AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene05878


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene05878.t1 MTR_8g102755 89.855 207 21 0 1 207 1 207 2.38e-140 390
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score

Find 31 sgRNAs with CRISPR-Local

Find 44 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TAATGATATTGAAATGAATT+TGG 0.220117 4.4:-3399713 MS.gene05878:CDS
AAGACAGAAACAAAGAAATT+TGG 0.303541 4.4:-3400099 MS.gene05878:CDS
ATTGAAGATGATGCTACTTT+TGG 0.318948 4.4:-3400154 MS.gene05878:CDS
CAAAGCTTTCATGATGAAAA+TGG 0.325691 4.4:-3399832 MS.gene05878:CDS
CAGGTTTGCAATCAAGAAAT+AGG 0.331402 4.4:-3399621 MS.gene05878:CDS
ACTGTTCATGTTGATACTTC+AGG 0.350166 4.4:-3399640 MS.gene05878:CDS
ATTCAAATCAAGCTTGTTGC+TGG 0.398775 4.4:-3400075 MS.gene05878:CDS
ATCTATTACACCATTATTTG+CGG 0.399079 4.4:+3399861 None:intergenic
GGATGTGGACATAAATTGTT+TGG 0.414508 4.4:-3400054 MS.gene05878:CDS
GAATCTACCTTTCCTGAGTC+AGG 0.424703 4.4:+3399991 None:intergenic
AAAAGGGTCTAACGTAAAAC+AGG 0.453639 4.4:-3400178 MS.gene05878:CDS
ATCAAGCTTGTTGCTGGATG+TGG 0.461106 4.4:-3400069 MS.gene05878:CDS
GTGGAGAGTGCCGCAAATAA+TGG 0.466076 4.4:-3399871 MS.gene05878:CDS
AGAATGGAGTATCAATCTCT+TGG 0.471194 4.4:-3400216 MS.gene05878:CDS
CATAAATTGTTTGGACGGAA+AGG 0.490271 4.4:-3400045 MS.gene05878:CDS
GATATTGAAATGAATTTGGA+AGG 0.502773 4.4:-3399709 MS.gene05878:CDS
TCAGGAAAAGCTCTTTCATC+GGG 0.511579 4.4:+3400009 None:intergenic
ATAAATTGTTTGGACGGAAA+GGG 0.528425 4.4:-3400044 MS.gene05878:CDS
GTCAGGAAAAGCTCTTTCAT+CGG 0.531583 4.4:+3400008 None:intergenic
AGTATTTATGAAGTCACGTA+TGG 0.536293 4.4:-3399742 MS.gene05878:CDS
TCAGGAAAGGTAGATTCTGT+TGG 0.537763 4.4:-3399985 MS.gene05878:CDS
GTAGACGAAGAAATTAATAC+AGG 0.538396 4.4:-3399667 MS.gene05878:CDS
GTGGACATAAATTGTTTGGA+CGG 0.566125 4.4:-3400050 MS.gene05878:CDS
TGGAGTATCAATCTCTTGGA+AGG 0.588423 4.4:-3400212 MS.gene05878:CDS
ACAACTTGGAGTTGAAGAGA+AGG 0.600247 4.4:-3399917 MS.gene05878:CDS
CAACTTGGAGTTGAAGAGAA+GGG 0.601023 4.4:-3399916 MS.gene05878:CDS
CTTGATTTGATATAACAACC+AGG 0.620765 4.4:+3400122 None:intergenic
GTTTGCAATCAAGAAATAGG+TGG 0.639196 4.4:-3399618 MS.gene05878:CDS
AAATAATGGTGTAATAGATG+AGG 0.639630 4.4:-3399857 MS.gene05878:CDS
GAAAATGGTGTTCCGCCTGA+CGG 0.653575 4.4:-3399817 MS.gene05878:CDS
AATGGTGTTCCGCCTGACGG+TGG 0.691121 4.4:-3399814 MS.gene05878:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TAATGATATTGAAATGAATT+TGG - chr4.4:3400112-3400131 MS.gene05878:CDS 15.0%
!!! CAACTTTTAGAATAGTAAAT+TGG - chr4.4:3400048-3400067 MS.gene05878:CDS 20.0%
! AAATAATGGTGTAATAGATG+AGG - chr4.4:3399968-3399987 MS.gene05878:CDS 25.0%
! AAGACAGAAACAAAGAAATT+TGG - chr4.4:3399726-3399745 MS.gene05878:CDS 25.0%
! ATCTATTACACCATTATTTG+CGG + chr4.4:3399967-3399986 None:intergenic 25.0%
! GATATTGAAATGAATTTGGA+AGG - chr4.4:3400116-3400135 MS.gene05878:CDS 25.0%
