Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene84452.t1 | PNX93291.1 | 88.9 | 217 | 24 | 0 | 1 | 217 | 1 | 217 | 4.40E-98 | 367.5 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene84452.t1 | P53163 | 36.5 | 167 | 96 | 4 | 61 | 217 | 28 | 194 | 2.1e-16 | 87.4 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene84452.t1 | A0A2K3MR37 | 88.9 | 217 | 24 | 0 | 1 | 217 | 1 | 217 | 3.2e-98 | 367.5 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI:
| Gene1 | Gene2 | Type |
|---|---|---|
| MS.gene005792 | MS.gene84452 | PPI |
| MS.gene84452 | MS.gene09988 | PPI |
| MS.gene60517 | MS.gene84452 | PPI |
| MS.gene68085 | MS.gene84452 | PPI |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene84452.t1 | MTR_7g053520 | 95.392 | 217 | 10 | 0 | 1 | 217 | 1 | 217 | 4.37e-143 | 398 |
| MS.gene84452.t1 | MTR_2g064300 | 86.636 | 217 | 28 | 1 | 1 | 217 | 1 | 216 | 1.68e-125 | 353 |
| MS.gene84452.t1 | MTR_2g031760 | 41.212 | 165 | 92 | 3 | 54 | 217 | 30 | 190 | 1.89e-35 | 124 |
| MS.gene84452.t1 | MTR_8g076780 | 47.368 | 152 | 70 | 2 | 75 | 217 | 45 | 195 | 1.14e-31 | 114 |
| MS.gene84452.t1 | MTR_2g031960 | 48.872 | 133 | 63 | 2 | 86 | 217 | 27 | 155 | 2.86e-30 | 109 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene84452.t1 | AT1G70190 | 55.656 | 221 | 81 | 4 | 1 | 217 | 1 | 208 | 1.41e-67 | 206 |
| MS.gene84452.t1 | AT1G70190 | 55.656 | 221 | 81 | 4 | 1 | 217 | 1 | 208 | 1.41e-67 | 206 |
| MS.gene84452.t1 | AT4G36420 | 42.932 | 191 | 92 | 5 | 31 | 217 | 2 | 179 | 3.05e-34 | 120 |
| MS.gene84452.t1 | AT3G06040 | 45.333 | 150 | 73 | 3 | 69 | 217 | 45 | 186 | 5.26e-32 | 115 |
| MS.gene84452.t1 | AT3G06040 | 45.333 | 150 | 73 | 3 | 69 | 217 | 45 | 186 | 5.26e-32 | 115 |
| MS.gene84452.t1 | AT3G06040 | 45.333 | 150 | 73 | 3 | 69 | 217 | 45 | 186 | 5.26e-32 | 115 |
| MS.gene84452.t1 | AT4G37660 | 70.588 | 68 | 20 | 0 | 150 | 217 | 100 | 167 | 2.91e-25 | 97.4 |
| MS.gene84452.t1 | AT2G03130 | 59.494 | 79 | 32 | 0 | 139 | 217 | 13 | 91 | 1.28e-15 | 70.5 |
Find 47 sgRNAs with CRISPR-Local
Find 59 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| CTCCTTGATGACCTTAATTT+TGG | 0.127344 | 7.4:-49603193 | None:intergenic |
| GAAACCAGGATCTGTAAATT+TGG | 0.208162 | 7.4:+49603074 | MS.gene84452:CDS |
| ACGCCTTTCTTTATAATAGA+AGG | 0.283177 | 7.4:-49603273 | None:intergenic |
| TGAAGCAGCTTCCAAAATTA+AGG | 0.341436 | 7.4:+49603182 | MS.gene84452:CDS |
| TCTAGCTGAAGGTGCAGAAT+TGG | 0.346308 | 7.4:+49602996 | MS.gene84452:CDS |
| TTGTCTATACAACAAACTAT+TGG | 0.364579 | 7.4:-49602746 | None:intergenic |
| CCTCTTACTGCTAGCACTCT+AGG | 0.374115 | 7.4:+49602769 | MS.gene84452:CDS |
| CCAGCCAAATTTACAGATCC+TGG | 0.386608 | 7.4:-49603078 | None:intergenic |
| CGATGGAGGCCCACGATGAT+TGG | 0.408595 | 7.4:-49602932 | None:intergenic |
| TCTAGGCGCAATTGTTGTTA+TGG | 0.415605 | 7.4:+49603338 | MS.gene84452:CDS |
