AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene050670


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene050670.t1 MTR_7g117900 93.913 230 9 1 1 230 1 225 4.83e-155 429
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene050670.t1 AT4G00895 40.945 127 61 3 65 187 75 191 3.20e-19 82.4
MS.gene050670.t1 AT4G09650 29.333 150 99 3 87 230 52 200 5.33e-14 69.3

Find 55 sgRNAs with CRISPR-Local

Find 65 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TTGGAAGAGAAATGAGATTT+TGG 0.211304 7.3:-689508 None:intergenic
ACTGAGTTTCTGCACACTTT+TGG 0.309140 7.3:-689992 None:intergenic
TCAGTGGTGCAGTGAGGGTT+AGG 0.341774 7.3:+690010 MS.gene050670:CDS
ATGTGGAACTCAAGTTGTTT+TGG 0.349249 7.3:+689924 MS.gene050670:CDS
ATGGTTTGTGTTTGAGAGGT+TGG 0.358792 7.3:-689597 None:intergenic
ATTGGTGAAGATGAAATGTT+TGG 0.381129 7.3:+689964 MS.gene050670:CDS
TGGTTTGTGTTTGAGAGGTT+GGG 0.403119 7.3:-689596 None:intergenic
TTTGAGAGGTTGGGTCTTTG+AGG 0.403625 7.3:-689587 None:intergenic
GCAGCAGCATAGCCAGTAGC+AGG 0.415721 7.3:-689646 None:intergenic
TGAAGGGCATGTGGTTGGTT+TGG 0.422427 7.3:+689822 MS.gene050670:CDS
TGTTGGTGTTGACCCTTCAT+TGG 0.430284 7.3:+689771 MS.gene050670:CDS
TTGAGAGGTTGGGTCTTTGA+GGG 0.430753 7.3:-689586 None:intergenic
ACCACCTTCCTCACCAATGA+AGG 0.445507 7.3:-689784 None:intergenic
TTGTGAAGTGATAGAATTCA+CGG 0.447151 7.3:-689444 None:intergenic
CACCCTTCCCTCGGTTCAAA+GGG 0.453751 7.3:+689696 MS.gene050670:CDS
TTCCATCACAAGCCTGCTAC+TGG 0.468777 7.3:+689634 MS.gene050670:CDS
TTGGTTGTTGAGGATGAGAA+GGG 0.471694 7.3:-689489 None:intergenic
TTTGGTTGTTGAGGATGAGA+AGG 0.473648 7.3:-689490 None:intergenic
ACACCCTTCCCTCGGTTCAA+AGG 0.483662 7.3:+689695 MS.gene050670:CDS
TAGAATTCACGGTTGGTTGT+TGG 0.484183 7.3:-689433 None:intergenic
TTGGTGAAGATGAAATGTTT+GGG 0.494512 7.3:+689965 MS.gene050670:CDS
CAGTGGTGCAGTGAGGGTTA+GGG 0.506987 7.3:+690011 MS.gene050670:CDS
TTGAACCGAGGGAAGGGTGT+TGG 0.514330 7.3:-689692 None:intergenic
TGGGTCTTTGAGGGTAGTGA+AGG 0.516568 7.3:-689577 None:intergenic
AATTCACGGTTGGTTGTTGG+TGG 0.518266 7.3:-689430 None:intergenic
AGCCAGTAGCAGGCTTGTGA+TGG 0.522904 7.3:-689636 None:intergenic
AGGATTTATGATGAATTATG+TGG 0.523605 7.3:+689907 MS.gene050670:CDS
GAAGATGGTTTGTGTTTGAG+AGG 0.530277 7.3:-689601 None:intergenic
TGGAATACTTGAGAAGAAGA+TGG 0.533703 7.3:-689616 None:intergenic
AAATTTGAAGGGCATGTGGT+TGG 0.536206 7.3:+689817 MS.gene050670:CDS
AAGTGTTGGAAGAGTTCATG+AGG 0.536714 7.3:+689887 MS.gene050670:CDS
TGACCCTTCATTGGTGAGGA+AGG 0.538931 7.3:+689780 MS.gene050670:CDS
GTGGAGCAGAGAAAATTTGA+AGG 0.540648 7.3:+689805 MS.gene050670:CDS
GGGAATTGTTGAGGAAGTGT+TGG 0.544791 7.3:+689873 MS.gene050670:CDS
ATCCCTTTGAACCGAGGGAA+GGG 0.545984 7.3:-689698 None:intergenic
GCTGATGAAGAAGAACAAGC+TGG 0.546081 7.3:+689852 MS.gene050670:CDS
AAATTTCAATCAGTTGATGT+TGG 0.559351 7.3:+689754 MS.gene050670:CDS
GAAACTCAGTGGTGCAGTGA+GGG 0.562635 7.3:+690005 MS.gene050670:CDS
