AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene85211

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene85211.t1 MTR_2g100060 93.939 264 11 2 1 263 1 260 1.01e-147 413
MS.gene85211.t1 MTR_8g016370 47.104 259 97 6 5 263 7 225 4.65e-71 218
MS.gene85211.t1 MTR_3g104460 42.481 266 133 8 7 262 4 259 8.63e-67 208
MS.gene85211.t1 MTR_5g087760 30.453 243 141 7 1 227 1 231 6.04e-30 113
MS.gene85211.t1 MTR_5g076170 34.343 198 121 4 31 226 47 237 4.12e-24 97.8
MS.gene85211.t1 MTR_2g012550 29.864 221 134 6 18 230 40 247 4.85e-23 95.1
MS.gene85211.t1 MTR_3g078800 29.915 234 152 7 1 227 1 229 5.84e-23 95.1
MS.gene85211.t1 MTR_7g106000 29.258 229 143 10 34 253 7 225 5.56e-19 83.6
MS.gene85211.t1 MTR_3g063310 32.867 143 92 3 86 226 86 226 6.66e-19 83.6
MS.gene85211.t1 MTR_1g077560 30.000 190 130 2 61 249 60 247 7.21e-18 80.9
MS.gene85211.t1 MTR_7g105970 29.245 212 126 10 53 254 29 226 1.87e-15 73.9
MS.gene85211.t1 MTR_4g119428 28.293 205 126 5 41 227 12 213 3.54e-15 73.2
MS.gene85211.t1 MTR_7g118270 27.895 190 127 4 69 254 46 229 8.53e-14 69.3
MS.gene85211.t1 MTR_3g078820 29.078 141 99 1 87 227 44 183 9.74e-14 68.6
MS.gene85211.t1 MTR_1g057270 25.824 182 123 6 84 259 72 247 1.46e-13 68.9
MS.gene85211.t1 MTR_6g071410 25.786 159 113 4 61 215 2 159 1.16e-12 65.9
MS.gene85211.t1 MTR_6g070890 25.786 159 113 4 61 215 2 159 1.16e-12 65.9
MS.gene85211.t1 MTR_2g011350 23.429 175 127 2 80 254 35 202 1.36e-12 65.5
MS.gene85211.t1 MTR_3g062470 22.905 179 132 4 62 238 66 240 6.46e-12 64.3
MS.gene85211.t1 MTR_6g471090 27.160 162 99 5 60 215 9 157 1.01e-11 63.2
MS.gene85211.t1 MTR_7g106050 25.989 177 118 5 52 226 12 177 2.04e-11 62.4
MS.gene85211.t1 MTR_7g106010 26.407 231 152 8 34 253 3 226 2.26e-11 62.4
MS.gene85211.t1 MTR_1g057400 26.147 218 136 9 49 253 3 208 2.35e-11 62.0
MS.gene85211.t1 MTR_3g074150 21.827 197 138 4 37 227 36 222 2.51e-11 62.8
MS.gene85211.t1 MTR_6g070900 26.882 186 116 8 62 235 2 179 2.60e-11 62.0
MS.gene85211.t1 MTR_6g071400 26.882 186 116 8 62 235 2 179 2.60e-11 62.0
MS.gene85211.t1 MTR_7g106030 26.887 212 133 9 54 252 27 229 5.28e-11 61.2
MS.gene85211.t1 MTR_3g092790 26.619 139 99 3 92 229 49 185 8.00e-11 60.5
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene85211.t1 AT1G17620 47.015 268 126 4 3 262 4 263 3.86e-64 201
MS.gene85211.t1 AT5G11890 36.271 295 146 10 1 262 1 286 1.60e-44 152
MS.gene85211.t1 AT2G27080 31.557 244 142 7 1 227 1 236 5.65e-31 116
MS.gene85211.t1 AT2G27080 31.557 244 142 7 1 227 1 236 5.65e-31 116
