AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene83225

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene83225.t1 MTR_2g055940 94.505 364 19 1 1 364 1 363 0.0 707
MS.gene83225.t1 MTR_7g011900 75.207 363 87 3 3 364 10 370 0.0 565
MS.gene83225.t1 MTR_7g011990 68.044 363 110 4 3 364 10 367 0.0 513
MS.gene83225.t1 MTR_7g012020 68.320 363 112 3 3 364 9 369 0.0 512
MS.gene83225.t1 MTR_7g012040 68.595 363 110 4 3 364 9 368 0.0 506
MS.gene83225.t1 MTR_7g012090 62.772 368 129 4 3 364 10 375 4.80e-166 469
MS.gene83225.t1 MTR_7g012070 62.500 368 130 4 3 364 10 375 7.03e-166 468
MS.gene83225.t1 MTR_1g096310 61.202 366 135 5 3 364 10 372 2.59e-163 461
MS.gene83225.t1 MTR_7g012020 71.321 265 73 3 3 266 9 271 1.08e-131 379
MS.gene83225.t1 MTR_1g036460 50.272 368 172 7 3 364 8 370 9.02e-128 371
MS.gene83225.t1 MTR_1g036490 50.543 368 171 8 3 364 8 370 2.04e-127 370
MS.gene83225.t1 MTR_1g036510 51.282 351 162 6 20 364 37 384 2.97e-120 353
MS.gene83225.t1 MTR_7g012020 71.545 246 67 3 3 247 9 252 3.27e-120 348
MS.gene83225.t1 MTR_3g021440 50.568 352 169 5 15 364 22 370 3.39e-117 344
MS.gene83225.t1 MTR_3g021430 49.580 357 175 5 10 364 4 357 6.63e-117 343
MS.gene83225.t1 MTR_3g092900 43.132 364 198 7 3 364 7 363 5.71e-103 308
MS.gene83225.t1 MTR_5g098170 41.011 356 203 7 12 364 12 363 1.57e-89 273
MS.gene83225.t1 MTR_1g050220 44.444 333 129 7 20 347 6 287 4.47e-86 263
MS.gene83225.t1 MTR_0591s0010 39.884 346 202 6 22 364 35 377 2.15e-85 263
MS.gene83225.t1 MTR_6g060290 72.881 177 31 3 3 178 10 170 6.32e-82 247
MS.gene83225.t1 MTR_7g118320 39.306 346 204 6 22 364 20 362 1.59e-81 253
MS.gene83225.t1 MTR_7g118300 38.904 365 211 9 4 364 19 375 6.31e-81 252
MS.gene83225.t1 MTR_1g036510 51.984 252 113 5 20 266 37 285 8.39e-80 246
MS.gene83225.t1 MTR_8g068690 38.484 343 205 6 25 364 37 376 4.18e-79 247
MS.gene83225.t1 MTR_8g068750 37.283 346 211 6 22 364 35 377 3.50e-77 242
MS.gene83225.t1 MTR_8g068870 36.207 348 216 6 20 364 19 363 4.63e-74 234
MS.gene83225.t1 MTR_1g036510 49.784 231 108 5 20 245 37 264 1.24e-67 214
MS.gene83225.t1 MTR_8g068570 38.019 313 162 7 22 331 35 318 5.37e-67 214
MS.gene83225.t1 MTR_8g024160 39.384 292 167 6 77 364 43 328 9.83e-67 214
MS.gene83225.t1 MTR_6g078850 36.338 355 217 7 15 362 32 384 1.06e-66 219
MS.gene83225.t1 MTR_8g068730 35.549 346 185 7 22 364 35 345 1.02e-61 201
MS.gene83225.t1 MTR_2g072790 32.370 346 219 7 10 349 4 340 2.30e-56 188
MS.gene83225.t1 MTR_3g096050 33.871 372 224 11 1 364 1 358 6.28e-55 184
MS.gene83225.t1 MTR_8g068690 37.190 242 148 4 29 268 41 280 8.48e-52 174
MS.gene83225.t1 MTR_3g096070 32.958 355 198 11 1 349 1 321 5.29e-51 173
MS.gene83225.t1 MTR_0017s0100 34.125 337 201 13 35 363 44 367 7.79e-49 169
