Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene058381.t1 | XP_013445244.1 | 92.1 | 152 | 12 | 0 | 1 | 152 | 1 | 152 | 9.30E-79 | 302.8 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene058381.t1 | Q9SSK5 | 34.0 | 150 | 96 | 3 | 3 | 150 | 7 | 155 | 8.9e-22 | 104.8 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene058381.t1 | A0A072U0G0 | 92.1 | 152 | 12 | 0 | 1 | 152 | 1 | 152 | 6.7e-79 | 302.8 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene04932 | MS.gene058381 | 0.806905 | 6.56E-50 | -1.69E-46 |
| MS.gene049549 | MS.gene058381 | 0.81337 | 2.65E-51 | -1.69E-46 |
| MS.gene052211 | MS.gene058381 | 0.817359 | 3.44E-52 | -1.69E-46 |
| MS.gene05240 | MS.gene058381 | 0.817838 | 2.68E-52 | -1.69E-46 |
| MS.gene054146 | MS.gene058381 | 0.804308 | 2.30E-49 | -1.69E-46 |
| MS.gene054277 | MS.gene058381 | 0.825336 | 4.95E-54 | -1.69E-46 |
| MS.gene054742 | MS.gene058381 | 0.812091 | 5.05E-51 | -1.69E-46 |
| MS.gene056590 | MS.gene058381 | 0.806622 | 7.52E-50 | -1.69E-46 |
| MS.gene056591 | MS.gene058381 | 0.805131 | 1.55E-49 | -1.69E-46 |
| MS.gene056697 | MS.gene058381 | 0.810482 | 1.13E-50 | -1.69E-46 |
| MS.gene056920 | MS.gene058381 | 0.806353 | 8.57E-50 | -1.69E-46 |
| MS.gene057261 | MS.gene058381 | 0.807802 | 4.23E-50 | -1.69E-46 |
| MS.gene058381 | MS.gene058450 | 0.803167 | 3.97E-49 | -1.69E-46 |
| MS.gene058381 | MS.gene060087 | 0.838938 | 2.14E-57 | -1.69E-46 |
| MS.gene058381 | MS.gene060358 | 0.823852 | 1.11E-53 | -1.69E-46 |
| MS.gene058381 | MS.gene063148 | 0.817637 | 2.97E-52 | -1.69E-46 |
| MS.gene058381 | MS.gene063436 | 0.820201 | 7.77E-53 | -1.69E-46 |
| MS.gene058381 | MS.gene063988 | 0.823195 | 1.58E-53 | -1.69E-46 |
| MS.gene058381 | MS.gene065466 | 0.829209 | 5.85E-55 | -1.69E-46 |
| MS.gene058381 | MS.gene065724 | 0.805641 | 1.21E-49 | -1.69E-46 |
| MS.gene058381 | MS.gene06593 | 0.818165 | 2.26E-52 | -1.69E-46 |
| MS.gene058381 | MS.gene065973 | 0.804925 | 1.71E-49 | -1.69E-46 |
| MS.gene058381 | MS.gene066417 | 0.815198 | 1.05E-51 | -1.69E-46 |
| MS.gene058381 | MS.gene06756 | 0.830566 | 2.73E-55 | -1.69E-46 |
| MS.gene058381 | MS.gene06805 | 0.81786 | 2.65E-52 | -1.69E-46 |
| MS.gene058381 | MS.gene068448 | 0.809951 | 1.47E-50 | -1.69E-46 |
| MS.gene058381 | MS.gene068449 | 0.83135 | 1.75E-55 | -1.69E-46 |
| MS.gene058381 | MS.gene069482 | 0.809587 | 1.76E-50 | -1.69E-46 |
| MS.gene058381 | MS.gene071840 | 0.804557 | 2.04E-49 | -1.69E-46 |
| MS.gene058381 | MS.gene072293 | 0.824932 | 6.17E-54 | -1.69E-46 |
| MS.gene058381 | MS.gene07241 | 0.806585 | 7.66E-50 | -1.69E-46 |
| MS.gene058381 | MS.gene073526 | 0.82953 | 4.89E-55 | -1.69E-46 |
| MS.gene058381 | MS.gene07839 | 0.802048 | 6.75E-49 | -1.69E-46 |
| MS.gene058381 | MS.gene08354 | 0.809788 | 1.59E-50 | -1.69E-46 |
| MS.gene058381 | MS.gene22157 | 0.801016 | 1.10E-48 | -1.69E-46 |
| MS.gene058381 | MS.gene24659 | 0.806965 | 6.37E-50 | -1.69E-46 |
