AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene90166

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene90166.t1 MTR_5g029940 83.684 190 14 1 100 289 5 177 1.60e-114 327
MS.gene90166.t1 MTR_8g073730 60.066 303 103 3 2 288 781 1081 1.01e-113 352
MS.gene90166.t1 MTR_8g012190 60.738 298 104 4 2 289 771 1065 1.19e-112 349
MS.gene90166.t1 MTR_8g012190 60.738 298 104 4 2 289 777 1071 1.30e-112 349
MS.gene90166.t1 MTR_5g047530 64.015 264 93 1 2 265 782 1043 6.74e-110 342
MS.gene90166.t1 MTR_3g072140 61.314 274 102 3 1 274 605 874 7.28e-106 327
MS.gene90166.t1 MTR_5g047480 64.822 253 79 2 2 254 782 1024 9.23e-105 327
MS.gene90166.t1 MTR_8g012200 57.329 307 106 5 1 289 790 1089 5.13e-104 327
MS.gene90166.t1 MTR_2g437560 60.274 292 95 6 1 289 753 1026 1.36e-102 322
MS.gene90166.t1 MTR_8g012180 54.921 315 113 6 1 289 565 876 1.68e-102 318
MS.gene90166.t1 MTR_8g012180 54.921 315 113 6 1 289 774 1085 5.57e-101 318
MS.gene90166.t1 MTR_5g036240 51.445 346 107 7 1 289 633 974 7.85e-100 313
MS.gene90166.t1 MTR_8g011710 51.775 338 111 6 1 289 801 1135 9.11e-100 316
MS.gene90166.t1 MTR_7g025250 58.224 304 110 5 1 289 790 1091 2.44e-98 311
MS.gene90166.t1 MTR_3g022930 60.674 267 101 3 3 267 788 1052 1.01e-97 311
MS.gene90166.t1 MTR_8g012080 50.432 347 111 6 1 289 778 1121 1.26e-97 310
MS.gene90166.t1 MTR_8g011950 56.679 277 114 3 13 289 774 1044 5.56e-93 296
MS.gene90166.t1 MTR_8g020430 67.431 218 68 3 1 218 757 971 3.61e-88 282
MS.gene90166.t1 MTR_8g011850 64.530 234 80 3 5 237 814 1045 2.22e-87 285
MS.gene90166.t1 MTR_0468s0010 55.513 263 110 4 7 267 758 1015 6.88e-84 273
MS.gene90166.t1 MTR_5g071610 48.607 323 115 6 6 288 788 1099 4.29e-82 268
MS.gene90166.t1 MTR_8g012005 69.318 176 46 1 100 267 12 187 5.31e-81 246
MS.gene90166.t1 MTR_8g037235 59.556 225 77 4 50 265 748 967 3.14e-78 257
MS.gene90166.t1 MTR_8g037220 57.083 240 81 4 25 254 744 971 2.99e-77 256
MS.gene90166.t1 MTR_8g037220 57.083 240 81 4 25 254 523 750 3.03e-77 256
MS.gene90166.t1 MTR_8g011870 60.819 171 58 2 106 267 117 287 7.97e-67 213
MS.gene90166.t1 MTR_7g091540 44.348 230 124 3 4 231 441 668 3.43e-52 181
MS.gene90166.t1 MTR_6g015455 38.889 234 134 2 4 228 1165 1398 1.55e-47 170
MS.gene90166.t1 MTR_6g015455 37.860 243 135 5 19 251 207 443 9.36e-42 154
MS.gene90166.t1 MTR_6g015665 36.552 290 151 4 4 273 866 1142 3.94e-47 169