AGAGCTTGAGAAAGCATCCA+TGG 0.563193 7.3:-689389 None:intergenic
ATTCACGGTTGGTTGTTGGT+GGG 0.565174 7.3:-689429 None:intergenic
TTCATTGGTGAGGAAGGTGG+TGG 0.567833 7.3:+689786 MS.gene050670:CDS
CATCCCTTTGAACCGAGGGA+AGG 0.585509 7.3:-689699 None:intergenic
CCCTTCATTGGTGAGGAAGG+TGG 0.586166 7.3:+689783 MS.gene050670:CDS
CTGATGAAGAAGAACAAGCT+GGG 0.589597 7.3:+689853 MS.gene050670:CDS
GAGAAAATTTGAAGGGCATG+TGG 0.597790 7.3:+689813 MS.gene050670:CDS
TGAACATCCCTTTGAACCGA+GGG 0.602693 7.3:-689703 None:intergenic
GTGTTGACCCTTCATTGGTG+AGG 0.604863 7.3:+689776 MS.gene050670:CDS
TTGAACATCCCTTTGAACCG+AGG 0.616637 7.3:-689704 None:intergenic
GAAGGGTTTGCAGTTGCATG+AGG 0.616977 7.3:-689472 None:intergenic
GAACAAGCTGGGAATTGTTG+AGG 0.625515 7.3:+689864 MS.gene050670:CDS
CCACCTTCCTCACCAATGAA+GGG 0.627070 7.3:-689783 None:intergenic
GAAGTGATAGAATTCACGGT+TGG 0.666630 7.3:-689440 None:intergenic
TGGAGCAGAGAAAATTTGAA+GGG 0.672649 7.3:+689806 MS.gene050670:CDS
AGAAACTCAGTGGTGCAGTG+AGG 0.695602 7.3:+690004 MS.gene050670:CDS
AAAAGTGTGCAGAAACTCAG+TGG 0.740335 7.3:+689994 MS.gene050670:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TGAGAAAGTTTTTGTTTTTT+GGG - chr7.3:689542-689561 None:intergenic 20.0%
! AGGATTTATGATGAATTATG+TGG + chr7.3:689907-689926 MS.gene050670:CDS 25.0%
! GTTTCATCAAAAAGAGAAAT+TGG + chr7.3:689946-689965 MS.gene050670:CDS 25.0%
!! AAATTTCAATCAGTTGATGT+TGG + chr7.3:689754-689773 MS.gene050670:CDS 25.0%
!! CTGATTGAAATTTCAGTTTT+TGG - chr7.3:689747-689766 None:intergenic 25.0%
!!! ATTGAAATTTCAGTTTTTGG+AGG - chr7.3:689744-689763 None:intergenic 25.0%
!!! GTGAGAAAGTTTTTGTTTTT+TGG - chr7.3:689543-689562 None:intergenic 25.0%
ATTGGTGAAGATGAAATGTT+TGG + chr7.3:689964-689983 MS.gene050670:CDS 30.0%
TTGGTGAAGATGAAATGTTT+GGG + chr7.3:689965-689984 MS.gene050670:CDS 30.0%
TTGTGAAGTGATAGAATTCA+CGG - chr7.3:689447-689466 None:intergenic 30.0%
! TTGGAAGAGAAATGAGATTT+TGG - chr7.3:689511-689530 None:intergenic 30.0%
!!! AAATGAGATTTTGGTTGTTG+AGG - chr7.3:689502-689521 None:intergenic 30.0%
GAGGGTTAGGGTTAAAAATT+TGG + chr7.3:690023-690042 MS.gene050670:CDS 35.0%
TGGAATACTTGAGAAGAAGA+TGG - chr7.3:689619-689638 None:intergenic 35.0%
TGGAGCAGAGAAAATTTGAA+GGG + chr7.3:689806-689825 MS.gene050670:CDS 35.0%
!! ATGTGGAACTCAAGTTGTTT+TGG + chr7.3:689924-689943 MS.gene050670:CDS 35.0%
!!! TGTTTTTTGGGTGACTAAGT+TGG - chr7.3:689530-689549 None:intergenic 35.0%
CTGATGAAGAAGAACAAGCT+GGG + chr7.3:689853-689872 MS.gene050670:CDS 40.0%
GAAGTGATAGAATTCACGGT+TGG - chr7.3:689443-689462 None:intergenic 40.0%
GTGGAGCAGAGAAAATTTGA+AGG + chr7.3:689805-689824 MS.gene050670:CDS 40.0%
TTGGTTGTTGAGGATGAGAA+GGG - chr7.3:689492-689511 None:intergenic 40.0%
TTTGGTTGTTGAGGATGAGA+AGG - chr7.3:689493-689512 None:intergenic 40.0%
! AAAAGTGTGCAGAAACTCAG+TGG + chr7.3:689994-690013 MS.gene050670:CDS 40.0%
! AAATTTGAAGGGCATGTGGT+TGG + chr7.3:689817-689836 MS.gene050670:CDS 40.0%
! ACTGAGTTTCTGCACACTTT+TGG - chr7.3:689995-690014 None:intergenic 40.0%