MS.gene85211.t1 AT1G65690 31.056 161 107 3 68 226 68 226 6.28e-21 89.4
MS.gene85211.t1 AT5G36970 29.126 206 138 5 54 258 50 248 3.90e-18 81.6
MS.gene85211.t1 AT2G35980 27.982 218 151 5 40 253 11 226 1.07e-17 80.1
MS.gene85211.t1 AT5G21130 26.804 194 137 4 37 226 64 256 3.15e-17 79.7
MS.gene85211.t1 AT3G11650 26.131 199 139 6 60 254 45 239 3.66e-16 75.9
MS.gene85211.t1 AT1G54540 28.125 192 135 2 59 249 47 236 1.64e-15 74.3
MS.gene85211.t1 AT5G06320 27.660 188 126 5 70 253 49 230 2.93e-14 70.5
MS.gene85211.t1 AT4G01410 23.737 198 138 5 59 254 41 227 7.84e-13 66.6
MS.gene85211.t1 AT4G01110 25.888 197 122 5 75 261 74 256 2.80e-12 65.5
MS.gene85211.t1 AT5G22870 23.158 190 133 3 68 254 28 207 4.94e-12 63.9
MS.gene85211.t1 AT2G46300 28.662 157 103 4 84 232 77 232 1.80e-11 63.2
MS.gene85211.t1 AT5G53730 25.269 186 126 5 72 254 38 213 8.11e-11 60.5

Find 85 sgRNAs with CRISPR-Local

Find 95 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TTCGAAGTTGACGCCGTTAC+TGG 0.277675 2.4:-4279302 None:intergenic
GCTGCTGCACCTTCTTCTTC+TGG 0.279365 2.4:+4278766 MS.gene85211:CDS
CTTCTCCTCCTCGGCGTAAC+GGG 0.331250 2.4:+4278813 MS.gene85211:CDS
ACGAAGGTGATATGTTTGTT+AGG 0.332211 2.4:-4278969 None:intergenic
TGCTTGACCAGTACTCAAAA+TGG 0.356336 2.4:-4279099 None:intergenic
TTGGTCATGGGTTTGGCGGC+GGG 0.359331 2.4:-4278603 None:intergenic
CGAAGGTGATATGTTTGTTA+GGG 0.379442 2.4:-4278968 None:intergenic
AGCAGCAACGGCGGCGGGTA+CGG 0.382447 2.4:-4278749 None:intergenic
CTTCATCAATTAAATTTAAA+CGG 0.385881 2.4:-4279369 None:intergenic
GACACCGTTGCCGTTGGTCA+TGG 0.391670 2.4:-4278616 None:intergenic
GTTGCCGTTGGTCATGGGTT+TGG 0.392896 2.4:-4278610 None:intergenic
TGGAGGAGGAGGTGAGGTTG+AGG 0.394091 2.4:-4278905 None:intergenic
TGAGTTGAGGGTGGAGGAGG+AGG 0.397719 2.4:-4278916 None:intergenic
GGTGCAGCAGCAACGGCGGC+GGG 0.411032 2.4:-4278754 None:intergenic
GTTGGTCATGGGTTTGGCGG+CGG 0.419990 2.4:-4278604 None:intergenic
TACAAAAGTGAAGGCTAAAG+TGG 0.436063 2.4:+4279199 MS.gene85211:CDS
TTGATGTTAGGTTTAAGATC+TGG 0.438319 2.4:+4279339 MS.gene85211:CDS
CCTTCTCCTCCTCGGCGTAA+CGG 0.438746 2.4:+4278812 MS.gene85211:CDS
TGAGGGTGGAGGAGGAGGTG+AGG 0.440148 2.4:-4278911 None:intergenic
TTAAGTGTGATGTTGATGTT+AGG 0.446188 2.4:+4279327 MS.gene85211:CDS
GGAAAGTTTGAGAGATGTTA+CGG 0.447386 2.4:-4278880 None:intergenic
ACACCGTTGCCGTTGGTCAT+GGG 0.448685 2.4:-4278615 None:intergenic
GGAGGAGGTGAGGTTGAGGT+AGG 0.450411 2.4:-4278901 None:intergenic
GAAGGAGGATGAGAAGTATG+AGG 0.450998 2.4:-4278794 None:intergenic
