Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene87617.t1 | RHN79930.1 | 82.4 | 119 | 21 | 0 | 1 | 119 | 39 | 157 | 1.30E-48 | 202.2 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene87617.t1 | A0A072VLY4 | 82.4 | 119 | 21 | 0 | 1 | 119 | 235 | 353 | 9.6e-49 | 202.2 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene049536 | MS.gene87617 | 0.808111 | 3.64E-50 | -1.69E-46 |
| MS.gene053356 | MS.gene87617 | 0.800208 | 1.61E-48 | -1.69E-46 |
| MS.gene059009 | MS.gene87617 | 0.88334 | 4.87E-71 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 0 sgRNAs with CRISPR-Local
Find 31 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| ! | AAATGAAAGAAGGAAAATGT+GGG | + | 11362:19181-19200 | MS.gene87617:CDS | 25.0% |
| ! | TAATTGGATAGAAGAAACAA+GGG | + | 11362:19238-19257 | MS.gene87617:CDS | 25.0% |
| ! | TCTTGATTAGAAGATTGATT+TGG | - | 11362:19155-19174 | None:intergenic | 25.0% |
| AAGAACATCCAAATGAAAGA+AGG | + | 11362:19171-19190 | MS.gene87617:CDS | 30.0% | |
| AAGAGAGGAAAAGAAAGAAA+TGG | - | 11362:19459-19478 | None:intergenic | 30.0% | |
| AATTGGATAGAAGAAACAAG+GGG | + | 11362:19239-19258 | MS.gene87617:CDS | 30.0% | |
| ATCTACAATTCCAAGGTAAT+TGG | + | 11362:19222-19241 | MS.gene87617:CDS | 30.0% | |
| ATGAACTTTACGAATTCATG+TGG | - | 11362:19425-19444 | None:intergenic | 30.0% | |
| CAAATGAAAGAAGGAAAATG+TGG | + | 11362:19180-19199 | MS.gene87617:CDS | 30.0% | |
| CAAGAAAATACAAATACTGG+AGG | + | 11362:19338-19357 | MS.gene87617:CDS | 30.0% | |
| GTAATTGGATAGAAGAAACA+AGG | + | 11362:19237-19256 | MS.gene87617:CDS | 30.0% | |
| TGGCAAGAAAATACAAATAC+TGG | + | 11362:19335-19354 | MS.gene87617:CDS | 30.0% | |
| TTTCTTCTATCCAATTACCT+TGG | - | 11362:19235-19254 | None:intergenic | 30.0% | |
| ! | ATTTCTTTCTTTTCCTCTCT+TGG | + | 11362:19458-19477 | MS.gene87617:CDS | 30.0% |
| ! | CACATTTTCCTTCTTTCATT+TGG | - | 11362:19182-19201 | None:intergenic | 30.0% |
| ! | GGAAAATGTGGGAAAAATTT+TGG | + | 11362:19192-19211 | MS.gene87617:CDS | 30.0% |
| ! | TGTTGATTGAAAAGTCATTG+TGG | - | 11362:19294-19313 | None:intergenic | 30.0% |
| !! | TTGATTTGGTTGTTCTTGAT+TGG | - | 11362:19141-19160 | None:intergenic | 30.0% |
| ACCTTGGAATTGTAGATACT+TGG | - | 11362:19219-19238 | None:intergenic | 35.0% | |
| TCCAAGTATCTACAATTCCA+AGG | + | 11362:19215-19234 | MS.gene87617:CDS | 35.0% | |
| ACTTATGGTGTATGTGAAGC+AGG | + | 11362:19374-19393 | MS.gene87617:CDS | 40.0% | |
| AGTCATTGTGGCAATTACAG+TGG | - | 11362:19282-19301 | None:intergenic | 40.0% | |
| GGACTCAATTGCACTACTTA+TGG | + | 11362:19359-19378 | MS.gene87617:CDS | 40.0% | |
| CAATCAACATTAAGTCCGCC+AGG | + | 11362:19305-19324 | MS.gene87617:CDS | 45.0% | |
| CTTTACGAATTCATGTGGCG+AGG | - | 11362:19420-19439 | None:intergenic | 45.0% | |
| ! | TTTTCTTGCCAAACACCACC+TGG | - | 11362:19326-19345 | None:intergenic | 45.0% |
| !! | AACAAGGGGAGCATTGATGT+TGG | + | 11362:19253-19272 | MS.gene87617:CDS | 45.0% |
| TCAACATTAAGTCCGCCAGG+TGG | + | 11362:19308-19327 | MS.gene87617:CDS | 50.0% | |
| !! | AAGGGGAGCATTGATGTTGG+TGG | + | 11362:19256-19275 | MS.gene87617:CDS | 50.0% |
| TAAGTCCGCCAGGTGGTGTT+TGG | + | 11362:19315-19334 | MS.gene87617:CDS | 55.0% | |
| TCTTGCCAAACACCACCTGG+CGG | - | 11362:19323-19342 | None:intergenic | 55.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| 11362 | gene | 19123 | 19481 | 19123 | ID=MS.gene87617 |
| 11362 | mRNA | 19123 | 19481 | 19123 | ID=MS.gene87617.t1;Parent=MS.gene87617 |
| 11362 | exon | 19123 | 19481 | 19123 | ID=MS.gene87617.t1.exon1;Parent=MS.gene87617.t1 |
| 11362 | CDS | 19123 | 19481 | 19123 | ID=cds.MS.gene87617.t1;Parent=MS.gene87617.t1 |
| Gene Sequence |
| Protein sequence |