| GAAGGAGGCAAAGGATTTAG+TGG | 0.417133 | 7.4:+49603242 | MS.gene84452:CDS |
| TGTCTATACAACAAACTATT+GGG | 0.417171 | 7.4:-49602745 | None:intergenic |
| CCAGGATCTGTAAATTTGGC+TGG | 0.453905 | 7.4:+49603078 | MS.gene84452:CDS |
| CTAGCTGAAGGTGCAGAATT+GGG | 0.458679 | 7.4:+49602997 | MS.gene84452:CDS |
| CCTAGAGTGCTAGCAGTAAG+AGG | 0.460957 | 7.4:-49602769 | None:intergenic |
| ATCTGTAAATTTGGCTGGAA+TGG | 0.461697 | 7.4:+49603083 | MS.gene84452:CDS |
| CTTTCCTTGCAGCTTGACCT+AGG | 0.475135 | 7.4:-49602828 | None:intergenic |
| TTGGTAGGATCAAATGTTGC+AGG | 0.479265 | 7.4:-49602913 | None:intergenic |
| GGTTTCATAAATCCCACATT+GGG | 0.482477 | 7.4:-49603057 | None:intergenic |
| TATAAAGAAAGGCGTTTCAA+AGG | 0.499632 | 7.4:+49603281 | MS.gene84452:CDS |
| ACACCTTCTATTATAAAGAA+AGG | 0.505048 | 7.4:+49603270 | MS.gene84452:CDS |
| GTTGCAGGATCAAAATCAGT+TGG | 0.513514 | 7.4:-49602898 | None:intergenic |
| CTGAAAACTCTTTCCGATGG+AGG | 0.526100 | 7.4:-49602946 | None:intergenic |
| TTCCAAAATTAAGGTCATCA+AGG | 0.526956 | 7.4:+49603191 | MS.gene84452:CDS |
| TTTCAGGCTTGTTGATGAAG+TGG | 0.533852 | 7.4:+49602963 | MS.gene84452:CDS |
| CAATCATCGTGGGCCTCCAT+CGG | 0.534976 | 7.4:+49602933 | MS.gene84452:CDS |
| AAGGTCATCAAGGAGGTTCG+AGG | 0.535857 | 7.4:+49603201 | MS.gene84452:CDS |
| CTCTTACTGCTAGCACTCTA+GGG | 0.540669 | 7.4:+49602770 | MS.gene84452:CDS |
| ATAGAGAAATTGAAAGCTCT+AGG | 0.542277 | 7.4:+49603321 | MS.gene84452:CDS |
| TTTAGGTTTGAAGGAGGCAA+AGG | 0.553743 | 7.4:+49603233 | MS.gene84452:CDS |
| AATGTATTTACAAGAAACCT+AGG | 0.555772 | 7.4:+49602811 | MS.gene84452:CDS |
| TTGCAGGATCAAAATCAGTT+GGG | 0.555773 | 7.4:-49602897 | None:intergenic |
| AAAGGCGTTTCAAAGGAAGA+AGG | 0.561877 | 7.4:+49603288 | MS.gene84452:CDS |
| AGCTGCAAGGAAAGATGAAG+AGG | 0.574214 | 7.4:+49602837 | MS.gene84452:CDS |
| AGCCTGAAAACTCTTTCCGA+TGG | 0.583898 | 7.4:-49602949 | None:intergenic |
| GGAGGCCCACGATGATTGGT+AGG | 0.590246 | 7.4:-49602928 | None:intergenic |
| TGGTTTCATAAATCCCACAT+TGG | 0.595026 | 7.4:-49603058 | None:intergenic |
| TACTGATTTAGGTTTGAAGG+AGG | 0.609454 | 7.4:+49603227 | MS.gene84452:CDS |
| GAAACCTAGGTCAAGCTGCA+AGG | 0.610947 | 7.4:+49602824 | MS.gene84452:CDS |
| CAAAATTAAGGTCATCAAGG+AGG | 0.615684 | 7.4:+49603194 | MS.gene84452:CDS |
| GCATCTCTAACTCTAGCTGA+AGG | 0.625143 | 7.4:+49602985 | MS.gene84452:CDS |
| AATGTGGGATTTATGAAACC+AGG | 0.639785 | 7.4:+49603060 | MS.gene84452:CDS |
| GATAAAGGAGATGCCCAATG+TGG | 0.647613 | 7.4:+49603044 | MS.gene84452:CDS |
| AACATCAACAGAAGCCAAGG+AGG | 0.649027 | 7.4:+49603113 | MS.gene84452:CDS |
| ATAAAGGAGATGCCCAATGT+GGG | 0.651305 | 7.4:+49603045 | MS.gene84452:CDS |
| TTGATCCTACCAATCATCGT+GGG | 0.673895 | 7.4:+49602923 | MS.gene84452:CDS |
| TTTGATCCTACCAATCATCG+TGG | 0.688414 | 7.4:+49602922 | MS.gene84452:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !!! | AATTCAAATACAGTTTTTTC+TGG | - | chr7.4:49603147-49603166 | None:intergenic | 20.0% |
