AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene86972

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene86972.t1 MTR_2g103620 77.644 416 48 6 23 434 8 382 0.0 550
MS.gene86972.t1 MTR_2g103640 76.984 378 55 7 28 400 3 353 9.53e-178 500
MS.gene86972.t1 MTR_5g038810 44.639 513 164 10 8 433 6 485 7.31e-112 343
MS.gene86972.t1 MTR_2g091015 53.743 374 111 8 115 430 499 868 8.70e-97 310
MS.gene86972.t1 MTR_2g091015 35.671 328 132 9 22 284 51 364 2.21e-28 118
MS.gene86972.t1 MTR_2g091015 36.207 174 91 2 114 270 449 619 3.76e-15 78.2
MS.gene86972.t1 MTR_2g091015 40.667 150 75 3 127 262 304 453 2.58e-14 75.9
MS.gene86972.t1 MTR_2g091015 32.068 237 85 6 133 295 370 604 4.34e-14 75.1
MS.gene86972.t1 MTR_2g091090 43.137 510 173 9 24 425 39 539 4.43e-96 302
MS.gene86972.t1 MTR_2g091105 41.552 580 180 13 3 433 18 587 1.86e-95 301
MS.gene86972.t1 MTR_2g091030 44.444 531 155 12 23 430 75 588 6.59e-94 295
MS.gene86972.t1 MTR_2g091285 41.468 545 175 12 23 433 58 592 6.56e-90 284
MS.gene86972.t1 MTR_2g091335 55.367 354 117 7 117 434 260 608 1.46e-87 278
MS.gene86972.t1 MTR_2g091335 37.049 305 123 11 23 277 57 342 7.83e-34 134
MS.gene86972.t1 MTR_6g045183 45.833 384 135 7 117 430 284 664 2.82e-80 263
MS.gene86972.t1 MTR_6g045183 33.117 308 130 6 17 270 75 360 2.88e-25 109
MS.gene86972.t1 MTR_5g078510 44.417 412 163 7 80 430 36 442 6.32e-80 257
MS.gene86972.t1 MTR_5g078510 33.516 182 76 3 136 272 1 182 3.44e-14 74.7
MS.gene86972.t1 MTR_2g091240 42.731 454 187 10 35 430 49 487 1.06e-78 255
MS.gene86972.t1 MTR_2g093010 48.774 367 161 9 82 433 467 821 5.25e-78 259
MS.gene86972.t1 MTR_2g093010 40.672 268 113 9 45 274 64 323 3.39e-29 121
MS.gene86972.t1 MTR_2g093010 30.952 252 139 5 40 270 208 445 3.94e-14 75.1
MS.gene86972.t1 MTR_2g093010 48.774 367 161 9 82 433 468 822 5.67e-78 259
MS.gene86972.t1 MTR_2g093010 40.672 268 113 9 45 274 65 324 3.43e-29 121
MS.gene86972.t1 MTR_2g093010 30.952 252 139 5 40 270 209 446 3.94e-14 75.1
MS.gene86972.t1 MTR_2g091245 49.408 338 122 6 128 433 241 561 9.53e-74 244
MS.gene86972.t1 MTR_2g091250 47.028 387 154 7 90 433 816 1194 2.19e-71 244
MS.gene86972.t1 MTR_2g091250 38.629 321 116 8 24 270 52 365 1.75e-42 160
MS.gene86972.t1 MTR_2g091250 40.110 182 88 6 103 270 258 432 2.55e-18 88.2
MS.gene86972.t1 MTR_2g091250 36.649 191 86 3 115 270 480 670 2.47e-17 85.1
MS.gene86972.t1 MTR_2g091250 39.355 155 77 2 133 270 656 810 1.61e-16 82.8
MS.gene86972.t1 MTR_2g091250 37.143 210 104 5 82 270 370 572 1.65e-16 82.8
MS.gene86972.t1 MTR_2g091250 36.879 141 86 1 133 270 586 726 3.20e-16 81.6
MS.gene86972.t1 MTR_2g091250 32.569 218 111 4 112 293 365 582 7.74e-15 77.4
MS.gene86972.t1 MTR_2g091250 34.010 197 68 4 133 270 337 530 4.26e-14 75.1
MS.gene86972.t1 MTR_2g091250 31.858 226 138 4 55 268 643 864 4.37e-12 68.9
MS.gene86972.t1 MTR_2g104190 49.835 303 116 5 136 429 332 607 1.57e-69 232
MS.gene86972.t1 MTR_2g104190 32.432 296 144 9 14 277 66 337 8.80e-18 86.3
MS.gene86972.t1 MTR_2g010420 43.143 350 148 7 129 434 399 741 1.65e-69 234
