AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene89127

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene89127.t1 MTR_4g102460 67.763 152 48 1 1 151 14 165 4.37e-55 191
MS.gene89127.t1 MTR_7g105270 71.141 149 42 1 4 151 1 149 1.38e-53 186
MS.gene89127.t1 MTR_7g034745 95.000 80 3 1 1 79 15 94 1.64e-45 153
MS.gene89127.t1 MTR_7g056297 65.414 133 45 1 4 135 1 133 1.22e-44 160
MS.gene89127.t1 MTR_8g054490 94.505 91 4 1 1 90 15 105 9.73e-41 144
MS.gene89127.t1 MTR_1g105095 62.136 103 38 1 13 114 76 178 5.15e-32 120
MS.gene89127.t1 MTR_1g052140 34.568 162 72 1 251 412 102 229 4.76e-21 96.3
MS.gene89127.t1 MTR_6g045307 32.386 176 100 1 251 426 143 299 8.79e-21 95.9
MS.gene89127.t1 MTR_1361s0010 72.414 58 16 0 78 135 1 58 1.04e-20 91.3
MS.gene89127.t1 MTR_0045s0120 67.416 89 28 1 4 91 1 89 3.29e-20 85.9
MS.gene89127.t1 MTR_7g060240 58.621 87 36 0 1 87 16 102 1.51e-16 75.5
MS.gene89127.t1 MTR_2g076860 23.810 189 101 1 30 218 75 220 1.20e-13 70.5
MS.gene89127.t1 MTR_0003s0410 40.860 93 54 1 246 338 672 763 1.33e-12 70.1
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score

Find 70 sgRNAs with CRISPR-Local

Find 222 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TTCGAACACCTGGTCAAAAT+TGG 0.152405 6.1:-41337202 None:intergenic
AGTTGTGACGCTTCCAATTC+AGG 0.261063 6.1:+41337228 MS.gene89127:CDS
TACCCAAGAAGCATTCTTAT+AGG 0.291800 6.1:+41335312 MS.gene89127:CDS
TGAAGAAGCATGCTTCAATT+TGG 0.320017 6.1:+41334949 MS.gene89127:CDS
GACGCTTCCAATTCAGGAAT+TGG 0.321227 6.1:+41337234 MS.gene89127:CDS
GTTCCTATAAGAATGCTTCT+TGG 0.324794 6.1:-41335315 None:intergenic
TCTCTGAATGATTGTCTTTG+TGG 0.337173 6.1:-41334783 None:intergenic
CAAAGACTCGTTCAACAGTT+TGG 0.337423 6.1:-41334875 None:intergenic
TGTAGCTTTCCTTCAAAATC+AGG 0.348866 6.1:-41334828 None:intergenic
TTACAGTTTCTTGTAACTGC+TGG 0.378263 6.1:-41334674 None:intergenic
AACCGCGCCAATTCCTGAAT+TGG 0.382822 6.1:-41337241 None:intergenic
TTTGTGCTTGCTCGGTCCAT+TGG 0.383472 6.1:-41337125 None:intergenic
TCCGTTCTTACTTAAACATA+AGG 0.393717 6.1:+41337503 MS.gene89127:CDS
CCGCTTACAGATTGCATAAA+GGG 0.395776 6.1:+41337093 MS.gene89127:intron
CCCGCTTACAGATTGCATAA+AGG 0.395951 6.1:+41337092 MS.gene89127:intron
CTGAGAATTCAAATGCTTGA+GGG 0.402246 6.1:-41337430 None:intergenic
TTCCTATAAGAATGCTTCTT+GGG 0.406315 6.1:-41335314 None:intergenic
AAATGCACAACAAGCACAAT+TGG 0.413256 6.1:+41334695 MS.gene89127:CDS
CAATATTGTTGGAGTTGAAC+TGG 0.420257 6.1:-41335242 None:intergenic
GGCATAAAATTCCTTATCAT+AGG 0.421349 6.1:-41337334 None:intergenic
CGTCTAATGACCAATATTGT+TGG 0.422488 6.1:-41335253 None:intergenic
CAGTTCAACTCCAACAATAT+TGG 0.426742 6.1:+41335243 MS.gene89127:CDS
ACTATGCAAATGAAAGTCAT+TGG 0.441211 6.1:+41337582 MS.gene89127:CDS
ATCCAGATGAGTTCGTTGAT+TGG 0.442139 6.1:+41334850 MS.gene89127:CDS
ATCGTCTGATTGCATGTGTT+GGG 0.445325 6.1:-41334721 None:intergenic
AGCCAATCAACGAACTCATC+TGG 0.446536 6.1:-41334852 None:intergenic
AATGACTTTCATTTGCATAG+TGG 0.452688 6.1:-41337580 None:intergenic
TCGAACACCTGGTCAAAATT+GGG 0.461991 6.1:-41337201 None:intergenic
TCATTTGCATAGTGGCAAGT+AGG 0.470951 6.1:-41337572 None:intergenic
CATCGTCTGATTGCATGTGT+TGG 0.472438 6.1:-41334722 None:intergenic
TAGATACTCAACCTATGATA+AGG 0.482983 6.1:+41337323 MS.gene89127:CDS
ACAATAGCTCGTTACCTTGG+TGG 0.486510 6.1:+41335197 MS.gene89127:CDS
CATTTGCATAGTGGCAAGTA+GGG 0.490498 6.1:-41337571 None:intergenic