| MS.gene058381 | MS.gene24881 | 0.813126 | 3.00E-51 | -1.69E-46 |
| MS.gene058381 | MS.gene25652 | 0.801632 | 8.22E-49 | -1.69E-46 |
| MS.gene058381 | MS.gene26296 | 0.800374 | 1.48E-48 | -1.69E-46 |
| MS.gene058381 | MS.gene26475 | 0.803976 | 2.70E-49 | -1.69E-46 |
| MS.gene058381 | MS.gene28548 | 0.808898 | 2.47E-50 | -1.69E-46 |
| MS.gene058381 | MS.gene28594 | 0.821633 | 3.64E-53 | -1.69E-46 |
| MS.gene058381 | MS.gene29888 | 0.811014 | 8.65E-51 | -1.69E-46 |
| MS.gene058381 | MS.gene33334 | 0.803801 | 2.93E-49 | -1.69E-46 |
| MS.gene058381 | MS.gene33445 | 0.80318 | 3.94E-49 | -1.69E-46 |
| MS.gene058381 | MS.gene33490 | 0.808043 | 3.76E-50 | -1.69E-46 |
| MS.gene058381 | MS.gene34968 | 0.814014 | 1.91E-51 | -1.69E-46 |
| MS.gene058381 | MS.gene38435 | 0.825646 | 4.18E-54 | -1.69E-46 |
| MS.gene058381 | MS.gene41490 | 0.813825 | 2.10E-51 | -1.69E-46 |
| MS.gene058381 | MS.gene42005 | 0.885281 | 9.31E-72 | -1.69E-46 |
| MS.gene058381 | MS.gene44958 | 0.802353 | 5.84E-49 | -1.69E-46 |
| MS.gene058381 | MS.gene45228 | 0.807161 | 5.79E-50 | -1.69E-46 |
| MS.gene058381 | MS.gene46197 | 0.805442 | 1.33E-49 | -1.69E-46 |
| MS.gene058381 | MS.gene46198 | 0.807511 | 4.88E-50 | -1.69E-46 |
| MS.gene058381 | MS.gene46369 | 0.817779 | 2.76E-52 | -1.69E-46 |
| MS.gene058381 | MS.gene47719 | 0.821399 | 4.12E-53 | -1.69E-46 |
| MS.gene058381 | MS.gene47841 | 0.800621 | 1.32E-48 | -1.69E-46 |
| MS.gene058381 | MS.gene48023 | 0.821349 | 4.23E-53 | -1.69E-46 |
| MS.gene058381 | MS.gene48885 | 0.823399 | 1.41E-53 | -1.69E-46 |
| MS.gene058381 | MS.gene50073 | 0.808266 | 3.37E-50 | -1.69E-46 |
| MS.gene058381 | MS.gene53436 | 0.800182 | 1.63E-48 | -1.69E-46 |
| MS.gene058381 | MS.gene54252 | 0.824536 | 7.66E-54 | -1.69E-46 |
| MS.gene058381 | MS.gene54253 | 0.807247 | 5.55E-50 | -1.69E-46 |
| MS.gene058381 | MS.gene55012 | 0.833639 | 4.75E-56 | -1.69E-46 |
| MS.gene058381 | MS.gene56554 | 0.822343 | 2.49E-53 | -1.69E-46 |
| MS.gene058381 | MS.gene56765 | 0.814582 | 1.43E-51 | -1.69E-46 |
| MS.gene058381 | MS.gene58646 | 0.806681 | 7.31E-50 | -1.69E-46 |
| MS.gene058381 | MS.gene59333 | 0.813311 | 2.73E-51 | -1.69E-46 |
| MS.gene058381 | MS.gene59415 | 0.800938 | 1.14E-48 | -1.69E-46 |
| MS.gene058381 | MS.gene59850 | 0.918374 | 1.69E-86 | -1.69E-46 |
| MS.gene058381 | MS.gene60577 | 0.829987 | 3.79E-55 | -1.69E-46 |
| MS.gene058381 | MS.gene60838 | 0.808802 | 2.59E-50 | -1.69E-46 |
| MS.gene058381 | MS.gene60841 | 0.809374 | 1.95E-50 | -1.69E-46 |
| MS.gene058381 | MS.gene60846 | 0.807335 | 5.31E-50 | -1.69E-46 |
| MS.gene058381 | MS.gene60847 | 0.814917 | 1.21E-51 | -1.69E-46 |
| MS.gene058381 | MS.gene61233 | 0.806981 | 6.32E-50 | -1.69E-46 |
| MS.gene058381 | MS.gene65144 | 0.803708 | 3.06E-49 | -1.69E-46 |
| MS.gene058381 | MS.gene71342 | 0.819162 | 1.34E-52 | -1.69E-46 |
| MS.gene058381 | MS.gene71346 | 0.819949 | 8.88E-53 | -1.69E-46 |
| MS.gene058381 | MS.gene72323 | 0.808254 | 3.39E-50 | -1.69E-46 |
