AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene85554

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene85554.t1 MTR_1g105785 73.904 479 66 5 20 446 95 566 0.0 706
MS.gene85554.t1 MTR_7g051400 74.943 435 66 6 20 440 95 500 0.0 607
MS.gene85554.t1 MTR_7g110100 53.604 444 171 7 20 443 95 523 9.12e-156 451
MS.gene85554.t1 MTR_3g452170 43.953 430 161 11 20 443 95 450 2.16e-102 313
MS.gene85554.t1 MTR_4g032565 49.291 282 126 4 179 443 2 283 2.01e-88 271
MS.gene85554.t1 MTR_4g032585 48.227 282 129 3 179 443 2 283 2.42e-87 268
MS.gene85554.t1 MTR_1g492690 40.533 338 155 5 108 436 1 301 2.48e-80 253
MS.gene85554.t1 MTR_0048s0080 44.649 271 117 6 63 316 4 258 3.75e-70 226
MS.gene85554.t1 MTR_1g052715 47.423 194 93 3 20 206 54 245 4.43e-52 178
MS.gene85554.t1 MTR_7g451880 40.000 205 115 2 20 218 96 298 1.04e-44 159
MS.gene85554.t1 MTR_5g030110 40.933 193 106 2 20 206 95 285 1.36e-43 155
MS.gene85554.t1 MTR_2g007410 40.933 193 106 2 20 206 95 285 1.36e-43 155
MS.gene85554.t1 MTR_7g024060 40.933 193 106 2 20 206 95 285 1.40e-43 155
MS.gene85554.t1 MTR_6g087820 40.933 193 106 2 20 206 95 285 1.40e-43 155
MS.gene85554.t1 MTR_5g081190 40.933 193 106 2 20 206 95 285 1.40e-43 155
MS.gene85554.t1 MTR_5g091480 40.933 193 106 2 20 206 95 285 1.40e-43 155
MS.gene85554.t1 MTR_5g056290 40.933 193 106 2 20 206 95 285 1.40e-43 155
MS.gene85554.t1 MTR_3g031190 40.933 193 106 2 20 206 95 285 1.40e-43 155
MS.gene85554.t1 MTR_2g005490 40.933 193 106 2 20 206 95 285 1.40e-43 155
MS.gene85554.t1 MTR_2g010570 40.933 193 106 2 20 206 95 285 1.40e-43 155
MS.gene85554.t1 MTR_8g446640 40.933 193 106 2 20 206 76 266 1.69e-43 155
MS.gene85554.t1 MTR_3g046650 51.007 149 50 1 163 311 77 202 1.97e-43 153
MS.gene85554.t1 MTR_4g058660 39.024 205 117 2 20 218 95 297 2.62e-43 155
MS.gene85554.t1 MTR_2g010270 37.850 214 116 3 20 218 76 287 7.69e-42 150
MS.gene85554.t1 MTR_7g074180 39.062 192 109 2 21 206 96 285 1.14e-41 150
MS.gene85554.t1 MTR_5g057125 35.321 218 122 2 128 340 4 207 8.45e-39 142
MS.gene85554.t1 MTR_1g023370 38.860 193 91 3 20 206 95 266 7.29e-36 134
MS.gene85554.t1 MTR_1g011490 37.306 193 104 3 20 206 95 276 1.83e-35 134
MS.gene85554.t1 MTR_8g064300 40.887 203 97 5 256 446 120 311 3.98e-34 130
MS.gene85554.t1 MTR_5g045850 38.860 193 92 4 20 206 80 252 4.06e-34 129
MS.gene85554.t1 MTR_8g005940 37.968 187 87 3 20 206 95 252 8.54e-34 129
MS.gene85554.t1 MTR_5g019960 43.709 151 75 4 20 163 101 248 2.65e-33 126
MS.gene85554.t1 MTR_3g108500 40.107 187 77 4 258 423 20 192 4.50e-33 125
MS.gene85554.t1 MTR_3g015660 40.000 150 82 2 20 163 95 242 4.17e-32 122
MS.gene85554.t1 MTR_5g093790 40.500 200 96 5 259 446 197 385 2.98e-31 124
MS.gene85554.t1 MTR_2g048560 27.941 340 222 7 20 340 95 430 5.76e-30 122
MS.gene85554.t1 MTR_1g115520 39.216 153 57 2 208 354 5 127 2.91e-29 112
MS.gene85554.t1 MTR_5g012180 32.340 235 119 7 5 218 20 235 2.44e-28 113
MS.gene85554.t1 MTR_0105s0080 41.844 141 74 2 23 157 54 192 4.43e-28 112
MS.gene85554.t1 MTR_4g060433 29.730 259 119 4 186 444 9 204 5.50e-27 107
MS.gene85554.t1 MTR_8g104590 30.159 252 107 7 193 444 5 187 1.74e-26 105
MS.gene85554.t1 MTR_4g036240 27.246 334 228 8 20 340 15 346 1.81e-26 111
MS.gene85554.t1 MTR_8g062030 28.198 344 218 12 20 342 95 430 3.58e-26 111
MS.gene85554.t1 MTR_3g056270 27.545 334 227 8 20 340 254 585 4.50e-26 111
