AlfalfaGEDB | MS.gene90207

M. sativa cultivar XinJiangDaYe / MS.gene90207

KEGG: -
GO: -
PFAM: -

Nr:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene90207.t1	XP_003608424.1	91.9	37	3	0	1	37	1	37	1.40E-06	62.8

Swissprot:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score

Trembl:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene90207.t1	G7JV73	91.9	37	3	0	1	37	1	37	9.9e-07	62.8

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network:

Gene1	Gene2	correlation coefficient	p_value	FDR
MS.gene050082	MS.gene90207	0.82681	2.21E-54	-1.69E-46
MS.gene051422	MS.gene90207	0.830262	3.24E-55	-1.69E-46
MS.gene053568	MS.gene90207	0.815632	8.37E-52	-1.69E-46
MS.gene057549	MS.gene90207	0.805504	1.29E-49	-1.69E-46
MS.gene058067	MS.gene90207	0.843104	1.72E-58	-1.69E-46
MS.gene058068	MS.gene90207	0.885082	1.10E-71	-1.69E-46

PPI:

Gene1	Gene2	Type

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene90207.t1	MTR_4g093940	90.000	110	10	1	1	110	1	109	1.98e-46	146
MS.gene90207.t1	MTR_4g093950	89.381	113	11	1	1	113	1	112	2.14e-46	146
MS.gene90207.t1	MTR_4g093910	88.496	113	12	1	1	113	1	112	1.57e-45	144
MS.gene90207.t1	MTR_4g093920	86.364	110	13	2	1	110	1	108	2.55e-43	138
MS.gene90207.t1	MTR_5g064530	64.052	153	28	5	1	129	1	150	6.68e-38	125
MS.gene90207.t1	MTR_5g064510	64.516	124	23	5	1	106	1	121	7.28e-33	112
MS.gene90207.t1	MTR_4g094678	72.152	79	5	2	17	93	1	64	6.78e-25	90.5

