Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene85487.t1 | XP_003598669.1 | 85.7 | 133 | 14 | 1 | 13 | 145 | 6 | 133 | 9.20E-52 | 213 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene85487.t1 | G7IW31 | 85.7 | 133 | 14 | 1 | 13 | 145 | 6 | 133 | 6.6e-52 | 213.0 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene04915 | MS.gene85487 | 0.805647 | 1.21E-49 | -1.69E-46 |
| MS.gene04917 | MS.gene85487 | 0.801398 | 9.18E-49 | -1.69E-46 |
| MS.gene050317 | MS.gene85487 | 0.827777 | 1.30E-54 | -1.69E-46 |
| MS.gene053381 | MS.gene85487 | 0.80323 | 3.85E-49 | -1.69E-46 |
| MS.gene056752 | MS.gene85487 | 0.801314 | 9.55E-49 | -1.69E-46 |
| MS.gene056849 | MS.gene85487 | 0.81212 | 4.97E-51 | -1.69E-46 |
| MS.gene059050 | MS.gene85487 | 0.823481 | 1.35E-53 | -1.69E-46 |
| MS.gene060763 | MS.gene85487 | 0.816637 | 4.99E-52 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene85487.t1 | MTR_3g019060 | 91.729 | 133 | 6 | 1 | 13 | 145 | 6 | 133 | 1.45e-86 | 249 |
| MS.gene85487.t1 | MTR_8g085310 | 32.584 | 178 | 78 | 4 | 2 | 145 | 540 | 709 | 1.38e-22 | 92.8 |
| MS.gene85487.t1 | MTR_3g053640 | 36.735 | 147 | 89 | 2 | 2 | 145 | 288 | 433 | 1.00e-21 | 90.1 |
| MS.gene85487.t1 | MTR_4g092300 | 36.747 | 166 | 83 | 6 | 2 | 145 | 662 | 827 | 1.09e-21 | 90.5 |
| MS.gene85487.t1 | MTR_4g051480 | 36.747 | 166 | 83 | 6 | 2 | 145 | 680 | 845 | 1.16e-21 | 90.1 |
| MS.gene85487.t1 | MTR_1g094760 | 66.667 | 51 | 17 | 0 | 95 | 145 | 652 | 702 | 6.42e-19 | 82.4 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 20 sgRNAs with CRISPR-Local
Find 23 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| GGGATGGTTAGCTAGGCTTT+AGG | 0.376206 | 3.4:+12568165 | MS.gene85487:CDS |
| TGATTTCATATAACTTGTAA+TGG | 0.424534 | 3.4:-12567763 | None:intergenic |
| CAAGAATCAACACAAATAGA+TGG | 0.434051 | 3.4:+12567824 | MS.gene85487:CDS |
| ACACTCAGTAATTGAAGAAC+TGG | 0.444867 | 3.4:-12568023 | None:intergenic |
| AGAAACATTTCCCTCCAATC+AGG | 0.460479 | 3.4:-12568118 | None:intergenic |
| TTCTTGCTATGCCTAGTCAT+AGG | 0.475426 | 3.4:+12568137 | MS.gene85487:CDS |
| ATGCCTAGTCATAGGAGAAA+GGG | 0.517168 | 3.4:+12568145 | MS.gene85487:CDS |
| AGAACTGGCATTTATACTCC+TGG | 0.518145 | 3.4:-12568008 | None:intergenic |
| GAAATCAGAAGTATTGCAGC+TGG | 0.533371 | 3.4:+12567779 | MS.gene85487:CDS |
| AAAAGGAGAGGTTTAAGTCC+AGG | 0.539612 | 3.4:+12567990 | MS.gene85487:CDS |
| ATTTATGGATCCTGATTGGA+GGG | 0.542617 | 3.4:+12568108 | MS.gene85487:CDS |
| CATCCCTTTCTCCTATGACT+AGG | 0.543106 | 3.4:-12568148 | None:intergenic |
| TATGCCTAGTCATAGGAGAA+AGG | 0.549487 | 3.4:+12568144 | MS.gene85487:CDS |
| GGAGAAAGGGATGGTTAGCT+AGG | 0.552786 | 3.4:+12568158 | MS.gene85487:CDS |
| TGGTAAATGCACTAGTAGCA+TGG | 0.553678 | 3.4:+12567927 | MS.gene85487:CDS |
| AATTTATGGATCCTGATTGG+AGG | 0.587329 | 3.4:+12568107 | MS.gene85487:CDS |