MS.gene86972.t1 MTR_2g010420 31.122 196 111 4 99 270 278 473 1.57e-12 70.1
MS.gene86972.t1 MTR_2g091025 37.787 479 189 11 22 433 82 518 2.27e-68 228
MS.gene86972.t1 MTR_2g091080 45.522 402 160 8 82 434 371 762 4.12e-67 228
MS.gene86972.t1 MTR_2g091080 38.148 270 124 12 27 271 29 280 1.03e-23 104
MS.gene86972.t1 MTR_2g091080 35.749 207 113 5 75 270 222 419 1.33e-17 85.9
MS.gene86972.t1 MTR_2g091260 50.000 336 120 6 133 430 448 773 6.81e-67 229
MS.gene86972.t1 MTR_2g091260 36.306 314 143 8 20 286 78 381 2.60e-36 142
MS.gene86972.t1 MTR_2g091260 38.406 138 74 1 133 270 280 406 8.82e-14 73.9
MS.gene86972.t1 MTR_2g091260 37.748 151 91 1 133 280 336 486 5.02e-13 71.6
MS.gene86972.t1 MTR_2g010420 42.407 349 150 7 129 433 399 740 4.82e-66 226
MS.gene86972.t1 MTR_2g010420 33.136 169 92 3 123 270 305 473 1.66e-12 70.1
MS.gene86972.t1 MTR_3g110385 47.188 320 137 6 133 433 426 732 2.82e-62 216
MS.gene86972.t1 MTR_3g110385 44.853 136 71 1 135 270 211 342 8.88e-19 89.4
MS.gene86972.t1 MTR_3g110385 35.359 181 99 3 133 295 300 480 6.45e-15 77.4
MS.gene86972.t1 MTR_2g091290 40.102 394 192 8 78 433 350 737 2.15e-61 213
MS.gene86972.t1 MTR_2g091290 37.868 272 129 8 22 271 52 305 1.58e-26 113
MS.gene86972.t1 MTR_2g091290 33.898 177 64 3 133 267 276 441 8.00e-13 70.9
MS.gene86972.t1 MTR_2g091370 39.305 374 152 7 129 433 372 739 7.02e-60 209
MS.gene86972.t1 MTR_2g091370 38.095 294 133 13 12 271 57 335 6.83e-25 108
MS.gene86972.t1 MTR_2g091370 27.341 267 146 6 49 267 205 471 2.28e-11 66.2
MS.gene86972.t1 MTR_2g091040 35.234 491 185 11 24 392 39 518 2.11e-56 194
MS.gene86972.t1 MTR_2g091110 41.093 421 177 9 78 433 787 1201 1.12e-55 199
MS.gene86972.t1 MTR_2g091110 37.915 211 103 5 81 270 650 853 4.18e-23 103
MS.gene86972.t1 MTR_2g091110 40.957 188 97 4 82 255 749 936 9.00e-22 99.0
MS.gene86972.t1 MTR_2g091110 41.935 155 66 3 133 270 468 615 2.55e-18 88.2
MS.gene86972.t1 MTR_2g091110 39.456 147 72 2 133 262 629 775 6.09e-18 87.0
MS.gene86972.t1 MTR_2g091110 37.438 203 106 6 82 270 462 657 1.48e-15 79.7
MS.gene86972.t1 MTR_2g091110 38.415 164 89 3 115 270 365 524 2.22e-15 79.3
MS.gene86972.t1 MTR_2g091110 50.538 93 36 3 44 136 43 125 7.25e-14 74.3
MS.gene86972.t1 MTR_2g091110 39.189 148 65 3 130 270 556 685 3.10e-12 69.3
MS.gene86972.t1 MTR_2g090980 54.717 212 80 3 232 430 308 516 1.47e-54 190
MS.gene86972.t1 MTR_8g106540 41.231 325 120 7 112 434 33 288 7.02e-54 182
MS.gene86972.t1 MTR_8g107320 52.336 214 77 5 222 434 24 213 2.80e-52 175
MS.gene86972.t1 MTR_5g078500 38.346 266 119 5 24 251 40 298 8.58e-38 139
MS.gene86972.t1 MTR_2g091325 36.111 324 122 9 27 270 82 400 2.49e-35 138
MS.gene86972.t1 MTR_2g091325 50.543 184 63 5 115 270 364 547 3.13e-32 129
MS.gene86972.t1 MTR_2g091325 36.283 226 112 6 82 288 296 508 1.29e-20 94.7
MS.gene86972.t1 MTR_2g091325 38.514 148 74 3 133 270 288 428 2.00e-12 69.3
MS.gene86972.t1 MTR_2g091070 59.542 131 50 1 300 430 21 148 1.35e-33 125
MS.gene86972.t1 MTR_7g056313 35.220 318 131 8 22 283 35 333 5.13e-33 128