MS.gene85554.t1 MTR_4g036240 27.246 334 228 8 20 340 95 426 4.69e-26 111
MS.gene85554.t1 MTR_1g051235 66.667 78 25 1 20 96 95 172 5.58e-26 103
MS.gene85554.t1 MTR_8g465040 63.636 77 28 0 20 96 95 171 1.77e-25 102
MS.gene85554.t1 MTR_2g040680 44.231 156 59 4 290 445 117 244 2.75e-25 104
MS.gene85554.t1 MTR_2g040680 70.213 47 13 1 164 210 61 106 1.23e-12 67.8
MS.gene85554.t1 MTR_6g445520 25.779 353 221 9 76 402 11 348 1.51e-23 102
MS.gene85554.t1 MTR_1g082860 39.007 141 69 3 20 154 95 224 2.82e-23 99.4
MS.gene85554.t1 MTR_6g024030 40.800 125 63 3 46 161 38 160 2.90e-23 98.6
MS.gene85554.t1 MTR_4g014890 24.924 329 214 10 16 317 116 438 1.18e-22 100
MS.gene85554.t1 MTR_3g055660 25.000 324 210 10 16 312 116 433 3.35e-22 99.8
MS.gene85554.t1 MTR_4g036240 28.926 242 161 5 108 340 44 283 7.48e-22 97.4
MS.gene85554.t1 MTR_5g078250 27.551 294 194 8 62 340 138 427 8.11e-21 95.1
MS.gene85554.t1 MTR_7g045450 30.337 178 101 3 20 191 95 255 9.92e-21 91.7
MS.gene85554.t1 MTR_6g073570 30.337 178 101 3 20 191 95 255 9.92e-21 91.7
MS.gene85554.t1 MTR_6g059860 22.340 470 286 15 16 446 110 539 1.46e-20 94.7
MS.gene85554.t1 MTR_5g071020 30.131 229 97 7 5 218 20 200 2.35e-20 90.1
MS.gene85554.t1 MTR_6g465570 45.455 77 42 0 20 96 91 167 7.17e-19 84.3
MS.gene85554.t1 MTR_3g036220 29.897 194 95 4 33 218 61 221 1.61e-18 85.1
MS.gene85554.t1 MTR_7g051840 53.247 77 33 1 20 96 52 125 6.02e-18 80.1
MS.gene85554.t1 MTR_2g008650 23.964 338 238 9 20 340 97 432 5.48e-17 83.6
MS.gene85554.t1 MTR_8g479260 23.964 338 238 9 20 340 97 432 5.53e-17 83.6
MS.gene85554.t1 MTR_2g450060 22.956 318 217 8 20 312 95 409 7.07e-17 83.6
MS.gene85554.t1 MTR_5g004860 23.423 333 220 12 8 312 80 405 5.03e-16 80.5
MS.gene85554.t1 MTR_3g007260 42.574 101 49 3 42 136 77 174 6.18e-16 75.9
MS.gene85554.t1 MTR_4g085760 23.926 326 215 10 20 317 98 418 6.96e-16 80.1
MS.gene85554.t1 MTR_2g019040 35.294 136 72 3 77 206 4 129 8.53e-16 77.0
MS.gene85554.t1 MTR_3g068135 37.037 108 55 2 156 263 35 129 3.26e-14 71.6
MS.gene85554.t1 MTR_0003s0290 24.272 309 206 12 20 312 59 355 1.79e-13 72.4
MS.gene85554.t1 MTR_2g435900 38.710 93 49 2 45 131 22 112 2.21e-13 66.6
MS.gene85554.t1 MTR_3g449160 35.514 107 59 3 33 131 8 112 2.65e-13 66.6
MS.gene85554.t1 MTR_6g464080 22.193 374 241 13 38 379 111 466 5.40e-13 71.2
MS.gene85554.t1 MTR_4g017450 61.364 44 17 0 20 63 94 137 3.18e-12 64.3
MS.gene85554.t1 MTR_5g053590 27.152 302 170 11 20 288 92 376 7.50e-12 67.8
MS.gene85554.t1 MTR_7g096440 30.081 123 78 2 20 136 48 168 8.80e-12 65.9
MS.gene85554.t1 MTR_5g041170 22.848 302 195 12 43 312 17 312 1.43e-11 66.6
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene85554.t1 AT1G52950 27.011 348 216 9 20 336 94 434 6.43e-22 99.0
MS.gene85554.t1 AT2G05642 24.658 438 257 16 66 443 104 528 3.94e-18 87.0

Find 78 sgRNAs with CRISPR-Local

Find 137 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CCATTACATCCAGATGTTAT+TGG 0.146135 1.3:-54451861 MS.gene85554:intron
GCTTCCAATATTTCATTAAA+AGG 0.161805 1.3:+54452026 None:intergenic
AGAACTGTGTCTTGTCTATA+TGG 0.228497 1.3:+54452096 None:intergenic
TGGCAAGCAAATGCTATTTA+AGG 0.234899 1.3:-54450595 MS.gene85554:CDS
AGCTTCAACTGTTGTCTCTT+TGG 0.254275 1.3:-54451294 MS.gene85554:CDS
TTGAAACGTTACGGTCAAAA+AGG 0.288043 1.3:+54450818 None:intergenic
TTCTTTGGCTCAATCTTAAC+AGG 0.295065 1.3:+54449995 None:intergenic