MS.gene83225.t1 MTR_0079s0070 34.472 322 189 10 35 347 10 318 2.74e-48 166
MS.gene83225.t1 MTR_4g038440 34.472 322 189 10 35 347 44 352 4.98e-48 166
MS.gene83225.t1 MTR_8g068600 35.808 229 143 4 40 266 27 253 6.56e-44 153
MS.gene83225.t1 MTR_8g068740 35.249 261 133 5 22 280 35 261 1.45e-43 152
MS.gene83225.t1 MTR_3g083620 31.646 316 201 7 33 344 38 342 2.97e-43 154
MS.gene83225.t1 MTR_5g074600 30.657 274 175 7 99 363 91 358 4.36e-42 150
MS.gene83225.t1 MTR_5g074680 28.947 342 214 10 35 363 34 359 1.19e-41 149
MS.gene83225.t1 MTR_4g088345 28.698 338 223 7 32 363 30 355 2.29e-39 143
MS.gene83225.t1 MTR_4g088340 28.783 337 224 6 32 363 7 332 5.82e-39 142
MS.gene83225.t1 MTR_3g096050 35.039 254 155 6 16 266 13 259 2.59e-37 135
MS.gene83225.t1 MTR_1g076940 28.438 320 217 6 35 348 33 346 8.44e-37 136
MS.gene83225.t1 MTR_5g074540 33.180 217 134 4 140 348 113 326 1.06e-36 135
MS.gene83225.t1 MTR_5g074580 26.979 341 222 11 35 363 34 359 1.51e-36 135
MS.gene83225.t1 MTR_4g033015 29.042 334 225 7 34 359 32 361 5.77e-35 132
MS.gene83225.t1 MTR_4g033200 28.743 334 226 7 34 359 32 361 5.09e-34 129
MS.gene83225.t1 MTR_7g014570 31.915 235 149 5 135 363 122 351 6.18e-34 129
MS.gene83225.t1 MTR_4g088200 28.660 321 217 7 34 348 32 346 6.47e-34 129
MS.gene83225.t1 MTR_7g024520 33.498 203 131 2 163 362 147 348 8.44e-34 128
MS.gene83225.t1 MTR_4g079160 34.184 196 121 4 159 348 147 340 9.18e-34 128
MS.gene83225.t1 MTR_7g014510 26.316 304 212 5 63 363 62 356 1.08e-33 128
MS.gene83225.t1 MTR_7g024500 26.398 322 217 7 32 348 29 335 1.66e-33 127
MS.gene83225.t1 MTR_4g033150 28.443 334 227 7 34 359 32 361 2.16e-33 127
MS.gene83225.t1 MTR_8g068620 46.789 109 58 0 158 266 2 110 4.84e-33 119
MS.gene83225.t1 MTR_5g074610 29.098 244 139 5 126 363 110 325 5.39e-30 117
MS.gene83225.t1 MTR_4g038440 34.310 239 138 7 35 266 44 270 2.00e-29 114
MS.gene83225.t1 MTR_4g088190 29.193 322 217 7 34 348 32 349 2.92e-29 116
MS.gene83225.t1 MTR_4g033170 30.303 264 159 8 34 288 32 279 1.32e-26 108
MS.gene83225.t1 MTR_4g033085 30.038 263 159 8 34 287 32 278 4.10e-25 104
MS.gene83225.t1 MTR_1g076930 24.790 238 169 5 32 266 7 237 1.52e-20 90.1
MS.gene83225.t1 MTR_6g060280 69.355 62 13 2 219 275 2 62 3.18e-20 84.0
MS.gene83225.t1 MTR_8g068760 38.000 100 60 2 266 364 135 233 1.02e-15 76.3
MS.gene83225.t1 MTR_8g068760 33.600 125 80 3 20 142 20 143 6.60e-11 62.0
MS.gene83225.t1 MTR_0794s0020 25.339 221 158 5 34 251 32 248 1.00e-14 73.6
MS.gene83225.t1 MTR_8g068580 41.304 92 52 2 274 364 43 133 5.15e-14 68.9
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene83225.t1 AT5G54160 44.350 354 191 6 12 364 13 361 1.52e-101 304
MS.gene83225.t1 AT1G77520 38.701 354 207 7 19 364 30 381 4.22e-84 260
