Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene84044.t1 | XP_003590250.1 | 87.7 | 569 | 56 | 1 | 1 | 555 | 27 | 595 | 2.50E-254 | 887.9 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene84044.t1 | F4I9E1 | 22.6 | 532 | 388 | 12 | 1 | 523 | 46 | 562 | 4.3e-21 | 104.4 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene84044.t1 | G7I7D1 | 87.7 | 569 | 56 | 1 | 1 | 555 | 27 | 595 | 1.8e-254 | 887.9 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene055909 | MS.gene84044 | 0.806551 | 7.79E-50 | -1.69E-46 |
| MS.gene055911 | MS.gene84044 | 0.801005 | 1.10E-48 | -1.69E-46 |
| MS.gene057749 | MS.gene84044 | 0.807132 | 5.87E-50 | -1.69E-46 |
| MS.gene057823 | MS.gene84044 | 0.810622 | 1.05E-50 | -1.69E-46 |
| MS.gene059420 | MS.gene84044 | 0.802024 | 6.83E-49 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene84044.t1 | MTR_1g050550 | 95.079 | 569 | 14 | 2 | 1 | 555 | 27 | 595 | 0.0 | 1090 |
| MS.gene84044.t1 | MTR_1g059080 | 86.348 | 564 | 66 | 2 | 1 | 556 | 27 | 587 | 0.0 | 954 |
| MS.gene84044.t1 | MTR_1g111510 | 59.541 | 566 | 217 | 3 | 1 | 556 | 29 | 592 | 0.0 | 696 |
| MS.gene84044.t1 | MTR_1g111540 | 60.071 | 561 | 211 | 4 | 1 | 555 | 29 | 582 | 0.0 | 689 |
| MS.gene84044.t1 | MTR_1g111530 | 59.854 | 548 | 211 | 2 | 1 | 541 | 25 | 570 | 0.0 | 682 |
| MS.gene84044.t1 | MTR_1g111550 | 58.979 | 568 | 216 | 5 | 1 | 556 | 28 | 590 | 0.0 | 674 |
| MS.gene84044.t1 | MTR_7g102880 | 55.712 | 569 | 238 | 4 | 1 | 555 | 32 | 600 | 0.0 | 634 |
| MS.gene84044.t1 | MTR_2g100400 | 34.291 | 557 | 318 | 7 | 2 | 536 | 11 | 541 | 2.20e-108 | 335 |
| MS.gene84044.t1 | MTR_5g090570 | 35.502 | 538 | 318 | 5 | 4 | 533 | 17 | 533 | 1.95e-105 | 327 |
| MS.gene84044.t1 | MTR_7g029015 | 35.398 | 565 | 316 | 13 | 8 | 530 | 25 | 582 | 3.88e-100 | 315 |
| MS.gene84044.t1 | MTR_3g099150 | 34.082 | 534 | 329 | 7 | 9 | 524 | 26 | 554 | 1.28e-93 | 297 |
| MS.gene84044.t1 | MTR_4g073690 | 34.848 | 528 | 323 | 6 | 4 | 524 | 21 | 534 | 5.42e-88 | 282 |
| MS.gene84044.t1 | MTR_2g450710 | 32.463 | 536 | 331 | 9 | 12 | 524 | 26 | 553 | 2.89e-82 | 268 |
| MS.gene84044.t1 | MTR_5g022380 | 31.136 | 546 | 329 | 10 | 4 | 537 | 19 | 529 | 3.93e-82 | 266 |
| MS.gene84044.t1 | MTR_4g116210 | 31.009 | 545 | 332 | 11 | 13 | 524 | 26 | 559 | 4.30e-80 | 262 |
| MS.gene84044.t1 | MTR_5g022380 | 30.176 | 454 | 270 | 10 | 96 | 537 | 1 | 419 | 1.16e-60 | 207 |
| MS.gene84044.t1 | MTR_4g073690 | 33.333 | 378 | 240 | 5 | 4 | 374 | 21 | 393 | 1.64e-52 | 184 |
| MS.gene84044.t1 | MTR_3g466040 | 27.115 | 520 | 351 | 10 | 8 | 523 | 17 | 512 | 3.60e-37 | 144 |
| MS.gene84044.t1 | MTR_3g466110 | 25.309 | 486 | 331 | 8 | 8 | 481 | 17 | 482 | 3.91e-34 | 135 |
| MS.gene84044.t1 | MTR_7g083660 | 25.528 | 568 | 377 | 13 | 1 | 538 | 10 | 561 | 4.27e-32 | 130 |
| MS.gene84044.t1 | MTR_5g026350 | 25.565 | 575 | 374 | 19 | 1 | 536 | 10 | 569 | 1.05e-26 | 114 |
| MS.gene84044.t1 | MTR_2g104250 | 24.684 | 316 | 179 | 8 | 225 | 524 | 17 | 289 | 1.35e-25 | 107 |
| MS.gene84044.t1 | MTR_5g095240 | 59.155 | 71 | 29 | 0 | 460 | 530 | 2 | 72 | 9.73e-23 | 92.4 |
| MS.gene84044.t1 | MTR_8g071920 | 28.821 | 229 | 151 | 7 | 299 | 524 | 381 | 600 | 1.54e-11 | 67.4 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene84044.t1 | AT2G39210 | 60.917 | 545 | 200 | 3 | 1 | 534 | 24 | 566 | 0.0 | 694 |
| MS.gene84044.t1 | AT2G28120 | 58.018 | 555 | 222 | 3 | 1 | 548 | 20 | 570 | 0.0 | 659 |
| MS.gene84044.t1 | AT1G74780 | 37.294 | 547 | 301 | 6 | 4 | 534 | 13 | 533 | 8.53e-115 | 351 |