CAACAGTTGAAGCTGTATTC+TGG 0.308672 1.3:+54451303 None:intergenic
ACGCCTTGCAGCAAACTTAC+TGG 0.314877 1.3:-54450106 MS.gene85554:CDS
TTGAAGGAAAAGGATGTAAA+TGG 0.321200 1.3:-54451816 MS.gene85554:CDS
TTACTTAGATTTGAATTCAA+TGG 0.391956 1.3:+54451465 None:intergenic
CGCAAGGTTAACCACGGTAT+TGG 0.392634 1.3:+54450183 None:intergenic
TGGTTGCTGACCAGACTTGA+TGG 0.396023 1.3:-54451356 MS.gene85554:intron
CTTCCAATATTTCATTAAAA+GGG 0.412361 1.3:+54452027 None:intergenic
GAGAGATGAATTGAAGGAAA+AGG 0.418720 1.3:-54451826 MS.gene85554:CDS
ATTATTCTTGCTACAATATG+TGG 0.423236 1.3:-54451126 MS.gene85554:CDS
TTAACAGGTTTGAGCTTGAC+AGG 0.433422 1.3:+54450010 None:intergenic
ATGGTAATCTACTTCACAAT+TGG 0.438391 1.3:-54450560 MS.gene85554:CDS
ATGAAATATTGGAAGCAGAC+AGG 0.438447 1.3:-54452019 MS.gene85554:CDS
GGATAATCAGAGGAATAGTT+TGG 0.440890 1.3:+54450639 None:intergenic
TTGACCCCGTCTGCTGATGC+TGG 0.443597 1.3:-54450052 MS.gene85554:CDS
CGTCGAGAGAGATGAATTGA+AGG 0.445448 1.3:-54451832 MS.gene85554:CDS
GGGAGAGTGAGAACAGTTGC+AGG 0.450758 1.3:-54451054 MS.gene85554:CDS
GGATCGAATCAGAATACAAT+TGG 0.451814 1.3:-54451104 MS.gene85554:CDS
GACCATTGAAGACTTGATAG+AGG 0.462760 1.3:-54451243 MS.gene85554:CDS
GTGGAAAGTGTAACACTAGA+AGG 0.463934 1.3:-54451020 MS.gene85554:CDS
GATTTGAATTCAATGGCCTC+TGG 0.465210 1.3:+54451472 None:intergenic
GACAGGGTGGAAAAGATTAT+CGG 0.488328 1.3:-54452002 MS.gene85554:intron
AAAACAACGACGGGTGATGA+TGG 0.494382 1.3:+54451586 None:intergenic
TCTTAATCAAGTCATGGAAA+AGG 0.495412 1.3:-54452344 MS.gene85554:intron
CTGCGCACTTTGTACATGCC+TGG 0.506383 1.3:+54451076 None:intergenic
AACCAGCACAAACATTCTCC+AGG 0.510733 1.3:+54450209 None:intergenic
GCAAATGCTATTTAAGGTAG+AGG 0.511833 1.3:-54450589 MS.gene85554:CDS
CATCTCCAGCATCAGCAGAC+GGG 0.519606 1.3:+54450047 None:intergenic
AGCCTCTATCAAGTCTTCAA+TGG 0.521193 1.3:+54451241 None:intergenic
TGCATTTCTTAATCAAGTCA+TGG 0.523048 1.3:-54452350 MS.gene85554:CDS
CAGGCATGTACAAAGTGCGC+AGG 0.537094 1.3:-54451075 MS.gene85554:CDS
ACCCTGGAGAATGTTTGTGC+TGG 0.539636 1.3:-54450211 MS.gene85554:CDS
CAAATTGCATGTTCAAGTGA+TGG 0.540470 1.3:-54450866 MS.gene85554:CDS
ATGATGGATCATGAGCAGTC+AGG 0.545330 1.3:+54451602 None:intergenic
GCTAATTGATCTCACACTGC+AGG 0.547529 1.3:-54451781 MS.gene85554:CDS
CAAGAGAACAACTGCTGATG+AGG 0.550096 1.3:-54450523 MS.gene85554:CDS
TGTTGGATCAAAACAACGAC+GGG 0.552535 1.3:+54451577 None:intergenic
TGAAATATTGGAAGCAGACA+GGG 0.559589 1.3:-54452018 MS.gene85554:CDS
GAAAAGATTGCACACCTCAT+CGG 0.560657 1.3:+54450335 None:intergenic
GCAGCAAACTTACTGGAAAG+AGG 0.561926 1.3:-54450099 MS.gene85554:CDS
ACAAAATCGAAATGATTCAA+AGG 0.562140 1.3:+54452050 None:intergenic
TTATTCTTGCTACAATATGT+GGG 0.564659 1.3:-54451125 MS.gene85554:CDS
GTTGCAGGTCGTCAGTATTG+TGG 0.567194 1.3:-54451039 MS.gene85554:CDS
TGTAGAAGAGGGATCTACAT+AGG 0.581422 1.3:-54452130 MS.gene85554:intron
ACATCTCCAGCATCAGCAGA+CGG 0.585865 1.3:+54450046 None:intergenic
TTTCCAGTAAGTTTGCTGCA+AGG 0.586359 1.3:+54450103 None:intergenic
CAATGACAAGGAGCAAGAAG+TGG 0.587798 1.3:-54450134 MS.gene85554:CDS
AGAATACAATTGGTATTACC+AGG 0.592803 1.3:-54451094 MS.gene85554:CDS
GGGACTAAACTGTTGCTGGA+TGG 0.593752 1.3:-54451502 MS.gene85554:CDS
ACAAAAGAGTCGAGATCTGA+CGG 0.594668 1.3:+54450618 None:intergenic
ATCTCCAGCATCAGCAGACG+GGG 0.596529 1.3:+54450048 None:intergenic
TGCTGGTTCATCCAATACCG+TGG 0.604250 1.3:-54450194 MS.gene85554:CDS
