AlfalfaGEDB | MS.gene85636

M. sativa cultivar XinJiangDaYe / MS.gene85636

KEGG: -
GO: -
PFAM: PF05553.14 PF14364.9

Nr:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene85636.t1	ABN08745.1	76.6	222	51	1	1	221	1	222	2.90E-81	311.6

Swissprot:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score

Trembl:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene85636.t1	A2Q545	76.6	222	51	1	1	221	1	222	2.1e-81	311.6

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network:

Gene1	Gene2	correlation coefficient	p_value	FDR
MS.gene055150	MS.gene85636	0.824088	9.76E-54	-1.69E-46
MS.gene05624	MS.gene85636	0.812745	3.63E-51	-1.69E-46
MS.gene05878	MS.gene85636	0.812784	3.56E-51	-1.69E-46
MS.gene05952	MS.gene85636	0.841825	3.76E-58	-1.69E-46

PPI:

Gene1	Gene2	Type

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene85636.t1	MTR_2g039320	83.333	222	36	1	1	221	1	222	3.03e-122	346
MS.gene85636.t1	MTR_4g126210	37.349	249	100	7	12	216	7	243	6.24e-33	119

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score

Find 58 sgRNAs with CRISPR-Local

Find 66 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
CGTTCATCGTCGTCGTTTCT+TGG	0.238445	2.1:+48035276	MS.gene85636:CDS
TTGAGTTCCAGTTCTGATTC+AGG	0.253071	2.1:-48035609	None:intergenic
CTCTTGCAACGGCTGATGTC+GGG	0.305557	2.1:+48035537	MS.gene85636:CDS
TTAATCGTTAACCTTGTCAT+AGG	0.350745	2.1:+48035183	MS.gene85636:CDS
GACGATGAACGGACAAGTTC+CGG	0.363566	2.1:-48035264	None:intergenic
GGTTCTTAACCTGTTCGACC+TGG	0.383481	2.1:-48035380	None:intergenic
GTTGTTGCTGCTGCTGATGC+TGG	0.384572	2.1:-48035339	None:intergenic
TGTTCGACCTGGGTTTCTTC+CGG	0.407677	2.1:-48035369	None:intergenic
AAGACTTGACCCGGGTCAAC+AGG	0.410251	2.1:+48035403	MS.gene85636:CDS
GACAACATCGTCTACTTTGT+TGG	0.414991	2.1:+48035425	MS.gene85636:CDS
AAAGGTTTAAGCAGCAACTT+AGG	0.427984	2.1:+48035703	MS.gene85636:CDS
GAAGAAACCTGAATCAGAAC+TGG	0.438657	2.1:+48035602	MS.gene85636:CDS
TTTACAGCAATTGAGTCTAA+AGG	0.445981	2.1:-48035310	None:intergenic
TGTTGACCCGGGTCAAGTCT+TGG	0.450238	2.1:-48035401	None:intergenic
GTTTAAGCAGCAACTTAGGT+TGG	0.453539	2.1:+48035707	MS.gene85636:CDS
TTCGGTAAAGGTGTTGGAGG+AGG	0.453986	2.1:+48035578	MS.gene85636:CDS
CCTCTTGCAACGGCTGATGT+CGG	0.457220	2.1:+48035536	MS.gene85636:CDS
TATATGATATAATTGTAAGT+TGG	0.457863	2.1:+48035139	MS.gene85636:CDS
CATCGTCTACTTTGTTGGAC+CGG	0.457887	2.1:+48035430	MS.gene85636:CDS
GGAAGAAACCCAGGTCGAAC+AGG	0.460194	2.1:+48035371	MS.gene85636:CDS
