AlfalfaGEDB | MS.gene87013

M. sativa cultivar XinJiangDaYe / MS.gene87013

KEGG: -
GO: GO:0003674 GO:0004857 GO:0004866 GO:0030234 GO:0030414 GO:0061134 GO:0061135 GO:0098772
PFAM: PF00197.21

Nr:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene87013.t1	XP_013464493.1	82	205	31	1	1	205	1	199	5.10E-88	334

Swissprot:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene87013.t1	P29421	29.7	165	112	1	11	171	6	170	6.6e-12	72.4

Trembl:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene87013.t1	G8A259	82.0	205	31	1	1	205	1	199	3.6e-88	334.0

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network:

Gene1	Gene2	correlation coefficient	p_value	FDR
MS.gene052187	MS.gene87013	0.80807	3.71E-50	-1.69E-46
MS.gene053074	MS.gene87013	0.803004	4.29E-49	-1.69E-46
MS.gene058168	MS.gene87013	0.806069	9.84E-50	-1.69E-46

PPI:

Gene1	Gene2	Type

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene87013.t1	MTR_2g072980	81.951	205	31	1	1	205	1	199	6.81e-118	333
MS.gene87013.t1	MTR_2g073010	65.714	210	55	4	1	205	1	198	1.68e-88	259
MS.gene87013.t1	MTR_8g059790	47.291	203	83	6	1	188	1	194	2.94e-52	167
MS.gene87013.t1	MTR_0211s0080	45.960	198	87	6	1	188	1	188	3.42e-48	156
MS.gene87013.t1	MTR_0100s0150	44.776	201	88	6	1	188	1	191	1.18e-47	155
MS.gene87013.t1	MTR_8g461050	43.069	202	91	6	1	188	1	192	8.45e-46	150
MS.gene87013.t1	MTR_0302s0030	43.069	202	91	6	1	188	1	192	1.08e-45	150
MS.gene87013.t1	MTR_3g014820	42.365	203	92	6	1	188	1	193	2.46e-45	149
MS.gene87013.t1	MTR_3g014870	42.365	203	92	6	1	188	1	193	9.40e-44	145
MS.gene87013.t1	MTR_0084s0030	43.069	202	91	6	1	188	1	192	5.34e-43	143
MS.gene87013.t1	MTR_7g108815	43.069	202	89	8	1	188	1	190	1.59e-41	139
MS.gene87013.t1	MTR_8g060550	40.686	204	89	8	15	204	14	199	3.62e-41	138
MS.gene87013.t1	MTR_8g461690	40.686	204	89	8	15	204	14	199	3.62e-41	138
MS.gene87013.t1	MTR_6g078070	31.515	165	105	5	14	173	10	171	1.07e-15	72.8
MS.gene87013.t1	MTR_6g078100	29.756	205	122	8	14	205	10	205	7.30e-14	67.8
MS.gene87013.t1	MTR_6g078290	29.327	208	110	9	8	186	1	200	2.67e-11	60.8

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene87013.t1	AT1G17860	29.187	209	120	8	11	205	1	195	2.87e-14	68.6