AAAAGGATGTAAATGGTCGT+AGG 0.607366 1.3:-54451809 MS.gene85554:CDS
AGACTTGATAGAGGCTTCTG+AGG 0.614987 1.3:-54451234 MS.gene85554:intron
CTTGATTAAGAAATGCACAT+AGG 0.615825 1.3:+54452356 None:intergenic
AGGCATGTACAAAGTGCGCA+GGG 0.628961 1.3:-54451074 MS.gene85554:CDS
CAATACCGTGGTTAACCTTG+CGG 0.630384 1.3:-54450182 MS.gene85554:CDS
ATAACTACCATAAAATTGAG+AGG 0.637237 1.3:+54451733 None:intergenic
CTAGAAGGAATGCAGTGCCA+AGG 0.641839 1.3:-54451005 MS.gene85554:intron
GAGATCTGACGGATAATCAG+AGG 0.642290 1.3:+54450629 None:intergenic
GCTGGATGGTGACTTGCCAG+AGG 0.650807 1.3:-54451488 MS.gene85554:CDS
TTTAAGGTAGAGGTCAGTGA+TGG 0.652125 1.3:-54450579 MS.gene85554:CDS
CATGATCGAGAGCATCCGCA+AGG 0.662494 1.3:+54450167 None:intergenic
TTGTTGGATCAAAACAACGA+CGG 0.663289 1.3:+54451576 None:intergenic
ACCAGCACAAACATTCTCCA+GGG 0.666454 1.3:+54450210 None:intergenic
TTAAGAAATGCACATAGGAG+AGG 0.672493 1.3:+54452361 None:intergenic
GTTGACATTGAATGCCGATG+AGG 0.685320 1.3:-54450349 MS.gene85554:intron
AATATTGGAAGCAGACAGGG+TGG 0.687117 1.3:-54452015 MS.gene85554:CDS
AGCATCCGCAAGGTTAACCA+CGG 0.695722 1.3:+54450177 None:intergenic
TTGCTGACCAGACTTGATGG+TGG 0.698383 1.3:-54451353 MS.gene85554:intron
TGTGACTAACAACAATGACA+AGG 0.705567 1.3:-54450146 MS.gene85554:CDS
GTTCAAGTGATGGACAACAC+AGG 0.714002 1.3:-54450856 MS.gene85554:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TTAAAAAAAATCAATAGCTT+GGG - chr1.3:54450155-54450174 MS.gene85554:CDS 15.0%
!! TTTAAAAAAAATCAATAGCT+TGG - chr1.3:54450154-54450173 MS.gene85554:CDS 15.0%
!!! TCCTATAAACATATAATTTT+GGG + chr1.3:54450681-54450700 None:intergenic 15.0%
!!! TTATTGTTTTGATGTTTTAT+TGG - chr1.3:54452055-54452074 MS.gene85554:CDS 15.0%
!! AAAAAAATATTGTGGAATCA+TGG - chr1.3:54450813-54450832 MS.gene85554:CDS 20.0%
!! AAAAAATATTGTGGAATCAT+GGG - chr1.3:54450814-54450833 MS.gene85554:CDS 20.0%
!! AAACTAATTGCTTAACAAAT+AGG + chr1.3:54451410-54451429 None:intergenic 20.0%
!! AATTATATGTTTATAGGAGT+AGG - chr1.3:54450683-54450702 MS.gene85554:intron 20.0%
!! ACATCACATAATAATTCTTA+TGG + chr1.3:54451692-54451711 None:intergenic 20.0%
!! ACCCAAAATTATATGTTTAT+AGG - chr1.3:54450677-54450696 MS.gene85554:intron 20.0%
!! CTTCCAATATTTCATTAAAA+GGG + chr1.3:54450334-54450353 None:intergenic 20.0%
!! TAAAAGACAATATTATCACA+TGG + chr1.3:54452121-54452140 None:intergenic 20.0%
!! TGCAAACTAAAAAAATATTG+TGG - chr1.3:54450805-54450824 MS.gene85554:CDS 20.0%
!! TTACTTAGATTTGAATTCAA+TGG + chr1.3:54450896-54450915 None:intergenic 20.0%
!!! ATTTTTTTAGTTTGCATTGT+TGG + chr1.3:54450801-54450820 None:intergenic 20.0%
!!! CTCCTATAAACATATAATTT+TGG + chr1.3:54450682-54450701 None:intergenic 20.0%
!!! TCTGTTTTATAACTAATTTC+CGG - chr1.3:54450943-54450962 MS.gene85554:intron 20.0%
! AAAGTTAGTACACTAAAATC+CGG + chr1.3:54450965-54450984 None:intergenic 25.0%
! AATACGTAAGACTACAAAAT+TGG + chr1.3:54450126-54450145 None:intergenic 25.0%
! ACAAAATCGAAATGATTCAA+AGG + chr1.3:54450311-54450330 None:intergenic 25.0%
! ATAACTACCATAAAATTGAG+AGG + chr1.3:54450628-54450647 None:intergenic 25.0%
! ATTATTCTTGCTACAATATG+TGG - chr1.3:54451232-54451251 MS.gene85554:intron 25.0%
! GCTTCCAATATTTCATTAAA+AGG + chr1.3:54450335-54450354 None:intergenic 25.0%
! TAAGTATAAGACTACAAAGA+TGG + chr1.3:54450103-54450122 None:intergenic 25.0%
! TTAACAAATAGGCTAGTAAA+CGG + chr1.3:54451399-54451418 None:intergenic 25.0%
! TTATTCTTGCTACAATATGT+GGG - chr1.3:54451233-54451252 MS.gene85554:intron 25.0%