MS.gene90166.t1 MTR_4g014990 39.506 243 128 4 4 229 810 1050 2.53e-45 164
MS.gene90166.t1 MTR_4g015030 36.496 274 150 6 19 272 845 1114 3.02e-44 160
MS.gene90166.t1 MTR_4g014570 36.145 249 138 3 4 233 850 1096 2.33e-42 155
MS.gene90166.t1 MTR_6g015745 37.826 230 141 2 4 233 826 1053 2.27e-41 152
MS.gene90166.t1 MTR_6g015430 36.667 240 140 3 4 233 835 1072 4.43e-41 151
MS.gene90166.t1 MTR_6g015430 36.667 240 140 3 4 233 642 879 4.88e-41 151
MS.gene90166.t1 MTR_6g015435 37.849 251 136 5 4 247 810 1047 1.28e-40 150
MS.gene90166.t1 MTR_6g015775 41.000 200 113 2 25 224 924 1118 2.99e-40 149
MS.gene90166.t1 MTR_6g015510 39.912 228 128 3 4 224 817 1042 5.02e-40 149
MS.gene90166.t1 MTR_4g015060 37.295 244 132 3 4 228 822 1063 9.98e-40 147
MS.gene90166.t1 MTR_6g015505 34.538 249 143 2 4 233 589 836 1.38e-39 147
MS.gene90166.t1 MTR_7g055970 36.709 237 126 4 8 224 251 483 3.36e-39 144
MS.gene90166.t1 MTR_6g015675 37.247 247 143 4 4 247 248 485 4.57e-39 143
MS.gene90166.t1 MTR_6g015490 38.333 240 134 4 4 231 868 1105 1.08e-38 145
MS.gene90166.t1 MTR_6g015680 35.581 267 146 6 4 247 818 1081 1.47e-38 144
MS.gene90166.t1 MTR_6g015490 38.333 240 134 4 4 231 642 879 1.48e-38 144
MS.gene90166.t1 MTR_4g020590 38.136 236 111 4 25 233 1292 1519 2.00e-38 144
MS.gene90166.t1 MTR_4g020590 38.136 236 111 4 25 233 1285 1512 2.24e-38 144
MS.gene90166.t1 MTR_4g020535 39.271 247 119 7 12 233 760 1000 2.76e-38 144
MS.gene90166.t1 MTR_6g015695 38.525 244 135 4 1 231 822 1063 2.47e-37 140
MS.gene90166.t1 MTR_0552s0040 36.441 236 127 2 12 224 216 451 4.10e-37 139
MS.gene90166.t1 MTR_4g021095 37.668 223 116 2 25 224 1169 1391 6.61e-37 139
MS.gene90166.t1 MTR_6g016000 32.700 263 151 3 4 247 857 1112 1.42e-36 139
MS.gene90166.t1 MTR_6g016000 32.700 263 151 3 4 247 839 1094 1.56e-36 138
MS.gene90166.t1 MTR_4g020550 33.929 280 149 6 3 261 1246 1510 3.34e-34 132
MS.gene90166.t1 MTR_6g015405 34.940 249 134 3 4 233 822 1061 4.98e-34 131
MS.gene90166.t1 MTR_7g088950 35.857 251 137 5 4 234 1289 1535 5.65e-34 131
MS.gene90166.t1 MTR_7g088950 35.857 251 137 5 4 234 1290 1536 5.82e-34 131
MS.gene90166.t1 MTR_7g088950 35.857 251 137 5 4 234 1255 1501 5.85e-34 131
MS.gene90166.t1 MTR_7g088950 35.857 251 137 5 4 234 1255 1501 6.90e-34 131
MS.gene90166.t1 MTR_4g020700 34.630 257 133 7 4 236 1289 1534 6.95e-34 131
