Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene88664.t1 | XP_003610152.2 | 98.2 | 275 | 5 | 0 | 1 | 275 | 1 | 275 | 6.20E-158 | 566.6 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene88664.t1 | Q38910 | 70.4 | 284 | 66 | 2 | 8 | 275 | 5 | 286 | 1.0e-116 | 421.0 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene88664.t1 | G7JD11 | 98.2 | 275 | 5 | 0 | 1 | 275 | 1 | 275 | 4.5e-158 | 566.6 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene052083 | MS.gene88664 | 0.82465 | 7.20E-54 | -1.69E-46 |
| MS.gene055661 | MS.gene88664 | 0.800427 | 1.45E-48 | -1.69E-46 |
| MS.gene055977 | MS.gene88664 | 0.850079 | 2.14E-60 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene88664.t1 | MTR_4g128570 | 98.182 | 275 | 5 | 0 | 1 | 275 | 1 | 275 | 0.0 | 568 |
| MS.gene88664.t1 | MTR_7g093530 | 79.705 | 271 | 41 | 1 | 16 | 272 | 9 | 279 | 5.41e-164 | 456 |
| MS.gene88664.t1 | MTR_4g128410 | 78.967 | 271 | 43 | 1 | 16 | 272 | 10 | 280 | 1.77e-161 | 449 |
| MS.gene88664.t1 | MTR_4g128580 | 80.899 | 267 | 33 | 1 | 27 | 275 | 22 | 288 | 6.31e-158 | 441 |
| MS.gene88664.t1 | MTR_4g128400 | 68.198 | 283 | 75 | 2 | 6 | 274 | 4 | 285 | 2.76e-145 | 409 |
| MS.gene88664.t1 | MTR_4g128590 | 69.580 | 286 | 51 | 3 | 10 | 275 | 9 | 278 | 3.01e-143 | 403 |
| MS.gene88664.t1 | MTR_4g126920 | 71.536 | 267 | 61 | 1 | 22 | 273 | 16 | 282 | 2.32e-142 | 401 |
| MS.gene88664.t1 | MTR_2g038860 | 68.327 | 281 | 70 | 1 | 14 | 275 | 9 | 289 | 6.95e-142 | 400 |
| MS.gene88664.t1 | MTR_2g038705 | 68.327 | 281 | 70 | 1 | 14 | 275 | 9 | 289 | 6.95e-142 | 400 |
| MS.gene88664.t1 | MTR_8g081040 | 67.384 | 279 | 80 | 2 | 4 | 273 | 6 | 282 | 2.36e-138 | 391 |
| MS.gene88664.t1 | MTR_3g084620 | 67.857 | 280 | 79 | 3 | 1 | 272 | 1 | 277 | 2.49e-138 | 391 |
| MS.gene88664.t1 | MTR_3g084630 | 69.517 | 269 | 73 | 2 | 12 | 272 | 10 | 277 | 1.86e-137 | 389 |
| MS.gene88664.t1 | MTR_8g102220 | 64.835 | 273 | 81 | 4 | 6 | 275 | 9 | 269 | 3.76e-126 | 360 |
| MS.gene88664.t1 | MTR_5g091730 | 51.481 | 270 | 122 | 1 | 12 | 272 | 9 | 278 | 2.56e-100 | 295 |
| MS.gene88664.t1 | MTR_8g102910 | 51.957 | 281 | 113 | 4 | 12 | 272 | 1 | 279 | 7.29e-100 | 293 |
| MS.gene88664.t1 | MTR_6g088320 | 51.971 | 279 | 113 | 4 | 13 | 272 | 7 | 283 | 3.71e-98 | 289 |
| MS.gene88664.t1 | MTR_3g089540 | 52.308 | 260 | 113 | 1 | 24 | 272 | 23 | 282 | 9.70e-98 | 288 |
| MS.gene88664.t1 | MTR_6g033085 | 51.568 | 287 | 120 | 4 | 5 | 273 | 3 | 288 | 1.08e-96 | 286 |
| MS.gene88664.t1 | MTR_6g033055 | 51.748 | 286 | 116 | 5 | 6 | 273 | 7 | 288 | 4.09e-96 | 284 |
| MS.gene88664.t1 | MTR_7g084760 | 51.246 | 281 | 120 | 4 | 7 | 272 | 9 | 287 | 6.24e-95 | 281 |