!! TATATTTTCAGCAATGTCAA+GGG - chr1.3:54451209-54451228 MS.gene85554:intron 25.0%
!! TGTCCCTTTTAATGAAATAT+TGG - chr1.3:54450328-54450347 MS.gene85554:intron 25.0%
!! TTATATTTTCAGCAATGTCA+AGG - chr1.3:54451208-54451227 MS.gene85554:intron 25.0%
!!! TCACATATTTTGAAACGTTA+CGG + chr1.3:54451552-54451571 None:intergenic 25.0%
!!! TGTTGTTTTGCATAATATCA+AGG - chr1.3:54451874-54451893 MS.gene85554:intron 25.0%
!!! TTTTGTTTACTTTTTGACAG+TGG - chr1.3:54450077-54450096 MS.gene85554:CDS 25.0%
ACTGATACATATCCTGAAAT+AGG - chr1.3:54451467-54451486 MS.gene85554:intron 30.0%
AGAATACAATTGGTATTACC+AGG - chr1.3:54451264-54451283 MS.gene85554:CDS 30.0%
ATATGTTTATAGGAGTAGGA+GGG - chr1.3:54450687-54450706 MS.gene85554:intron 30.0%
ATGCAATTTGAACCTATTTC+AGG + chr1.3:54451482-54451501 None:intergenic 30.0%
ATGGTAATCTACTTCACAAT+TGG - chr1.3:54451798-54451817 MS.gene85554:CDS 30.0%
ATTAGGTAGAATGTAGAAGA+GGG - chr1.3:54450217-54450236 MS.gene85554:intron 30.0%
CTTGATTAAGAAATGCACAT+AGG + chr1.3:54450005-54450024 None:intergenic 30.0%
TATATGTTTATAGGAGTAGG+AGG - chr1.3:54450686-54450705 MS.gene85554:intron 30.0%
TCTACATTCTACCTAATCTT+TGG + chr1.3:54450214-54450233 None:intergenic 30.0%
TCTTAATCAAGTCATGGAAA+AGG - chr1.3:54450014-54450033 MS.gene85554:CDS 30.0%
TGCATTTCTTAATCAAGTCA+TGG - chr1.3:54450008-54450027 MS.gene85554:CDS 30.0%
TTGAAGGAAAAGGATGTAAA+TGG - chr1.3:54450542-54450561 MS.gene85554:CDS 30.0%
! GATTTTTAATAAGTGTGCCA+AGG + chr1.3:54451622-54451641 None:intergenic 30.0%
!!! GCGTTTCAAATGCTTTTTTT+GGG - chr1.3:54450836-54450855 MS.gene85554:CDS 30.0%
AAAAGGATGTAAATGGTCGT+AGG - chr1.3:54450549-54450568 MS.gene85554:CDS 35.0%
AGAACTGTGTCTTGTCTATA+TGG + chr1.3:54450265-54450284 None:intergenic 35.0%
ATAAGACATGATTGATGCAC+AGG - chr1.3:54451582-54451601 MS.gene85554:CDS 35.0%
ATGAAATATTGGAAGCAGAC+AGG - chr1.3:54450339-54450358 MS.gene85554:intron 35.0%
ATGTAGAAACCTATGACTTG+AGG - chr1.3:54451153-54451172 MS.gene85554:intron 35.0%
CAAATTGCATGTTCAAGTGA+TGG - chr1.3:54451492-54451511 MS.gene85554:CDS 35.0%
CCAATAACATCTGGATGTAA+TGG + chr1.3:54450500-54450519 None:intergenic 35.0%
CCATTACATCCAGATGTTAT+TGG - chr1.3:54450497-54450516 MS.gene85554:CDS 35.0%
GAGAGATGAATTGAAGGAAA+AGG - chr1.3:54450532-54450551 MS.gene85554:CDS 35.0%
GATTAGGTAGAATGTAGAAG+AGG - chr1.3:54450216-54450235 MS.gene85554:intron 35.0%
GGATAATCAGAGGAATAGTT+TGG + chr1.3:54451722-54451741 None:intergenic 35.0%
GGATCGAATCAGAATACAAT+TGG - chr1.3:54451254-54451273 MS.gene85554:CDS 35.0%
TATCACATGGACATATCACA+TGG + chr1.3:54452108-54452127 None:intergenic 35.0%
TGAAATATTGGAAGCAGACA+GGG - chr1.3:54450340-54450359 MS.gene85554:intron 35.0%
TGTGACTAACAACAATGACA+AGG - chr1.3:54452212-54452231 MS.gene85554:intron 35.0%
TTAAGAAATGCACATAGGAG+AGG + chr1.3:54450000-54450019 None:intergenic 35.0%
TTAATAAGTGTGCCAAGGTA+TGG + chr1.3:54451617-54451636 None:intergenic 35.0%
TTGAAACGTTACGGTCAAAA+AGG + chr1.3:54451543-54451562 None:intergenic 35.0%
TTGTTGGATCAAAACAACGA+CGG + chr1.3:54450785-54450804 None:intergenic 35.0%
! GCAAATGCTATTTAAGGTAG+AGG - chr1.3:54451769-54451788 MS.gene85554:CDS 35.0%
! GGATGTAATGGAGAATGATA+AGG + chr1.3:54450488-54450507 None:intergenic 35.0%
! TACGCTTCCTCTCAATTTTA+TGG - chr1.3:54450618-54450637 MS.gene85554:CDS 35.0%
! TGGCAAGCAAATGCTATTTA+AGG - chr1.3:54451763-54451782 MS.gene85554:CDS 35.0%