| MS.gene058381 | MS.gene72929 | 0.824984 | 6.00E-54 | -1.69E-46 |
| MS.gene058381 | MS.gene74351 | 0.952478 | 2.22E-110 | -1.69E-46 |
| MS.gene058381 | MS.gene75472 | 0.814106 | 1.82E-51 | -1.69E-46 |
| MS.gene058381 | MS.gene76662 | 0.806875 | 6.65E-50 | -1.69E-46 |
| MS.gene058381 | MS.gene77767 | 0.982989 | 1.56E-156 | -1.69E-46 |
| MS.gene058381 | MS.gene78818 | 0.801015 | 1.10E-48 | -1.69E-46 |
| MS.gene058381 | MS.gene79517 | 0.808394 | 3.16E-50 | -1.69E-46 |
| MS.gene058381 | MS.gene80293 | 0.838611 | 2.59E-57 | -1.69E-46 |
| MS.gene058381 | MS.gene80468 | 0.839471 | 1.55E-57 | -1.69E-46 |
| MS.gene058381 | MS.gene80744 | 0.837432 | 5.22E-57 | -1.69E-46 |
| MS.gene058381 | MS.gene81503 | 0.820579 | 6.37E-53 | -1.69E-46 |
| MS.gene058381 | MS.gene81504 | 0.840109 | 1.06E-57 | -1.69E-46 |
| MS.gene058381 | MS.gene81505 | 0.843086 | 1.74E-58 | -1.69E-46 |
| MS.gene058381 | MS.gene81506 | 0.850513 | 1.62E-60 | -1.69E-46 |
| MS.gene058381 | MS.gene81774 | 0.809247 | 2.08E-50 | -1.69E-46 |
| MS.gene058381 | MS.gene83225 | 0.806557 | 7.76E-50 | -1.69E-46 |
| MS.gene058381 | MS.gene83490 | 0.800041 | 1.74E-48 | -1.69E-46 |
| MS.gene058381 | MS.gene84143 | 0.820612 | 6.26E-53 | -1.69E-46 |
| MS.gene058381 | MS.gene85750 | 0.804902 | 1.73E-49 | -1.69E-46 |
| MS.gene058381 | MS.gene90521 | 0.80913 | 2.20E-50 | -1.69E-46 |
| MS.gene058381 | MS.gene90987 | 0.812008 | 5.26E-51 | -1.69E-46 |
| MS.gene058381 | MS.gene91222 | 0.802066 | 6.69E-49 | -1.69E-46 |
| MS.gene058381 | MS.gene91754 | 0.809858 | 1.54E-50 | -1.69E-46 |
| MS.gene058381 | MS.gene94593 | 0.819031 | 1.44E-52 | -1.69E-46 |
| MS.gene058381 | MS.gene98480 | 0.803892 | 2.81E-49 | -1.69E-46 |
| MS.gene058381 | MS.gene98920 | 0.819218 | 1.30E-52 | -1.69E-46 |
| MS.gene058381 | MS.gene99188 | 0.809979 | 1.45E-50 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene058381.t1 | MTR_8g045555 | 92.105 | 152 | 12 | 0 | 1 | 152 | 1 | 152 | 1.47e-103 | 293 |
| MS.gene058381.t1 | MTR_8g045520 | 72.848 | 151 | 41 | 0 | 1 | 151 | 1 | 151 | 9.60e-85 | 245 |
| MS.gene058381.t1 | MTR_8g045570 | 71.053 | 152 | 44 | 0 | 1 | 152 | 1 | 152 | 8.48e-82 | 238 |
| MS.gene058381.t1 | MTR_8g045490 | 71.711 | 152 | 43 | 0 | 1 | 152 | 1 | 152 | 2.25e-81 | 237 |
| MS.gene058381.t1 | MTR_8g045400 | 69.737 | 152 | 45 | 1 | 1 | 152 | 1 | 151 | 3.13e-78 | 229 |
| MS.gene058381.t1 | MTR_8g045695 | 67.763 | 152 | 48 | 1 | 1 | 152 | 1 | 151 | 6.66e-77 | 226 |
| MS.gene058381.t1 | MTR_8g045300 | 65.563 | 151 | 52 | 0 | 1 | 151 | 1 | 151 | 1.67e-76 | 224 |
| MS.gene058381.t1 | MTR_8g045735 | 65.132 | 152 | 52 | 1 | 1 | 152 | 1 | 151 | 1.34e-73 | 217 |
| MS.gene058381.t1 | MTR_8g045665 | 65.132 | 152 | 52 | 1 | 1 | 152 | 1 | 151 | 3.91e-73 | 216 |
| MS.gene058381.t1 | MTR_8g045640 | 65.132 | 152 | 52 | 1 | 1 | 152 | 1 | 151 | 2.14e-72 | 214 |
| MS.gene058381.t1 | MTR_8g045560 | 63.816 | 152 | 54 | 1 | 1 | 152 | 1 | 151 | 2.40e-70 | 209 |
