Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene83507.t1 | KEH39684.1 | 86.5 | 89 | 12 | 0 | 1 | 89 | 172 | 260 | 5.90E-33 | 149.8 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene83507.t1 | Q9LU41 | 66.3 | 89 | 30 | 0 | 1 | 89 | 998 | 1086 | 8.6e-25 | 114.0 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene83507.t1 | A0A072VCB6 | 86.5 | 89 | 12 | 0 | 1 | 89 | 172 | 260 | 4.2e-33 | 149.8 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene050670 | MS.gene83507 | 0.802121 | 6.52E-49 | -1.69E-46 |
| MS.gene052134 | MS.gene83507 | 0.815445 | 9.21E-52 | -1.69E-46 |
| MS.gene052135 | MS.gene83507 | 0.821805 | 3.32E-53 | -1.69E-46 |
| MS.gene052505 | MS.gene83507 | 0.816881 | 4.40E-52 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene83507.t1 | MTR_2g105690 | 86.517 | 89 | 12 | 0 | 1 | 89 | 172 | 260 | 1.68e-48 | 154 |
| MS.gene83507.t1 | MTR_4g008170 | 62.821 | 78 | 29 | 0 | 1 | 78 | 1015 | 1092 | 1.46e-28 | 107 |
| MS.gene83507.t1 | MTR_4g008170 | 62.821 | 78 | 29 | 0 | 1 | 78 | 1022 | 1099 | 1.46e-28 | 107 |
| MS.gene83507.t1 | MTR_2g038310 | 58.140 | 86 | 32 | 1 | 2 | 87 | 1004 | 1085 | 4.70e-27 | 103 |
| MS.gene83507.t1 | MTR_6g016470 | 50.704 | 71 | 35 | 0 | 2 | 72 | 941 | 1011 | 7.33e-20 | 82.4 |
| MS.gene83507.t1 | MTR_8g013780 | 49.296 | 71 | 36 | 0 | 2 | 72 | 493 | 563 | 2.35e-19 | 81.3 |
| MS.gene83507.t1 | MTR_7g095710 | 52.174 | 69 | 33 | 0 | 3 | 71 | 975 | 1043 | 9.41e-19 | 79.3 |
| MS.gene83507.t1 | MTR_8g090125 | 44.928 | 69 | 38 | 0 | 1 | 69 | 903 | 971 | 8.34e-12 | 59.7 |
| MS.gene83507.t1 | MTR_4g008650 | 41.333 | 75 | 44 | 0 | 1 | 75 | 945 | 1019 | 1.53e-11 | 58.9 |
| MS.gene83507.t1 | MTR_4g096990 | 43.284 | 67 | 38 | 0 | 1 | 67 | 946 | 1012 | 4.38e-11 | 57.8 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene83507.t1 | AT3G21180 | 66.292 | 89 | 30 | 0 | 1 | 89 | 998 | 1086 | 1.52e-32 | 118 |
| MS.gene83507.t1 | AT5G57110 | 56.000 | 75 | 33 | 0 | 2 | 76 | 982 | 1056 | 1.09e-25 | 99.4 |
| MS.gene83507.t1 | AT5G57110 | 56.000 | 75 | 33 | 0 | 2 | 76 | 982 | 1056 | 1.09e-25 | 99.4 |
| MS.gene83507.t1 | AT5G57110 | 56.000 | 75 | 33 | 0 | 2 | 76 | 982 | 1056 | 1.09e-25 | 99.4 |
| MS.gene83507.t1 | AT4G29900 | 56.757 | 74 | 32 | 0 | 1 | 74 | 983 | 1056 | 1.57e-25 | 98.6 |
| MS.gene83507.t1 | AT4G29900 | 56.757 | 74 | 32 | 0 | 1 | 74 | 983 | 1056 | 1.57e-25 | 98.6 |
| MS.gene83507.t1 | AT3G63380 | 48.571 | 70 | 36 | 0 | 2 | 71 | 941 | 1010 | 3.89e-16 | 72.0 |
| MS.gene83507.t1 | AT3G57330 | 50.746 | 67 | 33 | 0 | 1 | 67 | 939 | 1005 | 6.65e-12 | 60.1 |
| MS.gene83507.t1 | AT3G57330 | 50.746 | 67 | 33 | 0 | 1 | 67 | 884 | 950 | 7.04e-12 | 60.1 |
| MS.gene83507.t1 | AT2G41560 | 49.254 | 67 | 34 | 0 | 1 | 67 | 942 | 1008 | 1.34e-11 | 58.9 |
| MS.gene83507.t1 | AT2G41560 | 49.254 | 67 | 34 | 0 | 1 | 67 | 840 | 906 | 1.47e-11 | 58.9 |
| MS.gene83507.t1 | AT2G41560 | 49.254 | 67 | 34 | 0 | 1 | 67 | 840 | 906 | 1.47e-11 | 58.9 |
| MS.gene83507.t1 | AT2G41560 | 49.254 | 67 | 34 | 0 | 1 | 67 | 840 | 906 | 1.47e-11 | 58.9 |
Find 26 sgRNAs with CRISPR-Local
