Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene84841.t1 | XP_003627563.2 | 100 | 230 | 0 | 0 | 1 | 230 | 763 | 992 | 4.00E-118 | 434.1 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene84841.t1 | Q6EVK6 | 83.9 | 230 | 37 | 0 | 1 | 230 | 757 | 986 | 2.0e-102 | 373.2 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene84841.t1 | G7LBQ1 | 100.0 | 230 | 0 | 0 | 1 | 230 | 763 | 992 | 2.9e-118 | 434.1 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene050361 | MS.gene84841 | 0.812851 | 3.44E-51 | -1.69E-46 |
| MS.gene052609 | MS.gene84841 | 0.820143 | 8.01E-53 | -1.69E-46 |
| MS.gene053486 | MS.gene84841 | 0.827506 | 1.51E-54 | -1.69E-46 |
| MS.gene056507 | MS.gene84841 | 0.811861 | 5.66E-51 | -1.69E-46 |
| MS.gene060349 | MS.gene84841 | 0.824963 | 6.07E-54 | -1.69E-46 |
| MS.gene060350 | MS.gene84841 | 0.802258 | 6.11E-49 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene84841.t1 | MTR_8g030550 | 100.000 | 230 | 0 | 0 | 1 | 230 | 763 | 992 | 9.67e-157 | 475 |
| MS.gene84841.t1 | MTR_8g030550 | 100.000 | 230 | 0 | 0 | 1 | 230 | 763 | 992 | 1.43e-155 | 475 |
| MS.gene84841.t1 | MTR_8g030550 | 100.000 | 230 | 0 | 0 | 1 | 230 | 763 | 992 | 2.69e-155 | 476 |
| MS.gene84841.t1 | MTR_7g078090 | 87.446 | 231 | 28 | 1 | 1 | 230 | 769 | 999 | 6.95e-132 | 410 |
| MS.gene84841.t1 | MTR_4g118720 | 28.507 | 221 | 131 | 5 | 13 | 230 | 814 | 1010 | 1.78e-13 | 69.7 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene84841.t1 | AT2G46020 | 83.913 | 230 | 37 | 0 | 1 | 230 | 757 | 986 | 1.50e-123 | 386 |
| MS.gene84841.t1 | AT2G46020 | 83.913 | 230 | 37 | 0 | 1 | 230 | 757 | 986 | 1.50e-123 | 386 |
| MS.gene84841.t1 | AT2G46020 | 83.913 | 230 | 37 | 0 | 1 | 230 | 757 | 986 | 1.50e-123 | 386 |
| MS.gene84841.t1 | AT2G46020 | 83.913 | 230 | 37 | 0 | 1 | 230 | 757 | 986 | 1.50e-123 | 386 |
| MS.gene84841.t1 | AT2G46020 | 83.913 | 230 | 37 | 0 | 1 | 230 | 757 | 986 | 1.50e-123 | 386 |
| MS.gene84841.t1 | AT2G46020 | 83.913 | 230 | 37 | 0 | 1 | 230 | 757 | 986 | 1.50e-123 | 386 |
| MS.gene84841.t1 | AT2G28290 | 28.495 | 186 | 108 | 4 | 46 | 230 | 598 | 759 | 9.23e-11 | 61.6 |
| MS.gene84841.t1 | AT2G28290 | 28.495 | 186 | 108 | 4 | 46 | 230 | 598 | 759 | 9.24e-11 | 61.6 |
| MS.gene84841.t1 | AT2G28290 | 28.495 | 186 | 108 | 4 | 46 | 230 | 599 | 760 | 9.24e-11 | 61.6 |
| MS.gene84841.t1 | AT2G28290 | 28.495 | 186 | 108 | 4 | 46 | 230 | 598 | 759 | 9.41e-11 | 61.6 |
| MS.gene84841.t1 | AT2G28290 | 28.495 | 186 | 108 | 4 | 46 | 230 | 598 | 759 | 9.41e-11 | 61.6 |
| MS.gene84841.t1 | AT2G28290 | 28.495 | 186 | 108 | 4 | 46 | 230 | 599 | 760 | 9.41e-11 | 61.6 |
Find 46 sgRNAs with CRISPR-Local