| MS.gene058381.t1 | MTR_1g030810 | 43.137 | 153 | 84 | 2 | 1 | 151 | 1 | 152 | 2.66e-41 | 135 |
| MS.gene058381.t1 | MTR_1g030820 | 37.255 | 153 | 93 | 2 | 1 | 151 | 1 | 152 | 1.11e-37 | 126 |
| MS.gene058381.t1 | MTR_1g030840 | 39.103 | 156 | 87 | 4 | 1 | 149 | 1 | 155 | 1.29e-32 | 114 |
| MS.gene058381.t1 | MTR_1g031640 | 35.811 | 148 | 86 | 4 | 7 | 152 | 7 | 147 | 1.02e-27 | 100 |
| MS.gene058381.t1 | MTR_8g012550 | 33.103 | 145 | 94 | 3 | 6 | 149 | 8 | 150 | 3.49e-26 | 97.1 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene058381.t1 | AT1G70890 | 34.000 | 150 | 96 | 3 | 3 | 150 | 7 | 155 | 3.07e-27 | 100 |
| MS.gene058381.t1 | AT5G28010 | 34.000 | 150 | 96 | 3 | 3 | 150 | 15 | 163 | 1.20e-26 | 98.6 |
| MS.gene058381.t1 | AT5G28010 | 34.000 | 150 | 96 | 3 | 3 | 150 | 15 | 163 | 1.20e-26 | 98.6 |
| MS.gene058381.t1 | AT1G70840 | 32.667 | 150 | 98 | 3 | 3 | 150 | 20 | 168 | 1.46e-26 | 98.6 |
| MS.gene058381.t1 | AT1G70830 | 34.667 | 150 | 95 | 3 | 3 | 150 | 22 | 170 | 2.01e-26 | 98.2 |
| MS.gene058381.t1 | AT1G70830 | 34.667 | 150 | 95 | 3 | 3 | 150 | 22 | 170 | 3.46e-26 | 98.6 |
| MS.gene058381.t1 | AT1G70830 | 34.667 | 150 | 95 | 3 | 3 | 150 | 22 | 170 | 1.28e-25 | 98.2 |
| MS.gene058381.t1 | AT1G70830 | 34.667 | 150 | 95 | 3 | 3 | 150 | 22 | 170 | 4.19e-25 | 98.6 |
| MS.gene058381.t1 | AT1G70830 | 33.333 | 150 | 97 | 3 | 3 | 150 | 184 | 332 | 2.10e-23 | 94.0 |
| MS.gene058381.t1 | AT1G70850 | 32.667 | 150 | 98 | 3 | 3 | 150 | 9 | 157 | 4.39e-24 | 94.0 |
| MS.gene058381.t1 | AT1G70830 | 33.333 | 150 | 97 | 3 | 3 | 150 | 137 | 285 | 8.32e-24 | 94.4 |
| MS.gene058381.t1 | AT1G70830 | 32.258 | 93 | 62 | 1 | 59 | 150 | 31 | 123 | 5.24e-14 | 67.8 |
| MS.gene058381.t1 | AT1G70850 | 32.667 | 150 | 98 | 3 | 3 | 150 | 9 | 157 | 1.43e-23 | 94.0 |
| MS.gene058381.t1 | AT1G70850 | 33.571 | 140 | 90 | 3 | 13 | 150 | 175 | 313 | 4.43e-23 | 92.8 |
| MS.gene058381.t1 | AT1G70850 | 32.667 | 150 | 98 | 3 | 3 | 150 | 9 | 157 | 1.43e-23 | 94.0 |
| MS.gene058381.t1 | AT1G70850 | 33.571 | 140 | 90 | 3 | 13 | 150 | 175 | 313 | 4.43e-23 | 92.8 |
| MS.gene058381.t1 | AT1G23130 | 30.201 | 149 | 102 | 2 | 3 | 150 | 10 | 157 | 3.94e-23 | 89.4 |
| MS.gene058381.t1 | AT1G70870 | 35.099 | 151 | 85 | 4 | 1 | 151 | 1 | 138 | 1.37e-22 | 87.4 |
| MS.gene058381.t1 | AT5G28000 | 28.000 | 150 | 105 | 3 | 3 | 150 | 13 | 161 | 1.54e-20 | 82.8 |
| MS.gene058381.t1 | AT1G23120 | 27.083 | 144 | 100 | 3 | 7 | 150 | 7 | 145 | 2.89e-20 | 81.6 |
| MS.gene058381.t1 | AT1G70880 | 28.000 | 150 | 105 | 3 | 3 | 150 | 8 | 156 | 3.56e-20 | 81.6 |
| MS.gene058381.t1 | AT1G23120 | 27.083 | 144 | 100 | 3 | 7 | 150 | 57 | 195 | 1.12e-19 | 81.6 |
| MS.gene058381.t1 | AT1G14930 | 30.968 | 155 | 100 | 4 | 1 | 152 | 1 | 151 | 2.76e-19 | 79.3 |
| MS.gene058381.t1 | AT4G23670 | 30.968 | 155 | 100 | 4 | 1 | 152 | 1 | 151 | 7.05e-17 | 73.2 |
| MS.gene058381.t1 | AT2G01520 | 30.323 | 155 | 101 | 4 | 1 | 152 | 1 | 151 | 7.68e-17 | 72.8 |