| MS.gene84044.t1 | AT2G34350 | 35.250 | 539 | 308 | 7 | 4 | 530 | 13 | 522 | 1.75e-103 | 322 |
| MS.gene84044.t1 | AT2G34350 | 35.250 | 539 | 308 | 7 | 4 | 530 | 13 | 522 | 1.75e-103 | 322 |
| MS.gene84044.t1 | AT1G18940 | 35.860 | 541 | 299 | 7 | 4 | 530 | 15 | 521 | 4.58e-103 | 320 |
| MS.gene84044.t1 | AT5G50520 | 34.470 | 528 | 326 | 7 | 1 | 523 | 18 | 530 | 1.32e-100 | 315 |
| MS.gene84044.t1 | AT5G50630 | 34.470 | 528 | 326 | 7 | 1 | 523 | 18 | 530 | 1.32e-100 | 315 |
| MS.gene84044.t1 | AT5G14120 | 34.331 | 568 | 330 | 11 | 1 | 532 | 18 | 578 | 3.93e-100 | 315 |
| MS.gene84044.t1 | AT2G34355 | 34.451 | 537 | 313 | 7 | 4 | 530 | 13 | 520 | 3.52e-99 | 310 |
| MS.gene84044.t1 | AT3G01930 | 32.635 | 573 | 338 | 9 | 1 | 532 | 18 | 583 | 2.47e-96 | 305 |
| MS.gene84044.t1 | AT2G16660 | 35.182 | 523 | 317 | 6 | 8 | 524 | 24 | 530 | 1.25e-92 | 294 |
| MS.gene84044.t1 | AT4G34950 | 33.633 | 556 | 327 | 8 | 8 | 537 | 26 | 565 | 1.56e-90 | 289 |
| MS.gene84044.t1 | AT1G80530 | 30.168 | 537 | 334 | 9 | 12 | 525 | 27 | 545 | 6.00e-75 | 248 |
| MS.gene84044.t1 | AT3G01930 | 30.818 | 477 | 283 | 8 | 96 | 532 | 1 | 470 | 3.88e-69 | 230 |
| MS.gene84044.t1 | AT2G34355 | 32.663 | 398 | 237 | 6 | 135 | 530 | 2 | 370 | 2.88e-64 | 214 |
| MS.gene84044.t1 | AT3G01930 | 31.754 | 422 | 243 | 7 | 1 | 381 | 18 | 435 | 5.01e-60 | 205 |
| MS.gene84044.t1 | AT2G16660 | 33.780 | 373 | 234 | 5 | 8 | 374 | 24 | 389 | 4.62e-54 | 188 |
| MS.gene84044.t1 | AT3G01630 | 25.277 | 542 | 358 | 16 | 4 | 524 | 47 | 562 | 2.74e-31 | 128 |
| MS.gene84044.t1 | AT3G01630 | 25.501 | 549 | 348 | 16 | 4 | 524 | 29 | 544 | 4.17e-31 | 127 |
| MS.gene84044.t1 | AT4G19450 | 25.907 | 579 | 362 | 19 | 1 | 536 | 10 | 564 | 4.45e-30 | 124 |
| MS.gene84044.t1 | AT1G31470 | 25.185 | 540 | 360 | 15 | 4 | 524 | 49 | 563 | 1.19e-27 | 117 |
| MS.gene84044.t1 | AT5G45275 | 24.343 | 571 | 376 | 14 | 1 | 534 | 10 | 561 | 5.15e-27 | 115 |
| MS.gene84044.t1 | AT2G30300 | 24.670 | 531 | 334 | 13 | 8 | 523 | 17 | 496 | 2.24e-26 | 113 |
| MS.gene84044.t1 | AT4G19450 | 24.384 | 406 | 254 | 12 | 1 | 364 | 10 | 404 | 9.01e-21 | 95.5 |
Find 126 sgRNAs with CRISPR-Local
Find 136 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| CCCTCTTGTTGTTGTTATTA+TGG | 0.134860 | 1.3:-32572115 | MS.gene84044:CDS |
| ATCACTGCTGTTAGCCTCTT+AGG | 0.193163 | 1.3:-32571325 | MS.gene84044:CDS |
| CTAATGCTGCCATTTGCTTT+AGG | 0.212154 | 1.3:+32571419 | None:intergenic |
| AAGAGAAATCAAATCAGTTT+TGG | 0.212226 | 1.3:-32572760 | MS.gene84044:CDS |
| ATATTCCATATTGGAGGTTT+AGG | 0.223435 | 1.3:+32572561 | None:intergenic |
| TAATGCTGCCATTTGCTTTA+GGG | 0.225798 | 1.3:+32571420 | None:intergenic |
| GTAAATAACTTGAGTCAAAT+TGG | 0.246330 | 1.3:-32571835 | MS.gene84044:CDS |
| TACATGTCTTTAATCCTTGC+AGG | 0.247805 | 1.3:-32572234 | MS.gene84044:CDS |
| CTTGAGCTTCTTCAAGGACT+TGG | 0.255144 | 1.3:-32572712 | MS.gene84044:CDS |
| AGTCAAATTGGATTATCTTT+AGG | 0.269385 | 1.3:-32571823 | MS.gene84044:CDS |
| GGTCATACTTGTTAAGTGTT+AGG | 0.272102 | 1.3:-32571471 | MS.gene84044:CDS |
| TTGTTAAGTGTTAGGGTTGC+TGG | 0.279418 | 1.3:-32571463 | MS.gene84044:CDS |
| CAACTATACTATGCCTTCTA+TGG | 0.279543 | 1.3:-32572390 | MS.gene84044:CDS |
| TATTCCATATTGGAGGTTTA+GGG | 0.301426 | 1.3:+32572562 | None:intergenic |
| TTGGTTGTTCAGCTCTCTTA+TGG | 0.310124 | 1.3:+32572286 | None:intergenic |
| CACTCTCTTGAGCTTCTTCA+AGG | 0.315653 | 1.3:-32572718 | MS.gene84044:CDS |
| GGTTCTGCTTTGGTGCAAAT+TGG | 0.316928 | 1.3:-32571582 | MS.gene84044:CDS |