Find 39 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| AGCTGGCCACTTGCGGTTAT+TGG | 0.177695 | 2.4:+283809 | MS.gene83507:intron |
| GGCTTGCTTGTATTGGTATT+GGG | 0.243098 | 2.4:+283641 | MS.gene83507:CDS |
| TGGCTTGCTTGTATTGGTAT+TGG | 0.266103 | 2.4:+283640 | MS.gene83507:CDS |
| GGACCGTGACATTGGTGTTT+TGG | 0.311444 | 2.4:-283849 | None:intergenic |
| CAACAACAGTGAGGCTGAAT+TGG | 0.325866 | 2.4:+283611 | MS.gene83507:CDS |
| GACCATCTCTTCGTCGGAAT+TGG | 0.347372 | 2.4:+283448 | MS.gene83507:CDS |
| CAGCAGTGGCTTGCTTGTAT+TGG | 0.367797 | 2.4:+283634 | MS.gene83507:CDS |
| GTATTGGTATTGGGCTGGTC+AGG | 0.406693 | 2.4:+283650 | MS.gene83507:CDS |
| TGCTTGTATTGGTATTGGGC+TGG | 0.426575 | 2.4:+283645 | MS.gene83507:CDS |
| GGTCCATCTTGAGGGTGTTT+AGG | 0.459145 | 2.4:+283867 | MS.gene83507:CDS |
| CATCTCTTCGTCGGAATTGG+TGG | 0.473960 | 2.4:+283451 | MS.gene83507:CDS |
| ATAATCATCATCGAGTTCCT+TGG | 0.489778 | 2.4:+283580 | MS.gene83507:CDS |
| TAATCATCATCGAGTTCCTT+GGG | 0.490653 | 2.4:+283581 | MS.gene83507:CDS |
| AATGTCACGGTCCATCTTGA+GGG | 0.515908 | 2.4:+283859 | MS.gene83507:CDS |
| TTCCAACAGCTGGCCACTTG+CGG | 0.529368 | 2.4:+283802 | MS.gene83507:intron |
| CAATGTCACGGTCCATCTTG+AGG | 0.539876 | 2.4:+283858 | MS.gene83507:CDS |
| CTCAAGATGGACCGTGACAT+TGG | 0.543571 | 2.4:-283857 | None:intergenic |
| CTGTTGTTGCAAACTTCCCA+AGG | 0.548024 | 2.4:-283597 | None:intergenic |
| CACCAATTCCGACGAAGAGA+TGG | 0.548137 | 2.4:-283450 | None:intergenic |
| ATCTCTTCGTCGGAATTGGT+GGG | 0.549056 | 2.4:+283452 | MS.gene83507:CDS |
| GCGCCTAAACACCCTCAAGA+TGG | 0.566557 | 2.4:-283870 | None:intergenic |
| TGAGGCTGAATTGGCAGCAG+TGG | 0.571899 | 2.4:+283620 | MS.gene83507:CDS |
| GACTCAGACCATCTCTTCGT+CGG | 0.581982 | 2.4:+283442 | MS.gene83507:CDS |
| GAAGGGATGAATGTCTTCAA+AGG | 0.598151 | 2.4:+283416 | None:intergenic |
| TTCCCAAAACACCAATGTCA+CGG | 0.635732 | 2.4:+283846 | MS.gene83507:CDS |
| GGAAGTTTGCAACAACAGTG+AGG | 0.695622 | 2.4:+283602 | MS.gene83507:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | TTGTAAAATGATTATCAATA+TGG | - | chr2.4:283707-283726 | None:intergenic | 15.0% |
| ! | CTTCATATGATATTACTAAC+CGG | + | chr2.4:283768-283787 | MS.gene83507:intron | 25.0% |
| GAAGCTAAGATATATTGAGA+GGG | - | chr2.4:283752-283771 | None:intergenic | 30.0% | |
| TGAAGCTAAGATATATTGAG+AGG | - | chr2.4:283753-283772 | None:intergenic | 30.0% | |
| ! | CTTTTGTACTTCAGGTAAAT+AGG | + | chr2.4:283480-283499 | MS.gene83507:intron | 30.0% |
| ATAATCATCATCGAGTTCCT+TGG | + | chr2.4:283580-283599 | MS.gene83507:CDS | 35.0% | |
| TAATCATCATCGAGTTCCTT+GGG | + | chr2.4:283581-283600 | MS.gene83507:CDS | 35.0% | |
| TTAGTACACTCAACTGTAGA+AGG | + | chr2.4:283673-283692 | MS.gene83507:intron | 35.0% | |
| ! | AATTTTCCAATAACCGCAAG+TGG | - | chr2.4:283818-283837 | None:intergenic | 35.0% |
| TGTTGGAAGGAAAAATCAGC+CGG | - | chr2.4:283790-283809 | None:intergenic | 40.0% | |
| TTCCCAAAACACCAATGTCA+CGG | + | chr2.4:283846-283865 | MS.gene83507:CDS | 40.0% | |
| ! | GGCTTGCTTGTATTGGTATT+GGG | + | chr2.4:283641-283660 | MS.gene83507:CDS | 40.0% |
| ! | TGATTTTTCCTTCCAACAGC+TGG | + | chr2.4:283792-283811 | MS.gene83507:intron | 40.0% |