MS.gene86972.t1 MTR_7g056313 35.484 155 83 2 133 270 214 368 3.13e-13 71.2
MS.gene86972.t1 MTR_7g056313 37.500 168 76 4 115 257 206 369 5.30e-13 70.5
MS.gene86972.t1 MTR_2g091330 37.013 308 137 8 26 286 79 376 7.40e-29 119
MS.gene86972.t1 MTR_2g091330 39.877 163 74 3 135 273 431 593 5.15e-16 80.9
MS.gene86972.t1 MTR_2g091330 40.123 162 82 2 111 268 319 469 1.12e-12 70.5
MS.gene86972.t1 MTR_2g091330 43.478 92 52 0 133 224 492 583 1.84e-11 66.6
MS.gene86972.t1 MTR_8g099800 70.732 82 22 2 97 178 8 87 1.17e-27 106
MS.gene86972.t1 MTR_2g091010 36.066 305 146 12 2 272 51 340 1.54e-27 115
MS.gene86972.t1 MTR_2g091010 37.200 250 112 5 81 292 373 615 1.36e-19 92.0
MS.gene86972.t1 MTR_2g091010 40.936 171 72 3 115 267 316 475 1.99e-19 91.3
MS.gene86972.t1 MTR_8g467200 35.294 289 159 8 152 430 32 302 1.52e-24 105
MS.gene86972.t1 MTR_2g091280 41.989 181 60 2 135 270 531 711 9.20e-24 104
MS.gene86972.t1 MTR_2g091280 37.037 297 128 8 23 270 59 345 1.14e-22 101
MS.gene86972.t1 MTR_2g091280 41.722 151 81 2 127 270 307 457 9.74e-18 86.3
MS.gene86972.t1 MTR_2g091280 35.443 237 95 6 92 270 321 557 3.72e-16 81.3
MS.gene86972.t1 MTR_2g091280 33.488 215 91 5 115 277 407 621 8.23e-12 67.8
MS.gene86972.t1 MTR_4g084920 32.000 325 156 11 106 395 10 304 1.97e-20 92.0
MS.gene86972.t1 MTR_4g046660 47.143 140 65 3 296 433 447 579 5.63e-20 93.2
MS.gene86972.t1 MTR_2g091315 33.333 282 132 9 27 270 49 312 1.68e-18 88.2
MS.gene86972.t1 MTR_2g091315 29.771 262 129 4 40 270 201 438 9.85e-13 70.5
MS.gene86972.t1 MTR_2g091360 52.174 92 44 0 133 224 65 156 5.54e-18 84.7
MS.gene86972.t1 MTR_2g091005 30.796 289 146 9 9 267 39 303 5.85e-18 86.7
MS.gene86972.t1 MTR_2g090640 41.259 143 73 1 133 275 197 328 5.62e-17 83.6
MS.gene86972.t1 MTR_2g090640 38.462 169 77 4 117 269 261 418 7.29e-13 70.9
MS.gene86972.t1 MTR_7g056317 66.667 87 25 2 344 430 1 83 1.78e-15 72.8
MS.gene86972.t1 MTR_4g095080 26.284 331 182 16 152 433 25 342 4.26e-11 65.1
MS.gene86972.t1 MTR_2g064120 55.405 74 32 1 51 123 132 205 7.98e-11 62.8
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene86972.t1 AT5G27220 30.448 335 192 13 99 425 497 798 2.68e-14 75.9
MS.gene86972.t1 AT5G27220 30.448 335 192 13 99 425 497 798 2.68e-14 75.9
MS.gene86972.t1 AT5G27220 30.448 335 192 13 99 425 497 798 2.94e-14 75.9
MS.gene86972.t1 AT5G27220 30.448 335 192 13 99 425 497 798 2.94e-14 75.9

Find 64 sgRNAs with CRISPR-Local

Find 112 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CTTCTCAATATTTCTCTTTA+TGG 0.075175 2.3:-396655 None:intergenic
CTTCGTTGAATATGAATAAA+AGG 0.247349 2.3:+396517 MS.gene86972:CDS
AGAAAATACTGAAAATTCTT+TGG 0.251631 2.3:+397751 MS.gene86972:CDS
AGATCCAGCAAAACTTGTTT+TGG 0.272394 2.3:+397556 MS.gene86972:CDS
ACAGCTTTGTCTCCCTTCTT+AGG 0.285668 2.3:-397608 None:intergenic
CTGCAAAGAGCTAGAAAATA+AGG 0.286190 2.3:+396680 MS.gene86972:CDS
GAGTCAAAGCTGAATAATTT+TGG 0.290126 2.3:+397050 MS.gene86972:CDS
CTTACACAAGATTTAATATC+TGG 0.303638 2.3:-397683 None:intergenic
GATGGAACAAGTGAGGAATT+TGG 0.315869 2.3:+397506 MS.gene86972:CDS