MS.gene90166.t1 MTR_7g088950 34.661 251 140 5 4 234 1255 1501 7.10e-34 130
MS.gene90166.t1 MTR_7g088950 34.661 251 140 5 4 234 1289 1535 7.11e-34 130
MS.gene90166.t1 MTR_7g088950 35.857 251 137 5 4 234 1791 2037 8.52e-34 130
MS.gene90166.t1 MTR_4g020700 34.630 257 133 7 4 236 1289 1534 8.75e-34 130
MS.gene90166.t1 MTR_7g088950 35.857 251 137 5 4 234 1757 2003 8.84e-34 130
MS.gene90166.t1 MTR_7g088950 35.857 251 137 5 4 234 1791 2037 1.02e-33 130
MS.gene90166.t1 MTR_4g021057 37.712 236 112 8 25 233 1324 1551 1.06e-33 130
MS.gene90166.t1 MTR_0286s0020 34.728 239 126 4 20 233 300 533 2.22e-33 129
MS.gene90166.t1 MTR_6g015765 44.800 125 68 1 100 224 1 124 2.27e-33 120
MS.gene90166.t1 MTR_4g020850 36.759 253 129 6 25 255 1511 1754 6.19e-33 128
MS.gene90166.t1 MTR_6g015410 36.818 220 117 4 28 228 805 1021 1.32e-32 127
MS.gene90166.t1 MTR_4g023400 34.601 263 146 9 8 256 1297 1547 1.47e-32 127
MS.gene90166.t1 MTR_6g015730 35.833 240 133 4 4 224 1090 1327 4.08e-32 125
MS.gene90166.t1 MTR_3g104170 41.085 129 75 1 100 228 1 128 5.82e-32 117
MS.gene90166.t1 MTR_3g104240 41.085 129 75 1 100 228 1 128 5.82e-32 117
MS.gene90166.t1 MTR_4g021023 35.885 209 130 2 25 233 1080 1284 6.44e-32 125
MS.gene90166.t1 MTR_4g021023 35.885 209 130 2 25 233 1331 1535 7.85e-32 125
MS.gene90166.t1 MTR_3g104130 41.085 129 75 1 100 228 1 128 1.31e-31 116
MS.gene90166.t1 MTR_3g104210 41.085 129 75 1 100 228 1 128 1.31e-31 116
MS.gene90166.t1 MTR_4g021170 37.387 222 115 3 25 224 1285 1504 1.76e-31 124
MS.gene90166.t1 MTR_4g020640 35.897 234 125 5 20 233 1319 1547 2.51e-31 123
MS.gene90166.t1 MTR_6g015600 36.036 222 132 5 8 224 528 744 2.52e-31 123
MS.gene90166.t1 MTR_4g020640 35.897 234 125 5 20 233 1319 1547 2.62e-31 123
MS.gene90166.t1 MTR_0294s0010 36.797 231 129 7 4 233 639 853 3.74e-30 120
MS.gene90166.t1 MTR_6g015595 38.060 134 83 0 100 233 1 134 1.35e-28 109
MS.gene90166.t1 MTR_6g015580 34.914 232 123 7 12 224 809 1031 4.76e-28 114
MS.gene90166.t1 MTR_6g015580 34.914 232 123 7 12 224 643 865 7.46e-28 113
MS.gene90166.t1 MTR_4g020490 38.587 184 106 3 77 256 1503 1683 1.09e-27 113
MS.gene90166.t1 MTR_7g025250 62.136 103 32 2 1 97 790 891 1.42e-25 106
MS.gene90166.t1 MTR_8g012180 58.586 99 38 2 1 97 774 871 5.53e-25 105
MS.gene90166.t1 MTR_4g021030 54.118 85 39 0 140 224 6 90 1.47e-23 94.0
MS.gene90166.t1 MTR_8g011710 55.556 99 41 2 1 97 801 898 1.72e-23 100
