83109 | 0.820833 | 5.57E-53 | -1.69E-46 |
| MS.gene054175 | MS.gene83109 | 0.824959 | 6.08E-54 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene83109.t1 | AT1G19260 | 46.465 | 99 | 53 | 0 | 1 | 99 | 280 | 378 | 5.21e-27 | 103 |
| MS.gene83109.t1 | AT3G29763 | 44.444 | 99 | 55 | 0 | 1 | 99 | 148 | 246 | 5.05e-26 | 100 |
| MS.gene83109.t1 | AT3G29765 | 44.444 | 99 | 55 | 0 | 1 | 99 | 47 | 145 | 6.59e-26 | 100 |
| MS.gene83109.t1 | AT1G41920 | 41.414 | 99 | 58 | 0 | 1 | 99 | 105 | 203 | 1.45e-22 | 90.9 |
| MS.gene83109.t1 | AT2G06541 | 43.434 | 99 | 56 | 0 | 1 | 99 | 213 | 311 | 3.21e-22 | 90.1 |
| MS.gene83109.t1 | AT2G06541 | 43.434 | 99 | 56 | 0 | 1 | 99 | 213 | 311 | 3.22e-22 | 90.1 |
| MS.gene83109.t1 | AT4G09660 | 46.667 | 75 | 40 | 0 | 1 | 75 | 244 | 318 | 1.98e-19 | 82.0 |
| MS.gene83109.t1 | AT3G29450 | 42.029 | 69 | 40 | 0 | 31 | 99 | 194 | 262 | 8.02e-14 | 66.2 |
| MS.gene83109.t1 | AT3G29794 | 34.545 | 110 | 66 | 2 | 1 | 104 | 72 | 181 | 5.44e-13 | 63.5 |
| MS.gene83109.t1 | AT1G42710 | 41.935 | 62 | 36 | 0 | 1 | 62 | 122 | 183 | 6.71e-13 | 62.0 |
Find 15 sgRNAs with CRISPR-Local
Find 17 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| CACTGCTTCCTTAACTCTTA+AGG | 0.350127 | 1.2:-16521834 | MS.gene83109:CDS |
| TACTGCTTCCTTAAGAGTTA+AGG | 0.386962 | 1.2:+16521826 | None:intergenic |
| CTTGATATTTCTAACATTCG+TGG | 0.430795 | 1.2:-16521779 | MS.gene83109:CDS |
| TTAAGATTTGTTGATAAAGT+TGG | 0.449088 | 1.2:-16521899 | MS.gene83109:CDS |
| TTCTCTTGATGCAGTAACTA+AGG | 0.467018 | 1.2:+16521637 | None:intergenic |
| AACATTCGTGGTCAAGGGTA+TGG | 0.468116 | 1.2:-16521767 | MS.gene83109:CDS |
| ATTTCTAACATTCGTGGTCA+AGG | 0.469088 | 1.2:-16521773 | MS.gene83109:CDS |
| TAGAGGACATAGTAAGCATA+AGG | 0.488113 | 1.2:+16521683 | None:intergenic |
| CACGAATGTTAGAAATATCA+AGG | 0.504868 | 1.2:+16521780 | None:intergenic |
| GCAACCGATGAGCAAAATAG+AGG | 0.550001 | 1.2:+16521666 | None:intergenic |
| TTTCTAACATTCGTGGTCAA+GGG | 0.564642 | 1.2:-16521772 | MS.gene83109:CDS |
| TTAGCAATATGAGAGGAGAA+TGG | 0.568120 | 1.2:-16521739 | MS.gene83109:CDS |
| ACTCTTAAGGAAGCAGTATG+TGG | 0.576526 | 1.2:-16521821 | MS.gene83109:CDS |
| GGCAATGTTAGCAATATGAG+AGG | 0.642845 | 1.2:-16521746 | MS.gene83109:CDS |
| AATATGAGAGGAGAATGGAA+TGG | 0.688281 | 1.2:-16521734 | MS.gene83109:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !!! | TTAAGATTTGTTGATAAAGT+TGG | - | chr1.2:16521631-16521650 | MS.gene83109:CDS | 20.0% |
| CACGAATGTTAGAAATATCA+AGG | + | chr1.2:16521753-16521772 | None:intergenic | 30.0% | |
| ! | CTTGATATTTCTAACATTCG+TGG | - | chr1.2:16521751-16521770 | MS.gene83109:CDS | 30.0% |
| !!! | ACAAGAGAGATTTTTTGATG+TGG | - | chr1.2:16521660-16521679 | MS.gene83109:CDS | 30.0% |
| AATATGAGAGGAGAATGGAA+TGG | - | chr1.2:16521796-16521815 | MS.gene83109:CDS | 35.0% | |
| ATTTCTAACATTCGTGGTCA+AGG | - | chr1.2:16521757-16521776 | MS.gene83109:CDS | 35.0% | |
| TACTGCTTCCTTAAGAGTTA+AGG | + | chr1.2:16521707-16521726 | None:intergenic | 35.0% | |
| TAGAGGACATAGTAAGCATA+AGG | + | chr1.2:16521850-16521869 | None:intergenic | 35.0% | |
| TTAGCAATATGAGAGGAGAA+TGG | - | chr1.2:16521791-16521810 | MS.gene83109:CDS | 35.0% | |
| TTCTCTTGATGCAGTAACTA+AGG | + | chr1.2:16521896-16521915 | None:intergenic | 35.0% | |
| TTTCTAACATTCGTGGTCAA+GGG | - | chr1.2:16521758-16521777 | MS.gene83109:CDS | 35.0% | |
| ! | ATGTCCTCTATTTTGCTCAT+CGG | - | chr1.2:16521860-16521879 | MS.gene83109:CDS | 35.0% |
| ACTCTTAAGGAAGCAGTATG+TGG | - | chr1.2:16521709-16521728 | MS.gene83109:CDS | 40.0% | |
| CACTGCTTCCTTAACTCTTA+AGG | - | chr1.2:16521696-16521715 | MS.gene83109:CDS | 40.0% | |
| GGCAATGTTAGCAATATGAG+AGG | - | chr1.2:16521784-16521803 | MS.gene83109:CDS | 40.0% | |
| AACATTCGTGGTCAAGGGTA+TGG | - | chr1.2:16521763-16521782 | MS.gene83109:CDS | 45.0% | |
| GCAACCGATGAGCAAAATAG+AGG | + | chr1.2:16521867-16521886 | None:intergenic | 45.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr1.2 | gene | 16521619 | 16521933 | 16521619 | ID=MS.gene83109 |
| chr1.2 | mRNA | 16521619 | 16521933 | 16521619 | ID=MS.gene83109.t1;Parent=MS.gene83109 |
| chr1.2 | exon | 16521619 | 16521933 | 16521619 | ID=MS.gene83109.t1.exon1;Parent=MS.gene83109.t1 |
| chr1.2 | CDS | 16521619 | 16521933 | 16521619 | ID=cds.MS.gene83109.t1;Parent=MS.gene83109.t1 |
| Gene Sequence |
| Protein sequence |