TTTAGCGTTGAAAGTGAAGT+TGG 0.454211 2.4:+4279175 MS.gene85211:CDS
AACGGCGGCGGGTACGGCGG+TGG 0.458183 2.4:-4278743 None:intergenic
GTAACTCTTATTCCAACGTT+AGG 0.471241 2.4:-4279236 None:intergenic
GGCTGGAAATGATGGGTTTG+CGG 0.475941 2.4:-4278667 None:intergenic
AGGTGCAGCAGCAACGGCGG+CGG 0.477876 2.4:-4278755 None:intergenic
AGAAGAAGGTGCAGCAGCAA+CGG 0.479402 2.4:-4278761 None:intergenic
TTAGGTTTAAGATCTGGAAA+TGG 0.481195 2.4:+4279345 MS.gene85211:CDS
TTCTTGCCATGTGTGAAAGT+TGG 0.485690 2.4:-4279053 None:intergenic
GAGAGAATTGTGATGGTGGT+TGG 0.496275 2.4:-4278999 None:intergenic
CTCATCCTCCTTCTCCTCCT+CGG 0.497763 2.4:+4278804 MS.gene85211:CDS
GCCGTTGGTCATGGGTTTGG+CGG 0.502360 2.4:-4278607 None:intergenic
TTTGAGAGATGTTACGGTGA+AGG 0.503461 2.4:-4278874 None:intergenic
GACGTTGTGGACGGTAGGTA+GGG 0.512263 2.4:-4278719 None:intergenic
TGACGTTGTGGACGGTAGGT+AGG 0.513516 2.4:-4278720 None:intergenic
CCGTTACGCCGAGGAGGAGA+AGG 0.513542 2.4:-4278812 None:intergenic
ACTGGTTTCTTATTACCGAC+AGG 0.516093 2.4:-4279284 None:intergenic
GTTTGAGAGAATTGTGATGG+TGG 0.523617 2.4:-4279003 None:intergenic
AGCAACGGCGGCGGGTACGG+CGG 0.526300 2.4:-4278746 None:intergenic
GCCATGTGTGAAAGTTGGTA+TGG 0.529809 2.4:-4279048 None:intergenic
GCCACAAAAGCTCAACTCTA+CGG 0.539658 2.4:+4278687 MS.gene85211:CDS
GTTGTTTGAGAGAATTGTGA+TGG 0.543870 2.4:-4279006 None:intergenic
TACGGTGAAGGAAGGACGGT+GGG 0.551852 2.4:-4278862 None:intergenic
TGAAGGAAGGACGGTGGGGA+CGG 0.553633 2.4:-4278857 None:intergenic
AACGGTGTCACCAACGGTAA+CGG 0.557429 2.4:+4278630 MS.gene85211:CDS
AGAGATGTTACGGTGAAGGA+AGG 0.565773 2.4:-4278870 None:intergenic
TTGTGGACGGTAGGTAGGGC+GGG 0.566489 2.4:-4278715 None:intergenic
GTCACCAACGGTAACGGTAA+CGG 0.571662 2.4:+4278636 MS.gene85211:CDS
GTTGGTGACACCGTTGCCGT+TGG 0.575232 2.4:-4278622 None:intergenic
GACGGTGGGGACGGTAGATG+AGG 0.578731 2.4:-4278848 None:intergenic
GTTGTGGACGGTAGGTAGGG+CGG 0.578929 2.4:-4278716 None:intergenic
AACGGCAACGGTGTCACCAA+CGG 0.582278 2.4:+4278624 MS.gene85211:CDS
AGAAGGTGCAGCAGCAACGG+CGG 0.583077 2.4:-4278758 None:intergenic
GAAGTTGGATACAAAAGTGA+AGG 0.584664 2.4:+4279190 MS.gene85211:CDS
ATTAGAATCAGACGCTTCCA+CGG 0.587238 2.4:+4279121 MS.gene85211:CDS
GAATTGTGATGGTGGTTGGT+TGG 0.587560 2.4:-4278995 None:intergenic
CATGTTCTTCTTCAGCTCCG+TGG 0.589253 2.4:-4279138 None:intergenic
GTTAGAGTTCATGTTCCTGT+CGG 0.590463 2.4:+4279269 MS.gene85211:CDS
AAACCCATGACCAACGGCAA+CGG 0.592031 2.4:+4278612 MS.gene85211:CDS
GTACGGCGGTGGTGACGTTG+TGG 0.593231 2.4:-4278732 None:intergenic
