Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene84463.t1 | XP_013444570.1 | 88.3 | 213 | 1 | 1 | 17 | 205 | 1 | 213 | 7.10E-74 | 287 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene84463.t1 | Q93VH6 | 58.0 | 162 | 35 | 5 | 17 | 145 | 1 | 162 | 4.7e-34 | 146.0 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene84463.t1 | A0A072TYG4 | 88.3 | 213 | 1 | 1 | 17 | 205 | 1 | 213 | 5.1e-74 | 287.0 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI:
| Gene1 | Gene2 | Type |
|---|---|---|
| MS.gene008298 | MS.gene84463 | PPI |
| MS.gene53861 | MS.gene84463 | PPI |
| MS.gene84463 | MS.gene04991 | PPI |
| MS.gene84463 | MS.gene53859 | PPI |
| MS.gene048687 | MS.gene84463 | PPI |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene84463.t1 | MTR_8g024650 | 88.732 | 213 | 0 | 1 | 17 | 205 | 1 | 213 | 6.38e-129 | 362 |
| MS.gene84463.t1 | MTR_1g080590 | 57.778 | 90 | 37 | 1 | 18 | 107 | 3 | 91 | 2.76e-27 | 103 |
| MS.gene84463.t1 | MTR_7g115410 | 58.209 | 67 | 28 | 0 | 34 | 100 | 8 | 74 | 5.12e-19 | 81.6 |
| MS.gene84463.t1 | MTR_3g092260 | 52.308 | 65 | 31 | 0 | 26 | 90 | 1 | 65 | 2.71e-12 | 63.2 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene84463.t1 | AT4G00850 | 54.667 | 225 | 64 | 8 | 17 | 205 | 1 | 223 | 4.94e-62 | 192 |
| MS.gene84463.t1 | AT4G00850 | 55.738 | 183 | 40 | 7 | 17 | 160 | 1 | 181 | 1.52e-49 | 160 |
| MS.gene84463.t1 | AT1G01160 | 52.577 | 194 | 52 | 9 | 18 | 175 | 4 | 193 | 1.26e-44 | 147 |
| MS.gene84463.t1 | AT1G01160 | 44.737 | 228 | 52 | 10 | 18 | 175 | 4 | 227 | 6.98e-38 | 131 |
| MS.gene84463.t1 | AT5G28640 | 58.750 | 80 | 32 | 1 | 18 | 97 | 3 | 81 | 4.74e-24 | 95.1 |
Find 61 sgRNAs with CRISPR-Local
Find 0 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TGACCACTACCTATTGAATT+TGG | 0.186564 | 8.3:+67848903 | None:intergenic |
| CCACCACCACGCCCGTCATT+TGG | 0.299029 | 8.3:+67841409 | None:intergenic |
| GAAGCTGATCCTAGCAATAT+TGG | 0.361857 | 8.3:-67842422 | MS.gene84463:CDS |
| TGGTTATGTTTGTTTGTTGT+GGG | 0.373642 | 8.3:+67844942 | None:intergenic |
| CTTGTTGCATCGCAGGGTGC+GGG | 0.395604 | 8.3:+67841674 | None:intergenic |
| GCAGGCTATGCAAGGTCAAA+TGG | 0.412135 | 8.3:-67841512 | MS.gene84463:intron |
| ATGTTTCATCCAAATTCAAT+AGG | 0.416219 | 8.3:-67848912 | MS.gene84463:intron |
| AAGCAAGATGCTTCGGAAGC+TGG | 0.420759 | 8.3:-67841382 | MS.gene84463:CDS |
| CATGCCGTTGTTTATCCCTC+CGG | 0.429178 | 8.3:+67841480 | None:intergenic |
| ATATTGGACAATCAAAATCT+TGG | 0.436651 | 8.3:-67842406 | MS.gene84463:CDS |
| CCTTGTTGCATCGCAGGGTG+CGG | 0.438789 | 8.3:+67841673 | None:intergenic |