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score

Find 46 sgRNAs with CRISPR-Local

Find 50 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AGATTTCCCTGAGGCATATT+TGG	0.193132	4.2:-19767351	None:intergenic
TTTCATCACAACTTTACTTC+TGG	0.306324	4.2:+19767206	MS.gene90207:CDS
CAATAGATGGCGAGCTTCTA+AGG	0.329667	4.2:-19767250	None:intergenic
GTGGGAACGGTTGGTAAGTT+TGG	0.332620	4.2:-19767288	None:intergenic
GTTGGAATGGAAGGAAAGTT+TGG	0.350101	4.2:-19767501	None:intergenic
GTGTTGTTGTTTGCAATAGA+TGG	0.376065	4.2:-19767263	None:intergenic
GAAGGTGGTGGGGAGAGAAA+TGG	0.390913	4.2:-19767540	None:intergenic
TGGGGAGAGAAATGGGATGG+AGG	0.398214	4.2:-19767532	None:intergenic
GGCTAGATGTTGAAGGTGGT+GGG	0.401160	4.2:-19767551	None:intergenic
AAGGTGGTGGGGAGAGAAAT+GGG	0.410900	4.2:-19767539	None:intergenic
TGAGGCATATTTGGAATTGA+AGG	0.434332	4.2:-19767342	None:intergenic
GGAATTGTTGTAGGAAAGCT+AGG	0.436162	4.2:-19767429	None:intergenic
AAATGGGATGGAGGGGAAAG+TGG	0.438380	4.2:-19767523	None:intergenic
TTCAATTCCAAATATGCCTC+AGG	0.454801	4.2:+19767344	MS.gene90207:CDS
GGGCTAGATGTTGAAGGTGG+TGG	0.456957	4.2:-19767552	None:intergenic
GGGAAAGTGGTTGGAATGGA+AGG	0.458956	4.2:-19767510	None:intergenic
GGAAGTGTTGGAATTGTTGT+AGG	0.460698	4.2:-19767438	None:intergenic
GGAGGGGAAAGTGGTTGGAA+TGG	0.481813	4.2:-19767514	None:intergenic
AGTGGTGGCATGGTGAGCTT+AGG	0.486837	4.2:-19767405	None:intergenic
GGGATGGAGGGGAAAGTGGT+TGG	0.490688	4.2:-19767519	None:intergenic
GGGAGAGAAATGGGATGGAG+GGG	0.491154	4.2:-19767530	None:intergenic
TATTTGGAATTGAAGGCAAA+GGG	0.500394	4.2:-19767335	None:intergenic
GGGGAGAGAAATGGGATGGA+GGG	0.504818	4.2:-19767531	None:intergenic
TTTGGCAAAGTGGGAACGGT+TGG	0.528011	4.2:-19767297	None:intergenic
GCTTAAGGGCTAGATGTTGA+AGG	0.534967	4.2:-19767558	None:intergenic
GATGGAATGGCAGTAGGCAA+TGG	0.552623	4.2:-19767378	None:intergenic
GTTGTAGGAAAGCTAGGAAG+TGG	0.563107	4.2:-19767423	None:intergenic
ATGTTGAGAGATGGAAGTGT+TGG	0.563754	4.2:-19767450	None:intergenic
TGGTGGGGAGAGAAATGGGA+TGG	0.578876	4.2:-19767535	None:intergenic
GAAGTGACTGCTGGCAATGG+TGG	0.580562	4.2:-19767474	None:intergenic
GGAAGAGATGGAATGGCAGT+AGG	0.584818	4.2:-19767384	None:intergenic
GAGCTTAGGAAGAGATGGAA+TGG	0.585876	4.2:-19767391	None:intergenic
ATATTTGGAATTGAAGGCAA+AGG	0.589474	4.2:-19767336	None:intergenic
GCTAGATGTTGAAGGTGGTG+GGG	0.597019	4.2:-19767550	None:intergenic
GGAATGGCAGTAGGCAATGG+AGG	0.597414	4.2:-19767375	None:intergenic
AAAGCTAGGAAGTGGTGGCA+TGG	0.597500	4.2:-19767415	None:intergenic
ATGGTGAGCTTAGGAAGAGA+TGG	0.610060	4.2:-19767396	None:intergenic
TTTGGAAGTGAAGTGACTGC+TGG	0.615568	4.2:-19767483	None:intergenic
TTTGGAATTGAAGGCAAAGG+GGG	0.630673	4.2:-19767333	None:intergenic
TCAATTCCAAATATGCCTCA+GGG	0.633758	4.2:+19767345	MS.gene90207:CDS
AGTGAAGTGACTGCTGGCAA+TGG	0.639065	4.2:-19767477	None:intergenic
GTAGGAAAGCTAGGAAGTGG+TGG	0.642479	4.2:-19767420	None:intergenic
AATGGTGGAATGTTGAGAGA+TGG	0.653205	4.2:-19767459	None:intergenic
ATTTGGAATTGAAGGCAAAG+GGG	0.685046	4.2:-19767334	None:intergenic
TAAGGGCTAGATGTTGAAGG+TGG	0.733984	4.2:-19767555	None:intergenic
AATGGAGGAAGATTTCCCTG+AGG	0.807544	4.2:-19767360	None:intergenic