| TCAATCATATCTGAACTCTT+TGG | 0.320938 | 1.3:-32571547 | MS.gene84044:CDS |
| TTTGGAGTCATTTCCATAGA+AGG | 0.324553 | 1.3:+32572377 | None:intergenic |
| CATAAGTAAAATTAAAGATT+TGG | 0.327529 | 1.3:+32572359 | None:intergenic |
| CTAAAGCAAATGGCAGCATT+AGG | 0.335582 | 1.3:-32571418 | MS.gene84044:CDS |
| CATCATGTCAAGGCTGAAAA+TGG | 0.337307 | 1.3:+32571906 | None:intergenic |
| TGTCTCTAACTTTGGTGTTA+AGG | 0.341183 | 1.3:-32571294 | MS.gene84044:CDS |
| GTCATACTTGTTAAGTGTTA+GGG | 0.342527 | 1.3:-32571470 | MS.gene84044:CDS |
| CCTTGGGTTGTCTTAACAAT+TGG | 0.343987 | 1.3:-32572642 | MS.gene84044:CDS |
| TTTATGATCATGTCAGTATC+TGG | 0.347170 | 1.3:-32572810 | MS.gene84044:CDS |
| GGACATTAAATGCAATTAGA+AGG | 0.350651 | 1.3:+32571638 | None:intergenic |
| GATAGATCCATATTGCCATT+AGG | 0.350724 | 1.3:+32571767 | None:intergenic |
| CTCTTATGGTGCTTAATAAC+TGG | 0.351299 | 1.3:+32572300 | None:intergenic |
| TTATGGAAGAACAAAGGATT+TGG | 0.359352 | 1.3:-32572098 | MS.gene84044:CDS |
| GATTGGTGAGTCTGTGGCTT+AGG | 0.360111 | 1.3:+32572036 | None:intergenic |
| AAATCCCTAAACCTCCAATA+TGG | 0.365760 | 1.3:-32572566 | MS.gene84044:CDS |
| GATGCTACATAAAGACCATT+TGG | 0.367090 | 1.3:+32571616 | None:intergenic |
| TTGTGTCCCTAATGGCAATA+TGG | 0.370362 | 1.3:-32571774 | MS.gene84044:CDS |
| TAGAAATAATGAAACTGAAT+TGG | 0.371893 | 1.3:-32571221 | MS.gene84044:CDS |
| GGGTCAATAGCAAGTCCAAT+AGG | 0.385410 | 1.3:-32571493 | MS.gene84044:CDS |
| TGGAAGTCAGTGCTACAAAT+TGG | 0.387074 | 1.3:-32571359 | MS.gene84044:CDS |
| GAGCAAGAGAAGCATCAAAA+TGG | 0.387481 | 1.3:+32572143 | None:intergenic |
| ATTATTGTTGGGTTCTGCTT+TGG | 0.389086 | 1.3:-32571592 | MS.gene84044:CDS |
| TTGAGCTTCTTCAAGGACTT+GGG | 0.389162 | 1.3:-32572711 | MS.gene84044:CDS |
| GGCACTTGTGTCTCTAACTT+TGG | 0.390758 | 1.3:-32571302 | MS.gene84044:CDS |
| ATAGATCCATATTGCCATTA+GGG | 0.394821 | 1.3:+32571768 | None:intergenic |
| TTAGGGTTAAAGAGGAAGTC+AGG | 0.397274 | 1.3:-32571400 | MS.gene84044:CDS |
| TGGGTTGTCTTAACAATTGG+TGG | 0.401882 | 1.3:-32572639 | MS.gene84044:CDS |
| TCACTGCTGTTAGCCTCTTA+GGG | 0.413238 | 1.3:-32571324 | MS.gene84044:CDS |
| CTTAACAAGTATGACCCTAT+TGG | 0.415970 | 1.3:+32571478 | None:intergenic |
| TATAAGCACATATTCCATAT+TGG | 0.421719 | 1.3:+32572552 | None:intergenic |
| GTATGATCTTCACCTCTACT+TGG | 0.422261 | 1.3:+32571940 | None:intergenic |
| ATGTAGCATCAATTATTGTT+GGG | 0.422388 | 1.3:-32571603 | MS.gene84044:CDS |
| TACTCAACATTGTATAATGT+TGG | 0.424470 | 1.3:-32571514 | MS.gene84044:CDS |
| ATCCTTATCTTGTCTTGTGT+TGG | 0.425495 | 1.3:-32571664 | MS.gene84044:CDS |
| CAATGAAATCACACCTCCTT+GGG | 0.429265 | 1.3:-32572658 | MS.gene84044:CDS |
| ATGTGCTTATACATATTCAT+TGG | 0.430843 | 1.3:-32572540 | MS.gene84044:CDS |
| GAGTAGTCTCTCCTCTAGAT+TGG | 0.434002 | 1.3:+32572019 | None:intergenic |
| TATGTAGCATCAATTATTGT+TGG | 0.439327 | 1.3:-32571604 | MS.gene84044:CDS |
| TCTGGAGCAAGCTACATGTT+TGG | 0.448168 | 1.3:-32572792 | MS.gene84044:CDS |
| ATGGCAATATGGATCTATCT+AGG | 0.449418 | 1.3:-32571763 | MS.gene84044:CDS |
| ACTCAACATTGTATAATGTT+GGG | 0.455480 | 1.3:-32571513 | MS.gene84044:CDS |
| GGTAGTAGAGGTGTTGTCAT+AGG | 0.459692 | 1.3:-32572456 | MS.gene84044:CDS |
| ATGCTACATAAAGACCATTT+GGG | 0.460886 | 1.3:+32571617 | None:intergenic |
| TAACAAAGTTAAAACTTCCT+AGG | 0.465000 | 1.3:-32571705 | MS.gene84044:CDS |
| TAAAGCAAATGGCAGCATTA+GGG | 0.468002 | 1.3:-32571417 | MS.gene84044:CDS |
| ATTGCATTTAATGTCCCAAA+TGG | 0.471433 | 1.3:-32571631 | MS.gene84044:CDS |