| GCTTCGGAAGCTGGCACAGC+CGG | 0.439731 | 8.3:-67841373 | MS.gene84463:CDS |
| GTTATGCGCAGCGGCGGCAG+AGG | 0.442288 | 8.3:+67841333 | None:intergenic |
| CCAAATGACGGGCGTGGTGG+TGG | 0.443789 | 8.3:-67841409 | MS.gene84463:CDS |
| GGAGGCAAGGCCGGTGTTTG+TGG | 0.444812 | 8.3:+67841797 | None:intergenic |
| TAGAATCCTTGTTGCATCGC+AGG | 0.458140 | 8.3:+67841667 | None:intergenic |
| ATTCTATATGCAACATCCTC+AGG | 0.459589 | 8.3:-67841650 | MS.gene84463:CDS |
| TGTGGGAATGAAGGCATCAT+AGG | 0.467268 | 8.3:+67844959 | None:intergenic |
| GTGGTTATGTTTGTTTGTTG+TGG | 0.473393 | 8.3:+67844941 | None:intergenic |
| GCTCTCGGAGGTAGCGGCAG+TGG | 0.475246 | 8.3:-67841436 | MS.gene84463:CDS |
| ATGCCGTTGTTTATCCCTCC+GGG | 0.482314 | 8.3:+67841481 | None:intergenic |
| GCTGGCACAGCCGGTGGAGA+CGG | 0.486755 | 8.3:-67841364 | MS.gene84463:CDS |
| TTTGTTTGTTGTGGGAATGA+AGG | 0.492115 | 8.3:+67844950 | None:intergenic |
| TTTACCTGTGGAGGCAAGGC+CGG | 0.494062 | 8.3:+67841788 | None:intergenic |
| ATGCACAACGAGGCTGCTCT+CGG | 0.494172 | 8.3:-67841451 | MS.gene84463:CDS |
| TTTGATTGTCCAATATTGCT+AGG | 0.496450 | 8.3:+67842413 | None:intergenic |
| AATTCTTCTGAAGCTGAGCT+TGG | 0.501703 | 8.3:+67841852 | None:intergenic |
| CAGGCTATGCAAGGTCAAAT+GGG | 0.504852 | 8.3:-67841511 | MS.gene84463:intron |
| TGAGCCATTGCTGCAGCCTG+AGG | 0.505768 | 8.3:+67841634 | None:intergenic |
| GCAGTGGTGGTCCAAATGAC+GGG | 0.506908 | 8.3:-67841420 | MS.gene84463:CDS |
| CTCGGAGGTAGCGGCAGTGG+TGG | 0.512539 | 8.3:-67841433 | MS.gene84463:CDS |
| ACATCCTCAGGCTGCAGCAA+TGG | 0.512641 | 8.3:-67841638 | MS.gene84463:CDS |
| AGAGCAGCCTCGTTGTGCAT+TGG | 0.512772 | 8.3:+67841454 | None:intergenic |
| TGGAAGCAAGCAAGATGCTT+CGG | 0.514046 | 8.3:-67841389 | MS.gene84463:CDS |
| GGCAGTGGTGGTCCAAATGA+CGG | 0.522402 | 8.3:-67841421 | MS.gene84463:CDS |
| AGTGGAGATGCTTCAGAAGA+GGG | 0.524475 | 8.3:-67841310 | MS.gene84463:CDS |
| TTCAGTTAAATTAGATAACA+AGG | 0.528303 | 8.3:-67845014 | MS.gene84463:intron |
| TGCGGATTACCGAACTGCAT+TGG | 0.539793 | 8.3:+67841583 | None:intergenic |
| TCGGAAGCTGGCACAGCCGG+TGG | 0.555558 | 8.3:-67841370 | MS.gene84463:CDS |
| GAGGCTGCTCTCGGAGGTAG+CGG | 0.555703 | 8.3:-67841442 | MS.gene84463:CDS |
| TTGTTGCATCGCAGGGTGCG+GGG | 0.574679 | 8.3:+67841675 | None:intergenic |
| TCCACTGTTATGCGCAGCGG+CGG | 0.576802 | 8.3:+67841327 | None:intergenic |
| GGTGGTCCAAATGACGGGCG+TGG | 0.578615 | 8.3:-67841415 | MS.gene84463:CDS |
| CCGCACCCTGCGATGCAACA+AGG | 0.581072 | 8.3:-67841673 | MS.gene84463:CDS |
| AGAATCCTTGTTGCATCGCA+GGG | 0.581843 | 8.3:+67841668 | None:intergenic |
| AACACCGGCCTTGCCTCCAC+AGG | 0.583006 | 8.3:-67841792 | MS.gene84463:intron |