ACGATGTTGTCCTGTTGACC+CGG	0.483259	2.1:-48035413	None:intergenic
ATTAGATTCGGTAAAGGTGT+TGG	0.486940	2.1:+48035572	MS.gene85636:CDS
TGTGAGAGGAAGGGAAGAAG+AGG	0.501710	2.1:+48035647	MS.gene85636:CDS
AAAGAAAGTTTGTGAGAGGA+AGG	0.508582	2.1:+48035637	MS.gene85636:CDS
AGATTCGGTAAAGGTGTTGG+AGG	0.508799	2.1:+48035575	MS.gene85636:CDS
GTTCTTAACCTGTTCGACCT+GGG	0.514128	2.1:-48035379	None:intergenic
CCGGGTCAAGTCAATCGACC+TGG	0.519795	2.1:+48035449	MS.gene85636:CDS
AGCAGCAACAACAACAACGC+CGG	0.524549	2.1:+48035350	MS.gene85636:CDS
AGCAGCAACTTAGGTTGGAA+AGG	0.527408	2.1:+48035712	MS.gene85636:CDS
TCATGGATTAGATTCGGTAA+AGG	0.535518	2.1:+48035566	MS.gene85636:CDS
ACAGAAATGAATGGAGGCCC+AGG	0.538526	2.1:+48035483	MS.gene85636:CDS
GTTGCAAGAGGGACGGAGCT+CGG	0.541927	2.1:-48035524	None:intergenic
AGAGGAAGAAGTTGATGCAA+AGG	0.542473	2.1:+48035665	MS.gene85636:CDS
ATCGTCTACTTTGTTGGACC+GGG	0.548993	2.1:+48035431	MS.gene85636:CDS
ACGGCAACTCGACTCGCCGC+CGG	0.549447	2.1:+48035245	MS.gene85636:CDS
TCTTGCAACGGCTGATGTCG+GGG	0.551303	2.1:+48035538	MS.gene85636:CDS
CCAGGTCGATTGACTTGACC+CGG	0.552190	2.1:-48035449	None:intergenic
AGGGACGGAGCTCGGATCAA+CGG	0.556439	2.1:-48035516	None:intergenic
AAGAAAGTTTGTGAGAGGAA+GGG	0.559930	2.1:+48035638	MS.gene85636:CDS
CAAGAAACGACGACGATGAA+CGG	0.560499	2.1:-48035275	None:intergenic
ATCAACGGCGTAGAATCACC+TGG	0.576704	2.1:-48035501	None:intergenic
AAAATGAGAAGTAATAGCTA+TGG	0.577116	2.1:-48035208	None:intergenic
TTAAGAACCAAGACTTGACC+CGG	0.581525	2.1:+48035394	MS.gene85636:CDS
AACAACGCCGGAAGAAACCC+AGG	0.587857	2.1:+48035362	MS.gene85636:CDS
CTCTGTAAAACAGAAATGAA+TGG	0.588052	2.1:+48035474	MS.gene85636:CDS
CCGACATCAGCCGTTGCAAG+AGG	0.595808	2.1:-48035536	None:intergenic
CGGGTCAAGTCAATCGACCT+GGG	0.600972	2.1:+48035450	MS.gene85636:CDS
CGACATCAGCCGTTGCAAGA+GGG	0.606146	2.1:-48035535	None:intergenic
CGATGTTGTCCTGTTGACCC+GGG	0.610417	2.1:-48035412	None:intergenic
ATCAGCCGTTGCAAGAGGGA+CGG	0.611532	2.1:-48035531	None:intergenic
CCTAAAAGAAAGTTTGTGAG+AGG	0.620662	2.1:+48035633	MS.gene85636:CDS
GAGCTCCGTCCCTCTTGCAA+CGG	0.641505	2.1:+48035526	MS.gene85636:CDS
TGTAAAACAGAAATGAATGG+AGG	0.671074	2.1:+48035477	MS.gene85636:CDS
TAGCTATGGTACCTATGACA+AGG	0.682585	2.1:-48035194	None:intergenic
TCAACGGCGTAGAATCACCT+GGG	0.691795	2.1:-48035500	None:intergenic
GATGAACGGACAAGTTCCGG+CGG	0.719507	2.1:-48035261	None:intergenic
ACGATTAAGAATATACACGA+AGG	0.720504	2.1:-48035168	None:intergenic
TAAGAACCAAGACTTGACCC+GGG	0.732074	2.1:+48035395	MS.gene85636:CDS