| ! | AATGTATTTACAAGAAACCT+AGG | + | chr7.4:49602811-49602830 | MS.gene84452:CDS | 25.0% |
| ! | ACACCTTCTATTATAAAGAA+AGG | + | chr7.4:49603270-49603289 | MS.gene84452:CDS | 25.0% |
| ! | TGTCTATACAACAAACTATT+GGG | - | chr7.4:49602748-49602767 | None:intergenic | 25.0% |
| ! | TTGTCTATACAACAAACTAT+TGG | - | chr7.4:49602749-49602768 | None:intergenic | 25.0% |
| !!! | TTTTACTGATTTAGGTTTGA+AGG | + | chr7.4:49603224-49603243 | MS.gene84452:CDS | 25.0% |
| ACGCCTTTCTTTATAATAGA+AGG | - | chr7.4:49603276-49603295 | None:intergenic | 30.0% | |
| ATAGAGAAATTGAAAGCTCT+AGG | + | chr7.4:49603321-49603340 | MS.gene84452:CDS | 30.0% | |
| GATGAAAAAAATGGGGATAA+AGG | + | chr7.4:49603029-49603048 | MS.gene84452:CDS | 30.0% | |
| TTCCAAAATTAAGGTCATCA+AGG | + | chr7.4:49603191-49603210 | MS.gene84452:CDS | 30.0% | |
| !! | TATAAAGAAAGGCGTTTCAA+AGG | + | chr7.4:49603281-49603300 | MS.gene84452:CDS | 30.0% |
| !! | TGAGCTTGCTTTTAAGAATA+AGG | + | chr7.4:49602713-49602732 | MS.gene84452:CDS | 30.0% |
| AAAAACATCAACAGAAGCCA+AGG | + | chr7.4:49603110-49603129 | MS.gene84452:CDS | 35.0% | |
| AATGTGGGATTTATGAAACC+AGG | + | chr7.4:49603060-49603079 | MS.gene84452:CDS | 35.0% | |
| ATCTGTAAATTTGGCTGGAA+TGG | + | chr7.4:49603083-49603102 | MS.gene84452:CDS | 35.0% | |
| CAAAATTAAGGTCATCAAGG+AGG | + | chr7.4:49603194-49603213 | MS.gene84452:CDS | 35.0% | |
| CTCGCTCTGATGAAAAAAAT+GGG | + | chr7.4:49603021-49603040 | MS.gene84452:CDS | 35.0% | |
| GAAACCAGGATCTGTAAATT+TGG | + | chr7.4:49603074-49603093 | MS.gene84452:CDS | 35.0% | |
| GGTTTCATAAATCCCACATT+GGG | - | chr7.4:49603060-49603079 | None:intergenic | 35.0% | |
| TCGCTCTGATGAAAAAAATG+GGG | + | chr7.4:49603022-49603041 | MS.gene84452:CDS | 35.0% | |
| TCTCGCTCTGATGAAAAAAA+TGG | + | chr7.4:49603020-49603039 | MS.gene84452:CDS | 35.0% | |
| TGAAGCAGCTTCCAAAATTA+AGG | + | chr7.4:49603182-49603201 | MS.gene84452:CDS | 35.0% | |
| TGGTTTCATAAATCCCACAT+TGG | - | chr7.4:49603061-49603080 | None:intergenic | 35.0% | |
| TTGCAGGATCAAAATCAGTT+GGG | - | chr7.4:49602900-49602919 | None:intergenic | 35.0% | |
| !! | AATTTTGGAAGCTGCTTCAT+AGG | - | chr7.4:49603181-49603200 | None:intergenic | 35.0% |
| !! | CTCCTTGATGACCTTAATTT+TGG | - | chr7.4:49603196-49603215 | None:intergenic | 35.0% |
| !! | TACTGATTTAGGTTTGAAGG+AGG | + | chr7.4:49603227-49603246 | MS.gene84452:CDS | 35.0% |
| !!! | GTTCGAGGTTTTACTGATTT+AGG | + | chr7.4:49603216-49603235 | MS.gene84452:CDS | 35.0% |
| ATAAAGGAGATGCCCAATGT+GGG | + | chr7.4:49603045-49603064 | MS.gene84452:CDS | 40.0% | |
| GTTGCAGGATCAAAATCAGT+TGG | - | chr7.4:49602901-49602920 | None:intergenic | 40.0% | |
| TTGATCCTACCAATCATCGT+GGG | + | chr7.4:49602923-49602942 | MS.gene84452:CDS | 40.0% | |
| TTGGTAGGATCAAATGTTGC+AGG | - | chr7.4:49602916-49602935 | None:intergenic | 40.0% | |