! TTCTTTGGCTCAATCTTAAC+AGG + chr1.3:54452366-54452385 None:intergenic 35.0%
!! TTGTCTTTTAATTCAGACCC+TGG - chr1.3:54452131-54452150 MS.gene85554:intron 35.0%
!!! GGCGTTTCAAATGCTTTTTT+TGG - chr1.3:54450835-54450854 MS.gene85554:CDS 35.0%
ACAAAAGAGTCGAGATCTGA+CGG + chr1.3:54451743-54451762 None:intergenic 40.0%
ACGACTTGTCCAATAACATC+TGG + chr1.3:54450509-54450528 None:intergenic 40.0%
AGCCTCTATCAAGTCTTCAA+TGG + chr1.3:54451120-54451139 None:intergenic 40.0%
AGCGTCTGCTAAAAAACATC+TGG - chr1.3:54450186-54450205 MS.gene85554:CDS 40.0%
AGCTTCAACTGTTGTCTCTT+TGG - chr1.3:54451064-54451083 MS.gene85554:CDS 40.0%
AGGTATAATGCTCCATACCT+TGG - chr1.3:54451602-54451621 MS.gene85554:CDS 40.0%
CAACAGTTGAAGCTGTATTC+TGG + chr1.3:54451058-54451077 None:intergenic 40.0%
CATACAGTACCTCAAGTCAT+AGG + chr1.3:54451165-54451184 None:intergenic 40.0%
GAAAAGATTGCACACCTCAT+CGG + chr1.3:54452026-54452045 None:intergenic 40.0%
GACAGGGTGGAAAAGATTAT+CGG - chr1.3:54450356-54450375 MS.gene85554:CDS 40.0%
GACCATTGAAGACTTGATAG+AGG - chr1.3:54451115-54451134 MS.gene85554:CDS 40.0%
GATTTGAATTCAATGGCCTC+TGG + chr1.3:54450889-54450908 None:intergenic 40.0%
GCTAGTAAACGGATGTTTAG+TGG + chr1.3:54451388-54451407 None:intergenic 40.0%
TGTAGAAGAGGGATCTACAT+AGG - chr1.3:54450228-54450247 MS.gene85554:intron 40.0%
TGTTGGATCAAAACAACGAC+GGG + chr1.3:54450784-54450803 None:intergenic 40.0%
TTAACAGGTTTGAGCTTGAC+AGG + chr1.3:54452351-54452370 None:intergenic 40.0%
TTTAGTGGTATCACCAACCT+TGG + chr1.3:54451373-54451392 None:intergenic 40.0%
! AACATCTGGTGCCAAAGATT+AGG - chr1.3:54450200-54450219 MS.gene85554:CDS 40.0%
! GTGGAAAGTGTAACACTAGA+AGG - chr1.3:54451338-54451357 MS.gene85554:CDS 40.0%
! TCAGATCTCGACTCTTTTGT+TGG - chr1.3:54451743-54451762 MS.gene85554:CDS 40.0%
! TTTAAGGTAGAGGTCAGTGA+TGG - chr1.3:54451779-54451798 MS.gene85554:CDS 40.0%
! TTTTATCTGACCTCTGCACT+TGG - chr1.3:54450982-54451001 MS.gene85554:intron 40.0%
!! TTTCCAGTAAGTTTGCTGCA+AGG + chr1.3:54452258-54452277 None:intergenic 40.0%
!!! TTTTGGGACTAAACTGTTGC+TGG - chr1.3:54450852-54450871 MS.gene85554:CDS 40.0%
AAAACAACGACGGGTGATGA+TGG + chr1.3:54450775-54450794 None:intergenic 45.0%
AACCAGCACAAACATTCTCC+AGG + chr1.3:54452152-54452171 None:intergenic 45.0%
AATATTGGAAGCAGACAGGG+TGG - chr1.3:54450343-54450362 MS.gene85554:intron 45.0%
ACCAGCACAAACATTCTCCA+GGG + chr1.3:54452151-54452170 None:intergenic 45.0%
AGACTTGATAGAGGCTTCTG+AGG - chr1.3:54451124-54451143 MS.gene85554:CDS 45.0%
CAAGAGAACAACTGCTGATG+AGG - chr1.3:54451835-54451854 MS.gene85554:CDS 45.0%
CAATACCGTGGTTAACCTTG+CGG - chr1.3:54452176-54452195 MS.gene85554:intron 45.0%
CAATGACAAGGAGCAAGAAG+TGG - chr1.3:54452224-54452243 MS.gene85554:intron 45.0%
CGTCGAGAGAGATGAATTGA+AGG - chr1.3:54450526-54450545 MS.gene85554:CDS 45.0%
GAGGGTTCATTGAACACTGT+GGG - chr1.3:54450705-54450724 MS.gene85554:intron 45.0%
GCAGCAAACTTACTGGAAAG+AGG - chr1.3:54452259-54452278 MS.gene85554:intron 45.0%
GCTAATTGATCTCACACTGC+AGG - chr1.3:54450577-54450596 MS.gene85554:CDS 45.0%
GTTGACATTGAATGCCGATG+AGG - chr1.3:54452009-54452028 MS.gene85554:CDS 45.0%
! GAGATCTGACGGATAATCAG+AGG + chr1.3:54451732-54451751 None:intergenic 45.0%
!! ATGATGGATCATGAGCAGTC+AGG + chr1.3:54450759-54450778 None:intergenic 45.0%
!! GTTCAAGTGATGGACAACAC+AGG - chr1.3:54451502-54451521 MS.gene85554:CDS 45.0%
AAGGAATGCAGTGCCAAGGT+TGG - chr1.3:54451357-54451376 MS.gene85554:intron 50.0%