Find 68 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TCAATGTTCCAGCTCGTAAT+AGG | 0.214844 | 8.1:-72485239 | None:intergenic |
| TGCGGATGCTGCACGGTTGC+AGG | 0.248464 | 8.1:+72484694 | MS.gene84841:CDS |
| GAAGAGTATCTACATAAATT+AGG | 0.304995 | 8.1:+72484614 | MS.gene84841:CDS |
| ACAATGATGTTGACAGATAT+AGG | 0.337249 | 8.1:+72484507 | MS.gene84841:CDS |
| AACTCAAATCTATATTTCTA+TGG | 0.339227 | 8.1:+72484336 | MS.gene84841:CDS |
| AACTTACGATATGGCCTATC+AGG | 0.340770 | 8.1:-72484236 | None:intergenic |
| ATGCCCGTACTGCTCGCAAC+AGG | 0.347417 | 8.1:+72484396 | MS.gene84841:CDS |
| GTCTTTCTGAAGAAGAAGTC+AGG | 0.389961 | 8.1:+72484894 | MS.gene84841:CDS |
| AGCCTAACAAACTTACGATA+TGG | 0.394999 | 8.1:-72484245 | None:intergenic |
| GGAGCAGCAGACTAGCATTC+CGG | 0.395107 | 8.1:+72484541 | MS.gene84841:CDS |
| CAATGTTCCAGCTCGTAATA+GGG | 0.402012 | 8.1:-72485238 | None:intergenic |
| CAGATATAGGGAGATGTTGC+TGG | 0.405041 | 8.1:+72484520 | MS.gene84841:CDS |
| GGCCATATCGTAAGTTTGTT+AGG | 0.405651 | 8.1:+72484243 | MS.gene84841:CDS |
| CCGGAGAGGAAGTGATGATT+AGG | 0.409531 | 8.1:+72484933 | MS.gene84841:CDS |
| AGATAATGGCAATGCCTGAT+AGG | 0.420128 | 8.1:+72484222 | None:intergenic |
| GAGCAGCAGACTAGCATTCC+GGG | 0.422011 | 8.1:+72484542 | MS.gene84841:CDS |
| TATGTAGATACTCTTCAGTC+TGG | 0.431556 | 8.1:-72484607 | None:intergenic |
| CAATGATGTTGACAGATATA+GGG | 0.437320 | 8.1:+72484508 | MS.gene84841:CDS |
| AGGGCTGCTGCTGCTTGTGC+CGG | 0.458998 | 8.1:+72484914 | MS.gene84841:CDS |
| CGCACATGCAGTGAATGAAA+TGG | 0.486137 | 8.1:+72485200 | MS.gene84841:CDS |
| CCCGTACTGCTCGCAACAGG+GGG | 0.495772 | 8.1:+72484399 | MS.gene84841:CDS |
| TGCCCGTACTGCTCGCAACA+GGG | 0.520777 | 8.1:+72484397 | MS.gene84841:CDS |
| AAGCAGCTGCGGATGCTGCA+CGG | 0.522912 | 8.1:+72484687 | MS.gene84841:CDS |
| CCTAATCATCACTTCCTCTC+CGG | 0.524612 | 8.1:-72484933 | None:intergenic |
| GCCCGTACTGCTCGCAACAG+GGG | 0.534723 | 8.1:+72484398 | MS.gene84841:CDS |
| AGAAACTTCTTGAGGCACAC+TGG | 0.542348 | 8.1:+72484363 | MS.gene84841:CDS |
| GGAACTTGTAAGACAAGTGC+AGG | 0.543292 | 8.1:+72484286 | MS.gene84841:CDS |
| GAAACTTCTTGAGGCACACT+GGG | 0.544904 | 8.1:+72484364 | MS.gene84841:CDS |
| GCTGTGTGAACGTCAGCGTA+TGG | 0.548927 | 8.1:+72484265 | MS.gene84841:CDS |
| TGCTGCTGCTTGTGCCGGAG+AGG | 0.562766 | 8.1:+72484919 | MS.gene84841:CDS |
| TTGACGGATGAACTGTCTCT+AGG | 0.569365 | 8.1:-72484980 | None:intergenic |
| TATCTCTCTGCAGCATCACC+CGG | 0.574204 | 8.1:-72484560 | None:intergenic |
| ATATCATGAGAGGATGTTGA+GGG | 0.577857 | 8.1:+72484430 | MS.gene84841:CDS |
| CAACCATCCCTATTACGAGC+TGG | 0.597783 | 8.1:+72485231 | MS.gene84841:CDS |