| MS.gene058381.t1 | AT1G24020 | 30.065 | 153 | 103 | 3 | 1 | 151 | 1 | 151 | 1.14e-15 | 70.1 |
| MS.gene058381.t1 | AT1G24020 | 30.065 | 153 | 103 | 3 | 1 | 151 | 1 | 151 | 1.14e-15 | 70.1 |
| MS.gene058381.t1 | AT4G14060 | 30.323 | 155 | 101 | 4 | 1 | 152 | 1 | 151 | 3.70e-15 | 68.6 |
| MS.gene058381.t1 | AT1G35260 | 22.517 | 151 | 113 | 3 | 2 | 150 | 1 | 149 | 4.52e-15 | 68.6 |
| MS.gene058381.t1 | AT1G14940 | 28.477 | 151 | 105 | 3 | 1 | 150 | 1 | 149 | 6.91e-15 | 67.8 |
| MS.gene058381.t1 | AT4G23680 | 29.032 | 155 | 103 | 4 | 1 | 152 | 1 | 151 | 1.48e-14 | 67.0 |
| MS.gene058381.t1 | AT2G01530 | 29.032 | 155 | 103 | 4 | 1 | 152 | 1 | 151 | 1.69e-14 | 67.0 |
| MS.gene058381.t1 | AT1G14950 | 28.758 | 153 | 104 | 4 | 1 | 151 | 1 | 150 | 1.80e-13 | 64.3 |
| MS.gene058381.t1 | AT1G30990 | 25.828 | 151 | 108 | 4 | 1 | 150 | 1 | 148 | 1.18e-11 | 59.3 |
| MS.gene058381.t1 | AT1G14960 | 26.316 | 152 | 109 | 3 | 1 | 151 | 1 | 150 | 8.49e-11 | 57.0 |
| MS.gene058381.t1 | AT3G26450 | 26.282 | 156 | 107 | 5 | 1 | 152 | 1 | 152 | 8.79e-11 | 57.0 |
Find 29 sgRNAs with CRISPR-Local
Find 86 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| AAGCTCTTCGGTGGAGATAT+TGG | 0.245046 | 3.2:+2634723 | MS.gene058381:CDS |
| AGCTCTTCGGTGGAGATATT+GGG | 0.329670 | 3.2:+2634724 | MS.gene058381:CDS |
| ATATTCAATTGTCCATTTGA+TGG | 0.352752 | 3.2:-2634808 | None:intergenic |
| GTCATCCCCTTGATGTAGCT+TGG | 0.401893 | 3.2:-2633212 | None:intergenic |
| AAACATTGGACTTATGTCAT+AGG | 0.403651 | 3.2:+2633256 | MS.gene058381:CDS |
| TAGAGATATTGATGCTCATC+TGG | 0.421031 | 3.2:+2634893 | MS.gene058381:CDS |
| ATTCAAATAATTGATAATAG+TGG | 0.432059 | 3.2:+2634774 | MS.gene058381:CDS |
| ACGTAGTCCATCCAGCCATT+AGG | 0.444330 | 3.2:-2634858 | None:intergenic |
| AGATATTGGGGAGAACTATA+AGG | 0.448937 | 3.2:+2634737 | MS.gene058381:CDS |
| AGCTACATCAAGGGGATGAC+TGG | 0.453220 | 3.2:+2633215 | MS.gene058381:CDS |
| GAGGATATTGACCCTCCTAA+TGG | 0.460289 | 3.2:+2634843 | MS.gene058381:CDS |
| GCAGCAGTGCTGCCATCAAA+TGG | 0.475803 | 3.2:+2634796 | MS.gene058381:CDS |
| GGTAAACTTAGTACTGAACT+TGG | 0.476618 | 3.2:+2633103 | MS.gene058381:CDS |
| CTTCCAAGGATGGTGCTCAA+TGG | 0.508173 | 3.2:+2633082 | None:intergenic |
| AAACTCACTTACAAGCTCTT+CGG | 0.512951 | 3.2:+2634711 | MS.gene058381:CDS |
| CATGAAACCAAGCTACATCA+AGG | 0.525025 | 3.2:+2633205 | MS.gene058381:CDS |
| ATATTGACCCTCCTAATGGC+TGG | 0.525673 | 3.2:+2634847 | MS.gene058381:CDS |
| TTTCATGAACTCTTTCACAA+TGG | 0.533960 | 3.2:-2633189 | None:intergenic |
| TAGTCCATCCAGCCATTAGG+AGG | 0.553714 | 3.2:-2634855 | None:intergenic |
| TGACCCTCCTAATGGCTGGA+TGG | 0.577019 | 3.2:+2634851 | MS.gene058381:CDS |
| TGACTGGCATCACACTGATA+CGG | 0.601188 | 3.2:+2633231 | MS.gene058381:CDS |
| GTAAACTTAGTACTGAACTT+GGG | 0.605653 | 3.2:+2633104 | MS.gene058381:CDS |
| ACACTGATACGGTTAAACAT+TGG | 0.605669 | 3.2:+2633242 | MS.gene058381:CDS |