| MS.gene88664.t1 | MTR_7g084770 | 54.086 | 257 | 102 | 3 | 31 | 272 | 32 | 287 | 1.41e-94 | 280 |
| MS.gene88664.t1 | MTR_7g084750 | 51.799 | 278 | 116 | 5 | 12 | 272 | 11 | 287 | 1.63e-93 | 278 |
| MS.gene88664.t1 | MTR_2g089140 | 47.735 | 287 | 124 | 5 | 10 | 273 | 4 | 287 | 1.47e-92 | 275 |
| MS.gene88664.t1 | MTR_4g097640 | 47.653 | 277 | 128 | 5 | 12 | 272 | 12 | 287 | 2.09e-90 | 270 |
| MS.gene88664.t1 | MTR_4g097670 | 47.653 | 277 | 128 | 5 | 12 | 272 | 12 | 287 | 2.09e-90 | 270 |
| MS.gene88664.t1 | MTR_4g097700 | 47.653 | 277 | 125 | 5 | 15 | 272 | 49 | 324 | 8.58e-90 | 270 |
| MS.gene88664.t1 | MTR_1g105900 | 47.482 | 278 | 130 | 2 | 12 | 273 | 15 | 292 | 1.06e-89 | 268 |
| MS.gene88664.t1 | MTR_4g045853 | 46.897 | 290 | 125 | 6 | 7 | 273 | 6 | 289 | 1.91e-89 | 268 |
| MS.gene88664.t1 | MTR_1g105495 | 45.993 | 287 | 127 | 5 | 16 | 275 | 12 | 297 | 1.25e-83 | 253 |
| MS.gene88664.t1 | MTR_2g089140 | 51.111 | 225 | 86 | 4 | 72 | 273 | 1 | 224 | 9.06e-80 | 240 |
| MS.gene88664.t1 | MTR_8g013055 | 46.304 | 257 | 121 | 4 | 31 | 272 | 31 | 285 | 9.32e-79 | 240 |
| MS.gene88664.t1 | MTR_7g056090 | 43.985 | 266 | 128 | 7 | 12 | 260 | 12 | 273 | 4.34e-68 | 213 |
| MS.gene88664.t1 | MTR_7g102260 | 40.000 | 270 | 137 | 7 | 24 | 274 | 30 | 293 | 1.73e-60 | 193 |
| MS.gene88664.t1 | MTR_2g049030 | 39.350 | 277 | 142 | 9 | 13 | 269 | 10 | 280 | 4.70e-58 | 187 |
| MS.gene88664.t1 | MTR_1g063020 | 40.840 | 262 | 130 | 9 | 30 | 272 | 39 | 294 | 3.60e-57 | 185 |
| MS.gene88664.t1 | MTR_4g057450 | 39.465 | 299 | 144 | 10 | 5 | 272 | 12 | 304 | 4.09e-57 | 185 |
| MS.gene88664.t1 | MTR_2g049130 | 40.684 | 263 | 125 | 9 | 31 | 274 | 29 | 279 | 2.51e-56 | 182 |
| MS.gene88664.t1 | MTR_2g095800 | 41.667 | 264 | 126 | 10 | 30 | 272 | 53 | 309 | 3.40e-55 | 180 |
| MS.gene88664.t1 | MTR_1g033420 | 34.364 | 291 | 162 | 6 | 10 | 273 | 4 | 292 | 1.05e-51 | 172 |
| MS.gene88664.t1 | MTR_5g029100 | 34.884 | 258 | 142 | 7 | 39 | 272 | 50 | 305 | 2.03e-47 | 161 |
| MS.gene88664.t1 | MTR_8g064180 | 34.981 | 263 | 144 | 6 | 35 | 272 | 50 | 310 | 4.55e-47 | 159 |
| MS.gene88664.t1 | MTR_8g090100 | 32.830 | 265 | 153 | 7 | 31 | 273 | 21 | 282 | 1.54e-43 | 150 |
| MS.gene88664.t1 | MTR_5g029100 | 35.043 | 234 | 126 | 7 | 63 | 272 | 43 | 274 | 6.48e-43 | 149 |
| MS.gene88664.t1 | MTR_8g479340 | 40.404 | 99 | 42 | 3 | 193 | 275 | 41 | 138 | 2.78e-16 | 74.3 |
| MS.gene88664.t1 | MTR_8g032640 | 31.609 | 174 | 111 | 4 | 53 | 224 | 33 | 200 | 2.45e-14 | 70.5 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene88664.t1 | AT4G25810 | 70.819 | 281 | 64 | 2 | 11 | 275 | 8 | 286 | 5.87e-151 | 423 |