GAGGAAGAACCAGAAGAAGA+AGG 0.593450 2.4:-4278775 None:intergenic
AACAACATCAACATCGGTGA+AGG 0.594037 2.4:+4279023 MS.gene85211:CDS
GTGGTGACGTTGTGGACGGT+AGG 0.597449 2.4:-4278724 None:intergenic
GCCGCCAAACCCATGACCAA+CGG 0.597855 2.4:+4278606 MS.gene85211:CDS
AAAATGAAAACACCTAACGT+TGG 0.600696 2.4:+4279224 MS.gene85211:CDS
TGGACGGTAGGTAGGGCGGG+TGG 0.609236 2.4:-4278712 None:intergenic
ACAAAAGTGAAGGCTAAAGT+GGG 0.615350 2.4:+4279200 MS.gene85211:CDS
CTCTCAAACAACATCAACAT+CGG 0.615638 2.4:+4279017 MS.gene85211:CDS
GGCGGTGGTGACGTTGTGGA+CGG 0.618413 2.4:-4278728 None:intergenic
GACACCGTTACCGTTACCGT+TGG 0.628131 2.4:-4278640 None:intergenic
GGAATAAGAGTTACATGTGA+CGG 0.629815 2.4:+4279245 MS.gene85211:CDS
TTACGCCGAGGAGGAGAAGG+AGG 0.630487 2.4:-4278809 None:intergenic
TGGAAATGATGGGTTTGCGG+CGG 0.637844 2.4:-4278664 None:intergenic
ACCATACCAACTTTCACACA+TGG 0.645797 2.4:+4279047 MS.gene85211:CDS
CGGTGCCCGTTACGCCGAGG+AGG 0.646188 2.4:-4278818 None:intergenic
ATGTTACGGTGAAGGAAGGA+CGG 0.655102 2.4:-4278866 None:intergenic
TTACGGTGAAGGAAGGACGG+TGG 0.673058 2.4:-4278863 None:intergenic
CGGTAATAAGAAACCAGTAA+CGG 0.676522 2.4:+4279289 MS.gene85211:CDS
AAGCGGTGCCCGTTACGCCG+AGG 0.677693 2.4:-4278821 None:intergenic
ACGGTAGATGAGGTAGAAAG+CGG 0.681553 2.4:-4278838 None:intergenic
GGTGGTTGGTTGGTAAACGA+AGG 0.694931 2.4:-4278985 None:intergenic
ACGGTGAAGGAAGGACGGTG+GGG 0.703670 2.4:-4278861 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
AAAATGAAAACACCTAACGT+TGG + chr2.4:4279224-4279243 MS.gene85211:CDS 30.0%
AAGAACATGAAGAGCAAAAA+CGG + chr2.4:4279152-4279171 MS.gene85211:CDS 30.0%
TTAAGTGTGATGTTGATGTT+AGG + chr2.4:4279327-4279346 MS.gene85211:CDS 30.0%
! TTAGGTTTAAGATCTGGAAA+TGG + chr2.4:4279345-4279364 MS.gene85211:CDS 30.0%
! TTGATGTTAGGTTTAAGATC+TGG + chr2.4:4279339-4279358 MS.gene85211:CDS 30.0%
!! AAATCTTCCATTTTGAGTAC+TGG + chr2.4:4279092-4279111 MS.gene85211:CDS 30.0%
ACAAAAGTGAAGGCTAAAGT+GGG + chr2.4:4279200-4279219 MS.gene85211:CDS 35.0%
ACGAAGGTGATATGTTTGTT+AGG - chr2.4:4278972-4278991 None:intergenic 35.0%
CGGTAATAAGAAACCAGTAA+CGG + chr2.4:4279289-4279308 MS.gene85211:CDS 35.0%
CTCTCAAACAACATCAACAT+CGG + chr2.4:4279017-4279036 MS.gene85211:CDS 35.0%
GAAGTTGGATACAAAAGTGA+AGG + chr2.4:4279190-4279209 MS.gene85211:CDS 35.0%
GGAAAGTTTGAGAGATGTTA+CGG - chr2.4:4278883-4278902 None:intergenic 35.0%
GGAATAAGAGTTACATGTGA+CGG + chr2.4:4279245-4279264 MS.gene85211:CDS 35.0%
GTAACTCTTATTCCAACGTT+AGG - chr2.4:4279239-4279258 None:intergenic 35.0%