MS.gene90166.t1 MTR_4g017740 48.913 92 47 0 140 231 6 97 2.24e-21 89.7
MS.gene90166.t1 MTR_2g450830 40.650 123 67 2 103 224 746 863 1.07e-20 92.4
MS.gene90166.t1 MTR_4g020760 32.922 243 122 9 12 233 407 629 2.18e-20 91.3
MS.gene90166.t1 MTR_4g068200 36.429 140 85 3 99 235 469 607 2.98e-19 88.2
MS.gene90166.t1 MTR_4g015050 34.286 175 94 3 4 159 705 877 3.43e-19 87.8
MS.gene90166.t1 MTR_5g029950 91.111 45 4 0 1 45 247 291 3.66e-18 82.8
MS.gene90166.t1 MTR_6g465280 47.436 78 41 0 145 222 1 78 5.06e-18 78.2
MS.gene90166.t1 MTR_4g014580 51.471 68 32 1 161 228 629 695 2.14e-14 73.6
MS.gene90166.t1 MTR_6g015720 31.915 141 84 5 25 165 700 828 3.49e-14 73.2
MS.gene90166.t1 MTR_5g071610 47.872 94 36 3 6 97 788 870 6.35e-13 69.3
MS.gene90166.t1 MTR_8g012260 54.717 53 23 1 31 83 44 95 2.12e-12 62.8
MS.gene90166.t1 MTR_6g015450 40.260 77 44 1 157 231 37 113 1.46e-11 61.2
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score

Find 42 sgRNAs with CRISPR-Local

Find 73 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TTCATCAACATCTGAAAATA+TGG 0.140719 5.1:-20537682 MS.gene90166:CDS
AGAAGTTCCTTTACCTTTAT+AGG 0.197582 5.1:+20537770 None:intergenic
TCTAGTAACCTTTGTCTTCC+TGG 0.227988 5.1:-20537816 MS.gene90166:CDS
CTTGTAATGATGTCTTTATT+TGG 0.255804 5.1:+20537321 None:intergenic
GGATGCAAAGAGTAATAATA+TGG 0.293783 5.1:-20538571 MS.gene90166:CDS
CCTTCATGGTTAGCCTATAA+AGG 0.313348 5.1:-20537783 MS.gene90166:CDS
CTGGTGACAATTATCCTTCA+TGG 0.316377 5.1:-20537797 MS.gene90166:CDS
TCTCAGAACTTAGAAGTGTT+TGG 0.322786 5.1:-20538521 MS.gene90166:CDS
GTCTCGCTTGTATATCTTGA+TGG 0.329919 5.1:+20537611 None:intergenic
ACAGCTGCATATCTAATATA+TGG 0.330742 5.1:-20537477 MS.gene90166:CDS
GTCATTGTCTTTAGTGTCCT+TGG 0.333928 5.1:-20538592 MS.gene90166:CDS
CTCAGAACTTAGAAGTGTTT+GGG 0.337264 5.1:-20538520 MS.gene90166:CDS
ATGGGAACTGAATGTCTTAC+TGG 0.349127 5.1:-20537663 MS.gene90166:CDS
ATATGGCCATGCAGTAACTA+TGG 0.370474 5.1:-20537460 MS.gene90166:CDS
TTTGTTGATGATCTGTATAA+TGG 0.372386 5.1:-20538453 MS.gene90166:CDS
GATAGCTGCAAGTTTGCTTC+AGG 0.372703 5.1:+20537375 None:intergenic
AAAGAAAGACTTGACATTTG+TGG 0.395740 5.1:+20538396 None:intergenic
GCCATCATTTAACATGAAAC+TGG 0.400431 5.1:-20537430 MS.gene90166:CDS
TCCAGTTTCATGTTAAATGA+TGG 0.449586 5.1:+20537429 None:intergenic
GATAGTAATTGTTGCATGAA+AGG 0.468935 5.1:-20537726 MS.gene90166:CDS