| !! | TGGCTTGCTTGTATTGGTAT+TGG | + | chr2.4:283640-283659 | MS.gene83507:CDS | 40.0% |
| AATGTCACGGTCCATCTTGA+GGG | + | chr2.4:283859-283878 | MS.gene83507:CDS | 45.0% | |
| ATCTCTTCGTCGGAATTGGT+GGG | + | chr2.4:283452-283471 | MS.gene83507:CDS | 45.0% | |
| CAACAACAGTGAGGCTGAAT+TGG | + | chr2.4:283611-283630 | MS.gene83507:CDS | 45.0% | |
| CTGTTGTTGCAAACTTCCCA+AGG | - | chr2.4:283600-283619 | None:intergenic | 45.0% | |
| GGAAGTTTGCAACAACAGTG+AGG | + | chr2.4:283602-283621 | MS.gene83507:CDS | 45.0% | |
| ! | GGGAGTGACTTTTGTACTTC+AGG | + | chr2.4:283472-283491 | MS.gene83507:CDS | 45.0% |
| ! | TGCTTGTATTGGTATTGGGC+TGG | + | chr2.4:283645-283664 | MS.gene83507:CDS | 45.0% |
| !! | GACCGTGACATTGGTGTTTT+GGG | - | chr2.4:283851-283870 | None:intergenic | 45.0% |
| !!! | GACATTGGTGTTTTGGGAAC+TGG | - | chr2.4:283845-283864 | None:intergenic | 45.0% |
| CAATGTCACGGTCCATCTTG+AGG | + | chr2.4:283858-283877 | MS.gene83507:CDS | 50.0% | |
| CACCAATTCCGACGAAGAGA+TGG | - | chr2.4:283453-283472 | None:intergenic | 50.0% | |
| CATCTCTTCGTCGGAATTGG+TGG | + | chr2.4:283451-283470 | MS.gene83507:CDS | 50.0% | |
| CTCAAGATGGACCGTGACAT+TGG | - | chr2.4:283860-283879 | None:intergenic | 50.0% | |
| GACCATCTCTTCGTCGGAAT+TGG | + | chr2.4:283448-283467 | MS.gene83507:CDS | 50.0% | |
| GACTCAGACCATCTCTTCGT+CGG | + | chr2.4:283442-283461 | MS.gene83507:CDS | 50.0% | |
| !! | CAGCAGTGGCTTGCTTGTAT+TGG | + | chr2.4:283634-283653 | MS.gene83507:CDS | 50.0% |
| !! | GGACCGTGACATTGGTGTTT+TGG | - | chr2.4:283852-283871 | None:intergenic | 50.0% |
| !! | GGTCCATCTTGAGGGTGTTT+AGG | + | chr2.4:283867-283886 | MS.gene83507:CDS | 50.0% |
| !! | GTATTGGTATTGGGCTGGTC+AGG | + | chr2.4:283650-283669 | MS.gene83507:CDS | 50.0% |
| AGCTGGCCACTTGCGGTTAT+TGG | + | chr2.4:283809-283828 | MS.gene83507:intron | 55.0% | |
| GCGCCTAAACACCCTCAAGA+TGG | - | chr2.4:283873-283892 | None:intergenic | 55.0% | |
| TGAGGCTGAATTGGCAGCAG+TGG | + | chr2.4:283620-283639 | MS.gene83507:CDS | 55.0% | |
| TTCCAACAGCTGGCCACTTG+CGG | + | chr2.4:283802-283821 | MS.gene83507:intron | 55.0% | |
| AACCGCAAGTGGCCAGCTGT+TGG | - | chr2.4:283807-283826 | None:intergenic | 60.0% | |
| GCAAGTGGCCAGCTGTTGGA+AGG | - | chr2.4:283803-283822 | None:intergenic | 60.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr2.4 | gene | 283422 | 283916 | 283422 | ID=MS.gene83507 |
| chr2.4 | mRNA | 283422 | 283916 | 283422 | ID=MS.gene83507.t1;Parent=MS.gene83507 |
| chr2.4 | exon | 283422 | 283493 | 283422 | ID=MS.gene83507.t1.exon1;Parent=MS.gene83507.t1 |
| chr2.4 | CDS | 283422 | 283493 | 283422 | ID=cds.MS.gene83507.t1;Parent=MS.gene83507.t1 |
| chr2.4 | exon | 283580 | 283671 | 283580 | ID=MS.gene83507.t1.exon2;Parent=MS.gene83507.t1 |
| chr2.4 | CDS | 283580 | 283671 | 283580 | ID=cds.MS.gene83507.t1;Parent=MS.gene83507.t1 |
| chr2.4 | exon | 283811 | 283916 | 283811 | ID=MS.gene83507.t1.exon3;Parent=MS.gene83507.t1 |
| chr2.4 | CDS | 283811 | 283916 | 283811 | ID=cds.MS.gene83507.t1;Parent=MS.gene83507.t1 |
| Gene Sequence |
| Protein sequence |