GTTGTTTGAGAGAATTGTGA+TGG - chr2.4:4279009-4279028 None:intergenic 35.0%
TACAAAAGTGAAGGCTAAAG+TGG + chr2.4:4279199-4279218 MS.gene85211:CDS 35.0%
! CGAAGGTGATATGTTTGTTA+GGG - chr2.4:4278971-4278990 None:intergenic 35.0%
! TTTAGCGTTGAAAGTGAAGT+TGG + chr2.4:4279175-4279194 MS.gene85211:CDS 35.0%
!!! GACGTTGAATTTTGAGTTGA+GGG - chr2.4:4278931-4278950 None:intergenic 35.0%
!!! TGACGTTGAATTTTGAGTTG+AGG - chr2.4:4278932-4278951 None:intergenic 35.0%
AACAACATCAACATCGGTGA+AGG + chr2.4:4279023-4279042 MS.gene85211:CDS 40.0%
ACCATACCAACTTTCACACA+TGG + chr2.4:4279047-4279066 MS.gene85211:CDS 40.0%
ATTAGAATCAGACGCTTCCA+CGG + chr2.4:4279121-4279140 MS.gene85211:CDS 40.0%
GTTAGAGTTCATGTTCCTGT+CGG + chr2.4:4279269-4279288 MS.gene85211:CDS 40.0%
GTTTGAGAGAATTGTGATGG+TGG - chr2.4:4279006-4279025 None:intergenic 40.0%
TGCTTGACCAGTACTCAAAA+TGG - chr2.4:4279102-4279121 None:intergenic 40.0%
TTCTTGCCATGTGTGAAAGT+TGG - chr2.4:4279056-4279075 None:intergenic 40.0%
TTTGAGAGATGTTACGGTGA+AGG - chr2.4:4278877-4278896 None:intergenic 40.0%
! ACTGGTTTCTTATTACCGAC+AGG - chr2.4:4279287-4279306 None:intergenic 40.0%
! CTTTTGTGGCTGGAAATGAT+GGG - chr2.4:4278677-4278696 None:intergenic 40.0%
!!! GTTGAATTTTGAGTTGAGGG+TGG - chr2.4:4278928-4278947 None:intergenic 40.0%
ACGGTAGATGAGGTAGAAAG+CGG - chr2.4:4278841-4278860 None:intergenic 45.0%
AGAGATGTTACGGTGAAGGA+AGG - chr2.4:4278873-4278892 None:intergenic 45.0%
ATGTTACGGTGAAGGAAGGA+CGG - chr2.4:4278869-4278888 None:intergenic 45.0%
GAGGAAGAACCAGAAGAAGA+AGG - chr2.4:4278778-4278797 None:intergenic 45.0%
GCCACAAAAGCTCAACTCTA+CGG + chr2.4:4278687-4278706 MS.gene85211:CDS 45.0%
! ACCGTAGAGTTGAGCTTTTG+TGG - chr2.4:4278691-4278710 None:intergenic 45.0%
! GAAGGAGGATGAGAAGTATG+AGG - chr2.4:4278797-4278816 None:intergenic 45.0%
! GCTTTTGTGGCTGGAAATGA+TGG - chr2.4:4278678-4278697 None:intergenic 45.0%
! TAGAGTTGAGCTTTTGTGGC+TGG - chr2.4:4278687-4278706 None:intergenic 45.0%
!! GAATTGTGATGGTGGTTGGT+TGG - chr2.4:4278998-4279017 None:intergenic 45.0%
!! GAGAGAATTGTGATGGTGGT+TGG - chr2.4:4279002-4279021 None:intergenic 45.0%
!! GCCATGTGTGAAAGTTGGTA+TGG - chr2.4:4279051-4279070 None:intergenic 45.0%
!!! GAATTTTGAGTTGAGGGTGG+AGG - chr2.4:4278925-4278944 None:intergenic 45.0%
AAACCCATGACCAACGGCAA+CGG + chr2.4:4278612-4278631 MS.gene85211:CDS 50.0%
CATGTTCTTCTTCAGCTCCG+TGG - chr2.4:4279141-4279160 None:intergenic 50.0%
TTCGAAGTTGACGCCGTTAC+TGG - chr2.4:4279305-4279324 None:intergenic 50.0%
