Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene90757.t1 | XP_013446074.1 | 100 | 67 | 0 | 0 | 1 | 67 | 1 | 67 | 5.60E-28 | 132.9 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene90757.t1 | Q9LYK6 | 42.0 | 50 | 29 | 0 | 15 | 64 | 5 | 54 | 6.7e-06 | 50.8 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene90757.t1 | B7FLB2 | 100.0 | 67 | 0 | 0 | 1 | 67 | 1 | 67 | 4.0e-28 | 132.9 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene049159 | MS.gene90757 | 0.808172 | 3.53E-50 | -1.69E-46 |
| MS.gene057869 | MS.gene90757 | 0.817097 | 3.94E-52 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene90757.t1 | MTR_8g068320 | 100.000 | 67 | 0 | 0 | 1 | 67 | 1 | 67 | 7.13e-41 | 135 |
| MS.gene90757.t1 | MTR_8g068320 | 100.000 | 65 | 0 | 0 | 3 | 67 | 2 | 66 | 3.21e-39 | 131 |
| MS.gene90757.t1 | MTR_6g007797 | 48.980 | 49 | 25 | 0 | 15 | 63 | 7 | 55 | 4.02e-11 | 56.6 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene90757.t1 | AT5G66240 | 67.213 | 61 | 20 | 0 | 7 | 67 | 3 | 63 | 1.31e-22 | 87.8 |
| MS.gene90757.t1 | AT5G66240 | 67.213 | 61 | 20 | 0 | 7 | 67 | 6 | 66 | 1.34e-22 | 88.2 |
| MS.gene90757.t1 | AT5G66240 | 67.213 | 61 | 20 | 0 | 7 | 67 | 3 | 63 | 1.44e-22 | 87.8 |
Find 8 sgRNAs with CRISPR-Local
Find 20 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| AAAAGAAGAGAAAGTTTCTT+TGG | 0.262615 | 8.3:+34948414 | MS.gene90757:CDS |
| GAATGGTAGAATTAGTTCTA+TGG | 0.311519 | 8.3:+34948603 | MS.gene90757:intron |
| TGAAGAAATTCTTCTCAGTA+TGG | 0.369274 | 8.3:+34948444 | MS.gene90757:CDS |
| CGATTATACGATGTTGCTGC+TGG | 0.402567 | 8.3:+34948679 | MS.gene90757:CDS |
| ATTGGTTACGTAATCTTTGA+AGG | 0.456413 | 8.3:-34948479 | None:intergenic |
| ATTCTTCTCAGTATGGAAGT+TGG | 0.458720 | 8.3:+34948451 | MS.gene90757:CDS |
| AGCAACATCGTATAATCGAA+TGG | 0.626818 | 8.3:-34948674 | None:intergenic |
| AATTCATCTCCAACAATGGG+TGG | 0.661924 | 8.3:+34948287 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | AAAAAGAAAATAGTAGAAAT+TGG | - | chr8.3:34948500-34948519 | None:intergenic | 15.0% |
| !!! | TAATCAAATTCAACATTTTA+GGG | + | chr8.3:34948527-34948546 | MS.gene90757:intron | 15.0% |
| !!! | TTAATCAAATTCAACATTTT+AGG | + | chr8.3:34948526-34948545 | MS.gene90757:intron | 15.0% |
| !! | AAAACCAAGTAAAAAAGAAA+CGG | - | chr8.3:34948351-34948370 | None:intergenic | 20.0% |
| ! | AAAAGAAGAGAAAGTTTCTT+TGG | + | chr8.3:34948414-34948433 | MS.gene90757:CDS | 25.0% |
| !!! | TAGTTCTATGGATTTTCATA+GGG | + | chr8.3:34948615-34948634 | MS.gene90757:CDS | 25.0% |
| !!! | TTAGTTCTATGGATTTTCAT+AGG | + | chr8.3:34948614-34948633 | MS.gene90757:CDS | 25.0% |
| !!! | TTGTGCATTTTTTTACAGAA+TGG | + | chr8.3:34948586-34948605 | MS.gene90757:intron | 25.0% |
| ATTGGTTACGTAATCTTTGA+AGG | - | chr8.3:34948482-34948501 | None:intergenic | 30.0% | |
| TGAAGAAATTCTTCTCAGTA+TGG | + | chr8.3:34948444-34948463 | MS.gene90757:CDS | 30.0% | |
| ! | AGGGTTTTTCATAGCATAAT+CGG | + | chr8.3:34948546-34948565 | MS.gene90757:intron | 30.0% |
| ! | ATTTTCGTGTTTTTCTTCTC+AGG | + | chr8.3:34948379-34948398 | MS.gene90757:intron | 30.0% |
| !! | GAATGGTAGAATTAGTTCTA+TGG | + | chr8.3:34948603-34948622 | MS.gene90757:intron | 30.0% |
| AGCAACATCGTATAATCGAA+TGG | - | chr8.3:34948677-34948696 | None:intergenic | 35.0% | |
| ATTCTTCTCAGTATGGAAGT+TGG | + | chr8.3:34948451-34948470 | MS.gene90757:CDS | 35.0% | |
| ! | GGGTTTTTCATAGCATAATC+GGG | + | chr8.3:34948547-34948566 | MS.gene90757:intron | 35.0% |
| !! | ACTTTCTCTTCTTTTTCACC+TGG | - | chr8.3:34948409-34948428 | None:intergenic | 35.0% |
| !!! | CGTTCCGTTTCTTTTTTACT+TGG | + | chr8.3:34948344-34948363 | MS.gene90757:intron | 35.0% |
| ! | TTTTTCTTCTCAGGAACACC+AGG | + | chr8.3:34948388-34948407 | MS.gene90757:intron | 40.0% |
| CGATTATACGATGTTGCTGC+TGG | + | chr8.3:34948679-34948698 | MS.gene90757:CDS | 45.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr8.3 | gene | 34948302 | 34948708 | 34948302 | ID=MS.gene90757 |
| chr8.3 | mRNA | 34948302 | 34948708 | 34948302 | ID=MS.gene90757.t1;Parent=MS.gene90757 |
| chr8.3 | exon | 34948302 | 34948308 | 34948302 | ID=MS.gene90757.t1.exon1;Parent=MS.gene90757.t1 |
| chr8.3 | CDS | 34948302 | 34948308 | 34948302 | ID=cds.MS.gene90757.t1;Parent=MS.gene90757.t1 |
| chr8.3 | exon | 34948401 | 34948492 | 34948401 | ID=MS.gene90757.t1.exon2;Parent=MS.gene90757.t1 |
| chr8.3 | CDS | 34948401 | 34948492 | 34948401 | ID=cds.MS.gene90757.t1;Parent=MS.gene90757.t1 |
| chr8.3 | exon | 34948604 | 34948708 | 34948604 | ID=MS.gene90757.t1.exon3;Parent=MS.gene90757.t1 |
| chr8.3 | CDS | 34948604 | 34948708 | 34948604 | ID=cds.MS.gene90757.t1;Parent=MS.gene90757.t1 |
| Gene Sequence |
| Protein sequence |