MS.gene83225.t1 AT1G51990 38.988 336 199 5 13 344 10 343 2.51e-82 255
MS.gene83225.t1 AT1G51990 38.690 336 200 5 13 344 10 343 5.89e-82 254
MS.gene83225.t1 AT1G77530 40.678 354 200 7 19 364 30 381 5.83e-79 247
MS.gene83225.t1 AT1G33030 39.535 344 195 8 22 358 10 347 9.53e-78 243
MS.gene83225.t1 AT1G63140 38.136 354 209 8 19 364 30 381 1.45e-76 241
MS.gene83225.t1 AT1G63140 38.136 354 209 8 19 364 50 401 1.96e-76 241
MS.gene83225.t1 AT5G53810 38.012 342 201 8 32 364 39 378 8.94e-70 223
MS.gene83225.t1 AT1G21100 38.682 349 199 9 15 354 22 364 1.19e-68 220
MS.gene83225.t1 AT1G21130 38.395 349 200 9 15 354 22 364 3.10e-67 216
MS.gene83225.t1 AT1G21110 38.109 349 201 9 15 354 22 364 8.68e-66 213
MS.gene83225.t1 AT1G76790 37.701 374 209 11 6 364 1 365 7.53e-65 210
MS.gene83225.t1 AT1G21120 37.822 349 202 9 15 354 22 364 1.11e-64 210
MS.gene83225.t1 AT1G21120 37.822 349 202 9 15 354 70 412 4.42e-64 210
MS.gene83225.t1 AT3G53140 35.380 342 197 8 20 348 14 344 1.46e-60 199
MS.gene83225.t1 AT1G62900 42.233 206 116 3 161 364 1 205 1.58e-57 186
MS.gene83225.t1 AT5G37170 33.133 332 181 9 41 364 36 334 1.40e-51 175
MS.gene83225.t1 AT5G37170 41.414 198 112 4 170 364 38 234 2.38e-51 171
MS.gene83225.t1 AT1G33030 39.600 250 145 5 22 269 10 255 8.72e-51 171
MS.gene83225.t1 AT1G33030 39.676 247 143 5 22 266 10 252 5.26e-50 169
MS.gene83225.t1 AT5G37170 41.176 187 106 4 181 364 1 186 4.78e-48 161
MS.gene83225.t1 AT1G63140 36.220 254 155 5 19 266 50 302 7.83e-44 154
MS.gene83225.t1 AT1G77530 37.872 235 139 4 19 247 30 263 4.02e-39 140
MS.gene83225.t1 AT1G21130 37.452 259 149 7 15 266 22 274 1.54e-38 139
MS.gene83225.t1 AT4G35150 30.864 243 150 5 123 348 68 309 8.09e-38 138
MS.gene83225.t1 AT4G35160 33.205 259 157 6 105 348 109 366 1.13e-35 134
MS.gene83225.t1 AT1G63140 34.894 235 146 5 19 247 50 283 2.23e-33 125

Find 76 sgRNAs with CRISPR-Local

Find 85 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GGTAGTACTTTGAGAGTTTA+TGG 0.138774 2.4:-34744592 MS.gene83225:CDS
TCCCTTTATTGATGGGTATT+TGG 0.140968 2.4:+34744120 None:intergenic
GACGCATCTCGATTCATTTA+TGG 0.185909 2.4:-34743622 MS.gene83225:CDS
TGAAAATGATGGTGGTTATT+TGG 0.190360 2.4:-34744536 MS.gene83225:CDS
GACCTATTCAAGAAAGTTAA+TGG 0.210657 2.4:-34744325 MS.gene83225:CDS
CGAGATGCGTCTGTTGGTTT+TGG 0.210850 2.4:+34743634 None:intergenic
TTTGAAAGAATATGAAAGTT+TGG 0.223106 2.4:-34743554 MS.gene83225:CDS
TGCCAAATACCCATCAATAA+AGG 0.282531 2.4:-34744122 MS.gene83225:CDS
AAGACACGATCGAGTCTATT+TGG 0.300123 2.4:+34744673 None:intergenic
GTTTATGGGGTCACTTCTTC+TGG 0.301463 2.4:-34744577 MS.gene83225:CDS
GAAAATGATGGTGGTTATTT+GGG 0.319757 2.4:-34744535 MS.gene83225:CDS
TTCCATTAACTTTCTTGAAT+AGG 0.343440 2.4:+34744323 None:intergenic