| MS.gene88664.t1 | AT5G57560 | 68.662 | 284 | 71 | 2 | 9 | 275 | 2 | 284 | 4.17e-148 | 416 |
| MS.gene88664.t1 | AT4G30270 | 71.103 | 263 | 74 | 1 | 13 | 275 | 8 | 268 | 2.84e-146 | 410 |
| MS.gene88664.t1 | AT5G57550 | 68.345 | 278 | 72 | 2 | 11 | 272 | 5 | 282 | 3.58e-144 | 406 |
| MS.gene88664.t1 | AT5G57530 | 69.231 | 260 | 66 | 1 | 27 | 272 | 23 | 282 | 1.80e-134 | 381 |
| MS.gene88664.t1 | AT5G57540 | 68.726 | 259 | 67 | 1 | 28 | 272 | 23 | 281 | 1.75e-133 | 379 |
| MS.gene88664.t1 | AT4G25820 | 66.792 | 265 | 74 | 1 | 22 | 272 | 21 | 285 | 9.19e-133 | 377 |
| MS.gene88664.t1 | AT5G48070 | 59.712 | 278 | 101 | 2 | 7 | 273 | 5 | 282 | 1.89e-129 | 369 |
| MS.gene88664.t1 | AT3G23730 | 63.604 | 283 | 84 | 3 | 11 | 275 | 8 | 289 | 2.56e-129 | 369 |
| MS.gene88664.t1 | AT4G14130 | 61.837 | 283 | 92 | 2 | 8 | 275 | 6 | 287 | 2.90e-129 | 369 |
| MS.gene88664.t1 | AT2G18800 | 59.524 | 294 | 92 | 3 | 8 | 274 | 5 | 298 | 7.75e-129 | 368 |
| MS.gene88664.t1 | AT4G30290 | 62.500 | 272 | 91 | 2 | 13 | 273 | 6 | 277 | 2.39e-123 | 353 |
| MS.gene88664.t1 | AT4G30280 | 59.857 | 279 | 101 | 2 | 6 | 273 | 4 | 282 | 6.70e-122 | 349 |
| MS.gene88664.t1 | AT1G65310 | 63.424 | 257 | 83 | 2 | 28 | 273 | 48 | 304 | 1.38e-119 | 344 |
| MS.gene88664.t1 | AT1G65310 | 63.424 | 257 | 83 | 2 | 28 | 273 | 26 | 282 | 1.62e-119 | 343 |
| MS.gene88664.t1 | AT4G28850 | 48.973 | 292 | 126 | 4 | 1 | 273 | 1 | 288 | 3.52e-97 | 287 |
| MS.gene88664.t1 | AT5G13870 | 52.128 | 282 | 114 | 4 | 11 | 272 | 7 | 287 | 1.52e-95 | 283 |
| MS.gene88664.t1 | AT5G13870 | 52.128 | 282 | 114 | 4 | 11 | 272 | 7 | 287 | 1.52e-95 | 283 |
| MS.gene88664.t1 | AT5G13870 | 52.128 | 282 | 114 | 4 | 11 | 272 | 7 | 287 | 1.52e-95 | 283 |
| MS.gene88664.t1 | AT5G65730 | 46.479 | 284 | 131 | 6 | 7 | 274 | 14 | 292 | 9.32e-93 | 276 |
| MS.gene88664.t1 | AT2G06850 | 53.307 | 257 | 104 | 3 | 31 | 272 | 35 | 290 | 5.67e-91 | 271 |
| MS.gene88664.t1 | AT4G37800 | 48.201 | 278 | 126 | 5 | 14 | 274 | 15 | 291 | 1.17e-89 | 268 |
| MS.gene88664.t1 | AT4G03210 | 45.520 | 279 | 132 | 4 | 11 | 272 | 9 | 284 | 1.43e-87 | 263 |
| MS.gene88664.t1 | AT1G11545 | 45.918 | 294 | 134 | 6 | 6 | 275 | 10 | 302 | 7.69e-86 | 259 |
| MS.gene88664.t1 | AT2G14620 | 46.071 | 280 | 133 | 4 | 11 | 273 | 17 | 295 | 8.72e-86 | 258 |
| MS.gene88664.t1 | AT2G06850 | 55.507 | 227 | 85 | 3 | 61 | 272 | 5 | 230 | 1.28e-85 | 256 |
| MS.gene88664.t1 | AT4G03210 | 51.477 | 237 | 94 | 4 | 53 | 272 | 24 | 256 | 3.01e-83 | 251 |
| MS.gene88664.t1 | AT4G13090 | 44.484 | 281 | 137 | 5 | 9 | 272 | 10 | 288 | 2.26e-79 | 242 |
| MS.gene88664.t1 | AT2G14620 | 50.218 | 229 | 96 | 4 | 62 | 273 | 18 | 245 | 6.75e-76 | 231 |