Find 50 sgRNAs with CRISPR-Local

Find 62 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AGATCAGATGCACTCGATTT+TGG	0.197819	2.1:+25484982	MS.gene87013:CDS
ACAATCTACGGAATGGAAAT+TGG	0.201806	2.1:+25485179	MS.gene87013:CDS
GTTGGTAATTTGCATGAAAA+TGG	0.244897	2.1:+25485354	MS.gene87013:CDS
GTTTCTTGACCCACATATAA+AGG	0.297981	2.1:-25485042	None:intergenic
CGGAATGGAAATTGGGTGAT+AGG	0.325123	2.1:+25485187	MS.gene87013:CDS
ATACAGTTGTTAAGGTGAAT+AGG	0.332362	2.1:+25485118	MS.gene87013:CDS
CAAGCCTTAGCCTTATGTTA+TGG	0.345426	2.1:+25484863	MS.gene87013:CDS
GGTCAAGAAACTACAGATAT+TGG	0.348677	2.1:+25485054	MS.gene87013:CDS
TACAGTTGTTAAGGTGAATA+GGG	0.357658	2.1:+25485119	MS.gene87013:CDS
AGGTTGTGCTAATGTTGATA+TGG	0.401373	2.1:-25484896	None:intergenic
AAGACTCCACTTGGAATTGA+AGG	0.410273	2.1:+25485273	MS.gene87013:CDS
AAGGTTAATGTTCTCAAGGT+TGG	0.412184	2.1:+25485417	MS.gene87013:CDS
AGGTTAATGTTCTCAAGGTT+GGG	0.412594	2.1:+25485418	MS.gene87013:CDS
AACAGAAGATACAGTTGTTA+AGG	0.418077	2.1:+25485110	MS.gene87013:CDS
CGGTTCATGCCCTTTATATG+TGG	0.431349	2.1:+25485032	MS.gene87013:CDS
TTTCTTGACCCACATATAAA+GGG	0.438692	2.1:-25485041	None:intergenic
CAATCTACGGAATGGAAATT+GGG	0.450660	2.1:+25485180	MS.gene87013:CDS
TTCAAGTTCTGGACAATCTA+CGG	0.461337	2.1:+25485167	MS.gene87013:CDS
AATTGGGTGATAGGGACAAT+AGG	0.464287	2.1:+25485196	MS.gene87013:CDS
TTTGATCAATCTTGTTGTCA+TGG	0.481185	2.1:-25484836	None:intergenic
TCATGAAAAGCCATAACATA+AGG	0.507153	2.1:-25484873	None:intergenic
ACGGTTAATTGCGGTACGGT+TGG	0.512688	2.1:+25485336	MS.gene87013:CDS
ATAGGGACAATAGGAGTGGA+AGG	0.516567	2.1:+25485205	MS.gene87013:CDS
GTCACTTTGATAAATAGAAA+CGG	0.522150	2.1:+25485012	MS.gene87013:CDS
GGAATGGAAATTGGGTGATA+GGG	0.534497	2.1:+25485188	MS.gene87013:CDS
GGAAGGAGGCTTATTACCGC+GGG	0.539145	2.1:+25485222	MS.gene87013:CDS
TGGTATTAATAATGTTCTCA+AGG	0.548464	2.1:+25485398	MS.gene87013:CDS
TCTCAAGGTTAATGTTCTCA+AGG	0.553729	2.1:+25485413	MS.gene87013:CDS
CTGATCTTATGTAATACTGT+TGG	0.563143	2.1:-25484966	None:intergenic
CTTATTACCGCGGGGAGTGA+TGG	0.568025	2.1:+25485231	MS.gene87013:CDS
ACTGTATCTTCTGTTGCCAT+AGG	0.571927	2.1:-25485102	None:intergenic
GTTCTGGACAATCTACGGAA+TGG	0.572639	2.1:+25485172	MS.gene87013:CDS
GGTTCATGCCCTTTATATGT+GGG	0.576927	2.1:+25485033	MS.gene87013:CDS
ACCGCAATTAACCGTTTCCG+AGG	0.595491	2.1:-25485328	None:intergenic
AAAGCCATAACATAAGGCTA+AGG	0.601835	2.1:-25484867	None:intergenic
TACTGTTGGCCACTCTCAAG+AGG	0.608309	2.1:-25484952	None:intergenic
GGTGATAGGGACAATAGGAG+TGG	0.613024	2.1:+25485201	MS.gene87013:CDS
TTCTATTTATCAAAGTGACA+CGG	0.619288	2.1:-25485008	None:intergenic
CCGCAATTAACCGTTTCCGA+GGG	0.637522	2.1:-25485327	None:intergenic
AAAACACCTTCAATTCCAAG+TGG	0.642111	2.1:-25485279	None:intergenic
ACACGCCGTCCTCTTGAGAG+TGG	0.654084	2.1:+25484943	MS.gene87013:CDS
CCCTCGGAAACGGTTAATTG+CGG	0.663767	2.1:+25485327	MS.gene87013:CDS
GAAACTACAGATATTGGACA+AGG	0.666365	2.1:+25485060	MS.gene87013:CDS
GGAAACGGTTAATTGCGGTA+CGG	0.670786	2.1:+25485332	MS.gene87013:CDS
TGGAAGGAGGCTTATTACCG+CGG	0.680004	2.1:+25485221	MS.gene87013:CDS
GGGACAATAGGAGTGGAAGG+AGG	0.693684	2.1:+25485208	MS.gene87013:CDS
GTTGGCCACTCTCAAGAGGA+CGG	0.704210	2.1:-25484948	None:intergenic
AAACTACAGATATTGGACAA+GGG	0.717751	2.1:+25485061	MS.gene87013:CDS
GAAGGAGGCTTATTACCGCG+GGG	0.754640	2.1:+25485223	MS.gene87013:CDS
ATAACTACCATCACTCCCCG+CGG	0.756310	2.1:-25485238	None:intergenic

