Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene84433.t1 | GAU13365.1 | 98 | 249 | 5 | 0 | 1 | 249 | 1 | 249 | 1.40E-132 | 482.3 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene84433.t1 | Q9FJK7 | 75.1 | 249 | 62 | 0 | 1 | 249 | 1 | 249 | 7.1e-109 | 394.8 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene84433.t1 | A0A2Z6LL52 | 98.0 | 249 | 5 | 0 | 1 | 249 | 1 | 249 | 1.0e-132 | 482.3 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI:
| Gene1 | Gene2 | Type |
|---|---|---|
| MS.gene84433 | MS.gene028061 | PPI |
| MS.gene84433 | MS.gene83422 | PPI |
| MS.gene82967 | MS.gene84433 | PPI |
| MS.gene84433 | MS.gene28739 | PPI |
| MS.gene35086 | MS.gene84433 | PPI |
| MS.gene84433 | MS.gene001116 | PPI |
| MS.gene84433 | MS.gene36424 | PPI |
| MS.gene84433 | MS.gene056537 | PPI |
| MS.gene84433 | MS.gene028366 | PPI |
| MS.gene84433 | MS.gene067895 | PPI |
| MS.gene84433 | MS.gene30693 | PPI |
| MS.gene84433 | MS.gene72846 | PPI |
| MS.gene84433 | MS.gene049236 | PPI |
| MS.gene84433 | MS.gene55900 | PPI |
| MS.gene84433 | MS.gene86051 | PPI |
| MS.gene84433 | MS.gene57169 | PPI |
| MS.gene84433 | MS.gene031102 | PPI |
| MS.gene84433 | MS.gene070097 | PPI |
| MS.gene84433 | MS.gene00063 | PPI |
| MS.gene84433 | MS.gene013265 | PPI |
| MS.gene84433 | MS.gene06350 | PPI |
| MS.gene84433 | MS.gene071476 | PPI |
| MS.gene67884 | MS.gene84433 | PPI |
| MS.gene84433 | MS.gene40939 | PPI |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene84433.t1 | MTR_7g055650 | 96.386 | 249 | 9 | 0 | 1 | 249 | 1 | 249 | 1.58e-180 | 495 |
| MS.gene84433.t1 | MTR_7g055650 | 96.347 | 219 | 8 | 0 | 1 | 219 | 1 | 219 | 3.87e-157 | 437 |
| MS.gene84433.t1 | MTR_2g068690 | 26.522 | 230 | 152 | 3 | 24 | 236 | 43 | 272 | 5.21e-22 | 95.1 |
| MS.gene84433.t1 | MTR_4g083690 | 26.522 | 230 | 152 | 4 | 24 | 236 | 44 | 273 | 8.63e-21 | 91.3 |
| MS.gene84433.t1 | MTR_7g094070 | 24.336 | 226 | 154 | 2 | 24 | 232 | 14 | 239 | 1.83e-17 | 81.3 |
| MS.gene84433.t1 | MTR_1g104820 | 23.605 | 233 | 162 | 4 | 14 | 231 | 141 | 372 | 8.55e-14 | 70.5 |
| MS.gene84433.t1 | MTR_1g104820 | 23.605 | 233 | 162 | 4 | 14 | 231 | 202 | 433 | 1.41e-13 | 70.1 |
| MS.gene84433.t1 | MTR_1g083060 | 27.322 | 183 | 114 | 5 | 30 | 202 | 71 | 244 | 2.54e-11 | 63.2 |
| MS.gene84433.t1 | MTR_5g010140 | 24.103 | 195 | 132 | 4 | 19 | 199 | 13 | 205 | 7.45e-11 | 62.0 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene84433.t1 | AT5G48630 | 75.100 | 249 | 62 | 0 | 1 | 249 | 1 | 249 | 2.94e-148 | 414 |
| MS.gene84433.t1 | AT5G48630 | 74.583 | 240 | 61 | 0 | 10 | 249 | 13 | 252 | 6.84e-140 | 393 |
| MS.gene84433.t1 | AT5G48640 | 73.494 | 249 | 66 | 0 | 1 | 249 | 1 | 249 | 1.92e-139 | 392 |
| MS.gene84433.t1 | AT5G48640 | 74.627 | 201 | 51 | 0 | 49 | 249 | 1 | 201 | 9.12e-111 | 317 |
| MS.gene84433.t1 | AT5G48640 | 74.033 | 181 | 47 | 0 | 69 | 249 | 1 | 181 | 1.34e-97 | 283 |
| MS.gene84433.t1 | AT5G48640 | 74.033 | 181 | 47 | 0 | 69 | 249 | 1 | 181 | 1.34e-97 | 283 |
| MS.gene84433.t1 | AT5G48640 | 74.033 | 181 | 47 | 0 | 69 | 249 | 1 | 181 | 1.34e-97 | 283 |