TTGAAAGAATATGAAAGTTT+GGG 0.349066 2.4:-34743553 MS.gene83225:CDS
CACACTGACCTTAAGTAGTA+TGG 0.352819 2.4:+34743897 None:intergenic
AAAGCCTTGTGACAAGTAAA+TGG 0.354130 2.4:+34744502 None:intergenic
ACTTGTCACAAGGCTTTGTT+TGG 0.367785 2.4:-34744496 MS.gene83225:CDS
TTTAGAGTGTATGCCATAAC+TGG 0.370585 2.4:-34743753 MS.gene83225:intron
CATTTAGGGATCCAACATAT+TGG 0.374317 2.4:-34743955 MS.gene83225:intron
ACACTCTCTGCTATGGTGTT+AGG 0.375320 2.4:-34744832 MS.gene83225:CDS
GGTAAGTCGGAGTGTTGATT+TGG 0.380815 2.4:+34744694 None:intergenic
GTAGTACTTTGAGAGTTTAT+GGG 0.390682 2.4:-34744591 MS.gene83225:CDS
AAATTGATTCCCTTTATTGA+TGG 0.391826 2.4:+34744112 None:intergenic
GCCAAATACCCATCAATAAA+GGG 0.393194 2.4:-34744121 MS.gene83225:CDS
CACAAAGCTTTACCACCAAA+TGG 0.394075 2.4:-34743694 MS.gene83225:CDS
ATCATCGTCGTTGGTGCGAT+TGG 0.398696 2.4:+34744615 None:intergenic
TATGGTGTTAGGTTCTGTTA+TGG 0.402609 2.4:-34744821 MS.gene83225:CDS
ATGATAATGACCTTCCCATT+TGG 0.411262 2.4:+34743679 None:intergenic
GAAAGTTTGGGTAAACGATC+TGG 0.423215 2.4:-34743541 MS.gene83225:CDS
AGGAGATGCCATACTACTTA+AGG 0.430145 2.4:-34743905 MS.gene83225:intron
AATTGATTCCCTTTATTGAT+GGG 0.443973 2.4:+34744113 None:intergenic
ATCATTGCTAGCAGCAATGA+TGG 0.448242 2.4:-34744751 MS.gene83225:CDS
ATATCTATGTATGAGTACTT+TGG 0.452220 2.4:-34744301 MS.gene83225:CDS
ATTGTTCATCCGACCAGTTA+TGG 0.467416 2.4:+34743740 None:intergenic
GGAACTCACACCTCACAAAC+AGG 0.473367 2.4:-34744868 MS.gene83225:CDS
CAAGGAAGTAATAGTGAATG+AGG 0.480422 2.4:-34744353 MS.gene83225:CDS
ATCTCGATTCATTTATGGTA+TGG 0.481444 2.4:-34743617 MS.gene83225:CDS
TCTTGAGGGAGTATCAACTA+TGG 0.481625 2.4:-34744188 MS.gene83225:CDS
GTATTGTAGGTCTAATTTCA+AGG 0.496292 2.4:-34744371 MS.gene83225:intron
TTTCTCCATTCTCGAAGTCA+TGG 0.501603 2.4:-34743488 MS.gene83225:CDS
AAGTTCCATGACTTCGAGAA+TGG 0.503084 2.4:+34743483 None:intergenic
AACATCATGTTTCTCACAGT+TGG 0.505124 2.4:-34743592 MS.gene83225:CDS
ATCATGTTTCTCACAGTTGG+TGG 0.507213 2.4:-34743589 MS.gene83225:CDS
AATCGCACCAACGACGATGA+TGG 0.508623 2.4:-34744613 MS.gene83225:CDS
GACGAATACAAAGGTCTTGA+GGG 0.512330 2.4:-34744202 MS.gene83225:CDS
GTATCAACTATGGTTGATGT+AGG 0.524001 2.4:-34744178 MS.gene83225:CDS
ACAAAGCTTTACCACCAAAT+GGG 0.525688 2.4:-34743693 MS.gene83225:CDS
TGTTTCTCACAGTTGGTGGA+AGG 0.527248 2.4:-34743585 MS.gene83225:CDS
CACGCCATTTACTTGTCACA+AGG 0.531458 2.4:-34744506 MS.gene83225:CDS
ACATGCTTCCTCCAATATGT+TGG 0.534854 2.4:+34743944 None:intergenic
GTTTCTCACAGTTGGTGGAA+GGG 0.541114 2.4:-34743584 MS.gene83225:CDS
ATAATGACCTTCCCATTTGG+TGG 0.542614 2.4:+34743682 None:intergenic
ATTGCAGCATTAAGAACTGC+AGG 0.551711 2.4:+34744793 None:intergenic
TTTGTTGATGATGAAAATGA+TGG 0.552091 2.4:-34744547 MS.gene83225:CDS