CAGACTCAAGTTCCTTAACT+TGG 0.324284 2.3:-396707 None:intergenic
AGATAGCTGTAAAGCTGTTT+AGG 0.328283 2.3:+397882 MS.gene86972:CDS
TCAAAAGAGGAGGAATTTAA+AGG 0.372635 2.3:+396894 MS.gene86972:CDS
CTTGGAAAGCAACAATTCCT+TGG 0.374250 2.3:+396428 MS.gene86972:CDS
CAATTCAGTATCACCATTGA+TGG 0.374340 2.3:+397488 MS.gene86972:CDS
CTGTCAAATAACCTCTTAAC+TGG 0.388338 2.3:-396396 None:intergenic
TTATCGATGGCGACGATGTC+AGG 0.391493 2.3:+395944 None:intergenic
GAAAATTCTTTGGAGGTTCT+CGG 0.397484 2.3:+397761 MS.gene86972:CDS
TTCCTCACTTGTTCCATCAA+TGG 0.405596 2.3:-397501 None:intergenic
TTGTCTCCCTTCTTAGGCAG+TGG 0.411661 2.3:-397602 None:intergenic
TTCTTAGGCAGTGGAATCGT+GGG 0.412112 2.3:-397593 None:intergenic
TCTGAAAAGAATATACTTGA+TGG 0.418087 2.3:+396726 MS.gene86972:CDS
GAAGGAAGAAGTAGATCAAA+AGG 0.433938 2.3:+396256 MS.gene86972:CDS
TTGGATTTAACAGCACGAAC+TGG 0.436687 2.3:+396212 MS.gene86972:intron
CTTTGAGATTGTCCTCAGCT+TGG 0.447230 2.3:-396980 None:intergenic
AAATAAGGAGACCCAAGTTA+AGG 0.448359 2.3:+396695 MS.gene86972:CDS
ATTTGACAGCAACAATTCCT+TGG 0.450325 2.3:+396410 MS.gene86972:CDS
CAAGGAATTGTTGCTTTCCA+AGG 0.451728 2.3:-396427 None:intergenic
ACATTTCGCGAGCCGACAAA+AGG 0.460184 2.3:+396821 MS.gene86972:CDS
GGAATTTGAATCAAAAGAAA+AGG 0.461737 2.3:+396842 MS.gene86972:CDS
CTACTGCACTGCCAGTTAAG+AGG 0.461854 2.3:+396385 MS.gene86972:CDS
CACGATTCCACTGCCTAAGA+AGG 0.465950 2.3:+397595 MS.gene86972:CDS
TCAGATTGAAGTACAAATCA+AGG 0.469530 2.3:+397022 MS.gene86972:CDS
CTGATTCAAAGTTCTTCACT+AGG 0.474701 2.3:-396791 None:intergenic
AAATTATTCAGCTTTGACTC+AGG 0.479176 2.3:-397047 None:intergenic
GTAAAGCTGTTTAGGACCCT+GGG 0.479502 2.3:+397890 MS.gene86972:CDS
AAATACTGAAAATTCTTTGG+AGG 0.482682 2.3:+397754 MS.gene86972:CDS
TGTAAAGCTGTTTAGGACCC+TGG 0.484740 2.3:+397889 MS.gene86972:CDS
AATCATTTGAGGAATGCAAC+GGG 0.489095 2.3:+396601 MS.gene86972:CDS
AGTGTTTGAAGCAAAGGAAG+AGG 0.504885 2.3:+396926 MS.gene86972:CDS
GAATTATCAGCTGATTTGCC+TGG 0.507013 2.3:-396540 None:intergenic
GGAATTTGAAAGAAGAGTGA+AGG 0.507055 2.3:+396863 MS.gene86972:CDS
TTGAATATGAATAAAAGGCC+AGG 0.518437 2.3:+396522 MS.gene86972:CDS
AGACTCAAGTTCCTTAACTT+GGG 0.522775 2.3:-396706 None:intergenic
CACGAACTGGACAGCCAAGT+GGG 0.524603 2.3:+396225 MS.gene86972:CDS
CTTCTTAGGCAGTGGAATCG+TGG 0.525924 2.3:-397594 None:intergenic
AAATAGAAGAGTGGCGATTG+TGG 0.559419 2.3:+396631 MS.gene86972:CDS
GAAGGAGTTCCAGTCAAAAG+AGG 0.567276 2.3:+396881 MS.gene86972:CDS
AGTGAAAGTGTTTGAAGCAA+AGG 0.574401 2.3:+396920 MS.gene86972:CDS
TTTCTTAACAGAAAATTCCA+AGG 0.575654 2.3:-396445 None:intergenic
AAATCATTTGAGGAATGCAA+CGG 0.576495 2.3:+396600 MS.gene86972:CDS
ACGATTCCACTGCCTAAGAA+GGG 0.594525 2.3:+397596 MS.gene86972:CDS
ATATGCAGATACATCTAACA+TGG 0.600498 2.3:+397460 MS.gene86972:intron
GGAGTTCCAGTCAAAAGAGG+AGG 0.625446 2.3:+396884 MS.gene86972:CDS
ACGAACTGGACAGCCAAGTG+GGG 0.626248 2.3:+396226 MS.gene86972:CDS
GACTTTATGTACAAAACCCA+GGG 0.629177 2.3:-397906 None:intergenic
GCAAAGGAAGAGGAATTCGA+AGG 0.634363 2.3:+396936 MS.gene86972:CDS