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!!!	TTTTAGGTAATTATAATATT+AGG	+	chr2.1:25485298-25485317	MS.gene87013:CDS	10.0%
!!!	TGAAAATGGTAAGATTTTAT+TGG	+	chr2.1:25485368-25485387	MS.gene87013:CDS	20.0%
!	TGGTATTAATAATGTTCTCA+AGG	+	chr2.1:25485398-25485417	MS.gene87013:CDS	25.0%
!	TTCTATTTATCAAAGTGACA+CGG	-	chr2.1:25485011-25485030	None:intergenic	25.0%
!!	GTCACTTTGATAAATAGAAA+CGG	+	chr2.1:25485012-25485031	MS.gene87013:CDS	25.0%
!!!	ATCTGTTATTTTTACTCCTA+TGG	+	chr2.1:25485086-25485105	MS.gene87013:CDS	25.0%
	AAACTACAGATATTGGACAA+GGG	+	chr2.1:25485061-25485080	MS.gene87013:CDS	30.0%
	AACAGAAGATACAGTTGTTA+AGG	+	chr2.1:25485110-25485129	MS.gene87013:CDS	30.0%
	ATACAGTTGTTAAGGTGAAT+AGG	+	chr2.1:25485118-25485137	MS.gene87013:CDS	30.0%
	CTGATCTTATGTAATACTGT+TGG	-	chr2.1:25484969-25484988	None:intergenic	30.0%
	TACAGTTGTTAAGGTGAATA+GGG	+	chr2.1:25485119-25485138	MS.gene87013:CDS	30.0%
	TCATGAAAAGCCATAACATA+AGG	-	chr2.1:25484876-25484895	None:intergenic	30.0%
	TTTCTTGACCCACATATAAA+GGG	-	chr2.1:25485044-25485063	None:intergenic	30.0%
!	TAAGGTGAATAGGGATTTTA+AGG	+	chr2.1:25485128-25485147	MS.gene87013:CDS	30.0%
!	TCAAGAACTTCTTCATTTTC+AGG	-	chr2.1:25484919-25484938	None:intergenic	30.0%
!!	GTTGGTAATTTGCATGAAAA+TGG	+	chr2.1:25485354-25485373	MS.gene87013:CDS	30.0%
!!	TAATATTAGGTTTTGTCCCT+CGG	+	chr2.1:25485311-25485330	MS.gene87013:CDS	30.0%
!!!	AAGATTTTATTGGCTTTGGA+TGG	+	chr2.1:25485378-25485397	MS.gene87013:CDS	30.0%
!!!	TGGTAAGATTTTATTGGCTT+TGG	+	chr2.1:25485374-25485393	MS.gene87013:CDS	30.0%
	AAAACACCTTCAATTCCAAG+TGG	-	chr2.1:25485282-25485301	None:intergenic	35.0%
	AAAGCCATAACATAAGGCTA+AGG	-	chr2.1:25484870-25484889	None:intergenic	35.0%
	AAGGTTAATGTTCTCAAGGT+TGG	+	chr2.1:25485417-25485436	MS.gene87013:CDS	35.0%
	ACAATCTACGGAATGGAAAT+TGG	+	chr2.1:25485179-25485198	MS.gene87013:CDS	35.0%
	AGGTTAATGTTCTCAAGGTT+GGG	+	chr2.1:25485418-25485437	MS.gene87013:CDS	35.0%
	AGGTTGTGCTAATGTTGATA+TGG	-	chr2.1:25484899-25484918	None:intergenic	35.0%
	CAATCTACGGAATGGAAATT+GGG	+	chr2.1:25485180-25485199	MS.gene87013:CDS	35.0%
	GAAACTACAGATATTGGACA+AGG	+	chr2.1:25485060-25485079	MS.gene87013:CDS	35.0%
	GGTCAAGAAACTACAGATAT+TGG	+	chr2.1:25485054-25485073	MS.gene87013:CDS	35.0%
	GTTTCTTGACCCACATATAA+AGG	-	chr2.1:25485045-25485064	None:intergenic	35.0%
	TCTCAAGGTTAATGTTCTCA+AGG	+	chr2.1:25485413-25485432	MS.gene87013:CDS	35.0%
!!	AGAATTTTGAAGACTCCACT+TGG	+	chr2.1:25485264-25485283	MS.gene87013:CDS	35.0%