TTGTTGCAGATGCTCTTAGC+AGG 0.500657 6.1:+41337541 MS.gene89127:CDS
ATGTTGCATGGCTCGGACAA+TGG 0.514494 6.1:-41337355 None:intergenic
GACTCCAATGTTGCATGGCT+CGG 0.516639 6.1:-41337362 None:intergenic
AGAAGCATGCTTCAATTTGG+TGG 0.516736 6.1:+41334952 MS.gene89127:CDS
GCTGAGAATTCAAATGCTTG+AGG 0.526007 6.1:-41337431 None:intergenic
AAAACGAAGCGAGCCCGTGA+AGG 0.545203 6.1:+41334984 MS.gene89127:CDS
TTGTCCGAGCCATGCAACAT+TGG 0.551311 6.1:+41337358 MS.gene89127:CDS
TCAATCGCAGACATGCCGCT+TGG 0.555509 6.1:+41337460 MS.gene89127:CDS
GAAGCATGCTTCAATTTGGT+GGG 0.555767 6.1:+41334953 MS.gene89127:CDS
CACAACTTACTTCGAACACC+TGG 0.555960 6.1:-41337212 None:intergenic
CAAACAATAGCTCGTTACCT+TGG 0.556712 6.1:+41335194 MS.gene89127:CDS
TTCTTACTTAAACATAAGGC+GGG 0.558390 6.1:+41337507 MS.gene89127:CDS
GCCTTATGTTTAAGTAAGAA+CGG 0.567038 6.1:-41337504 None:intergenic
TAAATGTTTCTCCACTCGTA+TGG 0.571332 6.1:-41335291 None:intergenic
AGATGATGCAAATGCAAGGT+AGG 0.571533 6.1:+41337632 MS.gene89127:CDS
ACACCTGGTCAAAATTGGGA+AGG 0.588259 6.1:-41337197 None:intergenic
ATTCAGAGACAACGACATCA+AGG 0.588760 6.1:+41334797 MS.gene89127:CDS
TTTAGTGAAAAGCTCAACAA+TGG 0.590823 6.1:+41337294 MS.gene89127:CDS
CACTAAAGAAGGCTATAGGA+TGG 0.595225 6.1:-41337278 None:intergenic
GTTCTTACTTAAACATAAGG+CGG 0.602881 6.1:+41337506 MS.gene89127:CDS
CATTGGATTTGAGACAGTCA+AGG 0.605582 6.1:+41337599 MS.gene89127:CDS
GGCGCGGTTCTATGTCAAGA+AGG 0.614541 6.1:+41337255 MS.gene89127:CDS
CACCTGGTCAAAATTGGGAA+GGG 0.614981 6.1:-41337196 None:intergenic
AAATTGGGAAGGGCTAGCAC+TGG 0.617784 6.1:-41337186 None:intergenic
TATTCGTTTAGCCATACGAG+TGG 0.618851 6.1:+41335280 MS.gene89127:CDS
TTATCTTGATAATAATGAGA+AGG 0.620206 6.1:-41335065 None:intergenic
TAAAGGGAGATCAATTCCAA+TGG 0.621858 6.1:+41337109 MS.gene89127:CDS
GAAGTGCGACATTCATGAGA+GGG 0.622096 6.1:+41335166 MS.gene89127:CDS
ACAAAGATGATGCAAATGCA+AGG 0.632382 6.1:+41337628 MS.gene89127:CDS
TTGTAAGAATTCACTCCAAG+CGG 0.639382 6.1:-41337475 None:intergenic
GTAATGACTCCAATGTTGCA+TGG 0.642486 6.1:-41337367 None:intergenic
GTGCGACATTCATGAGAGGG+AGG 0.646046 6.1:+41335169 MS.gene89127:CDS
TGAAGTGCGACATTCATGAG+AGG 0.652722 6.1:+41335165 MS.gene89127:CDS
TTCCAATTCAGGAATTGGCG+CGG 0.668895 6.1:+41337239 MS.gene89127:CDS
ATAAGAAGAATTCTCAGCGG+AGG 0.685634 6.1:-41335342 None:intergenic
CGTCAGTGTTTAATCCACCA+AGG 0.695957 6.1:-41335211 None:intergenic
GGGATAAGAAGAATTCTCAG+CGG 0.781762 6.1:-41335345 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TTATCTTGATAATAATGAGA+AGG - chr6.1:41335068-41335087 None:intergenic 20.0%
! AGCATTTGTTCAATTTCTAT+TGG - chr6.1:41336217-41336236 None:intergenic 25.0%
! ATTAGTGTCTTTAATCACTA+AGG + chr6.1:41336111-41336130 MS.gene89127:intron 25.0%
! TATAAAATGAAGCTAATCCA+TGG - chr6.1:41337038-41337057 None:intergenic 25.0%
! TCTAATAAATCATCAAGTCT+AGG - chr6.1:41336550-41336569 None:intergenic 25.0%
! TGTGATGTTATAATAGATAG+TGG + chr6.1:41335737-41335756 MS.gene89127:intron 25.0%
! TTAGTGATTAAAGACACTAA+TGG - chr6.1:41336112-41336131 None:intergenic 25.0%
! TTTGTTCAATTTCTATTGGA+AGG - chr6.1:41336213-41336232 None:intergenic 25.0%
!! TTTCAGTCAAAAGATGTTTT+AGG - chr6.1:41337164-41337183 None:intergenic 25.0%
!!! GATTAGCTTCATTTTATAGA+AGG + chr6.1:41337040-41337059 MS.gene89127:intron 25.0%
AAAAAGCAAGATCAAGACTT+GGG + chr6.1:41335007-41335026 MS.gene89127:CDS 30.0%
AAATCATCAAGTCTAGGTAT+CGG - chr6.1:41336544-41336563 None:intergenic 30.0%
