AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene85381

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene85381.t1 MTR_2g038720 91.582 297 25 0 1 297 1 297 2.06e-179 496
MS.gene85381.t1 MTR_4g126350 43.807 331 137 16 3 291 4 327 1.11e-47 162
MS.gene85381.t1 MTR_1g016170 62.195 82 25 2 147 228 170 245 1.26e-25 103
MS.gene85381.t1 MTR_4g121110 62.000 50 19 0 159 208 25 74 3.19e-17 77.0
MS.gene85381.t1 MTR_7g112330 75.000 36 9 0 164 199 22 57 5.18e-14 68.2
MS.gene85381.t1 MTR_5g020230 44.706 85 41 1 122 200 74 158 3.01e-13 68.2
MS.gene85381.t1 MTR_1g116010 64.865 37 13 0 163 199 163 199 1.04e-11 63.9
MS.gene85381.t1 MTR_8g077510 65.789 38 13 0 163 200 124 161 7.22e-11 61.6
MS.gene85381.t1 MTR_1g006650 42.647 68 38 1 164 230 234 301 8.37e-11 62.4
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene85381.t1 AT4G26150 35.714 294 141 10 44 291 61 352 8.26e-27 107
MS.gene85381.t1 AT5G56860 34.826 201 82 9 46 197 63 263 3.54e-19 87.4
MS.gene85381.t1 AT5G49300 62.500 56 20 1 147 201 18 73 6.23e-18 79.0
MS.gene85381.t1 AT5G26930 73.171 41 11 0 161 201 23 63 1.99e-17 77.0
MS.gene85381.t1 AT3G06740 63.830 47 17 0 156 202 33 79 5.42e-15 71.2
MS.gene85381.t1 AT3G16870 68.293 41 13 0 161 201 39 79 2.27e-14 70.5
MS.gene85381.t1 AT4G16141 65.854 41 14 0 161 201 34 74 4.15e-14 70.1
MS.gene85381.t1 AT1G08010 42.857 84 47 1 147 229 203 286 1.74e-13 70.1
MS.gene85381.t1 AT1G08010 42.857 84 47 1 147 229 203 286 1.74e-13 70.1
MS.gene85381.t1 AT1G08010 42.857 84 47 1 147 229 203 286 1.74e-13 70.1
MS.gene85381.t1 AT3G50870 61.538 52 19 1 163 214 151 201 1.39e-12 67.4
MS.gene85381.t1 AT1G08000 40.698 86 47 2 115 199 171 253 1.40e-12 67.4
MS.gene85381.t1 AT1G08000 40.698 86 47 2 115 199 171 253 1.40e-12 67.4
MS.gene85381.t1 AT4G36620 59.184 49 20 0 159 207 70 118 3.95e-12 64.7
MS.gene85381.t1 AT2G18380 70.732 41 11 1 164 203 92 132 4.82e-12 64.3
MS.gene85381.t1 AT2G45050 37.500 80 49 1 152 230 167 246 1.26e-11 64.3
MS.gene85381.t1 AT3G60530 32.407 108 67 2 131 237 130 232 1.62e-11 63.5
MS.gene85381.t1 AT4G32890 36.458 96 59 2 164 257 197 292 1.96e-11 63.9

Find 58 sgRNAs with CRISPR-Local

Find 86 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TTATCTTCTGGCTTGCTTAA+TGG 0.177324 2.1:-47598340 MS.gene85381:CDS
ATCTTGTTTGATCTTGATTT+TGG 0.229038 2.1:+47599954 None:intergenic
CTACTCATGGCTTTATCTTC+TGG 0.251698 2.1:-47598352 MS.gene85381:CDS
TTTCAAACAACAACACTGTT+AGG 0.256322 2.1:-47599403 MS.gene85381:CDS
TTAATCATTCTCTTCTTTGA+TGG 0.270861 2.1:+47599530 None:intergenic
TCCTCAGTTCTTACTAGATT+TGG 0.275505 2.1:+47599443 None:intergenic