| MS.gene88664.t1 | AT3G25050 | 45.174 | 259 | 121 | 5 | 31 | 272 | 35 | 289 | 1.98e-75 | 232 |
| MS.gene88664.t1 | AT4G13080 | 45.703 | 256 | 122 | 5 | 31 | 272 | 39 | 291 | 8.87e-75 | 230 |
| MS.gene88664.t1 | AT2G14620 | 50.909 | 220 | 90 | 4 | 71 | 273 | 16 | 234 | 6.22e-74 | 226 |
| MS.gene88664.t1 | AT2G36870 | 41.221 | 262 | 129 | 7 | 30 | 272 | 42 | 297 | 2.96e-58 | 188 |
| MS.gene88664.t1 | AT3G44990 | 41.270 | 252 | 124 | 7 | 31 | 263 | 39 | 285 | 3.64e-56 | 182 |
| MS.gene88664.t1 | AT1G32170 | 33.562 | 292 | 170 | 6 | 4 | 273 | 5 | 294 | 2.98e-52 | 174 |
| MS.gene88664.t1 | AT2G01850 | 32.993 | 294 | 166 | 6 | 10 | 274 | 1 | 292 | 2.74e-51 | 171 |
| MS.gene88664.t1 | AT1G14720 | 32.990 | 291 | 164 | 6 | 13 | 274 | 4 | 292 | 5.90e-51 | 170 |
| MS.gene88664.t1 | AT1G10550 | 35.769 | 260 | 136 | 6 | 42 | 272 | 52 | 309 | 6.37e-48 | 162 |
| MS.gene88664.t1 | AT3G48580 | 36.078 | 255 | 133 | 8 | 30 | 272 | 37 | 273 | 6.34e-45 | 153 |
| MS.gene88664.t1 | AT4G18990 | 32.463 | 268 | 146 | 6 | 39 | 273 | 48 | 313 | 1.50e-42 | 149 |
| MS.gene88664.t1 | AT3G48580 | 35.268 | 224 | 120 | 6 | 61 | 272 | 4 | 214 | 1.31e-39 | 137 |
| MS.gene88664.t1 | AT4G18990 | 33.000 | 200 | 102 | 5 | 104 | 273 | 42 | 239 | 1.20e-34 | 126 |
| MS.gene88664.t1 | AT2G36870 | 45.324 | 139 | 66 | 3 | 30 | 160 | 42 | 178 | 5.39e-33 | 119 |
| MS.gene88664.t1 | AT3G48580 | 30.980 | 255 | 126 | 9 | 30 | 272 | 37 | 253 | 5.79e-28 | 108 |
Find 61 sgRNAs with CRISPR-Local
Find 72 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| CACTGTCCTCTTATCTCTAT+TGG | 0.164509 | 8.1:-4420339 | MS.gene88664:CDS |
| ATCCTCATTGGTTGATTCTT+TGG | 0.185511 | 8.1:+4419542 | None:intergenic |
| ATGTTCTTGAATTCTCTTAT+TGG | 0.197118 | 8.1:+4419587 | None:intergenic |
| CTCGACAAAGCCTCTGGTTC+TGG | 0.236949 | 8.1:-4420200 | MS.gene88664:CDS |
| TTCACACCTACTCCATTCTT+TGG | 0.237903 | 8.1:-4419810 | MS.gene88664:CDS |
| TGAAATTGACTTTGAATTCT+TGG | 0.248522 | 8.1:-4419938 | MS.gene88664:CDS |
| TGTCATCAAAAGGATCAAAT+TGG | 0.258251 | 8.1:-4419963 | MS.gene88664:CDS |
| CTCCATTCACTGCATCTTAT+AGG | 0.269112 | 8.1:-4419448 | MS.gene88664:CDS |
| GAGAGCAACAATTCCATCTT+TGG | 0.288359 | 8.1:-4419852 | MS.gene88664:CDS |
| GAAATTGACTTTGAATTCTT+GGG | 0.327454 | 8.1:-4419937 | MS.gene88664:CDS |
| GCCAAAGAGATATTCATTCT+TGG | 0.330231 | 8.1:+4420169 | None:intergenic |
| TGCACTCCGTTGGAAGGCCT+TGG | 0.333111 | 8.1:+4419291 | None:intergenic |
| TGAGGATATACTCAAGTCTA+TGG | 0.355078 | 8.1:-4419526 | MS.gene88664:CDS |
| TCCAAGAATGAATATCTCTT+TGG | 0.372292 | 8.1:-4420170 | MS.gene88664:CDS |
| GCACTCCGTTGGAAGGCCTT+GGG | 0.382643 | 8.1:+4419292 | None:intergenic |