CACCATTGATGGAACAAGTG+AGG 0.634662 2.3:+397499 MS.gene86972:CDS
TGCAAGTTCTTTCCTCAGCG+AGG 0.638740 2.3:-396568 None:intergenic
AGACTTTATGTACAAAACCC+AGG 0.639555 2.3:-397907 None:intergenic
GGAATCAACAAATAGAAGAG+TGG 0.642926 2.3:+396622 MS.gene86972:CDS
GCACGAACTGGACAGCCAAG+TGG 0.645283 2.3:+396224 MS.gene86972:intron
GCAATTTGAAAACCAAGCTG+AGG 0.662165 2.3:+396968 MS.gene86972:CDS
CGAACTGGACAGCCAAGTGG+GGG 0.663711 2.3:+396227 MS.gene86972:CDS
TCTTAGGCAGTGGAATCGTG+GGG 0.670339 2.3:-397592 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AACATATATATTATGATAAA+CGG - chr2.3:396183-396202 None:intergenic 10.0%
!! TATATATGTTATAGATTCTT+TGG + chr2.3:396193-396212 MS.gene86972:intron 15.0%
!! AATGAAATAAGATGATTATC+AGG - chr2.3:396052-396071 None:intergenic 20.0%
!! AGAAAATACTGAAAATTCTT+TGG + chr2.3:397751-397770 MS.gene86972:CDS 20.0%
!! ATTGTTGAAAAAATCATTTG+AGG + chr2.3:396590-396609 MS.gene86972:CDS 20.0%
!! TAAATTCTGTAGTTAAATGA+TGG + chr2.3:396332-396351 MS.gene86972:intron 20.0%
!!! ATTTTCTGTTAAGAAATCAA+AGG + chr2.3:396452-396471 MS.gene86972:CDS 20.0%
! AAATACTGAAAATTCTTTGG+AGG + chr2.3:397754-397773 MS.gene86972:CDS 25.0%
! AAATGAGCTTGAATCAAAAA+AGG + chr2.3:396755-396774 MS.gene86972:CDS 25.0%
! AAGGATAAACATTCGATAAA+TGG - chr2.3:396109-396128 None:intergenic 25.0%
! AATCAGGATAGCATATAATA+GGG + chr2.3:395975-395994 MS.gene86972:intron 25.0%
! AATGAGCTTGAATCAAAAAA+GGG + chr2.3:396756-396775 MS.gene86972:CDS 25.0%
! ACATAAGCAACAAAAATTGA+AGG - chr2.3:397404-397423 None:intergenic 25.0%
! ATAGAAACTTAGTGAATCAT+TGG - chr2.3:397303-397322 None:intergenic 25.0%
! CTTACACAAGATTTAATATC+TGG - chr2.3:397686-397705 None:intergenic 25.0%
! CTTCGTTGAATATGAATAAA+AGG + chr2.3:396517-396536 MS.gene86972:CDS 25.0%
! CTTCTCAATATTTCTCTTTA+TGG - chr2.3:396658-396677 None:intergenic 25.0%
! GGAATTTGAATCAAAAGAAA+AGG + chr2.3:396842-396861 MS.gene86972:CDS 25.0%
! TACGATGAAGAAAAAGAATT+TGG + chr2.3:397110-397129 MS.gene86972:CDS 25.0%
! TCTGAAAAGAATATACTTGA+TGG + chr2.3:396726-396745 MS.gene86972:CDS 25.0%
! TTAAGATGGCAAAAAATTGA+AGG - chr2.3:396128-396147 None:intergenic 25.0%
! TTATAGCCAAAACTACAAAT+AGG - chr2.3:397258-397277 None:intergenic 25.0%
! TTATCAGGATTTAAAGAAAC+AGG - chr2.3:396037-396056 None:intergenic 25.0%
! TTTCTTAACAGAAAATTCCA+AGG - chr2.3:396448-396467 None:intergenic 25.0%
!! AATAGTCCTATTTGTAGTTT+TGG + chr2.3:397249-397268 MS.gene86972:intron 25.0%
!! ATATATACTATGAAAGCATG+TGG - chr2.3:397360-397379 None:intergenic 25.0%
!! CATTTCAACAACAACTTTTA+TGG + chr2.3:397170-397189 MS.gene86972:intron 25.0%
!! CTTTATTTCTTCAGTGAATA+AGG + chr2.3:396298-396317 MS.gene86972:intron 25.0%
!! CTTTTATTCATATTCAACGA+AGG - chr2.3:396519-396538 None:intergenic 25.0%
!! TTGATTCAAATTCCTTTTGT+CGG - chr2.3:396836-396855 None:intergenic 25.0%
AAATCATTTGAGGAATGCAA+CGG + chr2.3:396600-396619 MS.gene86972:CDS 30.0%
ATATGCAGATACATCTAACA+TGG + chr2.3:397460-397479 MS.gene86972:intron 30.0%
ATTATGATAAACGGTACAAC+AGG - chr2.3:396174-396193 None:intergenic 30.0%