| AAATCAACAGGAAGTAGAGG+AGG | 0.597876 | 8.1:+72484658 | MS.gene84841:CDS |
| GGCGTCTCAGAAAGCTGCAA+GGG | 0.599846 | 8.1:+72484307 | MS.gene84841:CDS |
| GGAGGCAGCAAAAGCAGCTG+CGG | 0.600888 | 8.1:+72484676 | MS.gene84841:CDS |
| AATATCATGAGAGGATGTTG+AGG | 0.602609 | 8.1:+72484429 | MS.gene84841:CDS |
| TCTTTCTGAAGAAGAAGTCA+GGG | 0.608858 | 8.1:+72484895 | MS.gene84841:CDS |
| TTTCATTCACTGCATGTGCG+AGG | 0.613575 | 8.1:-72485197 | None:intergenic |
| AAGATGACGACAGAAGCAAA+AGG | 0.625349 | 8.1:+72484468 | MS.gene84841:CDS |
| GTTCCAGCTCGTAATAGGGA+TGG | 0.634643 | 8.1:-72485234 | None:intergenic |
| AGGCGTCTCAGAAAGCTGCA+AGG | 0.638704 | 8.1:+72484306 | MS.gene84841:CDS |
| ATGGCGTAAGAAACTTCTTG+AGG | 0.650602 | 8.1:+72484355 | MS.gene84841:CDS |
| GGGTGGGTAAATATCATGAG+AGG | 0.675920 | 8.1:+72484420 | MS.gene84841:CDS |
| CCGTACTGCTCGCAACAGGG+GGG | 0.713270 | 8.1:+72484400 | MS.gene84841:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !!! | CTAATTTGTATAGAAAATAT+TGG | - | chr8.1:72484775-72484794 | None:intergenic | 15.0% |
| !! | AATCAAGATATACAAACATA+TGG | - | chr8.1:72485031-72485050 | None:intergenic | 20.0% |
| !! | GTAATTGTAATGAAATTCAT+TGG | + | chr8.1:72485083-72485102 | MS.gene84841:intron | 20.0% |
| !!! | AACTCAAATCTATATTTCTA+TGG | + | chr8.1:72484336-72484355 | MS.gene84841:CDS | 20.0% |
| !!! | ATTAATTGTTTTTGACCAAA+GGG | + | chr8.1:72484872-72484891 | MS.gene84841:intron | 20.0% |
| !!! | TTCTATACAAATTAGTTTTG+AGG | + | chr8.1:72484780-72484799 | MS.gene84841:intron | 20.0% |
| !!! | CATTAATTGTTTTTGACCAA+AGG | + | chr8.1:72484871-72484890 | MS.gene84841:intron | 25.0% |
| !!! | GAAGAGTATCTACATAAATT+AGG | + | chr8.1:72484614-72484633 | MS.gene84841:CDS | 25.0% |
| !!! | GTCAACATCATTGTTTTTTA+AGG | - | chr8.1:72484501-72484520 | None:intergenic | 25.0% |
| ACAATGATGTTGACAGATAT+AGG | + | chr8.1:72484507-72484526 | MS.gene84841:CDS | 30.0% | |
| CAATGATGTTGACAGATATA+GGG | + | chr8.1:72484508-72484527 | MS.gene84841:CDS | 30.0% | |
| ! | AACAAAAACTCACTTGTTGA+CGG | - | chr8.1:72484999-72485018 | None:intergenic | 30.0% |
| !! | CTATATGAACTAGTTCTGTA+AGG | + | chr8.1:72484829-72484848 | MS.gene84841:intron | 30.0% |
| !! | TAAGTTAATTTGCTTCTTCC+AGG | + | chr8.1:72485166-72485185 | MS.gene84841:intron | 30.0% |
| !! | TTTTGAGGATCTGATTTTGA+TGG | + | chr8.1:72484795-72484814 | MS.gene84841:intron | 30.0% |
| AATATCATGAGAGGATGTTG+AGG | + | chr8.1:72484429-72484448 | MS.gene84841:CDS | 35.0% | |
| ACACAAAAATGCATGAGCAA+CGG | + | chr8.1:72485059-72485078 | MS.gene84841:intron | 35.0% | |
| AGCCTAACAAACTTACGATA+TGG | - | chr8.1:72484248-72484267 | None:intergenic | 35.0% | |