ATCGAGTCTATTTGGTAAGT+CGG 0.554297 2.4:+34744681 None:intergenic
TTAGGGATCCAACATATTGG+AGG 0.566339 2.4:-34743952 MS.gene83225:intron
ATGAATCGAGATGCGTCTGT+TGG 0.569983 2.4:+34743628 None:intergenic
TGACGAATACAAAGGTCTTG+AGG 0.572199 2.4:-34744203 MS.gene83225:CDS
ACTACTGTCACCTGTTTGTG+AGG 0.573457 2.4:+34744858 None:intergenic
GAAAATGAACAATAATGTTG+TGG 0.578225 2.4:-34744890 None:intergenic
AAAATGAACAATAATGTTGT+GGG 0.579380 2.4:-34744889 MS.gene83225:CDS
TAGTGATACACTCTCTGCTA+TGG 0.581019 2.4:-34744839 MS.gene83225:CDS
GTTGATGTAGGAGGTGGCAG+TGG 0.588462 2.4:-34744166 MS.gene83225:CDS
TAGTACTTTGAGAGTTTATG+GGG 0.589839 2.4:-34744590 MS.gene83225:CDS
GAAAATGCACCACCTACTCC+AGG 0.597174 2.4:-34744070 MS.gene83225:intron
GTTGATGATGAAAATGATGG+TGG 0.599361 2.4:-34744544 MS.gene83225:CDS
AGTAGTATGGCATCTCCTTG+TGG 0.601193 2.4:+34743910 None:intergenic
AGGAATAGGTTACCTGGAGT+AGG 0.602035 2.4:+34744058 None:intergenic
TCAACTATGGTTGATGTAGG+AGG 0.605473 2.4:-34744175 MS.gene83225:CDS
ACTATGGTTGATGTAGGAGG+TGG 0.606248 2.4:-34744172 MS.gene83225:CDS
AATAGGTTACCTGGAGTAGG+TGG 0.622764 2.4:+34744061 None:intergenic
ACAAGGCTTTGTTTGGAATG+TGG 0.625990 2.4:-34744489 MS.gene83225:intron
AGCTTTACCACCAAATGGGA+AGG 0.626543 2.4:-34743689 MS.gene83225:CDS
AGTACTACCATCATCGTCGT+TGG 0.626740 2.4:+34744606 None:intergenic
GAGTGTATGCCATAACTGGT+CGG 0.668148 2.4:-34743749 MS.gene83225:CDS
AGAATCGTTGACGAATACAA+AGG 0.672277 2.4:-34744211 MS.gene83225:CDS
ATGTTTGAAAGTGTTCCACA+AGG 0.704402 2.4:-34743925 MS.gene83225:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AAAAATTAAATTTGTATTGT+AGG - chr2.4:34743976-34743995 MS.gene83225:intron 10.0%
!! AAAATTACCAAATTTCATTT+AGG - chr2.4:34744390-34744409 MS.gene83225:intron 15.0%
!! AAATTACCAAATTTCATTTA+GGG - chr2.4:34744391-34744410 MS.gene83225:intron 15.0%
!! TATATATAGAAAGTAATCAT+TGG + chr2.4:34744338-34744357 None:intergenic 15.0%
!! TAATCATTGGATATAACTTT+AGG + chr2.4:34744325-34744344 None:intergenic 20.0%
!! TTGAAAGAATATGAAAGTTT+GGG - chr2.4:34744807-34744826 MS.gene83225:CDS 20.0%
!! TTTGAAAGAATATGAAAGTT+TGG - chr2.4:34744806-34744825 MS.gene83225:CDS 20.0%
!!! TATATATCAACATTTTTGCA+TGG + chr2.4:34743954-34743973 None:intergenic 20.0%
! ATATCTATGTATGAGTACTT+TGG - chr2.4:34744059-34744078 MS.gene83225:intron 25.0%
! TTCCATTAACTTTCTTGAAT+AGG + chr2.4:34744040-34744059 None:intergenic 25.0%
!! AAATTGATTCCCTTTATTGA+TGG + chr2.4:34744251-34744270 None:intergenic 25.0%
!! AATTGATTCCCTTTATTGAT+GGG + chr2.4:34744250-34744269 None:intergenic 25.0%
!! TTGGATATAACTTTAGGAAT+AGG + chr2.4:34744319-34744338 None:intergenic 25.0%
!!! TTTGTTGATGATGAAAATGA+TGG - chr2.4:34743813-34743832 MS.gene83225:intron 25.0%
ATCTCGATTCATTTATGGTA+TGG - chr2.4:34744743-34744762 MS.gene83225:CDS 30.0%
