Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene83692.t1 | KEH29493.1 | 67 | 97 | 31 | 1 | 1 | 97 | 1 | 96 | 3.50E-23 | 117.5 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene83692.t1 | A0A072UK96 | 67.0 | 97 | 31 | 1 | 1 | 97 | 1 | 96 | 2.5e-23 | 117.5 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene050521 | MS.gene83692 | 0.821612 | 3.68E-53 | -1.69E-46 |
| MS.gene060076 | MS.gene83692 | 0.80037 | 1.49E-48 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene83692.t1 | MTR_4g037645 | 79.798 | 99 | 15 | 2 | 1 | 97 | 1 | 96 | 3.62e-49 | 151 |
| MS.gene83692.t1 | MTR_4g037615 | 78.788 | 99 | 16 | 2 | 1 | 97 | 1 | 96 | 1.07e-46 | 145 |
| MS.gene83692.t1 | MTR_4g037655 | 57.143 | 98 | 17 | 2 | 1 | 97 | 1 | 74 | 4.27e-26 | 92.4 |
| MS.gene83692.t1 | MTR_7g028468 | 46.392 | 97 | 33 | 2 | 1 | 97 | 1 | 78 | 1.84e-20 | 78.2 |
| MS.gene83692.t1 | MTR_4g036950 | 46.392 | 97 | 33 | 2 | 1 | 97 | 1 | 78 | 1.84e-20 | 78.2 |
| MS.gene83692.t1 | MTR_7g028465 | 47.917 | 96 | 29 | 2 | 1 | 96 | 1 | 75 | 3.08e-19 | 75.1 |
| MS.gene83692.t1 | MTR_4g036945 | 47.917 | 96 | 29 | 2 | 1 | 96 | 1 | 75 | 3.08e-19 | 75.1 |
| MS.gene83692.t1 | MTR_8g070055 | 38.776 | 98 | 45 | 2 | 1 | 97 | 1 | 84 | 2.94e-17 | 70.1 |
| MS.gene83692.t1 | MTR_7g028435 | 40.179 | 112 | 45 | 5 | 1 | 97 | 1 | 105 | 3.05e-17 | 70.9 |
| MS.gene83692.t1 | MTR_4g037005 | 40.179 | 112 | 45 | 5 | 1 | 97 | 1 | 105 | 3.05e-17 | 70.9 |
| MS.gene83692.t1 | MTR_7g028462 | 43.299 | 97 | 33 | 2 | 1 | 97 | 1 | 75 | 2.77e-15 | 65.1 |
| MS.gene83692.t1 | MTR_4g036935 | 43.299 | 97 | 33 | 2 | 1 | 97 | 1 | 75 | 2.77e-15 | 65.1 |
| MS.gene83692.t1 | MTR_4g037005 | 37.500 | 112 | 46 | 6 | 1 | 97 | 1 | 103 | 2.18e-13 | 60.8 |
| MS.gene83692.t1 | MTR_7g028442 | 37.755 | 98 | 40 | 2 | 1 | 97 | 1 | 78 | 2.91e-12 | 57.4 |
| MS.gene83692.t1 | MTR_4g036880 | 37.755 | 98 | 40 | 2 | 1 | 97 | 1 | 78 | 2.91e-12 | 57.4 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 16 sgRNAs with CRISPR-Local
Find 44 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| AAAGAAGTGCTCTCTTATTT+TGG | 0.217478 | 4.3:-62056525 | MS.gene83692:CDS |
| TTGTGGCCACGTGCTTTATC+AGG | 0.279751 | 4.3:+62056256 | None:intergenic |
| TGTATAGTTTCAGATGAAAT+AGG | 0.330215 | 4.3:-62056313 | MS.gene83692:intron |
| AATAAGAAGAGACTCCTTGC+TGG | 0.444927 | 4.3:-62056286 | MS.gene83692:CDS |
| GACTCCTTGCAGAAAATTGT+GGG | 0.487595 | 4.3:-62055850 | MS.gene83692:CDS |
| AGACTCCTTGCAGAAAATTG+TGG | 0.531866 | 4.3:-62055851 | MS.gene83692:CDS |
| AAGTAAAACCTGCAAAATTG+TGG | 0.541805 | 4.3:+62056239 | None:intergenic |
| TTGCAGAAAATTGTGGGAGT+AGG | 0.564611 | 4.3:-62055844 | MS.gene83692:CDS |
| TACATGATCCTCATGCTGAT+CGG | 0.593858 | 4.3:-62055820 | MS.gene83692:CDS |