| TTATTCTAGCTGAAAAGGAG+AGG | 0.593244 | 3.4:+12567978 | MS.gene85487:CDS |
| GACTGCTCTTGACAAAATTG+AGG | 0.595995 | 3.4:+12568048 | MS.gene85487:CDS |
| AGCATGGACTCAAGCATCTG+AGG | 0.606559 | 3.4:+12567943 | MS.gene85487:CDS |
| CTAGTCATAGGAGAAAGGGA+TGG | 0.706723 | 3.4:+12568149 | MS.gene85487:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | AGAATATTCAAAAAATGCAT+TGG | + | chr3.4:12567907-12567926 | MS.gene85487:CDS | 20.0% |
| !!! | TGATTTCATATAACTTGTAA+TGG | - | chr3.4:12567766-12567785 | None:intergenic | 20.0% |
| ! | AAAAATTTATGGATCCTGAT+TGG | + | chr3.4:12568104-12568123 | MS.gene85487:CDS | 25.0% |
| ! | GAAAGCTTGTGAAAAATTTA+TGG | + | chr3.4:12568093-12568112 | MS.gene85487:CDS | 25.0% |
| CAAGAATCAACACAAATAGA+TGG | + | chr3.4:12567824-12567843 | MS.gene85487:CDS | 30.0% | |
| !! | AGCTTTTATTCTAGCTGAAA+AGG | + | chr3.4:12567973-12567992 | MS.gene85487:CDS | 30.0% |
| AATTTATGGATCCTGATTGG+AGG | + | chr3.4:12568107-12568126 | MS.gene85487:CDS | 35.0% | |
| ACACTCAGTAATTGAAGAAC+TGG | - | chr3.4:12568026-12568045 | None:intergenic | 35.0% | |
| ATTTATGGATCCTGATTGGA+GGG | + | chr3.4:12568108-12568127 | MS.gene85487:CDS | 35.0% | |
| ! | TTATTCTAGCTGAAAAGGAG+AGG | + | chr3.4:12567978-12567997 | MS.gene85487:CDS | 35.0% |
| AAAAGGAGAGGTTTAAGTCC+AGG | + | chr3.4:12567990-12568009 | MS.gene85487:CDS | 40.0% | |
| AGAAACATTTCCCTCCAATC+AGG | - | chr3.4:12568121-12568140 | None:intergenic | 40.0% | |
| AGAACTGGCATTTATACTCC+TGG | - | chr3.4:12568011-12568030 | None:intergenic | 40.0% | |
| ATGCCTAGTCATAGGAGAAA+GGG | + | chr3.4:12568145-12568164 | MS.gene85487:CDS | 40.0% | |
| GACTGCTCTTGACAAAATTG+AGG | + | chr3.4:12568048-12568067 | MS.gene85487:CDS | 40.0% | |
| TATGCCTAGTCATAGGAGAA+AGG | + | chr3.4:12568144-12568163 | MS.gene85487:CDS | 40.0% | |
| TGGTAAATGCACTAGTAGCA+TGG | + | chr3.4:12567927-12567946 | MS.gene85487:CDS | 40.0% | |
| ! | TTCTTGCTATGCCTAGTCAT+AGG | + | chr3.4:12568137-12568156 | MS.gene85487:CDS | 40.0% |
| !! | GAAATCAGAAGTATTGCAGC+TGG | + | chr3.4:12567779-12567798 | MS.gene85487:CDS | 40.0% |
| CATCCCTTTCTCCTATGACT+AGG | - | chr3.4:12568151-12568170 | None:intergenic | 45.0% | |
| CTAGTCATAGGAGAAAGGGA+TGG | + | chr3.4:12568149-12568168 | MS.gene85487:CDS | 45.0% | |
| AGCATGGACTCAAGCATCTG+AGG | + | chr3.4:12567943-12567962 | MS.gene85487:CDS | 50.0% | |
| ! | GGAGAAAGGGATGGTTAGCT+AGG | + | chr3.4:12568158-12568177 | MS.gene85487:CDS | 50.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr3.4 | gene | 12567749 | 12568186 | 12567749 | ID=MS.gene85487 |
| chr3.4 | mRNA | 12567749 | 12568186 | 12567749 | ID=MS.gene85487.t1;Parent=MS.gene85487 |
| chr3.4 | exon | 12567749 | 12568186 | 12567749 | ID=MS.gene85487.t1.exon1;Parent=MS.gene85487.t1 |
| chr3.4 | CDS | 12567749 | 12568186 | 12567749 | ID=cds.MS.gene85487.t1;Parent=MS.gene85487.t1 |
| Gene Sequence |
| Protein sequence |