TGGGCGTGGATTGATTGTTA+AGG 0.471862 5.1:-20537502 MS.gene90166:CDS
CAACTTGTTATCGACACTCT+TGG 0.483630 5.1:-20538342 MS.gene90166:CDS
TCATCAACATCTGAAAATAT+GGG 0.483648 5.1:-20537681 MS.gene90166:CDS
CCTTTATAGGCTAACCATGA+AGG 0.496976 5.1:+20537783 None:intergenic
ATTATTACTCTTTGCATCCA+AGG 0.505530 5.1:+20538575 None:intergenic
TGGTTAGCCTATAAAGGTAA+AGG 0.520031 5.1:-20537777 MS.gene90166:CDS
ATGCAACAATTACTATCCTC+AGG 0.537272 5.1:+20537732 None:intergenic
TGCAAGTTATCTTCCTGTGA+TGG 0.546289 5.1:+20537405 None:intergenic
TTAACATCAAATCTAGAGAC+TGG 0.549985 5.1:-20537552 MS.gene90166:CDS
GATGCAAAGAGTAATAATAT+GGG 0.551729 5.1:-20538570 MS.gene90166:CDS
CATGAAACTGGAGCCATCAC+AGG 0.565173 5.1:-20537418 MS.gene90166:CDS
TCTTTATTTGGCTTCCACGA+TGG 0.572000 5.1:+20537333 None:intergenic
CATGAAGGATAATTGTCACC+AGG 0.572511 5.1:+20537798 None:intergenic
TTTAATGATGAAGATTGGAA+GGG 0.583959 5.1:-20537576 MS.gene90166:CDS
CAGTTTCCATAGTTACTGCA+TGG 0.598874 5.1:+20537454 None:intergenic
CAAGAGTGTCGATAACAAGT+TGG 0.623621 5.1:+20538343 None:intergenic
AATTGTCACCAGGAAGACAA+AGG 0.654735 5.1:+20537808 None:intergenic
TCTTGGCAAGAACATATCAC+AGG 0.656511 5.1:-20538325 MS.gene90166:intron
TCTAGAGACTGGTGACAGCG+TGG 0.665473 5.1:-20537541 MS.gene90166:CDS
GAGTTAGTTGAATCTCTGAG+TGG 0.675918 5.1:+20538490 None:intergenic
CAAGATATACAAGCGAGACA+CGG 0.677627 5.1:-20537607 MS.gene90166:CDS
ATGTGAAAATGAAACCATCG+TGG 0.702853 5.1:-20537347 MS.gene90166:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TCTTAATTAATAATAAAGTA+AGG + chr5.1:20537590-20537609 None:intergenic 10.0%
!!! TTTTAAGTAAAAAATACAAA+TGG + chr5.1:20537630-20537649 None:intergenic 10.0%
!! AATTACATAATTAACTAATC+AGG - chr5.1:20538001-20538020 MS.gene90166:intron 15.0%
!! TAATTATTATGCTATTAATC+TGG + chr5.1:20537978-20537997 None:intergenic 15.0%
!! TATTATTAATTAAGAGAACA+TGG - chr5.1:20537595-20537614 MS.gene90166:CDS 15.0%
! CTTGTAATGATGTCTTTATT+TGG + chr5.1:20538557-20538576 None:intergenic 25.0%
! GAATGTAGAAAAACTGAAAA+AGG + chr5.1:20537773-20537792 None:intergenic 25.0%
! GATGCAAAGAGTAATAATAT+GGG - chr5.1:20537305-20537324 MS.gene90166:CDS 25.0%
! GTATAAGATGAAGATAAACT+AGG + chr5.1:20537893-20537912 None:intergenic 25.0%
! GTGTCAATCAAAATTCTAAA+AGG + chr5.1:20537944-20537963 None:intergenic 25.0%
! TCATCAACATCTGAAAATAT+GGG - chr5.1:20538194-20538213 MS.gene90166:intron 25.0%