| AGAGATATTCATTCTTGGAT+TGG | 0.408705 | 8.1:+4420174 | None:intergenic |
| AGACTTGAGTATATCCTCAT+TGG | 0.432038 | 8.1:+4419530 | None:intergenic |
| GTCTATGGAATGCTGATGAT+TGG | 0.434923 | 8.1:-4419511 | MS.gene88664:CDS |
| TTGACCGTTGTCGAGTATCT+TGG | 0.435436 | 8.1:+4420238 | None:intergenic |
| GTCATCAAAAGGATCAAATT+GGG | 0.451206 | 8.1:-4419962 | MS.gene88664:CDS |
| CTTTCCCTCGACAAAGCCTC+TGG | 0.461846 | 8.1:-4420206 | MS.gene88664:CDS |
| ATGCAGCTCAAGCTTGTACC+TGG | 0.480284 | 8.1:-4420137 | MS.gene88664:CDS |
| GAATTCTTGGGGAATGTAAG+TGG | 0.491509 | 8.1:-4419925 | MS.gene88664:CDS |
| TTCAAGAACATGGAATCAAA+AGG | 0.493604 | 8.1:-4419575 | MS.gene88664:CDS |
| CAGTTGGATCAAACCAAAGA+TGG | 0.493971 | 8.1:+4419839 | None:intergenic |
| GGCATGCAGTTGTCATCAAA+AGG | 0.496860 | 8.1:-4419973 | MS.gene88664:intron |
| ACAGTGCCTGCAGAATTTCC+AGG | 0.499070 | 8.1:+4420119 | None:intergenic |
| TTCCTATAAGATGCAGTGAA+TGG | 0.501342 | 8.1:+4419446 | None:intergenic |
| GCGGTCTTGTCAAGACAGAT+TGG | 0.512024 | 8.1:-4419478 | MS.gene88664:CDS |
| TTCATTACAACATATGCAGC+AGG | 0.518113 | 8.1:-4420305 | MS.gene88664:CDS |
| CTTGTACCTGGAAATTCTGC+AGG | 0.520832 | 8.1:-4420125 | MS.gene88664:CDS |
| AATGTGTTTAGTCAAGGAAA+AGG | 0.525149 | 8.1:-4419880 | MS.gene88664:CDS |
| ATCAGAACTTTGATATAACA+TGG | 0.536100 | 8.1:-4420274 | MS.gene88664:CDS |
| TCTATGGAATGCTGATGATT+GGG | 0.538637 | 8.1:-4419510 | MS.gene88664:CDS |
| GCGTTGAGGATTCCAAAGAA+TGG | 0.542490 | 8.1:+4419798 | None:intergenic |
| CACACCAATGTGTTTAGTCA+AGG | 0.543031 | 8.1:-4419886 | MS.gene88664:CDS |
| AAATTGACTTTGAATTCTTG+GGG | 0.549232 | 8.1:-4419936 | MS.gene88664:CDS |
| TAGGATGCAGTGCACTCCGT+TGG | 0.555958 | 8.1:+4419281 | None:intergenic |
| ACATTGGTGTGAAGAGTGTA+TGG | 0.556406 | 8.1:+4419898 | None:intergenic |
| TTTGATATAACATGGGGAGA+TGG | 0.571234 | 8.1:-4420266 | MS.gene88664:CDS |
| AAACTACTTCATCTAATGCA+TGG | 0.572457 | 8.1:-4419412 | MS.gene88664:CDS |
| ATGCAGTGCACTCCGTTGGA+AGG | 0.585964 | 8.1:+4419285 | None:intergenic |
| AGGATTCCAAAGAATGGAGT+AGG | 0.588075 | 8.1:+4419804 | None:intergenic |
| TCAGAACTTTGATATAACAT+GGG | 0.603368 | 8.1:-4420273 | MS.gene88664:CDS |
| TGCATATGTTGTAATGAAAG+TGG | 0.604062 | 8.1:+4420310 | None:intergenic |
| TAGGTGTGAAAATCAGCAGT+TGG | 0.604383 | 8.1:+4419823 | None:intergenic |
| CACTCCGTTGGAAGGCCTTG+GGG | 0.610315 | 8.1:+4419293 | None:intergenic |
| AATAAGAGAATTCAAGAACA+TGG | 0.612760 | 8.1:-4419585 | MS.gene88664:CDS |
| GATGATTGGGCAACAAGAGG+CGG | 0.625561 | 8.1:-4419497 | MS.gene88664:CDS |
| GCTGATGATTGGGCAACAAG+AGG | 0.626727 | 8.1:-4419500 | MS.gene88664:CDS |
| CAGAACCAGAGGCTTTGTCG+AGG | 0.627145 | 8.1:+4420201 | None:intergenic |