CAAAAATTGAAGGTGACAAT+AGG - chr2.3:397394-397413 None:intergenic 30.0%
CAATCAGGATAGCATATAAT+AGG + chr2.3:395974-395993 MS.gene86972:intron 30.0%
TAATGCTTCTGTTTAGAAGA+TGG + chr2.3:396359-396378 MS.gene86972:intron 30.0%
TATGTGTGTGATATAGAAAC+TGG - chr2.3:397230-397249 None:intergenic 30.0%
TCAAAAAAGGGTGAATTTGA+TGG + chr2.3:396768-396787 MS.gene86972:CDS 30.0%
TCAAAAGAGGAGGAATTTAA+AGG + chr2.3:396894-396913 MS.gene86972:CDS 30.0%
TCAGATTGAAGTACAAATCA+AGG + chr2.3:397022-397041 MS.gene86972:CDS 30.0%
TTATGATAAACGGTACAACA+GGG - chr2.3:396173-396192 None:intergenic 30.0%
TTGAATATGAATAAAAGGCC+AGG + chr2.3:396522-396541 MS.gene86972:CDS 30.0%
! AAATTATTCAGCTTTGACTC+AGG - chr2.3:397050-397069 None:intergenic 30.0%
! ATATCCAAAACAAGTTTTGC+TGG - chr2.3:397563-397582 None:intergenic 30.0%
! CAATCTGATTCTCTTTTAAC+TGG - chr2.3:397010-397029 None:intergenic 30.0%
! GAGTCAAAGCTGAATAATTT+TGG + chr2.3:397050-397069 MS.gene86972:CDS 30.0%
! TCAAAGCTGAATAATTTTGG+TGG + chr2.3:397053-397072 MS.gene86972:CDS 30.0%
! TTTGCCATCTTAATGCTTTT+GGG + chr2.3:396135-396154 MS.gene86972:intron 30.0%
! TTTTGCCATCTTAATGCTTT+TGG + chr2.3:396134-396153 MS.gene86972:intron 30.0%
!! TAATTTTGGTGGACAACTAA+AGG + chr2.3:397064-397083 MS.gene86972:CDS 30.0%
!! TATTCCCAAAAGCATTAAGA+TGG - chr2.3:396142-396161 None:intergenic 30.0%
!! TTAATGCTTTTGGGAATATG+CGG + chr2.3:396144-396163 MS.gene86972:intron 30.0%
!!! CTGATGATTTTGTCGATTTA+TGG + chr2.3:397788-397807 MS.gene86972:CDS 30.0%
AAATAAGGAGACCCAAGTTA+AGG + chr2.3:396695-396714 MS.gene86972:CDS 35.0%
AATCATTTGAGGAATGCAAC+GGG + chr2.3:396601-396620 MS.gene86972:CDS 35.0%
AGACTCAAGTTCCTTAACTT+GGG - chr2.3:396709-396728 None:intergenic 35.0%
AGACTTTATGTACAAAACCC+AGG - chr2.3:397910-397929 None:intergenic 35.0%
AGATAGCTGTAAAGCTGTTT+AGG + chr2.3:397882-397901 MS.gene86972:CDS 35.0%
AGTGCAAAAATCAAGGTTCA+CGG - chr2.3:396011-396030 None:intergenic 35.0%
ATTTGACAGCAACAATTCCT+TGG + chr2.3:396410-396429 MS.gene86972:CDS 35.0%
CAATTCAGTATCACCATTGA+TGG + chr2.3:397488-397507 MS.gene86972:CDS 35.0%
CAGGATAAGTGCAAAAATCA+AGG - chr2.3:396018-396037 None:intergenic 35.0%
CTGATTCAAAGTTCTTCACT+AGG - chr2.3:396794-396813 None:intergenic 35.0%
CTGCAAAGAGCTAGAAAATA+AGG + chr2.3:396680-396699 MS.gene86972:CDS 35.0%
CTGTCAAATAACCTCTTAAC+TGG - chr2.3:396399-396418 None:intergenic 35.0%
GAAGGAAGAAGTAGATCAAA+AGG + chr2.3:396256-396275 MS.gene86972:CDS 35.0%
GACTTTATGTACAAAACCCA+GGG - chr2.3:397909-397928 None:intergenic 35.0%
GGAATCAACAAATAGAAGAG+TGG + chr2.3:396622-396641 MS.gene86972:CDS 35.0%
GGAATTTGAAAGAAGAGTGA+AGG + chr2.3:396863-396882 MS.gene86972:CDS 35.0%
! ATAATTCATTTTCCTCGCTG+AGG + chr2.3:396556-396575 MS.gene86972:CDS 35.0%
! GAAAATTCTTTGGAGGTTCT+CGG + chr2.3:397761-397780 MS.gene86972:CDS 35.0%
! TAAATTCCTCCTCTTTTGAC+TGG - chr2.3:396893-396912 None:intergenic 35.0%
!! AGATCCAGCAAAACTTGTTT+TGG + chr2.3:397556-397575 MS.gene86972:CDS 35.0%
!! AGTGAAAGTGTTTGAAGCAA+AGG + chr2.3:396920-396939 MS.gene86972:CDS 35.0%
!! ATGTCAGGTTAGTTTCAATC+AGG + chr2.3:395959-395978 MS.gene86972:intron 35.0%