ATCAAACAAGATCTTATTCT+TGG 0.275784 2.1:-47599943 MS.gene85381:CDS
GGATCATCAAGAAGTGAAAA+AGG 0.339108 2.1:-47599627 MS.gene85381:CDS
AACTTCTTTCCCTTCACATA+TGG 0.359398 2.1:+47598541 None:intergenic
CACCATTTGCAGCGGCTGCA+AGG 0.367222 2.1:+47598587 None:intergenic
CATCTTGAGGGAAAACTTGA+TGG 0.376163 2.1:+47598392 None:intergenic
GCCCTTGCAGCCGCTGCAAA+TGG 0.402224 2.1:-47598589 MS.gene85381:CDS
CTCTGGAGAAGTGGACCAAC+TGG 0.407869 2.1:-47599347 MS.gene85381:CDS
ACCATTTGCAGCGGCTGCAA+GGG 0.414991 2.1:+47598588 None:intergenic
TTGTAACTTCTGGTGAATCA+TGG 0.418749 2.1:-47599649 MS.gene85381:CDS
CAAAGAAGAGAATGATTAAG+AGG 0.444414 2.1:-47599526 MS.gene85381:CDS
TCAAACAAGATCTTATTCTT+GGG 0.457205 2.1:-47599942 MS.gene85381:CDS
TTCAAACAACAACACTGTTA+GGG 0.464863 2.1:-47599402 MS.gene85381:CDS
GAGAATGATTAAGAGGATGA+TGG 0.470043 2.1:-47599519 MS.gene85381:CDS
TAGAGAAGAAGATGAAGAAG+TGG 0.474019 2.1:+47599997 None:intergenic
TTGAAGATTTGATAACAAGT+TGG 0.482551 2.1:-47598432 MS.gene85381:CDS
AGTTGCAAGTCAGAAGGAAA+AGG 0.484061 2.1:-47598522 MS.gene85381:CDS
CTACACCATCACTTAATATG+TGG 0.485834 2.1:+47599912 None:intergenic
GAGGATGATGGAAGATCAAA+GGG 0.487515 2.1:-47599507 MS.gene85381:CDS
TGTAACTTCTGGTGAATCAT+GGG 0.493931 2.1:-47599648 MS.gene85381:CDS
GATGCAGAATAACGATAAGT+TGG 0.493962 2.1:+47600094 None:intergenic
TTGGTCCACTTCTCCAGAGA+GGG 0.496789 2.1:+47599351 None:intergenic
ATAACGATAAGTTGGTATCA+TGG 0.500444 2.1:+47600102 None:intergenic
AACAAGTTGGAGCAACAACT+TGG 0.506773 2.1:-47598419 MS.gene85381:CDS
ACACAACTAAGACCCCTCTC+TGG 0.508622 2.1:-47599364 MS.gene85381:CDS
AATGTGCACCACAATTGAAA+AGG 0.511689 2.1:-47598486 MS.gene85381:CDS
AAAATTTAGCATTCATCAGA+AGG 0.513032 2.1:+47598313 None:intergenic
CAGCCTCCTTCACATCTTGA+GGG 0.514868 2.1:+47598380 None:intergenic
GTTGATTAGTATTGAAGAGA+TGG 0.528693 2.1:+47600023 None:intergenic
AGAGGATGATGGAAGATCAA+AGG 0.536944 2.1:-47599508 MS.gene85381:CDS
GCAGCCTCCTTCACATCTTG+AGG 0.537105 2.1:+47598379 None:intergenic
AGTCACTTTGCAATGCCTGC+GGG 0.545864 2.1:-47598636 MS.gene85381:CDS
AAGACCCCTCTCTGGAGAAG+TGG 0.553472 2.1:-47599356 MS.gene85381:CDS
GAAAGAAGTTGCAAGTCAGA+AGG 0.562695 2.1:-47598528 MS.gene85381:CDS
CATATTAAGTGATGGTGTAG+AGG 0.563231 2.1:-47599909 MS.gene85381:intron
GGCAGAGAAACCATATGTGA+AGG 0.565007 2.1:-47598551 MS.gene85381:CDS
TGCACCACAATTGAAAAGGA+AGG 0.566431 2.1:-47598482 MS.gene85381:CDS
GGAGGCTGCTATTCTACTCA+TGG 0.567161 2.1:-47598365 MS.gene85381:CDS
AAGTGGACCAACTGGTCCCA+AGG 0.591915 2.1:-47599339 MS.gene85381:intron
TGCAAATGGTGAAACTCTTG+TGG 0.595671 2.1:-47598575 MS.gene85381:CDS
AGAGAGGGGTCTTAGTTGTG+TGG 0.598153 2.1:+47599366 None:intergenic