| TCAATGAAATCACACCTCCT+TGG | 0.473514 | 1.3:-32572659 | MS.gene84044:CDS |
| TATGATCTTCACCTCTACTT+GGG | 0.477700 | 1.3:+32571941 | None:intergenic |
| AACCACATTTGTGTCCCTAA+TGG | 0.479854 | 1.3:-32571782 | MS.gene84044:CDS |
| TTCACCTCTACTTGGGGAAG+AGG | 0.481674 | 1.3:+32571948 | None:intergenic |
| CAAAGAAGCCCTAAAGCAAA+TGG | 0.484783 | 1.3:-32571428 | MS.gene84044:CDS |
| ATCTGTCTATGCATCATTTG+AGG | 0.496384 | 1.3:+32571171 | None:intergenic |
| GATGTGAGCATGAGAGGCCT+AGG | 0.496391 | 1.3:+32571688 | None:intergenic |
| ACATGTCTTTAATCCTTGCA+GGG | 0.496948 | 1.3:-32572233 | MS.gene84044:CDS |
| GACCAACACAAGACAAGATA+AGG | 0.506863 | 1.3:+32571662 | None:intergenic |
| TGAGCTTCTTCAAGGACTTG+GGG | 0.508600 | 1.3:-32572710 | MS.gene84044:CDS |
| CAAGGATTAAAGACATGTAA+AGG | 0.512316 | 1.3:+32572238 | None:intergenic |
| TGGCAGCATTAGGGTTAAAG+AGG | 0.518733 | 1.3:-32571408 | MS.gene84044:CDS |
| AAGCACATATTCCATATTGG+AGG | 0.518894 | 1.3:+32572555 | None:intergenic |
| GTCTATGCATCATTTGAGGC+AGG | 0.522205 | 1.3:+32571175 | None:intergenic |
| AGTGGTTATCTTGGTTTGAG+TGG | 0.525713 | 1.3:-32572426 | MS.gene84044:CDS |
| AAAACAAAAGTTACAGCAGT+TGG | 0.525937 | 1.3:+32572327 | None:intergenic |
| GATATGATTGAAAATAGTAC+TGG | 0.527060 | 1.3:+32571559 | None:intergenic |
| TGGTGAGTCTGTGGCTTAGG+AGG | 0.531187 | 1.3:+32572039 | None:intergenic |
| AACCAAGATAACCACTCAAA+AGG | 0.532585 | 1.3:+32572433 | None:intergenic |
| TGAGTCTGTGGCTTAGGAGG+AGG | 0.538831 | 1.3:+32572042 | None:intergenic |
| AGGATTGATGTGAGCATGAG+AGG | 0.545649 | 1.3:+32571682 | None:intergenic |
| CCTCTAGATTGGTGAGTCTG+TGG | 0.548972 | 1.3:+32572030 | None:intergenic |
| TAGGGTTAAAGAGGAAGTCA+GGG | 0.549788 | 1.3:-32571399 | MS.gene84044:CDS |
| TCCATAATAACAACAACAAG+AGG | 0.549935 | 1.3:+32572114 | None:intergenic |
| GTAGCCACAATATGTGGACT+TGG | 0.551117 | 1.3:-32571874 | MS.gene84044:CDS |
| TCTAACATAGGCATTCTCTC+AGG | 0.553301 | 1.3:-32572687 | MS.gene84044:CDS |
| GGTCAATAGCAAGTCCAATA+GGG | 0.554065 | 1.3:-32571492 | MS.gene84044:CDS |
| GTTGTGACTCAGAACAAGGT+TGG | 0.560023 | 1.3:-32571199 | MS.gene84044:CDS |
| AAGAATTTCCCTGGTAGTAG+AGG | 0.560197 | 1.3:-32572468 | MS.gene84044:CDS |
| GAGACACAAGTGCCCCTAAG+AGG | 0.560359 | 1.3:+32571311 | None:intergenic |
| GAAGCTCAAGAGAGTGAGAG+TGG | 0.562224 | 1.3:+32572725 | None:intergenic |
| GGGGAGGAGTTGAATTGCAA+TGG | 0.565523 | 1.3:-32571379 | MS.gene84044:CDS |
| TCCACCAAGTCCACATATTG+TGG | 0.566013 | 1.3:+32571870 | None:intergenic |
| ATGACAACACCTCTACTACC+AGG | 0.567626 | 1.3:+32572459 | None:intergenic |
| TCAAGAGAGTGAGAGTGGAT+TGG | 0.568198 | 1.3:+32572730 | None:intergenic |
| TCTCATTGTTCAACCAACAC+TGG | 0.569882 | 1.3:-32572510 | MS.gene84044:CDS |
| AATGTGGTTATACTATGTGC+AGG | 0.572229 | 1.3:+32571796 | None:intergenic |
| AAGGACTTGGGGTCTAACAT+AGG | 0.577022 | 1.3:-32572699 | MS.gene84044:CDS |
| GTTAAAGAGGAAGTCAGGGG+AGG | 0.578014 | 1.3:-32571395 | MS.gene84044:CDS |
| TTGTTATTATGGAAGAACAA+AGG | 0.585080 | 1.3:-32572104 | MS.gene84044:CDS |
| GCACTATGATCATTATCATG+AGG | 0.591440 | 1.3:+32572208 | None:intergenic |
| TGCCATTAGGGACACAAATG+TGG | 0.591535 | 1.3:+32571780 | None:intergenic |
| GTTTCCTCTTCCCCAAGTAG+AGG | 0.594548 | 1.3:-32571952 | MS.gene84044:CDS |
| ACTAGTGTAAAGAATTTCCC+TGG | 0.597323 | 1.3:-32572477 | MS.gene84044:CDS |
| GCCACAATATGTGGACTTGG+TGG | 0.598104 | 1.3:-32571871 | MS.gene84044:CDS |
| CACTGCTGTTAGCCTCTTAG+GGG | 0.603640 | 1.3:-32571323 | MS.gene84044:CDS |