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!!	TATATGATATAATTGTAAGT+TGG	+	chr2.1:48035139-48035158	MS.gene85636:CDS	15.0%
!!!	ATTTTGCCACTAAAAAAAAA+CGG	+	chr2.1:48035226-48035245	MS.gene85636:CDS	20.0%
!	AAAATGAGAAGTAATAGCTA+TGG	-	chr2.1:48035211-48035230	None:intergenic	25.0%
!!	AGGCTGATGATTTTATAAAA+AGG	+	chr2.1:48035685-48035704	MS.gene85636:CDS	25.0%
!!!	GAATTTTCATGGATTAGATT+CGG	+	chr2.1:48035560-48035579	MS.gene85636:CDS	25.0%
	ACGATTAAGAATATACACGA+AGG	-	chr2.1:48035171-48035190	None:intergenic	30.0%
	CTCTGTAAAACAGAAATGAA+TGG	+	chr2.1:48035474-48035493	MS.gene85636:CDS	30.0%
	TGTAAAACAGAAATGAATGG+AGG	+	chr2.1:48035477-48035496	MS.gene85636:CDS	30.0%
	TTAATCGTTAACCTTGTCAT+AGG	+	chr2.1:48035183-48035202	MS.gene85636:CDS	30.0%
	TTTACAGCAATTGAGTCTAA+AGG	-	chr2.1:48035313-48035332	None:intergenic	30.0%
!	CATTCATTTCTGTTTTACAG+AGG	-	chr2.1:48035476-48035495	None:intergenic	30.0%
	AAAGAAAGTTTGTGAGAGGA+AGG	+	chr2.1:48035637-48035656	MS.gene85636:CDS	35.0%
	AAAGGTTTAAGCAGCAACTT+AGG	+	chr2.1:48035703-48035722	MS.gene85636:CDS	35.0%
	AAGAAAGTTTGTGAGAGGAA+GGG	+	chr2.1:48035638-48035657	MS.gene85636:CDS	35.0%
	CCTAAAAGAAAGTTTGTGAG+AGG	+	chr2.1:48035633-48035652	MS.gene85636:CDS	35.0%
!	ATTAGATTCGGTAAAGGTGT+TGG	+	chr2.1:48035572-48035591	MS.gene85636:CDS	35.0%
!	CCTCTCACAAACTTTCTTTT+AGG	-	chr2.1:48035636-48035655	None:intergenic	35.0%
!!	TCATGGATTAGATTCGGTAA+AGG	+	chr2.1:48035566-48035585	MS.gene85636:CDS	35.0%
!!!	GAGTTGCCGTTTTTTTTTAG+TGG	-	chr2.1:48035235-48035254	None:intergenic	35.0%
	GAAGAAACCTGAATCAGAAC+TGG	+	chr2.1:48035602-48035621	MS.gene85636:CDS	40.0%
	GACAACATCGTCTACTTTGT+TGG	+	chr2.1:48035425-48035444	MS.gene85636:CDS	40.0%
	GTTTAAGCAGCAACTTAGGT+TGG	+	chr2.1:48035707-48035726	MS.gene85636:CDS	40.0%
	TAGCTATGGTACCTATGACA+AGG	-	chr2.1:48035197-48035216	None:intergenic	40.0%
	TTAAGAACCAAGACTTGACC+CGG	+	chr2.1:48035394-48035413	MS.gene85636:CDS	40.0%
!	TTGAGTTCCAGTTCTGATTC+AGG	-	chr2.1:48035612-48035631	None:intergenic	40.0%
!!	AGAGGAAGAAGTTGATGCAA+AGG	+	chr2.1:48035665-48035684	MS.gene85636:CDS	40.0%
	ATCGTCTACTTTGTTGGACC+GGG	+	chr2.1:48035431-48035450	MS.gene85636:CDS	45.0%
	CAAGAAACGACGACGATGAA+CGG	-	chr2.1:48035278-48035297	None:intergenic	45.0%
	CATCGTCTACTTTGTTGGAC+CGG	+	chr2.1:48035430-48035449	MS.gene85636:CDS	45.0%
	GTTCTTAACCTGTTCGACCT+GGG	-	chr2.1:48035382-48035401	None:intergenic	45.0%
	TAAGAACCAAGACTTGACCC+GGG	+	chr2.1:48035395-48035414	MS.gene85636:CDS	45.0%
!	CTGATGTCGGGGAATTTTCA+TGG	+	chr2.1:48035549-48035568	MS.gene85636:CDS	45.0%
!	TTTCTGTTTTACAGAGGCCC+AGG	-	chr2.1:48035470-48035489	None:intergenic	45.0%