ATCTTGAGGGAAAACTTGAT+GGG 0.607027 2.1:+47598393 None:intergenic
ATATGTACCTTGGGACCAGT+TGG 0.609719 2.1:+47599332 None:intergenic
CAGTCACTTTGCAATGCCTG+CGG 0.611660 2.1:-47598637 MS.gene85381:CDS
TCAAACCACATATTAAGTGA+TGG 0.619574 2.1:-47599917 MS.gene85381:CDS
TGGTCCACTTCTCCAGAGAG+GGG 0.626088 2.1:+47599352 None:intergenic
GCAGAGAAACCATATGTGAA+GGG 0.631828 2.1:-47598550 MS.gene85381:CDS
GCACCACAATTGAAAAGGAA+GGG 0.634758 2.1:-47598481 MS.gene85381:CDS
TTTCCCTCAAGATGTGAAGG+AGG 0.643884 2.1:-47598383 MS.gene85381:CDS
AAGAGTTTCACCATTTGCAG+CGG 0.647090 2.1:+47598579 None:intergenic
AAATGGTGAAACTCTTGTGG+TGG 0.679475 2.1:-47598572 MS.gene85381:CDS
GTTGGTCCACTTCTCCAGAG+AGG 0.682429 2.1:+47599350 None:intergenic
ACCAAATCTAGTAAGAACTG+AGG 0.700207 2.1:-47599444 MS.gene85381:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! AAATAATTTTTTTTTAATTA+TGG - chr2.1:47599488-47599507 MS.gene85381:CDS 0.0%
!!! ATATATATATAAAATTTTAA+AGG + chr2.1:47598583-47598602 None:intergenic 0.0%
!!! TATATATATAAAATTTTAAA+GGG + chr2.1:47598582-47598601 None:intergenic 0.0%
!! AGTAGTAATAAAAAAAAAAT+TGG + chr2.1:47599255-47599274 None:intergenic 10.0%
!! TCATTATTTAGATCAAAAAA+AGG + chr2.1:47598363-47598382 None:intergenic 15.0%
!! TACAAAATTCTTAACCTAAA+AGG + chr2.1:47598754-47598773 None:intergenic 20.0%
!!! TTAGAGAAATGTTTTAAAGA+TGG - chr2.1:47599193-47599212 MS.gene85381:intron 20.0%
! ATCAAACAAGATCTTATTCT+TGG - chr2.1:47598475-47598494 MS.gene85381:CDS 25.0%
! GAGTTCGAGATTAATTAATT+TGG - chr2.1:47599215-47599234 MS.gene85381:intron 25.0%
! TCAAACAAGATCTTATTCTT+GGG - chr2.1:47598476-47598495 MS.gene85381:CDS 25.0%
! TGAAAAAGGATGAAAAAGAT+TGG - chr2.1:47598805-47598824 MS.gene85381:intron 25.0%
! TTAATCATTCTCTTCTTTGA+TGG + chr2.1:47598891-47598910 None:intergenic 25.0%
!! GTTAAGAATTTTGTAACTTC+TGG - chr2.1:47598759-47598778 MS.gene85381:intron 25.0%
!! TTCATGATTAAGCTCTTTTA+TGG - chr2.1:47599635-47599654 MS.gene85381:CDS 25.0%
!! TTGAAGATTTGATAACAAGT+TGG - chr2.1:47599986-47600005 MS.gene85381:CDS 25.0%
!! TTTAGATCAAAAAAAGGCAT+AGG + chr2.1:47598357-47598376 None:intergenic 25.0%
!!! ATCTTGTTTGATCTTGATTT+TGG + chr2.1:47598467-47598486 None:intergenic 25.0%
ATATTAGATGTCAGTCTATG+AGG - chr2.1:47599329-47599348 MS.gene85381:intron 30.0%
CAAAGAAGAGAATGATTAAG+AGG - chr2.1:47598892-47598911 MS.gene85381:intron 30.0%
GAAAACTAAAGCCTCTATTA+TGG + chr2.1:47599373-47599392 None:intergenic 30.0%
GAACAAATCTTATACAAGCA+AGG + chr2.1:47599618-47599637 None:intergenic 30.0%
TCAAACCACATATTAAGTGA+TGG - chr2.1:47598501-47598520 MS.gene85381:CDS 30.0%