| MS.gene84433.t1 | AT5G45190 | 26.457 | 223 | 147 | 2 | 31 | 236 | 50 | 272 | 1.77e-22 | 96.3 |
| MS.gene84433.t1 | AT4G19600 | 27.536 | 207 | 133 | 3 | 47 | 236 | 66 | 272 | 1.61e-21 | 93.6 |
| MS.gene84433.t1 | AT5G45190 | 27.461 | 193 | 123 | 2 | 61 | 236 | 91 | 283 | 1.70e-20 | 90.5 |
| MS.gene84433.t1 | AT1G35440 | 25.110 | 227 | 157 | 4 | 17 | 231 | 11 | 236 | 4.07e-17 | 78.6 |
| MS.gene84433.t1 | AT1G27630 | 23.750 | 240 | 158 | 7 | 12 | 232 | 33 | 266 | 3.39e-13 | 68.6 |
| MS.gene84433.t1 | AT4G19560 | 26.339 | 224 | 150 | 5 | 24 | 232 | 41 | 264 | 4.29e-13 | 68.6 |
| MS.gene84433.t1 | AT1G27630 | 23.605 | 233 | 153 | 7 | 12 | 225 | 33 | 259 | 1.23e-12 | 66.6 |
| MS.gene84433.t1 | AT5G45190 | 26.380 | 163 | 103 | 2 | 91 | 236 | 1 | 163 | 1.93e-12 | 66.6 |
| MS.gene84433.t1 | AT2G26430 | 25.258 | 194 | 126 | 6 | 55 | 231 | 49 | 240 | 4.87e-11 | 62.0 |
| MS.gene84433.t1 | AT2G26430 | 25.258 | 194 | 126 | 6 | 55 | 231 | 49 | 240 | 7.91e-11 | 62.0 |
Find 42 sgRNAs with CRISPR-Local
Find 0 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| CACTCATGGCTGCCAATTTC+TGG | 0.123595 | 7.4:+49099058 | None:intergenic |
| ATCTTGCAGCAAGCATCTTT+TGG | 0.291299 | 7.4:+49099168 | MS.gene84433:intron |
| ATAATTCTCCAGGTTGCTTC+AGG | 0.321062 | 7.4:+49102991 | MS.gene84433:intron |
| ATGAGATGATGTCCAGAAAT+TGG | 0.334274 | 7.4:-49099070 | None:intergenic |
| CACCCATACCGTTCCCTCTC+CGG | 0.359671 | 7.4:+49101568 | MS.gene84433:CDS |
| AATCAAGGACATACTTGAAA+TGG | 0.375937 | 7.4:+49101501 | MS.gene84433:CDS |
| TCCAGGTTGCTTCAGGATGC+AGG | 0.404579 | 7.4:+49102998 | MS.gene84433:intron |
| AGCTTGCACTGTGCTTTCTT+CGG | 0.414038 | 7.4:-49101281 | None:intergenic |
| GGGATCAAGATCATAGCTTC+TGG | 0.418867 | 7.4:-49108745 | None:intergenic |
| GGATCAAGAAGATGTGGATA+TGG | 0.421298 | 7.4:+49099189 | MS.gene84433:CDS |
| GAATCCAATTGATAAAGAAA+AGG | 0.453468 | 7.4:+49099213 | MS.gene84433:CDS |
| GTGGTGGATGTACCAGCATT+AGG | 0.460425 | 7.4:-49105858 | None:intergenic |
| ACCCATACCGTTCCCTCTCC+GGG | 0.487323 | 7.4:+49101569 | MS.gene84433:CDS |
| AATCCAATTGATAAAGAAAA+GGG | 0.500318 | 7.4:+49099214 | MS.gene84433:CDS |
| TAAGCATGACGCAACTAACT+TGG | 0.507825 | 7.4:+49103032 | MS.gene84433:CDS |
| TATACATTGCTAGTGTACTC+AGG | 0.513103 | 7.4:+49105902 | MS.gene84433:CDS |
| AAGAAAGCACAGTGCAAGCT+AGG | 0.516188 | 7.4:+49101284 | MS.gene84433:CDS |
| GATGCAAGGTACAAGCATGC+TGG | 0.517574 | 7.4:-49101253 | None:intergenic |
| GTCCTTACCCGGAGAGGGAA+CGG | 0.521483 | 7.4:-49101576 | None:intergenic |
| ACCCGGAGAGGGAACGGTAT+GGG | 0.521936 | 7.4:-49101570 | None:intergenic |
| GGGAGAAAGATACCACTGTC+TGG | 0.540279 | 7.4:+49105923 | MS.gene84433:CDS |
| TAAGTATAGATACGAAATCA+AGG | 0.548919 | 7.4:+49101486 | MS.gene84433:CDS |
| AGAAGCATTGAACTATTACC+TGG | 0.558055 | 7.4:+49101537 | MS.gene84433:CDS |
| TACCCGGAGAGGGAACGGTA+TGG | 0.560117 | 7.4:-49101571 | None:intergenic |
| TGTAAATGACACATACAAGA+TGG | 0.560228 | 7.4:+49105834 | MS.gene84433:CDS |
| AACAAGTAAAAGTGAGGCAA+AGG | 0.561915 | 7.4:+49100058 | MS.gene84433:CDS |
| CGGAGAGGGAACGGTATGGG+TGG | 0.568160 | 7.4:-49101567 | None:intergenic |
| ACAAGCATGCTGGAGCTACA+AGG | 0.571931 | 7.4:-49101243 | None:intergenic |