ACATCTCCAGCATCAGCAGA+CGG + chr1.3:54452315-54452334 None:intergenic 50.0%
ACCCTGGAGAATGTTTGTGC+TGG - chr1.3:54452147-54452166 MS.gene85554:intron 50.0%
ACGCCTTGCAGCAAACTTAC+TGG - chr1.3:54452252-54452271 MS.gene85554:intron 50.0%
AGCATCCGCAAGGTTAACCA+CGG + chr1.3:54452184-54452203 None:intergenic 50.0%
AGGCATGTACAAAGTGCGCA+GGG - chr1.3:54451284-54451303 MS.gene85554:CDS 50.0%
CACATCTCCACCATCAAGTC+TGG + chr1.3:54451015-54451034 None:intergenic 50.0%
CGCAAGGTTAACCACGGTAT+TGG + chr1.3:54452178-54452197 None:intergenic 50.0%
CTAGAAGGAATGCAGTGCCA+AGG - chr1.3:54451353-54451372 MS.gene85554:intron 50.0%
GGAGGGTTCATTGAACACTG+TGG - chr1.3:54450704-54450723 MS.gene85554:intron 50.0%
GGGACTAAACTGTTGCTGGA+TGG - chr1.3:54450856-54450875 MS.gene85554:CDS 50.0%
GTTGCAGGTCGTCAGTATTG+TGG - chr1.3:54451319-54451338 MS.gene85554:CDS 50.0%
TGGTTGCTGACCAGACTTGA+TGG - chr1.3:54451002-54451021 MS.gene85554:intron 50.0%
TTGCTGACCAGACTTGATGG+TGG - chr1.3:54451005-54451024 MS.gene85554:intron 50.0%
! TGTGGGCTAACTATGCTGAG+AGG - chr1.3:54450722-54450741 MS.gene85554:intron 50.0%
!! TGCTGGTTCATCCAATACCG+TGG - chr1.3:54452164-54452183 MS.gene85554:intron 50.0%
ATCTCCAGCATCAGCAGACG+GGG + chr1.3:54452313-54452332 None:intergenic 55.0%
CAGGCATGTACAAAGTGCGC+AGG - chr1.3:54451283-54451302 MS.gene85554:CDS 55.0%
CATCTCCAGCATCAGCAGAC+GGG + chr1.3:54452314-54452333 None:intergenic 55.0%
CTGCGCACTTTGTACATGCC+TGG + chr1.3:54451285-54451304 None:intergenic 55.0%
GGGAGAGTGAGAACAGTTGC+AGG - chr1.3:54451304-54451323 MS.gene85554:CDS 55.0%
TGGTCAGCAACCAAGTGCAG+AGG + chr1.3:54450995-54451014 None:intergenic 55.0%
!! CATGATCGAGAGCATCCGCA+AGG + chr1.3:54452194-54452213 None:intergenic 55.0%
TTGACCCCGTCTGCTGATGC+TGG - chr1.3:54452306-54452325 MS.gene85554:intron 60.0%
! GCTGGATGGTGACTTGCCAG+AGG - chr1.3:54450870-54450889 MS.gene85554:CDS 60.0%


Chromosome Type Strat End Strand Name
chr1.3 gene 54449991 54452389 54449991 ID=MS.gene85554
chr1.3 mRNA 54449991 54452389 54449991 ID=MS.gene85554.t1;Parent=MS.gene85554
chr1.3 exon 54452345 54452389 54452345 ID=MS.gene85554.t1.exon1;Parent=MS.gene85554.t1
chr1.3 CDS 54452345 54452389 54452345 ID=cds.MS.gene85554.t1;Parent=MS.gene85554.t1
chr1.3 exon 54452003 54452147 54452003 ID=MS.gene85554.t1.exon2;Parent=MS.gene85554.t1
chr1.3 CDS 54452003 54452147 54452003 ID=cds.MS.gene85554.t1;Parent=MS.gene85554.t1
chr1.3 exon 54451741 54451870 54451741 ID=MS.gene85554.t1.exon3;Parent=MS.gene85554.t1
chr1.3 CDS 54451741 54451870 54451741 ID=cds.MS.gene85554.t1;Parent=MS.gene85554.t1
chr1.3 exon 54451469 54451636 54451469 ID=MS.gene85554.t1.exon4;Parent=MS.gene85554.t1
chr1.3 CDS 54451469 54451636 54451469 ID=cds.MS.gene85554.t1;Parent=MS.gene85554.t1
chr1.3 exon 54451235 54451364 54451235 ID=MS.gene85554.t1.exon5;Parent=MS.gene85554.t1
chr1.3 CDS 54451235 54451364 54451235 ID=cds.MS.gene85554.t1;Parent=MS.gene85554.t1
chr1.3 exon 54451006 54451160 54451006 ID=MS.gene85554.t1.exon6;Parent=MS.gene85554.t1
chr1.3 CDS 54451006 54451160 54451006 ID=cds.MS.gene85554.t1;Parent=MS.gene85554.t1
chr1.3 exon 54450801 54450891 54450801 ID=MS.gene85554.t1.exon7;Parent=MS.gene85554.t1
chr1.3 CDS 54450801 54450891 54450801 ID=cds.MS.gene85554.t1;Parent=MS.gene85554.t1
chr1.3 exon 54450485 54450661 54450485 ID=MS.gene85554.t1.exon8;Parent=MS.gene85554.t1