TTCAAACAACAACACTGTTA+GGG - chr2.1:47599016-47599035 MS.gene85381:intron 30.0%
TTGTTTCAGAACCATAATAG+AGG - chr2.1:47599359-47599378 MS.gene85381:CDS 30.0%
TTTCAAACAACAACACTGTT+AGG - chr2.1:47599015-47599034 MS.gene85381:intron 30.0%
! AGACGAGTATGAAAGTTTTA+TGG - chr2.1:47598836-47598855 MS.gene85381:intron 30.0%
! ATTAAGCTCTTTTATGGCTT+AGG - chr2.1:47599641-47599660 MS.gene85381:CDS 30.0%
! GTTGATTAGTATTGAAGAGA+TGG + chr2.1:47598398-47598417 None:intergenic 30.0%
!! CTATTATGGTTCTGAAACAA+TGG + chr2.1:47599359-47599378 None:intergenic 30.0%
AACTTCTTTCCCTTCACATA+TGG + chr2.1:47599880-47599899 None:intergenic 35.0%
AATGTGCACCACAATTGAAA+AGG - chr2.1:47599932-47599951 MS.gene85381:CDS 35.0%
ACCAAATCTAGTAAGAACTG+AGG - chr2.1:47598974-47598993 MS.gene85381:intron 35.0%
ATCTTGAGGGAAAACTTGAT+GGG + chr2.1:47600028-47600047 None:intergenic 35.0%
CTACACCATCACTTAATATG+TGG + chr2.1:47598509-47598528 None:intergenic 35.0%
GAGAATGATTAAGAGGATGA+TGG - chr2.1:47598899-47598918 MS.gene85381:intron 35.0%
GATGCAGAATAACGATAAGT+TGG + chr2.1:47598327-47598346 None:intergenic 35.0%
GGATCATCAAGAAGTGAAAA+AGG - chr2.1:47598791-47598810 MS.gene85381:intron 35.0%
TAGAGAAGAAGATGAAGAAG+TGG + chr2.1:47598424-47598443 None:intergenic 35.0%
TCCTCAGTTCTTACTAGATT+TGG + chr2.1:47598978-47598997 None:intergenic 35.0%
TGTAACTTCTGGTGAATCAT+GGG - chr2.1:47598770-47598789 MS.gene85381:intron 35.0%
TTGTAACTTCTGGTGAATCA+TGG - chr2.1:47598769-47598788 MS.gene85381:intron 35.0%
! AGATCAAAAAAAGGCATAGG+AGG + chr2.1:47598354-47598373 None:intergenic 35.0%
! CATATTAAGTGATGGTGTAG+AGG - chr2.1:47598509-47598528 MS.gene85381:CDS 35.0%
! CATGTGATTGTCATCCTTTT+AGG - chr2.1:47598737-47598756 MS.gene85381:intron 35.0%
! TTACCCTTCCTTTTCAATTG+TGG + chr2.1:47599943-47599962 None:intergenic 35.0%
!! GTATGAAAGTTTTATGGCTG+AGG - chr2.1:47598842-47598861 MS.gene85381:intron 35.0%
!! TTATCTTCTGGCTTGCTTAA+TGG - chr2.1:47600078-47600097 MS.gene85381:CDS 35.0%
AAATGGTGAAACTCTTGTGG+TGG - chr2.1:47599846-47599865 MS.gene85381:intron 40.0%
AAGAGTTTCACCATTTGCAG+CGG + chr2.1:47599842-47599861 None:intergenic 40.0%
AGTTGCAAGTCAGAAGGAAA+AGG - chr2.1:47599896-47599915 MS.gene85381:intron 40.0%
CATCTTGAGGGAAAACTTGA+TGG + chr2.1:47600029-47600048 None:intergenic 40.0%
GAAAGAAGTTGCAAGTCAGA+AGG - chr2.1:47599890-47599909 MS.gene85381:intron 40.0%
GCACCACAATTGAAAAGGAA+GGG - chr2.1:47599937-47599956 MS.gene85381:CDS 40.0%
GCAGAGAAACCATATGTGAA+GGG - chr2.1:47599868-47599887 MS.gene85381:intron 40.0%
TGCAAATGGTGAAACTCTTG+TGG - chr2.1:47599843-47599862 MS.gene85381:intron 40.0%
TGCACCACAATTGAAAAGGA+AGG - chr2.1:47599936-47599955 MS.gene85381:CDS 40.0%