| ATATCATGAGAGGATGTTGA+GGG | + | chr8.1:72484430-72484449 | MS.gene84841:CDS | 35.0% | |
| CAAAAATCAACAGGAAGTAG+AGG | + | chr8.1:72484655-72484674 | MS.gene84841:CDS | 35.0% | |
| TATGTAGATACTCTTCAGTC+TGG | - | chr8.1:72484610-72484629 | None:intergenic | 35.0% | |
| ! | GATGATTAGGAATCGTTTTC+TGG | + | chr8.1:72484946-72484965 | MS.gene84841:CDS | 35.0% |
| ! | TCTTTCTGAAGAAGAAGTCA+GGG | + | chr8.1:72484895-72484914 | MS.gene84841:CDS | 35.0% |
| !! | AAGACTTTTTCTCAGAACGT+TGG | - | chr8.1:72484743-72484762 | None:intergenic | 35.0% |
| !!! | CTCTACTTCCTGTTGATTTT+TGG | - | chr8.1:72484657-72484676 | None:intergenic | 35.0% |
| AAATCAACAGGAAGTAGAGG+AGG | + | chr8.1:72484658-72484677 | MS.gene84841:CDS | 40.0% | |
| AACAGCTGCCAAAAATCAAC+AGG | + | chr8.1:72484646-72484665 | MS.gene84841:CDS | 40.0% | |
| AACTTACGATATGGCCTATC+AGG | - | chr8.1:72484239-72484258 | None:intergenic | 40.0% | |
| AAGATGACGACAGAAGCAAA+AGG | + | chr8.1:72484468-72484487 | MS.gene84841:CDS | 40.0% | |
| ATGGCGTAAGAAACTTCTTG+AGG | + | chr8.1:72484355-72484374 | MS.gene84841:CDS | 40.0% | |
| CAATGTTCCAGCTCGTAATA+GGG | - | chr8.1:72485241-72485260 | None:intergenic | 40.0% | |
| TCAATGTTCCAGCTCGTAAT+AGG | - | chr8.1:72485242-72485261 | None:intergenic | 40.0% | |
| TCTTCTTCAGAAAGACCCTT+TGG | - | chr8.1:72484890-72484909 | None:intergenic | 40.0% | |
| ! | GGCCATATCGTAAGTTTGTT+AGG | + | chr8.1:72484243-72484262 | MS.gene84841:CDS | 40.0% |
| ! | GTCTTTCTGAAGAAGAAGTC+AGG | + | chr8.1:72484894-72484913 | MS.gene84841:CDS | 40.0% |
| AGAAACTTCTTGAGGCACAC+TGG | + | chr8.1:72484363-72484382 | MS.gene84841:CDS | 45.0% | |
| ATGTGCGAGGTTGTAATACC+TGG | - | chr8.1:72485187-72485206 | None:intergenic | 45.0% | |
| CAGATATAGGGAGATGTTGC+TGG | + | chr8.1:72484520-72484539 | MS.gene84841:CDS | 45.0% | |
| CCTAATCATCACTTCCTCTC+CGG | - | chr8.1:72484936-72484955 | None:intergenic | 45.0% | |
| CGCACATGCAGTGAATGAAA+TGG | + | chr8.1:72485200-72485219 | MS.gene84841:CDS | 45.0% | |
| GAAACTTCTTGAGGCACACT+GGG | + | chr8.1:72484364-72484383 | MS.gene84841:CDS | 45.0% | |
| GGAACTTGTAAGACAAGTGC+AGG | + | chr8.1:72484286-72484305 | MS.gene84841:CDS | 45.0% | |
| GGGTGGGTAAATATCATGAG+AGG | + | chr8.1:72484420-72484439 | MS.gene84841:CDS | 45.0% | |
| TTTCATTCACTGCATGTGCG+AGG | - | chr8.1:72485200-72485219 | None:intergenic | 45.0% | |
| ! | TTGACGGATGAACTGTCTCT+AGG | - | chr8.1:72484983-72485002 | None:intergenic | 45.0% |
| CAACCATCCCTATTACGAGC+TGG | + | chr8.1:72485231-72485250 | MS.gene84841:CDS | 50.0% | |
| CCGGAGAGGAAGTGATGATT+AGG | + | chr8.1:72484933-72484952 | MS.gene84841:CDS | 50.0% | |
| GTTCCAGCTCGTAATAGGGA+TGG | - | chr8.1:72485237-72485256 | None:intergenic | 50.0% | |