AATGACTTTCATTTGCATAG+TGG - chr6.1:41337583-41337602 None:intergenic 30.0%
ACTACAAATTTGCCAATGAA+TGG - chr6.1:41336766-41336785 None:intergenic 30.0%
ACTATGCAAATGAAAGTCAT+TGG + chr6.1:41337582-41337601 MS.gene89127:CDS 30.0%
ATGAGCTCTTCATCATAAAT+TGG - chr6.1:41335596-41335615 None:intergenic 30.0%
ATTCATTGGCAAATTTGTAG+TGG + chr6.1:41336765-41336784 MS.gene89127:intron 30.0%
GATTTATTAGATCAGTTGCA+TGG + chr6.1:41336559-41336578 MS.gene89127:intron 30.0%
GCCTTATGTTTAAGTAAGAA+CGG - chr6.1:41337507-41337526 None:intergenic 30.0%
GGCATAAAATTCCTTATCAT+AGG - chr6.1:41337337-41337356 None:intergenic 30.0%
GTTCTTACTTAAACATAAGG+CGG + chr6.1:41337506-41337525 MS.gene89127:CDS 30.0%
TAGATACTCAACCTATGATA+AGG + chr6.1:41337323-41337342 MS.gene89127:CDS 30.0%
TCCGTTCTTACTTAAACATA+AGG + chr6.1:41337503-41337522 MS.gene89127:CDS 30.0%
TTCCTATAAGAATGCTTCTT+GGG - chr6.1:41335317-41335336 None:intergenic 30.0%
TTCTTACTTAAACATAAGGC+GGG + chr6.1:41337507-41337526 MS.gene89127:CDS 30.0%
TTTAGTGAAAAGCTCAACAA+TGG + chr6.1:41337294-41337313 MS.gene89127:CDS 30.0%
! AAGATCGATCTTTGAAAAGA+TGG - chr6.1:41336590-41336609 None:intergenic 30.0%
! ATTCTATAAGCTGGTCTATT+AGG - chr6.1:41336340-41336359 None:intergenic 30.0%
! ATTGTAGTTTGTAAGGATGA+GGG - chr6.1:41335826-41335845 None:intergenic 30.0%
! GTAGATATTCCTGATTTTGA+AGG + chr6.1:41334819-41334838 MS.gene89127:CDS 30.0%
! TGTTTCAAATGTCATGGTTT+TGG + chr6.1:41335452-41335471 MS.gene89127:intron 30.0%
! TTCTATAAGCTGGTCTATTA+GGG - chr6.1:41336339-41336358 None:intergenic 30.0%
! TTTTACTCCATCTTTGACAA+AGG - chr6.1:41336032-41336051 None:intergenic 30.0%
! TTTTCACTAAAGAAGGCTAT+AGG - chr6.1:41337285-41337304 None:intergenic 30.0%
!! AGAAGTATCAAGATCAAGTT+TGG + chr6.1:41335909-41335928 MS.gene89127:intron 30.0%
!! TCATGAATTGACTTTGGAAT+AGG - chr6.1:41337003-41337022 None:intergenic 30.0%
!! TTTGGGTTTATTTTCTGTAG+CGG - chr6.1:41335423-41335442 None:intergenic 30.0%
AAACTACAATGGCTCAACAA+AGG + chr6.1:41335836-41335855 MS.gene89127:intron 35.0%
AAATGCACAACAAGCACAAT+TGG + chr6.1:41334695-41334714 MS.gene89127:CDS 35.0%
ACAAAGATGATGCAAATGCA+AGG + chr6.1:41337628-41337647 MS.gene89127:CDS 35.0%
ACCAAGTGTTTCAAATGTCA+TGG + chr6.1:41335446-41335465 MS.gene89127:intron 35.0%
ATCTTGATACTTCTGACCAA+TGG - chr6.1:41335903-41335922 None:intergenic 35.0%
CAATATTGTTGGAGTTGAAC+TGG - chr6.1:41335245-41335264 None:intergenic 35.0%
CAGTTCAACTCCAACAATAT+TGG + chr6.1:41335243-41335262 MS.gene89127:CDS 35.0%
CCAGAGATGGTTTATATGAA+TGG + chr6.1:41336671-41336690 MS.gene89127:intron 35.0%
CCATTCATATAAACCATCTC+TGG - chr6.1:41336674-41336693 None:intergenic 35.0%
CGTCTAATGACCAATATTGT+TGG - chr6.1:41335256-41335275 None:intergenic 35.0%
CTCATCCTTACAAACTACAA+TGG + chr6.1:41335825-41335844 MS.gene89127:intron 35.0%
CTGAGAATTCAAATGCTTGA+GGG - chr6.1:41337433-41337452 None:intergenic 35.0%
GAAAAAGCAAGATCAAGACT+TGG + chr6.1:41335006-41335025 MS.gene89127:CDS 35.0%
GAACATTAGGATAAAGCTCA+TGG - chr6.1:41336170-41336189 None:intergenic 35.0%
GACCAATGGAAAAATTCACA+AGG - chr6.1:41335889-41335908 None:intergenic 35.0%
GATTAAAGACACTAATGGCT+TGG - chr6.1:41336107-41336126 None:intergenic 35.0%
GTTCCTATAAGAATGCTTCT+TGG - chr6.1:41335318-41335337 None:intergenic 35.0%
GTTGAAGAATTGCTGAAGAA+AGG + chr6.1:41336394-41336413 MS.gene89127:intron 35.0%
GTTGGCATAACAATCATGAT+GGG - chr6.1:41336002-41336021 None:intergenic 35.0%