| TTTGATCCTACCAATCATCG+TGG | + | chr7.4:49602922-49602941 | MS.gene84452:CDS | 40.0% | |
| ! | TCTAGGCGCAATTGTTGTTA+TGG | + | chr7.4:49603338-49603357 | MS.gene84452:CDS | 40.0% |
| ! | TTTAGGTTTGAAGGAGGCAA+AGG | + | chr7.4:49603233-49603252 | MS.gene84452:CDS | 40.0% |
| !! | AAAGGCGTTTCAAAGGAAGA+AGG | + | chr7.4:49603288-49603307 | MS.gene84452:CDS | 40.0% |
| !! | TTTCAGGCTTGTTGATGAAG+TGG | + | chr7.4:49602963-49602982 | MS.gene84452:CDS | 40.0% |
| AACATCAACAGAAGCCAAGG+AGG | + | chr7.4:49603113-49603132 | MS.gene84452:CDS | 45.0% | |
| AGCCTGAAAACTCTTTCCGA+TGG | - | chr7.4:49602952-49602971 | None:intergenic | 45.0% | |
| AGCTGCAAGGAAAGATGAAG+AGG | + | chr7.4:49602837-49602856 | MS.gene84452:CDS | 45.0% | |
| CCAGCCAAATTTACAGATCC+TGG | - | chr7.4:49603081-49603100 | None:intergenic | 45.0% | |
| CCAGGATCTGTAAATTTGGC+TGG | + | chr7.4:49603078-49603097 | MS.gene84452:CDS | 45.0% | |
| CTCTTACTGCTAGCACTCTA+GGG | + | chr7.4:49602770-49602789 | MS.gene84452:CDS | 45.0% | |
| CTGAAAACTCTTTCCGATGG+AGG | - | chr7.4:49602949-49602968 | None:intergenic | 45.0% | |
| GAAGGAGGCAAAGGATTTAG+TGG | + | chr7.4:49603242-49603261 | MS.gene84452:CDS | 45.0% | |
| GATAAAGGAGATGCCCAATG+TGG | + | chr7.4:49603044-49603063 | MS.gene84452:CDS | 45.0% | |
| GCATCTCTAACTCTAGCTGA+AGG | + | chr7.4:49602985-49603004 | MS.gene84452:CDS | 45.0% | |
| ! | CTAGCTGAAGGTGCAGAATT+GGG | + | chr7.4:49602997-49603016 | MS.gene84452:CDS | 45.0% |
| ! | CTCCATCGGAAAGAGTTTTC+AGG | + | chr7.4:49602947-49602966 | MS.gene84452:CDS | 45.0% |
| ! | TCTAGCTGAAGGTGCAGAAT+TGG | + | chr7.4:49602996-49603015 | MS.gene84452:CDS | 45.0% |
| AAGGTCATCAAGGAGGTTCG+AGG | + | chr7.4:49603201-49603220 | MS.gene84452:CDS | 50.0% | |
| CCTCTTACTGCTAGCACTCT+AGG | + | chr7.4:49602769-49602788 | MS.gene84452:CDS | 50.0% | |
| CTTTCCTTGCAGCTTGACCT+AGG | - | chr7.4:49602831-49602850 | None:intergenic | 50.0% | |
| GAAACCTAGGTCAAGCTGCA+AGG | + | chr7.4:49602824-49602843 | MS.gene84452:CDS | 50.0% | |
| ! | CCTAGAGTGCTAGCAGTAAG+AGG | - | chr7.4:49602772-49602791 | None:intergenic | 50.0% |
| !!! | TTCTGGCTTTTGCTCCTCCT+TGG | - | chr7.4:49603130-49603149 | None:intergenic | 50.0% |
| CAATCATCGTGGGCCTCCAT+CGG | + | chr7.4:49602933-49602952 | MS.gene84452:CDS | 55.0% | |
| CGATGGAGGCCCACGATGAT+TGG | - | chr7.4:49602935-49602954 | None:intergenic | 60.0% | |
| GGAGGCCCACGATGATTGGT+AGG | - | chr7.4:49602931-49602950 | None:intergenic | 60.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr7.4 | gene | 49602712 | 49603365 | 49602712 | ID=MS.gene84452 |
| chr7.4 | mRNA | 49602712 | 49603365 | 49602712 | ID=MS.gene84452.t1;Parent=MS.gene84452 |
| chr7.4 | exon | 49602712 | 49603365 | 49602712 | ID=MS.gene84452.t1.exon1;Parent=MS.gene84452.t1 |
| chr7.4 | CDS | 49602712 | 49603365 | 49602712 | ID=cds.MS.gene84452.t1;Parent=MS.gene84452.t1 |
| Gene Sequence |
| Protein sequence |