| AGAAAAGGGTGTCACACTTG+AGG | 0.584109 | 7.4:+49099228 | MS.gene84433:CDS |
| CATGCTGGAGCTACAAGGCG+TGG | 0.589669 | 7.4:-49101238 | None:intergenic |
| AATTTGTCCTTACCCGGAGA+GGG | 0.590503 | 7.4:-49101581 | None:intergenic |
| CAATTTGTCCTTACCCGGAG+AGG | 0.594658 | 7.4:-49101582 | None:intergenic |
| ATACATTGCTAGTGTACTCA+GGG | 0.596843 | 7.4:+49105903 | MS.gene84433:CDS |
| GCTAAAGCAATCAGATGTGG+TGG | 0.599634 | 7.4:-49105874 | None:intergenic |
| ATACAAGATGGACCTAATGC+TGG | 0.604425 | 7.4:+49105846 | MS.gene84433:CDS |
| TGGCTCAACAAGTAAAAGTG+AGG | 0.644104 | 7.4:+49100052 | MS.gene84433:CDS |
| CAAGCTAAAGCAATCAGATG+TGG | 0.656410 | 7.4:-49105877 | None:intergenic |
| ACATGAGGCGTGTTTACACA+AGG | 0.673715 | 7.4:+49100707 | MS.gene84433:CDS |
| CTACTGCAGTTACATACATG+AGG | 0.681090 | 7.4:+49100692 | MS.gene84433:CDS |
| GGTATGGGTGGAATACAACC+AGG | 0.688220 | 7.4:-49101555 | None:intergenic |
| ACTTCGTGTTGATATGAATG+TGG | 0.711326 | 7.4:+49105954 | MS.gene84433:CDS |
| AAGTTCTTCAAACCAGACAG+TGG | 0.712400 | 7.4:-49105935 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr7.4 | gene | 49099063 | 49108757 | 49099063 | ID=MS.gene84433 |
| chr7.4 | mRNA | 49099063 | 49108757 | 49099063 | ID=MS.gene84433.t1;Parent=MS.gene84433 |
| chr7.4 | exon | 49099063 | 49099094 | 49099063 | ID=MS.gene84433.t1.exon1;Parent=MS.gene84433.t1 |
| chr7.4 | CDS | 49099063 | 49099094 | 49099063 | ID=cds.MS.gene84433.t1;Parent=MS.gene84433.t1 |
| chr7.4 | exon | 49099177 | 49099283 | 49099177 | ID=MS.gene84433.t1.exon2;Parent=MS.gene84433.t1 |
| chr7.4 | CDS | 49099177 | 49099283 | 49099177 | ID=cds.MS.gene84433.t1;Parent=MS.gene84433.t1 |
| chr7.4 | exon | 49100040 | 49100079 | 49100040 | ID=MS.gene84433.t1.exon3;Parent=MS.gene84433.t1 |
| chr7.4 | CDS | 49100040 | 49100079 | 49100040 | ID=cds.MS.gene84433.t1;Parent=MS.gene84433.t1 |
| chr7.4 | exon | 49100684 | 49100728 | 49100684 | ID=MS.gene84433.t1.exon4;Parent=MS.gene84433.t1 |
| chr7.4 | CDS | 49100684 | 49100728 | 49100684 | ID=cds.MS.gene84433.t1;Parent=MS.gene84433.t1 |
| chr7.4 | exon | 49101216 | 49101334 | 49101216 | ID=MS.gene84433.t1.exon5;Parent=MS.gene84433.t1 |
| chr7.4 | CDS | 49101216 | 49101334 | 49101216 | ID=cds.MS.gene84433.t1;Parent=MS.gene84433.t1 |
| chr7.4 | exon | 49101476 | 49101590 | 49101476 | ID=MS.gene84433.t1.exon6;Parent=MS.gene84433.t1 |
| chr7.4 | CDS | 49101476 | 49101590 | 49101476 | ID=cds.MS.gene84433.t1;Parent=MS.gene84433.t1 |
| chr7.4 | exon | 49103003 | 49103053 | 49103003 | ID=MS.gene84433.t1.exon7;Parent=MS.gene84433.t1 |
| chr7.4 | CDS | 49103003 | 49103053 | 49103003 | ID=cds.MS.gene84433.t1;Parent=MS.gene84433.t1 |
| chr7.4 | exon | 49105828 | 49105975 | 49105828 | ID=MS.gene84433.t1.exon8;Parent=MS.gene84433.t1 |
| chr7.4 | CDS | 49105828 | 49105975 | 49105828 | ID=cds.MS.gene84433.t1;Parent=MS.gene84433.t1 |
| chr7.4 | exon | 49108665 | 49108757 | 49108665 | ID=MS.gene84433.t1.exon9;Parent=MS.gene84433.t1 |
| chr7.4 | CDS | 49108665 | 49108757 | 49108665 | ID=cds.MS.gene84433.t1;Parent=MS.gene84433.t1 |
| Gene Sequence |
| Protein sequence |
