AlfalfaGEDB | MS.gene86716

M. sativa cultivar XinJiangDaYe / MS.gene86716

KEGG: -
GO: -
PFAM: -

Nr:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene86716.t1	XP_003596225.2	94.2	155	9	0	2	156	1	155	3.90E-80	307.4

Swissprot:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score

Trembl:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene86716.t1	G7IJC5	94.2	155	9	0	2	156	1	155	2.8e-80	307.4

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network:

Gene1	Gene2	correlation coefficient	p_value	FDR
MS.gene050807	MS.gene86716	0.818564	1.84E-52	-1.69E-46
MS.gene052239	MS.gene86716	-0.811981	5.33E-51	-1.69E-46
MS.gene057287	MS.gene86716	0.813999	1.93E-51	-1.69E-46

PPI:

Gene1	Gene2	Type

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene86716.t1	MTR_2g071790	94.194	155	9	0	2	156	1	155	1.69e-105	298
MS.gene86716.t1	MTR_2g071760	88.387	155	18	0	2	156	1	155	1.79e-96	275
MS.gene86716.t1	MTR_2g071750	86.452	155	21	0	2	156	1	155	2.90e-96	275
MS.gene86716.t1	MTR_2g071740	86.275	153	15	2	2	153	1	148	5.18e-90	259

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score

Find 40 sgRNAs with CRISPR-Local

Find 42 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
TGGGGTTGAGTGGGTGGATA+TGG	0.201977	2.3:+24767727	MS.gene86716:CDS
TGTGATGGTTGATCATTTAA+TGG	0.277636	2.3:+24767820	MS.gene86716:CDS
CCCAACTTGCATCATCAAAT+TGG	0.284176	2.3:-24767766	None:intergenic
TTGTGATGATGGGTTTGATA+TGG	0.302331	2.3:+24767844	MS.gene86716:CDS
TGGAGGAGGGACATGACTTT+GGG	0.323166	2.3:+24767708	MS.gene86716:CDS
AACATGCTTACAATTCCTTT+TGG	0.331685	2.3:+24767870	MS.gene86716:CDS
CATTTAATGGTTTGTGATGA+TGG	0.359988	2.3:+24767833	MS.gene86716:CDS
CCAATTTGATGATGCAAGTT+GGG	0.373282	2.3:+24767766	MS.gene86716:CDS
AAGAATATTATGATGAATCA+TGG	0.395210	2.3:+24767669	MS.gene86716:CDS
ATGGAGGAGGGACATGACTT+TGG	0.417476	2.3:+24767707	MS.gene86716:CDS
TGCTTGTGATGTGGAAATCA+AGG	0.457434	2.3:+24767484	MS.gene86716:CDS
GACATGACTTTGGGGTTGAG+TGG	0.467986	2.3:+24767717	MS.gene86716:CDS
TCCAATTTGATGATGCAAGT+TGG	0.473251	2.3:+24767765	MS.gene86716:CDS
CTATGCATGTTGAATTATCA+TGG	0.491083	2.3:-24767622	None:intergenic
ATTTAATGGTTTGTGATGAT+GGG	0.498358	2.3:+24767834	MS.gene86716:CDS
ACATGACTTTGGGGTTGAGT+GGG	0.501007	2.3:+24767718	MS.gene86716:CDS
TGAAATTAGTGAAAGCAAGA+TGG	0.502049	2.3:+24767595	MS.gene86716:CDS
TGATCTTCTCACGTCACTGA+TGG	0.502867	2.3:+24767508	MS.gene86716:CDS
CTCATAGCTATCTTGCCAAA+AGG	0.504425	2.3:-24767885	None:intergenic
TTGGCAAGATAGCTATGAGA+TGG	0.516169	2.3:+24767889	MS.gene86716:CDS
TGGCAAGATAGCTATGAGAT+GGG	0.520842	2.3:+24767890	MS.gene86716:CDS
TGACTTTGGGGTTGAGTGGG+TGG	0.527133	2.3:+24767721	MS.gene86716:CDS
TATGATGAATCATGGAACAT+AGG	0.530061	2.3:+24767677	MS.gene86716:CDS
CATGATAATTCAACATGCAT+AGG	0.547359	2.3:+24767623	MS.gene86716:CDS
GAAATTAGTGAAAGCAAGAT+GGG	0.562708	2.3:+24767596	MS.gene86716:CDS
ATGGAACATAGGACAAGTGA+TGG	0.569406	2.3:+24767688	MS.gene86716:CDS
GGAGGAGGGACATGACTTTG+GGG	0.584089	2.3:+24767709	MS.gene86716:CDS
TGGGAGGCAATTGAGTCTTG+TGG	0.585282	2.3:+24767785	MS.gene86716:CDS
CATAGGACAAGTGATGGAGG+AGG	0.595117	2.3:+24767694	MS.gene86716:CDS
ACTTGCATCATCAAATTGGA+AGG	0.605601	2.3:-24767762	None:intergenic
TGGAGATGAGAAAGATGTGA+TGG	0.610494	2.3:+24767805	MS.gene86716:CDS
GCTTGTGATGTGGAAATCAA+GGG	0.616324	2.3:+24767485	MS.gene86716:CDS
GGCAAGATAGCTATGAGATG+GGG	0.624212	2.3:+24767891	MS.gene86716:CDS
AGATAGCTATGAGATGGGGT+TGG	0.626818	2.3:+24767895	MS.gene86716:CDS
ATTTGATGATGCAAGTTGGG+AGG	0.639497	2.3:+24767769	MS.gene86716:CDS
GAACATAGGACAAGTGATGG+AGG	0.641704	2.3:+24767691	MS.gene86716:CDS
TCATCATTACTCTCATCACA+TGG	0.648258	2.3:-24767539	None:intergenic
GTGATGAGAGTAATGATGAA+AGG	0.650915	2.3:+24767543	MS.gene86716:CDS
ATAGGACAAGTGATGGAGGA+GGG	0.671083	2.3:+24767695	MS.gene86716:CDS
ATTAGTGAAAGCAAGATGGG+TGG	0.695184	2.3:+24767599	MS.gene86716:CDS