! TTCATCAACATCTGAAAATA+TGG - chr5.1:20538193-20538212 MS.gene90166:intron 25.0%
! TTTAATGATGAAGATTGGAA+GGG - chr5.1:20538299-20538318 MS.gene90166:intron 25.0%
!! TGTCTTTTAATGATGAAGAT+TGG - chr5.1:20538294-20538313 MS.gene90166:intron 25.0%
!! TTTTAATGATGAAGATTGGA+AGG - chr5.1:20538298-20538317 MS.gene90166:intron 25.0%
!!! TGTTTTAAGAAATAACAGCT+CGG + chr5.1:20537915-20537934 None:intergenic 25.0%
!!! TTTGTTGATGATCTGTATAA+TGG - chr5.1:20537422-20537441 MS.gene90166:CDS 25.0%
AAAAGAAAACAGTCATAGTC+TGG + chr5.1:20537824-20537843 None:intergenic 30.0%
AAAGAAAGACTTGACATTTG+TGG + chr5.1:20537482-20537501 None:intergenic 30.0%
ACAGCTGCATATCTAATATA+TGG - chr5.1:20538398-20538417 MS.gene90166:CDS 30.0%
AGAAGTTCCTTTACCTTTAT+AGG + chr5.1:20538108-20538127 None:intergenic 30.0%
ATTATTACTCTTTGCATCCA+AGG + chr5.1:20537303-20537322 None:intergenic 30.0%
GATAGTAATTGTTGCATGAA+AGG - chr5.1:20538149-20538168 MS.gene90166:intron 30.0%
GGATGCAAAGAGTAATAATA+TGG - chr5.1:20537304-20537323 MS.gene90166:CDS 30.0%
TCAAATAAATCCACATCCTT+GGG + chr5.1:20537663-20537682 None:intergenic 30.0%
TCCAGTTTCATGTTAAATGA+TGG + chr5.1:20538449-20538468 None:intergenic 30.0%
TTAACATCAAATCTAGAGAC+TGG - chr5.1:20538323-20538342 MS.gene90166:intron 30.0%
! GATGGTTTCATTTTCACATT+TGG + chr5.1:20538527-20538546 None:intergenic 30.0%
! TGGAGATTTTTGTAGCTTTT+GGG - chr5.1:20538354-20538373 MS.gene90166:CDS 30.0%
!! AGATCACTTTTGATTGGAAT+GGG - chr5.1:20537503-20537522 MS.gene90166:CDS 30.0%
!!! TCTTTGAGATCACTTTTGAT+TGG - chr5.1:20537497-20537516 MS.gene90166:CDS 30.0%
AAATAAATCCACATCCTTGG+GGG + chr5.1:20537661-20537680 None:intergenic 35.0%
AACAGTCATAGTCTGGAAAA+AGG + chr5.1:20537817-20537836 None:intergenic 35.0%
ATGCAACAATTACTATCCTC+AGG + chr5.1:20538146-20538165 None:intergenic 35.0%
ATGTGAAAATGAAACCATCG+TGG - chr5.1:20538528-20538547 MS.gene90166:CDS 35.0%
CAAATAAATCCACATCCTTG+GGG + chr5.1:20537662-20537681 None:intergenic 35.0%
CTCAAATAAATCCACATCCT+TGG + chr5.1:20537664-20537683 None:intergenic 35.0%
GCCATCATTTAACATGAAAC+TGG - chr5.1:20538445-20538464 MS.gene90166:CDS 35.0%
! GTGGAGATTTTTGTAGCTTT+TGG - chr5.1:20538353-20538372 MS.gene90166:CDS 35.0%
!! CTCAGAACTTAGAAGTGTTT+GGG - chr5.1:20537355-20537374 MS.gene90166:CDS 35.0%
!! GAGATCACTTTTGATTGGAA+TGG - chr5.1:20537502-20537521 MS.gene90166:CDS 35.0%