| ATGATCTTCACCTCTACTTG+GGG | 0.609933 | 1.3:+32571942 | None:intergenic |
| TTATCATGAGGAACCCTGCA+AGG | 0.614007 | 1.3:+32572220 | None:intergenic |
| CCAATTGTTAAGACAACCCA+AGG | 0.620677 | 1.3:+32572642 | None:intergenic |
| GAGGTGTGATTTCATTGAGT+AGG | 0.632454 | 1.3:+32572664 | None:intergenic |
| TGACAACACCTCTACTACCA+GGG | 0.633964 | 1.3:+32572460 | None:intergenic |
| ATCTAGGTAAAGTTACACAA+GGG | 0.638121 | 1.3:-32571747 | MS.gene84044:CDS |
| AGTGACTAAAGCTCCAGTGT+TGG | 0.645448 | 1.3:+32572497 | None:intergenic |
| AGGGTTAAAGAGGAAGTCAG+GGG | 0.655064 | 1.3:-32571398 | MS.gene84044:CDS |
| TATCTAGGTAAAGTTACACA+AGG | 0.663423 | 1.3:-32571748 | MS.gene84044:CDS |
| CCATAATAACAACAACAAGA+GGG | 0.664165 | 1.3:+32572115 | None:intergenic |
| ACTTGGTGGAACTCTTACTG+TGG | 0.667152 | 1.3:-32571857 | MS.gene84044:CDS |
| TCTAGGTAAAGTTACACAAG+GGG | 0.677668 | 1.3:-32571746 | MS.gene84044:CDS |
| ATTGGTTGTGACTCAGAACA+AGG | 0.680575 | 1.3:-32571203 | MS.gene84044:CDS |
| CCACAGACTCACCAATCTAG+AGG | 0.680979 | 1.3:-32572030 | MS.gene84044:CDS |
| TAATAACAACAACAAGAGGG+AGG | 0.686207 | 1.3:+32572118 | None:intergenic |
| CATATACAAGAAGTTCAGAG+AGG | 0.708025 | 1.3:-32571248 | MS.gene84044:CDS |
| ATTGTTAAGACAACCCAAGG+AGG | 0.744267 | 1.3:+32572645 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | CATAAGTAAAATTAAAGATT+TGG | + | chr1.3:32571646-32571665 | None:intergenic | 15.0% |
| !!! | CAAATCTTTAATTTTACTTA+TGG | - | chr1.3:32571644-32571663 | MS.gene84044:CDS | 15.0% |
| !! | AAGGAAATTATAAAAAGCTT+TGG | + | chr1.3:32571748-32571767 | None:intergenic | 20.0% |
| !! | TAGAAATAATGAAACTGAAT+TGG | - | chr1.3:32572781-32572800 | MS.gene84044:CDS | 20.0% |
| ! | ACTCAACATTGTATAATGTT+GGG | - | chr1.3:32572489-32572508 | MS.gene84044:CDS | 25.0% |
| ! | AGTCAAATTGGATTATCTTT+AGG | - | chr1.3:32572179-32572198 | MS.gene84044:CDS | 25.0% |
| ! | ATGTAGCATCAATTATTGTT+GGG | - | chr1.3:32572399-32572418 | MS.gene84044:CDS | 25.0% |
| ! | ATGTGCTTATACATATTCAT+TGG | - | chr1.3:32571462-32571481 | MS.gene84044:CDS | 25.0% |
| ! | GATATGATTGAAAATAGTAC+TGG | + | chr1.3:32572446-32572465 | None:intergenic | 25.0% |
| ! | GTAAATAACTTGAGTCAAAT+TGG | - | chr1.3:32572167-32572186 | MS.gene84044:CDS | 25.0% |
| ! | TAACAAAGTTAAAACTTCCT+AGG | - | chr1.3:32572297-32572316 | MS.gene84044:CDS | 25.0% |
| ! | TACTCAACATTGTATAATGT+TGG | - | chr1.3:32572488-32572507 | MS.gene84044:CDS | 25.0% |
| ! | TATAAGCACATATTCCATAT+TGG | + | chr1.3:32571453-32571472 | None:intergenic | 25.0% |
| ! | TATGTAGCATCAATTATTGT+TGG | - | chr1.3:32572398-32572417 | MS.gene84044:CDS | 25.0% |
| !! | AAGAGAAATCAAATCAGTTT+TGG | - | chr1.3:32571242-32571261 | MS.gene84044:CDS | 25.0% |
| !! | AGAGAAATCAAATCAGTTTT+GGG | - | chr1.3:32571243-32571262 | MS.gene84044:CDS | 25.0% |
| !! | TTGTTATTATGGAAGAACAA+AGG | - | chr1.3:32571898-32571917 | MS.gene84044:CDS | 25.0% |
| !!! | CTTTAATTTTACTTATGGCT+TGG | - | chr1.3:32571649-32571668 | MS.gene84044:CDS | 25.0% |
| !!! | TAAAAAGCTTTGGAATCATT+TGG | + | chr1.3:32571738-32571757 | None:intergenic | 25.0% |
| !!! | TTTTTGGCTATTTCATGATT+TGG | - | chr1.3:32571397-32571416 | MS.gene84044:CDS | 25.0% |
| AAAACAAAAGTTACAGCAGT+TGG | + | chr1.3:32571678-32571697 | None:intergenic | 30.0% | |
| ATAGATCCATATTGCCATTA+GGG | + | chr1.3:32572237-32572256 | None:intergenic | 30.0% | |
| ATATTCCATATTGGAGGTTT+AGG | + | chr1.3:32571444-32571463 | None:intergenic | 30.0% | |
| ATCTAGGTAAAGTTACACAA+GGG | - | chr1.3:32572255-32572274 | MS.gene84044:CDS | 30.0% | |