GACCTATTCAAGAAAGTTAA+TGG - chr2.4:34744035-34744054 MS.gene83225:intron 30.0%
GTATTGTAGGTCTAATTTCA+AGG - chr2.4:34743989-34744008 MS.gene83225:intron 30.0%
TTGGATCCCTAAATGAAATT+TGG + chr2.4:34744400-34744419 None:intergenic 30.0%
! GTAGTACTTTGAGAGTTTAT+GGG - chr2.4:34743769-34743788 MS.gene83225:intron 30.0%
! TAGTACTTTGAGAGTTTATG+GGG - chr2.4:34743770-34743789 MS.gene83225:intron 30.0%
!! GAAAATGATGGTGGTTATTT+GGG - chr2.4:34743825-34743844 MS.gene83225:intron 30.0%
!! TGAAAATGATGGTGGTTATT+TGG - chr2.4:34743824-34743843 MS.gene83225:intron 30.0%
AACATCATGTTTCTCACAGT+TGG - chr2.4:34744768-34744787 MS.gene83225:CDS 35.0%
ACAAAGCTTTACCACCAAAT+GGG - chr2.4:34744667-34744686 MS.gene83225:CDS 35.0%
AGAATCGTTGACGAATACAA+AGG - chr2.4:34744149-34744168 MS.gene83225:CDS 35.0%
ATCGAGTCTATTTGGTAAGT+CGG + chr2.4:34743682-34743701 None:intergenic 35.0%
CAAGGAAGTAATAGTGAATG+AGG - chr2.4:34744007-34744026 MS.gene83225:intron 35.0%
CATTTAGGGATCCAACATAT+TGG - chr2.4:34744405-34744424 MS.gene83225:intron 35.0%
GCCAAATACCCATCAATAAA+GGG - chr2.4:34744239-34744258 MS.gene83225:CDS 35.0%
TGCCAAATACCCATCAATAA+AGG - chr2.4:34744238-34744257 MS.gene83225:CDS 35.0%
! AAAGCCTTGTGACAAGTAAA+TGG + chr2.4:34743861-34743880 None:intergenic 35.0%
! AACTTTAGGAATAGGTTACC+TGG + chr2.4:34744311-34744330 None:intergenic 35.0%
! ATGATAATGACCTTCCCATT+TGG + chr2.4:34744684-34744703 None:intergenic 35.0%
! GGTAGTACTTTGAGAGTTTA+TGG - chr2.4:34743768-34743787 MS.gene83225:intron 35.0%
! GTATCAACTATGGTTGATGT+AGG - chr2.4:34744182-34744201 MS.gene83225:CDS 35.0%
! TATGGTGTTAGGTTCTGTTA+TGG - chr2.4:34743539-34743558 MS.gene83225:CDS 35.0%
! TCCCTTTATTGATGGGTATT+TGG + chr2.4:34744243-34744262 None:intergenic 35.0%
! TTTAGAGTGTATGCCATAAC+TGG - chr2.4:34744607-34744626 MS.gene83225:CDS 35.0%
!! ATGTTTGAAAGTGTTCCACA+AGG - chr2.4:34744435-34744454 MS.gene83225:intron 35.0%
!! GTTGATGATGAAAATGATGG+TGG - chr2.4:34743816-34743835 MS.gene83225:intron 35.0%
AAGACACGATCGAGTCTATT+TGG + chr2.4:34743690-34743709 None:intergenic 40.0%
AAGTTCCATGACTTCGAGAA+TGG + chr2.4:34744880-34744899 None:intergenic 40.0%
ACATGCTTCCTCCAATATGT+TGG + chr2.4:34744419-34744438 None:intergenic 40.0%
AGGAGATGCCATACTACTTA+AGG - chr2.4:34744455-34744474 MS.gene83225:intron 40.0%
ATAATGACCTTCCCATTTGG+TGG + chr2.4:34744681-34744700 None:intergenic 40.0%
ATCATGTTTCTCACAGTTGG+TGG - chr2.4:34744771-34744790 MS.gene83225:CDS 40.0%
ATCATTGCTAGCAGCAATGA+TGG - chr2.4:34743609-34743628 MS.gene83225:CDS 40.0%
ATTGCAGCATTAAGAACTGC+AGG + chr2.4:34743570-34743589 None:intergenic 40.0%
ATTGTTCATCCGACCAGTTA+TGG + chr2.4:34744623-34744642 None:intergenic 40.0%
CACAAAGCTTTACCACCAAA+TGG - chr2.4:34744666-34744685 MS.gene83225:CDS 40.0%