| GCTCTTCGGTGGAGATATTG+GGG | 0.615379 | 3.2:+2634725 | MS.gene058381:CDS |
| CTCACTTACAAGCTCTTCGG+TGG | 0.619750 | 3.2:+2634714 | MS.gene058381:CDS |
| ATGAAACCAAGCTACATCAA+GGG | 0.623559 | 3.2:+2633206 | MS.gene058381:CDS |
| TGAAACCAAGCTACATCAAG+GGG | 0.635241 | 3.2:+2633207 | MS.gene058381:CDS |
| AGTCCATCCAGCCATTAGGA+GGG | 0.659406 | 3.2:-2634854 | None:intergenic |
| TGAATATGAGAAGATCAATG+AGG | 0.677949 | 3.2:+2634824 | MS.gene058381:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | AATTTAAGAACAATATTTGT+AGG | - | chr3.2:2634528-2634547 | None:intergenic | 15.0% |
| !! | ACGTAAATTGTTAAAATAAA+AGG | - | chr3.2:2633611-2633630 | None:intergenic | 15.0% |
| !!! | ATTCAAATAATTGATAATAG+TGG | + | chr3.2:2634774-2634793 | MS.gene058381:CDS | 15.0% |
| !!! | CTTTCTAATTTTATCATTTT+GGG | - | chr3.2:2633537-2633556 | None:intergenic | 15.0% |
| !!! | TAAAATTACTTTAAAACAAC+TGG | + | chr3.2:2634442-2634461 | MS.gene058381:intron | 15.0% |
| !! | ATTATTGAAAACGAAAGATA+AGG | - | chr3.2:2634576-2634595 | None:intergenic | 20.0% |
| !! | CATATGAAAATAAAATTGTG+TGG | - | chr3.2:2634608-2634627 | None:intergenic | 20.0% |
| !! | TATTCTAATTAAGGTCTTTA+AGG | + | chr3.2:2633483-2633502 | MS.gene058381:intron | 20.0% |
| !!! | CACACAATTTTATTTTCATA+TGG | + | chr3.2:2634606-2634625 | MS.gene058381:intron | 20.0% |
| !!! | GCTTTCTAATTTTATCATTT+TGG | - | chr3.2:2633538-2633557 | None:intergenic | 20.0% |
| !!! | TAATTTTATCATTTTGGGAT+AGG | - | chr3.2:2633532-2633551 | None:intergenic | 20.0% |
| ! | ATATTCAATTGTCCATTTGA+TGG | - | chr3.2:2634811-2634830 | None:intergenic | 25.0% |
| ! | CTTAAACTAGAAATCTGAAA+AGG | + | chr3.2:2634485-2634504 | MS.gene058381:intron | 25.0% |
| !! | TACATGTACTATTTTATCGT+TGG | - | chr3.2:2634090-2634109 | None:intergenic | 25.0% |
| AAAACAACTGGTACAAAACT+TGG | + | chr3.2:2634454-2634473 | MS.gene058381:intron | 30.0% | |
| AAACATTGGACTTATGTCAT+AGG | + | chr3.2:2633256-2633275 | MS.gene058381:CDS | 30.0% | |
| AAACTTAGAGATCCAACTAA+GGG | - | chr3.2:2634259-2634278 | None:intergenic | 30.0% | |
| ACAAGTTAACATTTCCCTTA+GGG | + | chr3.2:2633806-2633825 | MS.gene058381:intron | 30.0% | |
| GTGAATAAGATTTGCAGAAT+CGG | + | chr3.2:2634405-2634424 | MS.gene058381:intron | 30.0% | |
| TAAACTTAGAGATCCAACTA+AGG | - | chr3.2:2634260-2634279 | None:intergenic | 30.0% | |
| TCTTAAGTGTTACACTTTGT+GGG | - | chr3.2:2634025-2634044 | None:intergenic | 30.0% | |
| TGAATATGAGAAGATCAATG+AGG | + | chr3.2:2634824-2634843 | MS.gene058381:CDS | 30.0% | |
| TTTCATGAACTCTTTCACAA+TGG | - | chr3.2:2633192-2633211 | None:intergenic | 30.0% | |
| ! | GATGAATTGATGATACTATG+AGG | - | chr3.2:2633945-2633964 | None:intergenic | 30.0% |
| ! | GTAAACTTAGTACTGAACTT+GGG | + | chr3.2:2633104-2633123 | MS.gene058381:CDS | 30.0% |
| ! | TTGATAGTAAGTAAACGTAG+AGG | - | chr3.2:2633582-2633601 | None:intergenic | 30.0% |
| !! | AGTGTTAACTTGTGTCTTTA+GGG | + | chr3.2:2633644-2633663 | MS.gene058381:intron | 30.0% |