TAAAGGGAGATCAATTCCAA+TGG + chr6.1:41337109-41337128 MS.gene89127:CDS 35.0%
TAAATGTTTCTCCACTCGTA+TGG - chr6.1:41335294-41335313 None:intergenic 35.0%
TAGTGGTAGTTGTGAAAATG+TGG + chr6.1:41335754-41335773 MS.gene89127:intron 35.0%
TATCGGAAATCGATACTTGA+TGG - chr6.1:41336527-41336546 None:intergenic 35.0%
TCTCTGAATGATTGTCTTTG+TGG - chr6.1:41334786-41334805 None:intergenic 35.0%
TGTAGCTTTCCTTCAAAATC+AGG - chr6.1:41334831-41334850 None:intergenic 35.0%
TGTTGGCATAACAATCATGA+TGG - chr6.1:41336003-41336022 None:intergenic 35.0%
TTACAGTTTCTTGTAACTGC+TGG - chr6.1:41334677-41334696 None:intergenic 35.0%
TTAGGATAAAGCTCATGGTT+TGG - chr6.1:41336165-41336184 None:intergenic 35.0%
TTGTAAGAATTCACTCCAAG+CGG - chr6.1:41337478-41337497 None:intergenic 35.0%
! AAAACTCTTTTGTGCTTGCT+CGG - chr6.1:41337136-41337155 None:intergenic 35.0%
! ACCATGACATTTGAAACACT+TGG - chr6.1:41335450-41335469 None:intergenic 35.0%
! ACTGCATTTAAAACCAGAGA+TGG + chr6.1:41336658-41336677 MS.gene89127:intron 35.0%
! ATCGATCTTTGAAAAGATGG+TGG - chr6.1:41336587-41336606 None:intergenic 35.0%
! CATTGTAGTTTGTAAGGATG+AGG - chr6.1:41335827-41335846 None:intergenic 35.0%
! CTCACATCATGAATTGACTT+TGG - chr6.1:41337009-41337028 None:intergenic 35.0%
! CTGCATTTTCTTCGAACATT+AGG - chr6.1:41336183-41336202 None:intergenic 35.0%
! GTTGAGCTTTTCACTAAAGA+AGG - chr6.1:41337292-41337311 None:intergenic 35.0%
! TGAAGAAGCATGCTTCAATT+TGG + chr6.1:41334949-41334968 MS.gene89127:CDS 35.0%
! TGCCTTGTGAATTTTTCCAT+TGG + chr6.1:41335884-41335903 MS.gene89127:intron 35.0%
! TTGAAACACTTGGTAGCTTT+GGG - chr6.1:41335440-41335459 None:intergenic 35.0%
! TTGAGCCATTGTAGTTTGTA+AGG - chr6.1:41335833-41335852 None:intergenic 35.0%
! TTTGAAACACTTGGTAGCTT+TGG - chr6.1:41335441-41335460 None:intergenic 35.0%
!! AGCTTATGAAGAAGTTGATG+AGG + chr6.1:41335529-41335548 MS.gene89127:intron 35.0%
!! ATTTTAGTCCCCAAAAAGGA+TGG + chr6.1:41336457-41336476 MS.gene89127:intron 35.0%
!! CATGCTATTGACTTTATTCC+TGG + chr6.1:41336304-41336323 MS.gene89127:intron 35.0%
!! TACCCAAGAAGCATTCTTAT+AGG + chr6.1:41335312-41335331 MS.gene89127:CDS 35.0%
!! TGCAATTTTAGTCCCCAAAA+AGG + chr6.1:41336453-41336472 MS.gene89127:intron 35.0%
!! TTGGGTTTATTTTCTGTAGC+GGG - chr6.1:41335422-41335441 None:intergenic 35.0%
!! TTTTTGGGGACTAAAATTGC+AGG - chr6.1:41336454-41336473 None:intergenic 35.0%
!!! CTTCTTTTTCAGGTTCATCT+TGG - chr6.1:41335562-41335581 None:intergenic 35.0%
!!! GAAGGTTTTGTAGAAGTACT+AGG - chr6.1:41335392-41335411 None:intergenic 35.0%
!!! TCTAGCTCTTCTTCTTTTTC+AGG - chr6.1:41335572-41335591 None:intergenic 35.0%
!!! TGATCTTGCTTTTTCCTTCA+CGG - chr6.1:41335001-41335020 None:intergenic 35.0%
!!! TTATTTTCTGTAGCGGGTTT+AGG - chr6.1:41335416-41335435 None:intergenic 35.0%
!!! TTGTTTTTACCAAGTCCACT+CGG - chr6.1:41336076-41336095 None:intergenic 35.0%
AAAATTGCAGGAACAGCACA+AGG - chr6.1:41336442-41336461 None:intergenic 40.0%
AAATTGCAGGAACAGCACAA+GGG - chr6.1:41336441-41336460 None:intergenic 40.0%
ACCTACTCCTTTGTCAAAGA+TGG + chr6.1:41336022-41336041 MS.gene89127:intron 40.0%
AGAACCATCACCATCATCAA+AGG + chr6.1:41335503-41335522 MS.gene89127:intron 40.0%
AGAAGATGACATGCATCCAT+TGG - chr6.1:41335947-41335966 None:intergenic 40.0%
ATAAGAAGAATTCTCAGCGG+AGG - chr6.1:41335345-41335364 None:intergenic 40.0%
ATAAGCTTCGCCTTTGATGA+TGG - chr6.1:41335516-41335535 None:intergenic 40.0%