CACACTGACCTTAAGTAGTA+TGG + chr2.4:34744466-34744485 None:intergenic 40.0%
GAAAGTTTGGGTAAACGATC+TGG - chr2.4:34744819-34744838 MS.gene83225:CDS 40.0%
GACGAATACAAAGGTCTTGA+GGG - chr2.4:34744158-34744177 MS.gene83225:CDS 40.0%
GACGCATCTCGATTCATTTA+TGG - chr2.4:34744738-34744757 MS.gene83225:CDS 40.0%
TAGTGATACACTCTCTGCTA+TGG - chr2.4:34743521-34743540 MS.gene83225:CDS 40.0%
TGACGAATACAAAGGTCTTG+AGG - chr2.4:34744157-34744176 MS.gene83225:CDS 40.0%
TTAGGGATCCAACATATTGG+AGG - chr2.4:34744408-34744427 MS.gene83225:intron 40.0%
TTTCTCCATTCTCGAAGTCA+TGG - chr2.4:34744872-34744891 MS.gene83225:CDS 40.0%
! GGTGCATTTTCAATGACTTG+AGG + chr2.4:34744281-34744300 None:intergenic 40.0%
! TCAACTATGGTTGATGTAGG+AGG - chr2.4:34744185-34744204 MS.gene83225:CDS 40.0%
!! ACAAGGCTTTGTTTGGAATG+TGG - chr2.4:34743871-34743890 MS.gene83225:intron 40.0%
!! ACTTGTCACAAGGCTTTGTT+TGG - chr2.4:34743864-34743883 MS.gene83225:intron 40.0%
!! TCTTGAGGGAGTATCAACTA+TGG - chr2.4:34744172-34744191 MS.gene83225:CDS 40.0%
!!! TCTGTTGGTTTTGGAACATC+TGG + chr2.4:34744720-34744739 None:intergenic 40.0%
AATAGGTTACCTGGAGTAGG+TGG + chr2.4:34744302-34744321 None:intergenic 45.0%
ACACTCTCTGCTATGGTGTT+AGG - chr2.4:34743528-34743547 MS.gene83225:CDS 45.0%
ACTACTGTCACCTGTTTGTG+AGG + chr2.4:34743505-34743524 None:intergenic 45.0%
AGCTTTACCACCAAATGGGA+AGG - chr2.4:34744671-34744690 MS.gene83225:CDS 45.0%
AGGAATAGGTTACCTGGAGT+AGG + chr2.4:34744305-34744324 None:intergenic 45.0%
AGTACTACCATCATCGTCGT+TGG + chr2.4:34743757-34743776 None:intergenic 45.0%
AGTAGTATGGCATCTCCTTG+TGG + chr2.4:34744453-34744472 None:intergenic 45.0%
ATGAATCGAGATGCGTCTGT+TGG + chr2.4:34744735-34744754 None:intergenic 45.0%
CACGCCATTTACTTGTCACA+AGG - chr2.4:34743854-34743873 MS.gene83225:intron 45.0%
GAGTGTATGCCATAACTGGT+CGG - chr2.4:34744611-34744630 MS.gene83225:CDS 45.0%
GTTTATGGGGTCACTTCTTC+TGG - chr2.4:34743783-34743802 MS.gene83225:intron 45.0%
! ACTATGGTTGATGTAGGAGG+TGG - chr2.4:34744188-34744207 MS.gene83225:CDS 45.0%
!! GGTAAGTCGGAGTGTTGATT+TGG + chr2.4:34743669-34743688 None:intergenic 45.0%
!! GTTTCTCACAGTTGGTGGAA+GGG - chr2.4:34744776-34744795 MS.gene83225:CDS 45.0%
!! TGTTTCTCACAGTTGGTGGA+AGG - chr2.4:34744775-34744794 MS.gene83225:CDS 45.0%
AATCGCACCAACGACGATGA+TGG - chr2.4:34743747-34743766 MS.gene83225:CDS 50.0%
ATCATCGTCGTTGGTGCGAT+TGG + chr2.4:34743748-34743767 None:intergenic 50.0%
GAAAATGCACCACCTACTCC+AGG - chr2.4:34744290-34744309 MS.gene83225:CDS 50.0%
GGAACTCACACCTCACAAAC+AGG - chr2.4:34743492-34743511 MS.gene83225:CDS 50.0%
!!! CGAGATGCGTCTGTTGGTTT+TGG + chr2.4:34744729-34744748 None:intergenic 50.0%
GTTGATGTAGGAGGTGGCAG+TGG - chr2.4:34744194-34744213 MS.gene83225:CDS 55.0%


Chromosome Type Strat End Strand Name