| !!! | AGTCATGAGAATGACTTTTT+TGG | + | chr3.2:2633844-2633863 | MS.gene058381:intron | 30.0% |
| !!! | GATTCTAGTGTGTTTTTTGA+AGG | + | chr3.2:2634376-2634395 | MS.gene058381:intron | 30.0% |
| AAACTCACTTACAAGCTCTT+CGG | + | chr3.2:2634711-2634730 | MS.gene058381:CDS | 35.0% | |
| AATCATTACCTTGTGTCTTG+AGG | + | chr3.2:2633781-2633800 | MS.gene058381:intron | 35.0% | |
| ACACTGATACGGTTAAACAT+TGG | + | chr3.2:2633242-2633261 | MS.gene058381:CDS | 35.0% | |
| AGATATTGGGGAGAACTATA+AGG | + | chr3.2:2634737-2634756 | MS.gene058381:CDS | 35.0% | |
| AGTAATGCTTTGCAACATCT+TGG | - | chr3.2:2634116-2634135 | None:intergenic | 35.0% | |
| ATCATTACCTTGTGTCTTGA+GGG | + | chr3.2:2633782-2633801 | MS.gene058381:intron | 35.0% | |
| ATGAAACCAAGCTACATCAA+GGG | + | chr3.2:2633206-2633225 | MS.gene058381:CDS | 35.0% | |
| ATGCCATTGTACAAATAGTG+GGG | - | chr3.2:2633910-2633929 | None:intergenic | 35.0% | |
| CTTAAGTGTTACACTTTGTG+GGG | - | chr3.2:2634024-2634043 | None:intergenic | 35.0% | |
| GACAAGTTAACATTTCCCTT+AGG | + | chr3.2:2633805-2633824 | MS.gene058381:intron | 35.0% | |
| GATGCCATTGTACAAATAGT+GGG | - | chr3.2:2633911-2633930 | None:intergenic | 35.0% | |
| GTACAATGGCATCAAAAGTT+TGG | + | chr3.2:2633918-2633937 | MS.gene058381:intron | 35.0% | |
| GTCTTAAGTGTTACACTTTG+TGG | - | chr3.2:2634026-2634045 | None:intergenic | 35.0% | |
| TAGAGATATTGATGCTCATC+TGG | + | chr3.2:2634893-2634912 | MS.gene058381:CDS | 35.0% | |
| TGATGCCATTGTACAAATAG+TGG | - | chr3.2:2633912-2633931 | None:intergenic | 35.0% | |
| TGTTACGCTTATAACAAGGT+TGG | - | chr3.2:2634001-2634020 | None:intergenic | 35.0% | |
| TTCCCTTAGGGAAAATTCTA+TGG | + | chr3.2:2633818-2633837 | MS.gene058381:intron | 35.0% | |
| ! | CACCATAGAATTTTCCCTAA+GGG | - | chr3.2:2633823-2633842 | None:intergenic | 35.0% |
| ! | GCTTTTACATGTTCTCTTGT+TGG | + | chr3.2:2633556-2633575 | MS.gene058381:intron | 35.0% |
| ! | GGTAAACTTAGTACTGAACT+TGG | + | chr3.2:2633103-2633122 | MS.gene058381:CDS | 35.0% |
| ! | TCACCATAGAATTTTCCCTA+AGG | - | chr3.2:2633824-2633843 | None:intergenic | 35.0% |
| !! | CAGTGTTAACTTGTGTCTTT+AGG | + | chr3.2:2633643-2633662 | MS.gene058381:intron | 35.0% |
| !!! | GTGTGTTTTTTGAAGGACTA+AGG | + | chr3.2:2634383-2634402 | MS.gene058381:intron | 35.0% |
| ACACCCCACTATTTGTACAA+TGG | + | chr3.2:2633904-2633923 | MS.gene058381:intron | 40.0% | |
| CATGAAACCAAGCTACATCA+AGG | + | chr3.2:2633205-2633224 | MS.gene058381:CDS | 40.0% | |
| TAGAGATCCAACTAAGGGAA+GGG | - | chr3.2:2634254-2634273 | None:intergenic | 40.0% | |
| TCATTACCTTGTGTCTTGAG+GGG | + | chr3.2:2633783-2633802 | MS.gene058381:intron | 40.0% | |
| TGAAACCAAGCTACATCAAG+GGG | + | chr3.2:2633207-2633226 | MS.gene058381:CDS | 40.0% | |
| TTAGAGATCCAACTAAGGGA+AGG | - | chr3.2:2634255-2634274 | None:intergenic | 40.0% | |
| !! | GTCTTTAAGGAGTACTCAAG+AGG | + | chr3.2:2633496-2633515 | MS.gene058381:intron | 40.0% |
| AAGCTCTTCGGTGGAGATAT+TGG | + | chr3.2:2634723-2634742 | MS.gene058381:CDS | 45.0% | |