!! GATCACTTTTGATTGGAATG+GGG - chr5.1:20537504-20537523 MS.gene90166:CDS 35.0%
!! TCTCAGAACTTAGAAGTGTT+TGG - chr5.1:20537354-20537373 MS.gene90166:CDS 35.0%
!! TGGTTAGCCTATAAAGGTAA+AGG - chr5.1:20538098-20538117 MS.gene90166:intron 35.0%
!!! TGTACTTTTTCAAGTTCCTG+AGG - chr5.1:20538127-20538146 MS.gene90166:intron 35.0%
AATCAGGTGCTCATGATACA+GGG - chr5.1:20538017-20538036 MS.gene90166:intron 40.0%
AATTGTCACCAGGAAGACAA+AGG + chr5.1:20538070-20538089 None:intergenic 40.0%
ATATGGCCATGCAGTAACTA+TGG - chr5.1:20538415-20538434 MS.gene90166:CDS 40.0%
ATGGGAACTGAATGTCTTAC+TGG - chr5.1:20538212-20538231 MS.gene90166:intron 40.0%
CAAGATATACAAGCGAGACA+CGG - chr5.1:20538268-20538287 MS.gene90166:intron 40.0%
CAGTTTCCATAGTTACTGCA+TGG + chr5.1:20538424-20538443 None:intergenic 40.0%
CATGAAGGATAATTGTCACC+AGG + chr5.1:20538080-20538099 None:intergenic 40.0%
CCTTTATAGGCTAACCATGA+AGG + chr5.1:20538095-20538114 None:intergenic 40.0%
CTGGTGACAATTATCCTTCA+TGG - chr5.1:20538078-20538097 MS.gene90166:intron 40.0%
GAGTTAGTTGAATCTCTGAG+TGG + chr5.1:20537388-20537407 None:intergenic 40.0%
GTCATTGTCTTTAGTGTCCT+TGG - chr5.1:20537283-20537302 MS.gene90166:CDS 40.0%
GTCTCGCTTGTATATCTTGA+TGG + chr5.1:20538267-20538286 None:intergenic 40.0%
TAATCAGGTGCTCATGATAC+AGG - chr5.1:20538016-20538035 MS.gene90166:intron 40.0%
TCTAGTAACCTTTGTCTTCC+TGG - chr5.1:20538059-20538078 MS.gene90166:intron 40.0%
TCTTGGCAAGAACATATCAC+AGG - chr5.1:20537550-20537569 MS.gene90166:CDS 40.0%
TGCAAGTTATCTTCCTGTGA+TGG + chr5.1:20538473-20538492 None:intergenic 40.0%
TTAAAAGCCAAAAACCCCCA+AGG - chr5.1:20537644-20537663 MS.gene90166:CDS 40.0%
! ATTTTTGTAGCTTTTGGGCG+TGG - chr5.1:20538359-20538378 MS.gene90166:CDS 40.0%
! TCTTTATTTGGCTTCCACGA+TGG + chr5.1:20538545-20538564 None:intergenic 40.0%
!! CAACTTGTTATCGACACTCT+TGG - chr5.1:20537533-20537552 MS.gene90166:CDS 40.0%
!! CAAGAGTGTCGATAACAAGT+TGG + chr5.1:20537535-20537554 None:intergenic 40.0%
!! CCTTCATGGTTAGCCTATAA+AGG - chr5.1:20538092-20538111 MS.gene90166:intron 40.0%
! GATAGCTGCAAGTTTGCTTC+AGG + chr5.1:20538503-20538522 None:intergenic 45.0%
! TGGGCGTGGATTGATTGTTA+AGG - chr5.1:20538373-20538392 MS.gene90166:CDS 45.0%
CATGAAACTGGAGCCATCAC+AGG - chr5.1:20538457-20538476 MS.gene90166:CDS 50.0%
! TCCACATCCTTGGGGGTTTT+TGG + chr5.1:20537654-20537673 None:intergenic 50.0%