!!	AGATTCGGTAAAGGTGTTGG+AGG	+	chr2.1:48035575-48035594	MS.gene85636:CDS	45.0%
!!	AGCAGCAACTTAGGTTGGAA+AGG	+	chr2.1:48035712-48035731	MS.gene85636:CDS	45.0%
	ACAGAAATGAATGGAGGCCC+AGG	+	chr2.1:48035483-48035502	MS.gene85636:CDS	50.0%
	ACGATGTTGTCCTGTTGACC+CGG	-	chr2.1:48035416-48035435	None:intergenic	50.0%
	AGCAGCAACAACAACAACGC+CGG	+	chr2.1:48035350-48035369	MS.gene85636:CDS	50.0%
	ATCAACGGCGTAGAATCACC+TGG	-	chr2.1:48035504-48035523	None:intergenic	50.0%
	CGTTCATCGTCGTCGTTTCT+TGG	+	chr2.1:48035276-48035295	MS.gene85636:CDS	50.0%
	GACGATGAACGGACAAGTTC+CGG	-	chr2.1:48035267-48035286	None:intergenic	50.0%
	GGTTCTTAACCTGTTCGACC+TGG	-	chr2.1:48035383-48035402	None:intergenic	50.0%
	TCAACGGCGTAGAATCACCT+GGG	-	chr2.1:48035503-48035522	None:intergenic	50.0%
	TGTGAGAGGAAGGGAAGAAG+AGG	+	chr2.1:48035647-48035666	MS.gene85636:CDS	50.0%
	TGTTCGACCTGGGTTTCTTC+CGG	-	chr2.1:48035372-48035391	None:intergenic	50.0%
!!	TTCGGTAAAGGTGTTGGAGG+AGG	+	chr2.1:48035578-48035597	MS.gene85636:CDS	50.0%
	AACAACGCCGGAAGAAACCC+AGG	+	chr2.1:48035362-48035381	MS.gene85636:CDS	55.0%
	AAGACTTGACCCGGGTCAAC+AGG	+	chr2.1:48035403-48035422	MS.gene85636:CDS	55.0%
	ATCAGCCGTTGCAAGAGGGA+CGG	-	chr2.1:48035534-48035553	None:intergenic	55.0%
	CCTCTTGCAACGGCTGATGT+CGG	+	chr2.1:48035536-48035555	MS.gene85636:CDS	55.0%
	CGACATCAGCCGTTGCAAGA+GGG	-	chr2.1:48035538-48035557	None:intergenic	55.0%
	CGATGTTGTCCTGTTGACCC+GGG	-	chr2.1:48035415-48035434	None:intergenic	55.0%
	CGGGTCAAGTCAATCGACCT+GGG	+	chr2.1:48035450-48035469	MS.gene85636:CDS	55.0%
	CTCTTGCAACGGCTGATGTC+GGG	+	chr2.1:48035537-48035556	MS.gene85636:CDS	55.0%
	GATGAACGGACAAGTTCCGG+CGG	-	chr2.1:48035264-48035283	None:intergenic	55.0%
	GGAAGAAACCCAGGTCGAAC+AGG	+	chr2.1:48035371-48035390	MS.gene85636:CDS	55.0%
	GTTGTTGCTGCTGCTGATGC+TGG	-	chr2.1:48035342-48035361	None:intergenic	55.0%
	TCTTGCAACGGCTGATGTCG+GGG	+	chr2.1:48035538-48035557	MS.gene85636:CDS	55.0%
	TGTTGACCCGGGTCAAGTCT+TGG	-	chr2.1:48035404-48035423	None:intergenic	55.0%
!!	CCAGGTCGATTGACTTGACC+CGG	-	chr2.1:48035452-48035471	None:intergenic	55.0%
	AGGGACGGAGCTCGGATCAA+CGG	-	chr2.1:48035519-48035538	None:intergenic	60.0%
	CCGACATCAGCCGTTGCAAG+AGG	-	chr2.1:48035539-48035558	None:intergenic	60.0%
	CCGGGTCAAGTCAATCGACC+TGG	+	chr2.1:48035449-48035468	MS.gene85636:CDS	60.0%
	GAGCTCCGTCCCTCTTGCAA+CGG	+	chr2.1:48035526-48035545	MS.gene85636:CDS	60.0%
	GTTGCAAGAGGGACGGAGCT+CGG	-	chr2.1:48035527-48035546	None:intergenic	60.0%
!	ACGGCAACTCGACTCGCCGC+CGG	+	chr2.1:48035245-48035264	MS.gene85636:CDS	70.0%