| AGCTCTTCGGTGGAGATATT+GGG | + | chr3.2:2634724-2634743 | MS.gene058381:CDS | 45.0% | |
| ATATTGACCCTCCTAATGGC+TGG | + | chr3.2:2634847-2634866 | MS.gene058381:CDS | 45.0% | |
| GAGGATATTGACCCTCCTAA+TGG | + | chr3.2:2634843-2634862 | MS.gene058381:CDS | 45.0% | |
| TGACTGGCATCACACTGATA+CGG | + | chr3.2:2633231-2633250 | MS.gene058381:CDS | 45.0% | |
| TGCCATCTGTTGTCATGTGA+CGG | - | chr3.2:2634641-2634660 | None:intergenic | 45.0% | |
| ! | CTACTAGTTTCTCCTCCTGA+AGG | + | chr3.2:2634281-2634300 | MS.gene058381:intron | 45.0% |
| ! | GGGGTGTTACGCTTATAACA+AGG | - | chr3.2:2634005-2634024 | None:intergenic | 45.0% |
| !! | TATAAAAAATATTCTAATTA+AGG | + | chr3.2:2633474-2633493 | MS.gene058381:intron | 5.0% |
| !!! | AATTGAATTTAAAAATTTTT+AGG | - | chr3.2:2633360-2633379 | None:intergenic | 5.0% |
| ACGTAGTCCATCCAGCCATT+AGG | - | chr3.2:2634861-2634880 | None:intergenic | 50.0% | |
| ACTTGTCCCCTCAAGACACA+AGG | - | chr3.2:2633792-2633811 | None:intergenic | 50.0% | |
| AGAAGCTCCCTTCCCTTAGT+TGG | + | chr3.2:2634244-2634263 | MS.gene058381:intron | 50.0% | |
| AGCTACATCAAGGGGATGAC+TGG | + | chr3.2:2633215-2633234 | MS.gene058381:CDS | 50.0% | |
| AGTCCATCCAGCCATTAGGA+GGG | - | chr3.2:2634857-2634876 | None:intergenic | 50.0% | |
| CTCACTTACAAGCTCTTCGG+TGG | + | chr3.2:2634714-2634733 | MS.gene058381:CDS | 50.0% | |
| GAATCGAACCTGAGACCTTC+AGG | - | chr3.2:2634299-2634318 | None:intergenic | 50.0% | |
| GCCATCTGTTGTCATGTGAC+GGG | - | chr3.2:2634640-2634659 | None:intergenic | 50.0% | |
| GCTCTTCGGTGGAGATATTG+GGG | + | chr3.2:2634725-2634744 | MS.gene058381:CDS | 50.0% | |
| GTCATCCCCTTGATGTAGCT+TGG | - | chr3.2:2633215-2633234 | None:intergenic | 50.0% | |
| TAGTCCATCCAGCCATTAGG+AGG | - | chr3.2:2634858-2634877 | None:intergenic | 50.0% | |
| TCCCGTCACATGACAACAGA+TGG | + | chr3.2:2634636-2634655 | MS.gene058381:intron | 50.0% | |
| TTTCTCCTCCTGAAGGTCTC+AGG | + | chr3.2:2634288-2634307 | MS.gene058381:intron | 50.0% | |
| GCAGCAGTGCTGCCATCAAA+TGG | + | chr3.2:2634796-2634815 | MS.gene058381:CDS | 55.0% | |
| GGAGCTTCTACAGTGCAGTC+AGG | - | chr3.2:2634233-2634252 | None:intergenic | 55.0% | |
| TCGAACCTGAGACCTTCAGG+AGG | - | chr3.2:2634296-2634315 | None:intergenic | 55.0% | |
| TGACCCTCCTAATGGCTGGA+TGG | + | chr3.2:2634851-2634870 | MS.gene058381:CDS | 55.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr3.2 | gene | 2633091 | 2634926 | 2633091 | ID=MS.gene058381 |
| chr3.2 | mRNA | 2633091 | 2634926 | 2633091 | ID=MS.gene058381.t1;Parent=MS.gene058381 |
| chr3.2 | exon | 2633091 | 2633277 | 2633091 | ID=MS.gene058381.t1.exon1;Parent=MS.gene058381.t1 |
| chr3.2 | CDS | 2633091 | 2633277 | 2633091 | ID=cds.MS.gene058381.t1;Parent=MS.gene058381.t1 |
| chr3.2 | exon | 2634655 | 2634926 | 2634655 | ID=MS.gene058381.t1.exon2;Parent=MS.gene058381.t1 |
| chr3.2 | CDS | 2634655 | 2634926 | 2634655 | ID=cds.MS.gene058381.t1;Parent=MS.gene058381.t1 |
| Gene Sequence |
| Protein sequence |