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!!	AAGAATATTATGATGAATCA+TGG	+	chr2.3:24767669-24767688	MS.gene86716:CDS	20.0%
!!!	TAGTTTAATAAGATCTTTTG+AGG	+	chr2.3:24767571-24767590	MS.gene86716:CDS	20.0%
!!	ATTTAATGGTTTGTGATGAT+GGG	+	chr2.3:24767834-24767853	MS.gene86716:CDS	25.0%
	CATGATAATTCAACATGCAT+AGG	+	chr2.3:24767623-24767642	MS.gene86716:CDS	30.0%
	CTATGCATGTTGAATTATCA+TGG	-	chr2.3:24767625-24767644	None:intergenic	30.0%
	GAAATTAGTGAAAGCAAGAT+GGG	+	chr2.3:24767596-24767615	MS.gene86716:CDS	30.0%
	TATGATGAATCATGGAACAT+AGG	+	chr2.3:24767677-24767696	MS.gene86716:CDS	30.0%
	TGAAATTAGTGAAAGCAAGA+TGG	+	chr2.3:24767595-24767614	MS.gene86716:CDS	30.0%
	TGTGATGGTTGATCATTTAA+TGG	+	chr2.3:24767820-24767839	MS.gene86716:CDS	30.0%
!	AACATGCTTACAATTCCTTT+TGG	+	chr2.3:24767870-24767889	MS.gene86716:CDS	30.0%
!	CATTTAATGGTTTGTGATGA+TGG	+	chr2.3:24767833-24767852	MS.gene86716:CDS	30.0%
	ACTTGCATCATCAAATTGGA+AGG	-	chr2.3:24767765-24767784	None:intergenic	35.0%
	GTGATGAGAGTAATGATGAA+AGG	+	chr2.3:24767543-24767562	MS.gene86716:CDS	35.0%
	TCATCATTACTCTCATCACA+TGG	-	chr2.3:24767542-24767561	None:intergenic	35.0%
!	TGATACTTTTGCTTGTGATG+TGG	+	chr2.3:24767475-24767494	MS.gene86716:CDS	35.0%
!!	CCAATTTGATGATGCAAGTT+GGG	+	chr2.3:24767766-24767785	MS.gene86716:CDS	35.0%
!!	TCCAATTTGATGATGCAAGT+TGG	+	chr2.3:24767765-24767784	MS.gene86716:CDS	35.0%
!!	TTGTGATGATGGGTTTGATA+TGG	+	chr2.3:24767844-24767863	MS.gene86716:CDS	35.0%
	ATGGAACATAGGACAAGTGA+TGG	+	chr2.3:24767688-24767707	MS.gene86716:CDS	40.0%
	ATTAGTGAAAGCAAGATGGG+TGG	+	chr2.3:24767599-24767618	MS.gene86716:CDS	40.0%
	CCCAACTTGCATCATCAAAT+TGG	-	chr2.3:24767769-24767788	None:intergenic	40.0%
	CTCATAGCTATCTTGCCAAA+AGG	-	chr2.3:24767888-24767907	None:intergenic	40.0%
	GCTTGTGATGTGGAAATCAA+GGG	+	chr2.3:24767485-24767504	MS.gene86716:CDS	40.0%
	TGCTTGTGATGTGGAAATCA+AGG	+	chr2.3:24767484-24767503	MS.gene86716:CDS	40.0%
	TGGAGATGAGAAAGATGTGA+TGG	+	chr2.3:24767805-24767824	MS.gene86716:CDS	40.0%
	TGGCAAGATAGCTATGAGAT+GGG	+	chr2.3:24767890-24767909	MS.gene86716:CDS	40.0%
	TTGGCAAGATAGCTATGAGA+TGG	+	chr2.3:24767889-24767908	MS.gene86716:CDS	40.0%
!!	ATTTGATGATGCAAGTTGGG+AGG	+	chr2.3:24767769-24767788	MS.gene86716:CDS	40.0%
	AGATAGCTATGAGATGGGGT+TGG	+	chr2.3:24767895-24767914	MS.gene86716:CDS	45.0%
	GAACATAGGACAAGTGATGG+AGG	+	chr2.3:24767691-24767710	MS.gene86716:CDS	45.0%
	GGCAAGATAGCTATGAGATG+GGG	+	chr2.3:24767891-24767910	MS.gene86716:CDS	45.0%
	TGATCTTCTCACGTCACTGA+TGG	+	chr2.3:24767508-24767527	MS.gene86716:CDS	45.0%
!	ATAGGACAAGTGATGGAGGA+GGG	+	chr2.3:24767695-24767714	MS.gene86716:CDS	45.0%
!!	ACATGACTTTGGGGTTGAGT+GGG	+	chr2.3:24767718-24767737	MS.gene86716:CDS	45.0%
	ATGGAGGAGGGACATGACTT+TGG	+	chr2.3:24767707-24767726	MS.gene86716:CDS	50.0%
	TGGAGGAGGGACATGACTTT+GGG	+	chr2.3:24767708-24767727	MS.gene86716:CDS	50.0%
	TGGGAGGCAATTGAGTCTTG+TGG	+	chr2.3:24767785-24767804	MS.gene86716:CDS	50.0%
!	CATAGGACAAGTGATGGAGG+AGG	+	chr2.3:24767694-24767713	MS.gene86716:CDS	50.0%
!!	GACATGACTTTGGGGTTGAG+TGG	+	chr2.3:24767717-24767736	MS.gene86716:CDS	50.0%
	GGAGGAGGGACATGACTTTG+GGG	+	chr2.3:24767709-24767728	MS.gene86716:CDS	55.0%
	TGGGGTTGAGTGGGTGGATA+TGG	+	chr2.3:24767727-24767746	MS.gene86716:CDS	55.0%
!!	TGACTTTGGGGTTGAGTGGG+TGG	+	chr2.3:24767721-24767740	MS.gene86716:CDS	55.0%