chr2.4 gene 34743474 34744908 34743474 ID=MS.gene83225
chr2.4 mRNA 34743474 34744908 34743474 ID=MS.gene83225.t1;Parent=MS.gene83225
chr2.4 exon 34744490 34744908 34744490 ID=MS.gene83225.t1.exon1;Parent=MS.gene83225.t1
chr2.4 CDS 34744490 34744908 34744490 ID=cds.MS.gene83225.t1;Parent=MS.gene83225.t1
chr2.4 exon 34744071 34744384 34744071 ID=MS.gene83225.t1.exon2;Parent=MS.gene83225.t1
chr2.4 CDS 34744071 34744384 34744071 ID=cds.MS.gene83225.t1;Parent=MS.gene83225.t1
chr2.4 exon 34743906 34743970 34743906 ID=MS.gene83225.t1.exon3;Parent=MS.gene83225.t1
chr2.4 CDS 34743906 34743970 34743906 ID=cds.MS.gene83225.t1;Parent=MS.gene83225.t1
chr2.4 exon 34743474 34743770 34743474 ID=MS.gene83225.t1.exon4;Parent=MS.gene83225.t1
chr2.4 CDS 34743474 34743770 34743474 ID=cds.MS.gene83225.t1;Parent=MS.gene83225.t1
Gene Sequence

>MS.gene83225

ATGAACAATAATGTTGTGGGAACTCACACCTCACAAACAGGTGACAGTAGTGATACACTCTCTGCTATGGTGTTAGGTTCTGTTATGGTTCTTCCTGCAGTTCTTAATGCTGCAATTGAGCTCAAATTATTTGATATCATTGCTAGCAGCAATGATGGATTTATGTCAGTCTTTGAAATTGCTTCAAAGTTACCAAATCAACACTCCGACTTACCAAATAGACTCGATCGTGTCTTGTGCGTGCTTGCTAGCTACTCTCTTCTTTCTGTTTCCAATCGCACCAACGACGATGATGGTAGTACTTTGAGAGTTTATGGGGTCACTTCTTCTGGCAAATACTTTGTTGATGATGAAAATGATGGTGGTTATTTGGGATCATTCACGCCATTTACTTGTCACAAGGCTTTGTTTGGAATGTGGTAAGTATTTCTAATTGATTAATTTGCATGAAATCTTGATCTATATGTTGTTAATAGTCCATGCAAAAATGTTGATATATAAAAAAAATTAAATTTGTATTGTAGGTCTAATTTCAAGGAAGTAATAGTGAATGAGGAGATTGACCTATTCAAGAAAGTTAATGGAATATCTATGTATGAGTACTTTGGAAAAGATTCACAAATAAACAAAATATTTAACAAATCAATGACTGATACATGCAATGTTGAGATTAGAAGAATCGTTGACGAATACAAAGGTCTTGAGGGAGTATCAACTATGGTTGATGTAGGAGGTGGCAGTGGACAAAGTCTCAAAACAATTATTGCCAAATACCCATCAATAAAGGGAATCAATTTTGATCTTCCTCAAGTCATTGAAAATGCACCACCTACTCCAGGTAACCTATTCCTAAAGTTATATCCAATGATTACTTTCTATATATATTTTGAACTTTCTTAAGAGTTTAGATAAATTCAAAATTACCAAATTTCATTTAGGGATCCAACATATTGGAGGAAGCATGTTTGAAAGTGTTCCACAAGGAGATGCCATACTACTTAAGGTCAGTGTGTTGATGAAATAATTTAATTTATGAAACATTTATTTGCTTCAATTACAAGAATAACTTCATTTATTATCTACAAGAAATATAGTGCTTACAAAATTATTTTGTATTGAATCTCTCTCATTGTTTTAGAGTGTATGCCATAACTGGTCGGATGAACAATGCATACAAATTTTAAGCAATTGTCACAAAGCTTTACCACCAAATGGGAAGGTCATTATCATCGAGCTCGCAAAACCAGATGTTCCAAAACCAACAGACGCATCTCGATTCATTTATGGTATGGACAACATCATGTTTCTCACAGTTGGTGGAAGGGAAAGAACTTTGAAAGAATATGAAAGTTTGGGTAAACGATCTGGATTTGTTAAGTTTCAAGCTGCTTGTCGTGTTTTCTCCATTCTCGAAGTCATGGAACTTTACAAATAA
Protein sequence

>MS.gene83225.t1

MNNNVVGTHTSQTGDSSDTLSAMVLGSVMVLPAVLNAAIELKLFDIIASSNDGFMSVFEIASKLPNQHSDLPNRLDRVLCVLASYSLLSVSNRTNDDDGSTLRVYGVTSSGKYFVDDENDGGYLGSFTPFTCHKALFGMWSNFKEVIVNEEIDLFKKVNGISMYEYFGKDSQINKIFNKSMTDTCNVEIRRIVDEYKGLEGVSTMVDVGGGSGQSLKTIIAKYPSIKGINFDLPQVIENAPPTPGIQHIGGSMFESVPQGDAILLKSVCHNWSDEQCIQILSNCHKALPPNGKVIIIELAKPDVPKPTDASRFIYGMDNIMFLTVGGRERTLKEYESLGKRSGFVKFQAACRVFSILEVMELYK