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
	TTTCATCACAACTTTACTTC+TGG	+	chr4.2:19767206-19767225	MS.gene90207:CDS	30.0%
!	ATATTTGGAATTGAAGGCAA+AGG	-	chr4.2:19767339-19767358	None:intergenic	30.0%
!	TATTTGGAATTGAAGGCAAA+GGG	-	chr4.2:19767338-19767357	None:intergenic	30.0%
	TCAATTCCAAATATGCCTCA+GGG	+	chr4.2:19767345-19767364	MS.gene90207:CDS	35.0%
	TTCAATTCCAAATATGCCTC+AGG	+	chr4.2:19767344-19767363	MS.gene90207:CDS	35.0%
!	ATTTGGAATTGAAGGCAAAG+GGG	-	chr4.2:19767337-19767356	None:intergenic	35.0%
!	TGAGGCATATTTGGAATTGA+AGG	-	chr4.2:19767345-19767364	None:intergenic	35.0%
!!	GTGTTGTTGTTTGCAATAGA+TGG	-	chr4.2:19767266-19767285	None:intergenic	35.0%
	AGATTTCCCTGAGGCATATT+TGG	-	chr4.2:19767354-19767373	None:intergenic	40.0%
	GGAATTGTTGTAGGAAAGCT+AGG	-	chr4.2:19767432-19767451	None:intergenic	40.0%
	GTTGGAATGGAAGGAAAGTT+TGG	-	chr4.2:19767504-19767523	None:intergenic	40.0%
!	AATGGTGGAATGTTGAGAGA+TGG	-	chr4.2:19767462-19767481	None:intergenic	40.0%
!	ATGTTGAGAGATGGAAGTGT+TGG	-	chr4.2:19767453-19767472	None:intergenic	40.0%
!	TTTGGAATTGAAGGCAAAGG+GGG	-	chr4.2:19767336-19767355	None:intergenic	40.0%
!!	GGAAGTGTTGGAATTGTTGT+AGG	-	chr4.2:19767441-19767460	None:intergenic	40.0%
!!!	GTTGTTGGTTTTGGCAAAGT+GGG	-	chr4.2:19767309-19767328	None:intergenic	40.0%
!!!	TGTTGTTGGTTTTGGCAAAG+TGG	-	chr4.2:19767310-19767329	None:intergenic	40.0%
	AATGGAGGAAGATTTCCCTG+AGG	-	chr4.2:19767363-19767382	None:intergenic	45.0%
	ATGGTGAGCTTAGGAAGAGA+TGG	-	chr4.2:19767399-19767418	None:intergenic	45.0%
	CAATAGATGGCGAGCTTCTA+AGG	-	chr4.2:19767253-19767272	None:intergenic	45.0%
	GAGCTTAGGAAGAGATGGAA+TGG	-	chr4.2:19767394-19767413	None:intergenic	45.0%
	GTTGTAGGAAAGCTAGGAAG+TGG	-	chr4.2:19767426-19767445	None:intergenic	45.0%
	TAAGGGCTAGATGTTGAAGG+TGG	-	chr4.2:19767558-19767577	None:intergenic	45.0%
	TTTGGAAGTGAAGTGACTGC+TGG	-	chr4.2:19767486-19767505	None:intergenic	45.0%
!!!	TGGTTTTGGCAAAGTGGGAA+CGG	-	chr4.2:19767304-19767323	None:intergenic	45.0%
	AAAGCTAGGAAGTGGTGGCA+TGG	-	chr4.2:19767418-19767437	None:intergenic	50.0%
	AAATGGGATGGAGGGGAAAG+TGG	-	chr4.2:19767526-19767545	None:intergenic	50.0%
	AGTGAAGTGACTGCTGGCAA+TGG	-	chr4.2:19767480-19767499	None:intergenic	50.0%
	GATGGAATGGCAGTAGGCAA+TGG	-	chr4.2:19767381-19767400	None:intergenic	50.0%
	GCTAGATGTTGAAGGTGGTG+GGG	-	chr4.2:19767553-19767572	None:intergenic	50.0%
	GGAAGAGATGGAATGGCAGT+AGG	-	chr4.2:19767387-19767406	None:intergenic	50.0%
	GGCTAGATGTTGAAGGTGGT+GGG	-	chr4.2:19767554-19767573	None:intergenic	50.0%
	GGGAAAGTGGTTGGAATGGA+AGG	-	chr4.2:19767513-19767532	None:intergenic	50.0%
	GTAGGAAAGCTAGGAAGTGG+TGG	-	chr4.2:19767423-19767442	None:intergenic	50.0%
	TTTGGCAAAGTGGGAACGGT+TGG	-	chr4.2:19767300-19767319	None:intergenic	50.0%
!!	AAGGTGGTGGGGAGAGAAAT+GGG	-	chr4.2:19767542-19767561	None:intergenic	50.0%
!!	GTGGGAACGGTTGGTAAGTT+TGG	-	chr4.2:19767291-19767310	None:intergenic	50.0%
	AGTGGTGGCATGGTGAGCTT+AGG	-	chr4.2:19767408-19767427	None:intergenic	55.0%
	GAAGTGACTGCTGGCAATGG+TGG	-	chr4.2:19767477-19767496	None:intergenic	55.0%
	GGAATGGCAGTAGGCAATGG+AGG	-	chr4.2:19767378-19767397	None:intergenic	55.0%
	GGAGGGGAAAGTGGTTGGAA+TGG	-	chr4.2:19767517-19767536	None:intergenic	55.0%
	GGGAGAGAAATGGGATGGAG+GGG	-	chr4.2:19767533-19767552	None:intergenic	55.0%
	GGGCTAGATGTTGAAGGTGG+TGG	-	chr4.2:19767555-19767574	None:intergenic	55.0%
	GGGGAGAGAAATGGGATGGA+GGG	-	chr4.2:19767534-19767553	None:intergenic	55.0%
	TGGGGAGAGAAATGGGATGG+AGG	-	chr4.2:19767535-19767554	None:intergenic	55.0%
	TGGTGGGGAGAGAAATGGGA+TGG	-	chr4.2:19767538-19767557	None:intergenic	55.0%
!!	GAAGGTGGTGGGGAGAGAAA+TGG	-	chr4.2:19767543-19767562	None:intergenic	55.0%
!!!	GGGGGCAGTGTTGTTGGTTT+TGG	-	chr4.2:19767318-19767337	None:intergenic	55.0%
	GGCAAAGGGGGCAGTGTTGT+TGG	-	chr4.2:19767324-19767343	None:intergenic	60.0%
	GGGATGGAGGGGAAAGTGGT+TGG	-	chr4.2:19767522-19767541	None:intergenic	60.0%