| ATGCTACATAAAGACCATTT+GGG | + | chr1.3:32572388-32572407 | None:intergenic | 30.0% | |
| ATTGCATTTAATGTCCCAAA+TGG | - | chr1.3:32572371-32572390 | MS.gene84044:CDS | 30.0% | |
| CAAGGATTAAAGACATGTAA+AGG | + | chr1.3:32571767-32571786 | None:intergenic | 30.0% | |
| CCATAATAACAACAACAAGA+GGG | + | chr1.3:32571890-32571909 | None:intergenic | 30.0% | |
| GGACATTAAATGCAATTAGA+AGG | + | chr1.3:32572367-32572386 | None:intergenic | 30.0% | |
| TATCTAGGTAAAGTTACACA+AGG | - | chr1.3:32572254-32572273 | MS.gene84044:CDS | 30.0% | |
| TATTCCATATTGGAGGTTTA+GGG | + | chr1.3:32571443-32571462 | None:intergenic | 30.0% | |
| TCAATCATATCTGAACTCTT+TGG | - | chr1.3:32572455-32572474 | MS.gene84044:CDS | 30.0% | |
| TCCATAATAACAACAACAAG+AGG | + | chr1.3:32571891-32571910 | None:intergenic | 30.0% | |
| TTATGGAAGAACAAAGGATT+TGG | - | chr1.3:32571904-32571923 | MS.gene84044:CDS | 30.0% | |
| TTTATGATCATGTCAGTATC+TGG | - | chr1.3:32571192-32571211 | MS.gene84044:CDS | 30.0% | |
| ! | GTCATACTTGTTAAGTGTTA+GGG | - | chr1.3:32572532-32572551 | MS.gene84044:CDS | 30.0% |
| !! | AGGACTAGAGAGTTTTATAA+AGG | - | chr1.3:32572728-32572747 | MS.gene84044:CDS | 30.0% |
| AAATCCCTAAACCTCCAATA+TGG | - | chr1.3:32571436-32571455 | MS.gene84044:CDS | 35.0% | |
| AACCAAGATAACCACTCAAA+AGG | + | chr1.3:32571572-32571591 | None:intergenic | 35.0% | |
| AAGCACATATTCCATATTGG+AGG | + | chr1.3:32571450-32571469 | None:intergenic | 35.0% | |
| AATGTGGTTATACTATGTGC+AGG | + | chr1.3:32572209-32572228 | None:intergenic | 35.0% | |
| ACATGTCTTTAATCCTTGCA+GGG | - | chr1.3:32571769-32571788 | MS.gene84044:CDS | 35.0% | |
| ACTAGTGTAAAGAATTTCCC+TGG | - | chr1.3:32571525-32571544 | MS.gene84044:CDS | 35.0% | |
| ATCCTTATCTTGTCTTGTGT+TGG | - | chr1.3:32572338-32572357 | MS.gene84044:CDS | 35.0% | |
| ATGGCAATATGGATCTATCT+AGG | - | chr1.3:32572239-32572258 | MS.gene84044:CDS | 35.0% | |
| ATTATTGTTGGGTTCTGCTT+TGG | - | chr1.3:32572410-32572429 | MS.gene84044:CDS | 35.0% | |
| CAAAAAGAGTCATCATGTCA+AGG | + | chr1.3:32572109-32572128 | None:intergenic | 35.0% | |
| CAACTATACTATGCCTTCTA+TGG | - | chr1.3:32571612-32571631 | MS.gene84044:CDS | 35.0% | |
| CATATACAAGAAGTTCAGAG+AGG | - | chr1.3:32572754-32572773 | MS.gene84044:CDS | 35.0% | |
| CTCTTATGGTGCTTAATAAC+TGG | + | chr1.3:32571705-32571724 | None:intergenic | 35.0% | |
| CTTAACAAGTATGACCCTAT+TGG | + | chr1.3:32572527-32572546 | None:intergenic | 35.0% | |
| GATAGATCCATATTGCCATT+AGG | + | chr1.3:32572238-32572257 | None:intergenic | 35.0% | |
| GATGCTACATAAAGACCATT+TGG | + | chr1.3:32572389-32572408 | None:intergenic | 35.0% | |
| GCACTATGATCATTATCATG+AGG | + | chr1.3:32571797-32571816 | None:intergenic | 35.0% | |
| TAAAGCAAATGGCAGCATTA+GGG | - | chr1.3:32572585-32572604 | MS.gene84044:CDS | 35.0% | |
| TAATAACAACAACAAGAGGG+AGG | + | chr1.3:32571887-32571906 | None:intergenic | 35.0% | |
| TACATGTCTTTAATCCTTGC+AGG | - | chr1.3:32571768-32571787 | MS.gene84044:CDS | 35.0% | |
| TATGATCTTCACCTCTACTT+GGG | + | chr1.3:32572064-32572083 | None:intergenic | 35.0% | |
| TCTAGGTAAAGTTACACAAG+GGG | - | chr1.3:32572256-32572275 | MS.gene84044:CDS | 35.0% | |
| ! | CTTTTTGTAGCCACAATATG+TGG | - | chr1.3:32572122-32572141 | MS.gene84044:CDS | 35.0% |
| ! | GGTCATACTTGTTAAGTGTT+AGG | - | chr1.3:32572531-32572550 | MS.gene84044:CDS | 35.0% |
| ! | TAATGCTGCCATTTGCTTTA+GGG | + | chr1.3:32572585-32572604 | None:intergenic | 35.0% |
| ! | TTTGGAGTCATTTCCATAGA+AGG | + | chr1.3:32571628-32571647 | None:intergenic | 35.0% |