!! AAAGAAAAAGAAGTACAACT+TGG - chr4.4:3399894-3399913 MS.gene05878:CDS 25.0%
AAACAAGATGCAGAAAAATG+AGG - chr4.4:3399932-3399951 MS.gene05878:CDS 30.0%
AGTATTTATGAAGTCACGTA+TGG - chr4.4:3400083-3400102 MS.gene05878:CDS 30.0%
ATAAATTGTTTGGACGGAAA+GGG - chr4.4:3399781-3399800 MS.gene05878:CDS 30.0%
CAAAGCTTTCATGATGAAAA+TGG - chr4.4:3399993-3400012 MS.gene05878:CDS 30.0%
GTAGACGAAGAAATTAATAC+AGG - chr4.4:3400158-3400177 MS.gene05878:CDS 30.0%
! CATTTTCATCATGAAAGCTT+TGG + chr4.4:3399995-3400014 None:intergenic 30.0%
! CTTGATTTGATATAACAACC+AGG + chr4.4:3399706-3399725 None:intergenic 30.0%
!!! ATTGAAGATGATGCTACTTT+TGG - chr4.4:3399671-3399690 MS.gene05878:CDS 30.0%
AAAAGGGTCTAACGTAAAAC+AGG - chr4.4:3399647-3399666 MS.gene05878:CDS 35.0%
ACTGTTCATGTTGATACTTC+AGG - chr4.4:3400185-3400204 MS.gene05878:CDS 35.0%
CAGGTTTGCAATCAAGAAAT+AGG - chr4.4:3400204-3400223 MS.gene05878:CDS 35.0%
CATAAATTGTTTGGACGGAA+AGG - chr4.4:3399780-3399799 MS.gene05878:CDS 35.0%
GGATGTGGACATAAATTGTT+TGG - chr4.4:3399771-3399790 MS.gene05878:CDS 35.0%
GTGGACATAAATTGTTTGGA+CGG - chr4.4:3399775-3399794 MS.gene05878:CDS 35.0%
GTTTGCAATCAAGAAATAGG+TGG - chr4.4:3400207-3400226 MS.gene05878:CDS 35.0%
! TGTTTTACGTTAGACCCTTT+TGG + chr4.4:3399648-3399667 None:intergenic 35.0%
!! ATTCAAATCAAGCTTGTTGC+TGG - chr4.4:3399750-3399769 MS.gene05878:CDS 35.0%
!! GAAGGTACCAAAAACCAAAA+GGG - chr4.4:3399631-3399650 MS.gene05878:CDS 35.0%
ACAACTTGGAGTTGAAGAGA+AGG - chr4.4:3399908-3399927 MS.gene05878:CDS 40.0%
CAACTTGGAGTTGAAGAGAA+GGG - chr4.4:3399909-3399928 MS.gene05878:CDS 40.0%
CAAGATGCAGAAAAATGAGG+TGG - chr4.4:3399935-3399954 MS.gene05878:CDS 40.0%
GTCAGGAAAAGCTCTTTCAT+CGG + chr4.4:3399820-3399839 None:intergenic 40.0%
TCAGGAAAAGCTCTTTCATC+GGG + chr4.4:3399819-3399838 None:intergenic 40.0%
TCAGGAAAGGTAGATTCTGT+TGG - chr4.4:3399840-3399859 MS.gene05878:CDS 40.0%
! TGGAGTATCAATCTCTTGGA+AGG - chr4.4:3399613-3399632 MS.gene05878:CDS 40.0%
!! GGAAGGTACCAAAAACCAAA+AGG - chr4.4:3399630-3399649 MS.gene05878:CDS 40.0%
!!! CGTTAGACCCTTTTGGTTTT+TGG + chr4.4:3399641-3399660 None:intergenic 40.0%
GAATCTACCTTTCCTGAGTC+AGG + chr4.4:3399837-3399856 None:intergenic 45.0%
! AGCTTTTCCTGACTCAGGAA+AGG - chr4.4:3399827-3399846 MS.gene05878:CDS 45.0%
! ATGTTTTCTTCACCACCGTC+AGG + chr4.4:3400026-3400045 None:intergenic 45.0%
! GAAAGAGCTTTTCCTGACTC+AGG - chr4.4:3399822-3399841 MS.gene05878:CDS 45.0%
!! ATCAAGCTTGTTGCTGGATG+TGG - chr4.4:3399756-3399775 MS.gene05878:CDS 45.0%
!! TACTTTTGGCTGTTTGTGCC+TGG - chr4.4:3399685-3399704 MS.gene05878:CDS 45.0%
GAAAATGGTGTTCCGCCTGA+CGG - chr4.4:3400008-3400027 MS.gene05878:CDS 50.0%
! TTTTCTTCACCACCGTCAGG+CGG + chr4.4:3400023-3400042 None:intergenic 50.0%
!! GTGGAGAGTGCCGCAAATAA+TGG - chr4.4:3399954-3399973 MS.gene05878:CDS 50.0%
AATGGTGTTCCGCCTGACGG+TGG - chr4.4:3400011-3400030 MS.gene05878:CDS 60.0%