!! GGTTTTGTACATAAAGTCTC+TGG + chr2.3:397911-397930 MS.gene86972:CDS 35.0%
!! GTTTTTCTAGCAGATAGATG+CGG - chr2.3:397649-397668 None:intergenic 35.0%
AAATAGAAGAGTGGCGATTG+TGG + chr2.3:396631-396650 MS.gene86972:CDS 40.0%
CAGACTCAAGTTCCTTAACT+TGG - chr2.3:396710-396729 None:intergenic 40.0%
CTTGGAAAGCAACAATTCCT+TGG + chr2.3:396428-396447 MS.gene86972:CDS 40.0%
GATGGAACAAGTGAGGAATT+TGG + chr2.3:397506-397525 MS.gene86972:CDS 40.0%
GCAATTTGAAAACCAAGCTG+AGG + chr2.3:396968-396987 MS.gene86972:CDS 40.0%
TTCCTCACTTGTTCCATCAA+TGG - chr2.3:397504-397523 None:intergenic 40.0%
TTGGATTTAACAGCACGAAC+TGG + chr2.3:396212-396231 MS.gene86972:intron 40.0%
! AGTGTTTGAAGCAAAGGAAG+AGG + chr2.3:396926-396945 MS.gene86972:CDS 40.0%
!! CAAGGAATTGTTGCTTTCCA+AGG - chr2.3:396430-396449 None:intergenic 40.0%
!! GAATTATCAGCTGATTTGCC+TGG - chr2.3:396543-396562 None:intergenic 40.0%
!!! ACTGGACTAGCTTTTCAACT+TGG - chr2.3:397212-397231 None:intergenic 40.0%
ACGATTCCACTGCCTAAGAA+GGG + chr2.3:397596-397615 MS.gene86972:CDS 45.0%
CACCATTGATGGAACAAGTG+AGG + chr2.3:397499-397518 MS.gene86972:CDS 45.0%
GAAGGAGTTCCAGTCAAAAG+AGG + chr2.3:396881-396900 MS.gene86972:CDS 45.0%
GCAAAGGAAGAGGAATTCGA+AGG + chr2.3:396936-396955 MS.gene86972:CDS 45.0%
TTCTTAGGCAGTGGAATCGT+GGG - chr2.3:397596-397615 None:intergenic 45.0%
! ACAGCTTTGTCTCCCTTCTT+AGG - chr2.3:397611-397630 None:intergenic 45.0%
! GTAAAGCTGTTTAGGACCCT+GGG + chr2.3:397890-397909 MS.gene86972:CDS 45.0%
! TGTAAAGCTGTTTAGGACCC+TGG + chr2.3:397889-397908 MS.gene86972:CDS 45.0%
!! CTTTGAGATTGTCCTCAGCT+TGG - chr2.3:396983-397002 None:intergenic 45.0%
ACATTTCGCGAGCCGACAAA+AGG + chr2.3:396821-396840 MS.gene86972:CDS 50.0%
CACGATTCCACTGCCTAAGA+AGG + chr2.3:397595-397614 MS.gene86972:CDS 50.0%
CTACTGCACTGCCAGTTAAG+AGG + chr2.3:396385-396404 MS.gene86972:CDS 50.0%
CTTCTTAGGCAGTGGAATCG+TGG - chr2.3:397597-397616 None:intergenic 50.0%
GGAGTTCCAGTCAAAAGAGG+AGG + chr2.3:396884-396903 MS.gene86972:CDS 50.0%
TCTTAGGCAGTGGAATCGTG+GGG - chr2.3:397595-397614 None:intergenic 50.0%
TGCAAGTTCTTTCCTCAGCG+AGG - chr2.3:396571-396590 None:intergenic 50.0%
TTGTCTCCCTTCTTAGGCAG+TGG - chr2.3:397605-397624 None:intergenic 50.0%
ACGAACTGGACAGCCAAGTG+GGG + chr2.3:396226-396245 MS.gene86972:CDS 55.0%
CACGAACTGGACAGCCAAGT+GGG + chr2.3:396225-396244 MS.gene86972:CDS 55.0%
TCCTTCCAATCACCCCCACT+TGG - chr2.3:396242-396261 None:intergenic 55.0%
CGAACTGGACAGCCAAGTGG+GGG + chr2.3:396227-396246 MS.gene86972:CDS 60.0%
GACAGCCAAGTGGGGGTGAT+TGG + chr2.3:396234-396253 MS.gene86972:CDS 60.0%
GCACGAACTGGACAGCCAAG+TGG + chr2.3:396224-396243 MS.gene86972:intron 60.0%
GCCAAGTGGGGGTGATTGGA+AGG + chr2.3:396238-396257 MS.gene86972:CDS 60.0%


Chromosome Type Strat End Strand Name
chr2.3 gene 395950 397946 395950 ID=MS.gene86972
chr2.3 mRNA 395950 397946 395950 ID=MS.gene86972.t1;Parent=MS.gene86972
chr2.3 exon 395950 395965 395950 ID=MS.gene86972.t1.exon1;Parent=MS.gene86972.t1
chr2.3 CDS 395950 395965 395950 ID=cds.MS.gene86972.t1;Parent=MS.gene86972.t1