| !! | CCCTCTTGTTGTTGTTATTA+TGG | - | chr1.3:32571887-32571906 | MS.gene84044:CDS | 35.0% |
| !! | TGTCTCTAACTTTGGTGTTA+AGG | - | chr1.3:32572708-32572727 | MS.gene84044:CDS | 35.0% |
| AACCACATTTGTGTCCCTAA+TGG | - | chr1.3:32572220-32572239 | MS.gene84044:CDS | 40.0% | |
| AAGAATTTCCCTGGTAGTAG+AGG | - | chr1.3:32571534-32571553 | MS.gene84044:CDS | 40.0% | |
| ATGATCTTCACCTCTACTTG+GGG | + | chr1.3:32572063-32572082 | None:intergenic | 40.0% | |
| ATTGGTTGTGACTCAGAACA+AGG | - | chr1.3:32572799-32572818 | MS.gene84044:CDS | 40.0% | |
| ATTGTTAAGACAACCCAAGG+AGG | + | chr1.3:32571360-32571379 | None:intergenic | 40.0% | |
| CAAAGAAGCCCTAAAGCAAA+TGG | - | chr1.3:32572574-32572593 | MS.gene84044:CDS | 40.0% | |
| CAATGAAATCACACCTCCTT+GGG | - | chr1.3:32571344-32571363 | MS.gene84044:CDS | 40.0% | |
| CATCATGTCAAGGCTGAAAA+TGG | + | chr1.3:32572099-32572118 | None:intergenic | 40.0% | |
| CCAATTGTTAAGACAACCCA+AGG | + | chr1.3:32571363-32571382 | None:intergenic | 40.0% | |
| CTAAAGCAAATGGCAGCATT+AGG | - | chr1.3:32572584-32572603 | MS.gene84044:CDS | 40.0% | |
| GACCAACACAAGACAAGATA+AGG | + | chr1.3:32572343-32572362 | None:intergenic | 40.0% | |
| GAGGTGTGATTTCATTGAGT+AGG | + | chr1.3:32571341-32571360 | None:intergenic | 40.0% | |
| GGTCAATAGCAAGTCCAATA+GGG | - | chr1.3:32572510-32572529 | MS.gene84044:CDS | 40.0% | |
| GTATGATCTTCACCTCTACT+TGG | + | chr1.3:32572065-32572084 | None:intergenic | 40.0% | |
| TAGGGTTAAAGAGGAAGTCA+GGG | - | chr1.3:32572603-32572622 | MS.gene84044:CDS | 40.0% | |
| TCAATGAAATCACACCTCCT+TGG | - | chr1.3:32571343-32571362 | MS.gene84044:CDS | 40.0% | |
| TCTAACATAGGCATTCTCTC+AGG | - | chr1.3:32571315-32571334 | MS.gene84044:CDS | 40.0% | |
| TCTCATTGTTCAACCAACAC+TGG | - | chr1.3:32571492-32571511 | MS.gene84044:CDS | 40.0% | |
| TGGAAGTCAGTGCTACAAAT+TGG | - | chr1.3:32572643-32572662 | MS.gene84044:CDS | 40.0% | |
| TGGGTTGTCTTAACAATTGG+TGG | - | chr1.3:32571363-32571382 | MS.gene84044:CDS | 40.0% | |
| TTAGGGTTAAAGAGGAAGTC+AGG | - | chr1.3:32572602-32572621 | MS.gene84044:CDS | 40.0% | |
| TTGAGCTTCTTCAAGGACTT+GGG | - | chr1.3:32571291-32571310 | MS.gene84044:CDS | 40.0% | |
| TTGGTTGTTCAGCTCTCTTA+TGG | + | chr1.3:32571719-32571738 | None:intergenic | 40.0% | |
| TTGTGTCCCTAATGGCAATA+TGG | - | chr1.3:32572228-32572247 | MS.gene84044:CDS | 40.0% | |
| TTGTTAAGTGTTAGGGTTGC+TGG | - | chr1.3:32572539-32572558 | MS.gene84044:CDS | 40.0% | |
| ! | AGTGGTTATCTTGGTTTGAG+TGG | - | chr1.3:32571576-32571595 | MS.gene84044:CDS | 40.0% |
| ! | CCTTGGGTTGTCTTAACAAT+TGG | - | chr1.3:32571360-32571379 | MS.gene84044:CDS | 40.0% |
| ! | CTAATGCTGCCATTTGCTTT+AGG | + | chr1.3:32572586-32572605 | None:intergenic | 40.0% |
| !! | GAGCAAGAGAAGCATCAAAA+TGG | + | chr1.3:32571862-32571881 | None:intergenic | 40.0% |
| !!! | GGTGGTCTTCTCAACTTTTT+TGG | - | chr1.3:32571381-32571400 | MS.gene84044:CDS | 40.0% |
| AAGGACTTGGGGTCTAACAT+AGG | - | chr1.3:32571303-32571322 | MS.gene84044:CDS | 45.0% | |
| AGGATTGATGTGAGCATGAG+AGG | + | chr1.3:32572323-32572342 | None:intergenic | 45.0% | |
| AGGGTTAAAGAGGAAGTCAG+GGG | - | chr1.3:32572604-32572623 | MS.gene84044:CDS | 45.0% | |
| AGTGACTAAAGCTCCAGTGT+TGG | + | chr1.3:32571508-32571527 | None:intergenic | 45.0% | |
| ATGACAACACCTCTACTACC+AGG | + | chr1.3:32571546-32571565 | None:intergenic | 45.0% | |
| CACTCTCTTGAGCTTCTTCA+AGG | - | chr1.3:32571284-32571303 | MS.gene84044:CDS | 45.0% | |
| CTTGAGCTTCTTCAAGGACT+TGG | - | chr1.3:32571290-32571309 | MS.gene84044:CDS | 45.0% | |