| AGAACCAGAGGCTTTGTCGA+GGG | 0.631194 | 8.1:+4420202 | None:intergenic |
| ATTTCCCCAAGGCCTTCCAA+CGG | 0.633773 | 8.1:-4419297 | MS.gene88664:CDS |
| GGATTGGAATCCAGAACCAG+AGG | 0.639024 | 8.1:+4420190 | None:intergenic |
| GGTGCTTACACAATGCGTTG+AGG | 0.644219 | 8.1:+4419784 | None:intergenic |
| TTCCAAAGAATCAACCAATG+AGG | 0.645428 | 8.1:-4419544 | MS.gene88664:CDS |
| AAGTCAACAAAGATTGAGTG+AGG | 0.654429 | 8.1:-4419366 | MS.gene88664:CDS |
| CAGAACTTTGATATAACATG+GGG | 0.655512 | 8.1:-4420272 | MS.gene88664:CDS |
| GACATCAAAAGATTTCCCCA+AGG | 0.699970 | 8.1:-4419308 | MS.gene88664:CDS |
| GCAATTATGAGTGTATGCCA+TGG | 0.758165 | 8.1:+4420370 | None:intergenic |
| CGTGCCAAGATACTCGACAA+CGG | 0.804801 | 8.1:-4420242 | MS.gene88664:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !!! | TTTCTTTAAAAATAATCAAT+TGG | - | chr8.1:4419973-4419992 | MS.gene88664:intron | 10.0% |
| !!! | TGTGTTAATTATTTTTACTA+TGG | - | chr8.1:4419654-4419673 | MS.gene88664:intron | 15.0% |
| !! | ACTAATATATAAACATGTGT+CGG | - | chr8.1:4419931-4419950 | MS.gene88664:CDS | 20.0% |
| !! | TATGAGTATGATATTTGAAA+AGG | + | chr8.1:4419888-4419907 | None:intergenic | 20.0% |
| ! | AATAAGAGAATTCAAGAACA+TGG | - | chr8.1:4420063-4420082 | MS.gene88664:intron | 25.0% |
| ! | ATCAGAACTTTGATATAACA+TGG | - | chr8.1:4419374-4419393 | MS.gene88664:CDS | 25.0% |
| ! | ATGTTCTTGAATTCTCTTAT+TGG | + | chr8.1:4420064-4420083 | None:intergenic | 25.0% |
| ! | GAAAGTTCAAATTATACTGT+AGG | + | chr8.1:4419609-4419628 | None:intergenic | 25.0% |
| ! | TATCATACTCATAAAAACAC+TGG | - | chr8.1:4419895-4419914 | MS.gene88664:CDS | 25.0% |
| ! | TCAGAACTTTGATATAACAT+GGG | - | chr8.1:4419375-4419394 | MS.gene88664:CDS | 25.0% |
| !! | AAATTGACTTTGAATTCTTG+GGG | - | chr8.1:4419712-4419731 | MS.gene88664:intron | 25.0% |
| !! | GAAATTGACTTTGAATTCTT+GGG | - | chr8.1:4419711-4419730 | MS.gene88664:intron | 25.0% |
| !! | TGAAATTGACTTTGAATTCT+TGG | - | chr8.1:4419710-4419729 | MS.gene88664:intron | 25.0% |
| AAACTACTTCATCTAATGCA+TGG | - | chr8.1:4420236-4420255 | MS.gene88664:CDS | 30.0% | |
| AATGTGTTTAGTCAAGGAAA+AGG | - | chr8.1:4419768-4419787 | MS.gene88664:intron | 30.0% | |
| AGAGATATTCATTCTTGGAT+TGG | + | chr8.1:4419477-4419496 | None:intergenic | 30.0% | |
| CAAAAACCAATAGAGATAAG+AGG | + | chr8.1:4419318-4419337 | None:intergenic | 30.0% | |
| CAGAACTTTGATATAACATG+GGG | - | chr8.1:4419376-4419395 | MS.gene88664:CDS | 30.0% | |
| GTCATCAAAAGGATCAAATT+GGG | - | chr8.1:4419686-4419705 | MS.gene88664:intron | 30.0% | |
| TCCAAGAATGAATATCTCTT+TGG | - | chr8.1:4419478-4419497 | MS.gene88664:CDS | 30.0% | |
| TGCATATGTTGTAATGAAAG+TGG | + | chr8.1:4419341-4419360 | None:intergenic | 30.0% | |