Chromosome	Type	Strat	End	Strand	Name
chr2.1	gene	48035114	48035779	48035114	ID=MS.gene85636
chr2.1	mRNA	48035114	48035779	48035114	ID=MS.gene85636.t1;Parent=MS.gene85636
chr2.1	exon	48035114	48035779	48035114	ID=MS.gene85636.t1.exon1;Parent=MS.gene85636.t1
chr2.1	CDS	48035114	48035779	48035114	ID=cds.MS.gene85636.t1;Parent=MS.gene85636.t1

Gene Sequence

>MS.gene85636

ATGGGTGACGAAGATTCTGTATCAATATATGATATAATTGTAAGTTGGTTTACTCCTTCGTGTATATTCTTAATCGTTAACCTTGTCATAGGTACCATAGCTATTACTTCTCATTTTGCCACTAAAAAAAAACGGCAACTCGACTCGCCGCCGGAACTTGTCCGTTCATCGTCGTCGTTTCTTGGACGAGTCACATCCTTTAGACTCAATTGCTGTAAATTTGAACCAGCATCAGCAGCAGCAACAACAACAACGCCGGAAGAAACCCAGGTCGAACAGGTTAAGAACCAAGACTTGACCCGGGTCAACAGGACAACATCGTCTACTTTGTTGGACCGGGTCAAGTCAATCGACCTGGGCCTCTGTAAAACAGAAATGAATGGAGGCCCAGGTGATTCTACGCCGTTGATCCGAGCTCCGTCCCTCTTGCAACGGCTGATGTCGGGGAATTTTCATGGATTAGATTCGGTAAAGGTGTTGGAGGAGGAGAAGAAACCTGAATCAGAACTGGAACTCAAACCTAAAAGAAAGTTTGTGAGAGGAAGGGAAGAAGAGGAAGAAGTTGATGCAAAGGCTGATGATTTTATAAAAAGGTTTAAGCAGCAACTTAGGTTGGAAAGGCTTGATTCTATTTTACGTTATAAAGACATAGTTCATAGAAACTGA

Protein sequence

>MS.gene85636.t1

MGDEDSVSIYDIIVSWFTPSCIFLIVNLVIGTIAITSHFATKKKRQLDSPPELVRSSSSFLGRVTSFRLNCCKFEPASAAATTTTPEETQVEQVKNQDLTRVNRTTSSTLLDRVKSIDLGLCKTEMNGGPGDSTPLIRAPSLLQRLMSGNFHGLDSVKVLEEEKKPESELELKPKRKFVRGREEEEEVDAKADDFIKRFKQQLRLERLDSILRYKDIVHRN