! ATGGTTTGTGTTTGAGAGGT+TGG - chr7.3:689600-689619 None:intergenic 40.0%
! GAAGATGGTTTGTGTTTGAG+AGG - chr7.3:689604-689623 None:intergenic 40.0%
! GAGAAAATTTGAAGGGCATG+TGG + chr7.3:689813-689832 MS.gene050670:CDS 40.0%
! TAGAATTCACGGTTGGTTGT+TGG - chr7.3:689436-689455 None:intergenic 40.0%
! TGGTTTGTGTTTGAGAGGTT+GGG - chr7.3:689599-689618 None:intergenic 40.0%
!! AAGTGTTGGAAGAGTTCATG+AGG + chr7.3:689887-689906 MS.gene050670:CDS 40.0%
AAAAACCAACACCCTTCCCT+CGG + chr7.3:689687-689706 MS.gene050670:CDS 45.0%
GAACAAGCTGGGAATTGTTG+AGG + chr7.3:689864-689883 MS.gene050670:CDS 45.0%
GCTGATGAAGAAGAACAAGC+TGG + chr7.3:689852-689871 MS.gene050670:CDS 45.0%
TGAACATCCCTTTGAACCGA+GGG - chr7.3:689706-689725 None:intergenic 45.0%
TTGAACATCCCTTTGAACCG+AGG - chr7.3:689707-689726 None:intergenic 45.0%
TTGAGAGGTTGGGTCTTTGA+GGG - chr7.3:689589-689608 None:intergenic 45.0%
TTTGAGAGGTTGGGTCTTTG+AGG - chr7.3:689590-689609 None:intergenic 45.0%
! AATTCACGGTTGGTTGTTGG+TGG - chr7.3:689433-689452 None:intergenic 45.0%
! ATTCACGGTTGGTTGTTGGT+GGG - chr7.3:689432-689451 None:intergenic 45.0%
! GGGAATTGTTGAGGAAGTGT+TGG + chr7.3:689873-689892 MS.gene050670:CDS 45.0%
!! GGTTGTTGGTGGGATTTTGA+GGG - chr7.3:689422-689441 None:intergenic 45.0%
!! TGGTTGTTGGTGGGATTTTG+AGG - chr7.3:689423-689442 None:intergenic 45.0%
!! TGTTGGTGTTGACCCTTCAT+TGG + chr7.3:689771-689790 MS.gene050670:CDS 45.0%
ACCACCTTCCTCACCAATGA+AGG - chr7.3:689787-689806 None:intergenic 50.0%
AGAAACTCAGTGGTGCAGTG+AGG + chr7.3:690004-690023 MS.gene050670:CDS 50.0%
ATCCCTTTGAACCGAGGGAA+GGG - chr7.3:689701-689720 None:intergenic 50.0%
CCACCTTCCTCACCAATGAA+GGG - chr7.3:689786-689805 None:intergenic 50.0%
GAAACTCAGTGGTGCAGTGA+GGG + chr7.3:690005-690024 MS.gene050670:CDS 50.0%
GAAGGGTTTGCAGTTGCATG+AGG - chr7.3:689475-689494 None:intergenic 50.0%
TGACCCTTCATTGGTGAGGA+AGG + chr7.3:689780-689799 MS.gene050670:CDS 50.0%
TTCATTGGTGAGGAAGGTGG+TGG + chr7.3:689786-689805 MS.gene050670:CDS 50.0%
TTCCATCACAAGCCTGCTAC+TGG + chr7.3:689634-689653 MS.gene050670:CDS 50.0%
! GTGTTGACCCTTCATTGGTG+AGG + chr7.3:689776-689795 MS.gene050670:CDS 50.0%
! TGAAGGGCATGTGGTTGGTT+TGG + chr7.3:689822-689841 MS.gene050670:CDS 50.0%
! TGGGTCTTTGAGGGTAGTGA+AGG - chr7.3:689580-689599 None:intergenic 50.0%
ACACCCTTCCCTCGGTTCAA+AGG + chr7.3:689695-689714 MS.gene050670:CDS 55.0%
AGCCAGTAGCAGGCTTGTGA+TGG - chr7.3:689639-689658 None:intergenic 55.0%
CACCCTTCCCTCGGTTCAAA+GGG + chr7.3:689696-689715 MS.gene050670:CDS 55.0%
CAGTGGTGCAGTGAGGGTTA+GGG + chr7.3:690011-690030 MS.gene050670:CDS 55.0%
CATCCCTTTGAACCGAGGGA+AGG - chr7.3:689702-689721 None:intergenic 55.0%
CCCTTCATTGGTGAGGAAGG+TGG + chr7.3:689783-689802 MS.gene050670:CDS 55.0%
TCAGTGGTGCAGTGAGGGTT+AGG + chr7.3:690010-690029 MS.gene050670:CDS 55.0%
! TTGAACCGAGGGAAGGGTGT+TGG - chr7.3:689695-689714 None:intergenic 55.0%
GCAGCAGCATAGCCAGTAGC+AGG - chr7.3:689649-689668 None:intergenic 60.0%