!!	TTCAAGTTCTGGACAATCTA+CGG	+	chr2.1:25485167-25485186	MS.gene87013:CDS	35.0%
!!	TTTTCAGCTTCTTCAAGTTC+TGG	+	chr2.1:25485156-25485175	MS.gene87013:CDS	35.0%
!!!	CTTGGAATTGAAGGTGTTTT+AGG	+	chr2.1:25485282-25485301	MS.gene87013:CDS	35.0%
	AAGACTCCACTTGGAATTGA+AGG	+	chr2.1:25485273-25485292	MS.gene87013:CDS	40.0%
	AATTGGGTGATAGGGACAAT+AGG	+	chr2.1:25485196-25485215	MS.gene87013:CDS	40.0%
	ACTGTATCTTCTGTTGCCAT+AGG	-	chr2.1:25485105-25485124	None:intergenic	40.0%
	GGAATGGAAATTGGGTGATA+GGG	+	chr2.1:25485188-25485207	MS.gene87013:CDS	40.0%
	GGTTCATGCCCTTTATATGT+GGG	+	chr2.1:25485033-25485052	MS.gene87013:CDS	40.0%
!	AGATCAGATGCACTCGATTT+TGG	+	chr2.1:25484982-25485001	MS.gene87013:CDS	40.0%
!!	CAAGCCTTAGCCTTATGTTA+TGG	+	chr2.1:25484863-25484882	MS.gene87013:CDS	40.0%
	ATAGGGACAATAGGAGTGGA+AGG	+	chr2.1:25485205-25485224	MS.gene87013:CDS	45.0%
	CGGAATGGAAATTGGGTGAT+AGG	+	chr2.1:25485187-25485206	MS.gene87013:CDS	45.0%
	CGGTTCATGCCCTTTATATG+TGG	+	chr2.1:25485032-25485051	MS.gene87013:CDS	45.0%
	GGAAACGGTTAATTGCGGTA+CGG	+	chr2.1:25485332-25485351	MS.gene87013:CDS	45.0%
!	GTTCTGGACAATCTACGGAA+TGG	+	chr2.1:25485172-25485191	MS.gene87013:CDS	45.0%
!	TCAGATGCACTCGATTTTGG+AGG	+	chr2.1:25484985-25485004	MS.gene87013:CDS	45.0%
!!	TAGGTTTTGTCCCTCGGAAA+CGG	+	chr2.1:25485317-25485336	MS.gene87013:CDS	45.0%
	ACCGCAATTAACCGTTTCCG+AGG	-	chr2.1:25485331-25485350	None:intergenic	50.0%
	ACGGTTAATTGCGGTACGGT+TGG	+	chr2.1:25485336-25485355	MS.gene87013:CDS	50.0%
	ATAACTACCATCACTCCCCG+CGG	-	chr2.1:25485241-25485260	None:intergenic	50.0%
	CCCTCGGAAACGGTTAATTG+CGG	+	chr2.1:25485327-25485346	MS.gene87013:CDS	50.0%
	CCGCAATTAACCGTTTCCGA+GGG	-	chr2.1:25485330-25485349	None:intergenic	50.0%
	GGTGATAGGGACAATAGGAG+TGG	+	chr2.1:25485201-25485220	MS.gene87013:CDS	50.0%
	TACTGTTGGCCACTCTCAAG+AGG	-	chr2.1:25484955-25484974	None:intergenic	50.0%
	TGGAAGGAGGCTTATTACCG+CGG	+	chr2.1:25485221-25485240	MS.gene87013:CDS	50.0%
	CTTATTACCGCGGGGAGTGA+TGG	+	chr2.1:25485231-25485250	MS.gene87013:CDS	55.0%
	GAAGGAGGCTTATTACCGCG+GGG	+	chr2.1:25485223-25485242	MS.gene87013:CDS	55.0%
	GGAAGGAGGCTTATTACCGC+GGG	+	chr2.1:25485222-25485241	MS.gene87013:CDS	55.0%
	GGGACAATAGGAGTGGAAGG+AGG	+	chr2.1:25485208-25485227	MS.gene87013:CDS	55.0%
	GTTGGCCACTCTCAAGAGGA+CGG	-	chr2.1:25484951-25484970	None:intergenic	55.0%
	ACACGCCGTCCTCTTGAGAG+TGG	+	chr2.1:25484943-25484962	MS.gene87013:CDS	60.0%