Chromosome Type Strat End Strand Name
chr4.4 gene 3399612 3400235 3399612 ID=MS.gene05878
chr4.4 mRNA 3399612 3400235 3399612 ID=MS.gene05878.t1;Parent=MS.gene05878
chr4.4 exon 3399612 3400235 3399612 ID=MS.gene05878.t1.exon1;Parent=MS.gene05878.t1
chr4.4 CDS 3399612 3400235 3399612 ID=cds.MS.gene05878.t1;Parent=MS.gene05878.t1
Gene Sequence

>MS.gene05878

ATGGAGTATCAATCTCTTGGAAGGTACCAAAAACCAAAAGGGTCTAACGTAAAACAGGTATTGAAGATGATGCTACTTTTGGCTGTTTGTGCCTGGTTGTTATATCAAATCAAGAAGACAGAAACAAAGAAATTTGGTATTCAAATCAAGCTTGTTGCTGGATGTGGACATAAATTGTTTGGACGGAAAGGGATGCTATCACGTCCCGATGAAAGAGCTTTTCCTGACTCAGGAAAGGTAGATTCTGTTGGAGAAGTAACAAATGAGTTTAAAAGTAATGACAAAGAAAAAGAAGTACAACTTGGAGTTGAAGAGAAGGGAAACAAGATGCAGAAAAATGAGGTGGAGAGTGCCGCAAATAATGGTGTAATAGATGAGGTCCAAAGCTTTCATGATGAAAATGGTGTTCCGCCTGACGGTGGTGAAGAAAACATGTCAACTTTTAGAATAGTAAATTGGCTTAAAAAGATCAGTATTTATGAAGTCACGTATGGAGAAGATAATGATATTGAAATGAATTTGGAAGGAACGACGAATGCTACAACAGTAGACGAAGAAATTAATACAGGAACTACTGTTCATGTTGATACTTCAGGTTTGCAATCAAGAAATAGGTGGAGATAA

Protein sequence

>MS.gene05878.t1

MEYQSLGRYQKPKGSNVKQVLKMMLLLAVCAWLLYQIKKTETKKFGIQIKLVAGCGHKLFGRKGMLSRPDERAFPDSGKVDSVGEVTNEFKSNDKEKEVQLGVEEKGNKMQKNEVESAANNGVIDEVQSFHDENGVPPDGGEENMSTFRIVNWLKKISIYEVTYGEDNDIEMNLEGTTNATTVDEEINTGTTVHVDTSGLQSRNRWR