| GGTCCAAATGACGGGCGTGG+TGG | 0.589036 | 8.3:-67841412 | MS.gene84463:CDS |
| CAGTGGAGATGCTTCAGAAG+AGG | 0.596581 | 8.3:-67841311 | MS.gene84463:CDS |
| GGCATCATAGGAATCATTTG+CGG | 0.604370 | 8.3:+67844971 | None:intergenic |
| GGTTCCTTGACCGTCTCCAC+CGG | 0.605589 | 8.3:+67841354 | None:intergenic |
| GCCGCCGCTGCGCATAACAG+TGG | 0.609000 | 8.3:-67841328 | MS.gene84463:CDS |
| ACAGCCGGTGGAGACGGTCA+AGG | 0.624474 | 8.3:-67841358 | MS.gene84463:CDS |
| ATCTCCACTGTTATGCGCAG+CGG | 0.625555 | 8.3:+67841324 | None:intergenic |
| TGATGCTCAGCCACAAACAC+CGG | 0.631733 | 8.3:-67841807 | MS.gene84463:CDS |
| GCAGCAATGGCTCAGCAACA+AGG | 0.635958 | 8.3:-67841625 | MS.gene84463:CDS |
| GGTCAAATGGGACTTAGACC+CGG | 0.641612 | 8.3:-67841499 | MS.gene84463:CDS |
| AAATGGGACTTAGACCCGGA+GGG | 0.675424 | 8.3:-67841495 | MS.gene84463:CDS |
| CACAACGAGGCTGCTCTCGG+AGG | 0.679125 | 8.3:-67841448 | MS.gene84463:CDS |
| CATGCATCCAATGCACAACG+AGG | 0.699827 | 8.3:-67841461 | MS.gene84463:CDS |
| CAAATGGGACTTAGACCCGG+AGG | 0.712224 | 8.3:-67841496 | MS.gene84463:CDS |
| AGACCCGGAGGGATAAACAA+CGG | 0.721572 | 8.3:-67841484 | MS.gene84463:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr8.3 | gene | 67841303 | 67848934 | 67841303 | ID=MS.gene84463 |
| chr8.3 | mRNA | 67841303 | 67848934 | 67841303 | ID=MS.gene84463.t1;Parent=MS.gene84463 |
| chr8.3 | exon | 67848913 | 67848934 | 67848913 | ID=MS.gene84463.t1.exon1;Parent=MS.gene84463.t1 |
| chr8.3 | CDS | 67848913 | 67848934 | 67848913 | ID=cds.MS.gene84463.t1;Parent=MS.gene84463.t1 |
| chr8.3 | exon | 67844925 | 67845031 | 67844925 | ID=MS.gene84463.t1.exon2;Parent=MS.gene84463.t1 |
| chr8.3 | CDS | 67844925 | 67845031 | 67844925 | ID=cds.MS.gene84463.t1;Parent=MS.gene84463.t1 |
| chr8.3 | exon | 67842385 | 67842461 | 67842385 | ID=MS.gene84463.t1.exon3;Parent=MS.gene84463.t1 |
| chr8.3 | CDS | 67842385 | 67842461 | 67842385 | ID=cds.MS.gene84463.t1;Parent=MS.gene84463.t1 |
| chr8.3 | exon | 67841793 | 67841877 | 67841793 | ID=MS.gene84463.t1.exon4;Parent=MS.gene84463.t1 |
| chr8.3 | CDS | 67841793 | 67841877 | 67841793 | ID=cds.MS.gene84463.t1;Parent=MS.gene84463.t1 |
| chr8.3 | exon | 67841593 | 67841701 | 67841593 | ID=MS.gene84463.t1.exon5;Parent=MS.gene84463.t1 |
| chr8.3 | CDS | 67841593 | 67841701 | 67841593 | ID=cds.MS.gene84463.t1;Parent=MS.gene84463.t1 |
| chr8.3 | exon | 67841303 | 67841520 | 67841303 | ID=MS.gene84463.t1.exon6;Parent=MS.gene84463.t1 |
| chr8.3 | CDS | 67841303 | 67841520 | 67841303 | ID=cds.MS.gene84463.t1;Parent=MS.gene84463.t1 |
| Gene Sequence |
| Protein sequence |