Chromosome	Type	Strat	End	Strand	Name
chr4.2	gene	19767189	19767578	19767189	ID=MS.gene90207
chr4.2	mRNA	19767189	19767578	19767189	ID=MS.gene90207.t1;Parent=MS.gene90207
chr4.2	exon	19767189	19767578	19767189	ID=MS.gene90207.t1.exon1;Parent=MS.gene90207.t1
chr4.2	CDS	19767189	19767578	19767189	ID=cds.MS.gene90207.t1;Parent=MS.gene90207.t1

Gene Sequence

>MS.gene90207

ATGGCATCAAGCAAATCTTTCATCACAACTTTACTTCTGGTTGTGACAATGTCAAGCATGACCTTAGAAGCTCGCCATCTATTGCAAACAACAACACAGCCAAACTTACCAACCGTTCCCACTTTGCCAAAACCAACAACACTGCCCCCTTTGCCTTCAATTCCAAATATGCCTCAGGGAAATCTTCCTCCATTGCCTACTGCCATTCCATCTCTTCCTAAGCTCACCATGCCACCACTTCCTAGCTTTCCTACAACAATTCCAACACTTCCATCTCTCAACATTCCACCATTGCCAGCAGTCACTTCACTTCCAAACTTTCCTTCCATTCCAACCACTTTCCCCTCCATCCCATTTCTCTCCCCACCACCTTCAACATCTAGCCCTTAA

Protein sequence

>MS.gene90207.t1

MASSKSFITTLLLVVTMSSMTLEARHLLQTTTQPNLPTVPTLPKPTTLPPLPSIPNMPQGNLPPLPTAIPSLPKLTMPPLPSFPTTIPTLPSLNIPPLPAVTSLPNFPSIPTTFPSIPFLSPPPSTSSP