ATCCAGATGAGTTCGTTGAT+TGG + chr6.1:41334850-41334869 MS.gene89127:CDS 40.0%
ATCGTCTGATTGCATGTGTT+GGG - chr6.1:41334724-41334743 None:intergenic 40.0%
ATGTTCTTTCCTAACCAACG+AGG + chr6.1:41336900-41336919 MS.gene89127:intron 40.0%
ATTCAGAGACAACGACATCA+AGG + chr6.1:41334797-41334816 MS.gene89127:CDS 40.0%
CAAACAATAGCTCGTTACCT+TGG + chr6.1:41335194-41335213 MS.gene89127:CDS 40.0%
CAAAGACTCGTTCAACAGTT+TGG - chr6.1:41334878-41334897 None:intergenic 40.0%
CAACAAAGGTAGTGAAGTGA+AGG + chr6.1:41335850-41335869 MS.gene89127:intron 40.0%
CAAGTACTTCGACCATTCAT+TGG + chr6.1:41336751-41336770 MS.gene89127:intron 40.0%
CACTAAAGAAGGCTATAGGA+TGG - chr6.1:41337281-41337300 None:intergenic 40.0%
CAGCAGTGATTATGTAACCT+AGG - chr6.1:41336933-41336952 None:intergenic 40.0%
CATGATGTGAGAAGTTTCCA+TGG + chr6.1:41337018-41337037 MS.gene89127:intron 40.0%
CATTTGCATAGTGGCAAGTA+GGG - chr6.1:41337574-41337593 None:intergenic 40.0%
CCCTTTATGCAATCTGTAAG+CGG - chr6.1:41337096-41337115 None:intergenic 40.0%
CCGCTTACAGATTGCATAAA+GGG + chr6.1:41337093-41337112 MS.gene89127:intron 40.0%
CCTTTATGCAATCTGTAAGC+GGG - chr6.1:41337095-41337114 None:intergenic 40.0%
CTTGATCTACAGCAAACACA+AGG + chr6.1:41336804-41336823 MS.gene89127:intron 40.0%
CTTTATGCAATCTGTAAGCG+GGG - chr6.1:41337094-41337113 None:intergenic 40.0%
GAAGCATGCTTCAATTTGGT+GGG + chr6.1:41334953-41334972 MS.gene89127:CDS 40.0%
GAAGTGGATACCATCAAATC+AGG + chr6.1:41336611-41336630 MS.gene89127:intron 40.0%
GATACCATCAAATCAGGCTT+AGG + chr6.1:41336617-41336636 MS.gene89127:intron 40.0%
GCTGAAGAAAGGACTAATAC+AGG + chr6.1:41336405-41336424 MS.gene89127:intron 40.0%
GCTGAGAATTCAAATGCTTG+AGG - chr6.1:41337434-41337453 None:intergenic 40.0%
GGGATAAGAAGAATTCTCAG+CGG - chr6.1:41335348-41335367 None:intergenic 40.0%
GGTTACATAATCACTGCTGA+AGG + chr6.1:41336934-41336953 MS.gene89127:intron 40.0%
GTAATGACTCCAATGTTGCA+TGG - chr6.1:41337370-41337389 None:intergenic 40.0%
TATTCGTTTAGCCATACGAG+TGG + chr6.1:41335280-41335299 MS.gene89127:CDS 40.0%
TCAAAGATCGATCTTCGAAG+TGG + chr6.1:41336595-41336614 MS.gene89127:intron 40.0%
TCATTTGCATAGTGGCAAGT+AGG - chr6.1:41337575-41337594 None:intergenic 40.0%
TCCATCTTTGACAAAGGAGT+AGG - chr6.1:41336026-41336045 None:intergenic 40.0%
TCGAACACCTGGTCAAAATT+GGG - chr6.1:41337204-41337223 None:intergenic 40.0%
TGTGGACTCATTCTATAAGC+TGG - chr6.1:41336349-41336368 None:intergenic 40.0%
TTCGAACACCTGGTCAAAAT+TGG - chr6.1:41337205-41337224 None:intergenic 40.0%
TTGATTCATGAGACGCATGA+AGG - chr6.1:41336734-41336753 None:intergenic 40.0%
TTGGTGTGATGTTGTTCCAA+TGG + chr6.1:41335928-41335947 MS.gene89127:intron 40.0%
TTTGACAAAGGAGTAGGTGT+TGG - chr6.1:41336020-41336039 None:intergenic 40.0%
! AAATGTGGTGTCGAACTACA+TGG + chr6.1:41335769-41335788 MS.gene89127:intron 40.0%
! AGAAGCATGCTTCAATTTGG+TGG + chr6.1:41334952-41334971 MS.gene89127:CDS 40.0%
! ATCACAAACTTTTCCAGCAG+TGG - chr6.1:41335723-41335742 None:intergenic 40.0%
! CAGACAAGTTTTTCTGACGA+AGG - chr6.1:41335109-41335128 None:intergenic 40.0%
! CATTGGATTTGAGACAGTCA+AGG + chr6.1:41337599-41337618 MS.gene89127:CDS 40.0%
! CTCCAACTTCCATCCTTTTT+GGG - chr6.1:41336469-41336488 None:intergenic 40.0%
! GGAGCATTAGATAACCCAAA+GGG - chr6.1:41336706-41336725 None:intergenic 40.0%
! TCCAACTTCCATCCTTTTTG+GGG - chr6.1:41336468-41336487 None:intergenic 40.0%
! TCTCCAACTTCCATCCTTTT+TGG - chr6.1:41336470-41336489 None:intergenic 40.0%
