Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene90987.t1 | PNX78574.1 | 85.7 | 572 | 82 | 0 | 1 | 572 | 1 | 572 | 1.30E-292 | 1015.8 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene90987.t1 | Q9XH44 | 74.3 | 171 | 43 | 1 | 784 | 953 | 4 | 174 | 2.5e-69 | 265.4 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene90987.t1 | A0A2K3LJ49 | 85.7 | 572 | 82 | 0 | 1 | 572 | 1 | 572 | 9.7e-293 | 1015.8 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene058381 | MS.gene90987 | 0.812008 | 5.26E-51 | -1.69E-46 |
| MS.gene059953 | MS.gene90987 | 0.801956 | 7.05E-49 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene90987.t1 | MTR_0020s0120 | 98.824 | 170 | 2 | 0 | 784 | 953 | 3 | 172 | 6.89e-117 | 355 |
| MS.gene90987.t1 | MTR_0020s0120 | 94.706 | 170 | 2 | 1 | 784 | 953 | 3 | 165 | 7.92e-109 | 333 |
| MS.gene90987.t1 | MTR_7g104460 | 70.833 | 168 | 48 | 1 | 787 | 953 | 7 | 174 | 4.74e-80 | 257 |
| MS.gene90987.t1 | MTR_2g086270 | 67.262 | 168 | 54 | 1 | 787 | 953 | 6 | 173 | 1.17e-78 | 253 |
| MS.gene90987.t1 | MTR_1g060190 | 62.264 | 159 | 58 | 1 | 787 | 943 | 7 | 165 | 4.10e-68 | 225 |
| MS.gene90987.t1 | MTR_7g084970 | 59.036 | 166 | 68 | 0 | 788 | 953 | 8 | 173 | 6.32e-67 | 221 |
| MS.gene90987.t1 | MTR_6g033040 | 58.333 | 168 | 68 | 1 | 787 | 952 | 7 | 174 | 2.46e-64 | 214 |
| MS.gene90987.t1 | MTR_7g085020 | 57.647 | 170 | 71 | 1 | 784 | 953 | 6 | 174 | 2.13e-61 | 206 |
| MS.gene90987.t1 | MTR_7g006630 | 56.213 | 169 | 73 | 1 | 786 | 953 | 1 | 169 | 5.28e-61 | 205 |
| MS.gene90987.t1 | MTR_7g006690 | 57.485 | 167 | 70 | 1 | 788 | 953 | 8 | 174 | 2.64e-60 | 203 |
| MS.gene90987.t1 | MTR_8g106840 | 54.438 | 169 | 75 | 2 | 786 | 953 | 6 | 173 | 5.53e-58 | 197 |
| MS.gene90987.t1 | MTR_7g085040 | 52.381 | 168 | 76 | 2 | 786 | 953 | 6 | 169 | 1.08e-52 | 182 |
| MS.gene90987.t1 | MTR_2g064580 | 87.037 | 54 | 7 | 0 | 1 | 54 | 549 | 602 | 2.63e-23 | 106 |
| MS.gene90987.t1 | MTR_5g078320 | 46.575 | 73 | 38 | 1 | 881 | 952 | 16 | 88 | 1.53e-15 | 73.2 |
| MS.gene90987.t1 | MTR_3g082730 | 25.283 | 265 | 154 | 6 | 33 | 284 | 35 | 268 | 1.45e-14 | 77.0 |
| MS.gene90987.t1 | MTR_5g048690 | 33.929 | 112 | 73 | 1 | 313 | 423 | 105 | 216 | 7.37e-12 | 66.2 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene90987.t1 | AT5G62040 | 68.023 | 172 | 53 | 2 | 784 | 953 | 3 | 174 | 2.45e-77 | 250 |
| MS.gene90987.t1 | AT5G03840 | 63.158 | 171 | 62 | 1 | 784 | 953 | 7 | 177 | 7.91e-76 | 246 |
| MS.gene90987.t1 | AT2G27550 | 69.822 | 169 | 49 | 2 | 787 | 953 | 7 | 175 | 3.64e-75 | 244 |
| MS.gene90987.t1 | AT1G65480 | 62.275 | 167 | 63 | 0 | 787 | 953 | 7 | 173 | 4.01e-70 | 230 |
| MS.gene90987.t1 | AT1G65480 | 62.805 | 164 | 61 | 0 | 787 | 950 | 51 | 214 | 5.92e-68 | 226 |
| MS.gene90987.t1 | AT4G20370 | 58.683 | 167 | 69 | 0 | 787 | 953 | 7 | 173 | 1.73e-63 | 212 |
| MS.gene90987.t1 | AT1G18100 | 52.941 | 170 | 77 | 2 | 786 | 953 | 5 | 173 | 1.29e-56 | 193 |
| MS.gene90987.t1 | AT4G23160 | 40.909 | 110 | 64 | 1 | 675 | 783 | 312 | 421 | 1.18e-16 | 85.9 |
| MS.gene90987.t1 | AT4G23160 | 40.909 | 110 | 64 | 1 | 675 | 783 | 312 | 421 | 1.18e-16 | 85.9 |
| MS.gene90987.t1 | AT1G34070 | 25.097 | 259 | 165 | 6 | 7 | 252 | 14 | 256 | 4.95e-11 | 65.5 |
Find 0 sgRNAs with CRISPR-Local
Find 0 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| 8272 | gene | 50827 | 59085 | 50827 | ID=MS.gene90987 |
| 8272 | mRNA | 50827 | 59085 | 50827 | ID=MS.gene90987.t1;Parent=MS.gene90987 |
| 8272 | exon | 57372 | 59085 | 57372 | ID=MS.gene90987.t1.exon1;Parent=MS.gene90987.t1 |
| 8272 | CDS | 57372 | 59085 | 57372 | ID=cds.MS.gene90987.t1;Parent=MS.gene90987.t1 |
| 8272 | exon | 56515 | 56822 | 56515 | ID=MS.gene90987.t1.exon2;Parent=MS.gene90987.t1 |
| 8272 | CDS | 56515 | 56822 | 56515 | ID=cds.MS.gene90987.t1;Parent=MS.gene90987.t1 |
| 8272 | exon | 55493 | 55821 | 55493 | ID=MS.gene90987.t1.exon3;Parent=MS.gene90987.t1 |
| 8272 | CDS | 55493 | 55821 | 55493 | ID=cds.MS.gene90987.t1;Parent=MS.gene90987.t1 |
| 8272 | exon | 52394 | 52583 | 52394 | ID=MS.gene90987.t1.exon4;Parent=MS.gene90987.t1 |
| 8272 | CDS | 52394 | 52583 | 52394 | ID=cds.MS.gene90987.t1;Parent=MS.gene90987.t1 |
| 8272 | exon | 51804 | 51865 | 51804 | ID=MS.gene90987.t1.exon5;Parent=MS.gene90987.t1 |
| 8272 | CDS | 51804 | 51865 | 51804 | ID=cds.MS.gene90987.t1;Parent=MS.gene90987.t1 |
| 8272 | exon | 51666 | 51706 | 51666 | ID=MS.gene90987.t1.exon6;Parent=MS.gene90987.t1 |
| 8272 | CDS | 51666 | 51706 | 51666 | ID=cds.MS.gene90987.t1;Parent=MS.gene90987.t1 |
| 8272 | exon | 50827 | 51044 | 50827 | ID=MS.gene90987.t1.exon7;Parent=MS.gene90987.t1 |
| 8272 | CDS | 50827 | 51044 | 50827 | ID=cds.MS.gene90987.t1;Parent=MS.gene90987.t1 |
| Gene Sequence |
| Protein sequence |