| TGTCATCAAAAGGATCAAAT+TGG | - | chr8.1:4419685-4419704 | MS.gene88664:intron | 30.0% | |
| TTCAAGAACATGGAATCAAA+AGG | - | chr8.1:4420073-4420092 | MS.gene88664:intron | 30.0% | |
| ! | AATTGAATGCAGTTTTTCTG+TGG | - | chr8.1:4420030-4420049 | MS.gene88664:intron | 30.0% |
| ! | ATACTGTAGGATTAGCATTA+AGG | + | chr8.1:4419596-4419615 | None:intergenic | 30.0% |
| ! | TTTTCCTTGACTAAACACAT+TGG | + | chr8.1:4419769-4419788 | None:intergenic | 30.0% |
| AAGTCAACAAAGATTGAGTG+AGG | - | chr8.1:4420282-4420301 | MS.gene88664:CDS | 35.0% | |
| AGACTTGAGTATATCCTCAT+TGG | + | chr8.1:4420121-4420140 | None:intergenic | 35.0% | |
| GCCAAAGAGATATTCATTCT+TGG | + | chr8.1:4419482-4419501 | None:intergenic | 35.0% | |
| TCTATGGAATGCTGATGATT+GGG | - | chr8.1:4420138-4420157 | MS.gene88664:CDS | 35.0% | |
| TGAGGATATACTCAAGTCTA+TGG | - | chr8.1:4420122-4420141 | MS.gene88664:CDS | 35.0% | |
| TTCATTACAACATATGCAGC+AGG | - | chr8.1:4419343-4419362 | MS.gene88664:CDS | 35.0% | |
| TTCCAAAGAATCAACCAATG+AGG | - | chr8.1:4420104-4420123 | MS.gene88664:intron | 35.0% | |
| TTCCTATAAGATGCAGTGAA+TGG | + | chr8.1:4420205-4420224 | None:intergenic | 35.0% | |
| TTTGATATAACATGGGGAGA+TGG | - | chr8.1:4419382-4419401 | MS.gene88664:CDS | 35.0% | |
| ! | ATCCTCATTGGTTGATTCTT+TGG | + | chr8.1:4420109-4420128 | None:intergenic | 35.0% |
| ! | TGCATGGTTTTCTCAACAAT+TGG | - | chr8.1:4420252-4420271 | MS.gene88664:CDS | 35.0% |
| AGGATTCCAAAGAATGGAGT+AGG | + | chr8.1:4419847-4419866 | None:intergenic | 40.0% | |
| CACACCAATGTGTTTAGTCA+AGG | - | chr8.1:4419762-4419781 | MS.gene88664:intron | 40.0% | |
| CACTGTCCTCTTATCTCTAT+TGG | - | chr8.1:4419309-4419328 | MS.gene88664:CDS | 40.0% | |
| CAGTTGGATCAAACCAAAGA+TGG | + | chr8.1:4419812-4419831 | None:intergenic | 40.0% | |
| CTCCATTCACTGCATCTTAT+AGG | - | chr8.1:4420200-4420219 | MS.gene88664:CDS | 40.0% | |
| GAATTCTTGGGGAATGTAAG+TGG | - | chr8.1:4419723-4419742 | MS.gene88664:intron | 40.0% | |
| GACATCAAAAGATTTCCCCA+AGG | - | chr8.1:4420340-4420359 | MS.gene88664:CDS | 40.0% | |
| GAGAGCAACAATTCCATCTT+TGG | - | chr8.1:4419796-4419815 | MS.gene88664:CDS | 40.0% | |
| GTCTATGGAATGCTGATGAT+TGG | - | chr8.1:4420137-4420156 | MS.gene88664:CDS | 40.0% | |
| TAGGTGTGAAAATCAGCAGT+TGG | + | chr8.1:4419828-4419847 | None:intergenic | 40.0% | |
| TTCACACCTACTCCATTCTT+TGG | - | chr8.1:4419838-4419857 | MS.gene88664:CDS | 40.0% | |
| ! | ACATTGGTGTGAAGAGTGTA+TGG | + | chr8.1:4419753-4419772 | None:intergenic | 40.0% |
| ! | GAATGCAGTTTTTCTGTGGA+TGG | - | chr8.1:4420034-4420053 | MS.gene88664:intron | 40.0% |
| CTTGTACCTGGAAATTCTGC+AGG | - | chr8.1:4419523-4419542 | MS.gene88664:CDS | 45.0% | |