GCCAAAAACCCCCAAGGATG+TGG - chr5.1:20537650-20537669 MS.gene90166:CDS 55.0%
TCTAGAGACTGGTGACAGCG+TGG - chr5.1:20538334-20538353 MS.gene90166:CDS 55.0%


Chromosome Type Strat End Strand Name
chr5.1 gene 20537281 20538616 20537281 ID=MS.gene90166
chr5.1 mRNA 20537281 20538616 20537281 ID=MS.gene90166.t1;Parent=MS.gene90166
chr5.1 exon 20538326 20538616 20538326 ID=MS.gene90166.t1.exon1;Parent=MS.gene90166.t1
chr5.1 CDS 20538326 20538616 20538326 ID=cds.MS.gene90166.t1;Parent=MS.gene90166.t1
chr5.1 exon 20537281 20537859 20537281 ID=MS.gene90166.t1.exon2;Parent=MS.gene90166.t1
chr5.1 CDS 20537281 20537859 20537281 ID=cds.MS.gene90166.t1;Parent=MS.gene90166.t1
Gene Sequence

>MS.gene90166

ATGTCATTGTCTTTAGTGTCCTTGGATGCAAAGAGTAATAATATGGGCTATCGATCATCAGTGCTTACAAGTCTCTCAGAACTTAGAAGTGTTTGGGTTCAGTGCCACTCAGAGATTCAACTAACTCAAGAATTAAGAAAATTTGTTGATGATCTGTATAATGGAAACTTTACTGAGTTGAAAACAACATCGCATAGACCACAAATGTCAAGTCTTTCTTTGAGATCACTTTTGATTGGAATGGGGAGTTTCCAACTTGTTATCGACACTCTTGGCAAGAACATATCACAGGTTCTTTCTCTATCTCCTTACTTTATTATTAATTAAGAGAACATGGAACAATTTTCCATTTGTATTTTTTACTTAAAAGCCAAAAACCCCCAAGGATGTGGATTTATTTGAGTATATATCAAATGAACTATATTAAATGATAAGTTTAGAATTGTGATATAAAAGTGAAATGAAGTATTTTTGATACTAATATCAGTTCCTTTTTCAGTTTTTCTACATTCTATCTTCTTTCTTCATTCATACCTTTTTCCAGACTATGACTGTTTTCTTTTCAATTAGCTTTTTCCAGACTATGACTGTTTTCTTTTCAATTAGCTTCCTAGTTTATCTTCATCTTATACCGAGCTGTTATTTCTTAAAACATATTTACCTTTTAGAATTTTGATTGACACTCACAAAAAGTCCAGATTAATAGCATAATAATTAGTTAATTACATAATTAACTAATCAGGTGCTCATGATACAGGGATTGATGACAACTAATTGTTCTAGTAACCTTTGTCTTCCTGGTGACAATTATCCTTCATGGTTAGCCTATAAAGGTAAAGGAACTTCTGTACTTTTTCAAGTTCCTGAGGATAGTAATTGTTGCATGAAAGGAATAATCTTATGTGTTGTTTATTCATCAACATCTGAAAATATGGGAACTGAATGTCTTACTGGTGTCTTGATTATTAATTACACAAAGTGCACCATCAAGATATACAAGCGAGACACGGTTATGTCTTTTAATGATGAAGATTGGAAGGGTTTAACATCAAATCTAGAGACTGGTGACAGCGTGGAGATTTTTGTAGCTTTTGGGCGTGGATTGATTGTTAAGGTCACAGCTGCATATCTAATATATGGCCATGCAGTAACTATGGAAACTGAGCCATCATTTAACATGAAACTGGAGCCATCACAGGAAGATAACTTGCAGTCATCGCCTGAAGCAAACTTGCAGCTATCGCCAAATGTGAAAATGAAACCATCGTGGAAGCCAAATAAAGACATCATTACAAGACTTACAAAGAGAACAAGACAATGTTTGTGCTTGAACTGA
Protein sequence

>MS.gene90166.t1

MSLSLVSLDAKSNNMGYRSSVLTSLSELRSVWVQCHSEIQLTQELRKFVDDLYNGNFTELKTTSHRPQMSSLSLRSLLIGMGSFQLVIDTLGKNISQGLMTTNCSSNLCLPGDNYPSWLAYKGKGTSVLFQVPEDSNCCMKGIILCVVYSSTSENMGTECLTGVLIINYTKCTIKIYKRDTVMSFNDEDWKGLTSNLETGDSVEIFVAFGRGLIVKVTAAYLIYGHAVTMETEPSFNMKLEPSQEDNLQSSPEANLQLSPNVKMKPSWKPNKDIITRLTKRTRQCLCLN