Chromosome	Type	Strat	End	Strand	Name
chr2.3	gene	24767455	24767925	24767455	ID=MS.gene86716
chr2.3	mRNA	24767455	24767925	24767455	ID=MS.gene86716.t1;Parent=MS.gene86716
chr2.3	exon	24767455	24767925	24767455	ID=MS.gene86716.t1.exon1;Parent=MS.gene86716.t1
chr2.3	CDS	24767455	24767925	24767455	ID=cds.MS.gene86716.t1;Parent=MS.gene86716.t1

Gene Sequence

>MS.gene86716

CAAATGGCTTCTCAAGTTTGTGATACTTTTGCTTGTGATGTGGAAATCAAGGGTGATCTTCTCACGTCACTGATGGAAGAGTTACCATGTGATGAGAGTAATGATGAAAGGTTAGATAGTTTAATAAGATCTTTTGAGGCTGAAATTAGTGAAAGCAAGATGGGTGGCCATGATAATTCAACATGCATAGGATCACAATTGAAGAGCAATTTTGAAGAATATTATGATGAATCATGGAACATAGGACAAGTGATGGAGGAGGGACATGACTTTGGGGTTGAGTGGGTGGATATGGATTTGATGACATCCTTCCAATTTGATGATGCAAGTTGGGAGGCAATTGAGTCTTGTGGAGATGAGAAAGATGTGATGGTTGATCATTTAATGGTTTGTGATGATGGGTTTGATATGGAAGAACATGCTTACAATTCCTTTTGGCAAGATAGCTATGAGATGGGGTTGGTGCATTAG

Protein sequence

>MS.gene86716.t1

QMASQVCDTFACDVEIKGDLLTSLMEELPCDESNDERLDSLIRSFEAEISESKMGGHDNSTCIGSQLKSNFEEYYDESWNIGQVMEEGHDFGVEWVDMDLMTSFQFDDASWEAIESCGDEKDVMVDHLMVCDDGFDMEEHAYNSFWQDSYEMGLVH