! TGGAGCATTAGATAACCCAA+AGG - chr6.1:41336707-41336726 None:intergenic 40.0%
!! CTGATCGTTGTCTGTTTTTC+GGG - chr6.1:41335369-41335388 None:intergenic 40.0%
!! GCTGATCGTTGTCTGTTTTT+CGG - chr6.1:41335370-41335389 None:intergenic 40.0%
!! TGATCGTTGTCTGTTTTTCG+GGG - chr6.1:41335368-41335387 None:intergenic 40.0%
!!! GATCTTGCTTTTTCCTTCAC+GGG - chr6.1:41335000-41335019 None:intergenic 40.0%
AATCCTTGTGGAACGTCTTC+TGG - chr6.1:41336262-41336281 None:intergenic 45.0%
AATGGACAGTGATGCCCTTT+GGG + chr6.1:41336689-41336708 MS.gene89127:intron 45.0%
ACAATAGCTCGTTACCTTGG+TGG + chr6.1:41335197-41335216 MS.gene89127:CDS 45.0%
ACACCTGGTCAAAATTGGGA+AGG - chr6.1:41337200-41337219 None:intergenic 45.0%
ACCTAGGAAAGTAACCTCGT+TGG - chr6.1:41336917-41336936 None:intergenic 45.0%
ACGTCTTCTGGTACAACATC+TGG - chr6.1:41336250-41336269 None:intergenic 45.0%
AGCCAATCAACGAACTCATC+TGG - chr6.1:41334855-41334874 None:intergenic 45.0%
AGTTGTGACGCTTCCAATTC+AGG + chr6.1:41337228-41337247 MS.gene89127:CDS 45.0%
CACAACTTACTTCGAACACC+TGG - chr6.1:41337215-41337234 None:intergenic 45.0%
CACCTGGTCAAAATTGGGAA+GGG - chr6.1:41337199-41337218 None:intergenic 45.0%
CATCGTCTGATTGCATGTGT+TGG - chr6.1:41334725-41334744 None:intergenic 45.0%
CCCGCTTACAGATTGCATAA+AGG + chr6.1:41337092-41337111 MS.gene89127:intron 45.0%
CGTCAGTGTTTAATCCACCA+AGG - chr6.1:41335214-41335233 None:intergenic 45.0%
CTTCAGCTGACATTGCATGT+AGG - chr6.1:41335658-41335677 None:intergenic 45.0%
GAAGTGCGACATTCATGAGA+GGG + chr6.1:41335166-41335185 MS.gene89127:CDS 45.0%
GACGCTTCCAATTCAGGAAT+TGG + chr6.1:41337234-41337253 MS.gene89127:CDS 45.0%
GATCTACAGCAAACACAAGG+AGG + chr6.1:41336807-41336826 MS.gene89127:intron 45.0%
GATGCATGTCATCTTCTCCT+TGG + chr6.1:41335950-41335969 MS.gene89127:intron 45.0%
GCATGTTGAATGTCTCGCAT+CGG - chr6.1:41336289-41336308 None:intergenic 45.0%
GGCTCAGTTCAAAGAAGCTT+TGG + chr6.1:41336132-41336151 MS.gene89127:intron 45.0%
GTGATTTCAGCATGCTCTTG+TGG - chr6.1:41336367-41336386 None:intergenic 45.0%
TCCCCAAAAAGGATGGAAGT+TGG + chr6.1:41336464-41336483 MS.gene89127:intron 45.0%
TGAAGTGCGACATTCATGAG+AGG + chr6.1:41335165-41335184 MS.gene89127:CDS 45.0%
TTAACTCCATTACCACCGAG+TGG + chr6.1:41336058-41336077 MS.gene89127:intron 45.0%
TTCCAATTCAGGAATTGGCG+CGG + chr6.1:41337239-41337258 MS.gene89127:CDS 45.0%
TTGTTGCAGATGCTCTTAGC+AGG + chr6.1:41337541-41337560 MS.gene89127:CDS 45.0%
! ACCAACGAGGTTACTTTCCT+AGG + chr6.1:41336913-41336932 MS.gene89127:intron 45.0%
! GCAGTTTTCCATTCATCTCC+TGG - chr6.1:41336643-41336662 None:intergenic 45.0%
! GGAAAGTAACCTCGTTGGTT+AGG - chr6.1:41336912-41336931 None:intergenic 45.0%
! TGTCAGCTGAAGAAGAGCAT+TGG + chr6.1:41335666-41335685 MS.gene89127:intron 45.0%
! TTTTTACCAAGTCCACTCGG+TGG - chr6.1:41336073-41336092 None:intergenic 45.0%
!! ATGAGACGCATGAAGGTACT+TGG - chr6.1:41336727-41336746 None:intergenic 45.0%
!! ATGGTGATGGTTCTTCGTGT+TGG - chr6.1:41335497-41335516 None:intergenic 45.0%
!! CTGAAGAAGAGCATTGGTTG+AGG + chr6.1:41335672-41335691 MS.gene89127:intron 45.0%
!! TTCGCCTTTGATGATGGTGA+TGG - chr6.1:41335510-41335529 None:intergenic 45.0%
AACCGCGCCAATTCCTGAAT+TGG - chr6.1:41337244-41337263 None:intergenic 50.0%
ATCTTCTCCTTGGAAGACCC+TGG + chr6.1:41335960-41335979 MS.gene89127:intron 50.0%
CATCAAATCAGGCTTAGGCC+AGG + chr6.1:41336622-41336641 MS.gene89127:intron 50.0%
CTATTAGGGATGACAGCTCC+AGG - chr6.1:41336325-41336344 None:intergenic 50.0%