Chromosome Type Strat End Strand Name
chr7.3 gene 689391 690083 689391 ID=MS.gene050670
chr7.3 mRNA 689391 690083 689391 ID=MS.gene050670.t1;Parent=MS.gene050670
chr7.3 exon 689391 690083 689391 ID=MS.gene050670.t1.exon1;Parent=MS.gene050670.t1
chr7.3 CDS 689391 690083 689391 ID=cds.MS.gene050670.t1;Parent=MS.gene050670.t1
Gene Sequence

>MS.gene050670

ATGGATGCTTTCTCAAGCTCTGTTTCAACCCTCAAAATCCCACCAACAACCAACCGTGAATTCTATCACTTCACAAGAACTCCTCATGCAACTGCAAACCCTTCTCATCCTCAACAACCAAAATCTCATTTCTCTTCCAACTTAGTCACCCAAAAAACAAAAACTTTCTCACACAACAATCATTCTCCTTCACTACCCTCAAAGACCCAACCTCTCAAACACAAACCATCTTCTTCTCAAGTATTCCATCACAAGCCTGCTACTGGCTATGCTGCTGCAATCATAGATGTAGCTCAAAAAACCAACACCCTTCCCTCGGTTCAAAGGGATGTTCAAAGACTCTTGAAGTTCCTCCAAAAACTGAAATTTCAATCAGTTGATGTTGGTGTTGACCCTTCATTGGTGAGGAAGGTGGTGGAGCAGAGAAAATTTGAAGGGCATGTGGTTGGTTTGGTGAAGATGCTGATGAAGAAGAACAAGCTGGGAATTGTTGAGGAAGTGTTGGAAGAGTTCATGAGGATTTATGATGAATTATGTGGAACTCAAGTTGTTTTGGTTTCATCAAAAAGAGAAATTGGTGAAGATGAAATGTTTGGGATTGCCAAAAGTGTGCAGAAACTCAGTGGTGCAGTGAGGGTTAGGGTTAAAAATTTGGTTCAAGAAGAGAGTTTTCCTTCTTCTTTTGCAGTGTAA

Protein sequence

>MS.gene050670.t1

MDAFSSSVSTLKIPPTTNREFYHFTRTPHATANPSHPQQPKSHFSSNLVTQKTKTFSHNNHSPSLPSKTQPLKHKPSSSQVFHHKPATGYAAAIIDVAQKTNTLPSVQRDVQRLLKFLQKLKFQSVDVGVDPSLVRKVVEQRKFEGHVVGLVKMLMKKNKLGIVEEVLEEFMRIYDELCGTQVVLVSSKREIGEDEMFGIAKSVQKLSGAVRVRVKNLVQEESFPSSFAV