| TATCTCTCTGCAGCATCACC+CGG | - | chr8.1:72484563-72484582 | None:intergenic | 50.0% | |
| AGGCGTCTCAGAAAGCTGCA+AGG | + | chr8.1:72484306-72484325 | MS.gene84841:CDS | 55.0% | |
| GAGCAGCAGACTAGCATTCC+GGG | + | chr8.1:72484542-72484561 | MS.gene84841:CDS | 55.0% | |
| GCTGTGTGAACGTCAGCGTA+TGG | + | chr8.1:72484265-72484284 | MS.gene84841:CDS | 55.0% | |
| GGAGCAGCAGACTAGCATTC+CGG | + | chr8.1:72484541-72484560 | MS.gene84841:CDS | 55.0% | |
| GGCGTCTCAGAAAGCTGCAA+GGG | + | chr8.1:72484307-72484326 | MS.gene84841:CDS | 55.0% | |
| AAGCAGCTGCGGATGCTGCA+CGG | + | chr8.1:72484687-72484706 | MS.gene84841:CDS | 60.0% | |
| ATGCCCGTACTGCTCGCAAC+AGG | + | chr8.1:72484396-72484415 | MS.gene84841:CDS | 60.0% | |
| GGAGGCAGCAAAAGCAGCTG+CGG | + | chr8.1:72484676-72484695 | MS.gene84841:CDS | 60.0% | |
| TGCCCGTACTGCTCGCAACA+GGG | + | chr8.1:72484397-72484416 | MS.gene84841:CDS | 60.0% | |
| ACTGCTCGCAACAGGGGGGT+GGG | + | chr8.1:72484404-72484423 | MS.gene84841:CDS | 65.0% | |
| CCCCCCTGTTGCGAGCAGTA+CGG | - | chr8.1:72484403-72484422 | None:intergenic | 65.0% | |
| CCCCCTGTTGCGAGCAGTAC+GGG | - | chr8.1:72484402-72484421 | None:intergenic | 65.0% | |
| CCCGTACTGCTCGCAACAGG+GGG | + | chr8.1:72484399-72484418 | MS.gene84841:CDS | 65.0% | |
| CCGTACTGCTCGCAACAGGG+GGG | + | chr8.1:72484400-72484419 | MS.gene84841:CDS | 65.0% | |
| GCCCGTACTGCTCGCAACAG+GGG | + | chr8.1:72484398-72484417 | MS.gene84841:CDS | 65.0% | |
| TACTGCTCGCAACAGGGGGG+TGG | + | chr8.1:72484403-72484422 | MS.gene84841:CDS | 65.0% | |
| TGCGGATGCTGCACGGTTGC+AGG | + | chr8.1:72484694-72484713 | MS.gene84841:CDS | 65.0% | |
| ! | AGGGCTGCTGCTGCTTGTGC+CGG | + | chr8.1:72484914-72484933 | MS.gene84841:CDS | 65.0% |
| ! | TGCTGCTGCTTGTGCCGGAG+AGG | + | chr8.1:72484919-72484938 | MS.gene84841:CDS | 65.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr8.1 | gene | 72484227 | 72485275 | 72484227 | ID=MS.gene84841 |
| chr8.1 | mRNA | 72484227 | 72485275 | 72484227 | ID=MS.gene84841.t1;Parent=MS.gene84841 |
| chr8.1 | exon | 72484227 | 72484715 | 72484227 | ID=MS.gene84841.t1.exon1;Parent=MS.gene84841.t1 |
| chr8.1 | CDS | 72484227 | 72484715 | 72484227 | ID=cds.MS.gene84841.t1;Parent=MS.gene84841.t1 |
| chr8.1 | exon | 72484893 | 72485005 | 72484893 | ID=MS.gene84841.t1.exon2;Parent=MS.gene84841.t1 |
| chr8.1 | CDS | 72484893 | 72485005 | 72484893 | ID=cds.MS.gene84841.t1;Parent=MS.gene84841.t1 |
| chr8.1 | exon | 72485188 | 72485275 | 72485188 | ID=MS.gene84841.t1.exon3;Parent=MS.gene84841.t1 |
| chr8.1 | CDS | 72485188 | 72485275 | 72485188 | ID=cds.MS.gene84841.t1;Parent=MS.gene84841.t1 |
| Gene Sequence |
| Protein sequence |