GAATGGACAGTGATGCCCTT+TGG + chr6.1:41336688-41336707 MS.gene89127:intron 50.0%
GACTCCAATGTTGCATGGCT+CGG - chr6.1:41337365-41337384 None:intergenic 50.0%
GAGCTCATTGTTGCTGACCA+TGG + chr6.1:41335608-41335627 MS.gene89127:intron 50.0%
GGATGAGGGTGACTTTGTGT+TGG - chr6.1:41335812-41335831 None:intergenic 50.0%
GTACCAGAAGACGTTCCACA+AGG + chr6.1:41336256-41336275 MS.gene89127:intron 50.0%
TGGAATAGGCCAACTGCTGA+TGG - chr6.1:41336989-41337008 None:intergenic 50.0%
TGTTGAATGTCTCGCATCGG+CGG - chr6.1:41336286-41336305 None:intergenic 50.0%
TTGTCCGAGCCATGCAACAT+TGG + chr6.1:41337358-41337377 MS.gene89127:CDS 50.0%
TTTGTGCTTGCTCGGTCCAT+TGG - chr6.1:41337128-41337147 None:intergenic 50.0%
! AAATTGGGAAGGGCTAGCAC+TGG - chr6.1:41337189-41337208 None:intergenic 50.0%
! ATGTTGCATGGCTCGGACAA+TGG - chr6.1:41337358-41337377 None:intergenic 50.0%
! CTGGCCTAAGCCTGATTTGA+TGG - chr6.1:41336624-41336643 None:intergenic 50.0%
!! AGCCCTTCCCAATTTTGACC+AGG + chr6.1:41337194-41337213 MS.gene89127:CDS 50.0%
!! TATTCGAGTTGACCCCAGCA+AGG + chr6.1:41336957-41336976 MS.gene89127:intron 50.0%
!! TCTGTAGCGGGTTTAGGAGA+AGG - chr6.1:41335410-41335429 None:intergenic 50.0%
AAAACGAAGCGAGCCCGTGA+AGG + chr6.1:41334984-41335003 MS.gene89127:CDS 55.0%
AGGTTGAGGCCATCAGCAGT+TGG + chr6.1:41336977-41336996 MS.gene89127:intron 55.0%
AGTTGACCCCAGCAAGGTTG+AGG + chr6.1:41336963-41336982 MS.gene89127:intron 55.0%
CAGCAGTGGTGCAACGAGTA+TGG - chr6.1:41335709-41335728 None:intergenic 55.0%
CATACTCGTTGCACCACTGC+TGG + chr6.1:41335707-41335726 MS.gene89127:intron 55.0%
CGTATTGCCAGGGTCTTCCA+AGG - chr6.1:41335970-41335989 None:intergenic 55.0%
CTGATGGCCTCAACCTTGCT+GGG - chr6.1:41336973-41336992 None:intergenic 55.0%
GCACGACGATCGTATTGCCA+GGG - chr6.1:41335980-41335999 None:intergenic 55.0%
GGCGCGGTTCTATGTCAAGA+AGG + chr6.1:41337255-41337274 MS.gene89127:CDS 55.0%
GGCTTAGGCCAGGAGATGAA+TGG + chr6.1:41336632-41336651 MS.gene89127:intron 55.0%
GTGCGACATTCATGAGAGGG+AGG + chr6.1:41335169-41335188 MS.gene89127:CDS 55.0%
TCAATCGCAGACATGCCGCT+TGG + chr6.1:41337460-41337479 MS.gene89127:CDS 55.0%
TGATCACCAGAGACTCGCCA+TGG - chr6.1:41335628-41335647 None:intergenic 55.0%
TGATGGCCTCAACCTTGCTG+GGG - chr6.1:41336972-41336991 None:intergenic 55.0%
! CCAAGTCCACTCGGTGGTAA+TGG - chr6.1:41336067-41336086 None:intergenic 55.0%
! CCATTACCACCGAGTGGACT+TGG + chr6.1:41336064-41336083 MS.gene89127:intron 55.0%
GCTGATGGCCTCAACCTTGC+TGG - chr6.1:41336974-41336993 None:intergenic 60.0%
GGCACGACGATCGTATTGCC+AGG - chr6.1:41335981-41336000 None:intergenic 60.0%
TGCTGACCATGGCGAGTCTC+TGG + chr6.1:41335619-41335638 MS.gene89127:intron 60.0%
! CGCATCGGCGGTAATCCTTG+TGG - chr6.1:41336274-41336293 None:intergenic 60.0%


Chromosome Type Strat End Strand Name
chr6.1 gene 41334651 41337653 41334651 ID=MS.gene89127
chr6.1 mRNA 41334651 41337653 41334651 ID=MS.gene89127.t1;Parent=MS.gene89127
chr6.1 exon 41334651 41335392 41334651 ID=MS.gene89127.t1.exon1;Parent=MS.gene89127.t1
chr6.1 CDS 41334651 41335392 41334651 ID=cds.MS.gene89127.t1;Parent=MS.gene89127.t1
chr6.1 exon 41337103 41337653 41337103 ID=MS.gene89127.t1.exon2;Parent=MS.gene89127.t1
chr6.1 CDS 41337103 41337653 41337103 ID=cds.MS.gene89127.t1;Parent=MS.gene89127.t1
Gene Sequence

>MS.gene89127