| GCGTTGAGGATTCCAAAGAA+TGG | + | chr8.1:4419853-4419872 | None:intergenic | 45.0% | |
| GGCATGCAGTTGTCATCAAA+AGG | - | chr8.1:4419675-4419694 | MS.gene88664:intron | 45.0% | |
| TTGACCGTTGTCGAGTATCT+TGG | + | chr8.1:4419413-4419432 | None:intergenic | 45.0% | |
| ACAGTGCCTGCAGAATTTCC+AGG | + | chr8.1:4419532-4419551 | None:intergenic | 50.0% | |
| ATGCAGCTCAAGCTTGTACC+TGG | - | chr8.1:4419511-4419530 | MS.gene88664:CDS | 50.0% | |
| ATTTCCCCAAGGCCTTCCAA+CGG | - | chr8.1:4420351-4420370 | MS.gene88664:CDS | 50.0% | |
| CGTGCCAAGATACTCGACAA+CGG | - | chr8.1:4419406-4419425 | MS.gene88664:CDS | 50.0% | |
| GATGATTGGGCAACAAGAGG+CGG | - | chr8.1:4420151-4420170 | MS.gene88664:CDS | 50.0% | |
| GCTGATGATTGGGCAACAAG+AGG | - | chr8.1:4420148-4420167 | MS.gene88664:CDS | 50.0% | |
| GGATTGGAATCCAGAACCAG+AGG | + | chr8.1:4419461-4419480 | None:intergenic | 50.0% | |
| GGTGCTTACACAATGCGTTG+AGG | + | chr8.1:4419867-4419886 | None:intergenic | 50.0% | |
| ! | AGAACCAGAGGCTTTGTCGA+GGG | + | chr8.1:4419449-4419468 | None:intergenic | 50.0% |
| ! | GCGGTCTTGTCAAGACAGAT+TGG | - | chr8.1:4420170-4420189 | MS.gene88664:CDS | 50.0% |
| ATGCAGTGCACTCCGTTGGA+AGG | + | chr8.1:4420366-4420385 | None:intergenic | 55.0% | |
| CTTTCCCTCGACAAAGCCTC+TGG | - | chr8.1:4419442-4419461 | MS.gene88664:CDS | 55.0% | |
| TAGGATGCAGTGCACTCCGT+TGG | + | chr8.1:4420370-4420389 | None:intergenic | 55.0% | |
| ! | CAGAACCAGAGGCTTTGTCG+AGG | + | chr8.1:4419450-4419469 | None:intergenic | 55.0% |
| ! | CTCGACAAAGCCTCTGGTTC+TGG | - | chr8.1:4419448-4419467 | MS.gene88664:CDS | 55.0% |
| CACTCCGTTGGAAGGCCTTG+GGG | + | chr8.1:4420358-4420377 | None:intergenic | 60.0% | |
| GCACTCCGTTGGAAGGCCTT+GGG | + | chr8.1:4420359-4420378 | None:intergenic | 60.0% | |
| TGCACTCCGTTGGAAGGCCT+TGG | + | chr8.1:4420360-4420379 | None:intergenic | 60.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr8.1 | gene | 4419280 | 4420390 | 4419280 | ID=MS.gene88664 |
| chr8.1 | mRNA | 4419280 | 4420390 | 4419280 | ID=MS.gene88664.t1;Parent=MS.gene88664 |
| chr8.1 | exon | 4420106 | 4420390 | 4420106 | ID=MS.gene88664.t1.exon1;Parent=MS.gene88664.t1 |
| chr8.1 | CDS | 4420106 | 4420390 | 4420106 | ID=cds.MS.gene88664.t1;Parent=MS.gene88664.t1 |
| chr8.1 | exon | 4419793 | 4419986 | 4419793 | ID=MS.gene88664.t1.exon2;Parent=MS.gene88664.t1 |
| chr8.1 | CDS | 4419793 | 4419986 | 4419793 | ID=cds.MS.gene88664.t1;Parent=MS.gene88664.t1 |
| chr8.1 | exon | 4419280 | 4419628 | 4419280 | ID=MS.gene88664.t1.exon3;Parent=MS.gene88664.t1 |
| chr8.1 | CDS | 4419280 | 4419628 | 4419280 | ID=cds.MS.gene88664.t1;Parent=MS.gene88664.t1 |
| Gene Sequence |
| Protein sequence |