| GAGTAGTCTCTCCTCTAGAT+TGG | + | chr1.3:32571986-32572005 | None:intergenic | 45.0% | |
| GGCACTTGTGTCTCTAACTT+TGG | - | chr1.3:32572700-32572719 | MS.gene84044:CDS | 45.0% | |
| GGGTCAATAGCAAGTCCAAT+AGG | - | chr1.3:32572509-32572528 | MS.gene84044:CDS | 45.0% | |
| GTAGCCACAATATGTGGACT+TGG | - | chr1.3:32572128-32572147 | MS.gene84044:CDS | 45.0% | |
| GTTGTGACTCAGAACAAGGT+TGG | - | chr1.3:32572803-32572822 | MS.gene84044:CDS | 45.0% | |
| TCAAGAGAGTGAGAGTGGAT+TGG | + | chr1.3:32571275-32571294 | None:intergenic | 45.0% | |
| TCCACCAAGTCCACATATTG+TGG | + | chr1.3:32572135-32572154 | None:intergenic | 45.0% | |
| TGACAACACCTCTACTACCA+GGG | + | chr1.3:32571545-32571564 | None:intergenic | 45.0% | |
| TGAGCTTCTTCAAGGACTTG+GGG | - | chr1.3:32571292-32571311 | MS.gene84044:CDS | 45.0% | |
| TGCCATTAGGGACACAAATG+TGG | + | chr1.3:32572225-32572244 | None:intergenic | 45.0% | |
| TGGCAGCATTAGGGTTAAAG+AGG | - | chr1.3:32572594-32572613 | MS.gene84044:CDS | 45.0% | |
| TTATCATGAGGAACCCTGCA+AGG | + | chr1.3:32571785-32571804 | None:intergenic | 45.0% | |
| ! | ACTTGGTGGAACTCTTACTG+TGG | - | chr1.3:32572145-32572164 | MS.gene84044:CDS | 45.0% |
| ! | ATCACTGCTGTTAGCCTCTT+AGG | - | chr1.3:32572677-32572696 | MS.gene84044:CDS | 45.0% |
| ! | GGTTCTGCTTTGGTGCAAAT+TGG | - | chr1.3:32572420-32572439 | MS.gene84044:CDS | 45.0% |
| ! | GTTGTCATAGGCCTTTTGAG+TGG | - | chr1.3:32571558-32571577 | MS.gene84044:CDS | 45.0% |
| ! | TCACTGCTGTTAGCCTCTTA+GGG | - | chr1.3:32572678-32572697 | MS.gene84044:CDS | 45.0% |
| ! | TCTGGAGCAAGCTACATGTT+TGG | - | chr1.3:32571210-32571229 | MS.gene84044:CDS | 45.0% |
| !! | GGCCTTTTGAGTGGTTATCT+TGG | - | chr1.3:32571567-32571586 | MS.gene84044:CDS | 45.0% |
| !! | GGTAGTAGAGGTGTTGTCAT+AGG | - | chr1.3:32571546-32571565 | MS.gene84044:CDS | 45.0% |
| CCACAGACTCACCAATCTAG+AGG | - | chr1.3:32571972-32571991 | MS.gene84044:CDS | 50.0% | |
| CCTCTAGATTGGTGAGTCTG+TGG | + | chr1.3:32571975-32571994 | None:intergenic | 50.0% | |
| GAAGCTCAAGAGAGTGAGAG+TGG | + | chr1.3:32571280-32571299 | None:intergenic | 50.0% | |
| GATTGGTGAGTCTGTGGCTT+AGG | + | chr1.3:32571969-32571988 | None:intergenic | 50.0% | |
| GCCACAATATGTGGACTTGG+TGG | - | chr1.3:32572131-32572150 | MS.gene84044:CDS | 50.0% | |
| GGGGAGGAGTTGAATTGCAA+TGG | - | chr1.3:32572623-32572642 | MS.gene84044:CDS | 50.0% | |
| GTTAAAGAGGAAGTCAGGGG+AGG | - | chr1.3:32572607-32572626 | MS.gene84044:CDS | 50.0% | |
| GTTTCCTCTTCCCCAAGTAG+AGG | - | chr1.3:32572050-32572069 | MS.gene84044:CDS | 50.0% | |
| TTCACCTCTACTTGGGGAAG+AGG | + | chr1.3:32572057-32572076 | None:intergenic | 50.0% | |
| ! | CACTGCTGTTAGCCTCTTAG+GGG | - | chr1.3:32572679-32572698 | MS.gene84044:CDS | 50.0% |
| GAGACACAAGTGCCCCTAAG+AGG | + | chr1.3:32572694-32572713 | None:intergenic | 55.0% | |
| GATGTGAGCATGAGAGGCCT+AGG | + | chr1.3:32572317-32572336 | None:intergenic | 55.0% | |
| TGAGTCTGTGGCTTAGGAGG+AGG | + | chr1.3:32571963-32571982 | None:intergenic | 55.0% | |
| TGGTGAGTCTGTGGCTTAGG+AGG | + | chr1.3:32571966-32571985 | None:intergenic | 55.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr1.3 | gene | 32571177 | 32572847 | 32571177 | ID=MS.gene84044 |
| chr1.3 | mRNA | 32571177 | 32572847 | 32571177 | ID=MS.gene84044.t1;Parent=MS.gene84044 |
| chr1.3 | exon | 32571177 | 32572847 | 32571177 | ID=MS.gene84044.t1.exon1;Parent=MS.gene84044.t1 |
| chr1.3 | CDS | 32571177 | 32572847 | 32571177 | ID=cds.MS.gene84044.t1;Parent=MS.gene84044.t1 |
| Gene Sequence |
| Protein sequence |