! AGAAGAAGGTGCAGCAGCAA+CGG - chr2.4:4278764-4278783 None:intergenic 50.0%
! GTCACCAACGGTAACGGTAA+CGG + chr2.4:4278636-4278655 MS.gene85211:CDS 50.0%
!! AACGGTGTCACCAACGGTAA+CGG + chr2.4:4278630-4278649 MS.gene85211:CDS 50.0%
!! GGCTGGAAATGATGGGTTTG+CGG - chr2.4:4278670-4278689 None:intergenic 50.0%
!! GGTGGTTGGTTGGTAAACGA+AGG - chr2.4:4278988-4279007 None:intergenic 50.0%
!! TGGAAATGATGGGTTTGCGG+CGG - chr2.4:4278667-4278686 None:intergenic 50.0%
!!! TTTTGAGTTGAGGGTGGAGG+AGG - chr2.4:4278922-4278941 None:intergenic 50.0%
ACACCGTTGCCGTTGGTCAT+GGG - chr2.4:4278618-4278637 None:intergenic 55.0%
CTCATCCTCCTTCTCCTCCT+CGG + chr2.4:4278804-4278823 MS.gene85211:CDS 55.0%
GACACCGTTACCGTTACCGT+TGG - chr2.4:4278643-4278662 None:intergenic 55.0%
GACGTTGTGGACGGTAGGTA+GGG - chr2.4:4278722-4278741 None:intergenic 55.0%
GCTGCTGCACCTTCTTCTTC+TGG + chr2.4:4278766-4278785 MS.gene85211:CDS 55.0%
GTTGCCGTTGGTCATGGGTT+TGG - chr2.4:4278613-4278632 None:intergenic 55.0%
TACGGTGAAGGAAGGACGGT+GGG - chr2.4:4278865-4278884 None:intergenic 55.0%
TGACGTTGTGGACGGTAGGT+AGG - chr2.4:4278723-4278742 None:intergenic 55.0%
TTACGGTGAAGGAAGGACGG+TGG - chr2.4:4278866-4278885 None:intergenic 55.0%
!! AACGGCAACGGTGTCACCAA+CGG + chr2.4:4278624-4278643 MS.gene85211:CDS 55.0%
ACGGTGAAGGAAGGACGGTG+GGG - chr2.4:4278864-4278883 None:intergenic 60.0%
CCTTCTCCTCCTCGGCGTAA+CGG + chr2.4:4278812-4278831 MS.gene85211:CDS 60.0%
CTTCTCCTCCTCGGCGTAAC+GGG + chr2.4:4278813-4278832 MS.gene85211:CDS 60.0%
GACACCGTTGCCGTTGGTCA+TGG - chr2.4:4278619-4278638 None:intergenic 60.0%
GCCGCCAAACCCATGACCAA+CGG + chr2.4:4278606-4278625 MS.gene85211:CDS 60.0%
GCCGTTGGTCATGGGTTTGG+CGG - chr2.4:4278610-4278629 None:intergenic 60.0%
GGAGGAGGTGAGGTTGAGGT+AGG - chr2.4:4278904-4278923 None:intergenic 60.0%
GTTGGTCATGGGTTTGGCGG+CGG - chr2.4:4278607-4278626 None:intergenic 60.0%
GTTGTGGACGGTAGGTAGGG+CGG - chr2.4:4278719-4278738 None:intergenic 60.0%
TGAAGGAAGGACGGTGGGGA+CGG - chr2.4:4278860-4278879 None:intergenic 60.0%
TGGAGGAGGAGGTGAGGTTG+AGG - chr2.4:4278908-4278927 None:intergenic 60.0%
TTACGCCGAGGAGGAGAAGG+AGG - chr2.4:4278812-4278831 None:intergenic 60.0%
TTGGTCATGGGTTTGGCGGC+GGG - chr2.4:4278606-4278625 None:intergenic 60.0%
TTGTGGACGGTAGGTAGGGC+GGG - chr2.4:4278718-4278737 None:intergenic 60.0%
! AGAAGGTGCAGCAGCAACGG+CGG - chr2.4:4278761-4278780 None:intergenic 60.0%
! GTGGTGACGTTGTGGACGGT+AGG - chr2.4:4278727-4278746 None:intergenic 60.0%
! TGAGTTGAGGGTGGAGGAGG+AGG - chr2.4:4278919-4278938 None:intergenic 60.0%