! AACAAGTTGGAGCAACAACT+TGG - chr2.1:47599999-47600018 MS.gene85381:CDS 40.0%
! AGTTTTCCCTCAAGATGTGA+AGG - chr2.1:47600032-47600051 MS.gene85381:CDS 40.0%
!! AGAGGATGATGGAAGATCAA+AGG - chr2.1:47598910-47598929 MS.gene85381:intron 40.0%
!! CTACTCATGGCTTTATCTTC+TGG - chr2.1:47600066-47600085 MS.gene85381:CDS 40.0%
!! GAGGATGATGGAAGATCAAA+GGG - chr2.1:47598911-47598930 MS.gene85381:intron 40.0%
ATATGTACCTTGGGACCAGT+TGG + chr2.1:47599089-47599108 None:intergenic 45.0%
CCCAAGGTACATATTGCTCA+CGG - chr2.1:47599095-47599114 MS.gene85381:intron 45.0%
CCGTGAGCAATATGTACCTT+GGG + chr2.1:47599098-47599117 None:intergenic 45.0%
GGCAGAGAAACCATATGTGA+AGG - chr2.1:47599867-47599886 MS.gene85381:intron 45.0%
TCCGTGAGCAATATGTACCT+TGG + chr2.1:47599099-47599118 None:intergenic 45.0%
TTTCCCTCAAGATGTGAAGG+AGG - chr2.1:47600035-47600054 MS.gene85381:CDS 45.0%
! TTTTCTTTGTCGAATCCCGC+AGG + chr2.1:47599800-47599819 None:intergenic 45.0%
!!! ATATATATAAAATTTTAAAG+GGG + chr2.1:47598581-47598600 None:intergenic 5.0%
!!! GAAAAAAATATTATTTTAAA+AGG + chr2.1:47599127-47599146 None:intergenic 5.0%
ACACAACTAAGACCCCTCTC+TGG - chr2.1:47599054-47599073 MS.gene85381:intron 50.0%
AGAGAGGGGTCTTAGTTGTG+TGG + chr2.1:47599055-47599074 None:intergenic 50.0%
CAGCCTCCTTCACATCTTGA+GGG + chr2.1:47600041-47600060 None:intergenic 50.0%
GGAGGCTGCTATTCTACTCA+TGG - chr2.1:47600053-47600072 MS.gene85381:CDS 50.0%
TTGGTCCACTTCTCCAGAGA+GGG + chr2.1:47599070-47599089 None:intergenic 50.0%
! AGTCACTTTGCAATGCCTGC+GGG - chr2.1:47599782-47599801 MS.gene85381:intron 50.0%
! CAGTCACTTTGCAATGCCTG+CGG - chr2.1:47599781-47599800 MS.gene85381:intron 50.0%
AAGTGGACCAACTGGTCCCA+AGG - chr2.1:47599079-47599098 MS.gene85381:intron 55.0%
ACCATTTGCAGCGGCTGCAA+GGG + chr2.1:47599833-47599852 None:intergenic 55.0%
GCAGCCTCCTTCACATCTTG+AGG + chr2.1:47600042-47600061 None:intergenic 55.0%
GTTGGTCCACTTCTCCAGAG+AGG + chr2.1:47599071-47599090 None:intergenic 55.0%
TGGTCCACTTCTCCAGAGAG+GGG + chr2.1:47599069-47599088 None:intergenic 55.0%
! AAGACCCCTCTCTGGAGAAG+TGG - chr2.1:47599062-47599081 MS.gene85381:intron 55.0%
! CTCTGGAGAAGTGGACCAAC+TGG - chr2.1:47599071-47599090 MS.gene85381:intron 55.0%
CACCATTTGCAGCGGCTGCA+AGG + chr2.1:47599834-47599853 None:intergenic 60.0%
GCCCTTGCAGCCGCTGCAAA+TGG - chr2.1:47599829-47599848 MS.gene85381:intron 65.0%


Chromosome Type Strat End Strand Name
chr2.1 gene 47598318 47600122 47598318 ID=MS.gene85381
chr2.1 mRNA 47598318 47600122 47598318 ID=MS.gene85381.t1;Parent=MS.gene85381
chr2.1 exon 47599910 47600122 47599910 ID=MS.gene85381.t1.exon1;Parent=MS.gene85381.t1