Chromosome	Type	Strat	End	Strand	Name
chr2.1	gene	25484838	25485455	25484838	ID=MS.gene87013
chr2.1	mRNA	25484838	25485455	25484838	ID=MS.gene87013.t1;Parent=MS.gene87013
chr2.1	exon	25484838	25485455	25484838	ID=MS.gene87013.t1.exon1;Parent=MS.gene87013.t1
chr2.1	CDS	25484838	25485455	25484838	ID=cds.MS.gene87013.t1;Parent=MS.gene87013.t1

Gene Sequence

>MS.gene87013

ATGACAACAAGATTGATCAAAATCACAAGCCTTAGCCTTATGTTATGGCTTTTCATGACCATATCAACATTAGCACAACCTGAAAATGAAGAAGTTCTTGACTTAACACGCCGTCCTCTTGAGAGTGGCCAACAGTATTACATAAGATCAGATGCACTCGATTTTGGAGGCCGTGTCACTTTGATAAATAGAAACGGTTCATGCCCTTTATATGTGGGTCAAGAAACTACAGATATTGGACAAGGGTTATCTGTTATTTTTACTCCTATGGCAACAGAAGATACAGTTGTTAAGGTGAATAGGGATTTTAAGGTGAAATTTTCAGCTTCTTCAAGTTCTGGACAATCTACGGAATGGAAATTGGGTGATAGGGACAATAGGAGTGGAAGGAGGCTTATTACCGCGGGGAGTGATGGTAGTTATTTTAGAATTTTGAAGACTCCACTTGGAATTGAAGGTGTTTTAGGTAATTATAATATTAGGTTTTGTCCCTCGGAAACGGTTAATTGCGGTACGGTTGGTAATTTGCATGAAAATGGTAAGATTTTATTGGCTTTGGATGGTATTAATAATGTTCTCAAGGTTAATGTTCTCAAGGTTGGGTTCGAGAGAGCGTAA

Protein sequence

>MS.gene87013.t1

MTTRLIKITSLSLMLWLFMTISTLAQPENEEVLDLTRRPLESGQQYYIRSDALDFGGRVTLINRNGSCPLYVGQETTDIGQGLSVIFTPMATEDTVVKVNRDFKVKFSASSSSGQSTEWKLGDRDNRSGRRLITAGSDGSYFRILKTPLGIEGVLGNYNIRFCPSETVNCGTVGNLHENGKILLALDGINNVLKVNVLKVGFERA