chr2.3 exon 396225 396277 396225 ID=MS.gene86972.t1.exon2;Parent=MS.gene86972.t1
chr2.3 CDS 396225 396277 396225 ID=cds.MS.gene86972.t1;Parent=MS.gene86972.t1
chr2.3 exon 396375 397131 396375 ID=MS.gene86972.t1.exon3;Parent=MS.gene86972.t1
chr2.3 CDS 396375 397131 396375 ID=cds.MS.gene86972.t1;Parent=MS.gene86972.t1
chr2.3 exon 397468 397946 397468 ID=MS.gene86972.t1.exon4;Parent=MS.gene86972.t1
chr2.3 CDS 397468 397946 397468 ID=cds.MS.gene86972.t1;Parent=MS.gene86972.t1
Gene Sequence

>MS.gene86972

ATGGCGACGATGTCAGGTTAGTTTCAATCAGGATAGCATATAATAGGGTTTTGATAAACCGTGAACCTTGATTTTTGCACTTATCCTGTTTCTTTAAATCCTGATAATCATCTTATTTCATTTAAAGAAAAATTGAGACTGTTGTCTTTATCGTTTCCATTTATCGAATGTTTATCCTTCAATTTTTTGCCATCTTAATGCTTTTGGGAATATGCGGTATCCCTGTTGTACCGTTTATCATAATATATATGTTATAGATTCTTTGGATTTAACAGCACGAACTGGACAGCCAAGTGGGGGTGATTGGAAGGAAGAAGTAGATCAAAAGGTAATATGCTATTTTCTCATCTTTATTTCTTCAGTGAATAAGGTTGAAAAACAATAAATTCTGTAGTTAAATGATGGATTTTAATGCTTCTGTTTAGAAGATGGATACTACTGCACTGCCAGTTAAGAGGTTATTTGACAGCAACAATTCCTTGGAAAGCAACAATTCCTTGGAATTTTCTGTTAAGAAATCAAAGGTGTCACTATACGATGATCGTGACAAAGATGAAGACGATATTCCTTCGTTGAATATGAATAAAAGGCCAGGCAAATCAGCTGATAATTCATTTTCCTCGCTGAGGAAAGAACTTGCATTGTTGAAAAAATCATTTGAGGAATGCAACGGGAATCAACAAATAGAAGAGTGGCGATTGTGGTCCATAAAGAGAAATATTGAGAAGTGCTGCAAAGAGCTAGAAAATAAGGAGACCCAAGTTAAGGAACTTGAGTCTGAAAAGAATATACTTGATGGTCGAGTAAATGAGCTTGAATCAAAAAAGGGTGAATTTGATGGCCTAGTGAAGAACTTTGAATCAGAAAAGACACATTTCGCGAGCCGACAAAAGGAATTTGAATCAAAAGAAAAGGAATTTGAAAGAAGAGTGAAGGAGTTCCAGTCAAAAGAGGAGGAATTTAAAGGTCGAGTGAAAGTGTTTGAAGCAAAGGAAGAGGAATTCGAAGGAAAAATGCAGCAATTTGAAAACCAAGCTGAGGACAATCTCAAAGCACTCCAGTTAAAAGAGAATCAGATTGAAGTACAAATCAAGGATCCTGAGTCAAAGCTGAATAATTTTGGTGGACAACTAAAGGAGCTTGAGTTGACAGAGAAACAATACGATGAAGAAAAAGAATTTGGTAAATTTTGCTATTCATCTATTTTATTGAAAGAATTTCATTTCAACAACAACTTTTATGGATGCAGTACATCTTAACCAAGTTGAAAAGCTAGTCCAGTTTCTATATCACACACATAATAGTCCTATTTGTAGTTTTGGCTATAATATTAATCAATTAAACACTACTCCAATGATTCACTAAGTTTCTATATTGACATTGAAACTGTTAGTATAATGTTTTTCTCCACATGCTTTCATAGTATATATCTTTTTTAATTCCTATTGTCACCTTCAATTTTTGTTGCTTATGTAAATATGATCAGAGAGCTTGACTATCTGCTATCTAAATATGCAGATACATCTAACATGGATGATCAATTCAGTATCACCATTGATGGAACAAGTGAGGAATTTGGCATTCTAGATAATTTGCGAAAATCATCAGATCCAGCAAAACTTGTTTTGGATATAATACTAAACCCCACGATTCCACTGCCTAAGAAGGGAGACAAAGCTGTGATTATTGATGAAAGCCGCATCTATCTGCTAGAAAAACTGATGATAATCTCACCAGATATTAAATCTTGTGTAAGAGATGAAGCTTTGAAGCTAGCACGTGAATTGAAAGCTAACATGAAAGAAAATACTGAAAATTCTTTGGAGGTTCTCGGATTTCTGATGATTTTGTCGATTTATGGATTGCTTACTTACTTTGATGAAGATGAAGTCTTCAAGCTTTTTGCTTCTGTTGCGTCTGTTGCTGAATACAAGATAGCTGTAAAGCTGTTTAGGACCCTGGGTTTTGTACATAAAGTCTCTGGTATGTTGATTTAG
Protein sequence

>MS.gene86972.t1

MATMSARTGQPSGGDWKEEVDQKKMDTTALPVKRLFDSNNSLESNNSLEFSVKKSKVSLYDDRDKDEDDIPSLNMNKRPGKSADNSFSSLRKELALLKKSFEECNGNQQIEEWRLWSIKRNIEKCCKELENKETQVKELESEKNILDGRVNELESKKGEFDGLVKNFESEKTHFASRQKEFESKEKEFERRVKEFQSKEEEFKGRVKVFEAKEEEFEGKMQQFENQAEDNLKALQLKENQIEVQIKDPESKLNNFGGQLKELELTEKQYDEEKEFDTSNMDDQFSITIDGTSEEFGILDNLRKSSDPAKLVLDIILNPTIPLPKKGDKAVIIDESRIYLLEKLMIISPDIKSCVRDEALKLARELKANMKENTENSLEVLGFLMILSIYGLLTYFDEDEVFKLFASVASVAEYKIAVKLFRTLGFVHKVSGMLI