chr2.1 CDS 47599910 47600122 47599910 ID=cds.MS.gene85381.t1;Parent=MS.gene85381.t1
chr2.1 exon 47599340 47599681 47599340 ID=MS.gene85381.t1.exon2;Parent=MS.gene85381.t1
chr2.1 CDS 47599340 47599681 47599340 ID=cds.MS.gene85381.t1;Parent=MS.gene85381.t1
chr2.1 exon 47598318 47598656 47598318 ID=MS.gene85381.t1.exon3;Parent=MS.gene85381.t1
chr2.1 CDS 47598318 47598656 47598318 ID=cds.MS.gene85381.t1;Parent=MS.gene85381.t1
Gene Sequence

>MS.gene85381

ATGATACCAACTTATCGTTATTCTGCATCTTCTCCTCCTATGCCTTTTTTTGATCTAAATAATGAAGATCACAGTCACCATCTCTTCAATACTAATCAACAAGCCACTTCTTCATCTTCTTCTCTATCATACTCTATACTCTTCAACCAAAATCAAGATCAAACAAGATCTTATTCTTGGGAGTCAAACCACATATTAAGTGATGGTGTAGAGGTAACATATACATATAGACATATTATATTATTTAGTATTAAGTAGCACCCCTTTAAAATTTTATATATATATCTTTGCTTTAATCAATTTGATCATGCATAAGCTTATTTATATATTTTTTGATTTATTTATTTCTGTTAAAAAAAAGCTTATTGTTTTAATATGTAGAAATTTATCTTGCTGTTTCAAAAAAAAAATTGATCAAGCATGTGATTGTCATCCTTTTAGGTTAAGAATTTTGTAACTTCTGGTGAATCATGGGATCATCAAGAAGTGAAAAAGGATGAAAAAGATTGGAAGAAAGAAGACGAGTATGAAAGTTTTATGGCTGAGGATAGAACTTCAATGAAGTTGATGCCATCAAAGAAGAGAATGATTAAGAGGATGATGGAAGATCAAAGGGCTTCTGAGCAAGAATTTGAGAAACAAATTAAGCAATTATCACCAAATCTAGTAAGAACTGAGGATAGTAGCAACAACAATTTTTCAAACAACAACACTGTTAGGGTTTGTACTGACTGCCACACAACTAAGACCCCTCTCTGGAGAAGTGGACCAACTGGTCCCAAGGTACATATTGCTCACGGATTCTTCCTTTTAAAATAATATTTTTTTCATGATAAATAATTTACTTGTTACATTGTGCTTCTAATATGAACATTTTAGAGAAATGTTTTAAAGATGGAGTTCGAGATTAATTAATTTGGTATAACAGATTTATCCAATTTTTTTTTTATTACTACTGAGAATTTTTTTTAGTGTTATATCAGATATCAAAATTCAAATGAAAACTGTTGTATATTAGATGTCAGTCTATGAGGTCTACCATTGTTTCAGAACCATAATAGAGGCTTTAGTTTTCATATGTTGATCTCGTGACTATTTTAAACATATTAACAACAATAACAAATATATTAAGTATTTCAATGTACTTTGAAGCACTACTTTAATGATTGTAAATAATTTTTTTTTAATTATGGTTGAAAATCTATTATGTTGCAAATAATTACATCAAATACTACTGATATATACAACTTCTACGACAAACTCTTGTATCGCGACATCGATATATAGATTAGATAAACCTTGCTTGTATAAGATTTGTTCATGATTAAGCTCTTTTATGGCTTAGGAACTCATCTTCGATACAACTTTGCATTAACAATGCGAGTAGAATTATTTCGAAAAAATAAATTATGCGATTTAAAATTTTAAACTAATTGTTGAAAAATATAAATATGTTTTTGTGACAGTCACTTTGCAATGCCTGCGGGATTCGACAAAGAAAAGCGAGACGCGCCCTTGCAGCCGCTGCAAATGGTGAAACTCTTGTGGTGGCAGAGAAACCATATGTGAAGGGAAAGAAGTTGCAAGTCAGAAGGAAAAGGTTAAAAACTGATCAATGTGCACCACAATTGAAAAGGAAGGGTAAAAGTGAAAACAAGTGTAATAATTTTGAAGATTTGATAACAAGTTGGAGCAACAACTTGGCTTCCCATCAAGTTTTCCCTCAAGATGTGAAGGAGGCTGCTATTCTACTCATGGCTTTATCTTCTGGCTTGCTTAATGGTTTTCCTTCTGATGAATGCTAA
Protein sequence

>MS.gene85381.t1

MIPTYRYSASSPPMPFFDLNNEDHSHHLFNTNQQATSSSSSLSYSILFNQNQDQTRSYSWESNHILSDGVEVKNFVTSGESWDHQEVKKDEKDWKKEDEYESFMAEDRTSMKLMPSKKRMIKRMMEDQRASEQEFEKQIKQLSPNLVRTEDSSNNNFSNNNTVRVCTDCHTTKTPLWRSGPTGPKSLCNACGIRQRKARRALAAAANGETLVVAEKPYVKGKKLQVRRKRLKTDQCAPQLKRKGKSENKCNNFEDLITSWSNNLASHQVFPQDVKEAAILLMALSSGLLNGFPSDEC