chr1.3 CDS 54450485 54450661 54450485 ID=cds.MS.gene85554.t1;Parent=MS.gene85554.t1
chr1.3 exon 54450350 54450406 54450350 ID=MS.gene85554.t1.exon9;Parent=MS.gene85554.t1
chr1.3 CDS 54450350 54450406 54450350 ID=cds.MS.gene85554.t1;Parent=MS.gene85554.t1
chr1.3 exon 54449991 54450233 54449991 ID=MS.gene85554.t1.exon10;Parent=MS.gene85554.t1
chr1.3 CDS 54449991 54450233 54449991 ID=cds.MS.gene85554.t1;Parent=MS.gene85554.t1
Gene Sequence

>MS.gene85554

ATGACACCTCTCCTATGTGCATTTCTTAATCAAGTCATGGAAAAGGTCATAACTCTCATTATGAACACATTTCTTCAACTAATACATTTTGTTTACTTTTTGACAGTGGCCATCTTTGTAGTCTTATACTTACCAATTTTGTAGTCTTACGTATTACTTTTTTTTTAAAAAAAATCAATAGCTTGGGAAGATACAAGCGTCTGCTAAAAAACATCTGGTGCCAAAGATTAGGTAGAATGTAGAAGAGGGATCTACATAGGTTCAAGCTAAACCATATAGACAAGACACAGTTCTTTAAGATTGATGCAAGCAACATTCCTTTGAATCATTTCGATTTTGTCCCTTTTAATGAAATATTGGAAGCAGACAGGGTGGAAAAGATTATCGGTAAGTGCATAATGCTGCAATGTTATAATTTAGAAACATATTTATTATAGATGACATCATTAAGTTTTATTAATTTGATCAAACTTATTGTATTTTGCTGATAAGCTCCTTATCATTCTCCATTACATCCAGATGTTATTGGACAAGTCGTCGAGAGAGATGAATTGAAGGAAAAGGATGTAAATGGTCGTAGGAGTAAGCTAATTGATCTCACACTGCAGGATTCTGAGTTAGTTGCTTTACGCTTCCTCTCAATTTTATGGTAGTTATATTCGTTAAGTAAATTTTGTTAAGTAGTAACCCAAAATTATATGTTTATAGGAGTAGGAGGGTTCATTGAACACTGTGGGCTAACTATGCTGAGAGGATGAATTCATTCCTGACTGCTCATGATCCATCATCACCCGTCGTTGTTTTGATCCAACAATGCAAACTAAAAAAATATTGTGGAATCATGGGCGTTTCAAATGCTTTTTTTGGGACTAAACTGTTGCTGGATGGTGACTTGCCAGAGGCCATTGAATTCAAATCTAAGTAATAAAAACGTGTCTAATTTTGTGATTATTCTGTTTTATAACTAATTTCCGGATTTTAGTGTACTAACTTTTATCTGACCTCTGCACTTGGTTGCTGACCAGACTTGATGGTGGAGATGTGCAAGTATCACAGTGTGTAAGCCAGAATACAGCTTCAACTGTTGTCTCTTTGGTTGATGATATGCTTCAGACTAAGAGAATGACCATTGAAGACTTGATAGAGGCTTCTGAGGTATGCTATGTAGAAACCTATGACTTGAGGTACTGTATGTCTAATAGTCTAACATAACTGTTTTATATTTTCAGCAATGTCAAGGGATTATTCTTGCTACAATATGTGGGATCGAATCAGAATACAATTGGTATTACCAGGCATGTACAAAGTGCGCAGGGAGAGTGAGAACAGTTGCAGGTCGTCAGTATTGTGGAAAGTGTAACACTAGAAGGAATGCAGTGCCAAGGTTGGTGATACCACTAAACATCCGTTTACTAGCCTATTTGTTAAGCAATTAGTTTTAATTGTGCATGTTGTTGTTTGTTAGCTTATTTATCAACTGATACATATCCTGAAATAGGTTCAAATTGCATGTTCAAGTGATGGACAACACAGGATCAACAAGCTTTATCCTTTTTGACCGTAACGTTTCAAAATATGTGAACAAAACTGTATAAGACATGATTGATGCACAGGTATAATGCTCCATACCTTGGCACACTTATTAAAAATCAGAATTGACGTGTTTGTGTAGTTTTCGACTTTGTGTGCATTTTCTAACCATAAGAATTATTATGTGATGTCAAACAGCCAAACTATTCCTCTGATTATCCGTCAGATCTCGACTCTTTTGTTGGCAAGCAAATGCTATTTAAGGTAGAGGTCAGTGATGGTAATCTACTTCACAATTGGCACAATTACGCTGTCAAGAGAACAACTGCTGATGAGGATATTATCAATCGCTTTGTTGTTTTGCATAATATCAAGGTATATTAATCACTTGCATCATTGACTACTTTTTGCTTTTGAATTTCTAAATCTTATAGATTTACTTTGCACATACAGCTTTCTGATAATGAAGATGTAGCATATGAGACGTTGTTGACATTGAATGCCGATGAGGTGTGCAATCTTTTCTTTTTATAATTATTGTTTTGATGTTTTATTGGATACATGTTATATTTAAATATAAATGTCCATGTGATATGTCCATGTGATAATATTGTCTTTTAATTCAGACCCTGGAGAATGTTTGTGCTGGTTCATCCAATACCGTGGTTAACCTTGCGGATGCTCTCGATCATGTGACTAACAACAATGACAAGGAGCAAGAAGTGGATACTACGCCTTGCAGCAAACTTACTGGAAAGAGGTCTGCTGAAAAAGACTTGATGATTTTGACCCCGTCTGCTGATGCTGGAGATGTCTCTGCTACAATGCCTGTCAAGCTCAAACCTGTTAAGATTGAGCCAAAGAACTAA
Protein sequence

>MS.gene85554.t1

MTPLLCAFLNQVMEKKRDLHRFKLNHIDKTQFFKIDASNIPLNHFDFVPFNEILEADRVEKIIDVIGQVVERDELKEKDVNGRRSKLIDLTLQDSELVALRFLSILWMNSFLTAHDPSSPVVVLIQQCKLKKYCGIMGVSNAFFGTKLLLDGDLPEAIEFKSKLDGGDVQVSQCVSQNTASTVVSLVDDMLQTKRMTIEDLIEASEQCQGIILATICGIESEYNWYYQACTKCAGRVRTVAGRQYCGKCNTRRNAVPRFKLHVQVMDNTGSTSFILFDRNVSKYVNKTPNYSSDYPSDLDSFVGKQMLFKVEVSDGNLLHNWHNYAVKRTTADEDIINRFVVLHNIKLSDNEDVAYETLLTLNADETLENVCAGSSNTVVNLADALDHVTNNNDKEQEVDTTPCSKLTGKRSAEKDLMILTPSADAGDVSATMPVKLKPVKIEPKN