ATGGAAGAAATGAGAAGACAAATCCAGCAGTTACAAGAAACTGTAAATGCACAACAAGCACAATTGGAAGCCCAACACATGCAATCAGACGATGAATCAAGTAGCGAATCATCATCATTAAGAAGTCGTCGTCCACAAAGACAATCATTCAGAGACAACGACATCAAGGTAGATATTCCTGATTTTGAAGGAAAGCTACATCCAGATGAGTTCGTTGATTGGCTCCAAACTGTTGAACGAGTCTTTGAGTATAAAGAGATACTTGAAGAAAAGAAAGTCAAGATCATTGCTGTCAAATTGAAGAAGCATGCTTCAATTTGGTGGGAAAATCTAAAAACGAAGCGAGCCCGTGAAGGAAAAAGCAAGATCAAGACTTGGGAAAAGATGCGTCGAGAACTAAGCAAAAAGTTCTTACCTTCTCATTATTATCAAGATAATTTTATTCAACTACAGAACCTTCGTCAGAAAAACTTGTCTGTAGAAGAATACACTAGAGAATTTGAGAAGTTAATGATGAAGTGCGACATTCATGAGAGGGAGGAGCAAACAATAGCTCGTTACCTTGGTGGATTAAACACTGACGTTGCTCATCCAGTTCAACTCCAACAATATTGGTCATTAGACGATGTTATTCGTTTAGCCATACGAGTGGAGAAACATTTACCCAAGAAGCATTCTTATAGGAACTTTTCCTCCGCTGAGAATTCTTCTTATCCCCGAAAAACAGACAACGATCAGCCTAGTACTTCTACAAAACCTTCTCCTAAACCCGCTACAGAAAATAAACCCAAAGCTACCAAGTGTTTCAAATGTCATGGTTTTGGACATATAGCTTCAAATTGTCCAACACGAAGAACCATCACCATCATCAAAGGCGAAGCTTATGAAGAAGTTGATGAGGAAACAAACCAAGATGAACCTGAAAAAGAAGAAGAGCTAGAGCCAATTTATGATGAAGAGCTCATTGTTGCTGACCATGGCGAGTCTCTGGTGATCAGACGAAGCCTACATGCAATGTCAGCTGAAGAAGAGCATTGGTTGAGGAAAAACATTTTCCATACTCGTTGCACCACTGCTGGAAAAGTTTGTGATGTTATAATAGATAGTGGTAGTTGTGAAAATGTGGTGTCGAACTACATGGTAGAGAAGTTAGAGATGCCAACACAAAGTCACCCTCATCCTTACAAACTACAATGGCTCAACAAAGGTAGTGAAGTGAAGGTTACTAAGCGTTGCCTTGTGAATTTTTCCATTGGTCAGAAGTATCAAGATCAAGTTTGGTGTGATGTTGTTCCAATGGATGCATGTCATCTTCTCCTTGGAAGACCCTGGCAATACGATCGTCGTGCCCATCATGATTGTTATGCCAACACCTACTCCTTTGTCAAAGATGGAGTAAAAATCAAGTTAACTCCATTACCACCGAGTGGACTTGGTAAAAACAAAAATGAGTCCAAGCCATTAGTGTCTTTAATCACTAAGGCTCAGTTCAAAGAAGCTTTGGATGAAGTCCAAACCATGAGCTTTATCCTAATGTTCGAAGAAAATGCAGAAACTGTCCTTCCAATAGAAATTGAACAAATGCTCAGTGAATTTCCAGATGTTGTACCAGAAGACGTTCCACAAGGATTACCGCCGATGCGAGACATTCAACATGCTATTGACTTTATTCCTGGAGCTGTCATCCCTAATAGACCAGCTTATAGAATGAGTCCACAAGAGCATGCTGAAATCACACGTCAAGTTGAAGAATTGCTGAAGAAAGGACTAATACAGGAAAGTGTTAGCCCTTGTGCTGTTCCTGCAATTTTAGTCCCCAAAAAGGATGGAAGTTGGAGAATGTGTGTTGATAGTCGAGCTGTCAACAAGATTACCATCAAGTATCGATTTCCGATACCTAGACTTGATGATTTATTAGATCAGTTGCATGGTGCCACCATCTTTTCAAAGATCGATCTTCGAAGTGGATACCATCAAATCAGGCTTAGGCCAGGAGATGAATGGAAAACTGCATTTAAAACCAGAGATGGTTTATATGAATGGACAGTGATGCCCTTTGGGTTATCTAATGCTCCAAGTACCTTCATGCGTCTCATGAATCAAGTACTTCGACCATTCATTGGCAAATTTGTAGTGGTCTACTTTGACGATATCTTGATCTACAGCAAACACAAGGAGGAACACTTAGAGCAGCTTCGACAAGTGTTACAAACTCTTCGTGATCAAAAACTCTATGCAAATTTGAAGAAATGTTCTTTCCTAACCAACGAGGTTACTTTCCTAGGTTACATAATCACTGCTGAAGGTATTCGAGTTGACCCCAGCAAGGTTGAGGCCATCAGCAGTTGGCCTATTCCAAAGTCAATTCATGATGTGAGAAGTTTCCATGGATTAGCTTCATTTTATAGAAGGTTCATCAAGAATTTTAGTTCTGTTGCTGCCCCGCTTACAGATTGCATAAAGGGAGATCAATTCCAATGGACCGAGCAAGCACAAAAGAGTTTTGACAACCTAAAACATCTTTTGACTGAAACACCAGTGCTAGCCCTTCCCAATTTTGACCAGGTGTTCGAAGTAAGTTGTGACGCTTCCAATTCAGGAATTGGCGCGGTTCTATGTCAAGAAGGCCATCCTATAGCCTTCTTTAGTGAAAAGCTCAACAATGGTAAACTTAGATACTCAACCTATGATAAGGAATTTTATGCCATTGTCCGAGCCATGCAACATTGGAGTCATTACTTGTTGAATAAAGAATTTATTCTTTATTCTGATCATGAAGCCCTCAAGCATTTGAATTCTCAGCAAAAGATCAATCGCAGACATGCCGCTTGGAGTGAATTCTTACAAGCTTATCCGTTCTTACTTAAACATAAGGCGGGAGTTCAAAATGTTGTTGCAGATGCTCTTAGCAGGCGTCATACCCTACTTGCCACTATGCAAATGAAAGTCATTGGATTTGAGACAGTCAAGGAGTTATACAAAGATGATGCAAATGCAAGGTAG
Protein sequence

>MS.gene89127.t1

MEEMRRQIQQLQETVNAQQAQLEAQHMQSDDESSSESSSLRSRRPQRQSFRDNDIKVDIPDFEGKLHPDEFVDWLQTVERVFEYKEILEEKKVKIIAVKLKKHASIWWENLKTKRAREGKSKIKTWEKMRRELSKKFLPSHYYQDNFIQLQNLRQKNLSVEEYTREFEKLMMKCDIHEREEQTIARYLGGLNTDVAHPVQLQQYWSLDDVIRLAIRVEKHLPKKHSYRNFSSAENSSYPRKTDNDQPNCIKGDQFQWTEQAQKSFDNLKHLLTETPVLALPNFDQVFEVSCDASNSGIGAVLCQEGHPIAFFSEKLNNGKLRYSTYDKEFYAIVRAMQHWSHYLLNKEFILYSDHEALKHLNSQQKINRRHAAWSEFLQAYPFLLKHKAGVQNVVADALSRRHTLLATMQMKVIGFETVKELYKDDANAR