!! GTTGGTGACACCGTTGCCGT+TGG - chr2.4:4278625-4278644 None:intergenic 60.0%
CCGTTACGCCGAGGAGGAGA+AGG - chr2.4:4278815-4278834 None:intergenic 65.0%
GACGGTGGGGACGGTAGATG+AGG - chr2.4:4278851-4278870 None:intergenic 65.0%
TGAGGGTGGAGGAGGAGGTG+AGG - chr2.4:4278914-4278933 None:intergenic 65.0%
! GGCGGTGGTGACGTTGTGGA+CGG - chr2.4:4278731-4278750 None:intergenic 65.0%
! GTACGGCGGTGGTGACGTTG+TGG - chr2.4:4278735-4278754 None:intergenic 65.0%
AAGCGGTGCCCGTTACGCCG+AGG - chr2.4:4278824-4278843 None:intergenic 70.0%
AGCAGCAACGGCGGCGGGTA+CGG - chr2.4:4278752-4278771 None:intergenic 70.0%
AGGTGCAGCAGCAACGGCGG+CGG - chr2.4:4278758-4278777 None:intergenic 70.0%
TGGACGGTAGGTAGGGCGGG+TGG - chr2.4:4278715-4278734 None:intergenic 70.0%
AGCAACGGCGGCGGGTACGG+CGG - chr2.4:4278749-4278768 None:intergenic 75.0%
CGGTGCCCGTTACGCCGAGG+AGG - chr2.4:4278821-4278840 None:intergenic 75.0%
GGTGCAGCAGCAACGGCGGC+GGG - chr2.4:4278757-4278776 None:intergenic 75.0%
! AACGGCGGCGGGTACGGCGG+TGG - chr2.4:4278746-4278765 None:intergenic 80.0%


Chromosome Type Strat End Strand Name
chr2.4 gene 4278585 4279376 4278585 ID=MS.gene85211
chr2.4 mRNA 4278585 4279376 4278585 ID=MS.gene85211.t1;Parent=MS.gene85211
chr2.4 exon 4278585 4279376 4278585 ID=MS.gene85211.t1.exon1;Parent=MS.gene85211.t1
chr2.4 CDS 4278585 4279376 4278585 ID=cds.MS.gene85211.t1;Parent=MS.gene85211.t1
Gene Sequence

>MS.gene85211

ATGGCAGATCGTGTATATCCCGCCGCCAAACCCATGACCAACGGCAACGGTGTCACCAACGGTAACGGTAACGGTGTCTCCGCCGCAAACCCATCATTTCCAGCCACAAAAGCTCAACTCTACGGTGCCACCCGCCCTACCTACCGTCCACAACGTCACCACCGCCGTACCCGCCGCCGTTGCTGCTGCACCTTCTTCTTCTGGTTCTTCCTCATACTTCTCATCCTCCTTCTCCTCCTCGGCGTAACGGGCACCGCTTTCTACCTCATCTACCGTCCCCACCGTCCTTCCTTCACCGTAACATCTCTCAAACTTTCCTACCTCAACCTCACCTCCTCCTCCACCCTCAACTCAAAATTCAACGTCAACATCACTGCAAAAAACCCTAACAAACATATCACCTTCGTTTACCAACCAACCACCATCACAATTCTCTCAAACAACATCAACATCGGTGAAGGAACCATACCAACTTTCACACATGGCAAGAAGAACACTACTCTTCTAAAATCTTCCATTTTGAGTACTGGTCAAGCATTAGAATCAGACGCTTCCACGGAGCTGAAGAAGAACATGAAGAGCAAAAACGGTTTAGCGTTGAAAGTGAAGTTGGATACAAAAGTGAAGGCTAAAGTGGGAAAAATGAAAACACCTAACGTTGGAATAAGAGTTACATGTGACGGTGTTAGAGTTCATGTTCCTGTCGGTAATAAGAAACCAGTAACGGCGTCAACTTCGAACGTTAAGTGTGATGTTGATGTTAGGTTTAAGATCTGGAAATGGACCGTTTAA
Protein sequence

>MS.gene85211.t1

MADRVYPAAKPMTNGNGVTNGNGNGVSAANPSFPATKAQLYGATRPTYRPQRHHRRTRRRCCCTFFFWFFLILLILLLLLGVTGTAFYLIYRPHRPSFTVTSLKLSYLNLTSSSTLNSKFNVNITAKNPNKHITFVYQPTTITILSNNINIGEGTIPTFTHGKKNTTLLKSSILSTGQALESDASTELKKNMKSKNGLALKVKLDTKVKAKVGKMKTPNVGIRVTCDGVRVHVPVGNKKPVTASTSNVKCDVDVRFKIWKWTV