| TTTCATCAAAGACATTAGCA+AGG | 0.595257 | 4.3:-62056475 | MS.gene83692:CDS |
| ATATCATTCATAGAAAATTG+TGG | 0.617375 | 4.3:+62055789 | None:intergenic |
| AGAAGAGACTCCTTGCTGGT+AGG | 0.629830 | 4.3:-62056282 | MS.gene83692:CDS |
| TATCAGGCTTCCTACCAGCA+AGG | 0.630061 | 4.3:+62056272 | None:intergenic |
| CAACGTGGCCGATCAGCATG+AGG | 0.647834 | 4.3:+62055812 | None:intergenic |
| CATAGAAAATTGTGGCAACG+TGG | 0.674818 | 4.3:+62055797 | None:intergenic |
| AGGAAGCCTGATAAAGCACG+TGG | 0.728123 | 4.3:-62056262 | MS.gene83692:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !!! | TATTTTGATAAAAGAAAAAT+GGG | - | chr4.3:62056352-62056371 | MS.gene83692:intron | 10.0% |
| !!! | TTATTTTGATAAAAGAAAAA+TGG | - | chr4.3:62056351-62056370 | MS.gene83692:intron | 10.0% |
| !!! | TTTTTTCTTGTATTAATATT+TGG | - | chr4.3:62056413-62056432 | MS.gene83692:intron | 10.0% |
| !! | AATAACAAAATGGATATTAT+GGG | + | chr4.3:62055924-62055943 | None:intergenic | 15.0% |
| !! | AGTATTATTTATGGATATTA+TGG | + | chr4.3:62056118-62056137 | None:intergenic | 15.0% |
| !! | GTATTATTTATGGATATTAT+GGG | + | chr4.3:62056117-62056136 | None:intergenic | 15.0% |
| !! | TATTATTTATGGATATTATG+GGG | + | chr4.3:62056116-62056135 | None:intergenic | 15.0% |
| !! | ATAACAAAATGGATATTATG+GGG | + | chr4.3:62055923-62055942 | None:intergenic | 20.0% |
| !! | CAATAACAAAATGGATATTA+TGG | + | chr4.3:62055925-62055944 | None:intergenic | 20.0% |
| !! | TGGATAAAGTTCAATAAATA+AGG | - | chr4.3:62056276-62056295 | MS.gene83692:CDS | 20.0% |
| ! | AAACGACTATTATTTAGAGA+CGG | + | chr4.3:62056145-62056164 | None:intergenic | 25.0% |
| ! | AAAGAAACAATTATTGTGAG+CGG | - | chr4.3:62056376-62056395 | MS.gene83692:intron | 25.0% |
| ! | CTCTAAATAATAGTCGTTTA+AGG | - | chr4.3:62056146-62056165 | MS.gene83692:intron | 25.0% |
| ! | TCCACTAACTACAATTAATA+AGG | + | chr4.3:62056246-62056265 | None:intergenic | 25.0% |
| ! | TCCTTATTAATTGTAGTTAG+TGG | - | chr4.3:62056242-62056261 | MS.gene83692:intron | 25.0% |
| ! | TGTATAGTTTCAGATGAAAT+AGG | - | chr4.3:62056016-62056035 | MS.gene83692:intron | 25.0% |
| AACGACTATTATTTAGAGAC+GGG | + | chr4.3:62056144-62056163 | None:intergenic | 30.0% | |
| AAGTAAAACCTGCAAAATTG+TGG | + | chr4.3:62056093-62056112 | None:intergenic | 30.0% | |
| TTTCATCAAAGACATTAGCA+AGG | - | chr4.3:62055854-62055873 | MS.gene83692:CDS | 30.0% | |
| ! | GTTGTTCTTGTATTGATGTA+TGG | - | chr4.3:62055987-62056006 | MS.gene83692:intron | 30.0% |
| !!! | AAAGAAGTGCTCTCTTATTT+TGG | - | chr4.3:62055804-62055823 | MS.gene83692:CDS | 30.0% |
| AAACAATTATTGTGAGCGGA+GGG | - | chr4.3:62056380-62056399 | MS.gene83692:intron | 35.0% | |
| ACGACTATTATTTAGAGACG+GGG | + | chr4.3:62056143-62056162 | None:intergenic | 35.0% | |
| AGTTAGTGGAGTAGTTAAGT+TGG | - | chr4.3:62056256-62056275 | MS.gene83692:CDS | 35.0% | |
| GCGCAAATGCAATAACAAAA+TGG | + | chr4.3:62055934-62055953 | None:intergenic | 35.0% | |
| AATAAGAAGAGACTCCTTGC+TGG | - | chr4.3:62056043-62056062 | MS.gene83692:intron | 40.0% | |
| AGACTCCTTGCAGAAAATTG+TGG | - | chr4.3:62056478-62056497 | MS.gene83692:CDS | 40.0% | |
| CATAGAAAATTGTGGCAACG+TGG | + | chr4.3:62056535-62056554 | None:intergenic | 40.0% | |
| CGACTATTATTTAGAGACGG+GGG | + | chr4.3:62056142-62056161 | None:intergenic | 40.0% | |
| GAAACAATTATTGTGAGCGG+AGG | - | chr4.3:62056379-62056398 | MS.gene83692:intron | 40.0% | |
| GACTATTATTTAGAGACGGG+GGG | + | chr4.3:62056141-62056160 | None:intergenic | 40.0% | |
| GACTCCTTGCAGAAAATTGT+GGG | - | chr4.3:62056479-62056498 | MS.gene83692:CDS | 40.0% | |
| TACATGATCCTCATGCTGAT+CGG | - | chr4.3:62056509-62056528 | MS.gene83692:CDS | 40.0% | |
| TTGCAGAAAATTGTGGGAGT+AGG | - | chr4.3:62056485-62056504 | MS.gene83692:CDS | 40.0% | |
| ! | TACTCCCACAATTTTCTGCA+AGG | + | chr4.3:62056486-62056505 | None:intergenic | 40.0% |
| TATGGGGAAGTTCACGTACT+TGG | + | chr4.3:62055907-62055926 | None:intergenic | 45.0% | |
| !! | GACGGGGGGAGTATTATTTA+TGG | + | chr4.3:62056127-62056146 | None:intergenic | 45.0% |
| !! | TATAATGAAAAAAATAATAA+AGG | - | chr4.3:62056303-62056322 | MS.gene83692:CDS | 5.0% |
| AGAAGAGACTCCTTGCTGGT+AGG | - | chr4.3:62056047-62056066 | MS.gene83692:intron | 50.0% | |
| AGGAAGCCTGATAAAGCACG+TGG | - | chr4.3:62056067-62056086 | MS.gene83692:intron | 50.0% | |
| TATCAGGCTTCCTACCAGCA+AGG | + | chr4.3:62056060-62056079 | None:intergenic | 50.0% | |
| TTGTGGCCACGTGCTTTATC+AGG | + | chr4.3:62056076-62056095 | None:intergenic | 50.0% | |
| ! | GCACGTGGCCACAATTTTGC+AGG | - | chr4.3:62056082-62056101 | MS.gene83692:intron | 55.0% |
| CAACGTGGCCGATCAGCATG+AGG | + | chr4.3:62056520-62056539 | None:intergenic | 60.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr4.3 | gene | 62055796 | 62056555 | 62055796 | ID=MS.gene83692 |
| chr4.3 | mRNA | 62055796 | 62056555 | 62055796 | ID=MS.gene83692.t1;Parent=MS.gene83692 |
| chr4.3 | exon | 62056444 | 62056555 | 62056444 | ID=MS.gene83692.t1.exon1;Parent=MS.gene83692.t1 |
| chr4.3 | CDS | 62056444 | 62056555 | 62056444 | ID=cds.MS.gene83692.t1;Parent=MS.gene83692.t1 |
| chr4.3 | exon | 62056248 | 62056328 | 62056248 | ID=MS.gene83692.t1.exon2;Parent=MS.gene83692.t1 |
| chr4.3 | CDS | 62056248 | 62056328 | 62056248 | ID=cds.MS.gene83692.t1;Parent=MS.gene83692.t1 |
| chr4.3 | exon | 62055796 | 62055896 | 62055796 | ID=MS.gene83692.t1.exon3;Parent=MS.gene83692.t1 |
| chr4.3 | CDS | 62055796 | 62055896 | 62055796 | ID=cds.MS.gene83692.t1;Parent=MS.gene83692.t1 |
| Gene Sequence |
| Protein sequence |
